1,566 results on '"VETERINARY immunology"'
Search Results
2. History of Veterinary Immunology in New Zealand.
- Author
-
Buddle, Bryce M
- Subjects
- *
ANIMAL disease control , *IMMUNOLOGY , *VACCINE development , *PARASITIC diseases , *VACCINE trials - Abstract
Knowledge gained from veterinary immunology has played an important role in the control of microbial and parasitic diseases in New Zealand through the development and use of vaccines and diagnostic tests. In this article celebrating the 100th anniversary of the Journal, I follow the development of important discoveries in veterinary immunology which have led to major advances in the control of animal diseases. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
3. Structural study of flavivirus immunomodulator proteins from AEDES AEGYPTI salivary glands.
- Author
-
Abrescia, Nicola A.G, Bioquímica y biología molecular, Biokimika eta biologia molekularra, Martínez Castillo, Ane, Abrescia, Nicola A.G, Bioquímica y biología molecular, Biokimika eta biologia molekularra, and Martínez Castillo, Ane
- Abstract
188 p., Aedes aegypti bacteria-responsive protein 1 (AgBR1) y neutrophil stimulating factor 1 (NeSt1) son dos proteínas salivales del mosquito Aedes aegypti que actúan como potenciadoras de la infección del virus Zika (ZIKV). En este trabajo se ha realizado el análisis estructural de AgBR1 y NeSt1 con el fin de relacionar su estructura con la actividad inmunomoduladora que presentan in vivo. La estructura cristalina de AgBR1 ha sido resuelta mediante cristalografía de rayos-X a una resolución de 1,25 Å mediante reemplazo molecular (MR). La estructura general de la proteína muestra el clásico barril TIM (barril ¿ß8), que pertenece, entre otros, a la familia 18 de las glicosil hidrolasas. La estructura de NeSt1 también ha sido resuelta mediante MR utilizando como modelo la reciente estructura del homólogo LIPS-2 con un 63% (los datos de la estructura no se muestran aquí). Desde el punto de vista funcional, las dos proteínas salivales de mosquito se internalizan en el citoplasma de los macrófagos derivados de la médula ósea de ratón (BMDM). La internalización sugiere la unión de las proteínas a un receptor. Los ensayos de estimulación apoyan que ambas las proteínas de las glándulas salivales del mosquito activan la respuesta inmune innata y la formación de fagosomas (solo por AgBR1, aunque NeSt1 también participa en este proceso) en células BMDM. Los receptores FCGR2A y TLR4 han sido identificados como reguladores aguas arriba de ambas proteínas salivales. Este podría ser un mecanismo empleado por las proteínas para acceder al citoplasma de los macrófagos murinos mediante la formación de fagosomas, activando así a los macrófagos.
- Published
- 2025
4. The role of scavenger receptor MARCO in the immuno-oncology of intrahepatic cholangiocarcinoma: a novel prognostic and therapeutic target
- Author
-
Bañales Asurmendi, Jesús María, Perugorria Montiel, María Jesús, Fisiología, Fisiologia, Agirre Lizaso, Aloña, Bañales Asurmendi, Jesús María, Perugorria Montiel, María Jesús, Fisiología, Fisiologia, and Agirre Lizaso, Aloña
- Abstract
200 p., El colangiocarcinoma (CCA) es un conjunto de tumores que se originan en la vía biliar y debido a su carácter asintomático, se diagnostican en fases muy avanzadas, limitando sustancialmente las posibles opciones terapéuticas. Por ello, existe la urgente necesidad de descubrir los mecanismos moleculares involucrados en el desarrollo y progresión del CCA con el fin de encontrar nuevas dianas terapéuticas, así como biomarcadores para su diagnóstico precoz y para poder predecir su pronóstico. Durante los últimos años, se ha observado que el receptor scavenger MARCO tiene un papel clave en la polarización de los macrófagos y en consecuencia en la respuesta inmune adaptativa. Evidencias recientes en tumores sólidos como el carcinoma de mama, colon y melanoma indican que el receptor MARCO se expresa en macrófagos asociados a tumor (TAMs) tipo M2 inmunosupresores en el microambiente tumoral, contribuyendo a la inmunosupresión y al desarrollo y progresión de estos cánceres. Sin embargo, el papel de MARCO en la etiopatogenia del CCA permanece prácticamente desconocido. Nuestros datos en muestras de pacientes con CCA intrahepático (iCCA) indican que MARCO se encuentra en TAMs involucrados en procesos asociados a la inmunosupresión y a la remodelación de la matriz extracelular. Además, elevados niveles de expresión de MARCO en muestras de iCCA están asociados con un peor pronóstico y también se relacionan con una disfuncionalidad de los linfocitos T. En este sentido, los ratones deficientes de MARCO (Marco-/-) han demostrado tener una protección frente al desarrollo y progresión del iCCA en diferentes modelos experimentales murinos, y estudios proteómicos llevados a cabo con células de iCCA murinas y macrófagos residentes del hígado demuestran una regulación de procesos clave implicados en la colangiocarcinogénesis.
- Published
- 2025
5. Essentials of Veterinary Immunology and Immunopathology
- Author
-
Ramswaroop Singh Chauhan, Yashpal Singh Malik, M. Saminathan, Bhupendra Nath Tripathi, Ramswaroop Singh Chauhan, Yashpal Singh Malik, M. Saminathan, and Bhupendra Nath Tripathi
- Subjects
- Veterinary immunology, Immunopathology
- Abstract
This textbook for graduate veterinary students provides basic concepts of veterinary immunopathology with particular reference to immunopathogenesis. The initial chapters provide up-to-date knowledge on important aspects of immunology, immunodeficiency disorders, hypersensitivity reactions, and autoimmune disorders of various animal species. The book examines different natural and synthetic immunomodulatory products that can modulate the immune system of animals. It discusses the immunopathogenesis and immunopathology of multiple diseases and immunopathological disorders in diverse animal species, including cattle, buffalo, sheep, goat, swine, equine, and laboratory animals. The book also covers the immunopathology of various body systems, including lungs, reproductive system, enteric system, mammary gland, joints, kidneys, and skin. It provides clinical manifestations that are useful in diagnosing immunopathological conditions. The book presents a highly informative text with highquality gross and microscopic photographs, diagrammatic presentations of immunopathological disorders and techniques, making it useful in both classrooms and professional training courses for students, researchers, and professionals alike.
- Published
- 2024
6. Autoimmune Diseases In Domestic Animals - E-Book : Autoimmune Diseases In Domestic Animals - E-Book
- Author
-
Ian R Tizard and Ian R Tizard
- Subjects
- Autoimmune diseases, Domestic animals--Diseases, Veterinary immunology
- Abstract
••Selected for Doody's Core Titles® 2024 in Veterinary Medicine•• With nearly 100 different autoimmune diseases reported to occur in domestic animal species, this evidence-based clinical reference is a must-have for every veterinary practice! Autoimmune Diseases in Domestic Animals, from noted veterinary author Ian R. Tizard, sheds light on both common and rare conditions, including skin diseases, diabetes mellitus, thyroiditis, and hemolytic anemia. Convenient and portable, this single-source review addresses autoimmune diseases in dogs, cats, horses, and livestock species with comprehensive coverage of treatment protocols, diagnostic procedures, and the latest pathogenesis studies. - All-in-one guide covers the basic science, clinical presentation, and treatment of this set of increasingly prevalent animal diseases. - Comprehensive information for each disease prepares students to communicate effectively with clients. - Reviews of the most recent relevant literature provide a clearer understanding of complex autoimmune diseases. - Detailed coverage facilitates the accurate diagnosis and rational treatment of immunologic diseases. - Information on autoimmune mouse strains and other animal models meets the needs of comparative immunology and research students. - Expert author Ian R. Tizard is a well-established veterinary writer who is world-renowned on the topic of immunology in veterinary medicine.
- Published
- 2023
7. Day's Veterinary Immunology : Principles and Practice
- Author
-
Brian Catchpole, Harm HogenEsch, Brian Catchpole, and Harm HogenEsch
- Subjects
- Veterinary immunology
- Abstract
Michael Day's Veterinary Immunology: Principles and Practice is the adopted text in numerous veterinary schools throughout the world. Updated and revised by Brian Catchpole and Harm HogenEsch with advances in knowledge since 2014, this third edition reflects the rapid developments in the field internationally, while preserving the strengths of Day's original writing. It adds numerous case studies demonstrating the clinical context across companion and farm animals.The textbook presents information on commonly used diagnostic test procedures and includes learning objectives at the start and key points at the end of each chapter, standard symbols in diagrams throughout the text to provide continuity, clinical examples and clinicopathological figures throughout, and a glossary of terms and list of commonly used abbreviations. Short animations are viewable via the Support Materials tab on the Routledge webpage, adding a new element of practical application. Exploring the immunological principles of both large and small animals, the book emphasizes immunological principles while applying them to disease processes and to clinical practice. It provides a practical textbook for veterinary students and a handy reference for practitioners.
- Published
- 2023
8. Major Histocompatibility Complex as Marker Assisted Selection for Breeding Immunocompetent Animal.
- Author
-
Brujeni, Gholamreza Nikbakht, Houshmand, Pouya, Esmailnejad, Atefeh, and Abasabadi, Fatemeh
- Subjects
ANIMAL breeding ,MAJOR histocompatibility complex ,VACCINE effectiveness ,DRUG resistance in bacteria ,IMMUNOCOMPETENCE ,VETERINARY immunology - Abstract
Due to the threat of vaccination problems and antibiotic resistance, more appropriate approaches are desired to breed disease resistance animals that are now practiced in cattle, sheep, chicken, and fish farming. Over the recent century, breeding programs based on the productive characteristics have increased animals' overall growth rate and productivity. Despite the benefits, livestock breeding with this method has caused many physiological disorders as well as a reduction in overall immunocompetence. This issue highlights the importance of genetic considerations during the process of breeding. One of the most crucial gene clusters identified in animals is the major histocompatibility complex (MHC), which plays a crucial role in the immune system to distinguishing self from non-self. Recent studies have demonstrated that some MHC regions are highly polymorphic. Also, a significant association between different MHC alleles and susceptibility/resistance to various diseases has been found. This review summarizes the recent research on MHC polymorphism and its association with immune responses in domestic animals. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
9. Rabies immune status of vaccinated and unvaccinated dogs in Kwara State, Nigeria.
- Author
-
J. O., Aiyedun, I. S., Oluboyede, O. B., Daodu, O. O., Oludairo, I. D., Olorunshola, and A. T., Darajat
- Subjects
RABIES ,VETERINARY immunology ,DOG vaccination ,QUALITY assurance ,BLOOD testing - Abstract
Rabies is one of the deadliest zoonoses that have existed for decades, affecting wide range of hosts globally. In Nigeria, there is paucity of data on routine antibody check of antirabies vaccinated dogs as quality assurance. This study was designed to investigate the seroprevalence of canine rabies virus in both vaccinated and unvaccinated dogs within Kwara State, Nigeria. A total of one hundred and eighty-two (182) blood samples collected from dogs were transported to the Virology and Immunology Laboratory of the University of Ilorin Veterinary Teaching Hospital for processing. Sera were harvested from the blood samples and rabies IgG was qualitatively determined by enzyme linked immunosorbent assay (ELISA) technique. Overall seroprevalence of 11% (20/182) was recorded in this study. One hundred and twenty-one (66.5%) and Sixty-one (33.5%) of the dogs used in this study had vaccination and non-vaccination status respectively. The study also revealed that several dogs (78.7%; 48/61) that were said to be vaccinated did not have rabies antibodies while some unvaccinated dogs (5.8%; 7/121) had rabies antibodies. There was a significant (χ2 = 11.177, df = 4, P = 0.025) association between age group and rabies virus antibodies detection. The same significant (χ2 = 9.995, df = 1, P = .002) association was observed for vaccination status of dogs sampled. On the other hand, sex has no significant (χ2 = 0.71, df = 1, P = .790) association with rabies virus antibodies. Poor attitude to vaccination of dogs used for hunting in rural and semi-urban areas was generally observed compared to dogs kept for other purposes in urban areas. There is need for increased rabies awareness campaign, vaccine subsidies, and wider sero-surveillance activities engaging the relevant stakeholders with government sponsorship. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
10. Laboratory Manual of Veterinary Mycology, Microbial Biotechnology and Veterinary Immunology and Serology
- Author
-
Varsha Sharma, Joycee Jogi, Varsha Sharma, and Joycee Jogi
- Subjects
- Veterinary immunology, Veterinary serology, Veterinary mycology, Microbial biotechnology
- Abstract
The present manual has been designed in accordance with the recommendations of New MSVE (Minimum Standards of Veterinary Education) course outline for B.V. Sc. & A. H. graduates. The manual includes Veterinary Mycology, Veterinary Immunology & Serology and Microbial Biotechnology. The text features detailed experimental protocols with comprehensive sections on materials and preparations for all exercises. This comprehensive manual is a reference guide to students which covers various aspects of Veterinary Microbiology ranging from the simplest Lacto phenol Cotton Blue Staining, various immunological techniques such as agglutinations, precipitations, ELISA etc. to various molecular techniques such as PCR, SDS-PAGE etc. It fulfils the fundamental needs of the students while studying Veterinary Microbiology. While the manual is designed for veterinary graduates, it can be recommended for any laboratory or institution involved in diagnostic or research work.
- Published
- 2019
11. Immunology and Vaccination, An Issue of Veterinary Clinics of North America: Small Animal Practice
- Author
-
Amy Stone, Philip H. Kass, Amy Stone, and Philip H. Kass
- Subjects
- Veterinary immunology, Veterinary vaccines
- Abstract
This issue of Veterinary Clinics of North America: Small Animal Practice focuses on Immunology and Vaccination, with topics including: Recent Advances In Vaccine Technologies; Immune System's Response to Vaccination; Current Vaccine Strategies for Dogs and Cats; Update on Therapeutic Vaccines; Common and Newly Recognized Autoimmune Diseases; Adverse Response to Vaccination; Vaccines in Shelters and Group Settings; Evidence vs Belief in Vaccine Recommendations; Effects of Aging on the Immune Response; and Use of Antibody Titer to Determine the Need for Vaccination.
- Published
- 2018
12. Review Therapeutic and Prophylactic Use of Oral, Low-Dose IFNs in Species of Veterinary Interest: Back to the Future.
- Author
-
Frazzini, Sara, Riva, Federica, and Amadori, Massimo
- Subjects
VETERINARY immunology ,CYTOKINES ,IMMUNE response ,AUTOIMMUNE diseases ,MULTIDRUG resistance - Abstract
Cytokines are important molecules that orchestrate the immune response. Given their role, cytokines have been explored as drugs in immunotherapy in the fight against different pathological conditions such as bacterial and viral infections, autoimmune diseases, transplantation and cancer. One of the problems related to their administration consists in the definition of the correct dose to avoid severe side effects. In the 70s and 80s different studies demonstrated the efficacy of cytokines in veterinary medicine, but soon the investigations were abandoned in favor of more profitable drugs such as antibiotics. Recently, the World Health Organization has deeply discouraged the use of antibiotics in order to reduce the spread of multi-drug resistant microorganisms. In this respect, the use of cytokines to prevent or ameliorate infectious diseases has been highlighted, and several studies show the potential of their use in therapy and prophylaxis also in the veterinary field. In this review we aim to review the principles of cytokine treatments, mainly IFNs, and to update the experiences encountered in animals. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
13. Case Studies in Veterinary Immunology
- Author
-
Laurel Gershwin and Laurel Gershwin
- Subjects
- Veterinary immunology
- Abstract
Case Studies in Veterinary Immunology presents basic immunological concepts in the context of actual cases seen in clinics. It is intended for veterinary medicine students, interns, residents, and veterinarians, and serves as a valuable supplement and companion to a variety of core immunology textbooks and courses.The book includes cases describing primary immune system defects, secondary immune system defects, and hypersensitivity and autoimmune disorders, as well as dysproteinemias and lymphoid neoplasia. Drawing on the successful approach of Geha's Case Studies in Immunology, each representative case is preceded by a discussion of the principles underlying that specific immunological mechanism. The case itself includes the presenting complaint (signalment), physical examination findings, pertinent diagnostic laboratory data, diagnosis, and treatment options. In those instances in which a specific disorder occurs in both animals and humans, the differences and similarities in the immunological mechanisms and manifestations of the disease are explored. End of case questions highlight important concepts and serve as a review aid for students. Details on standard vaccines and vaccination schedules, as well as descriptions of the types of assays used for evaluation of the immune system, are included as appendices.
- Published
- 2017
14. 2003 ARS Immunology Research Workshop proceedings
- Author
-
ARS Immunology Research Workshop Bethesda, Md.) 2003, Allen, Tim (John Timothy), 1953, Callahan, Rosemary, Animal Welfare Information Center (U.S.), United States. Agricultural Research Service, U.S. Department of Agriculture, National Agricultural Library, ARS Immunology Research Workshop Bethesda, Md.) 2003, Allen, Tim (John Timothy), 1953, Callahan, Rosemary, Animal Welfare Information Center (U.S.), and United States. Agricultural Research Service
- Subjects
Animals ,Congresses ,Diseases ,Immunologic diseases in animals ,Natural immunity ,Nutritional aspects ,Research ,Treatment ,Veterinary immunology - Published
- 2005
15. β-Glucan-Induced Trained Immunity in Dogs
- Author
-
Simon Paris, Ludivine Chapat, Marion Pasin, Manon Lambiel, Thomas E. Sharrock, Rishabh Shukla, Cecile Sigoillot-Claude, Jeanne-Marie Bonnet, Hervé Poulet, Ludovic Freyburger, and Karelle De Luca
- Subjects
trained immunity ,monocytes/macrophages ,epigenetic ,immuno-metabolism ,veterinary immunology ,comparative immunology ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Several observations in the world of comparative immunology in plants, insects, fish and eventually mammals lead to the discovery of trained immunity in the early 2010's. The first demonstrations provided evidence that innate immune cells were capable of developing memory after a first encounter with some pathogens. Trained immunity in mammals was initially described in monocytes with the Bacille Calmette-Guerin vaccine (BCG) or prototypical agonists like β-glucans. This phenomenon relies on epigenetic and metabolic modifications leading to an enhanced secretion of inflammatory cytokines when the host encounters homologous or heterologous pathogens. The objective of our research was to investigate the trained immunity, well-described in mouse and human, in other species of veterinary importance. For this purpose, we adapted an in vitro model of trained innate immunity in dogs. Blood enriched monocytes were stimulated with β-glucans and we confirmed that it induced an increased production of pro-inflammatory and anti-microbial compounds in response to bacterial stimuli. These results constitute the first demonstration of trained immunity in dogs and confirm its signatures in other mammalian species, with an implication of cellular mechanisms similar to those described in mice and humans regarding cellular epigenetics and metabolic regulations.
- Published
- 2020
- Full Text
- View/download PDF
16. β-Glucan-Induced Trained Immunity in Dogs.
- Author
-
Paris, Simon, Chapat, Ludivine, Pasin, Marion, Lambiel, Manon, Sharrock, Thomas E., Shukla, Rishabh, Sigoillot-Claude, Cecile, Bonnet, Jeanne-Marie, Poulet, Hervé, Freyburger, Ludovic, and De Luca, Karelle
- Subjects
DOG training ,BCG vaccines ,CD14 antigen ,PLANT immunology ,PSYCHONEUROIMMUNOLOGY ,NATURAL immunity ,METABOLIC regulation - Abstract
Several observations in the world of comparative immunology in plants, insects, fish and eventually mammals lead to the discovery of trained immunity in the early 2010's. The first demonstrations provided evidence that innate immune cells were capable of developing memory after a first encounter with some pathogens. Trained immunity in mammals was initially described in monocytes with the Bacille Calmette-Guerin vaccine (BCG) or prototypical agonists like β-glucans. This phenomenon relies on epigenetic and metabolic modifications leading to an enhanced secretion of inflammatory cytokines when the host encounters homologous or heterologous pathogens. The objective of our research was to investigate the trained immunity, well-described in mouse and human, in other species of veterinary importance. For this purpose, we adapted an in vitro model of trained innate immunity in dogs. Blood enriched monocytes were stimulated with β-glucans and we confirmed that it induced an increased production of pro-inflammatory and anti-microbial compounds in response to bacterial stimuli. These results constitute the first demonstration of trained immunity in dogs and confirm its signatures in other mammalian species, with an implication of cellular mechanisms similar to those described in mice and humans regarding cellular epigenetics and metabolic regulations. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
17. Expression regulation and comparative interactome study of ANKRD55, a multiple sclerosis risk modulator
- Author
-
Vandenbroeck, Koen, Neurociencias, Neurozientziak, Mena Lucía, Jorge, Vandenbroeck, Koen, Neurociencias, Neurozientziak, and Mena Lucía, Jorge
- Abstract
215 p., La esclerosis múltiple (EM) es una enfermedad neurodegenerativa y autoinmune que a día de hoy no tiene cura. Se trata de una patología compleja que surge por una combinación de factores genéticos y ambientales. El objetivo de esta tesis es el estudio del gen ANKRD55, considerado de riesgo para la EM, así como de su correspondiente proteína. En el estudio genético se analizó el efecto de varios polimorfismos (SNPs) de ANKRD55 sobre la expresión génica. Se observaron efectos opuestos en linfocitos T CD4+ y células dendríticas derivadas de monocitos de pacientes y donantes sanos. Por otro lado, se caracterizó el interactoma proteico de ANKRD55 en cuatro líneas celulares, inmunes y neurológicas, de importancia en la patología de la EM. Entre los resultados, se identificaron proteínas de la familia 14-3-3 unidas a ANKRD55 en todos los tipos celulares analizados, y proteínas del transporte intraflagelar en la línea de microglía. Los posteriores análisis bioinformáticos, basados en los resultados experimentales, revelaron la potencial implicación de ANKRD55 en diversos mecanismos biológicos y moleculares como la sinapsis neuroinmune y la regulación del ciclo celular. Los resultados de estas tesis aumentan el conocimiento sobre el locus genético de ANKRD55 asociado a la EM y su posible función.
- Published
- 2023
18. Imunologia Veterinária
- Author
-
Tizard, Ian R. and Tizard, Ian R.
- Subjects
- Veterinary immunology
- Abstract
Conheça Imunologia Veterinária 9ª edição para estudantes de veterinária e médicos veterinários. Esta nova edição traz uma apresentação direta dos princípios imunológicos básicos com informação clara sobre as mais importantes doenças e respostas imunológicas observadas em animais domésticos.Com uma cobertura clara sobre vacinas e sua utilização, alergias e doenças alérgicas, autoimunidade e imunodeficiências, incluindo questões imunológicas encontradas na prática.Uma seção totalmente atualizada em imunidade inata, incluindo novos capítulos sobre células NK (natural killer) e respostas sistêmicas à infecção.Mais de 500 diagramas e imagens coloridas demonstram e esclarecem visualmente questões complexas, novas informações sobre genômica e técnicas de diagnóstico molecular esclarecem como o emergente campo da genômica apresenta um impacto na resistência a doenças e no diagnóstico e tratamento de doenças infecciosas e imunológicas.
- Published
- 2014
19. The molecular basis of bovine dendritic cell functions
- Author
-
Gliddon, Daniel
- Subjects
636 ,Veterinary immunology - Published
- 2002
20. Veterinary Immunology - E-Book : Veterinary Immunology - E-Book
- Author
-
Ian R Tizard and Ian R Tizard
- Subjects
- Veterinary immunology
- Abstract
The only complete resource on immunology for veterinary students and practitioners, Veterinary Immunology: An Introduction features a straightforward presentation of basic immunologic principles with comprehensive information on the most significant immunological diseases and responses seen in domestic animals. This meticulously updated new edition explores the latest advances in the field and provides a wealth of clinical examples that illustrate and clarify important concepts. - Comprehensive coverage of vaccines and vaccine usage, allergies and allergic diseases, and autoimmunity and immunodeficiencies, prepare you for the multiple immunologic issues you will encounter in practice. - A wealth of clinical examples clearly illustrate key concepts and offer practical strategies for diagnosing and treating immunologic disorders in the clinical setting. - More than 500 full-color diagrams and illustrations visually demonstrate and clarify complex issues. - Completely updated section on innate immunity includes new chapters on natural killer (NK) cells and systemic responses to infection to ensure you have the most up-to-date information. - New information on genomics and molecular diagnostic techniques explores how the emerging field of genomics impacts disease resistance and immunology in general, as well as the diagnosis and treatment of immunological and infectious diseases. - Updated content provides new information on well-recognized older diseases such as rheumatoid arthritis, systemic lupus, and inflammatory bowel disease, as well as current information on new diseases such as devil facial tumor disease and bovine neonatal pancytopenia. - Expanded coverage brings you the latest knowledge on resistance to infection, such as vaccine usage, especially with respect to duration of immunity, the effects of key vitamins and lipids on immune responses, the effects of old age on immunity, and both antiviral and parasitic immunity. - Diagnostic tests described throughout the text include a new section on the analysis of ELISA test data, as well as a brief summary of molecular diagnostic techniques. - Coverage reflecting a significant change in the overall view of immunology provides you with the foundational knowledge needed to grasp the broad pattern of immunologic reactions and understand how the immune system functions as an interconnected network, rather than a series of independent pathways. - New discussions of the critical importance of commensal bacteria and intestinal flora explain help you understand the importance of this normal flora with respect to antibacterial immunity, allergies, and autoimmunity, while at the same time providing a broader view of the animal body and its microflora as a'superorganism.'- A discussion of the importance of adipose tissue in immunity and inflammation addresses the epidemic of obesity in domestic pets and the extraordinary growth rates expected of domestic livestock. - The section on inflammatory mechanisms has been divided into separate chapters focusing on the detection of invaders and the mediators of inflammation to incorporate the vast amount of new information on pattern recognition receptors and the ways in which they warn the body of microbial invasion.
- Published
- 2013
21. Development and characterization of polyclonal antibody against human kappa light chain in rabbit.
- Author
-
Esparvarinha, Mojgan, Nickho, Hamid, Aghebati-Maleki, Leili, Abdolalizadeh, Jalal, Nasiri, Hadi, Valedkarimi, Zahra, and Majidi, Jafar
- Subjects
IMMUNOGLOBULIN G ,RABBITS ,VETERINARY immunology ,ION exchange chromatography ,AFFINITY chromatography ,ENZYME-linked immunosorbent assay - Abstract
Polyclonal antibodies against kappa light chain are used to diagnose diseases producing free light chain. The kappa and lambda light chains are products of immunoglobulin synthesis and released into the circulation in minor amounts such as serum, cerebrospinal fluid, urine and synovial fluid in normal condition. The purpose of this study was the production and purification of polyclonal immunoglobulin G (IgG) against human kappa light chains. In this study, early human IgG was purified by ion-exchange chromatography, reduced with Dithiothreitol and heavy and light chains were separated with size-exclusion chromatography. Afterward, affinity chromatography with protein L Sepharose at pH 2.00 was displayed to be a dominant condition for the separation and purification of the kappa light chain of immunoglobulins from human serum. Eventually, the rabbit was immunized by human kappa light chains. The rabbit IgG was purified and labeled with horseradish peroxidase (HRP). Direct enzyme-linked immunosorbent assay was planned to determine the titer of HRP conjugated rabbit IgG against the human kappa light chain. The optimum titer of anti-kappa IgG was 1:16000. At the result, purified polyclonal anti-kappa is useful tool in biomedical and biochemical researches and diagnostic kits. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
22. Longitudinal surveillance of rotavirus A genotypes circulating in a high milk yield dairy cattle herd after the introduction of a rotavirus vaccine.
- Author
-
Fritzen, Juliana T.T., Oliveira, Marcos V., Lorenzetti, Elis, Miyabe, Flávia M., Viziack, Mariana P., Rodrigues, Carlos A., Ayres, Henderson, Alfieri, Alice F., and Alfieri, Amauri A.
- Subjects
- *
ROTAVIRUSES , *MILK yield , *VIRUS diseases in cattle , *GENOTYPES , *VETERINARY virology , *VETERINARY immunology - Abstract
Highlights • RVA vaccination program reduces the frequency and intensity of diarrhea in dairy calves. • The vaccination immune pressure can select specific genotypes in RVA field strains. • RVA genotype G10P[11] in fecal samples of calves from G6P[5] vaccinated dairy cattle herds. Abstract Worldwide, neonatal diarrhea is one of the most important health issues affecting dairy calves, and rotavirus A (RVA) is one of its primary causes. Among the measures to mitigate the risk of diarrhea outbreaks, cow vaccination stands out as one of the most important. However, the immune pressure resulting from routine vaccination may be able to select specific G and P genotypes in RVA field strains. This study aimed to determine the frequency and intensity of neonatal diarrhea and the incidence of RVA and attempted to monitor the G and P genotypes present in the RVA strains circulating in a high milk yield cattle herd vaccinated with RVA G6P[5] strain. Fecal samples (n = 1220) from 122 Holstein heifer calves between 0–30 days old that were born from RVA-vaccinated cows were collected at 10 different time points, regardless of the presence or absence of diarrhea. The presence of RVA in fecal samples was determined by the polyacrylamide gel electrophoresis (PAGE) technique and confirmed by reverse transcription polymerase chain reaction (RT-PCR). G and P amplicons from 10 RVA-positive fecal samples from calves of different ages and collections were subjected to nucleotide sequencing. The proportion of the calves and fecal samples that were positive for RVA were 62.3% (76/122) and 8.1% (99/1220), respectively. Using sequence analysis, all 10 RVA field strains presented genotype G10P[11]. The protection of G6P[5] vaccination is clear, as this genotype was not detected in this study, and it is known that vaccination against RVA reduces the incidence of diarrhea independent of genotype involved. This result demonstrates the importance of epidemiological monitoring of RVA genotypes circulating in vaccinated dairy cattle herds to the early detection of new potential pathogenic RVA strains. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
23. Plasmid-located dfrA14 gene in Pasteurella multocida isolates from three different pig-producing farms in Germany.
- Author
-
Niemann, Lisa, Feudi, Claudia, Eichhorn, Inga, Hanke, Dennis, Müller, Petra, Brauns, Jasmin, Nathaus, Rolf, Schäkel, Franziska, Höltig, Doris, Wendt, Michael, Kadlec, Kristina, and Schwarz, Stefan
- Subjects
- *
PASTEURELLA multocida , *PLASMID genetics , *SWINE farms , *ANTI-infective agents , *VETERINARY immunology - Abstract
Highlights • P. multocida isolates with high sulfonamide/trimethoprim MICs were detected in 3/29 farms. • These P. multocida isolates were related in their ApaI and SmaI PFGE patterns. • All of them harboured a plasmid of 6050 bp that was closely related to a plasmid of A. pleuropneumoniae. • The plasmid carried intact sul2 and dfrA14 genes, the latter was integrated into a strA gene. Abstract Pasteurella multocida is an important respiratory tract pathogen in intensive livestock farming, especially in pigs. Antimicrobial agents are frequently used to combat infections caused by this pathogen. In a study on antimicrobial resistance among respiratory tract pathogens of pigs from 30 German pig-producing farms, P. multocida isolates (n = 9) with high minimal inhibitory concentration (MIC) values of 16/304 mg/L (n = 2), 32/608 mg/L (n = 3) or ≥64/1216 mg/L (n = 4) for trimethoprim/sulfamethoxazole (1:19) and of ≥512 mg/L (n = 9) for trimethoprim (TMP) were detected in three of these farms. The genetic relatedness of the isolates was investigated via capsule-specific PCR and macrorestriction analyses with Apa I and Sma I. Pulsed-field gel electrophoresis revealed indistinguishable restriction patterns per farm, with slight differences between the three farms. All isolates represented capsular type A. Four representative isolates, that were subjected to whole genome sequencing, shared the multi-locus sequence type (ST) 3. Their plasmids were transformed into E. coli TOP10 with subsequent selection on TMP-containing agar plates. Antimicrobial susceptibility testing and plasmid analysis of the transformants confirmed that they were resistant to sulfonamides and trimethoprim and carried only a single small plasmid. This plasmid was completely sequenced and revealed a size of 6050 bp. Sequence analyses identified the presence of a resistance gene cluster comprising the genes sul2-ΔstrA-dfrA14-ΔstrA-ΔstrB. Further analysis identified a dfrA14 gene cassette being integrated into the strA reading frame. Neither the gene dfrA14 nor this gene cluster have been detected before in P. multocida. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
24. Novel variant strains of infectious bursal disease virus isolated in China.
- Author
-
Fan, Linjin, Wu, Tiantian, Hussain, Altaf, Gao, Yulong, Zeng, Xianying, Wang, Yulong, Gao, Li, Li, Kai, Wang, Yongqiang, Liu, Changjun, Cui, Hongyu, Pan, Qing, Zhang, Yanping, Liu, Yufeng, He, Hongjiang, Wang, Xiaomei, and Qi, Xiaole
- Subjects
- *
INFECTIOUS bursal disease virus , *ANIMAL experimentation , *IMMUNOSUPPRESSION , *DWARFISM , *VETERINARY immunology - Abstract
Highlights • Novel variant IBDVs were first identified in China.. • The Chinese novel variant IBDVs were obviously different from the American variant IBDV.. • The serious threat of the variant IBDV to chicken was further confirmed by animal experiments. Abstract Infectious bursal disease (IBD) is one of the most important immunosuppressive diseases that seriously threaten poultry farming and food safety worldwide. The variant strain of infectious bursal disease virus (IBDV) has been greatly neglected for more than 30 years. Recently, the subclinical infection of suspected IBD, causing considerable economic losses, occurred in the main chicken-farming regions of China. Through RT-PCR, sequencing, and phylogenic analyses, novel variant IBDVs were first identified in six provinces of eastern China. Immunological detection further confirmed the antigenic variation of the Chinese variant IBDVs. The Chinese IBDV variants were obviously different from the American IBDV variants, with less than a 97.7% (VP1) or 98.7% (VP2) amino acid sequence identity. Animal experiments further confirmed the serious threat of the variant IBDVs to chickens, demonstrating irreversible damage to the central immune organ, obvious immunosuppression, and growth retardation. This study not only identified the pandemic nature of the novel variant IBDVs for the first time but also discovered the distinct molecular epidemiological characteristics of these viruses, which will contribute more to the control of the disease. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
25. Chicken anaemia virus enhances and prolongs subsequent avian influenza (H9N2) and infectious bronchitis viral infections.
- Author
-
Erfan, Ahmed M., Selim, Abdullah A., Helmy, Shawky A., Eriksson, Per, and Naguib, Mahmoud M.
- Subjects
- *
AVIAN infectious bronchitis , *AVIAN influenza , *ANEMIA , *OROPHARYNX , *IMMUNOSUPPRESSION , *VETERINARY immunology - Abstract
Highlights • Chicken anaemia virus infection influences the course of consecutive infection with LPAI H9N2 and IB viruses. • Higher titres of LPAI-H9N2 or IB viruses have been detected in the oropharyngeal swabs, tracheas and kidneys in CAV previously infected chickens. • Pre-infection with CAV directly correlates with elevated levels of IL-6 (in lung) and IFNγ (in lung and spleen). Abstract Immunosuppressive viral diseases have a great economic importance in the poultry industry due to the increased susceptibility to secondary infections. Chicken anaemia virus (CAV) is one of the major immunosuppressive diseases in chickens. In addition, low pathogenic avian influenza (LPAI) of subtype H9N2 and infectious bronchitis (IB) viruses are among the most frequently reported respiratory viral diseases in poultry worldwide. In the present study, specific pathogen free chickens were used to understand the impact of CAV on secondary infection with LPAI-H9N2 or IB viruses. Clinical outcomes, viral shedding dynamics, and cytokine levels wereassessed. The results exhibit that chickens previously infected with CAV produceconsiderablyhigher titresof LPAI-H9N2 or IB viruses in the oropharyngeal swabs (P < 0.05), tracheas and kidneys. In addition, the immunologic effect of CAV provokedthe development of clinical signs of LPAI-H9N2 and IB virus infections. Moreover, results suggested that pre-infection with CAV directly correlated with elevated levels of IL-6 and IFNγ. These findings underline the importance of CAV pre-infection on LPAI-H9N2 or IB infection in chickens, and indicate that co-circulation of CAV can contribute to the spread and evolution of LPAI H9N2 and IB viruses. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
26. Insights into the intestine immune of Marsupenaeus japonicus under the white spot syndrome virus challenge using RNA sequencing.
- Author
-
Hui, Kaimin, Ren, Qian, and Cao, Jun
- Subjects
- *
PENAEUS japonicus , *WHITE spot syndrome virus , *RNA sequencing , *INTESTINAL immunology , *VETERINARY immunology - Abstract
Highlights • The intestine organ transcriptome of kuruma shrimp were constructed. • 63,458 and 44,350 unigenes were obtained from the mock and the WSSV infection sample, respectively. • 12,920 DGEs were identified. • Some immune genes were analyzed. Abstract Intestine is not only the nutrients digestion and absorption centers, but also an important place of microbial infection. Therefore, intestine immunity plays a key defense means for the host against the invasion of pathogenic microorganisms. In this study, we use kuruma shrimp (Marsupenaeus japonicus) as a model to study the intestine immune characteristics of shrimp against WSSV through next-generation sequencing technique. A total of 63,458 and 44,350 unigenes were generated from the control sample and the WSSV infection sample, respectively. Based on homology searches, KEGG, GO, and COG analysis, 39,520 unigenes were annotated. Among them, 12,920 differentially expressed genes were identified. Some of them, including mucin, peritrophin, chitinase, et al., are involved in immune response. These results contribute to a better understanding of the intestine immune response of the shrimp to WSSV. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
27. Truncated chicken MDA5 enhances the immune response to inactivated NDV vaccine.
- Author
-
Han, Qingsong, Gao, Xiaolong, Chu, Zhili, Wang, Xiangwei, Eisa Addoma Adam, Fathalrhman, Zhang, Shuxia, Jia, Yanqing, Qiu, Xinxin, Wang, Xinglong, and Yang, Zengqi
- Subjects
- *
NEWCASTLE disease vaccines , *IMMUNE response , *ADENOVIRUSES , *VETERINARY immunology , *IMMUNOLOGIC diseases in animals - Abstract
Highlights • The property of chMDA5(483aa) used as adjuvant was investigated. • ChMDA5(483aa) delivered by adenovirus enhanced chicken humoral and cell-mediated immune response to inactivated NDV vaccine. • ChMDA5(483aa) expressed by adenovirus improved the protective efficacy of inactivated NDV vaccine. • It gained a new method to strengthen the immune efficacy of NDV inactivated vaccine. Abstract Melanoma Differentiation-Associated protein 5 (MDA5) is a cytoplasmic sensor for viral invasion and plays an important role in regulation of the immune response against Newcastle disease virus (NDV) in chickens. MDA5 was used as an adjuvant to enhance the humoral immune response against influenza virus. In the current study, truncated chicken MDA5 [1–483 aa, chMDA5(483aa)] expressed by recombinant adenovirus was administered to specific-pathogen-free (SPF) chickens to improve the immune response induced by inactivated NDV vaccine. A total of 156 SPF chickens were divided into six groups, and after two rounds of immunization, the humoral immune response, cell-mediated immune (CMI) response and the protective efficacy of the vaccines against NDV challenge were evaluated. The results showed that co-administration of chMDA5(483aa) expressed by adenovirus increased the NDV-specific antibody response by 1.7 times and chickens received chMDA5(483aa) also gained a higher level of CMI response. Consistently, the protective efficacy of the inactivated NDV vaccine against virulent NDV (vNDV) challenge was improved by co-administrate with chMDA5(483aa), as indicated by the reduced morbidity and pathological lesions, lower levels of viral load in organs and reduced virus shedding. Our study demonstrated that chMDA5(433aa) expressed by adenovirus could enhance the immune efficacy of inactivated NDV vaccine in chickens and could be a potential adjuvant candidate in developing chicken NDV vaccines. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
28. The epidemiology of ulcerative mycosis of the platypus (Ornithorhynchus anatinus
- Author
-
Stewart, Niall
- Subjects
Aquaculture ,Veterinary immunology - Abstract
Platypuses ( Ornithorhynchus anatinus) in the north of the island state of Tasmania suffer from a serious disease called ulcerative mycosis, which is responsible for high morbidity and, presumably, mortality rates in areas where the disease occurs. The disease is caused by the fungus Mucor amphibiorum, which is also found in Queensland and New South. However, it does not cause disease in platypuses in those states. This thesis reports on the results of an integrated study of the epidemiology of the disease aimed at determining why only northern Tasmanian platypuses are affected. Pathogenicity trials involving various cane toads (Bufo marinus) as the experimental model indicated that Tasmanian, platypus-derived isolates of M. amphibiorum were more likely to cause a serious, long-term infection than Queensland or West Australian toad-derived isolates. Also, analysis of DNA sequences from four Tasmanian, platypusderived isolates of M. amphibiorum, two Queensland toad-derived isolates and one West Australian slender tree frog (Litoria adelensis)-derived isolate suggest that the area sequenced is not highly variable between isolates from different areas. Based on the DNA analysis and pathogenicity trials, the isolates could be split into three groups; firstly, Tasmanian, platypus-derived isolates, secondly, West Australian slender tree frog-derived isolates and Queensland cane-toad (+) mating type isolates, and thirdly the Queensland cane toad-derived (-) mating type. A closely related fungus, Mucor circinelloides, was also sequenced and tested in pathogenicity trials to determine if it was implicated in the disease. The results suggest that its isolation from diseased animals represents accidental environmental contamination. Sampling of platypus tail fat for the immunomodulating xenobiotics PCB, DDT and Lindane from animals within and outside the endemic area for ulcerative mycosis showed equal levels in all areas. Ulcerated animals did not have elevated levels in comparison to healthy animals. However, some animals from both enedemic and nonendemic areas had greatly elevated levels of PCBs, which may impact on other areas of platypus health. Lymphocyte proliferation assays developed for the platypus showed a low response to mitogens in comparison to most other mammals, but there was no difference between responses for animals from endemic and non-endemic areas. A lowered response to some mitogens was noted in ulcerated animals. A Western blot was developed that could detect antibodies to M. amphibiorum in animals with both active and healed ulcers, but not in healthy platypuses. The possibility that Tasmania possesses a sub-population of atopic platypuses was investigated with an ELISA for the detection of M. amphibiorum specific lgE. There are a number of hypotheses why ulcerative mycosis occurs in Tasmanian, but not mainland, platypuses. The fungus M. amphibiorum may have been introduced into the state, exposing a previously naive population. Tasmanian platypuses, having been isolated from mainland Australia for at least 12,000 years, may be genetically distinct and inherently susceptible to the disease. It is possible that the Tasmanian platypuses' immune system is suppressed, either genetically or by some environmental contaminant. Alternatively, the fungus M. amphibiorum in Tasmania represents a more pathogenic strain than that found in mainland Australia. This thesis will argue that the fungus M. amphibiorum has been introduced into the state on numerous occasions, but in most instances these introductions did not result in infections of platypuses. However, prior to 1982, an isolate of M. amphibiorum successfully infected a platypus, probably because of its ability to produce infective bodies at a much faster rate than other isolates, and the inherent susceptibility of Tasmanian platypuses. Also, some Tasmanian platypuses have suppressed T cell function, which ultimately results in the death of such ulcerated animals.
- Published
- 2023
- Full Text
- View/download PDF
29. Non-coding RNAs in ovine immunity: Identification of unannotated genes and functional analyses of high throughput genomic data
- Author
-
Bilbao Arribas, Martín and Jugo Orrantia, Begoña Marina
- Subjects
molecular biology ,veterinary genetics ,veterinary immunology ,genética veterinaria ,inmunología veterinaria ,biología molecular - Abstract
210 p. Non-coding RNAs (ncRNAs) are involved in several biological processes in mammals, including the immune system response to pathogens and vaccines. The annotation and functional characterization of two of the main classes of ncRNAs, microRNAs (miRNAs) and long non-coding RNAs (lncRNAs), is more advanced in humans than in livestock species, and thus, there is limited knowledge about the function of these transcripts. The main objective of this work was the identification of ovine non-coding genes, concretely miRNA and lncRNA genes, that are involved in the innate and adaptive immune responses induced by vaccines, vaccine components and pathogen infections. For this purpose, high-throughput transcriptome sequencing datasets produced for this purpose and datasets publicly available were analysed with bioinformatic tools and workflows in order to identify unannotated non-coding genes, profile their expression in different tissues and perform evolutionary conservation analyses. More than 12000 unannotated ovine lncRNAs and 1000 ovine miRNAs were identified in the different analyses, with varying levels of sequence conservation. Differential expression analyses between unstimulated samples and samples stimulated with pathogen infection or vaccination resulted in hundreds of lncRNAs and miRNAs with changed expression. Gene co-expression analyses revealed immune gene-enriched clusters associated with immune system activation. These genes make up a prioritized set of potential candidates for deeper experimental analyses. Taken together, these results should help completing the sheep non-coding gene catalogue, and most importantly, they give evidence of immune state-specific ncRNA expression patterns in a livestock species.
- Published
- 2022
30. C-Type Lectins in Veterinary Species: Recent Advancements and Applications
- Author
-
Dimitri Leonid Lindenwald and Bernd Lepenies
- Subjects
C-type lectin ,glycans ,immune modulation ,comparative immunology ,veterinary immunology ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
C-type lectins (CTLs), a superfamily of glycan-binding receptors, play a pivotal role in the host defense against pathogens and the maintenance of immune homeostasis of higher animals and humans. CTLs in innate immunity serve as pattern recognition receptors and often bind to glycan structures in damage- and pathogen-associated molecular patterns. While CTLs are found throughout the whole animal kingdom, their ligand specificities and downstream signaling have mainly been studied in humans and in model organisms such as mice. In this review, recent advancements in CTL research in veterinary species as well as potential applications of CTL targeting in veterinary medicine are outlined.
- Published
- 2020
- Full Text
- View/download PDF
31. Veterinary Immunology -- A Historical Perspective.
- Author
-
Gershwin, Laurel J.
- Subjects
- *
VETERINARY immunology , *MEDICAL societies , *PATHOLOGY , *EQUINE infectious anemia , *NOBEL Prize in Physiology or Medicine , *DOG bites - Abstract
The article focuses on history of veterinary immunology follows closely the history of vaccinology. Topics include the protect animals from infectious diseases has been inoculation with attenuated versions, the pathogen has discovered to afford some degree of protection from field challenge, and the challenge with fresh cultures of the organism, the chickens failed to become infected.
- Published
- 2020
32. Inmunología veterinaria
- Author
-
Gutiérrez Pabello, José Ángel and Gutiérrez Pabello, José Ángel
- Subjects
- Veterinary immunology
- Published
- 2010
33. Veterinary Immunology and Immunopathology
- Author
-
Meier, Sophie, Neumann, Leon, Meier, Sophie, and Neumann, Leon
- Subjects
- Neuroimmunology, Immunopathology, Veterinary immunology
- Abstract
Brucellosis is one of the most important zoonotic diseases worldwide. This book focuses on host natural resistance and innate immunity against Brucella infection. The impact of Johne's disease, a chronic, granulomatous enteritis of ruminants and some wild-type species is examined as well. In addition, the molecular and cellular mechanisms of DNA vaccines are addressed in this book, as well as the optimization strategies that are currently pursued to harness the therapeutic and commercial advantages of DNA vaccination. Moreover, immune responses may substantially differ between conventional laboratory antigens and microbial ones. This book focuses on the experimental pulmonary tuberculosis as a convenient in vivo model to study the disease, providing important advantages over other models. Other chapters examine the defense mechanisms of several flatfish species, which is a main priority to prevent economic losses. The role of the intestine in the immune response in all vertebrates is examined as well, including its cellular components.
- Published
- 2010
34. Veterinary Immunology : Principles and Practice, Second Edition
- Author
-
Michael J. Day, Ronald D. Schultz, Michael J. Day, and Ronald D. Schultz
- Subjects
- Veterinary immunology
- Abstract
Veterinary Immunology: Principles and Practice has become the adopted text in numerous veterinary schools throughout the world. Widely updated with advances in knowledge since 2011, this second edition reflects the rapid development in the field. The new edition presents expanded information on commonly used diagnostic test procedures and discusses
- Published
- 2010
35. Veterinary Immunology : Principles and Practice
- Author
-
Day, Michael J., Schultz, Ronald D., Day, Michael J., and Schultz, Ronald D.
- Subjects
- Veterinary immunology
- Abstract
This book accompanies and complements the teaching of immunology within the veterinary curriculum. It covers large and small animals, and provides clear learning objectives focusing on immunological principles while applying them to the disease process and to clinical practice. The book is case based and illustrated throughout in color.
- Published
- 2010
36. Gene expression and immunolocalization of low‐affinity neurotrophin receptor (p75) in rabbit male reproductive tract during sexual maturation.
- Author
-
Sanchez‐Rodriguez, Ana, Arias‐Alvarez, Maria, Rebollar, Pilar G., Bautista, Jose M., Lorenzo, Pedro L., and Garcia‐Garcia, Rosa M.
- Subjects
- *
NEUROTROPHIN receptors , *GENE expression , *RABBIT reproduction , *VETERINARY immunology , *MALE reproductive organs , *ANIMAL sexual behavior , *MAMMALS - Abstract
Contents: This study reports the gene expression and immunolocalization of the low‐affinity neurotrophin receptor, p75, in accessory glands (prostate and bulbourethral glands) and epididymis (caput and cauda) of male rabbits during sexual maturation. We showed that p75 was expressed in all tissues studied with similar mRNA levels during this period. However, it was differentially immunolocalized in bulbourethral glands and stereocilia of epididymis. These findings may be related with some remodeling processes in the accessory glands during sexual maturation, which could be associated with sperm maturation. The interaction of p75 with neurotrophins in rabbit male reproductive tract suggests the possible implication of this system in sexual maturation in rabbits. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
37. Reimmunization increases contraceptive effectiveness of gonadotropin-releasing hormone vaccine (GonaCon-Equine) in free-ranging horses (Equus caballus): Limitations and side effects.
- Author
-
Baker, Dan L., Powers, Jenny G., Ransom, Jason I., McCann, Blake E., Oehler, Michael W., Bruemmer, Jason E., Galloway, Nathan L., Eckery, Douglas C., and Nett, Terry M.
- Subjects
- *
GONADOTROPIN releasing hormone , *IMMUNIZATION , *HORSES , *VETERINARY vaccines , *VETERINARY immunology , *VACCINATION - Abstract
Wildlife and humans are increasingly competing for resources worldwide, and a diverse, innovative, and effective set of management tools is needed. Controlling abundance of wildlife species that are simultaneously protected, abundant, competitive for resources, and in conflict with some stakeholders but beloved by others, is a daunting challenge. Free-ranging horses (Equus caballus) present such a conundrum and managers struggle for effective tools for regulating their abundance. Controlling reproduction of female horses presents a potential alternative. During 2009–2017, we determined the long-term effectiveness of GnRH vaccine (GonaCon-Equine) both as a single immunization and subsequent reimmunization on reproduction and side effects in free-ranging horses. At a scheduled management roundup in 2009, we randomly assigned 57 adult mares to either a GonaCon-Equine treatment group (n = 29) or a saline control group (n = 28). In a second roundup in 2013, we administered a booster vaccination to these same mares. We used annual ground observations to estimate foaling proportions, social behaviors, body condition, and injection site reactions. We found this vaccine to be safe for pregnant females and neonates, with no overt deleterious behavioral side effects during the breeding season. The proportion of treated mares that foaled following a single vaccination was lower than that for control mares for the second (P = 0.03) and third (P = 0.08) post-treatment foaling seasons but was similar (P = 0.67) to untreated mares for the fourth season, demonstrating reversibility of the primary vaccine treatment. After two vaccinations, however, the proportion of females giving birth was lower (P <0.001) than that for control mares for three consecutive years and ranged from 0.0–0.16. The only detectable adverse side effect of vaccination was intramuscular swelling at the vaccination site. Regardless of vaccine treatment (primary/secondary), approximately 62% (34/55) of immunized mares revealed a visible reaction at the vaccine injection site. However, none of these mares displayed any evidence of lameness, altered gait or abnormal range of movement throughout the 8 years they were observed in this study. Our research suggests that practical application of this vaccine in feral horses will require an initial inoculation that may provide only modest suppression of fertility followed by reimmunization that together could result in greater reduction in population growth rates over time. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
38. Antibody response to Influenza booster vaccination in Franches‐Montagnes stallions supplemented with Equi‐Strath®: a randomized trial.
- Author
-
van Dorland, Hendrika A., Zanoni, Reto, Gerber, Vinzenz, Jeannerat, Elise, Wiederkehr, Danja, and Burger, Dominik
- Subjects
- *
ANTIBODY formation , *INFLUENZA vaccines , *STALLIONS , *BLOOD agglutination , *VETERINARY immunology , *RANDOMIZED controlled trials , *DISEASES - Abstract
Abstract: Bio‐Strath® is a plasmolyzed yeast product enriched with herbs, malt, honey and orange juice. In this study, the effect of Equi‐Strath®, the adapted product for horses, on the equine immune system was evaluated. A routine influenza booster vaccination was used as a model to study the effects of Equi‐Strath® supplementation on the immune response. Twenty healthy Franches‐Montagnes stallions with pre‐existing antibody levels were randomly divided into a study group (SG, n = 10) receiving 0.06 mL/kg bodyweight of Equi‐Strath®, and a control group (CG, n = 10), receiving the same amount of placebo, daily. The supplement and placebo were given from week 1 until week 14 of the trial. After 10 weeks, the horses were vaccinated with a commercial vaccine containing equine influenza strains of the H3N8 subtype. Antibody titres in blood were measured at day 0 before vaccination, and 14 and 32 days after vaccination. In addition, a complete blood count (CBC) was performed on day 0 and day 32. A linear increase of haemagglutination inhibition titres in both groups was observed after vaccination, but with no difference between treatment groups. CBC components remained unaffected by treatment. In conclusion, daily Equi‐Strath® supplementation did not affect the adaptive immune response in stallions after a routine commercial H3N8 influenza booster vaccination. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
39. B Cell Tetramer Development for Veterinary Vaccinology.
- Author
-
Rahe, Michael C., Gustafson, Kevin L., and Murtaugh, Michael P.
- Subjects
- *
VETERINARY vaccines , *DRUG development , *B cells , *TETRAMERS (Oligomers) , *VETERINARY immunology , *PORCINE reproductive & respiratory syndrome , *VACCINATION - Abstract
Immunological memory is elicited after either vaccination or natural exposure to a pathogen and is essential for protection against re-exposure. Despite its critical importance, the ability to interrogate the veterinary animal memory immune response has long been hindered by a paucity of tools to assess immunological memory. As a result, the evaluation and analysis of protective immune responses that predict immune protection in food and fiber animals and facilitate vaccine development are obstructed. To fill this gap in knowledge in swine, we created a B cell tetramer to porcine reproductive and respiratory syndrome virus (PRRSV) nonstructural protein 7 (nsp7) to efficiently and effectively investigate the memory B cell response, a hallmark of anti-viral immunity. This novel reagent was validated by using a modified capture ELISA, tetramer pulldowns, and flow cytometry, and it was shown to detect rare, antigen-specific B cells that were present at a frequency of about 0.001% of total B lymphocytes in immune animals. The nsp7-B cell tetramer will help to characterize the PRRSV-specific memory B cell response, which is fundamentally important for understanding immunological competence and animal variation in resistance to PRRSV infection. We expect that the method will be widely applicable to the exploration of immunity to veterinary pathogens. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
40. Immune Function in Critically Ill Dogs.
- Author
-
Hoffman, D., Amorim, J., and DeClue, A.
- Subjects
- *
IMMUNE response , *DOG physiology , *CRITICALLY ill , *IMMUNOLOGIC diseases in animals , *VETERINARY immunology , *PHAGOCYTOSIS , *CYTOKINES , *PHYSIOLOGY - Abstract
Background: People with critical illness (CI) commonly develop various forms of immune dysfunction, however, there is limited information concerning immune dysfunction in dogs with CI. Hypothesis: The immune response in CI dogs differs from that of healthy dogs. Animals: Immunologic variables were compared between 14 dogs with CI, defined as APPLEfast score of >20 points, admitted to the University of Missouri Veterinary Health Center Small Animal Clinic Intensive Care Unit and healthy controls (n = 15). Methods: Cohort study evaluating constitutive and lipopolysaccharide (LPS)‐stimulated TNF‐α, IL‐6, and IL‐10 production, phagocytosis of opsonized
E. coli and respiratory burst capacity after opsonizedE. coli or phorbol 12‐myristate 13‐acetate (PMA) stimulation, peripheral blood lymphocyte phenotype, and monocyte expressions of HLA‐DR and TLR‐4. Results: Lipopolysaccharide‐stimulated leukocyte TNF‐α (median, Q1, Q3; CI, 49, 49, 120; control, 655, 446, 1174 pg/mL;P = < 0.001), IL‐6 (median, Q1, Q3; CI, 49, 49, 64; control, 100, 49, 166 pg/mL;P = 0.029), and IL‐10 (CI, 49, 49, 56; control, 96, 49, 203 pg/mL;P = 0.014) production and bothE. coli (median, Q1, Q3; CI, 60.5, 43, 88.5; control, 86.6, 81, 89.2%;P = 0.047) and PMA (CI, 40, 11.7, 70; control, 93, 83, 97.6%;P = < 0.001)‐stimulated respiratory burst capacity significantly decreased in CI dogs. Percentage of monocytes expressing TLR‐4 greater in the CI dogs (median, Q1, Q3; CI, 46.9, 24.3, 64.2; control, 16.4, 9.4, 26.2%;P = 0.005). Conclusion: These findings suggest dogs with CI develop immune system alterations that result in reduced respiratory burst function and cytokine production despite upregulation of TLR‐4. [ABSTRACT FROM AUTHOR]- Published
- 2018
- Full Text
- View/download PDF
41. Performance and carcass characteristics of immunocastrated and surgically castrated Iberian pigs fed diets of different protein concentration. Preliminary results.
- Author
-
Seiquer, I., Palma-Granados, P., Lachica, M., Lara, L., Fernández-Fígares, I., Haro, A., and Nieto, R.
- Subjects
- *
SWINE carcasses , *ANIMAL feeding behavior , *VETERINARY immunology - Abstract
Immunocastration has proved to be an effective strategy to prevent sexual development and boar taint in pigs. Previous results indicate that immunocastrated (IC) pigs exhibit better performance than surgically castrated (SC) pigs. In addition to animal welfare benefits, this fact could be of interest for Iberian pigs, as their growth capacity rapidly diminishes as animal ages. The purpose of this study was to examine the effects of immunocastration on Iberian pig performance, protein utilization, and carcass traits under different dietary protein concentrations. Pure Iberian pigs (n=27) were used as follows: 3 sexes (IC males, IC females, SC males); 3 isoenergetic diets (160, 140 and 120 g CP/kg DM); 3 pigs/group. Pigs were vaccinated against Gonadotropin Releasing Hormone at 4.3 months (40 kg) and 6 months (70-80 kg) of age. Experimental diets were offered to individually-housed pigs from 40 to 100 kg BW. Digestibility and N-balance assays were performed at 50 and 90 kg BW, respectively. Pigs were slaughtered at 100 kg BW. Preliminary results indicate greater growth rate for IC males (803 vs 706 and 696 g/d, for IC males, SC males and IC females, respectively; P < 0.001). Feed efficiency (G:F) was greater for IC males (0.289 vs 0.260 and 0.263; P < 0.01). Moreover, IC males exhibited increased carcass length and greater relative weights for most lean cuts, but they also had smaller carcass yield and belly proportions than the rest of groups. No relevant differences related to dietary treatments were detected. Further research would help to elucidate possible effects of immunocastration on Iberian pig lean growth. [ABSTRACT FROM AUTHOR]
- Published
- 2018
42. Adaptation of immunocastration treatment to montanera system for male Iberian pigs: effects on reproductive organs and carcass traits.
- Author
-
Hernández-García, F. I., Izquierdo, M., Del Rosario, A. I., Montero, A., Pérez, M. A., García-Gudiño, J., and Garrido, N.
- Subjects
- *
VETERINARY immunology , *MAMMAL reproduction , *SWINE , *SWINE carcasses - Abstract
Voluntary end of male pig castration is expected in the EU in the near future, but standard immunocastration (IC) protocols need to be adapted for Iberian (IB) pig males due to their long life cycle. Our team developed a 3-dose protocol that needs a further adjustment to suit montanera (MT; free-range acorn feeding) chronology. Our hypothesis is that improving homogeneity of body condition at the start of MT will enhance and homogenize testicular atrophy. Pure IB males (n=35) reared in extensive system were feed-restricted during the growth phase (pre-montanera) as usual. Of these animals, Control pigs (C; n=18) were immunized against GnRH at 10.5, 12 and 13.5 months (m)of age. Treated pigs (T; n=17) were immunized at 10.5, 11.5 and 13 m, with a 15-day ad libitum (AL) feeding period starting at the 3rd dose. Both groups started the MT period at 13.5 m, coinciding with the 3rd dose of C and the end of the AL period of T, and both were slaughtered at 16 m. To further validate the effect of nutritional level, an additional group was fed ad libitum during growth and finishing phases with commercial feedstuff in a regular outdoor rearing system (Adlib; n=15 IB x Duroc males), with the vaccinations taking place at 8, 9 and 11 m and slaughter at 13 m (earlier, due to genotype and unrestricted feeding). Testes and epidydimides were significantly smaller in Adlib and T than C pigs. Similarly, bulbourethral glands were significantly smaller in T than C pigs. Testes parenchyme color "a" (green to red) was highly correlated (r=0.87) with testes weight. Backfat androstenone and skatole were below detection limits for all the animals. Foreleg, loin and prime-cut yields were significantly greater for Adlib than for C and T pigs. In conclusion, nutritional level can be used to improve the efficacy of male IC, and testes colorimetry can be used to monitor this efficacy. [ABSTRACT FROM AUTHOR]
- Published
- 2018
43. Factors influencing the outcome of primary immunization against rabies in young dogs.
- Author
-
Tasioudi, Konstantia E., Papatheodorou, Dimos, Iliadou, Peristera, Kostoulas, Polychronis, Gianniou, Maria, Chondrokouki, Eleni, Mangana-Vougiouka, Olga, and Mylonakis, Mathios E.
- Subjects
- *
IMMUNIZATION , *RABIES in dogs , *RABIES vaccines , *VETERINARY serology , *VETERINARY immunology - Abstract
There is currently limited information on the factors influencing the outcome of rabies vaccination in dogs based on the primary immunization schedule. The objective of this study was to investigate whether selected variables (signalment, number of vaccinations, vaccine brand and multivalence, and time interval between the most recent vaccination and blood sampling) were associated with the achievement of an acceptable titer threshold (based on international standards) and with absolute antibody titers in young dogs vaccinated with commercially available vaccines. Serologic data from 662 dogs tested prior to their first annual booster for rabies were retrospectively reviewed. Neutralizing antibody titers were determined using a fluorescent antibody neutralization test. An acceptable titer threshold (≥0.5 IU/ml) was achieved in 86.5% of the dogs. Dogs that had been vaccinated twice had significantly ( P < 0.001) higher antibody titers compared with dogs vaccinated once. The odds of achieving seropositivity and the median absolute antibody titer tended to decrease with increasing time between vaccination and blood sampling. Dogs vaccinated with monovalent vaccines were more likely to achieve an acceptable titer than dogs vaccinated with polyvalent vaccines. Dogs that were vaccinated after 3–6 months of age were more likely to develop higher antibody titers. These results indicate that the administration of two vaccines rather than one vaccine in the primary immunization schedule for rabies, result in a superior vaccination response and may be a more beneficial policy for ensuring pre-exposure prophylaxis and for travel certification of young dogs. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
44. Bartonella, bats and bugs: A review.
- Author
-
Stuckey, Matthew J., Chomel, Bruno B., de Fleurieu, Eloi Claret, Aguilar-Setién, Alvaro, Boulouis, Henri-Jean, and Chang, Chao-chin
- Subjects
- *
BARTONELLA infections in animals , *ECTOPARASITES , *VETERINARY immunology , *VETERINARY epidemiology , *ZOONOSES , *DISEASE prevalence , *INFECTIOUS disease transmission - Abstract
Ecological, immunological, and epidemiological factors enable bats to transmit an increasingly recognized spectrum of zoonotic agents, and bartonellae are among those emerging pathogens identified in bats and their arthropod ectoparasites. Current data reveal a multifaceted disease ecology where diverse host species distributed around the world interact with a number of Bartonella spp. and several potential vectors. This review summarizes the methods and findings of studies conducted since 2005 to illustrate that Bartonella bacteremia varies by bat species, location, and other potential variables, such as diet with a very high prevalence in hematophagous bats. Among bat families, Bartonella prevalence ranged from 7.3% among Nycteridae to 54.4% in Miniopteridae. Further research can build on these current data to better determine risk factors associated with Bartonella infection in bat populations and the role of their ectoparasites in transmission. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
45. Effect of bee glue on growth performance and immune response of broiler chickens.
- Author
-
Zafarnejad, Kazem, Afzali, Nazar, and Rajabzadeh, Mostafa
- Subjects
- *
PROPOLIS , *BROILER chickens , *POULTRY weight , *VETERINARY immunology , *FEED utilization efficiency , *PHYSIOLOGY - Abstract
The objective of this experiment was to evaluate the effect of bee glue (BG) on the performance, relative visceral weights, some blood parameters, and immune status of broilers. A total of 200 male Ross 308 broiler chicks were randomly assigned to 5 dietary treatments: basal diet (BD), and basal diet supplemented with 600, 700, 800, and 900 mg kg−1of BG. Each of the 5 diets was fed to 4 replicates of 10 chicks each from 0 to 42 days of age. The results indicated that the highest body weight, average daily gain, average daily feed intake, carcass weight, and carcass yield were observed in broilers offered 800 mg kg−1of BG (P < .05). Inclusion of BG improved the feed conversion ratio compared with the control group (P < .01). The calculated European broiler index and crop percentage were greater for the birds received 800 and 900 mg kg−1of BG compared with that of the control birds (P < .05). Inclusion of 900 mg kg−1of BG significantly increased the relative weight of spleen and bursa, but reduced total triglycerides, cholesterol, LDL, and LDL: HDL ratio than the control group (P < .05). The serum IgG and IgM levels were increased for the birds received 700 to 900 mg kg−1BG (P < .01), and addition of BG to the basal diet significantly increased antibody response against sheep red blood cell at 35 days of age (P < .05). It was concluded that an addition of 800 mg kg−1BG to diet improves economic efficiency possibly by creating miniscule improvement in FCR and promoting immune response of broilers. [ABSTRACT FROM PUBLISHER]
- Published
- 2017
- Full Text
- View/download PDF
46. Deciphering the impact of the mitochondrial negative regulator MCJ on host-microbiota interactions in experimental ulcerative colitis
- Author
-
Abecia Aliende, Leticia, Fullaondo Elordui-Zapaterieche, Asier, Genética, antropología física y fisiología animal, Genetika,antropologia fisikoa eta animalien fisiologia, Peña Cearra, Ainize, Abecia Aliende, Leticia, Fullaondo Elordui-Zapaterieche, Asier, Genética, antropología física y fisiología animal, Genetika,antropologia fisikoa eta animalien fisiologia, and Peña Cearra, Ainize
- Abstract
270 p., La colitis ulcerosa (CU) es un trastorno inflamatorio crónico que afecta al intestino. Aunque la causa es desconocida, es una enfermedad multifactorial que está desencadenada por el sistema inmune, factores medioambientales, variaciones genéticas y comunidad microbiana. En trabajos recientes se ha relacionado la disfunción mitocondrial con la EII. Además, se ha descrito que existe una interacción bidireccional entre las mitocondrias y la microbiota, que parece jugar un papel clave en la enfermedad. Por todo ello, con el fin de reducir la heterogeneidad clínica de los pacientes en este trabajo, hemos utilizado un modelo de ratón deficiente en MCJ (Methylation-controlled J protein), una proteína localizada en la membrana interna de la mitocondria que actúa como un regulador negativo del complejo I de la cadena respiratoria, para estudiar el papel de la disfunción mitocondrial dentro de la CU. La inducción de una colitis aguda en ratones deficientes en MCJ resultó en un fenotipo de enfermedad más grave, como consecuencia de la disfunción mitocondrial y una modificación de la composición microbiana. De hecho, se observó un aumento de la presencia de Ruminococcus gnavus, una especie comúnmente incrementada en la microbiota intestinal de pacientes con EII. Los ratones deficientes en MCJ mostraron en el colon una sobre expresión de Timp3, lo cual condujo a la inhibición de la actividad de TACE que es la enzima encargada de la liberación del Tnf unido a la membrana celular, confirmando los hallazgos descritos previamente en macrófagos derivados de médula ósea. Además, los análisis de expresión génica en pacientes que sufren CU también mostraron niveles reducidos de MCJ y una mayor expresión de TIMP3 en el colon. Nuestros resultados también implican los receptores Fc¿R de los macrófagos intestinales en la patogénesis de la CU y en el fallo a la respuesta a terapias biológicas a causa de la disfunción mitocondrial. Finalmente, la inducción de la colitis crónica demostró que e
- Published
- 2022
47. Mycobacteria at the wildlife-livestock interface of the Basque Country. Contributing to the picture of northern Iberian Peninsula.
- Author
-
Agirregomoskorta Sevilla, Iker, Barral Lahidalga, Marta, Zoología y biología celular animal, Zoologia eta animalia zelulen biologia, Varela Castro, Lucía, Agirregomoskorta Sevilla, Iker, Barral Lahidalga, Marta, Zoología y biología celular animal, Zoologia eta animalia zelulen biologia, and Varela Castro, Lucía
- Abstract
272 p., El género Mycobacterium engloba más de 200 especies de micobacterias que se pueden encontrar tanto en el medio ambiente como en los animales y en el ser humano. Algunos de estos microorganismos pueden causar enfermedades de relevancia médica, veterinaria y socioeconómica, provocando un fuerte impacto tanto en la salud pública como en la sanidad animal. La más estudiada es la tuberculosis, una zoonosis infecciosa causada por el complejo Mycobacterium tuberculosis. Las micobacterias no tuberculosas también son de interés sanitario, ya que entre ellas se encuentran especies importantes por su capacidad patógena y por causar interferencias en el diagnóstico de la tuberculosis. El objetivo de esta tesis doctoral fue estudiar el papel de la fauna silvestre en la epidemiología de la tuberculosis y otras infecciones micobacterianas en el País Vasco, una región de baja prevalencia de tuberculosis bovina, así como describir posibles vías de transmisión de micobacterias entre las especies silvestres y el ganado bovino en esta región. Los resultados obtenidos sugieren que el riesgo de transmisión del complejo M. tuberculosis entre la fauna silvestre y el ganado bovino del País Vasco es bajo, mientras que el riesgo de transmisión de micobacterias no tuberculosas podría ser mayor. La transmisión interespecífica de micobacterias se daría fundamentalmente a partir de interacciones indirectas en los pastos. El jabalí, el tejón, el zorro y el ratón de campo parecen ser las especies silvestres más involucradas en la epidemiología de las micobacteriosis en nuestra zona de estudio, siendo el jabalí la más relevante.
- Published
- 2022
48. Non-coding RNAs in ovine immunity: Identification of unannotated genes and functional analyses of high throughput genomic data
- Author
-
Jugo Orrantia, Begoña Marina, Genética, antropología física y fisiología animal, Genetika,antropologia fisikoa eta animalien fisiologia, Bilbao Arribas, Martín, Jugo Orrantia, Begoña Marina, Genética, antropología física y fisiología animal, Genetika,antropologia fisikoa eta animalien fisiologia, and Bilbao Arribas, Martín
- Abstract
210 p., Non-coding RNAs (ncRNAs) are involved in several biological processes in mammals, including the immune system response to pathogens and vaccines. The annotation and functional characterization of two of the main classes of ncRNAs, microRNAs (miRNAs) and long non-coding RNAs (lncRNAs), is more advanced in humans than in livestock species, and thus, there is limited knowledge about the function of these transcripts. The main objective of this work was the identification of ovine non-coding genes, concretely miRNA and lncRNA genes, that are involved in the innate and adaptive immune responses induced by vaccines, vaccine components and pathogen infections. For this purpose, high-throughput transcriptome sequencing datasets produced for this purpose and datasets publicly available were analysed with bioinformatic tools and workflows in order to identify unannotated non-coding genes, profile their expression in different tissues and perform evolutionary conservation analyses. More than 12000 unannotated ovine lncRNAs and 1000 ovine miRNAs were identified in the different analyses, with varying levels of sequence conservation. Differential expression analyses between unstimulated samples and samples stimulated with pathogen infection or vaccination resulted in hundreds of lncRNAs and miRNAs with changed expression. Gene co-expression analyses revealed immune gene-enriched clusters associated with immune system activation. These genes make up a prioritized set of potential candidates for deeper experimental analyses. Taken together, these results should help completing the sheep non-coding gene catalogue, and most importantly, they give evidence of immune state-specific ncRNA expression patterns in a livestock species.
- Published
- 2022
49. Mycobacteria at the wildlife-livestock interface of the Basque Country. Contributing to the picture of northern Iberian Peninsula
- Author
-
Varela Castro, Lucía, Agirregomoskorta Sevilla, Iker, and Barral Lahidalga, Marta
- Subjects
animal ecology ,veterinary immunology ,ecología animal ,inmunología veterinaria ,microbiología veterinaria ,veterinary microbiology - Abstract
272 p. El género Mycobacterium engloba más de 200 especies de micobacterias que se pueden encontrar tanto en el medio ambiente como en los animales y en el ser humano. Algunos de estos microorganismos pueden causar enfermedades de relevancia médica, veterinaria y socioeconómica, provocando un fuerte impacto tanto en la salud pública como en la sanidad animal. La más estudiada es la tuberculosis, una zoonosis infecciosa causada por el complejo Mycobacterium tuberculosis. Las micobacterias no tuberculosas también son de interés sanitario, ya que entre ellas se encuentran especies importantes por su capacidad patógena y por causar interferencias en el diagnóstico de la tuberculosis. El objetivo de esta tesis doctoral fue estudiar el papel de la fauna silvestre en la epidemiología de la tuberculosis y otras infecciones micobacterianas en el País Vasco, una región de baja prevalencia de tuberculosis bovina, así como describir posibles vías de transmisión de micobacterias entre las especies silvestres y el ganado bovino en esta región. Los resultados obtenidos sugieren que el riesgo de transmisión del complejo M. tuberculosis entre la fauna silvestre y el ganado bovino del País Vasco es bajo, mientras que el riesgo de transmisión de micobacterias no tuberculosas podría ser mayor. La transmisión interespecífica de micobacterias se daría fundamentalmente a partir de interacciones indirectas en los pastos. El jabalí, el tejón, el zorro y el ratón de campo parecen ser las especies silvestres más involucradas en la epidemiología de las micobacteriosis en nuestra zona de estudio, siendo el jabalí la más relevante. Neiker INIA: Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
- Published
- 2022
50. Clinical Immunology of the Dog and Cat
- Author
-
Day, Michael J. and Day, Michael J.
- Subjects
- Cats--Immunology, Veterinary immunology, Dogs--Immunology
- Abstract
'The First Edition of this book (1999) was greeted by critical acclaim, became a bestseller and was translated into many languages. It proved useful for veterinarians in general practice who may have had limited exposure to the subject of immunology in training to help translate theory into clinical practice.The book details the manifestations, diagnosis and treatment of immune-related disease in the dog and cat. It is illustrated throughout in full colour, to show and explain to the reader as clearly as possible the complicated principles of disease and immunodiagnostic tests, supported by clinical cases, gross and histopathology, cytology, haematology, immunohistochemistry and other immunological tests.Since publication of the First Edition many advances have been made and the Second Edition incorporates these changes in focus and on techniques. There is an additional chapter on respiratory and cardiac disease, and given the continued focus of companion animal practitioners on vaccine-related issues, a new chapter is devoted entirely to the subject of vaccinology. The Second Edition includes approximately 200 new photographic images, an updated list of further reading and an extensive glossary of terms.'
- Published
- 2008
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.