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In June 2023 several primary nests of the Asian hornet Vespa velutina nigrithorax Buysson, 1905 (Hymenoptera: Vespidae) were found in Landen, a municipality in the province of Flemish Brabant. Later, during October and November, secondary nests were found 300 m from the primary nests, the larvae and pupae of which were examined microscopically. A part of their anatomical structure, in particular the head, is described. [ABSTRACT FROM AUTHOR]

Sampling nests of yellow jackets, Vespula spp. (Hymenoptera: Vespidae), represent a particular challenge due to the danger involved in working with live colonies of a potentially aggressive venomous super‐organism. Here, we report on sampling methods using a petrol‐driven leaf blower/vacuum and a battery‐powered vacuum to sample returning Vespula germanica (Fabricius) foragers at their nest entrance. These methods make the sampling of returning foragers at the nest more efficient and convenient and represent a low‐risk approach to sampling. Applications include prey analysis and genetic sampling that can be done destructively or non‐destructively depending on the geographic location. We sampled 15 nests using the two‐stroke vacuum apparatus and collected on average (±SD) 454 ± 271 wasps per nest. We believe that the petrol‐driven vacuum collection apparatus we designed is a useful method for collecting a large number of social wasp foragers returning to a single‐nest entrance. [ABSTRACT FROM AUTHOR]

Social insects have developed different nest defence strategies; for example, social wasps use nonaggressive mechanisms or aggressiveness, including stinging. This subject has been widely examined in the literature, and it may help improve our understanding of wasps' ecology. The nesting behaviour of some genera is still poorly known. In this paper, we report new information on the nesting behaviour of the species Clypearia in different Brazilian biomes. We observed six nesting events in natural and anthropic environments of the Amazon rainforest, Atlantic forest, and Cerrado domains, sporadically from 2004 to 2022. From our observations, we suggest that Clypearia nest defence includes (i) preference for nest camouflage rather than aggressive behaviour; (ii) selection of nesting sites close to water sources in natural or anthropic environments, and eventually obtaining food from the substrate; (iii) nesting association with ants for mutual protection against predators; and (iv) variations in the nests' height. Our records suggest nest camouflage and association with ants are strategies for nest defence. These may have influenced nest height in relation to the ground, which may also vary according to the environment explored by the species. [ABSTRACT FROM AUTHOR]

Simple Summary: In recent years, with global warming and climate change, there have been many cases of successful hornet invasions in some areas. The successful invasion of hornets not only has an impact on local human security and economic activities, but also seriously threatens the bee ecosystem, which has attracted the attention of scientists. Therefore, taking some measures to suppress the foraging and expansion of hornets becomes an important subject. Compared with attractant trapping and poison baits, hornet-tracking technology, which tracks hornets to find their nests, is a more efficient and feasible method. This review paper discusses several common hornet-tracking methods, analyzes the advantages and disadvantages of different methods, and provides a theoretical basis for the development of hornet-tracking technology. Hornet is a general term for insects of the genus Vespa (Hymenoptera: Vespidae). Hornets are predatory insects distributed worldwide. They often appear at apiaries in groups to prey on honey bees, and cause incalculable losses in the honey bee industry. In the face of hornet intrusion, tracking a homing hornet to find its nest is the most efficient way to discover and eliminate the hornets around an apiary. Here, five hornet-tracking methods (hornet tag tracking, triangulation, thermal imaging technology, harmonic radar, and radio telemetry) are reviewed. The advantages, disadvantages and feasibility of each method are discussed to improve the strategies for tracking hornets. Therefore, this review provides ideas for the development of hornet-tracking technology and for improving honey bee protection. [ABSTRACT FROM AUTHOR]

Social wasps play an important role in controlling agricultural pests. The present study aimed to investigate the differences in abundance, species richness and composition of social wasps (Vespidae: Polistinae) between a Cerrado legal reserve, edge and agricultural matrix in an agricultural farm in Mato Grosso, Brazil. We delimited three transects and used Malaise traps which remained active for 48 hours, and were distributed equidistant over five distances from the edge to the legal reserve and agricultural matrix. In total, we collected 618 individuals and 31 species of social wasps. Our results showed that social wasp abundance was similar between the legal reserve and agricultural matrix during three stages and species richness only non-cropping stage. A similar pattern was observed for composition similarity, which presented a lower value between the legal reserve and agricultural matrix during the non-cropping stage. Our results indicate that although monoculture areas can offer food resources and be attractive to social wasps during the active cropping stages, these areas cannot host and conserve the diversity of social wasps. Legal reserve areas, for maintaining the diversity and ecosystem services provided by social wasps. [ABSTRACT FROM AUTHOR]

For an ongoing systematic study of Korean Vespidae, we analyzed the complete mitochondrial genome of a social wasp, Vespula rufa (Linnaeus 1758), from the South Korea. The mitogenome is 17,521 bp in length, comprising 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, and two ribosomal RNA (rRNA) genes. The nucleotide composition is 40.5% adenines, 43.0% thymines, 6.0% guanines, and 10.5% cytosines. The GC content is 16.5%. A maximum-likelihood analysis was conducted to understand phylogenetic relationships, based on 13 complete mitogenome sequences of Vespinae species. We recognized that V. rufa is being placed basal within the genus Vespula. The complete mitochondrial genome of V. rufa provides useful genetic information for further studies. [ABSTRACT FROM AUTHOR]

This study delves into the concept of niche conservatism (NC) and its implications for how hornets (Hymenoptera: Vespidae) and honeybees respond to climate change. Our primary objectives are threefold: firstly, to assess whether distinct environmental niche spaces (E‐space) exist between 12 hornets and honeybees like Apis cerana and Apis mellifera in Asia; Secondly, to explore the degree to which Asian hornets have attained geographic equilibrium alongside honeybee species. Lastly, is to investigate how the geographic niche overlap (G‐space) between hornets and honeybees could potentially change under climate change scenarios. To accomplish these goals, we employed ordination and ecological niche modeling techniques to analyze 91 pairs of hornets and honeybees in both geographic (G‐space) and environmental (E‐space) contexts. Then, we projected the potential impacts of climate change on the future geographic overlap between hornets and honeybees, specifically under the SSP585 climate scenario for the year 2070. Our results demonstrated that the environmental niches (E‐space) of hornets and honeybees can be treated as interchangeable, indicating they have similar environmental preferences despite being unrelated taxa. We found that Vespa velutina currently exhibits a moderate geographic niche overlap (G‐space) of 0.63 with both honeybee species. Meanwhile, Vespa mandarinia demonstrates an overlap of 0.46 with Apis cerana and 0.63 with Apis mellifera. The overlap of Vespa velutina with Apis cerana might potentially decrease to 0.51 and 0.56 with Apis mellifera. For Vespa mandarinia, the overlap could reach 0.41 with Apis cerana and 0.6 with Apis mellifera under a climate change scenario. This study indicates that the limited spatial overlap between honeybees and hornets across certain areas in Asia is more likely influenced by geographical barriers rather than solely environmental unsuitability for hornets. In this study, we delve into the concept of niche conservatism (NC) and its implications for how hornets (Hymenoptera: Vespidae) and honeybees respond to climate change. [ABSTRACT FROM AUTHOR]

Simple Summary: Invasive wasps can have huge impacts on global apiculture. Here, we developed 12 range dynamic models to globally calibrate the future range dynamics and invasion hotspots of 12 major invasive Vespidae wasp species through a unified approach and evaluated their future impacts on apiculture worldwide. We detected increases in their habitat suitability in most parts of the globe and substantial range expansions, both mainly driven by future climatic changes. We also identified those invasive wasp species projected to have the largest potential ranges, highest range expansion ratios, and largest centroid shifts, as well as the invasion hotspots of all major invasive wasp species in the future. In summary, the increasing habitat suitability and range dynamics of invasive wasps indicate that global apiculture will likely face greater adverse impacts in the future. Therefore, our study provides important and novel information for combatting invasions of wasps and mitigating their expected impacts on global apiculture. Until now, no study has examined the future range dynamics of major invasive wasp species to assess their future impacts on global apiculture. Here, we developed 12 species distribution models to calibrate the future range dynamics of 12 major invasive Vespidae wasp species under a unified framework. An increase in their habitat suitability was identified in more than 75% of global land. Substantial range expansions were detected for all 12 species, and they were primarily induced by future climate changes. Notably, Polistes dominula and Vespa crabro had the largest potential ranges under all scenarios, suggesting their greater impact on global apiculture. Polistes chinensis and Vespa velutina nigrithorax had the highest range expansion ratios, so they warrant more urgent attention than the other species. Polistes versicolor and P. chinensis are expected to exhibit the largest centroid shifts, suggesting that substantial shifts in prioritizing regions against their invasions should be made. Europe and the eastern part of the USA were future invasion hotspots for all major invasive wasp species, suggesting that apiculture might face more pronounced threats in these regions than in others. In conclusion, given their substantial range shifts, invasive wasps will likely have increasingly negative impacts on global apiculture in the future. [ABSTRACT FROM AUTHOR]

We assess the accuracy of geometric morphometrics (GMM) for determining the origin of insects of quarantine importance using the Asian hornet (Vespa velutina Lep.1836) as a case study. This species is highly variable, has an extensive natural distribution, and has been transported to many regions of the world. Forewing landmarks were applied to a large sample of regionally specific color morphs (previously considered "subspecies") from across the species' native Asian range. We reconfirm that GMM can statistically distinguish geographic variants independent of the color patterns that have heretofore been used for provenance, but which have been suspected of being unreliable. Almost all morphs in our analyses were statistically different except the centrally located V. v. variana, whose range lies between the continental V. v. auraria Smith, 1852, and V. v. nigrithorax du Buysson, 1905 morphs, and the Malaysian and Indonesian morphs. Even with moderate-sized training samples, discriminant function analysis (DFA) was able to classify geographic morphos with about 90% accuracy (ranging from 60% to 100%). We apply these results to determine the origin of a dead wasp recently intercepted in a mail parcel in Utah. Both DFA and continuous-trait maximum-likelihood clustering suggest that the Utah specimen belongs to the nigrithorax morph, which is native to southern China but now invasive in Europe, Japan, and Korea. These results are also supported by DNA barcode analysis, which groups the Utah individual with nigrithorax populations in South Korea and Japan. The relationship between variation in wing shape and genetic differentiation deserves further study, but molecular data are consistent with the GMM results suggesting that morphometric comparisons may be able to identify and provenance intercepted specimens quickly and inexpensively when molecular sequences and taxonomic specialists are unavailable. [ABSTRACT FROM AUTHOR]

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This study examines the diversity of Hymenoptera in Jai Narain Vyas University new campus in Jodhpur, Rajasthan. Analyse their distribution and abundance in different microhabitats. Survey runed over a ten-month period from September 2022 to June 2023. Total 15 species from6 different families (Apidae, Formicidae, Sphecidae, Braconidae, Vespidae, Ichneumonidae) were recorded. The results showed that the diversity of species had different microhabitats, with the highest diversity found in gardens and trees. The results of this study are important for understanding the bee diversity of JNVU and suggesting conservation and management strategies to preserve the rich biodiversity of the region. [ABSTRACT FROM AUTHOR]

In this paper, we present the results of our study on the seasonal dynamics and altitudinal distribution of Vespidae in forest ecosystems of central European Russia. To conduct this study, we utilized beer traps. Our findings include the recording of 32 species from 4 families of Hymenoptera. The most numerous family was Vespidae. The dominant species from this order were Vespula vulgaris, Vespa crabro, Dolichovespula media, and Vespula germanica. The occurrence of these species was quite high, including species from the Crabronidae, Chrysididae, and Pompilidae families. These species are known to frequently visit flowers of various plants and feed on nectar and pollen. In five different biotopes, the highest number of Vespidae was found in oak forests, while the lowest number was observed in pine forests. Vespula vulgaris was the dominant species in birch and oak forests, while Vespa crabro was more prevalent in pine and linden forests. The seasonal dynamics of the number showed a large peak in early July, likely due to a significant increase in air temperature during the day and night. This peak may have been caused by a large number of new-generation workers leaving the nests at this time. Additionally, the number of Vespidae in traps located in the undergrowth was slightly higher than those in traps in the canopy. [ABSTRACT FROM AUTHOR]

For an ongoing systematic study of Korean Vespidae, we analyzed the complete mitochondrial genome of a social wasp, Vespula rufa (Linnaeus 1758), from the South Korea. The mitogenome is 17,521 bp in length, comprising 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, and two ribosomal RNA (rRNA) genes. The nucleotide composition is 40.5% adenines, 43.0% thymines, 6.0% guanines, and 10.5% cytosines. The GC content is 16.5%. A maximum-likelihood analysis was conducted to understand phylogenetic relationships, based on 13 complete mitogenome sequences of Vespinae species. We recognized that V. rufa is being placed basal within the genus Vespula. The complete mitochondrial genome of V. rufa provides useful genetic information for further studies.

For social wasps, the selection of the nesting substrate represents the success or failure of the colony, and temperature is a determining factor for this selection. With the increase in global temperature due to climate change, it is essential to understand how temperature actually interferes in the selection of the foundation site of social wasp colonies. Here, we describe a curious nestfounding strategy on a rocky outcrop in the state of Minas Gerais, Brazil. Social wasps selected the lighter areas of the outcrop where they had greater success in developing their colonies. These lighter areas absorb less heat and had a lower average temperature when compared to darker areas.

Niche evolution refers to the process by which species undergo changes in ecological interactions, as well as their ability to disperse over time. Our study focuses on the widely distributed neotropical genus of social wasps, Synoeca (Hymenoptera, Vespidae, Epiponini). We use ecological niche modeling to investigate the niche evolution of this insects, to explore how species have evolved within and across distinct environmental boundaries, as well as to explore the overlap, equivalence, and similarity between their niches. Our analysis of Predicted Niche Occupancy reveals that species occupy heterogeneous niches in relation to temperature, precipitation, and altitude, similar to the patterns observed in the analysis of the evolutionary history of climate tolerances, which shows that species have evolved to occupy distinct niche ranges. In addition, our niche comparisons indicate that the species do not share similar niches with each other. All these results suggest that Phylogenetic Niche Conservatism may be playing a significant role as a process contributing to the allopatric pattern observed in this genus. This study represents the first investigation of niche evolution in Vespidae, providing valuable insights for future research into the evolutionary dynamics of insects. [ABSTRACT FROM AUTHOR]

Deuterodiscoelius Dalla Torre and Pachycoelius Giordani Soika are two small genera of Australian vespid wasps. While the first came from the splitting of Discoelius, the latter was created to accommodate species with a few distinct traits, some of which were not observable due to the absence of male specimens of two out of the three species designated to the genus. This already blurry delimitation of the genera was heightened when specimens of an unknown species presented a combination of characters from both genera. Hence, to properly assign this species to a genus, a phylogenetic study based on morphology was carried out before the species description. The analysis recovered both genera as unnatural, resulting in the synonymy of Pachycoelius under Deuterodiscoelius, and the new species, namely, Deuterodiscoelius delator Lopes and Carpenter, new species, was subsequently described. [ABSTRACT FROM AUTHOR]

Here, we tested the application of morphological skew in Agelaia timida by reporting caste dimorphism and comparing it with other epiponine species. A description of a nest of A. timida is also provided. Females were measured to investigate morphological differences between castes and dissected to assess ovarian development. Three types of ovaries were found: filamentous (workers), developed with some mature oocytes (intermediates), and developed and inseminated (queens). Queens are bigger than workers, but discriminant analysis showed castes are not morphologically distinct. Mahalanobis distance showed a statistical separation between inseminated and non-inseminated females, and the only distinct group was workers. The results differ from other species of Agelaia, which present clear-cut caste differences. Thus, A. timida fits into morphological skew theory since it forms small colonies with low caste differentiation and nonsterility of workers. These features, plus the presence of a true envelope, may be plesiomorphic, as observed in the ground plan of Epiponini. [ABSTRACT FROM AUTHOR]

Social wasps of the genus Polistes Latreille, 1802 abound in urban environments, where they use diverse anthropic structures to locate their colonies during the late spring and summer period. This direct contact with the population gives rise to a general feeling of fear or rejection towards these vespids due to the possibility of receiving stings. The aim of this study was to reveal the social perception towards these social wasps in a small urban area in the southwest of the Iberian Peninsula. The results showed that 90.44% of 272 respondents reported to have been stung by these species, of which more than 50% admit having suffered five or more attacks in their lifetime. The possible causes of these stings have been classified, as well as the type of reaction by the inhabitants towards the existence of wasps' nests in their domestic environment. Finally, it is important to mention that most of the respondents recognize the biological importance of these hymenopterans, despite the general fear they show towards them. [ABSTRACT FROM AUTHOR]

Two complete nests of Pterocheilus phaleratus (Panzer, 1797) were described from the north-eastern edge of the Pomeranian Flying Club in Toruń (northern Poland). Both nests were built in sandy terrain on a sloping surface (about 20°). The nests consists of a 5 cm long main burrow with a single cell. Digging and backfilling the nest involves carrying fine gravel in the mandibles. Females transported prey at a frequency of 6 to 12 minutes. The cell contained 25 larvae from the Tortricidae family (Acleris spp.). Metopia cf. argyrocephala (Meigen, 1824) (Diptera: Sarcophagidae) was observed following the female into the nest, but specimens of this species were not excavated from the cell. [ABSTRACT FROM AUTHOR]

Simple Summary: Our study examined antennal structures in nine species of Vespinae wasps, providing insights into their morphology and sensilla diversity. Using SEM, we identified 19 sensilla types, including unique variations in pit organs and sensilla trichodea. The males of seven species exhibited tyloids. This research enhances our understanding of sensory systems in Hymenoptera, aiding taxonomy and evolutionary studies. This study investigates the distribution, morphology, and potential functions of antennal sensilla in various wasp species, including Dolichovespula flora, D. intermedia, Vespula structor, Vl. vulgaris, Provespa barthelemyi, Vespa bicolor, V. ducalis, V. mocsaryana, and V. velutina var. nigothorax. The study thoroughly analyzes the antennal structure of these species, representing all four genera of the yellow-jacket and hornet subfamily Vespinae. Using scanning electron microscopy (SEM), the study identifies a total of nineteen types of sensilla, including sensilla trichodea (ST-I, ST-II, ST-III), sensilla campaniform (SCF-I, SCF-II, SCF-III), pit organs (SCO-I, SCO-II, and SA), sensilla placodea (SP-I, SP-II), sensilla chaetica (SCH-I, SCH-II), sensilla basiconica (SB-I, SB-II), sensilla agmon (SAG-I, SAG-II), and sensilla coelocapitular (SCA). Additionally, tyloids were observed in the males of seven species, except for Vl. structor and Vl. vulgaris. The study provides insights into these sensilla types' morphology, abundance, and distribution. It discusses the variations in sensilla morphology among different species and the presence of gender-specific sensilla. This study provides new data about the morphology and distribution patterns of sensilla and tyloid. [ABSTRACT FROM AUTHOR]

The ichneumonid parasitoid Stenarella insidiator (Smith, 1859) is recorded for the first time from Vietnam based on the specimens collected from Tam Dao National Park in Vinh Phuc province, Na Hang district in Tuyen Quang province, and Bidoup-Nui Ba National Park in Lam Dong province. In addition, the wasp Orancistrocerus aterrimus (de Saussure, 1852) (Hymenoptera: Vespidae) is reported as a new host of this parasitoid. The parasitoid is described based on Vietnamese material with information of its overwintering in the wasp nest. [ABSTRACT FROM AUTHOR]

In the Galapagos Islands, invasive wasps negatively affect the local fauna through competition with native predators. These wasps also pose a risk to humans because of their stings. Thus, it is important to constantly survey these insects. Currently there are three species of vespids known from the Galapagos: two social species are invasive, while a solitary species is cryptogenic. After structured sampling using malaise traps, hand nets, and pan traps on most of the islands of the archipelago, and a review of related collections, the distribution of Brachygastra lecheguana is expanded to Genovesa Island; precise localities and vegetation zones are recorded for Polistes versicolor; and updated information on the distribution of the cryptogenic solitary vespid Pachodynerus gaullei is provided. [ABSTRACT FROM AUTHOR]

Introduction: Building on our previous work, we investigate how dietary shifts affect gut microbial essential amino acid (EAA) provisioning in the lactating cockroach Diploptera punctata. Method: To that end, we fed cockroaches three distinct diets: a plant-only Gari diet composed of starchy and granulated root tuber Yucca (Manihot esculenta), a dog food diet (DF), and a cellulose-amended dog food (CADF) diet. We anticipated that the high carbohydrate, low protein Gari would highlight increased microbial EAA supplementation to the host. Results: By day 28, we observed distinct profiles of 14 bacterial families in the insect gut microbiomes of the three dietary groups. CADF-fed insects predominantly harbored cellulolytic and nitrogen-fixing bacteria families Streptococcaceae and Xanthomonadaceae. In contrast, Gari-fed insects were enriched in anaerobic lignocellulolytic bacteria families Paludibacteraceae and Dysgonomonadaceae, while DF-fed insects had a prevalence of proteolytic anaerobes Williamwhitmaniaceae and sulfate-reducing bacteria Desulfovibrionaceae. Furthermore, we confirmed significantly higher EAA supplementation in Gari-fed insects than in non-Gari-fed insects based on Î'13C-EAA offsets between insect and their diets. The Î'13C-EAA offsets between DF and CADF were nearly indistinguishable, highlighting the relevance of using the plant-based Gari in this experiment to unequivocally demonstrate this function in this insect. These results were underscored by lower standard metabolic rate (SMR) relative to the DF insect in Gari-fed (intermediate SMR and dietary quality) and CADF (least SMR and dietary quality) insects. Discussion: The influence of diet on EAA provisioning and SMR responses in insects underscores the need for further exploration into the role of gut microbial functions in modulating metabolic responses. [ABSTRACT FROM AUTHOR]

The springtail Seira tinguira was observed for the first time in the state of Minas Gerais in a colony of the social wasp Polistes ferreri. The colony was collected in the Botanical Garden of the Federal University of Juiz de Fora, a fragment of Montane Seasonal Semideciduous Tropical Forest, located in the urban perimeter of the municipality of Juiz de Fora, southeast of the state of Minas Gerais, Brazil. These springtails were recorded walking on the inner base of the cells of the nest close to the meconium, which served as the substrate for the Aspergillus sp. hyphomycete fungus. There was no removal or defense behavior against the springtail, however, the wasps occasionally showed alarm behavior, characterized by opening the wings, contracting the abdomen, and actively patrolling the nest. [ABSTRACT FROM AUTHOR]

Cytogenetics has allowed the investigation of chromosomal diversity and repetitive genomic content in wasps. In this study, we characterized the karyotype of the social wasp Mischocyttarus cassununga using conventional cytogenetics and chromosomal mapping of repetitive sequences. This study was undertaken to extend our understanding of the genomic organization of repetitive DNA in social wasps and is the first molecular cytogenetic insight into the genus Mischocyttarus. The karyotype of M. cassununga had a chromosome number of 2n = 64 for females and n = 32 for males. Constitutive heterochromatin exhibited three distribution patterns: centromeric and pericentromeric regions along the smaller arms and extending almost the entire chromosome. The major ribosomal DNA sites were located on chromosome pair in females and one chromosome in males. Positive signals for the microsatellite probes (GA)n and (GAG)n were observed in the euchromatic regions of all chromosomes. The microsatellites, (CGG)n, (TAT)n, (TTAGG)n, and (TCAGG)n were not observed in any region of the chromosomes. Our results contrast with those previously obtained for Polybia fastidiosuscula, which showed that the microsatellites (GAG)n, (CGG)n, (TAT)n, (TTAGG)n, and (TCAGG)n are located predominantly in constitutive heterochromatin. This suggests variations in the diversity and chromosomal organization of repetitive sequences in the genomes of social wasps. [ABSTRACT FROM AUTHOR]

The Asian paper wasp (Polistes chinensis) is an invasive species in New Zealand and a voracious arthropod predator, incorporating a wide range of prey into its diet. We examined the colony survival and prey community composition of these wasps in a protected coastal habitat in New Zealand. Paper wasp colonies at this site were surveyed and monitored weekly over two summers. Our data showed that only ~20% of the monitored colonies each year survived until late summer, with high rates of colony mortality in late spring and early summer. We collected samples of wasp larval guts over a temporal gradient in one nesting season, and via DNA metabarcoding analysis, we identified the prey species consumed. The prey species most frequently identified in larval samples were endemic cicadas and several lepidopteran species. No native arthropod species of known conservation concern were identified in the analysis. However, 63% of the unique taxon sequences retrieved could not be identified by genus or species level, likely due to the absence of reference barcodes. These taxa may represent a group of understudied species, potentially highly endemic or localised. Our analysis indicates that these invasive wasps are opportunistic‐generalist predators with the potential to exert high predation pressure on native arthropods. P. chinensis may be preying on a range of understudied species, especially in remote, natural habitats across New Zealand. We recommend future studies continue to barcode native New Zealand arthropods in order to improve the taxonomic assignments of dietary studies. [ABSTRACT FROM AUTHOR]

Udeus Champion, 1902 is a weevil genus in the tribe Eugnomini associated with the inflorescences of Cecropia Loefl. (Urticaceae), with 11 valid species distributed in the Neotropics. Species of Udeus are generally rare in collections and their biology is poorly known, despite a high abundance in nature. Here, we describe Udeus cerradensis Lira, de Medeiros & Grossi, sp. nov. from the Brazilian Cerrado, including the first descriptions of larvae and pupae for a Neotropical eugnomine. We assess their role as potential pollinators of their host plant Cecropia saxatilis Snethl. and find that adults do not visit pistillate flowers and therefore are unlikely to be pollinators. This contrasts with previous reports in a different pair of species in Cecropia‐Udeus, suggesting that pollinator agents vary between species in this plant genus. Finally, we provide notes about the predation of larvae of U. cerradensis by social wasps Synoeca surinama (L.) and Protopolybia aff. sedula (Saussure), revealing that, in this instance, an endophytic larva does not prevent predation by a behaviourally flexible predator. This study is the first of a series on the natural history of Udeus and their specialised interactions with host plants. [ABSTRACT FROM AUTHOR]

Simple Summary: We studied whether the female body size of strepsipteran parasites of Xenos oxyodontes is affected by the following four factors: season, host body size, multiparasitism, and reproductive conditions of the host wasp. The cephalothorax width of female parasites did not change throughout the seasons but was strongly affected by host body size, number of parasites per host wasp, and reproductive conditions of host wasps. Our results suggested that female parasites with larger body sizes have greater potential for reproduction. Parasite growth in hosts depends on their hosts' nutritional status. This study examined whether the body size of the strepsipteran parasite Xenos oxyodontes, which parasitizes the eusocial wasp Vespa analis, is affected by several key factors, including host body size. We collected V. analis using bait traps for three years in Niigata, Japan, and evaluated the number of male and female X. oxyodontes parasites throughout the seasons. A total of 185 female parasites were collected, and their cephalothorax widths were measured. The widths of female parasites did not statistically vary among seasons and were positively correlated with the head widths of female host wasps but negatively correlated with the number of parasites per host wasp. In addition, we examined whether the reproductive conditions of post-hibernation solitary queens affected the cephalothorax width of female parasites. The widths of the female parasites were greater when the queens had less-developed ovaries. These results suggested that nutrient availability by female parasites depends on the host wasp and competition with conspecific individuals. [ABSTRACT FROM AUTHOR]

This study aimed to investigate the venom sac extracts (VSEs) of the European hornet (EH) Vespa crabro (Linnaeus, 1758) (Hymenoptera: Vespidae), focusing on the differences between stinging females, gynes (G), and workers (W), at the protein level. Using a quantitative "Sequential Window Acquisition of all Theoretical Fragment Ion Mass Spectra" (SWATH-MS) analysis, we identified and quantified a total of 240 proteins. Notably, within the group, 45.8% (n = 110) showed significant differential expression between VSE-G and VSE-W. In this set, 57.3% (n = 63) were upregulated and 42.7% (n = 47) downregulated in the G. Additionally, the two-hundred quantified proteins from the class Insecta belong to sixteen different species, six of them to the Hymenoptera/Apidae lineage, comprising seven proteins with known potential allergenicity. Thus, phospholipase A1 (Vesp v 1), phospholipase A1 verutoxin 2b (VT-2b), hyaluronidase A (Vesp v 2A), hyaluronidase B (Vesp v 2B), and venom allergen 5 (Vesp v 5) were significantly downregulated in the G, and vitellogenin (Vesp v 6) was upregulated. Overall, 46% of the VSE proteins showed differential expression, with a majority being upregulated in G. Data are available via ProteomeXchange with identifier PXD047955. These findings shed light on the proteomic differences in VSE between EH castes, potentially contributing to our understanding of their behavior and offering insights for allergy research. [ABSTRACT FROM AUTHOR]

The knowledge of entomopathogenic fungus in social wasps is limited, however, association appears to be more common than past thinking. In this study, we recorded the occurrence of eight social wasp species infected: Agelaia fulvofasciata, Agelaia testacea, Apoica cf. pallida, Polistes rufiventris, Polybia dimidiata, Polybia liliacea, Polybia striata, Synoeca virginea, Polistes (Aphanilopterus), and one Mischocyttarus (Mischocyttarus) morphotypes by Ophiocordyceps sphecocephala for the first, and added some new geographic range extension records for the already reported association of Ophiocordyceps humbertii for some locations in the Neotropics with a brief behavioral diagnosis of the species. [ABSTRACT FROM AUTHOR]

Espécies de vespas sociais do gênero Agelaia (Vespidae, Polistinae) possuem hábito alimentar necrófago, além de predar outros insetos, mas pouco se sabe sobre seu comportamento forrageiro. Portanto, o objetivo deste estudo é documentar o comportamento de forrageamento de Agelaia pallipes (Olivier, 1791) em carcaça de uma cigarra (Hemiptera) presa em uma teia de aranha. A observação ocorreu ocasionalmente no dia 4 de outubro de 2023, no beiral de uma casa em área antropizada, município de Inconfidentes, região Sul de Minas Gerais, Domínio da Mata Atlântica. Foram observados quatro indivíduos de A. pallipes consumindo a carcaça de uma cigarra, presa na teia de aranha Trichonephila clavipes (L., 1767). Entretanto, não está claro se o uso de presas em teias de aranha é um comportamento comum para A. pallipes. Portanto, mais estudos são necessários para investigá-lo mais profundamente. Da mesma forma, são necessários estudos que avaliem o impacto nas aranhas da usurpação de presas em suas teias por vespas sociais.

We investigated the richness and composition of social Hymenoptera collected with a Malaise trap in a rainforest in the Amazon. In addition, we studied the possibility of reducing the number of traps and the time the traps remain in the field to minimize the sampling effort. In reducing sampling effort, we seek to maintain taxonomic standards (similarity in composition) and reduce both the time the Malaise trap remains in the field and the number of traps per plot. We collected 63 species of social Hymenoptera, represented by 11 species of Apidae, 31 of Formicidae, and 21 of Vespidae, in 30 plots of 250 m distributed in 25 km² of rainforest in the Amazon. It was possible to reduce the permanence time of the Malaise trap in the field from 72 to 48 hours without significant loss of the maximum recorded diversity since there is high similarity (80%) to the maximum effort of 72 hours of sampling. The reduction from two to one trap directly impacted the sampled diversity. We estimate savings of 30.53% by reducing sampling effort and trap dwell time, allowing the resources saved to be invested more efficiently in biodiversity monitoring studies in the Amazon basin.

Wasps play important roles as predators and pollinators in the ecosystem. The Jingpo minority residing in Yunnan Province, China, has a traditional practice of using wine infused with mature wasps as a customary remedy for managing rheumatoid arthritis. The larva of the wasp is also a tasteful folk dish that has created a tremendous market. There is a paucity of survival knowledge, which has greatly restricted their potential applications in food and healthcare. Recent research has highlighted the importance of gut microbiota in insect growth. Nevertheless, there is still a lack of understanding regarding the composition, changes, and functions of the gut microbiota in Vespa mandarinia during development. In this research, the gut microbiota were investigated across three growth stages of Vespa mandarinia using a metagenomic technology. The result revealed that there are significant variations in the proportion of main gut microbes during the metamorphosis of Vespa mandarinia. Tenericutes were found to dominate during the larval stage, while Proteobacteria emerged as the dominant group post-pupation. Through a comprehensive analysis of the gut microbiota metagenome, this study revealed functional differences in the wasp gut microbiota at various growth stages. During the larval stage, the gut microbiota plays a central role in promoting metabolism. Following pupation, the gut microbiota exhibited diversified functions, likely due to the complex environments and diverse food sources encountered after metamorphosis. These functions included amino acid metabolism, compound degradation, and defense mechanisms. This research provides an extensive dataset on the gut microbiota during the metamorphosis of Vespa mandarinia, contributing to a deeper understanding of the influence of gut microbiota on wasp growth. Furthermore, this study uncovers a unique microbial treasure within insect guts, which is important for advancing the application of wasps in the fields of food and medicine. [ABSTRACT FROM AUTHOR]

Simple Summary: We conducted a study on flightin, a vital protein found in insect flight muscles, with a specific focus on the social wasp Vespa basalis, which is a dangerous hornet species. Using sequence analysis techniques, we successfully obtained an 1189 bp cDNA sequence encoding the flightin protein consisting of 150 amino acids. According to our analysis, the molecular weight and isoelectric point of the amino acid sequence are 18.05 kDa and 5.84, respectively. The protein lacks a transmembrane topology structure, and we identified four patterns of functional sites. Conducting phylogenetic analysis on 38 species, including 8 Vespidae species, revealed a close evolutionary relationship between Vespa basalis and Vespa mandarinia. These findings warrant further investigation, especially considering the contrasting information obtained from the analysis of mitochondrial sequences. Flight is a complex physiological process requiring precise coordination of muscular contraction. A key protein in insect flight is flightin, which plays an integral role in the flight muscles. This research sought to evaluate the flight competence of the social wasp V. basalis by characterizing the molecular components involved. Our study focused on Vespa basalis, one of the most dangerous hornet species, utilizing PCR to obtain a partial cDNA sequence of the flightin protein. We then employed phylogenetic and sequence analysis to gain insights into this protein in flight-related adaptations. The cDNA has an 1189-base pair sequence including an open reading frame (453 bp) encoding 150 amino acids. Analyzing the deduced amino acid sequence using an online tool revealed a molecular weight of 18.05 kDa, an isoelectric point of 5.84, four functional site patterns, and no transmembrane topology. We constructed a phylogenetic tree of flightin based on 38 species. Our analysis indicated that V. basalis is most closely related to V. mandarinia; this alignment is consistent with their similar aggressive behavior, but their evolutionary relationship, based on mitochondrial sequences, presents a contrast. These initial findings on the flightin gene in V. basalis lay the groundwork for future functional studies to elucidate its specific role in flight adaptations and explore its potential as a target for pest management strategies. [ABSTRACT FROM AUTHOR]

The study on insect pollinators of litchi, revealed 227 specimens of insect fauna belonging to 24 species of six different orders and 15 families. Hymenoptera, (belonging to Apidae, Andrenidae, Megachilidae, Vespidae and Sphecidae) was the most dominant (72.68%), followed by Diptera (19.38%), Coleoptera (3.08%), Lepidoptera (2.2%), Hemipter (1.76%) and the lowest, Odonata (0.88%). Among Hymenopterans, honeybees were the pre-dominant insect pollinators (72.68%), viz., Apis florea (37%), A. cerana (15.41%), A. mellifera (7.04%) and A. dorsata (3.08%) on litchi bloom. Foraging activity of A. mellifera began early in the morning (mean 5 53h) and cessation of flight took place at evening (mean 18 01h). While the mean foraging speed of A. mellifera was maximum at 9 00h, the minimum foraging speed was at 17 00h. Maximum foraging rate was observed at 17 00 and minimum at 1100h. [ABSTRACT FROM AUTHOR]

The taxonomic validity of Dolichovespula kuami, especially in relation to D. flora, has been the subject of a long-term debate. Herein, the valid specific status of the former was supported through an integrated analysis of morphological characters and DNA barcodes. The pronotal rugae and male genitalia of the two species are different, and partial mitochondrial genes (cytochrome oxidase subunit I, COI) indicate that they form significantly distinct lineages. The hitherto unknown male of D. kuami is described for the first time, and a brief discussion of the D. maculata species group is provided. [ABSTRACT FROM AUTHOR]

A cladistic analysis of the species of the Neotropical social wasp genus Agelaia Lepeletier, 1836, was performed employing female morphology and nest architecture data. Analysis resulted in a single cladogram with length 339, consistency index 0.22, and retention index 0.60, supporting Agelaia as monophyletic. Agelaia bequaerti and A. anceps are raised to specific rank. Male genitalia of A. angulata, A. areata, A. cajennensis, A. centralis, A. flavipennis, A. fulvofasciata, A. multipicta, A. ornata, A. pallipes, A. panamaenis, A. testacea, A. timida, A. vicina, and A. yepocapa are depicted and described, including comparative remarks. A revised identification key is provided. [ABSTRACT FROM AUTHOR]

New data are presented for the potter wasp genus Orancistrocerus van der Vecht (Eumeninae, Odynerini) occurring in Vietnam. Two species are described as new to science: Orancistrocerus thanhnhat sp. nov. and O. thanghen sp. nov. Orancistrocerus aterrimus erythropus van der Vecht is synonymized with Orancistrocerus aterrimus aterrimus (de Saussure); the male genitalia of this species are described for the first time. An updated key is presented to all species of the genus. [ABSTRACT FROM AUTHOR]

As the social organism, Polistes wasps build a communal nest using woody fibers with saliva for sustaining brood and adult population throughout the season. Limited information exists regarding the identification specific plant materials employed in wasp nest building. Thus, we firstly tested if the DNA metabarcoding approach utilizing rbcL and trnL molecular markers could identify the plant species quantitatively and qualitatively inform the mixed-origin woody samples. A threshold of 0.01 proportion of reads was applied for rbcL and trnL molecular markers, while this threshold for median proportion was 0.0025. In assessing taxa richness, the median proportion demonstrated superior performance, exhibiting higher taxa detection power, however, rbcL marker outperformed in quantitative analysis. Subsequently, we applied DNA metabarcoding to identify the plant materials from the nests of two Polistes species, P. mandarinus and P. rothneyi. The results showed that higher preference of Quercus and Robinia as the major nest building materials regardless of the surrounding plant communities, by two wasp species. Material diversity was higher for P. rothneyi than P. mandarinus, which may explain the abundance of this species possibly with heightened adaptive capacities in their nesting behavior. This study demonstrated that DNA metabarcoding could identify the complex nest-building plant materials of paper wasps and provide insights into their ecological interactions in the natural ecosystem. [ABSTRACT FROM AUTHOR]

Social wasps (Family: Vespidae) are opportunistic and generalist feeders, which can occasionally feed on live vertebrates. The German yellowjacket (Vespula germanica) is native to Eurasia and Northern Africa, and has invaded many countries around the world, producing several environmental, economic and social impacts. Here we report the first video‐recorded evidence that this wasp species preys on chicks. Over six breeding seasons, we studied the parental care behaviour of the Chilean Elaenia (Elaenia chilensis), a long‐distance migratory bird that breeds in the Andean‐Patagonian Forest. We filmed 59 nests when chicks were 2–3 and 10–11 days old, and recorded in a nest containing two 10‐day‐old nestlings the predation of one of them by German yellowjackets. The other chick was found alive on the ground, indicating that it escaped from the wasps. Parents did not defend offspring against wasp attacks. The rarity of this observation may suggest either that this insect is not yet a significant nest predator or that it is difficult to obtain a record of predation by wasps, because birds may remove dead nestlings from the nests (i.e. nest sanitation). Although Chilean Elaenia is not threatened, 41% of bird species in the Andean‐Patagonian Forest are endemic, and this new nest predator may potentially become a threat to the conservation of any of those species, which deserves further research. Hence, to evaluate the German yellowjacket impact on bird populations, it is imperative to conduct videotaped nest monitoring in order to remove the masking effect of nest sanitation on predator identity, which will allow to determine how much this wasp contributes to nest failure in the invaded zone of the Neotropical region. [ABSTRACT FROM AUTHOR]

Ropalidia chromis sp. nov. is described from the Democratic Republic of the Congo. It is characterized by a mixture of morphological features present in two large species groups of that genus, suggesting a separate phylogenetic lineage. [ABSTRACT FROM AUTHOR]

Dispersal is a multifactorial process which may influence, among several others, population dynamics, inbreeding likelihood, and mate competition. For species that disperse by flight, many studies have suggested that life‐history traits, such as body size or nutritional condition, could promote variation in flight capabilities and, consequently, on dispersal behavior. Eusocial hymenopterans are likely to experience high densities of related reproductive individuals near their nests, thus increasing probabilities of inbred matings and kin competition. Spatial segregation resulting from differences in flight capabilities of Vespula germanica (Fabricius) (Hymenoptera: Vespidae) nestmate drones could reduce interactions with kin, thus decrease inbreeding and kin competition. Our aim was to characterize, in the laboratory, flight capabilities of sibling drones and elucidate traits that could affect it, focusing on the relevance of carbohydrate intake (diets with different sugar concentration) during early adulthood. Our results indicate that in tethered flight mill assays, V. germanica drones have highly variable flight capabilities within and between nests, with most drones likely to remain relatively close to their nest of origin. The observed flight capabilities are affected by nutrition, with those individuals better nurtured (i.e., with higher nutritional index), flying farther distances. Additionally, diets with variable carbohydrate concentration provided during early adulthood did not affect flight capabilities (only those exposed to a carbohydrate‐free diet had reduced flight distances and speeds), suggesting that nutrition during larval stages is determinant for flight. Our findings contribute to the understanding of the mechanisms underlying V. germanica drone dispersal behavior which, in turn, might influence inbreeding likelihood in the species. [ABSTRACT FROM AUTHOR]

In social insects, post-hibernation queens of independent-founding species (e.g. bumble bees, some ants and wasps) need to overcome various difficulties for survival and reproduction during the initial period of the colony. Specifically, a single queen must perform all tasks related to colony defense and nursing juveniles alone. In this study, we examined whether the reproductive conditions of post-hibernation solitary queens of the vespine wasp Vespa analis are affected by the following four factors: (1) collection date, (2) mating status, (3) body size and (4) the presence of Xenos parasites. The results showed that ovary development of the queens was associated with collection date and mating status but not with body size or parasitism. The positive effects of the first two factors are reasonable, because they are closely related to life history, but the absence of a link between ovary development of the queens and parasitism was surprising. No association of ovary development of the queens with body size prompts the need for more comparative studies, which might reveal differences in the fecundity-body size relationship among temperate Vespa species. [ABSTRACT FROM AUTHOR]

The first recordings in South Africa of the invasive social wasps, Vespula germanica and Polistes dominula (Hymenoptera: Vespidae) were in 1974 and again in 2008. These wasps are known to represent a significant threat to the biodiversity of ecosystems and socio-economic activities in countries they invade. Here, the susceptibility of V. germanica and P. dominula larvae to indigenous species of entomopathogenic nematodes (EPNs), namely Heterorhabditis bacteriophora, H. noenieputensis and Steinernema yirgalemense, and entomopathogenic fungus (EPF) Beauveria bassiana was tested both in laboratory bioassays and in situ wasp nests application in the Western Cape province. The bioassay results indicate that both P. dominula and V. germanica larvae are susceptible to all biocontrol agents used. All larvae of both wasp species were dead and infected after exposure to 200 IJ/insect within four days after inoculation. Similar results were obtained seven days after inoculation with B. bassiana. To test in-field pathogenicity against P. dominula, wasp nests were treated with B. bassiana, H. bacteriophora, a mixture of the EPF and EPN species and a control of distilled water. The number of larvae and pupae infected 168 h after application were determined. In both cases, the mixture of EPF/EPN performed best, with a mean percentage of 31.39% ± 4.75% larvae infected, and only 3.42% ± 0.68% of pupae infected. The results obtained suggest that higher concentrations of selected biologicals can be used as inundative biological control agents within an integrated management programme for the control of P. dominula and V. germanica in South Africa. Highlights At 48 h after inoculation, 100% of the wasp larvae treated with EPN were infected After 168 h, 74–98% of EPF-treated larvae showed visual infection of Beauveria bassiana Heterorhabditis bacteriophora outperformed other biocontrol agents tested Combined EPN and EPF in situ nest application achieved larval infection of 31% ± 4.75%. [ABSTRACT FROM AUTHOR]

Mitochondrial (mtDNA) genes have served as widely utilised genetic loci for phylogenetic and phylogeographic studies of animals. However, the phylogenetic performance of many mtDNA genes has not been empirically evaluated across lineages within hymenopteran wasps. To address this question, we assembled and analysed mitogenomic data from social wasps, representing the four recognised tribes of Polistinae and all Epiponini genera. Additionally, we evaluated whether mtDNA gene order in Polistinae is congruent with its tribal classification. Using concatenation phylogenetic methods, we show phylogenetic congruence between mitogenomic and nuclear data. Statistically comparing the phylogenetic performance of individual mtDNA genes, we demonstrate that for social wasps the molecular markers COI, 16S, NAD5, and NAD2 perform best, while ATP6, COII, and 12S show the worst results. Finally, we verified that the tRNA cluster close to the noncoding region is a hotspot of genetic rearrangements in Vespidae and can be used as additional information for the systematics of this group. Together, these results indicate that mitogenomes contain robust phylogenetic signal to elucidate the evolutionary history of Vespidae. Moreover, our study identifies the best choice of mtDNA markers for systematic investigations of social wasps. [ABSTRACT FROM AUTHOR]

Interações entre aves e vespas sociais têm sido relatadas nas regiões neotropicais, entretanto, a frequência e os fatores promotores dessa interação ainda não são total- mente compreendidos, mas têm sido justificados por um suposto benefício para as aves contra seus predadores fornecido pelas vespas sociais. Existem poucos registros de pássaros da família Furnariidae associando à vespas sociais, diante disso, esse estudo relata a inédita interação entre o pássaro Certhiaxis cinnamomeus e a vespa social Polybia scutellaris em ecótono entre Mata Atlântica e Cerrado, sudeste do Brasil, registrado em 2022. A possível associação entre C. cinnamomeus e vespas sociais, aparentemente não ocorre de forma ocasional e isolada, envolvendo provavelmente outras espécies de vespas sociais, por isso sugere-se que seja melhor investigada para melhor compreensão dos fatores que a promovem.

The European paper wasp, Polistes dominula (Christ) (Hymenoptera: Vespidae), directly competes with native Polistes and is a safety concern for workers and homeowners. A previous investigation of Polistes behavior found that a portion of wasps died after consuming a solution of pear ester, a compound commonly found in fruits that is considered non-toxic to humans. The goal of our study was to corroborate those preliminary observations and investigate the toxicity of pear ester to P. dominula. Two experiments were performed. First, 3 wasp castes were fed 17% sugar water, or sugar water mixed with 0.5%, 1.0%, 5.0%, or 10.0% (w/v) solutions of pear ester. Mortality after 24 h ranged from 0% among wasps fed sugar water only, to >10% among wasps that were fed pear ester. Mortality increased logarithmically with pear ester concentration. Pear ester toxicity differed among wasp castes, with <5% mortality recorded among fall gynes to >10% mortality among workers. The second experiment attempted to define the relationship between morbidity and volume of pear ester consumed by P. dominula workers. Although results confirmed that ingestion of pear ester causes death or paralysis of P. dominula workers, there was no clear relationship between volume of ingested pear ester and wasp morbidity. About 80% of P. dominula were unaffected by pear ester despite consuming large quantities of the solution. This is the first study to demonstrate lethal effects of pear ester compound and may indicate its potential as an insecticide for paper wasps. La avispa cartonera europea, Polistes dominula (Christ) (Hymenoptera: Vespidae), compite directamente con las Polistes nativas y, además, es una preocupación de seguridad para trabajadores y familias en sus casas. Una investigación previa sobre el comportamiento de Polistes, encontró que algunas de las avispas morían después de consumir una solución de éster de pera, un compuesto comúnmente encontrado en frutas y que no es considerado tóxica para humanos. El objetivo de nuestro estudio fue corroborar esas observaciones preliminares e investigar la toxicidad del éster de pera para P. dominula. Se realizaron 2 experimentos. Primero, 3 castas de avispas fueron alimentadas con soluciones de 17% agua azucarada, o agua mezclada con 0.5%, 1.0%, 5.0% y 10.0% (p/v) con éster de pera. La mortalidad después de 24 horas varió desde 0% entre avispas alimentadas solamente con agua azucarada, a >10% entre avispas a las cuáles se les dio éster de pera. La mortalidad aumentó logarítmicamente con la concentración de éster de pera. La toxicidad del éster de pera varió entre las castas de avispas, con una mortalidad registrada <5% entre hembras reproductivas de otoño a >10% entre obreras. El segundo experimento intentó definir la relación entre morbilidad y volumen de éster de pera consumido por obreras de P. dominula. Aunque los resultados confirmaron que la ingesta de éster de pera causa la muerte o parálisis de obreras de P. dominula, no hubo una relación clara entre el volumen ingerido de éster de pera y morbilidad. Cerca del 80% de P. dominula no fue afectado por el éster de pera, a pesar, de haber consumido grandes cantidades de la solución. Este es el primer estudio que demuestra que la ingestión de éster de, pero por un insecto causa parálisis o muerte. Este es el primer estudio que demuestra efectos letales del compuesto de éster de pera y puede indicar su potencial como insecticidas para avispas papeleras. [ABSTRACT FROM AUTHOR]

Polistes dominula is an invasive social wasp that preys on the grasshopper Dichroplus elongatus, which constitutes a potential agricultural pest in Northwest Patagonia. This grasshopper feeds on a wide variety of plants, the 'Dandelion' (Taraxacum officinale) being one of the main food resources. In this study, by means of a Y‐tube olfactometer, we analysed the use of olfactory cues by P. dominula in the detection of grasshoppers. Specifically, we evaluated the attractiveness of live grasshoppers, their traces (i.e. leaves with herbivory traces + prey faeces or only faeces) and herbivory‐free leaves. The results showed that foragers equally chose olfactory cues emitted by grasshoppers and leaves with herbivory traces + faeces and herbivory‐free leaves, suggesting that the leaves of the host plant also have an important role in the searching behaviour. The current research is the first to demonstrate that the invasive paper wasp orients its search towards either the presence of grasshopper prey or the volatiles of plant leaves. These findings shed new light on predator‐prey interactions mediated by olfactory cues in P. dominula, which may play a biological role as a natural controller of D. elongatus, phytophagous pest in the invaded ecosystems. [ABSTRACT FROM AUTHOR]