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Your search keyword '"VALLARI, Ana S."' showing total 13 results
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13 results on '"VALLARI, Ana S."'

1. Characterization of HBV surface antigen isoforms in the natural history and treatment of HBV infection

Author

Rodgers, Mary A., primary, Shah, Pir A., additional, Anderson, Mark, additional, Vallari, Ana S., additional, Gersch, Jeffrey, additional, Mbanya, Dora, additional, Sauleda Oliveras, Silvia, additional, Choudhry, Saad, additional, Leary, Thomas P., additional, Kuhns, Mary C., additional, Dawson, George J., additional, Cloherty, Gavin A., additional, and Lau, Daryl T.Y., additional

Published
2023
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5. ARCHITECT HIV Combo Ag/Ab and RealTime HIV-1 Assays Detect Diverse HIV Strains in Clinical Specimens

Author

Rodgers, Mary A., primary, Vallari, Ana S., additional, Yamaguchi, Julie, additional, Holzmayer, Vera, additional, Harris, Barbara, additional, Toure-Kane, Coumba, additional, Mboup, Souleymane, additional, Badreddine, Samar, additional, McArthur, Carole, additional, Ndembi, Nicaise, additional, Mbanya, Dora, additional, Kaptue, Lazare, additional, and Cloherty, Gavin, additional

Published
2018
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6. Use of a Multifaceted Approach to Analyze HIV Incidence in a Cohort Study of Women in the United States: HIV Prevention Trials Network 064 Study

Author

Eshleman, Susan H., primary, Hughes, James P., additional, Laeyendecker, Oliver, additional, Wang, Jing, additional, Brookmeyer, Ron, additional, Johnson-Lewis, LeTanya, additional, Mullis, Caroline E., additional, Hackett, John, additional, Vallari, Ana S., additional, Justman, Jessica, additional, and Hodder, Sally, additional

Published
2012
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13. MOLECULAR ANALYSES OF HIV-1 GROUP O AND HIV-2 VARIANTS FROM AFRICA.

Author

HUNT, Jeffrey C., BRENNAN, Catherine A., GOLDEN, Alan M., YAMAGUCHI, Julie, LUND, Jennifer K., VALLARI, Ana S., HICKMAN, Robert K., ZEKENG, Leopold, GÜRTLER, Lutz G., HAMPL, Hartmut, KAPTUÉ, Lazare, and DEVARE, Sushil G.

Subjects
HIV, NUCLEOTIDE sequence, SEROLOGY, RNA, VIRAL envelopes
Abstract

Genetic variation among HIV isolates creates challenges for their detection by serologic and genetic techniques. To characterize the sequence variation and its correlation to serologic diversity of HIV-1 Group O and HIV-2 isolates, samples were identified by differential reactivity in selected commercial and research assays. Analysis of sera from Equatorial Guinea (EG) led to identification of 4 HIV-1 Group O variants. Viral RNA, extracted from these samples was used to PCR amplify overlapping sequences of the entire envelope gene using multiple primer pairs. Sequence analysis indicated that the V3 loop nucleotide and protein sequences aligned more closely with HIVANT70 compared to other Group O sequences. The amino acid sequences at the octameric tip of the V3 loop were RIGPLAWY, RIGPMAWY, or GLGPLAVY. The tetrameric tip GPLA is represented only once in the published 1994 HIV database (Los Alamos) but was present in 2 of 4 of EG samples. The immuno-dominant region (IDR) sequences derived from EG sera were unique in that none of the sequences were completely homologous to other HIV-1 group O variants. Further, the HIV-1 group O sequence variation could be correlated with differential serologic reactivity using IDR peptides. Compared to HIV-1, the sequence information on HIV-2 isolates is relatively limited, though the HIV-2 isolates also show genetic variation similar to HIV-I. To further establish a correlation between the genetic diversity and serologic detection of HIV-2, plasma samples from Western Africa were evaluated. Eight samples were selected based on weak serologic reactivity to env proteins. PCR amplification and sequence analysis of the gag;, env V3 loop, and env IDR regions indicated that the samples could be classified as subtypes A (4 samples), B (3 samples) and D (1 sample). Across the subtypes, there was conservation in the IDR region of the sequence WGCAFRQVCHT. This region is absolutely conserved among the majority of currently known HIV-2 and related SIV viruses (1994 HIV database). One subtype B sample had a unique sequence immediately adjacent to the IDR, however, this did not change the serologic detection using a HIV-2 IDR specific monoclonal antibody. [ABSTRACT FROM AUTHOR]

Published
1997

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