1. Prevalence of Serological and Molecular-Biological HIV-Infection, HBV and HCV Markers among Medical Workers
- Author
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E. V. Anufrieva, Yu. V. Ostankova, E. N. Serikova, A. N. Shchemelev, V. S. Davydenko, D. E. Reingardt, E. B. Zueva, and Areg A. Totolian
- Subjects
viral hepatitis ,hcv ,hbv ,hiv infection ,serological markers ,molecular-biological markers ,health care workers ,laboratory diagnosis ,Infectious and parasitic diseases ,RC109-216 - Abstract
The aim of our work was to assess the occurrence of serological and molecular-biological markers of HIV, HBV and HCV among medical workers from St. Petersburg. Materials and methods. The study material included 171 blood plasma samples from medical workers from St. Petersburg. The ELISA method was used to qualitatively determine HIV antigen/antibodies (Ag/Ab), HBsAg, antibodies to HBs and HBcore proteins (anti-HBs IgG, anti-HBcore total), and anti-HCV IgG. HIV RNA, HCV RNA and HBV DNA were detected by PCR. For all identified HBV samples, sequencing and subsequent analysis of the nucleotide sequences of the Pre-S1/Pre-S2/S region were performed. Results and discussion. The surveyed group was ranked by age into the following subgroups: 21–35 years (28.07 %), 36–50 years (26.90 %), 51–65 years (31 %) and over 66 years (13.45 %). Serological markers of HBV were detected in 91 (53.22 %) subjects, HCV – in 2 (1.17 %). No markers of HIV were detected. At the same time, markers for HBV and HCV were found in 1 (0.58 %) person. HBV DNA was detected in 4 (2.34 %) medical workers; HCV RNA and HIV were not detected. Significant differences in the prevalence and distribution of the analyzed serological markers between age groups were determined. All HBV isolates belong to genotype D, subgenotypes D1 (50 %) and D2 (50 %). High variability of the Pre-S1/Pre-S2/S region of HBV in the examined group has been shown. The results obtained indicate a relatively low prevalence of HBV and HCV in the examined group. Monitoring of the prevalence of blood-borne infections among medical workers remains an urgent task, primarily in relation to HBV.
- Published
- 2024
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