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Aim. Acceleration of the sunflower lines homozygosity for isolation of the fertility restorer resistant to tribenuron methyl herbicide using in vitro culture of sunflower immature embryos. Methods. Methods of immature sunflower embryos cultivation in in vitro culture; methods of classical crossing. Results. As a result, homozygous genotype was created and isolated from three combinations (BH0118xSURES-2, BH0218xSURES-2 and BH0318xSURES-2) for 10 parent sunflower lines. Conclusions. The use of the technology of sunflower immature embryos cultivation in in vitro culture effectively accelerates the sunflower lines homozygosity for the fertility restorer isolation resistant to tribenuron methyl herbicide. The involvement of immature sunflower embryos cultivation in in vitro culture methods can reduce time for creation of initial parental sunflower lines twice. Keywords: sunflower, immature embryos, in vitro culture, herbicide resistance, tribenuron methyl.

The article presents results of environmental trials of «VNIS», imidazolinone (IMI) and tribenuron - methyl (SU) - resistant sunflower hybrids, which were conducted in the Forest-Steppe and Southern Steppe of Ukraine. In regions with contrasting climatic conditions, the variability parameters of basic agronomic traits (yield, oil content of seeds, oil production per hectare) were determined in hybrids. The growing conditions comfort was assessed by variability of the traits at each test site. The specificity of the hybrid response (IMI, SU) in the formation of traits under contrast agroclimatic conditions of cultivation was revealed. For the imidazolinone - resistant hybrids, the conditions of the Kyiv region (Forest - Steppe) were the most comfortable, judging from their yields and oil product per hectare. The conditions of the Kherson region (Southern Steppe) were the best for oil content of seeds. For the tribenuron - methyl-resistant hybrids, the Southern Steppe (Mykolaiv region) was the most suitable to produce high yields and oil product per hectare, and the Forest Steppe (Kyiv region) – to accumulate high contents of oil in seeds.Comparisons of hybrids of different chemical resistance groups revealed that the IMI - resistant hybrids outperformed the SU - resistant ones in terms of yield and oil production per hectare at each test site.Comparative characteristics of expression levels (high, medium, low) of each trait of the hybrids were defined for the herbicide resistance groups under investigation. The difference in the yield between the imidazolinone - resistant hybrids and the tribenuron - methyl-resistant hybrids was 0,3–0,44 t/ha, depending on the expression group. As to oil content of seeds, the SU - resistant hybrids were also inferior to the IMI - resistant ones, ranging from 0,5 % in the medium expression group to 0?18% in the low expression group. As to oil product, the IMI - resistant hybrids outperformed the SU - hybrids by 968,0–1583,0 kg/ha, depending on the expression level the trait. The environment indices demonstrated that in the arid conditions of the Southern Steppe the imidazolinone - resistant hybrids accumulated more oil in seeds. The hybrids’ types of environmental plasticity and stability were determined for the basic breeding-significant for production traits.Promising for production sunflower hybrids of various plasticity and regional orientation, which are resistant to imidazolinones and tribenuron - methyl and combine high yields, oil contents of seeds and oil product per hectare, were distinguished.

Purpose.Determine the ecological plasticity and productivity of F1sunflower hybrids created on the basis of maternal and parental lines, selected according to an accelerated selection system of lines resistant to herbicides (imidazoline and sulfonylurea groups) and broomrape (Orobanche cumanaWallr.). Methods.Statistical analysis of F1sunflower hybrids was carried out using the methods of variation statistics, regression and analysis of variance using the Microsoft Office Excel 2016 application package. Molecular biological, biotechnological and classical selection methods were used for the accelerated system of line selection. Thus, for the purpose of targeted selection of sunflower sterility fixers, we used HRG01 molecular SCAR marker to identify the gene for the restoration of pollen fertility (Rf1). To accelerate the creation of parental lines resistant to tribenuron-methyl, we used a culture of immature embryosin vitro. Results.The results of testing of F1sunflower hybrids at Kyiv, Chernihiv, Cherkasy (Uman and Shpolianskyi districts), Khmelnytskyi, Kharkiv, Kherson and Odesa regions. The hybrids were created on the basis of selected lines, chosen according to an accelerated selection system for herbicide-resistant lines (imidazoline (IMI-hybrids) and sulfonylurea (SU-hybrids) groups) and broomrape (Orobanche cumanaWall). The standards for comparison with hybrids were: for IMI hybrids – hybrids ‘NK Neoma’ (Syngenta) and‘ES Genesis’ (Euralis), and for SU-hybrids – ‘SY Sumiko’ (Syngenta) and ‘P64LE25’ (Pioneer). As a result, it was found that among SU-hybrids, UA 2/106 had a 3.9% higher yield when compared to the standards (‘SY Sumiko’ and ‘P64LE25’). And for IMI-hybrids it was found that hybrids UA 1/67, UA 1/66, UA 1/84 have the same yield of 2.76 t/ha as the ‘NK Neoma’ standard. IMI hybrids UA 1/92, UA 1/102 have the same yield of 2.91 t/ha as ‘ES Genesis’. Conclusions.F1hybrids were created on the basis of the original breeding material selected due to the accelerated system of sunflower lines selection. The hybrids were analyzed according to the yield indicator. The most productive among the tested SU-hybrids was UA 2/106 hybrid, among the IMI hybrids – UA 1/67, UA 1/66, UA 1/84, UA 1/92 and UA 1/102.