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Chemoprevention has emerged as a very effective preventive measure against carcinogenesis. Many bioactive compounds present in edible as well in herbal plants have revealed their cancer chemopreventive potential. In the present study, our goal was to investigate the impact of piperine, a principle ingredient of pepper, on alterations of mitochondrial antioxidant system and lipid peroxidation in Benzo(a)pyrene (B(a)P) induced experimental lung carcinogenesis. Oral supplementation of piperine (50 mg/kg body weight) effectively suppressed lung carcinogenesis in B(a)p induced mice as revealed by the decrease in the extent of mitochondrial lipid peroxidation and concomitant increase in the activities of enzymatic antioxidants (superoxide dismutase, catalase and glutathione peroxidase) and non enzymatic antioxidant (reduced glutathione, vitamin E and vitamin C) levels when compared to lung carcinogenesis bearing animals. Our data suggests that piperine may extent its chemopreventive effect by modulating lipid peroxidation and augmenting antioxidant defense system. [ABSTRACT FROM AUTHOR]

Animal agriculture is a leading contributor to greenhouse gas emissions and other harmful environmental impacts, which underscores the need to shift away from the consumption of animal-based products. One promising nudge intervention is making plant-based meals the default option, so we tested this approach at six different university events across four academic institutions for effecting sustainable dietary change. Event attendees pre-selected their meal on one of two randomly assigned RSVP forms: one with a plant-based default and one with a meal with meat default. The results from our randomized controlled trial showed that participants had a 43-percentage point greater probability of selecting the plant-based meal when it was indicated as the default option. This effect was similar across events and academic institutions, which indicates that this default intervention is generalizable and can be successfully implemented at university events. The combined effect of using plant-based defaults at these six events was an estimated reduction of 104,387 kg of CO2 emissions, 299.9 m 2 of land use, 959.0 g of nitrogen use, and 259.5 g of phosphorus use, which represent roughly 45-46.2% reductions in harmful environmental impacts relative to the meals chosen when using a meat default. Given the significance and magnitude of these environmental benefits, our results support the widespread implementation of plant-based defaults for helping universities improve their sustainability., Competing Interests: Declaration of competing interest No conflict of interest., (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)

Miniscrews are widely used in orthodontics as an anchorage device while aligning teeth. Shear stress in the miniscrew-bone interface is an important factor when the miniscrew makes contact with the bone. The objective of this study was to analyze the shear stress and force in the screw-bone interface for varying Cortical Bone Thickness (CBT) using Finite Element Analysis (FEA). Varying CBT of 1.09 mm (1.09 CBT ) and 2.66 mm (2.66 CBT ) with miniscrews of Ø1.2 mm, 10 mm length (T1), Ø1.2 mm, 6 mm length (T2) and Ø1.6 mm, 8 mm length (T3) were analyzed. Six Finite Element (FE) models were developed with cortical, cancellous bone, miniscrews and gingiva as a prism. A deflection of 0.1 mm was applied on the neck of the miniscrews at 0°, +30° and -30° angles. The shear stress and force in the screw-bone interface were assessed. The results showed that the CBT affects the shear stress and force in the screw-bone interface region in addition to the screw dimensions and deflection angulations. T1 screw generated lesser shear stress in 1.09 CBT and 2.66 CBT compared to T2 and T3 screws. Higher CBT is preferred for better primary stability in shear aspect. Clinically applied forces of 200 gms to 300 gms to an anchorage device induces shear stress in the miniscrew-bone interface region might cause stress shielding. Thus, clinicians need to consider the effect of varying CBT and the size of the miniscrews for the stability, reduced stress shielding and better anchorage during orthodontic treatment., (© 2024 IOP Publishing Ltd.)

Alcohol is a dangerous substance causing global mortality and health issues, including mental health problems. Regular alcohol consumption can lead to depression, anxiety, cognitive decline, and increased risk of alcohol-related disorders. Thus, monitoring ethanol levels in biological samples could contribute to maintaining good health. Herein, we developed an electrochemical sensor for the determination of ethanol in human salivary samples. Initially, the tetra-chloroauric acid (HAuCl 4 ) was chemically reduced using sparfloxacin (Sp) which also served as a stabilizing agent for the gold nanoparticles (AuNPs). As-prepared Sp-AuNPs were comprehensively characterized and confirmed by UV-visible spectroscopy, X-ray diffraction, field emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDS), and elemental mapping analysis. The average particle size (~25 nm) and surface charge (negative) of Sp-AuNPs were determined by using dynamic light scattering (DLS) and Zeta potential measurements. An activated screen-printed carbon electrode (A-SPE) was modified using Sp-AuNPs dispersion, which exhibited greater electrocatalytic activity and sensitivity for ethanol (EtOH) oxidation in 0.1 M sodium hydroxide (NaOH) as studied by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). DPV showed a linear response for EtOH from 25 µM to 350 µM with the lowest limit of detection (LOD) of 0.55 µM. Reproducibility and repeatability studies revealed that the Sp-AuNPs/A-SPEs were highly stable and very sensitive to EtOH detection. Additionally, the successful electrochemical determination of EtOH in a saliva sample was carried out. The recovery rate of EtOH spiked in the saliva sample was found to be 99.6%. Thus, the incorporation of Sp-AuNPs within sensors could provide new possibilities in the development of ethanol sensors with an improved level of precision and accuracy.

Nicotine is the one of the major addictive substances; the overdose of nicotine (NIC) consumption causes increasing heart rate, blood pressure, stroke, lung cancer, and respiratory illnesses. In this study, we have developed a precise and sensitive electrochemical sensor for nicotine detection in saliva samples. It was built on a glassy carbon electrode (GCE) modified with graphene (Gr), iron (III) phthalocyanine-4,4',4″,4'''-tetrasulfonic acid (Fe(III)Pc), and gold nanoparticles (AuNPs/Fe(III)Pc/Gr/GCE). The AuNPs/Fe(III)Pc/Gr nanocomposite was prepared and characterized by using FE-SEM, EDX, and E-mapping techniques to confirm the composite formation as well as the even distribution of elements. Furthermore, the newly prepared AuNPs/Fe(III)Pc/Gr/GCE-nanocomposite-based sensor was used to detect the nicotine in phosphate-buffered solution (0.1 M PBS, pH 7.4). The AuNPs/Fe(III)Pc/Gr/GCE-based sensor offered a linear response against NIC from 0.5 to 27 µM with a limit of detection (LOD) of 17 nM using the amperometry (i-t curve) technique. This electrochemical sensor demonstrated astounding selectivity and sensitivity during NIC detection in the presence of common interfering molecules in 0.1 M PBS. Moreover, the effect of pH on NIC electro-oxidation was studied, which indicated that PBS with pH 7.4 was the best medium for NIC determination. Finally, the AuNPs/Fe(III)Pc/Gr/GCE sensor was used to accurately determine NIC concentration in human saliva samples, and the recovery percentages were also calculated.

Background and Aim: Biting forces from the teeth are distributed to the facial bones and to the skull through the stress trajectories. The presence of a bony defect in either the maxilla or mandible might lead to variations in the stress distribution. The aim of this study was to evaluate the stress distribution and variations in stress trajectories from biting forces in a human skull with maxillofacial defect using a finite element (FE) model., Methodology: In this study, a cone beam computed tomography (CBCT) of an adult male patient with a maxillectomy defect consequent to surgical treatment of mucormycosis is evaluated for the stress distribution from the biting forces of the remaining posterior teeth. Finite element model without the mandible was constructed from the patient's CBCT data. Occlusal loading forces of 600 N were applied on each side of the maxillary teeth. Stress trajectories were visualised through the stress distribution pattern., Results: The results showed deviations in the normal stress distribution during occlusal loading and variations in zygomatic and pterygoid stress trajectories in the maxillofacial and skull regions in our FE model due to the bony defect., Conclusion: We conclude that a skeletal maxillofacial defect should be reconstructed to resume proper stress distribution during functional forces to maintain a healthy craniofacial skeleton., Competing Interests: Conflict of interestNone of the authors have any financial or personal interest associated with this article. No funding has been received by any author for this study., (© The Association of Oral and Maxillofacial Surgeons of India 2023, Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.)

High concentrations of nicotine (40 to 60 mg) are more dangerous for adults who weigh about 70 kg. Herein, we developed an electrochemical transducer using an MXene (Ti 3 C 2 Tx)/palladium hydroxide-supported carbon (Pearlman's catalyst) composite (MXene/Pd(OH) 2 /C) for the identification of nicotine levels in human sweat. Firstly, the MXene was doped with Pd(OH) 2 /C (PHC) by mechanical grinding followed by an ultrasonication process to obtain the MXene/PHC composite. Secondly, XRD, Raman, FE-SEM, EDS and E-mapping analysis were utilized to confirm the successful formation of MXene/PHC composite. Using MXene/PHC composite dispersion, an MXene/PHC composite-modified glassy carbon electrode (MXene/PHC/GCE) was prepared, which showed high sensitivity as well as selectivity towards nicotine (300 µM NIC) oxidation in 0.1 M phosphate buffer (pH = 7.4) by cyclic voltammetry (CV) and amperometry. The MXene/PHC/GCE had reduced the over potential of nicotine oxidation (about 200 mV) and also enhanced the oxidation peak current (8.9 µA) compared to bare/GCE (2.1 µA) and MXene/GCE (5.5 µA). Moreover, the optimized experimental condition was used for the quantification of NIC from 0.25 µM to 37.5 µM. The limit of detection (LOD) and sensitivity were 27 nM and 0.286 µA µM -1 cm 2 , respectively. The MXene/PHC/GCE was also tested in the presence of Na + , Mg 2+ , Ca 2+ , hydrogen peroxide, acetic acid, ascorbic acid, dopamine and glucose. These molecules were not interfered during NIC analysis, which indicated the good selectivity of the MXene/PHC/GCE sensor. In addition, electrochemical determination of NIC was successfully carried out in the human sweat samples collected from a tobacco smoker. The recovery percentage of NIC in the sweat sample was 97%. Finally, we concluded that the MXene/PHC composite-based sensor can be prepared for the accurate determination of NIC with high sensitivity, selectivity and stability in human sweat samples.

In orthodontics, the torque generated forces from the rectangular archwires refine the teeth position. Literature shows only linear deformation in brackets during torqueing. The objective of this study was to evaluate a new phenomenon of tie wings rotation, an angular deformation in Stainless Steel (SS) brackets with SS and Beta-Titanium (β-Ti) archwires at various angles of twist. Maxillary central incisor SS 0.457 mm × 0.635 mm and 0.558 mm × 0.711 mm brackets, SS and β-Ti archwires of 0.431 mm × 0.635 mm and 0.533 mm × 0.635 mm sizes were used. Finite element analysis was performed in various bracket-archwire assemblies for simulated torque. Palatal root torque was applied and the gingival tie wings rotation was measured at selected points, from 5° to 30° angles of twist. The tie wings rotation for 30° twist with SS 0.533 mm × 0.635 mm archwire in 0.558 mm bracket ranged from 1.32° to 2.55° and with SS 0.431 mm × 0.635 mm archwire in 0.457 mm bracket from 0.71° to 1.73°. Similarly, with β-Ti 0.533 mm × 0.635 mm archwire in 0.558 mm bracket and β-Ti 0.431 mm × 0.635 mm archwire in 0.457 mm bracket, the tie wings rotation ranged from 0.73° to 1.38° and 0.39° to 0.93° respectively. The tie wings rotation were present in all the FE models. Higher archwire size, stiffness, and angles of twist showed increased rotation. Thus, clinicians should be aware of this tie wings rotation during torqueing as an additional factor for torque loss.

Introduction: Torque in orthodontics is the activation of the archwire for the third-order movement of teeth. During this force transfer mechanism from the twisted archwire, the bracket is prone to deformation. This study aimed to compare the deformation in tie wings and the slot region of the bracket during torque using finite element analysis., Methods: Three-dimensionally modeled 0.017 × 0.025-in and 0.019 × 0.025-in stainless steel (SS) and titanium molybdenum alloy archwires were assembled in 0.018-in and 0.022-in solid modeled SS edgewise brackets, respectively. The finite element model of the bracket-archwire combinations was developed with contact boundary conditions. The deformation between tie wings and the slot was analyzed for various angles of twist., Results: For SS archwires at 30° angle of twist, the tie wings deformation in 0.018-in and 0.022-in brackets were 48.67 μm and 34.87 μm, respectively. The slot deformations of 0.018-in and 0.022-in brackets were 66.33 μm and 45.69 μm, respectively. Similarly, the amount of deformation in the bracket-titanium molybdenum alloy archwire combinations were also presented., Conclusions: The slot deformation was more than the tie wings deformation as the slot walls bear the immediate torque force. Thus, orthodontic researchers should know that the torque-relevant bracket deformation should ideally be evaluated in the slot region rather than the tie wings., (Copyright © 2021 American Association of Orthodontists. Published by Elsevier Inc. All rights reserved.)

In orthodontic fixed appliance therapy, the archwire torque used to refine the teeth position during the treatment imparts significant forces inside the bracket slot. The objective of this study was to measure the torque relevant bracket slot deformation in Stainless Steel (SS) brackets during various degree of archwire twist. Standard edgewise brackets 0.018-inch (in.)/0.022-in. each 20 no. and 0.016 × 0.022, 0.017 × 0.025, 0.019 × 0.025, and 0.021 × 0.025 in. archwires each 10 no. were used. A novel experimental setup consisting of loading fixture and torque key mounted on a Vision Measuring System (VMS) were used to measure the brackets slot deformation. The Top Slot and Middle Slot Deformations (TSD and MSD) of the brackets for 35° angle of twist in 0.016 × 0.022 in. archwire in 0.018-in. slot, 0.019 × 0.025 in. archwire in 0.022-in. slot and for 30° angle of twist in 0.017 × 0.025 in. archwire in 0.018-in. slot and 0.021 × 0.025 in. archwire in 0.022-in. slot were measured. Results showed that the mean TSD and MSD were higher when the archwire size, the slot size and the angle of twist were greater. In the evaluated bracket-archwire combinations, the TSD were higher than MSD and the bracket slots were elastically deformed within the clinically required 35° angle of twist in the archwire. Clinicians should be aware of this torque relevant bracket slot deformation which might be a factor for torque loss and suitably incorporate archwire angle of twist.

Background and Objectives: Four tie wings brackets are widely used in orthodontics, while the Six Tie Wings Brackets (STWB) are recently emerging in fixed orthodontic appliances due to their claim for less friction and thus faster teeth movement. The aim of this work was to evaluate the stress distribution and deformation during simulated mesio-distal tipping forces in Stainless Steel (SS) six tie wings orthodontic bracket using Finite Element Analysis (FEA)., Methods: A six tie wings bracket (Synergy®, RMO, USA) dimensions were measured using the Vision system and a 3D model of the bracket was constructed. A Finite Element (FE) model was developed and mesio-distal tipping forces of 1.22 N to 1.96 N (125 to 200 gm) in increments were applied on the gingival and incisal slot walls. The stress distribution and deformation were recorded at specific points in the bracket and analyzed., Results: The maximum deformation and stress distribution for the mesial and distal tipping forces of 1.96 N were recorded as 0.137 µm and 10.60 MPa respectively. The stress concentration was more at the junction of the slot wall and the slot base. For mesial tipping,the deformation was more on the disto-incisal and mesio-gingival tie wings. Similarly, for distal tipping the deformation was more on the mesio-incisal and disto-gingival tie wings. The mid-tie wings showed minimal deformation during both distal and mesial tipping., Conclusions: Our study visualized both the mesial and distal tipping forces induced stress distribution in the bracket tie wing-slot junctions. The deformation was present maximum in the mesio-incisal and disto-incisal tie wings and minimal in the mid-tie wings. Clinicians should be aware of this behavior of STWB in making decisions to alter the tipping forces in the archwire to compensate for the tie wing deformation in refining the teeth position., Competing Interests: Declaration of Competing Interest The authors declare that there is no Conflict of Interest for the manuscript titled “Stress distribution and deformation in six tie wings Orthodontic bracket during simulated tipping – A finite element analysis”., (Copyright © 2020. Published by Elsevier B.V.)

Background and Objectives: Orthodontic fixed appliance therapy involves alignment of teeth through the bracket and archwires. The archwire twist (torque) imparts significant forces inside the bracket slot in refining the teeth position at the end of treatment. The objective of this in- silico study was to evaluate the torque induced bracket slot deformation in the commonly used 0.018 inch (") and 0.022" conventional Stainless Steel (SS) brackets with clinically relevant archwires during various angles of twist., Methods: SS maxillary central incisor brackets of 0.018" width × 0.022" depth (0.457 mm × 0.558 mm) and 0.022" width × 0.028" depth (0.558 mm × 0.711 mm) were used. The SS archwires of 0.016" width × 0.022" depth (0.406 mm × 0.558 mm), 0.017" width × 0.025" depth (0.431 mm × 0.635 mm), 0.019" width × 0.025" depth (0.482 mm × 0.635 mm) and 0.021" width × 0.025" depth (0.533 mm × 0.635 mm) were engaged in the respective bracket slots. The assembled bracket-archwire Finite Element (FE) models were constructed. The archwire torque, the top, middle and bottom slot deformations (TSD, MSD, BSD) were obtained for the bracket-archwire combinations for various angles of archwire twist using FE Analysis (FEA)., Results: The torque, TSD, MSD and BSD for 30 o twist of 0.016" × 0.022" archwire in 0.018" slot were 28.13 Nmm, 35.71 µm, 21.51 µm and 15.67 µm respectively, and for 0.017" × 0.025" archwire were 50.18 Nmm, 54.52 µm, 32.47 µm and 19.11 µm respectively. Similarly for 0.019" × 0.025" archwire in 0.022" slot and 0.021" × 0.025" archwire in 0.022" slot they were 38.82 Nmm, 50.78 µm, 31.47 µm and 16.82 µm, and 60.22 Nmm, 65.22 µm, 36.44 µm and 22.68 µm respectively., Conclusions: The slot deformation was present in both 0.018" and 0.022" brackets which increased as the angle of twist increased. The TSD were higher than the MSD and BSD in all the bracket-archwire combinations. We conclude that there is only elastic deformation of bracket slots upto 30 o angle of twist and clinicians could maintain within this torque limits to avoid plastic deformation leading to improper teeth position., Competing Interests: Declaration of Competing Interest The authors declare that there is no Conflicts of Interest., (Copyright © 2020. Published by Elsevier B.V.)

Introduction: Torque applied on anterior teeth is vital for root positioning and stability. The aim of this study was to evaluate the detailed slot wall deformation in stainless steel (SS) and titanium (Ti) edgewise brackets during palatal root torque using finite element analysis., Methods: A finite element model was developed from a maxillary central incisor SS bracket (0.022 in). The generated torque values from an SS rectangular archwire (0.019 × 0.025 in) while twisting from 5° to 40° were obtained experimentally by a spine tester, and the calculated torque force was applied in the bracket slot. The deformations of the slot walls in both SS and Ti brackets were measured at various locations., Results: There were gradual increases in the deformations of both bracket slot walls from the bottom to top locations. In the SS bracket slot for the 40° twist, the deformations were 9.28, 36.8, and 44.8 μm in the bottom, middle, and top slot wall locations, respectively. Similarly, in the Ti bracket slot for the 40° twist, the deformations were 39.2, 62.4, and 76.2 μm in the bottom, middle, and top slot wall locations, respectively. The elastic limits were reached at 28° for SS and at 37° for Ti., Conclusions: Both SS and Ti bracket slots underwent deformation during torque application. There are variations in the deformations at different locations in the slot walls and between the materials., (Copyright © 2017 American Association of Orthodontists. Published by Elsevier Inc. All rights reserved.)

Ubiquitin C-terminal hydrolase-L1 (UCH-L1), which catalyzes the hydrolysis of ubiquitin esters and amides, is highly expressed in brain. Recently, UCH-L1 has been found to increase cancer cell migration and invasion by modulating hydrogen peroxide generated by NADPH oxidase 4 (NOX4). Because angiogenesis is also mediated by hydrogen peroxide, we explored the role of UCH-L1 in angiogenesis in human umbilical vein endothelial cells (HUVECs). Silencing UCH-L1 suppressed tubule formation in HUVECs, indicating that UCH-L1 promotes angiogenesis in vitro. This was confirmed using in vivo Matrigel plug studies of HUVECs, after overexpressing or silencing UCH-L1. Silencing UCH-L1 significantly suppressed VEGF-induced ROS levels as well as activation of VEGFR, both of which are required for angiogenesis. This study also showed that UCH-L1 promotes angiogenesis of HUVECs, as well as invasion in cancer cells, by up-regulating ROS by deubiquitination of NOX4, suggesting that UCH-L1 plays a key role in angiogenesis of HUVECS by regulating ROS levels by deubiquitination of NOX4., (Copyright © 2017 Elsevier Inc. All rights reserved.)

This study explored the role of ubiquitin C-terminal hydrolase-L1 (UCH-L1) in the production of ROS and tumor invasion. UCH-L1 was found to increase cellular ROS levels and promote cell invasion. Silencing UCH-L1, as well as inhibition of H2O2 generation by catalase or by DPI, a NOX inhibitor, suppressed the migration potential of B16F10 cells, indicating that UCH-L1 promotes cell migration by up-regulating H2O2 generation. Silencing NOX4, which generates H2O2, with siRNA eliminated the effect of UCH-L1 on cell migration. On the other hand, NOX4 overexpressed in HeLa cells happens to be ubiquitinated, and NOX4 following deubiquitination by UCH-L1, restored H2O2-generating activity. These in vitro findings are consistent with the results obtained in vivo with catalase (-/-) C57BL/6J mice. When H2O2 and UCH-L1 levels were independently varied in these animals, the former by infecting with H2O2-scavenging adenovirus-catalase, and the latter by overexpressing or silencing UCH-L1, pulmonary metastasis of B16F10 cells overexpressing UCH-L1 increased significantly in catalase (-/-) mice. In contrast, invasion did not increase when UCH-L1 was silenced in the B16F10 cells. These findings indicate that H2O2 levels regulated by UCH-L1 are necessary for cell invasion to occur and demonstrate that UCH-L1 promotes cell invasion by up-regulating H2O2 via deubiquitination of NOX4.

Setting: A South Indian district providing anti-tuberculosis services through the Revised National TB Control Programme (RNTCP) and private health care facilities., Objective: To ascertain the profile of tuberculosis (TB) patients diagnosed and/or treated in private health care facilities., Methods: Data on TB cases diagnosed and/or treated in all clinical departments of the medical college, 83 nursing homes and RNTCP health care facilities were collected prospectively., Results: About 83% of new TB cases recorded in the private medical college, 47% in nursing homes and 24.5% in RNTCP TB registers were extra-pulmonary. The proportion of retreatment cases was respectively 5.5%, 9.6% and 19.8%. The proportion of males and those in the economically productive age group were similar in the three data sources. About 94% of cases diagnosed in the medical college and 55% in nursing homes were registered for treatment under the RNTCP. About 11% of the smear-positive patients diagnosed in RNTCP were initial defaulters., Conclusion: The proportion of extra-pulmonary cases was higher in the medical college and nursing homes and that of retreatment cases was lower than in the RNTCP.

Background: For the diagnosis of smear-negative pulmonary tuberculosis (PTB), India's Revised National Tuberculosis Control Programme (RNTCP) recommends a course of broad-spectrum antibiotics after negative smear on initial sputum examination, followed by repeat sputum examination and chest X-ray (CXR)., Objectives: 1) To ascertain the proportion of presumptive PTB patients smear-negative on initial sputum examination who completed the diagnostic algorithm, and 2) to investigate barriers to the completion of the algorithm., Methods: In Karnataka State, India, 256 study participants were interviewed in 2012 to ascertain the number of days antibiotics had been prescribed and consumed, the number of re-visits to health centre(s), whether repeat sputum examinations had been performed, whether or not CXR had been performed and when, and whether PTB had been diagnosed. In-depth interviews were conducted with 19 medical officers., Results: The diagnostic algorithm was completed in 13 (5.1%) of 256 participants; three were diagnosed with PTB without completing the algorithm. Most medical officers were unaware of the algorithm, had trained 5-10 years previously, prescribed antibiotics for <10 days and advised CXR without repeat sputum examination, irrespective of the number of days of antibiotic treatment. Other main reasons for non-completion of algorithm were patients not returning to the health centres and a proportion switching to the private sector., Conclusion: Refresher training courses, raising patient awareness and active follow-up of patients to complete the algorithm are suggested.

Ocimum sanctum has been known to possess various beneficial properties including anti-oxidative, anti-inflammatory and anti-cancer activities. In the present study, we investigated that ethanol extracts of O. sanctum (EEOS) had anti-metastatic activity through activation of anti-oxidative enzymes. EEOS exerted cytotoxicity against Lewis lung carcinoma (LLC) cells. Also, EEOS significantly inhibited cell adhesion and invasion as well as activities of matrix metalloproteinase-9 (MMP-9), but not MMP-2, indicating the important role of MMP-9 in anti-metastatic regulation of EEOS. In addition, EEOS significantly reduced the tumor nodule formation and lung weight in LLC-injected mice. Inhibitory effect of EEOS on metastasis was further confirmed by using hematoxylin and eosin (H&E) staining. Notably, we also found that EEOS enhanced activities of anti-oxidative enzymes such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) in a concentration-dependent manner. Taken together, our findings support that EEOS can be a potent anti-metastatic candidate through inactivation of MMP-9 and enhancement of anti-oxidant enzymes., (Copyright 2010 Elsevier Ltd. All rights reserved.)

Objectives: In the pursuit of developing a second-generation oxazolidinone, we have identified OCID0050 as a novel oxazolidinone with enhanced activity against bacterial strains resistant to methicillin, vancomycin and linezolid., Methods: MIC and MBC determinations were performed according to CLSI guidelines. Linezolid-resistant bacterial strains were generated in-house; inoculum effect, pH effect and kill kinetics experiments were performed as per standard protocols., Results and Conclusions: OCID0050 demonstrated better inhibitory potency against many of the tested clinically significant strains by generally showing 2-4-fold lower MICs than linezolid. In addition, it has higher inhibitory activity against linezolid-resistant strains.

Although Ocimum sanctum has been used extensively for its medicinal values in India and China, its antitumor activity against human nonsmall cell lung carcinoma (NSCLC) A549 cells has not been investigated until now. Therefore, the antitumor mechanism of ethanol extracts of Ocimum sanctum (EEOS) was elucidated in A549 cells in vitro and the Lewis lung carcinoma (LLC) animal model. EEOS exerted cytotoxicity against A549 cells, increased the sub-G1 population and exhibited apoptotic bodies in A549 cells. Furthermore, EEOS cleaved poly(ADP-ribose)polymerase (PARP), released cytochrome C into cytosol and simultaneously activated caspase-9 and -3 proteins. Also, EEOS increased the ratio of proapoptotic protein Bax/antiapoptotic protein Bcl-2 and inhibited the phosphorylation of Akt and extracellular signal regulated kinase (ERK) in A549 cancer cells. In addition, it was found that EEOS can suppress the growth of LLC inoculated onto C57BL/6 mice in a dose-dependent manner. Overall, these results demonstrate that EEOS induces apoptosis in A549 cells via a mitochondria caspase dependent pathway and inhibits the in vivo growth of LLC, suggesting that EEOS can be applied to lung carcinoma as a chemopreventive candidate., ((c) 2009 John Wiley & Sons, Ltd.)

The objective of this investigation was to determine the efficacy of crocetin in preventing lung tumorigenesis in mice. We evaluated crocetin in Swiss albino mice treated with the tobacco-specific carcinogen benzo(a)pyrene [B(a)P] for their ability to inhibit pulmonary adenoma formation and growth. Male Swiss albino mice (7 weeks old) were given 100 mg/kg B(a)P by i.p. injection, and 4 or 14 weeks later, they were given crocetin 50 mg/kg by i.p. injection 3 days/week. Crocetin (50 mg/kg body weight) reduced proliferating cells by 68% and 45% in 18 and 8 weeks of treatment respectively. The levels of glycoproteins and polyamines were significantly altered in the B(a)P-induced animals than in crocetin treatment groups. The activity of crocetin was more pronounced in the cancer. Taken together, these results indicate that crocetin was capable of inhibiting proliferation cells by inhibiting proliferating cells, glycoprotein and polyamine synthesis., ((c) 2008 John Wiley & Sons, Ltd.)

Lithospermum erythrorhizon has been used for treatment of inflammatory diseases and cancer as a folk remedy. Based on the evidences that anti-inflammatory agents frequently exert antiangiogenic activity, thus we examined comparatively the antiangiogenic activities of three naphthoquinone derivatives (shikonin, acetylshikonin, and isobutyroylshikonin) isolated from the plant. Three derivatives exhibited weak cytotoxicity against human umbilical vein endothelial cells (HUVECs) with IC50 of over 20 microM. Shikonin had more specific inhibitory effects on proliferation and vascular endothelial growth factor (VEGF) production by VEGF compared with different derivatives. All of derivatives significantly suppressed the migration of VEGF treated HUVECs at different optimal concentrations. Also, shikonin and acetylshikonin significantly disrupted VEGF-induced tube formation. Furthermore, three derivatives effectively downregulated the expression of urokinase-type plasminogen activator (uPA), but not its receptor uPAR. Additionally, shikonin significantly inhibited tumor growth in LLC-bearing mice, whereas its derivatives had relatively mild effects. Taken together, our findings suggest that shikonin and its derivatives exhibit the antiangiogenic and antitumorigenic effects by suppressing proliferation and angiogenic factors.

Setting: Bangalore city slums, India., Objectives: To ascertain 1) health-seeking behaviour patterns in persons with pulmonary symptoms; 2) pathways followed by pulmonary tuberculosis (PTB) cases until diagnosis and treatment; and 3) their knowledge about TB-symptoms, cause, mode of transmission, diagnosis and treatment., Methods: In selected slums, persons with pulmonary symptoms identified during house visits and residents with PTB were interviewed using pre-tested, semi-structured questionnaires. Visits to relevant health centres were made to obtain information regarding their treatment., Results: About 50% of the 124 persons with pulmonary symptoms interviewed had taken action for relief; of these, three quarters had first approached private health facilities. About 19% had undergone sputum microscopy and 27% chest X-ray. Of 47 PTB cases interviewed, 72% first approached private health facilities; about 50% visited two health facilities before diagnosis and 87% visited two or more facilities before initiating treatment; 42 initiated treatment at government health facilities and five who initiated treatment at private health facilities were later referred to government health facilities. The majority of persons with pulmonary symptoms and PTB cases had poor knowledge about TB, and most of those with pulmonary symptoms were not aware of the availability of free anti-tuberculosis services at government health facilities., Conclusion: Educational interventions targeted at slum dwellers and their health providers are needed.

Chemoprevention has emerged as a very effective preventive measure against carcinogenesis. Several bioactive compounds present in fruits and vegetables have revealed their cancer curative potential on carcinogenesis. Tumor markers correlate strongly with prognosis on tumor burden. Glycoprotein and membrane ATPases play an important role in carcinogenesis. Hence this study was launched to evaluate the effect of mangiferin on the changes in glycoprotein components, ATPases and membrane lipid peroxidation in control and lung carcinoma bearing mice. A significant increase in the levels of glycoproteins, membrane ATPases and membrane lipid peroxidation were observed in animals with lung carcinoma. On administration of mangiferin, these changes were reverted back to near normal levels. The increased levels of glycoprotein components found in lung carcinoma were also significantly decreased in mangiferin treated. Overall, the above data shows that the anticancer effect of mangiferin is more pronounced when used as an chemopreventive agent rather than as a chemotherapeutic agent against B(a)P induced lung carcinogenesis., (Copyright © 2008 Elsevier B.V. All rights reserved.)

Carcinoma of the stomach is reportedly the second most common cancerous condition affecting the general population. Administration of antioxidants is reported to effectively alleviate the risk of gastric carcinoma. Therefore, we assessed the protective role of naringenin, an antioxidant and naturally occurring citrus flavanone, on gastric carcinogenesis induced by MNNG (200 mg/kg body weight) and S-NaCl (1 mL per rat) in Wistar rats (obtained from the Central Animal House Facility, University of Madras, Taramani Campus, Chennai, India). The animals were divided into 5 groups, and the effects of naringenin on simultaneous and posttreated stages of MNNG were tested. Cancer risk was analyzed along with their antioxidant status. The LPO levels in the experimental groups were assessed as an index of oxidative milieu. Altered redox status was subsequently investigated by assaying the superoxide and hydroxyl radicals, the enzymatic antioxidants (SOD, CAT, GPx), and the nonenzymatic antioxidants viz reduced GSH, vitamin C, and vitamin E. In the presence of MNNG, cancer incidence and LPO levels were significantly increased, whereas enzymatic (SOD, CAT, and GPx) and nonenzymatic antioxidant activities (GSH, Vitamins C, and E) were decreased in the treated rats compared with control rats. Administration of naringenin to gastric carcinoma-induced rats largely up-regulated the redox status to decrease the risk of cancer. We conclude that up-regulation of antioxidants by naringenin treatment might be responsible for the anticancer effect in gastric carcinoma.

Background: There is paucity of information on epidemiological situation of Tuberculosis (TB) in Andhra Pradesh. The DOTS strategy under the Revised National Tuberculosis Control Programme (RNTCP) was introduced in the year 2000 to cover the entire State by 2005., Objectives: To estimate the prevalence of tuberculous infection among children 5-9 years of age and to compute the average Annual Risk of Tuberculosis Infection (ARTI) from the estimated prevalence., Methods: A cluster-sample house-based tuberculin survey was carried out in a representative sample of children between 5-9 years of age. The clusters were selected by a two-stage sampling procedure. At first stage, five districts were selected by probability proportional to population size (PPS) method. Depending upon child population ratio, 32 clusters allocated to each district were further sub-divided into rural and urban clusters selected by simple random sampling. A total of 3636 children, irrespective of their BCG scar status, were tuberculin tested using one TU PPD RT23 with Tween 80 and the maximum transverse diameter of induration was measured about 72 hours later., Results: The prevalence of infection estimated by mirror-image technique using observed mode of reactions attributable to infection with tubercle bacilli at 20mm was 9.6% (95% CI: 8.0-11.2). The ARTI was computed at 1.4% (95% CI: 1.1-1.6)., Conclusion: Survey findings indicate a fairly high rate of transmission of tubercle bacilli.

The present study was aimed to evaluate the therapeutic effects of Withania somnifera along with paclitaxel on lung tumor induced by benzo(a)pyrene in male Swiss albino mice. The levels of ATPase enzymes and lipid peroxidation were evaluated in lung cancer bearing mice, in erythrocyte membrane and tissues. The extent of peroxidation was estimated by measuring the thiobarbituric acid-reactive substances. Simultaneously the activities of different ATPases (Na(+)/K(+)-ATPases, Mg(2+)-ATPases and Ca(2+)-ATPases) were determined. The alterations of these enzyme activities in membrane and tissues were indicative of the tumor formation caused by benzo(a)pyrene (50 mg/kg body weight, orally) in cancer bearing animals. The activities of these enzymes were reversed to near normal control values in animals treated with Withania somnifera (400 mg/kg b.wt, orally) along with paclitaxel (33 mg/kg b.wt, i.p). Treatment with Withania somnifera along with paclitaxel altered these damage mediated through free radicals, and the treatment displays the protective role of these drugs by inhibiting free radical mediated cellular damages. Over, based on the data providing a correlation Withania somnifera along with paclitaxel provide stabilization of membrane bound enzyme profiles and decreased lipid peroxidation against benzo(a)pyrene induced lung cancer in mice.

Lung cancer is one of the leading causes of cancer death in the world and is notoriously difficult to treat effectively. In the present study, male Swiss albino mice were divided into five groups of six animals each: group I animals received corn oil orally and served as a control; group II cancer-induced animals received benzo(a)pyrene (50 mg/kg bodyweight dissolved in corn oil, orally) twice weekly for four successive weeks; group III cancer-bearing animals (after 12 weeks of induction) were treated with paclitaxel (33 mg/kg bodyweight, i.p.) once weekly for 4 weeks; group IV cancer-bearing animals were treated with paclitaxel along with Withania somnifera (400 mg/kg bodyweight) orally once weekly for 4 weeks; and group V animals constituted the drug control treated with paclitaxel along with W. somnifera. The serum, lung and liver were investigated biochemically for aryl hydrocarbon hydroxylase, gamma-glutamyl transpeptidase, 5'-nucleotidase, lactate dehydrogenase and protein-bound carbohydrate components (hexose, hexosamine and sialic acid). These enzyme activities were increased significantly in cancer-bearing animals compared with control animals. The elevation of these in cancer-bearing animals was indicative of the persistent deteriorating effect of benzo(a)pyrene in cancer-bearing animals. Our data suggest that paclitaxel, administered with W. somnifera, may extend its chemotherapeutic effect through modulating protein-bound carbohydrate levels and marker enzymes, as they are indicators of cancer. The combination of paclitaxel with W. somnifera could effectively treat the benzo(a)pyrene-induced lung cancer in mice by offering protection from reactive oxygen species damage and also by suppressing cell proliferation.