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Plants of the Valeriana species are distributed in various parts of the world, especially in Europe and Asia. A high content of polyphenolic compounds, including flavonoids and hydroxycinnamic acids with expressed biological activity, was previously identified within the herb of the studied Valeriana species. Morphological and anatomical data can be used in phylogenetics of species and genera to find out diagnostic and age-related characteristics of plants. This led us to microscopic studies of aerial organs of the above-mentioned Valeriana species. The aim of the work is to conduct a comparative study of the diagnostic features within the morphological and anatomical structure of Valeriana stolonifera and Valeriana collina leaves. Materials and methods. Both raw and dried plant material of V. stolonifera and V. collina was used for microscopic studies. Temporary micropreparations were made using generally accepted methods. The anatomical features of the raw material were examined using Carl ZEISS “AxioStar Plus” and “Primo Star”. Results. The study identified key morphological and anatomical features of the species, which should be considered for the identification and standardization of promising medicinal plant materials and within the development of methods of analytical and regulatory documentation. Conclusions. The key microscopic differences in the leaves of the studied species lie in the structure of the adaxial and abaxial surfaces of the leaf blade. The upper epidermis consists of large elongate cells with wavy walls. The cells of the lower epidermis are smaller and have more sinuous walls compared to the upper epidermis cells. The degree of wall sinuosity varies between species – V. collina is characterized by more sinuous cells in both the upper and lower epidermis compared to V. stolonifera. The absence of stomata on the upper epidermis is a common feature for both species.

Myrtus communis L. is a promising medicinal plant that has garnered the attention of scientists to introduce it into industrial cultivation as a raw material source for obtaining new phytopreparations. In its chemical composition, Myrtus communis L. contains many biologically active substances, which confer upon it a range of medicinal properties including astringent, anti-inflammatory, antiviral, antibacterial, antifungal, antioxidant, antidiabetic, antiulcer, antimutagenic, gastro- and hepatoprotective properties. Published information primarily focuses on the chemical composition and pharmacological activity of common myrtle, with limited data available on its micromorphology. The aim of the work is to study the morphological and anatomical structure and to determine the general diagnostic microscopic features of common myrtle leaves. Materials and methods. The object of the research was fresh and dried plant material of common myrtle grown under room conditions. Transverse sections were fixed with Carnois fixative, dehydrated, and paraffinized. The sections were then cut using a Microm HM 325 microtome, followed by deparaffinization. Microscopic analysis of the leaf was performed using temporary preparations on a Carl Zeiss microscope, including the “AxioStar Plus” and “Primo Star”, equipped with a photo attachment for direct and reflected light microscopy. Results. The external features of common myrtle leaves include their shape, color, size, smell, and type of veining. Anatomically, the leaves exhibit a cuticle, dorsiventral mesophyll, and anomocytic stomata located on the abaxial surface. Additionally, they contain calcium oxalate crystals and druses, as well as simple hairs on the midvein and schizolisogenic secretory receptacles. Histochemical studies of these cavities indicate the presence of lipophilic substances. Conclusions. The morphological and anatomical analysis of Myrtus communis L., a representative of the myrtle genus, allowed for the identification of key diagnostic microscopic features of myrtle leaves. Further research prospects involve conducting a comprehensive set of phytochemical studies to establish standardization parameters for medicinal plant raw materials.

The aim of the work is to determine the component composition of the essential oil of medicinal chamomile, grown on the educational and research site of medicinal plants of Zaporizhzhia State Medical and Pharmaceutical University (ZSMPHU), and commercial essential oil that was purchased via the Internet. Materials and methods. The object of the study was chamomile essential oil obtained by hydrodistillation and commercial oil. Qualitative and quantitative determination of the components of essential oils was established using the chromato-mass spectrometric method on a high-performance gas chromatograph “Agilent 7890B GC System” (Agilent, SantaClara, CA, USA) with a mass spectrometric detector “Agilent 5977 BGC/MSD” (Agilent, SantaClara, CA, USA). Results. Dominant compounds of essential oil of chamomile, grown on the educational and research site of medicinal plants ZSMPHU were 2H-pyran-3-ol, tetrahydro-2,2,6-trimethyl-6-(4-methyl-3-cyclohexen-1-yl) ) – 7.54 %, chamazulene – 5.32 %, alpha-bisabolol – 4.30 %, 1H-cycloprop[e]azulen-7-ol, decahydro-3,1,7-trimethyl-4-methylene – 3.25 %, myrtenyl acetate – 3.03 %, azulene, 1,2,3,3a,4,5,6,7-octahydro-1,4-dimethyl-7-(1-methylethenyl) – 2.16 %, bisabolol oxide B – 1.86 %. Commercial chamomile essential oil was dominated by 3-methoxy-3-methylbutanol (56.15 %), diethyl phthalate (17.46 %), linalyl acetate (6.99 %), linalool (4.41 %). Conclusions. Industrial cultivation of chamomile in southern Ukraine shows a high biologically active substances content and can be in demand in the pharmaceutical industry’s consumer market for raw materials.

The number of medicinal products based on biologically active substances (BAS) of plant origin is increasing every year. Scientists are exploring non-official plant raw materials used in non-traditional medicine to expand the raw material base for creating new phytopreparations. Among numerous plants, common myrtle (Myrtus communis L.), which is a fairly common indoor plant, is recognized as a valuable source of BAS. The aim of the work is to analyze and summarize new literature data on the botanical characteristics, chemical composition, pharmacological and toxicological properties of Myrtus communis L. Materials and methods. Data were collected from several legitimate databases and services such as PubMed, Medline, Scopus, Google Scholar, ResearchGate using the key terms “Myrtus communis L.”, “botanical characteristics”, “pharmacological studies”, “phytochemical studies” in each database data Relevant and related data were filtered as appropriate if deemed relevant to the topic of interest. Time frames have also been adjusted to provide up-to-date information. Results. Myrtus communis L., commonly known as common myrtle, is a widely recognized medicinal plant in the Mediterranean region. Various parts of this plant, including the leaves, fruits, and flowers, have been used in traditional medicine for treating a wide range of ailments. The pharmacological properties of common myrtle are attributed to the presence of essential oils and phenolic compounds, and these properties include antimicrobial, antiviral, antidiabetic, antispasmodic, vasodilator, antiulcer, antioxidant, antitumor, and anti-inflammatory effects. This article offers an overview of the botanical characteristics and chemical composition of different parts of common myrtle, and it presents a literature review of pharmacological studies that provide evidence for the plant’s medicinal properties. Conclusions. Common myrtle is a promising plant for further pharmacognostic research with the aim of expanding the raw material base, using the method of clonal micropropagation and creating new phytopreparations based on it.

Valeriana officinalis L. s.l. is a perennial herbaceous plant belonging to the Valerianaceae family. It is widely distributed in Ukraine and around the world. The official medicinal raw material derived from Valeriana officinalis consists of the dried, whole, or fragmented underground parts of the plant, including the rhizome along with the roots and stolons. To meet the standards outlined in the European Pharmacopoeia, this raw material must be standardized based on its essential oil content (a minimum of 4 ml/kg in dried preparations) and sesquiterpenic acids (a minimum of 0.17 percent by weight, calculated as valeric acid, in dried preparations). The aim of the work was searching, systematizing, and generalizing information from the specialized literature on the chemical composition, biological activity and therapeutic use of Valeriana officinalis. Valeriana officinalis has sedative, mild analgesic, hypnotic, antispasmodic, carminative, and hypotensive effects. Historically, it was used for hysterical conditions, hyperexcitability, insomnia, hypochondria, migraine, spasms, intestinal colic, rheumatic pains, dysmenorrhea, and especially for conditions accompanied by nervous excitability. The main classes of biological substances identified in Valeriana officinalis are valepotriates, iridolactones, alkaloids, phenolic acids, sesquiterpenes, flavonoids, terpene coumarins, lignans, terpene and flavonol glycosides. It has not yet been established which components of Valeriana officinalis are responsible for its pharmacological effects. Current opinion is that the overall effect of valerian is due to several different groups of components and their different mechanisms of action. Therefore, the activity of different preparations of valerian will depend on their content and concentration of several types of components. Conclusions. Summarized and systematized original works related to pharmacognostic study, phytochemical analysis, and medicinal use of underground and aerial parts of various species of Valeriana genus. The analysis of the material shows that in recent years, scientists from all over the world have paid attention to the above-ground part of valerian and have chosen it as an object of in-depth study. This approach opens new possibilities for the use of the herb Valeriana officinalis in medicine.

Derivatives of 1,2,4-triazole open wide opportunities for modern and progressive scientists in the development of innovative medicines. These compounds are known for their variability and structural flexibility, which allows scientists to experiment and create new molecules with unique properties. The use of 1,2,4-triazole derivatives in the creation of drugs is based on their ability to interact with biological systems and molecular targets. These compounds can be aimed at regulating physiological processes, reducing manifestations of pathological conditions or enhancing necessary biological reactions. Directed modification of the structure of 1,2,4-triazole derivatives allows to create biologically active compounds with improved properties. The aim of the work was to study in silico and to evaluate the possible interaction of a virtual series of S-alkyl derivatives of 5-methyl-4-(p-tolyl)-1,2,4-triazole-3-thiol with some enzyme systems. Materials and methods. A computer method (molecular docking) for predicting and evaluating the interaction between a ligand molecule and a target protein structure. Ligand preparation was performed using MarvinSketch 6.3.0, Hyper Chem 8, and AutoDockTools-1.5.6 programs. Enzyme preparation involved the use of Discovery Studio 4.0 and AutoDockTools-1.5.6 software packages. Direct molecular docking was performed using the Vina program. Results. A virtual series of S-alkyl derivatives of 5-methyl-4-(p-tolyl)-1,2,4-triazole-3-thiol with the potential possibility of creating a biologically active substance has been constructed. Using the Vina software tool, the nature and number of amino acid residues of the active centers of model enzymes, with which the proposed ligands coordinate and bind, were determined. According to the results of docking studies, the predicted affinity for lanosterol-14α-demethylase was determined. The effect on the receptor tyrosine kinase of anaplastic lymphoma is somewhat inferior in terms of qualitative and quantitative indicators. Conclusions. Using the method of molecular docking, it was established that S-alkyl derivatives of 5-methyl-4-(p-tolyl)-1,2,4-triazole-3-thiol have a fairly significant potential for the manifestation of antifungal activity, which justifies the further synthesis of these compounds and more in-depth study of fungistatic and fungicidal properties. Docking results for anaplastic lymphoma kinase show little promise in the development of anticancer agents.

The main direction of the development of the education system in Ukraine is the introduction of European standards, technologies, norms, and perspectives to ensure competitiveness in the market of European education services. The study aimed to highlight modern approaches to the standardization of higher education in the context of shaping professional competences of future pharmacists, to identify peculiarities and possibilities of applying a systemic approach to the development of a higher education standard for the specialty of 226 Pharmacy, Industrial Pharmacy of the second (master’s) level. The research materials consisted of references containing information on the development, approval, and implementation of higher education standard. The following methods were used in the study: synthetic, analytical, information search, descriptive, and generalization. The pharmaceutical industry is socially significant, and the specialty of 226 Pharmacy, Industrial Pharmacy, as well as all the specialties in the field of study 22 Health, are regulated. Therefore, professionals in this field should possess integrated knowledge and skills, be able to solve complex tasks and problems in the field of pharmacy and healthcare, both in practice and during the learning process. This involves conducting research, implementing innovations, and is characterized by uncertain conditions and requirements. A modern approach also involves the use of highly efficient technologies in the national and international educational and scientific environments, which ensure the preparation of competitive, highly qualified pharmaceutical professionals with moral and spiritual values, social responsibility, and community orientation. They are capable of providing quality pharmaceutical care to people and possess «soft skills». Upon the development of the approved higher education standard for the specialty of 226 Pharmacy, Industrial Pharmacy by the Ministry of Education and Science of Ukraine, the educational and professional programs Pharmacy and Perfume and Cosmetic Technologies were revised, and a list of competences as well as program learning outcomes for future pharmaceutical professionals was specified. These were aligned with the demands of the labor market and a professional-oriented approach, based on the concept of competences for a modern pharmacist and the variable components of educational and professional training programs

The aim of this study was to establish the component composition of the freon extract of Damask rose grown in vitro and to substantiate the advantages of this method and the prospects for the use of phytosubstances in medical practice. Materials and methods. The object of the study was the freon extract of Damask rose, grown by the method of clonal micropropagation in vitro. The qualitative and quantitative determination of the components of the freon extract was determined using the chromato-mass spectrometric method. Results. According to the results of the chromato-mass-spectrometric study, 60 compounds were identified, 6 of which were in the isomeric state. The following components prevailed by percentage: рhenylethyl alcohol – 64.070 %, citronellol – 6.090 %, nonadecane – 4.636 %, heneicosane – 2.590 %, geraniol – 1.749 %. Conclusions. The chemical composition of the freon extract of Rosa damascena Mill., grown in vitro, was investigated for the first time using the chromato-mass-spectrometric method. The main components of rose petals: рhenylethyl alcohol – 64.070 %, citronellol – 6.090 %, nonadecane – 4.636 %, heneicosane – 2.590 %, geraniol – 1.749 %.

Modern sedatives used in medical practice can be of both synthetic and natural origin. It is known that the best phytosedative is valerian. In the 18th century, valerian was included in all European pharmacopeias. High efficiency, good tolerability, and virtually no side effects ensure the widespread use of valerian and its phytopreparations in medical practice, especially for the treatment of geriatric patients. Despite this, valerian remains understudied due to its extreme polymorphism. The aim of the work is to determine the component composition of valerian tinctures made from rhizomes with valerian roots of different species growing in the Zaporizhzhia region by gas chromatography and to carry out their comparative analysis. Materials and methods. Samples of rhizomes with valerian roots made from medicinal plant raw materials of valerian according to the traditional production recipe (Tinctura Rhizomata cum radicibus Valerianae (1:5)) from different species of valerian growing in the Zaporizhzhia region were selected for experimental studies: Valerian stolonifera Czern. – Kantserivska Balka, Zaporizhzhia district, Zaporizhzhia region; V. exaltata Mikan. – Shyroke village, Vasylivskyi district, Zaporizhzhia region; V. tuberosa L. – Khortytsia Island, Zaporizhzhia; V. collina Wallr. – right bank of the Dnieper River, Zaporizhzhia. The component composition of valerian tinctures was studied using an Agilent 7890B gas chromatograph with a mass spectrometric detector 5977B. Results. The component composition of tinctures from the underground part of valerian plants growing in the Zaporizhzhia region was identified and analyzed with chromato-mass spectrometry. Conclusions. 55 components in valerian tincture from underground raw materials of Valeriana stolonifera Czern. were identified with gas chromatography: V. exaltata Mikan. – 48, V. tuberosa L. – 54, V. collina Wallr. – 51. Analyzing the obtained data, we can conclude that valerian tinctures differ in both quantitative and qualitative composition. Only compounds 7 and 9 coincide in tinctures from underground parts of all four types of valerian. Since the tinctures were made following the standard technology, the content of components in medicinal plant raw materials depends on the type, place of growth, environmental conditions, time of collection, drying of raw materials. The results of the study confirm the prospects of using the underground part of the studied plants of the genus Valerian to create new drugs and phytopreparations on their basis. However, given the extreme polymorphism of valerian for the introduction into the culture of promising species that grow in Ukraine, it is necessary to conduct more in-depth pharmacognostic and pharmacological studies of plants of this genus.

Valerian Officinalis (Valeriana officinalis L.s.p.) is a collective species in Ukraine. It contains 13 species, including in the south of Ukraine grows valerian (V. stolonifera Czern.). At the present stage, it is confirmed that the calming and antispasmodic properties of valerian raw materials are due to the content of valepotriates, sesquiterpenoids and aromatic substances, in particular eugenol derivatives. That is why in many countries around the world a number of drugs have been created on their basis. The aim of our research is to determine the component composition of tinctures of tinctures of V. stolonifera Czern. made of 70 % alcohol and methanol using gas chromatography. Materials and methods. Raw materials (underground organs) Valerianae radices were harvested in March 2020 in Zaporizhzhia, Kantserivska Balka. Valerian tincture was prepared from fresh raw materials according to the method of making tinctures. The tincture was investigated on a gas chromatograph Agilent 7890B with mass spectrometric detector 5977B. The NIST14 mass spectrum library was used to identify the components. Results. 77 components were identified by gas chromatography in methanolic tincture of V. stolonifera Czern. roots, 13 components were isolated quantitatively. In the analysis of the chromatogram and the characteristic of the area of the peaks of the alcoholic tincture of V. stolonifera Czern. roots, 78 components were identified, 11 components were isolated quantitatively. At the comparative characteristic of chromatograms 39 components coincide. Of the main components, the largest content is: 13.413 RT methanolic 1(2H)-Naphthalenone, octahydro-4a,8a-dimethyl-7-(1-methylethyl)-, [4aR-(4a.α.,7.β.,8a.α.)] – 5.34 % 13.414 RT alcohol – 8.41 %; 11.943 RT methanolic Myrtenyl isovalerate – 3.88 %; 11,942 RT alcohol – 5.15 %; 15.362 RT methanolic Kessanyl acetate – 2.52 %; 15.360 RT Kessanyl acetate alcohol – 1.41 %. Conclusions. It was seen that valerian tinctures with methanol and alcohol extractant differ in both quantitative and qualitative composition analyzing the obtained data of gas chromatography. 39 Compounds were present in all tinctures; three of them were present in large quantities. According to the results of the study, data have been obtained that show that the best indicators of the quantitative and qualitative composition of the tincture of V. stolonifera Czern. were obtained using the extractant 70 % alcohol.

The family Apiaceae includes 474 genera and 3992–4050 species that are distributed around the globe. Of all the variety of species, our attention was drawn to the raw material of anise – Anisum vulgare Gaertrn.; fennel – Foeniculum vulgare Mill., which contains derivatives of phenylpropane; coriander – Coriandrum sativum L.; dill – Anethum graveolens L.; caraway – Carum carvi L., which is rich in monoterpenoid derivatives. These plants are widely used in medical practice and known to have antimicrobial, antispasmodic, expectorant, antihypertensive, diuretic, choleretic, carminative effects. This versatility is due to the presence of different groups of biologically active substances in the raw materials of plants. The aim of the research is to determine, by means of gas chromatography, a component composition of fruit tinctures of Anisum vulgare Gaertrn., Foeniculum vulgare Mill., Coriandrum sativum L. Anethum graveolens L. and Carum carvi L. Materials and methods. Tinctures were prepared in ratio 1:5 (extractant – 70 % ethyl alcohol) from anise, fennel, coriander, dill, and caraway fruits, which had been harvested in the experimental field of Zaporizhzhia State Medical University in August 2019. Qualitative and quantitative determination of the active compounds was performed using an Agilent 7890B gas chromatograph with a 5977B mass spectrometric detector. The NIST14 mass spectrum library was used to identify the components. Results. It has been revealed that the tinctures of Anisum vulgare Gaertrn., Foeniculum vulgare Mill., Coriandrum sativum L. Anethum graveolens L., and Carum carvi L. differ in qualitative and quantitative composition of components of chromato-mass spectrometry. Tinctures of Anethum fructus contain 28 components, among which 8.028 RT (-)-Carvone (52.63 %) and 4.861 RT D-Limonene (17.74 %) prevail. Tinctures of Coriandrum fructus contain 37 components with the highest content of 11,016 RT Benzene, 1-(1,5-dimethyl-4-hexenyl)-4-methyl (20.7 %) and 19.372 RT 1-(4-hydroxy-3-methoxyphenyl)dec-4-en-3-one (8.57 %). In the tincture of Carum carvi L., which contains 33 components, the following may be considered predominant: 7.988 RT Benzaldehyde, 4-(1-methylethyl) (47.37 %) and 19,311 RT undetermined compound (5.88 %). The tincture of Anisum fructus has 22 components, with the highest content of 8.531 RT Estragole (60.32 %) and 4.852 RT D-Limonene (5.15 %); Foeniculum fructus tincture contains 52 components, among which 5.745 RT Fenchone (15.39 %) and 8.5 RT Anethole (11.37 %) prevail. Conclusions. The results of gas chromatography of fruits of the Apiaceae family significantly expand the information about the chemical composition of raw materials and indicate the prospects for use in pharmaceutical and medical practice. Analyzing the results of gas chromatography, it has been found that tinctures of fruits of the Apiaceae family differ in qualitative and quantitative composition of components. Tinctures of Foeniculum fructus contain 52 components, Coriandrum fructus – 37 components, Carum carvi L. contain 33 components, Anethum fructus – 28 components, Anisum fructus – 22 components. The obtained experimental data can be used to create regulatory documentation for a particular type of medicinal plant raw materials.

Underground Valerian roots (Vаlеrіаnае radix) are officinal raw materials. More than 1000 compounds were detected in Valeriana officinalis L. s.l., of which no less than 500 natural substances of organic and inorganic origin were identified, among them – carbohydrates, amino acids, lipids, vitamins, enzymes, phenolic compounds, essential oil, iridoids, alkaloids, steroids. The aerial part of Valerian is a source of various flavonoids with pronounced antioxidant properties and specific pharmacotherapeutic activity. The aim of the research is to determine, by means of gas chromatography, component composition of Valerian tinctures, made of the aerial part of V. tuberosa L., V. stolonifera Czern (Kantserivska balka, Zaporizhzhia region, Ukraine) and V. officinalis L. (Łódź, Bydgoszcz, Poland). Materials and methods. Tincture samples of the aerial part of wild species V. tuberosa L., V. stolonifera Czern (Kantserivska balka, Zaporizhzhia region), and V. officinalis L. (the experimental site of ZSMU; Łódź and Bydgoszcz in Poland) were selected for experimental studies according to the traditional production recipe for making tinctures (1 : 5). Component composition of the tinctures was studied using a 7890B gas chromatograph with a 5977B mass spectrometer detector. Results. By means of chromato-mass spectrometry, component composition of the Valerian tinctures has been identified, namely: in the tincture prepared from the aerial part of V. officinalis (Łódź, Poland) 54 components have been identified, V. tuberosa – 50; V. stolonifera – 46 and V. officinalis (Bydgoszcz, Poland) – 30. The chromatogram analysis of four Valerian tinctures helped to identify 118 components, 5 of which were present in all studied tinctures; 12 components were present in three tinctures; 22 components have been found in two tinctures. It should be noted that 10 components have been found in the tinctures in large quantities, their content ranging from 10.91 % to 30.02 %. Conclusions. Analyzing the obtained data of the GC, it can be seen that Valerian tinctures differ both in quantitative and qualitative composition. From the aerial part of Valeriana officinalis, prepared in Łódź and Bydgoszcz in Poland, 17 components coincide. In the tinctures, prepared from herbs collected in Ukraine, 10 components coincide. The highest content of Butanoic acid, 3-methyl – from 4.01 % to 16.78 %; 9,12,15-Octadecatrienoic acid, ethyl ester, (Z,Z,Z)- – from 0.47 % to 7.09 %; Dihydroxyacetone – from 0.37 % to 5.69 % and Quinic acid – from 0.30 % to 4.66 % in all four tinctures by peak areas and retention time should be marked. In three tinctures Cyclododecane, 1,5,9-tris(acetoxy)- has been identified in amount of 30.56 % to 14.56 %. The obtained results confirm the expediency of using the aerial part of Valeriana officinalis as raw material with a large commodity yield and various contents of biologically active substances for further pharmacognostic researches for the purpose of creation of new medicines and phytopreparations

Motherwort has a cardiotonic effect – slows the heart rate and increases the strength of heart contractions, antihypertensive – lowers blood pressure, has a calming and antispasmodic effect in disorders of the cardiovascular system: in the early stages of hypertensive heart disease, mild forms of angina pectoris, heart defects and Graves' disease. Red clover tincture is used for atherosclerosis, which is accompanied by headaches and tinnitus, but with normal blood pressure; it provides detoxification of the liver and the body as a whole by cleansing blood and lymph; improves bile flow; normalizes bowel activity. The purpose of the work is to determine, by means of gas chromatography, the component composition of red clover and motherwort tinctures. Materials and methods. Red clover grass (Trifolii pretense herba) was collected in Zakarpattia, Chynadiyovo village, in July 2019; motherwort grass (Leonuri herba) was collected on the experimental site of ZSMU in June 2019. The tinctures were prepared from fresh raw materials (1:5) by maceration, using 70 % ethanol as the extractant. The tinctures were examined using an Agilent 7890B gas chromatograph with a 5977B mass spectrometer detector. Results. 20 Components were identified with gas chromatography in red clover tincture, of which: 1 aldehyde, 2 ketones, 3 esters, 3 heterocyclic compounds, 3 acids, 4 alcohols, and 4 aliphatic carbohydrates. In the motherwort tincture 30 characteristic components were identified, related to: organic acids (3 compounds); ketones (4); esters (6); aliphatic carbohydrates (1); alcohols (2); heterocyclic compounds (2); glycosides (1); nitrogen-containing compounds (1); aromatic compounds (3); sesquiterpenoids (3); phenolic compounds (2); aldehydes (1); undetermined compounds (2). Conclusions. It was used a gas chromatograph with a mass spectrometric detector in this work. 30 components were identified in motherwort tincture, of which, considering the quantitative analysis of the total peak area and retention time, the following should be distinguished: RT 16.26 – n-hexadecanoic acid – 15.2 %; RT 17,695 – phytol – 13.66 %; RT 12,835 – ethyl-d-glucopyranoside –10.99 %; RT 6.451 – 4H-pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl – 3.98 %; RT 20.7581 – penten-3-one, 1-(2,6,6-trimethyl-1-cyclohexen-1-yl) – 3.42 %; RT 10.43 – benzaldehyde, 2-hydroxy-6-methyl – 3.04 %; RT 3.664 –1,2-cyclopentanedione –1.22 %. In red clover tincture 20 components were identified, of which, considering the quantitative content, the following should be distinguished: RT 13.921 – myo-inositol, 4-C-methyl – 50.03 %; RT 12.742 – ethyl-d-glucopyranoside – 4.83 %; RT 22,435 – γ-sitosterol – 3.84 %; RT 16.261 – n-hexadecanoic acid – 3.72 %; RT 17,696 – phytol – 1.56 %; RT 8.764 – ethanone, 1- (2-hydroxy-5-methylphenyl) – 1.39 %. The two components coincide – n-hexadecanoic acid and phytol. In quantitative terms, there were more of them in the motherwort tincture. The results of the study can be used to develop the technology of herbal preparations, which include the raw material of red clover and motherwort. The study also has practical importance in the identification of raw materials that are included in the herbal products.

Valerian (Valeriana officinalis L.s.l.) is a flowering plant that includes 13 species in Ukraine. Valeriana stolonifera Czern is the most common of southern species that can be found in the Zaporizhzhia region. The duration and effectiveness of its drying depend on morphological features of Medicinal Herb Raw Material (MHRM), reference humidity, total surface area, thickness and lignification of the roots. Due to the process of dehydration as a result of drying, the increase of active substances in MHRM is sometimes observed. This process is conditionally called a “ripening stage”, or fermentation. The purpose of the work is to determine, by means of gas-liquid chromatography, the component composition of valerian tinctures made from fresh roots of Valeriana stolonifera Czern and dried with different periods of drying (7 days, 14 days and 21 days). Materials and methods. The tinctures were prepared according to production procedure (Tinctura Rhizomata cum radicibus Valerianae (1 : 5) (The extractant is ethanol, 70 %) made from the roots of Valeriana stolonifera Czern, which were harvested in November 2019 in the vicinity of Zaporizhzhia region (Kantserivska balka). Component composition of the Valeriana tinctures was investigated using the gas chromatograph Agilent 7890B with a 5977B mass spectrometry detector. Results. By means of chromatic-mass spectrometry in the valerian tinctures from raw materials, 49 component compositions were identified. After 7 days of drying, 83 components were identified; after 14 days – 95 components; after 21 days – 95 components. The qualitative composition showed that 25 components were present in all of the investigated tinctures. Some amount of components increased in number during the process of drying . The result of the researches proves that the level of valeric acid in the raw material was 1.84 %; after 7 days of drying – 2.65 %; after 14 days – 3.21 %; after 21 days – 3.72 %. Conclusions. Analyzing the obtained data of the GLC it can be seen that valerian tinctures differ both in quantitative and qualitative composition. 25 compounds were present in all investigated series, 6 of which are present in large numbers. Since the tinctures were made in one technology, the content of essential oils in the medicinal plant depends not only on species, place and time of collection, ecology and climate latitudes but in a significant way on drying of raw materials. Given the extraordinary polymorphism of valerian for the introduction into the culture of promising species growing in Ukraine, it was necessary to conduct deeper pharmacognostic and pharmacological studies. Experimental data obtained in the process of the research can be used for creating regulatory and analytical documentation for a certain type of raw materials and were of undoubted practical importance.

Underground organs of Valeriana Officinalis are part of multicomponent phytocompositions, which allows them to vary the content and, thus, intensively affect various links of pathological disorders in the body. Despite this, valeriana drug remains poorly understood because of its extraordinary polymorphism. The purpose of the work is to determine, by means of gas-liquid chromatography, the component composition of valerian tinctures produced by PrAT Pharmaceutical Factory “Viola”, made from rhizomes with valerian roots from different suppliers / manufacturers and various series. Materials and methods. For experimental studies we took samples of tinctures of rhizomes with valerian roots produced by PRAT Pharmaceutical Factory Viola, made from medicinal plant raw materials of valerian from different suppliers / producers, namely: from firm “Herbar Sp.zoo”, Warsaw, Poland and from firm LLC “Fitosvit Ltd”, Vinnitsa region, Ukraine according to the traditional formula (Tinctura Rhizomata cum radicibus Valerianae (1: 5). Component composition of the valeriana tinctures was investigated using the gas chromatograph Agilent 7890B with a 5977B mass spectrometry detector. Results. The component composition the of the valerian tinctures was identified by chromatography-mass spectrometry. In the analysis of the chromatogram of the four valerian tinctures series, 75 components were identified, 25 of which were present in all series of studied tinctures. It should be noted that six compounds (3, 14, 36, 40, 47, and 52) in all series are present in large quantities. Among them valerian acid (compound 47), the content of which in three series is almost at the same level of 3.1–3.72 %. The smallest amount is contained in the tincture of valerian from the raw materials of LLC "Fitosvit Ltd" 150518 series and is 1.54 %, and the biggest amount in the tincture by firm "Herbar" series 040118–3.72 %. Conclusions. Analyzing the obtained data of the GLC it can be noted that valerian tinctures differ both on quantitative and qualitative composition. 25 compounds are present in all investigated series, 6 of which are present in large amounts. Since the tinctures were made in one technology, the content of essential oils in the medicinal plant depends by the place, time of collection, drying of raw materials, ecology, climatic latitudes. Deeper pharmacological and pharmacological research is needed on the territory of Ukraine.

Today, an increase of natural and technogenic situations leads to the disorders of the central nervous system, functional-metabolic processes, vascular diseases, in particular, acute cerebral blood flow disorders. In addition, the changes occurring on the molecular and cellular levels are in the basis of the functional violations of individual systems and the organism as a whole. Hypoxia not only complicates the disease course, but in most cases, determines its outcome. The important role in the fight against hypoxia belongs to antioxidants, which improve the circulating oxygen utilization by the body, reduce its need for the organs and tissues, which is not only expedient but necessary for the treatment of many acute and chronic pathological processes. So, the frequency of the hypoxic states and a wide range of factors causing them determine the relevance of new ways and methods finding to overcome the oxygen deficiency. The aim of this work is the purposeful search of some 5-[((5-(adamantane-1-yl)-4-R-4H-1,2,4-triazole-3-yl)thio)methyl]-N-R1-1,3,4-thiadiazole-2-amines and 5-[((5-(adamantane-1-yl)-4-R-4H-1,2,4-triazole-3-yl)thio)methyl]-4-R1-4H-1,2,4-triazole-3-thiols, the study of their physical and chemical properties and pharmacological screening of the antihypoxic activity of the obtained compounds. Materials and methods. The study of physical and chemical properties was conducted on certified and licensed modern equipment. Antihypoxic activity was studied during the modeling process of hypoxia with hypercapnia. Mexidol was used as a comparison drug in studies at a dose of 100 mg/kg. Results. As the result of the study, it was found that the synthesized compounds and the comparison drug influenced on rats’ life span differently. Compounds, the antihypoxic activity of which exceeded control have been discovered, and others’ were at the level of Mexidol. A number of compounds showed a somewhat less activity in comparison with control, and two compounds, among the investigated ones were weaker then comparison drug action, but they did not reduce the life expectancy of the investigated groups in comparison with control. The effect of the remaining compounds on the lifetime of the investigated group was at the level of control group that received the physiological solution. Conclusions. A series of new compounds of derivatives among 5-[((5-(adamantane-1-yl)-4-R-4H-1,2,4-triazol-3-yl)thio)methyl]-N-R1-1,3,4-thiadiazole-2-amines and 5-(5-(adamantane-1-yl)-4-R-4H-1,2,4-triazole-3-yl)thio)methyl)-4-R1-4H-1,2,4-triazole-3-thiols have been synthesized, the structure of which has been confirmed with the help of modern physical and chemical methods of analysis. The most active compounds among the studied ones were 3a and 2a, their antihypoxic activity exceeds control on 37.86 % and 36.30 % and Mexidol is on 7.35 % and 5.79 % respectively.

Special attention of pharmaceutical scientists is attracted to the creation of new potential substances. They select natural heterocyclic molecules that exhibit various types of pharmacological activity as basic structures. Adamantane derivatives, as well as a number of other framework compounds, in the vast majority are biologically active substances. This fact causes interest to the given classes of organic compounds as potential sources for the development of new drugs. Due to the high pharmacological action of 1,2,4-triazole derivatives, the combination of adamantane and 1,2,4-triazole in one molecule can lead to the formation of high-level pharmacological substances. The aim of this work is the synthesis of some 2- (2 - ((5- (adamantane-1-yl) -4-R-1,2,4-triasole-3-yl) thio) acetyl) -N-R1-hydrazine-carbothioamides, study of their physical and chemical properties, antimicrobial and antifungal activity. Materials and methods. The chemical names of the compounds are given in accordance with the requirements of the IUPAC nomenclature (1979) and the recommendations of the IUPAC (1993). Study of the physical and chemical properties of 2-(2-((5-(adamantane-1-yl)-4-R-1,2,4-triazole-3-yl)thio)acetyl)-N-R1-hydrazine-carbothioamides have been carried out on the certified and licensed equipment in the ZSMU physical-chemical laboratories. The melting temperature was determined by an open capillary method on the Opti Melt MPA 100. The elemental composition of the synthesized compounds was determined on the universal analyzer Elementar Vario L cube (CHNS) (standard – sulfanilamide). 1H NMR spectra were recorded on a spectrometer Varian Mercury VX-200 (1H, 200 MHz) in dimethylsulfoxide-d6solvent (tetramethylsilane - internal standard) and are decoded using ADVASP(tm)Analyzer program (Umatek International Inc.). Chromatographic mass spectral studies were performed on a gas-liquid chromatograph Agilent 1260 Infinity HPLC equipped with an Agilent 6120 mass spectrometer (ionization in an electric spray (ESI). Results. New 2-(2-((5-(adamantane-1-yl)-4-R-1,2,4-triazole-3-yl)thio)acetyl)-N-R1-hydrazine-carbothioamides are firstly synthesized, their structure is established by means of modern physical chemical methods of analysis (elemental analysis, IR-, NMR 1H-spectroscopy), and their individuality is done by HPLC-MS method. In all cases, the meanings of the investigated physical and chemical constants coincide with the estimated theoretical indicators. Determination of antimicrobial and antifungal activity was carried out by 2-fold serial dilutions method in liquid nutrient media. As a result of conducted investigations the compounds which levels of antimicrobial and antifungal action approaching, and in some cases exceeding comparison drug trimethoprim were revealed among the synthesized compounds. Conclusions. A compound of 2-(2-((5-(adamantane-1-yl)-4-phenyl-1,2,4-triazole-3-yl)thio)acetyl)-N-ethyl-hydrazine-carbothioamide occurred as the most active in relation to all test-strains among the synthesized substances, the force of antimicrobial and antifungal activity of it several times exceeds the comparison standard trimethoprim.

Introduction. The compound adamantane-1-ammonium 2-((5-(adamantane-1-yl)-4-phenyl-4H-1,2,4-triazole-3-yl)thio)acetate was first synthesized at the Department of Toxicology and Inorganic Chemistry (Zaporizhya State Medical University), and received the name “Adatryn”. According to pharmacological studies, it shows neuroleptic properties and can be used in pill form, as most neuroleptics. Thus it is important to develop pills technology of adamantane-1-ammonium 2-((5-(adamantane-1-yl)-4-phenyl-4H-1,2,4-triazole-3-yl)thio)acetate. During pharmacological studies the optimum dose at one time was 70 mg. The aim of this work was to study the effect of different groups of excipients on pharmaco-technological properties of adamantane-1-ammonium 2-((5-(adamantane-1-yl)-4-phenyl-4H-1,2,4-triazole-3-yl) thio) acetate tablets. Materials and methods. In the process, we studied four groups of adjuvants, which have various physical and technological properties. In the study of four qualitative factors we used one of dispersion analysis plans – four-factor experiment based on hyper-Greco-Latin square. 16 Excipients were studied, most of which have appeared on the market in recent years and there is no information about their use in pharmaceutical technology in creation of tablet dosage forms. In the experiments we used modern equipment to determine the mass uniformity of drugs, abrasion (abrasion device to determine Pharmatest D-63512 Hainburg, Germany), the time of disintegration (ERWEKA D-63150, Germany), and drugpres (Belorussia). Results and discussion. Given pharmaco-technological properties and the results of morphometric studies proposed a method of wet granulation to obtain adamantan-1-ammonium 2-((5-(adamantan-1-yl)-4-phenyl-4H-1,2,4-triazole-3-yl)thio)acetate tablets. Conclusions. The influence of four groups of auxiliary substances on the technological characteristics of the adamantan-1-ammonium 2-((5-(adamantan-1-yl)-4-phenyl-4H-1,2,4-triazole-3-yl)thio)acetadote tablets, has confirmed the possibility of their obtaining by method of direct pressing. Optimal excipients and the composition of the drugs have been chosen.

The nitrogen-containing heterocyclic systems from the class of 1,2,4-triazole derivatives, which exhibit wide range of actions, occupy special place among the variety of heterocyclic compounds. Derived biologically active substances are actively introduced into practice as new original and effective drugs. We were interested in esters of 2-((5-(adamantan-1-yl)-4-R-4H-1,2,4-triazole-3-yl)thio)acetic acids, which exhibit high biological activity and can be intermediates for the synthesis of amides, hydrazides, ylidenderivatives of corresponding acids. The aim of this work is the synthesis of new esters of 2-((5-(adamantan-1-yl)-4-R-4H-1,2,4-triazole-3-yl)thio)acetic acids and the establishment of their physical-chemical properties. Materials and methods. Melting point was determined by open capillary method on the device OptiMelt MPA100. The elemental composition of the synthesized compounds was determined on the universal analyzer ElementarVario ЕL cube (CHNS) (standard – sulfanilamide). 1H NMR spectra were recorded on spectrometer Varian Mercury VX-200 (1H, 200 MHz) in the solvent dimethyl sulfoxide-d6 (tetramethylsilane internal standard) and decoded using a program ADVASP(tm) Analyzer program (Umatek International Inc.). Chromato-mass-spectral studies were performed on hazarding chromatograph Agilent 1260 Infinity HPLC equipped with mass spectrometer Agilent 6120 (ionization electro-spray (ESI). The results and discussion. Synthesis of 11 new compounds, namely esters of 2-((5-(adamantan-1-yl)-4-R-4H-1,2,4-triazole-3-yl)thio)acetic acids was carried out by two methods. According to the A method the alkylation of previously synthesized 3-(adamantan-1-yl)-1H-1,2,4-triazole-5-thiol was performed with the use of corresponding methyl ester of 2-chloroacetic acid and the presence of equivalent amount of sodium hydroxide. The B method involves the etherification of 2-((5-(adamantan-1-yl)-4-R-4H-1,2,4-triazole-3-yl)thio)acetic acid with the use of methyl, ethyl, i-propyl, n-propyl and n-butyl alcohols in the presence of concentrated sulfuric acid. Compounds, which were obtained by different methods, do not give depression of the melting point. It should be noted that the practical yield of the reaction according to the A method is a little bit higher in comparison with the B method. Conclusions. The study of 11 new synthesized compounds, namely esters of 2-((5-(adamantan-1-yl)-4-R-4H-1,2,4-triazole-3-yl)thio)acetic acids has been performed. The structure of the synthesized compounds has been confirmed by modern instrumental methods of analysis (1H NMR spectroscopy, IR spectrophotometry, gas chromatography-mass spectrometry and elemental analysis).

Lately adamantine containing substances have found wide use among the skeleton derivatives. The combination of adamantane and 1,2,4-triazole in one molecule creates significant preconditions to design new potential drugs with low toxicity and pronounced pharmacological activity. The aim of the research is the study of antimicrobial and antifungal activity of (5-(adamantane-1-yl)-4-R-1,2,4-triazole-3-ylthiols substitutes. Materials and methods. Determination of antimicrobial and antifungal activity has been carried out by 2-fold serial dilutions method in liquid nutrient media. The substance of the antibacterial drug Trimethoprim has been applied as a control in determining antimicrobial activity of the compounds against the investigated strains of microorganisms. Results. According to the data of experiments the 5-(((5-(adamantane-1-yl)-4-ethyl-4H-1,2,4-triazole-3-yl)thio)methyl)-4-ethyl-4H-1,2,4-triazole-3-thiol shows the same activity as Trimethoprim against P. aeruginosa (MIC is 62.5 µg/ml, MBC – 125 μg/ml), marked activity towards S. aureus (MIC is 15.6 μg/ml, MBC – 31.25 μg/ml while trimethoprim – 31.25 μg/ml, MBC – 62.5 µg/ml), greater fungistatic and fungicidal activity towards C. albicans, which amounted to 31.25 µg/ml, MFC – 62.5 µg/ml (while trimethoprim 62.5 µg/ml, MFC – 125 µg/ml). Reconstration of benzyldenhydrazide group to hydrazide one leads to the slight increase of bacteriostatic activity. Replacing the radical R from -Н to -C2H5 does not influence on the change of fungistatic activity. However, this resulted to the decrease in bacteriostatic activity against E. coli. The same is observed in relation to P. aeruginosa: from 62.5 μg/ml, MFC – 125 mcg/ml to 125 μg/ml, MFC – 125 µg/m respectively. Conclusion. The conducted study has showed that among the synthesized compounds there are some substances which strength of the antimicrobial and antifungal action in some cases exceeds the standard of comparison - Trimethoprim. The most active compound against the test strain of C. albicans was 2-(5-(((5-(adamantane-1-yl)-4H-1,2,4-triazole-3-yl)thio)methyl)-1,3,4-thiodiazole(todosol)-2-amine), which activity in 8 times higher than the standard of comparison. The study of biological and pharmacological activity of the synthesized compounds, and also the determination of the regularities between structure and activity create a favorable basis for the search of new medicines in a number of 1,2,4-triazole derivatives.

Implementation of new highly efficient drugs in medical practice is one of the most urgent problems of modern pharmaceutical industry. Scientific achievements of heterocyclic compounds’ chemistry clearly show that 1,2,4-triazole system is promising object of such research. It should be also noted that compounds, with adamantane "core" deserve special attention. The aim of our work was to synthesize new derivatives of 5-(adamantan-1-yl)-4-amino-1,2,4-triazole-3-thiols, and to investigate their physical and chemical properties. Materials and methods. Chemical names of compounds are given in accordance with IUPAC nomenclature (1979) and the IUPAC recommendations (1993). Study of physical-chemical properties of obtained compounds was carried out in accordance with methods listed in the State Pharmacopia of Ukraine. Melting point was determined on the automatic device for determining the melting temperature Stanford Research Systems OptiMelt MPA100 (manufacturing USA). Elemental composition of new compounds was established on the elemental analyzer Elementar Vario EL cube (CHNS) (standard – sulfanilamide). The data of elemental analysis correspond to the calculated one. 1H NMR spectra of compounds were recorded using spectrometer "Mercury 400". Chromato-mass spectra were recorded on a spectrometer Agilent 6890N/5973N/FID production of Agilent Technologies. Results. The concept of creating biologically active molecules is first of all directed on combining fragments of different molecules in one. Theoretically, this could lead to the new types of compounds with pharmacological action, and sometimes to the potentiation of existing biological properties. Further actions are focused on the physical and chemical properties study of these compounds. To establish the structure of 5-(adamantan-1-yl)-4-((aryl-, heteryl)ilyden)-amino-1,2,4-triazole-3-thiols we used an integrated approach with the use of modern physical and chemical methods of analysis. Individuality of compounds in each case is confirmed by chromatography. The results of the chromatographic investigations showed that in all cases the constants of pseudo molecular ions peaks of obtained 5-(adamantan-1-yl)-4-((aryl-, heteryl)yliden)amino-1,2,4-triazole-3-thiols coincide with theoretical calculations of molecular masses. Conclusions. New 5-(adamantan-1-yl)-4-((aryl-, heteryl)yliden)amino-1,2,4-triazole-3-thiols have been synthesized, their structure and individuality has been confirmed by complex of modern physical-chemical methods of analysis. In all cases, the values of the investigated physical-chemical constants coincide with the calculated ones.

Aim. The modern arsenal of antimicrobial drugs is characterized by high toxicity, moreover their widespread and uncontrolled use leads to the resistant strains’ selection. It creates favorable basis for the synthesis of new compounds with pronounced pharmacological activity. Methods and results. The primary screening of antimicrobial and antifungal activity of synthesized Nʹ-ylidene-2(3-(adamantan-1-yl)-1H-1,2,4-triazole-5-yl)thioacetohydrazides has been conducted by twofold serial dilutions method in liquid nutrient media in order to identify among the synthesized compounds those ones which have high antimicrobial and antifungal activities. Due to the results of the study the compounds that exceed the standard, according to the activity of comparison, have been identified. Some relationship’s patterns as «structure-activity» have been established. Conclusion. It indicates the need to expand the search spectrum of promising compounds towards the museum and clinical strains of infectious diseases’ pathogens.

Heterocyclic systems, in particular, 1,2,4-triazole derivatives cause great interest in the development of new medicinal substances for the production of highly effective and safe antihypoxants. This fact has created favorable basis for the synthesis of new alkylderivatives 5-(adamantane-1-yl)-4-R-1,2,4-triazole-3-thion, where R is methyl, phenyl. Aim. In order to identify the most promising active compounds the pharmacological screening of anti-hypoxemic activity of synthesized compounds has been carried out. Results. Due to the results of the study a number of compounds with anti-hypoxemic activity have been revealed. Some regularities between structure and pharmacological effect have been established. Conclusion. This demonstrates the prospects of further studies of the obtained compounds.

Aim. To study the acute toxicity of 2-(5-(adamantane-1-yl)-4R-1,2,4-triazole-3-ylthio) acetic acids and their salts’ derivatives. Methods and results. According to the data the acute toxicity of investigated substances belongs to the 4th class of toxicity and they are low-toxic, their LD50 ranges from 306 to 1520 mg/kg. The most toxic compound among the investigated compounds is derived thioacetic acid containing N4 nitrogen atom of the methyl substitute and its medium lethal dose (LD50) is 306 mg/kg. It is indicated that among the studied derived thioacetic acids and their salts’ derivatives the least toxic is also derived thioacetic acid containing instead of N4 nitrogen atom phenyl radical, and its LD50 is 1520 mg/kg. After analyzing the data obtained by experimental studies of acute toxicity and chemical structure of the investigated compounds some patterns concerning the chemical structure and acute toxicity have been established. Conclusion. In the row of derived thioacetic acids from № 9 to № 1 and № 3 their toxicity increased in this case the loss of phenyl substitute of the acid 9 accompanied by a insignificant increase of toxicity, where the substitution of this phenyl substitute on the methyl leads to a significant toxicity’s increase on 1214 mg/kg and is 306 mg/kg unlike 1520 mg/kg.

The concentration of glucose in the blood is one of the integral indicators of the internal environment that reflects the metabolism of carbohydrates, proteins and fats in the body. Glucose is a key component of human metabolism. The level of blood sugar is one of the most important controlled constants of body that defines homeostasis and displays the status of carbohydrate metabolism. Low blood sugar (hypoglycemia) is a dangerous condition when blood glucose is critically low. The aim of research. The aim of our study was pharmacological screening of the effects of the newly synthesized salts of 2-(5-adamantane-1-yl)-4-R-1,2,4-tirazol-3-yltio)acetic acids on glucose blood level in experimental animals. Materials and methods. The compounds have been synthesized at the Department of Inorganic Chemistry and Toxicology of Zaporozhye State Medical University (the Head of the chair, Professor Panasenko O.I.). Blood glucose levels of saults of2-(5-adamantane-1-yl)-4-R-1,2,4-tirazol-3-yltio)acetic acids in rats have been evaluated by intraperitoneal glucose tolerance test (IGTT). IGTT was reproduced by glucose load on animals in a dose of 2 g / kg of rat’s body weight. White nonlinear rats weighing 160-230 g were involved in experiments. Animals were divided into 13 groups of 7 animals in each group: the 1st is intact one; the 2nd is control one with glycemia (untreated), the 3rd group of the animals received glibenclamide in the dose of 1 mg / kg; the groups 3-13 received 1,2,4-triazole derivatives. The compounds were dissolved in purified water at the rate of 1 ml per 100 g of animals’ weight and were injected intraperitoneally. Glucose content in blood has been measured by glucosidase method using glucometer «Accu Chek Active» in 30 minutes from the load moment. The research results have been processed by modern statistical methods of analysis on a personal computer using a standard software package Microsoft Office 2010 (Microsoft Excel) and «STATISTICA® for Windows 6.0». Arithmetic means (M) and standard error of the average (± m) were calculated. The reliability of intergroup differences in experimental data was set by means of the Student's t-test. We used 3 levels of statistical significance of differences of research results - p