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In the current scenario, all the developing countries majorly depend on the Transportation sector for this they need a high volume of fuel resources this made the developing countries bargain from the oil-producing countries. Due to the high demand for fuel, price hike which brings a burden to the people, emission of CO2 leads to the pollution which in turn causing damage to the Atmosphere. To reduce the pollution and price hike we need an alternate solution like Biodiesel for this the biodiesel from palmarosa grass oil can be used as fuel at the different blends, the properties of the fuel, Performance, Emission, brake thermal efficiency, Specific fuel consumption are analysed at the different blends of Diesel and Biodiesel at the four-stroke diesel engine at different load condition and various mixtures of biofuel (P10, P20, P30). The result of the experiment shows that biodiesel blends P30 gives the maximum brake thermal efficiency of 35.18% which is comparatively lower than diesel. Thus, the biodiesel blend of P30 has a reduction in the emission of CO2 and HC at the level of 5% and 4%, where carbon monoxide emission is slightly reduced compared to diesel.