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ABSTRACT Habitat loss threatens biodiversity worldwide being particularly detrimental in tropical rainforests where a cumulative deforestation expands for decades. Tropical rainforests harbor a rich mammal diversity with a wide range of species using different habitats, ranging from forest‐dwelling to arboreal species. Recent techniques such as camera trapping have proven to be useful to study the ecology of arboreal mammals. Here, we assessed the overall community structure of arboreal mammals in a protected area by analyzing the patterns of diversity and abundance and their spatial and seasonal variations. A total of 21 camera‐trapping stations were set in clusters in three zones. Spatial and seasonal alpha‐diversity and community evenness patterns were estimated using Hill's numbers, and Sørensen's dissimilarities were used as a proxy to estimate beta‐diversity. A relative abundance index was calculated for each species, at each site and season. To estimate the influence of spatial and tree morphology on arboreal use by mammals, a Principal Component Analysis was performed. We observed a high species richness (14 species) of arboreal mammals. Species richness remained similar between sites, although shifts in abundances and a decreasing gradient in community evenness related to the distance of camera trap station located in each site were noted. We observed a high‐ and low‐diversity dissimilarity between camera trap stations and between zones, respectively. Seasonality showed no significant effect over abundance, alpha, and beta diversities. This protected area holds the natural habitat conditions to ensure the persistence of this rich arboreal mammal community.

Myeloid-derived suppressor cells (MDSCs) are immature cells with an immunosuppressive function. MDSCs have been related to inflammation in many settings, including infections, transplantation, obesity, aging, or cancer. In oncological settings, MDSCs participate in tumor immunoescape, growth, and metastasis. Certain nutrients can modify chronic inflammation by their interaction with MDSCs. Therefore, the possible influence of certain nutrients on immune surveillance by their actions on MDSCs and how this may affect the prognosis of cancer patients were evaluated in this scoping review. We identified seven papers, six of which were murine model studies and only one was a human clinical trial. Globally, a significant reduction in cancer growth and progression was observed after achieving a reduction in both MDSCs and their immunosuppressive ability with nutrients such as selected vegetables, icaritin, retinoic acid, curdlan, active vitamin D, soy isoflavones, and green tea. In conclusion, the consumption of certain nutrients may have effects on MDSCs, with beneficial results not only in the prevention of tumor development and growth but also in improving patients’ response.

The Break4Brain project aims to elucidate the effects of both acute and chronic physical activity (PA) on educational achievement in children with and without Attention Deficit Hyperactivity Disorder (ADHD). This study will be conducted in two phases: a cross-over design followed by a hybrid type 1 implementation-effectiveness trial, which includes both a cluster randomized controlled trial (RCT) and a qualitative study. In phase I, 60 children aged 10–12, with 30 each from ADHD and non-ADHD groups, will participate in a laboratory-based study over 4 days within 1 month. They will participate in three counterbalanced experimental conditions: (i) PA with cognitive engagement, (ii) PA without cognitive engagement, and (iii) a cognitively engaging control. This phase will assess acute changes in brain function, academic performance, working memory, inhibitory control, and sustained attention. Phase II will involve 600 children aged 10–12, randomly assigned to either a video-based PA program or a control group (300 children per group) in an 8-week cluster RCT. This phase will also incorporate a qualitative approach to explore the implementation context through pre- and post-intervention semi-structured interviews with teachers and school staff, and questionnaires for students. The outcomes of interest in this phase will include working memory, cognitive flexibility, selective attention, and academic performance. For the cross-over study, we hypothesize that PA conditions will enhance the studied outcomes compared to the control condition. In the RCT, we anticipate that the 8-week active breaks program will result in significant improvements in the selected outcomes compared to the control group. This study is expected to make pioneering contributions by including novel variables and focusing on the ADHD population. Furthermore, if the cluster RCT proves effective, it could offer a practical and cost-effective resource for integrating active breaks into daily school routines.

The Trypanosomatidae family encompasses around 24 genera of unicellular protozoans, many of which are transmitted by various hematophagous arthropods, particularly members of the Orders Diptera and Hemiptera. Fleas and ticks—an understudied group of ectoparasites—have been shown to be hosts of a wide and crescent variety of trypanosomatid species. Further, fleas and ticks of companion animals have been particularly neglected in trypanosomatid surveillance despite the proximity to human populations and the anthropophagous habits of many of these arthropods, which can potentially act as vectors of zoonotic trypanosomatids. We aimed to identify the presence, characterize the species, and establish the prevalence of Kinetoplastids, including members of the Trypanosomatidae family, in ectoparasites collected from dogs and humans from Mexico. A total of 537 ectoparasite specimens belonging to six ectoparasite taxa (Amblyomma mixtum, A. tenellum, Ctenocephalides felis felis, Pulex simulans, Rhipicephalus linnaei, and Rh. sanguineus s.s.) were collected from 15 States of Mexico. An 800 bp fragment of the 18S-rDNA gene from kinetoplastids was amplified and sequenced. The presence of two agents (Trypanosoma caninum and Parabodo sp.) was detected in R. linnaei ticks and one (Blechomonas lauriereadi) in the cat flea Ct. felis felis. This is the first record of genetic material of kinetoplastid species in ectoparasites from dogs and humans in Mexico.

The seabird intestinal microbiota, or bacteriome, predominantly consists of bacteria that establish during embryonic development through vertical transfer from females and horizontal transfer during growth and copulation. This study focuses on the Heermann’s Gull (Larus heermanni), a seabird that breeds mainly within the Gulf of California. Our goal was to understand its gut bacteriome composition and its implications for conservation and public health. Using microbiological techniques, 16S rRNA gene sequencing, and mass spectrometry, we characterized the HG gut bacteriome and compared it with other bird species. Our findings reveal a diverse bacteriome, particularly in the intestine and rectum, with dominant phyla including Firmicutes, Proteobacteria, Bacteroidetes, Cyanobacteria, and Fusobacteria. Seasonal and sex-related differences were observed, with significant correlations between some pairs of bacteria. Notably, we identified various pathogens and potential zoonotic bacteria, underscoring the public health risks associated with Heermann’s Gull interaction with humans and other animals. These results highlight the importance of the Heermann’s Gull gut bacteriome in nutrient production, pathogen control, and digestive health, and emphasize the need for further research on pathogen transmission involving seabirds. Our study provides crucial insights for conservation strategies and underscores the role of wild birds in the epidemiology of zoonotic diseases.

Abstract Anthropogenic loss of biodiversity continues to increase worldwide, and existing conservation area networks (CANs) are inadequate for its adequate representation and persistence. To identify a set of new nominal conservation areas in Oaxaca, a Mesoamerican biodiversity hotspot in Mexico, for terrestrial vertebrate species, we used a multi‐criteria systematic conservation planning approach. Besides minimizing the area incorporated into the nominal CAN, we incorporated 25 socioeconomic variables using multi‐attribute value theory. We constructed a portfolio of nominal CAN solutions for four different scenarios all of which satisfied a 10% representation target for the modeled suitable habitat of each vertebrate species: (1) existing protected area‐based (PA) solution; (2) voluntary conservation area‐based (VCA) solution; (3) PA‐VCA solution; and (4) R‐C solution (rarity‐complementary algorithm). The PA‐VCA and PA solutions were the most expensive in terms of area that had to be included in the nominal CANs (13,352 km2 and 12,587 km2, respectively). In all our multi‐criteria analyses, highest costs were associated with maximizing the number of airports, amount of tourism, and length of available highways in a nominal CAN. We have thus established a portfolio of multi‐criteria solutions to the problem of creating an adequate CAN for the representation of terrestrial vertebrate species.

BackgroundDiffuse large B cell lymphoma (DLBCL) is the most common non-Hodgkin lymphoma worldwide. DLBCL is an aggressive disease that can be cured with upfront standard chemoimmunotherapy schedules. However, in approximately 35-40% of the patients DLBCL relapses, and therefore, especially in this setting, the search for new prognostic and predictive biomarkers is an urgent need. Natural killer (NK) are effector cells characterized by playing an important role in antitumor immunity due to their cytotoxic capacity and a subset of circulating NK that express CD8 have a higher cytotoxic function. In this substudy of the R2-GDP-GOTEL trial, we have evaluated blood CD8+ NK cells as a predictor of treatment response and survival in relapsed/refractory (R/R) DLBCL patients.Methods78 patients received the R2-GDP schedule in the phase II trial. Blood samples were analyzed by flow cytometry. Statistical analyses were carried out in order to identify the prognostic potential of CD8+ NKs at baseline in R/R DLBCL patients.ResultsOur results showed that the number of circulating CD8+ NKs in R/R DLBCL patients were lower than in healthy donors, and it did not change during and after treatment. Nevertheless, the level of blood CD8+ NKs at baseline was associated with complete responses in patients with R/R DLBCL. In addition, we also demonstrated that CD8+ NKs levels have potential prognostic value in terms of overall survival in R/R DLBCL patients.ConclusionCD8+ NKs represent a new biomarker with prediction and prognosis potential to be considered in the clinical management of patients with R/R DLBCL.Clinical trial registrationhttps://www.clinicaltrialsregister.eu/ctr-search/search?query=2014-001620-29 EudraCT, ID:2014-001620-29.

Bats provide important ecosystem services for agriculture, such as pest control, a function that is particularly relevant for small-scale farmers. However, climate change is causing a decrease in bat populations. To assess the potential impacts of climate change on insectivorous bats and the implications on small-scale farming of indigenous communities in the Chiapas Highlands in southern Mexico we developed a three-step protocol: (1) projecting distribution shifts of insectivorous bats under climate change scenarios using non-dispersal and limited-full dispersal assumptions, (2) using official information to estimate the average economic value of conducting chemical pest control in crops at a state level, (3) surveying small-scale farmers to estimate the local economic value of pest control and determine how bats are perceived by small-scale farmers. Our models project shifts in bat species due to climate change. Given that new suitable climatic areas are also projected, if we assume a limited-full dispersal scenario, bats might not be as at risk by climate change, but shifting their distribution to more suitable habitats will probably affect the dynamic of the ecosystem service they provide. The official estimated value of chemical pest control is $15.15 USD/ha, while the estimated cost resulting from a hybrid survey with small-scale farmers was $47.53 USD/ha. The difference in cost could be related to an overuse of insecticides for pest control or an increase in price due to reduced accessibility. Sixty percent of surveyed farmers perceived a decline in bat populations, 68% were unaware of the benefits that bats provide to their crops, 51% believe that bats are mainly hematophagous, and 10% recognize that people harm or kill bats. A new approach including communicating small-scale farmers in their native languages the benefits that insectivorous bats provide along with a pest management strategy for the efficient use of insecticides needs to be implemented.

ALVARGONZÁLEZ FERNÁDEZ, Manuel, José María de Torrijos y Uriarte. Más allá del cuadro de Gisbert. Madrid, Sílex, 2021, 406 pp.

SARS-CoV-2 infection is the cause of the disease named COVID-19, a major public health challenge worldwide. Differences in the severity, complications and outcomes of the COVID-19 are intriguing and, patients with similar baseline clinical conditions may have very different evolution. Myeloid-derived suppressor cells (MDSCs) have been previously found to be recruited by the SARS-CoV-2 infection and may be a marker of clinical evolution in these patients. We have studied 90 consecutive patients admitted in the hospital before the vaccination program started in the general population, to measure MDSCs and lymphocyte subpopulations at admission and one week after to assess the possible association with unfavorable outcomes (dead or Intensive Care Unit admission). We analyzed MDSCs and lymphocyte subpopulations by flow cytometry. In the 72 patients discharged from the hospital, there were significant decreases in the monocytic and total MDSC populations measured in peripheral blood after one week but, most importantly, the number of MDSCs (total and both monocytic and granulocytic subsets) were much higher in the 18 patients with unfavorable outcome. In conclusion, the number of circulating MDSCs may be a good marker of evolution in the follow-up of unvaccinated patients admitted in the hospital with the diagnosis of COVID-19.

Gestational diabetes mellitus (GDM) is the most frequent pathophysiological state of pregnancy, which in many cases produces fetuses with macrosomia, requiring increased nutrient transport in the placenta. Recent studies by our group have demonstrated that leptin is a key hormone in placental physiology, and its expression is increased in placentas affected by GDM. However, the effect of leptin on placental nutrient transport, such as transport of glucose, amino acids, and lipids, is not fully understood. Thus, we aimed to review literature on the leptin effect involved in placental nutrient transport as well as activated leptin signaling pathways involved in the expression of placental transporters, which may contribute to an increase in placental nutrient transport in human pregnancies complicated by GDM. Leptin appears to be a relevant key hormone that regulates placental transport, and this regulation is altered in pathophysiological conditions such as gestational diabetes. Adaptations in the placental capacity to transport glucose, amino acids, and lipids may underlie both under- or overgrowth of the fetus when maternal nutrient and hormone levels are altered due to changes in maternal nutrition or metabolic disease. Implementing new strategies to modulate placental transport may improve maternal health and prove effective in normalizing fetal growth in cases of intrauterine growth restriction and fetal overgrowth. However, further studies are needed to confirm this hypothesis.

Type 2 diabetes, insulin resistance, and obesity are strongly associated and are a major health problem worldwide. Obesity largely results from a sustained imbalance between energy intake and expenditure. Therapeutic approaches targeting metabolic rate may counteract body weight gain and insulin resistance. Bone morphogenic protein 7 (BMP7) has proven to enhance energy expenditure by inducing non-shivering thermogenesis in short-term studies in mice treated with the recombinant protein or adenoviral vectors encoding BMP7. To achieve long-term BMP7 effects, the use of adeno-associated viral (AAV) vectors would provide sustained production of the protein after a single administration. Here, we demonstrated that treatment of high-fat-diet-fed mice and ob/ob mice with liver-directed AAV-BMP7 vectors enabled a long-lasting increase in circulating levels of this factor. This rise in BMP7 concentration induced browning of white adipose tissue (WAT) and activation of brown adipose tissue, which enhanced energy expenditure, and reversed WAT hypertrophy, hepatic steatosis, and WAT and liver inflammation, ultimately resulting in normalization of body weight and insulin resistance. This study highlights the potential of AAV-BMP7-mediated gene therapy for the treatment of insulin resistance, type 2 diabetes, and obesity.

Abstract Range verification of clinical protontherapy systems via positron-emission tomography (PET) is not a mature technology, suffering from two major issues: insufficient signal from low-energy protons in the Bragg peak area and biological washout of PET emitters. The use of contrast agents including 18O, 68Zn or 63Cu, isotopes with a high cross section for low-energy protons in nuclear reactions producing PET emitters, has been proposed to enhance the PET signal in the last millimeters of the proton path. However, it remains a challenge to achieve sufficient concentrations of these isotopes in the target volume. Here we investigate the possibilities of 18O-enriched water (18-W), a potential contrast agent that could be incorporated in large proportions in live tissues by replacing regular water. We hypothesize that 18-W could also mitigate the problem of biological washout, as PET (18F) isotopes created inside live cells would remain trapped in the form of fluoride anions (F-), allowing its signal to be detected even hours after irradiation. To test our hypothesis, we designed an experiment with two main goals: first, prove that 18-W can incorporate enough 18O into a living organism to produce a detectable signal from 18F after proton irradiation, and second, determine the amount of activity that remains trapped inside the cells. The experiment was performed on a chicken embryo chorioallantoic membrane tumor model of head and neck cancer. Seven eggs with visible tumors were infused with 18-W and irradiated with 8-MeV protons (range in water: 0.74 mm), equivalent to clinical protons at the end of particle range. The activity produced after irradiation was detected and quantified in a small-animal PET-CT scanner, and further studied by placing ex-vivo tumours in a gamma radiation detector. In the acquired images, specific activity of 18F (originating from 18-W) could be detected in the tumour area of the alive chicken embryo up to 9 h after irradiation, which confirms that low-energy protons can indeed produce a detectable PET signal if a suitable contrast agent is employed. Moreover, dynamic PET studies in two of the eggs evidenced a minimal effect of biological washout, with 68% retained specific 18F activity at 8 h after irradiation. Furthermore, ex-vivo analysis of 4 irradiated tumours showed that up to 3% of oxygen atoms in the targets were replaced by 18O from infused 18-W, and evidenced an entrapment of 59% for specific activity of 18F after washing, supporting our hypothesis that F- ions remain trapped within the cells. An infusion of 18-W can incorporate 18O in animal tissues by replacing regular water inside cells, producing a PET signal when irradiated with low-energy protons that could be used for range verification in protontherapy. 18F produced inside cells remains entrapped and suffers from minimal biological washout, allowing for a sharper localization with longer PET acquisitions. Further studies must evaluate the feasibility of this technique in dosimetric conditions closer to clinical practice, in order to define potential protocols for its use in patients.

Esta aportación refleja los resultados de la investigación realizada durante el curso 2018/19 en relación con la introducción juegos de rol en la didáctica de la Historia Antigua en Educación superior. Ante la problemática detectada en diferentes titulaciones con respecto al nuevo alumnado y adaptándonos a las exigencias del aprendizaje competencial, se ha diseñado como alternativa metodológica un juego de rol de contenido histórico, Iberia. Este juego se ha aplicado en diferentes grupos y se ha evaluado su impacto en el alumnado como recurso didáctico a la hora de enseñar Historia Antigua en general y de la península ibérica en particular. Durante el curso 2018/19 ha realizado una prueba piloto del juego diseñado con nueve grupos de entre 30 y 60 alumnos y se han tomado tres de ellos como muestra, siendo encuestados sobre la actividad por medio de un cuestionario elaborado por el equipo docente. Los resultados obtenidos de la prueba piloto por medio de la observación directa y la elaboración y uso de un cuestionario plantean una clara aceptación del recurso para las clases prácticas, acercando al alumno a temas concretos como son los procesos económicos y sociales propios de la Historia Antigua de la península ibérica, y ha provocado una mejoría en la comprensión de estos por parte del alumnado.

Maize (Zea maysL.) germplasm from the Ecuadorian Higlands has low tolerance to inbreeding especially in starchy cultivars. To create competitive varieties at the commercial level it is necessary to identify outstanding progenitor lines by early testing. The purpose of this research was to evaluate the yield potential of S1linesof black corn by early testing. The experiment was conducted at the “Tunshi” Experiment Station, Licto parish, Chimborazo province, Ecuador using a complete random block design with four replications. Twenty-four treatments were evaluated, each consisting of an S1line of black corn. Agronomic traits were registered and the percentage of inbreeding depression was calculated. The lines presented statistical differences (P ≤ 0.05) in the variables days to male and female flowering, plant height, ear height, leaf disease, ear length, weight of 100 kernels and grain yield. All the variables showed inbreeding depression, except days to male flowering, which presented a low increase. Lines 16, 15 and 22 were the most productive with yields of 2.00; 2.08 and 1.95 t ha-1respectively. These resultsallow to select lines with less depressive effects on yield produced by inbreeding, and to continue with the improvement processes of black maize.

Ecosystems are responsible for strong feedback processes that affect climate. The mechanisms and consequences of this feedback are uncertain and must be studied to evaluate their influence on global climate change. The main objective of this study is to assess the gross primary production (GPP) dynamics and the energy partitioning patterns in three different European forest ecosystems through time series analysis. The forest types are an Evergreen Needleleaf Forest in Finland (ENF_FI), a Deciduous Broadleaf Forest in Denmark (DBF_DK), and a Mediterranean Savanna Forest in Spain (SAV_SP). Buys-Ballot tables were used to study the intra-annual variability of meteorological data, energy fluxes, and GPP, whereas the autocorrelation function was used to assess the inter-annual dynamics. Finally, the causality of GPP and energy fluxes was studied with Granger causality tests. The autocorrelation function of the GPP, meteorological variables, and energy fluxes revealed that the Mediterranean ecosystem is more irregular and shows lower memory in the long term than in the short term. On the other hand, the Granger causality tests showed that the vegetation feedback to the atmosphere was more noticeable in the ENF_FI and the DBF_DK in the short term, influencing latent and sensible heat fluxes. In conclusion, the impact of the vegetation on the atmosphere influences the energy partitioning in a different way depending on the vegetation type, which makes the study of the vegetation dynamics essential at the local scale to parameterize these processes with more detail and build improved global models.

La crisis de los cuidados en España motivó cambios importantes en la provisión de los cuidados, con cambios normativos que han regulado el derecho a los cuidados y las condiciones de un sector altamente feminizado. Estas transformaciones han acontecido con crisis globales que han tenido un intenso impacto en España, como la del 2008 o la pandemia de la COVID-19. Este trabajo analiza el impacto que estas coyunturas, y especialmente esta pandemia, han tenido en las condiciones de vida de las mujeres migrantes trabajadoras en el sector doméstico y de los cuidados. Sus testimonios recogen el impacto de las medidas implementadas y las consecuencias de ello en sus condiciones laborales y de vida. Los resultados cristalizan un sector en continua crisis, que requiere desarrollar estrategias de supervivencia y cuyas consecuencias tienen fuertes costes para las trayectorias de vida de estas trabajadoras esenciales.

Delivery of adeno-associated viral vectors (AAVs) to cerebrospinal fluid (CSF) has emerged as a promising approach to achieve widespread transduction of the central nervous system (CNS) and peripheral nervous system (PNS), with direct applicability to the treatment of a wide range of neurological diseases, particularly lysosomal storage diseases. Although studies in small animal models have provided proof of concept and experiments in large animals demonstrated feasibility in bigger brains, there is not much information on long-term safety or durability of the effect. Here, we report a 7-year study in healthy beagle dogs after intra-CSF delivery of a single, clinically relevant dose (2 × 1013 vg/dog) of AAV9 vectors carrying the canine sulfamidase, the enzyme deficient in mucopolysaccharidosis type IIIA. Periodic monitoring of CSF and blood, clinical and neurological evaluations, and magnetic resonance and ultrasound imaging of target organs demonstrated no toxicity related to treatment. AAV9-mediated gene transfer resulted in detection of sulfamidase activity in CSF throughout the study. Analysis at tissue level showed widespread sulfamidase expression and activity in the absence of histological findings in any region of encephalon, spinal cord, or dorsal root ganglia. Altogether, these results provide proof of durability of expression and long-term safety for intra-CSF delivery of AAV-based gene transfer vectors encoding therapeutic proteins to the CNS.

Summary: Background: Artificial intelligence (AI) is rapidly fuelling a fundamental transformation in the practice of pathology. However, clinical integration remains challenging, with no AI algorithms to date in routine adoption within typical anatomic pathology (AP) laboratories. This survey gathered current expert perspectives and expectations regarding the role of AI in AP from those with first-hand computational pathology and AI experience. Methods: Perspectives were solicited using the Delphi method from 24 subject matter experts between December 2020 and February 2021 regarding the anticipated role of AI in pathology by the year 2030. The study consisted of three consecutive rounds: 1) an open-ended, free response questionnaire generating a list of survey items; 2) a Likert-scale survey scored by experts and analysed for consensus; and 3) a repeat survey of items not reaching consensus to obtain further expert consensus. Findings: Consensus opinions were reached on 141 of 180 survey items (78.3%). Experts agreed that AI would be routinely and impactfully used within AP laboratory and pathologist clinical workflows by 2030. High consensus was reached on 100 items across nine categories encompassing the impact of AI on (1) pathology key performance indicators (KPIs) and (2) the pathology workforce and specific tasks performed by (3) pathologists and (4) AP lab technicians, as well as (5) specific AI applications and their likelihood of routine use by 2030, (6) AI's role in integrated diagnostics, (7) pathology tasks likely to be fully automated using AI, and (8) regulatory/legal and (9) ethical aspects of AI integration in pathology. Interpretation: This systematic consensus study details the expected short-to-mid-term impact of AI on pathology practice. These findings provide timely and relevant information regarding future care delivery in pathology and raise key practical, ethical, and legal challenges that must be addressed prior to AI's successful clinical implementation. Funding: No specific funding was provided for this study.

Phlebotomine sand flies (Diptera: Psychodidae: Phlebotominae) have biological relevance as vectors of several pathogens. To ensure periodic entomological monitoring it is necessary to have efficient and accurate tools for an adequate taxonomic identification. There are only few studies on phylogenetic analyses of phlebotomine sand flies from Neotropics, based mostly on morphological and/or molecular data, which makes the delimitation of intra- and interspecific variability of species challenging. Here we generated new molecular information on sand fly species distributed in endemic areas of leishmaniasis in Mexico, using mitochondrial and ribosomal genes, and incorporating morphological information available. Specifically, we established their phylogenetic relationships, and estimated their divergence time. Our study provides molecular information for 15 phlebotomine sand fly species from different areas of Mexico, contributing to the genetic inventory and phylogenetic relations among Neotropical species of the subfamily Phlebotominae. Mitochondrial genes proved to be suitable markers for the molecular identification of phlebotomine sand flies. However, the incorporation of additional nuclear gene information could increase the significance of phylogenetic inferences. We also provided evidence about a possible divergence time of phlebotomine sand fly species, supporting their presumable origin in the Cretaceous period.

Unidentified abortion, of which leptospirosis, brucellosis, and ovine enzootic abortion are important factors, is the main cause of disease spread between animals and humans in all agricultural systems in most developing countries. Although there are well-defined risk factors for these diseases, these characteristics do not represent the prevalence of the disease in different regions. This study predicts the unidentified abortion burden from multi-microorganisms in ewes based on an artificial neural networks approach and the GLM. Methods: A two-stage cluster survey design was conducted to estimate the seroprevalence of abortifacient microorganisms and to identify putative factors of infectious abortion. Results: The overall seroprevalence of Brucella was 70.7%, while Leptospira spp. was 55.2%, C. abortus was 21.9%, and B. ovis was 7.4%. Serological detection with four abortion-causing microorganisms was determined only in 0.87% of sheep sampled. The best GLM is integrated via serological detection of serovar Hardjo and Brucella ovis in animals of the slopes with elevation between 2600 and 2800 meters above sea level from the municipality of Xalatlaco. Other covariates included in the GLM, such as the sheep pen built with materials of metal grids and untreated wood, dirt and concrete floors, bed of straw, and the well water supply were also remained independently associated with infectious abortion. Approximately 80% of those respondents did not wear gloves or masks to prevent the transmission of the abortifacient zoonotic microorganisms. Conclusions: Sensitizing stakeholders on good agricultural practices could improve public health surveillance. Further studies on the effect of animal–human transmission in such a setting is worthwhile to further support the One Health initiative.

Proton therapy allows the treatment of specific areas and avoids the surrounding tissues. However, this technique has uncertainties in terms of the distal dose fall-off. A promising approach to studying the proton range is the use of nanoparticles as proton-activatable agents that produce detectable signals. For this, we developed an iron oxide nanoparticle doped with Zn (IONP@Zn-cit) with a hydrodynamic size of 10 nm and stability in serum. Cytotoxicity, defined as half of the surveillance, was 100 μg Zn/mL in the U251 cell line. The effect on clonogenic cell death was tested after X-ray irradiation, which suggested a radioprotective effect of these nanoparticles at low concentrations (1–10 μg Zn/mL). To evaluate the production of positron emitters and prompt-gamma signals, IONP@Zn-cit was irradiated with protons, obtaining prompt-gamma signals at the lowest measured concentration (10 mg Zn/mL). Finally, 67Ga-IONP@Zn-cit showed accumulation in the liver and spleen and an accumulation in the tumor tissue of 0.95% ID/g in a mouse model of U251 cells. These results suggest the possibility of using Zn nanoparticles as proton-activatable agents to verify the range by prompt gamma detection and face the challenges of prompt gamma detection in a specific biological situation, opening different avenues to go forward in this field.

Mucopolysaccharidosis type IVA (MPSIVA) is a lysosomal storage disorder causing severe skeletal and non-skeletal alterations in patients. Here, the authors generate a MPSIVA rat model that mimics the disabling human pathology and develop an AAV9-Galns gene therapy to treat the disease.

It is widely accepted that the immune system includes molecular and cellular components that play a role in regulating and suppressing the effector immune response in almost any process in which the immune system is involved. Myeloid-derived suppressor cells (MDSCs) are described as a heterogeneous population of myeloid origin, immature state, with a strong capacity to suppress T cells and other immune populations. Although the initial characterization of these cells was strongly associated with pathological conditions such as cancer and then with chronic and acute infections, extensive evidence supports that MDSCs are also involved in physiological/non-pathological settings, including pregnancy, neonatal period, aging, and vaccination. Vaccination is one of the greatest public health achievements and has reduced mortality and morbidity caused by many pathogens. The primary goal of prophylactic vaccination is to induce protection against a potential pathogen by mimicking, at least in a part, the events that take place during its natural interaction with the host. This strategy allows the immune system to prepare humoral and cellular effector components to cope with the real infection. This approach has been successful in developing vaccines against many pathogens. However, when the infectious agents can evade and subvert the host immune system, inducing cells with regulatory/suppressive capacity, the development of vaccines may not be straightforward. Notably, there is a long list of complex pathogens that can expand MDSCs, for which a vaccine is still not available. Moreover, vaccination against numerous bacteria, viruses, parasites, and fungi has also been shown to cause MDSC expansion. Increases are not due to a particular adjuvant or immunization route; indeed, numerous adjuvants and immunization routes have been reported to cause an accumulation of this immunosuppressive population. Most of the reports describe that, according to their suppressive nature, MDSCs may limit vaccine efficacy. Taking into account the accumulated evidence supporting the involvement of MDSCs in vaccination, this review aims to compile the studies that highlight the role of MDSCs during the assessment of vaccines against pathogens.

Abstract Identification of the different elements intervening at the tumor microenvironment seems key to explain clinical evolution in several tumor types. In this study, a set of immune biomarkers (myeloid derived suppressor cells, regulatory T cells, and OX40 + and PD-1 + T lymphocytes counts) in peripheral blood of patients diagnosed with advanced breast cancer were analyzed along of first line antineoplastic therapy. Subsequently, a comparison between groups with clinical benefit versus progression of disease and with a healthy women cohort was executed. Results reflected that patients showed higher basal levels of myeloid derived suppressor cells (35.43, IR = 180.73 vs 17.53, IR = 16.96 cells/μl; p = 0.001) and regulatory T cells (32.05, IR = 29.84 vs 22.61, IR = 13.57 cells/μl; p = 0.001) in comparison with healthy women. Furthermore, an increase in the number of activated T lymphocytes (expressing OX40), a decrease of immune inhibitory cells (MDSCs and Tregs) and inhibited T lymphocytes (expressing PD-1) were observed along the treatment in patients with clinical benefit (p ≤ 0.001). The opposite trend was observed in the case of disease progression. These findings suggest that some critical immune elements can be easily detected and measured in peripheral blood, which open a new opportunity for translational research, as they seem to be correlated with clinical evolution, at least in ABC.

Abstract A new coronavirus respiratory disease (COVID-19) caused by the SARS-CoV-2 virus, surprised the entire world, producing social, economic, and health problems. The COVID-19 triggers a lung infection with a multiple proinflammatory cytokine storm in severe patients. Without effective and safe treatments, COVID-19 has killed thousands of people, becoming a pandemic. Stem cells have been suggested as a therapy for lung-related diseases. In particular, mesenchymal stem cells (MSCs) have been successfully tested in some clinical trials in patients with COVID-19. The encouraging results positioned MSCs as a possible cell therapy for COVID-19. The amniotic membrane from the human placenta at term is a valuable stem cell source, including human amniotic epithelial cells (hAECs) and human mesenchymal stromal cells (hAMSCs). Interestingly, amnion cells have immunoregulatory, regenerative, and anti-inflammatory properties. Moreover, hAECs and hAMSCs have been used both in preclinical studies and in clinical trials against respiratory diseases. They have reduced the inflammatory response and restored the pulmonary tissue architecture in lung injury in vivo models. Here, we review the existing data about the stem cells use for COVID-19 treatment, including the ongoing clinical trials. We also consider the non-cellular therapies that are being applied. Finally, we discuss the human amniotic membrane cells use in patients who suffer from immune/inflammatory lung diseases and hypothesize their possible use as a successful treatment against COVID-19.

Introduction: Polycystic ovary syndrome (PCOS) is a complex metabolic disorder associated with ovulatory dysfunction, hyperandrogenism, obesity, and insulin resistance, that leads to subfertility. Sam68 is an RNA-binding protein with signaling functions that is ubiquitously expressed, including gonads. Sam68 is recruited to leptin signaling, mediating different leptin actions. Objective: We aimed to investigate the role of Sam68 in leptin signaling, mediating the effect on aromatase expression in granulosa cells and the posibl e implication of Sam68 in the leptin resistance in PCOS. Materials and methods: Granulosa cells were from healthy donors (n = 25) and women with PCOS (n = 25), within the age range of 20 to 40 years, from Valencian Infertility Institute (IVI), Seville, Spain. Sam68 expression was inhibited by siRNA method and overexpressed by expression vector. Expression level was analysed by qPCR and immunoblot. Statistical significance was assessed by ANOVA follo wed by different post-hoc tests. A P value of

El artículo expone el análisis de las trayectorias ocupacionales de los sastres en los cantones de Esparza y Puntarenas durante el periodo 1950-1972, basado en una metodología de historias de vida. El trabajo distingue y problematiza las dinámicas de comportamiento de este grupo ocupacional en el mundo del trabajo de las economías en los cantones mencionados, alrededor de su inserción al mundo laboral. Con base en fuente primaria, específicamente las entrevistas realizadas a sastres de estos cantones durante el año 2016, reconstruye con información de primera mano, las dinámicas y trayectorias de estos artesanos durante la segunda mitad del siglo XX en las periferias costarricenses. La finalidad del trabajo subyace en un esfuerzo por integrar el estudio de estos grupos de artesanos perifericos, a una visión más amplia sobre otros estudios realizados sobre artesanos en el centro del país, desde un análisis con perspectiva comparada de casos.

En los últimos años es frecuente la impartición de la educación física (EF) en lengua inglesa, lo que puede influir sobre las percepciones que los alumnos[1] tienen sobre dicha asignatura. En el presente estudio se analizó el efecto de una metodología bilingüe, el code-switching, sobre la percepción de la comunicación docente y la motivación. La muestra estuvo compuesta por 94 estudiantes de entre 12 y 14 años (M= 13.76; DT=.86). El grupo experimental (n=46) estuvo formado por dos grupos pertenecientes a formas diferentes de implantación del bilingüismo (programa y sección) y el grupo control (n= 47) por dos grupos equivalentes a los anteriores. Previo a la intervención, se realizaron entrevistas a profesorado de EF; los cuestionarios pre y post fueron administrados al alumnado al inicio y al fin de la misma. Tras la intervención se hallaron diferencias en la motivación intrínseca de uno de los grupos experimentales. Los resultados sugieren que la percepción de la comunicación docente por parte de los alumnos no difiere en función de que la clase se imparta íntegramente en inglés o utilizando el code-switching. Se discuten las implicaciones educativas de estos hallazgos. Abstract. Over the last few years, the teaching of PE in English language has become frequent which could influence the perceptions that students have about this subject. In the present study the effect of a bilingual methodology, code-switching, on perceptions of teaching communication and motivation was analyzed. The sample was composed by 94 students aging from 12 to 14 (M=13,76; SD= .86). Experimental group (n=46) was formed by two classes with different bilingual programs (programa and sección) and control group (n=47), by other two equivalent groups. After the intervention significant differences in intrinsic motivation were found in one of the experimental groups. The findings suggest that the perceptions of teaching communication do not differ between students taught completely in English and students taught through code-switching. Educational implications of these findings are discussed.

Infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes a disease (coronavirus disease 2019, COVID-19) that may develop into a systemic disease with immunosuppression and death in its severe form. Myeloid-derived suppressive cells (MDSCs) are inhibitory cells that contribute to immunosuppression in patients with cancer and infection. Increased levels of MDSCs have been found in COVID-19 patients, although their role in the pathogenesis of severe COVID-19 has not been clarified. For this reason, we raised the question whether MDSCs could be useful in the follow-up of patients with severe COVID-19 in the intensive care unit (ICU). Thus, we monitored the immunological cells, including MDSCs, in 80 patients admitted into the ICU. After 1, 2, and 3 weeks, we examined for a possible association with mortality (40 patients). Although the basal levels of circulating MDSCs did not discriminate between the two groups of patients, the last measurement before the endpoint (death or ICU discharge) showed that patients discharged alive from the ICU had lower levels of granulocytic MDSCs (G-MDSCs), higher levels of activated lymphocytes, and lower levels of exhausted lymphocytes compared with patients who had a bad evolution (death). In conclusion, a steady increase of G-MDSCs during the follow-up of patients with severe COVID-19 was found in those who eventually died.

Photodynamic therapy is a treatment modality that can be used to treat various types of lesions. To produce cell death, reaching a certain threshold dose of reactive oxygen species (ROS) is required. The estimation of ROS production is of paramount importance to predict the depth of necrosis and to ensure that the volume to be treated receives doses higher than the threshold. In this study, we compared a theoretical model for PDT based on Monte Carlo simulations of light irradiance and rate equations with a rat liver model. At the end of the simulation, necrosis depths and volumes were estimated, as well as the photosensitizer (PS), oxygen, and ROS concentrations at each position of the treated area. From the in vivo study, we obtained the ROS concentration threshold of about 1 mM for Photogem in rat liver. This proposed method can be used for any PS or tissue, including tissues with multiple layers. The proposed method can be used to estimate parameters for any PS or tissue, including layered tissues, as long as their parameters are known. In addition, other protocols can be tested, or compared with the standard ones, providing the bases for analyzing a diverse range of photodynamic treatment scenarios.

In the present work, new CaO-based adsorbents were obtained by a fast solution combustion method and high-energy ball-milling process to study their CO2 capture behavior under different moderate pressure and temperature conditions. The as-prepared CaO products were characterized systematically using different analytical techniques such as X-ray diffraction, scanning electron microscopy and N2 physisorption measurements. The results showed that the CaO prepared by solution combustion and ball-milled during 2.5 h showed the maximum CO2 adsorption capacity of 9.31 mmol/g at 25 °C and 1 atm mainly via chemisorption with CaCO3 formation, which was corroborated by infrared spectroscopy, X-ray diffraction, and X-ray photoelectron spectroscopy studies. In general, the obtained results revealed that the synthesized CaO nanopowders from solution combustion that were treated by high-energy ball-milling enhanced their CO2 adsorption capacity due to improved structural and textural properties, and this CaO-based adsorbent can be used as a promising material for CO2 capture in post-combustion CO2 capture technologies on a large scale, under atmospheric pressure and temperature conditions. Keywords: CaO-based adsorbents, CO2 capture, Solution combustion synthesis, Ball-milling process, Textural properties, Structural influences

Esta aportación presenta los resultados de los proyectos de Innovación docente gestionados por miembros del Dpto. de H.ª Antigua en 2011 y 2017 para generar nuevas dinámicas de aprendizaje en nuestras materias y cambiar los materiales que utilizamos en las mismas a fin de modernizarlos y adecuarlos a las nuevas necesidades del alumnado. El primero de los proyectos realizó una labor de investigación para identificar las necesidades a nivel de contenidos del alumnado de la asignatura de Fundamentos de Historia en el grado de Educación Primaria, mientras que el segundo se centró en crear un nuevo modelo de manual universitario adecuado a las necesidades tanto de este alumnado como el de otros grados en los que el Dpto. imparte clases. Los objetivos que nos marcamos fueron identificar las características del alumnado y su grado de conocimientos, valorar el proceso de aprendizaje, identificar las principales dificultades que tienen frente a los contenidos de nuestras materias, diseñar unos nuevos materiales que las solucionaran y valorar el grado de éxito de los mismos. Para ello diseñamos un cuestionario que se pasó a todos los grupos que cursan la asignatura, teniendo la suerte de tener una muestra significativa de entre quinientos a seiscientos alumnos y durante varios años procuramos diseñar los nuevos materiales que vieron la luz en forma de libro electrónico maquetado y publicado gracias al proyecto de Innovación docente de 2016/17/ y que comenzó a usarse en el cuso 2016/17, presentando un aumento en el índice de aprobados y permitiéndonos reflexionar sobre la necesidad de continuar el proceso de reforma de contenidos, materiales y metodologías para estas asignaturas.

Background and objective: Polycystic ovary syndrome (PCOS) is a complex metabolic disorder associated with ovulatory dysfunction, hyperandrogenism, obesity, and insulin resistance, which leads to subfertility. PCOS is the most frequent metabolic disorder in women and the major cause of infertility. Susceptibility to developing PCOS is determined by a complex interaction between environmental and genetic factors. Although different mechanisms have been proposed to explain PCOS manifestations, defects in insulin actions or in the insulin signaling pathways are central in the pathogenesis of the syndrome. However, the mechanisms (molecular players and signaling pathways) underlying its primary origin still remain an unsolved issue. Current research is increasingly focusing on the discovery of novel biomarkers to further elucidate the complex pathophysiology of PCOS. Sam68, an RNA-binding protein, is recruited to insulin signaling, mediating different insulin actions. We aimed to investigate the role of Sam68 in insulin signaling and the possible implications of Sam68 in the insulin resistance in PCOS. Materials and methods: Granulosa cells were taken from women with PCOS (n = 25) and healthy donors (n = 25) and, within the age range of 20 to 42 years, from GINEMED, Assisted Reproduction Centre, Seville, Spain. The Sam68 expression level was analyzed both by qPCR and immunoblot. Statistical significance was assessed by one-way ANOVA, followed by a post-hoc test. A p value of < 0.05 was considered statistically significant. Results: We found that insulin stimulation increases the phosphorylation and expression level of Sam68 in granulosa cells from normal donors. The downregulation of Sam68 expression resulted in a lower activation of both the MAPK and the PI3K pathways in response to insulin. Moreover, the granulosa cells from the women with PCOS presented a lower expression of Sam68, as well as insulin receptor and insulin receptor substrate-1 (IRS-1). In these cells, the overexpression of Sam68 resulted in an increased activation of both the MAPK and the PI3K pathways in response to insulin. Conclusions: These results suggest the participation of Sam68 in insulin receptor signaling, mediating the insulin effect in granulosa cells, and they suggest the possible role of Sam68 in the insulin resistance of PCOS.

Asio flammeus es una especie de ave de la familia Strigidae, que habita en las zonas agrícolas, urbanas, humedales y lomas de la costa peruana, sin embargo, no había sido registrada en el Área de Conservación Privada Lomas del Cerro Campana, región La Libertad. Los registros fueron hechos entre los años 2016 y 2019, mediante observación directa. En todos los registros se observó un individuo de la especie, cuatro de los cuales fueron realizados en la base y uno en el piso inferior de este lugar. Con el reporte de Asio flammeus se incrementa a 39 la lista de especies de aves que ocurren en esta área. Palabras claves: Aves, Strigidae, lomas, Área de Conservación Privada Lomas del Cerro Campana ABSTRACT Asio flammeus is a bird of the Strigidae family. Which live in the agricultural, urban, wetlands and hills of the Peruvian coast. However, had not been registered in the Private Conservation Area Lomas del Cerro Campana, in La Libertad region. The records were made between the years 2016 and 2019, through direct observation. In all records, one individual of the species was observed, four of which were made at the base and one on the lower floor of this place. With the report of Asio flammeus the bird list species that are present in this area increases to 39. Keywords: Birds, Strigidae, hills, Lomas del Cerro Campana Private Conservation Area DOI: http://dx.doi.org/10.17268/rebiol.2020.40.02.16

Background Preventing infantile anaemia and ensuring optimal growth and development during early childhood, particularly in resource-constrained settings, represent an ongoing public health challenge. Current responses are aligned to treatment-based solutions, instead of determining the roles of its inter-related causes. This project aims to assess and understand the complex interplay of eco-bio-social-political factors that determine infantile anaemia to inform policy, research design and prevention practices.Methods This is a longitudinal birth cohort study including four components: (1) biological, will assess known blood markers of iron homeostasis and anaemia and stool microbiota to identify and genetically analyse the participants’ flora; (2) ecological, will assess and map pollutants in air, water and soil and evaluate features of nutrition and perceived food security; (3) social, which will use different qualitative research methodologies to explore key stakeholders and informants’ perceptions related to nutritional, environmental and anaemia topics, participant observations and a participatory approach and (4) a political analysis, to identify and assess the impact of policies, guidelines and programmes at all levels for infantile anaemia in the three regions. Finally, we will also explore the role of social determinants and demographic variables longitudinally for all study participants. This project aims to contribute to the evidence of the inter-related causal factors of infantile anaemia, addressing the complexity of influencing factors from diverse methodological angles. We will assess infantile anaemia in three regions of Peru, including newborns and their mothers as participants, from childbirth until their first year of age.Ethics and dissemination Ethical approval was obtained from the Institutional Research Ethics Committee of the Instituto Nacional de Salud del Niño (Lima, Peru), CIEI-043-2019. An additional opinion has been granted by the Ethical Committee of Queen Mary University of London (London, UK). Dissemination across stakeholders is taking part as a continues part of the research process.

The length of the day (photoperiod) is a robust seasonal signal originated by earth orbital and translational movements, a resilient external cue to the global climate change, and a predictable hint to initiate or complete different developmental programs. In eukaryotic algae, the gene expression network that controls the cellular response to photoperiod also regulates other basic physiological functions such as starch synthesis or redox homeostasis. Land plants, evolving in a novel and demanding environment, imbued these external signals within the regulatory networks controlling organogenesis and developmental programs. Unlike algae that largely have to deal with cellular physical cues, within the course of evolution land plants had to transfer this external information from the receiving organs to the target tissues, and mobile signals such as hormones were recruited and incorporated in the regulomes. Control of senescence by photoperiod, as suggested in this perspective, would be an accurate way to feed seasonal information into a newly developed function (senescence) using an ancient route (photoperiodic signaling). This way, the plant would assure that two coordinated aspects of development such as flowering and organ senescence were sequentially controlled. As in the case of senescence, there is growing evidence to support the idea that harnessing the reliability of photoperiod regulation over other, more labile signaling pathways could be used as a robust breeding tool to enhance plants against the harmful effects of climate change.

El objetivo de este trabajo fue evaluar el impacto de una intervención escolar en control inhibitorio llevada a cabo dentro de diversas aulas de segundo ciclo de primaria (9-11 años) que tenían alumnos con Trastorno del Desarrollo del Lenguaje (n = 5) o dislexia (n = 4) y sus compañeros de aula sin dificultades (n = 6). El diseño siguió un modelo pre-post intervención con las variables dependientes fluidez verbal, memoria fonológica y control inhibitorio. La intervención consistió en ocho sesiones de 10 minutos a todo el grupo clase, dos por semana durante cuatro semanas, más una sesión larga de 1 hora en grupo pequeño en medio de las sesiones, en las que se realizaba actividades basadas en diferentes tareas de control inhibitorio (“Stroop”, “Go-no go”, “Plots”, “Simon”,…). Los niños con TDL y dislexia mostraron una puntuación más baja en memoria fonológica antes de la intervención. La intervención sólo tuvo efecto en una leve mejora en todos los grupos en la memoria fonológica. Sin embargo, las comparaciones entre los grupos post-intervención mostraron puntuaciones significativamente más bajas en el grupo TDL respecto a los otros dos en fluencia verbal y control inhibitorio, que pueden indicar una posible mejoría de los grupos de dislexia y sin dificultades en estas variables. En conclusión, la intervención tuvo un escaso efecto, especialmente en el grupo con TDL. Se requieren más trabajos con un mayor número de participantes y de sesiones de intervención para poder corroborar la falta de efecto específico sobre el control inhibitorio y la fluencia verbal en los grupos intervenidos.

Reseña de la obra de Rosalind Thomas, Polis Histories, Collective Memories and the Greek World, Cambridge, Cambridge University Press, 2019, 490 pp. [ISBN: 978-1-107-19358-1].

Reseña de la obra de Matthew B. Roller, Models from the Past in Roman Culture. A world of Exempla, Cambridge, Cambridge University Press, 2018, 321 pp. [ISBN: 978-1-316-61490-7].

Reseña de la obra de Karl-Joachim Hölkeskamp, La cultura política de la república romana, un debate historiográfico internacional (=Libera Res Publica 1), Zaragoza, Prensas de la Universidad de Zaragoza–Editorial Universidad de Sevilla, 2019, 242 pp., 6 figs. [ISBN: 978-84-472-2917-8].

Syzygium aromaticum (clove) has been used as a dental analgesic, an anesthetic, and a bioreducing and capping agent in the formation of metallic nanoparticles. The main objective of this study was to evaluate the antimicrobial effect in oral microorganisms of biogenic silver nanoparticles (AgNPs) formed with aqueous extract of clove through an ecofriendly method “green synthesis”. The obtained AgNPs were characterized by UV-Vis (ultraviolet-visible spectroscopy), SEM-EDS (scanning electron microscopy–energy dispersive X-ray spectroscopy), TEM (transmission electron microscopy), and ζ potential, while its antimicrobial effect was corroborated against oral Gram-positive and Gram-negative microorganisms, as well as yeast that is commonly present in the oral cavity. The AgNPs showed absorption at 400–500 nm in the UV-Vis spectrum, had an average size of 4–16 nm as observed by the high-resolution transmission electron microscopy (HR-TEM), and were of a crystalline nature and quasi-spherical form. The antimicrobial susceptibility test showed inhibition zones of 2–4 mm in diameter. Our results suggest that AgNPs synthesized with clove can be used as effective growth inhibitors in several oral microorganisms.

Neonatal appendicitis is an unusual finding with a high mortality rate that occurs in term or preterm newborns. An uncomplicated debut is associated with delayed interventions and diagnosis, resulting in increased mortality. We present a preterm female patient native to a central region of the highlands of Peru who was born at 33 weeks gestation with a birth weight of 1310 g. She was born by cesarean section due to fetal distress and premature detachment of the placenta. She spent four days under CPAP-type respiratory support, and in her first week of life, she was already breastfeeding. At nine days of age, she presented with vomiting, initially resembling dairy but then becoming bilious and like coffee grounds. Imaging studies excluded pyloric hypertrophy and abnormal intestinal transit but resulted in a referral to a tertiary pediatric hospital in Lima to rule out intestinal malrotation. The patient arrived stable at the pediatric hospital at 20 days of life. Imaging studies excluded intestinal malrotation but exhibited a slow intestinal transit. An exploratory surgical intervention scheduled for the fifth day after arrival had to be deferred due to thrombocytopenia and increased prothrombin time, although this condition promptly corrected itself in two days. Finally, a laparotomy at 29 days of age revealed an appendix with vascular congestion and peri-appendicitis. Neonatal appendicitis, in this case, had a recurrent clinical presentation with full recovery after appendectomy. The patient had recurrent signs of intestinal obstruction with alternating periods of clinical and laboratory stability. These recurrent or intermittent clinical manifestations of neonatal appendicitis can delay the diagnosis and contribute to increased mortality.

La producción científica en Ecuador ha seguido un patrón similar a la evolución de otros países latinoamericanos, existiendo escasa generación de documentos indexados; pero en los últimos años se ha incrementado. El INIAP ha plasmado los resultados de sus investigaciones en publicaciones técnicas, dípticos, guías, manuales, entre otros. Desde el año 2014 ha tenido un aumento sostenido en su producción científica, debido a nueva reglamentación de acreditación e incentivos a investigadores. El objetivo de la presente investigación fue realizar un análisis de la producción científica del INIAP durante el periodo 2014-2019. Los resultados mostraron un aumentó 3,1 veces durante el periodo analizado, superando el crecimiento latinoamericano en ese mismo tiempo. Se determinó que más del 54% de las publicaciones del INIAP tienen colaboración internacional y el 62% de publicaciones están indexadas en revistas con índice SJR. Estos resultados indican que INIAP ha incrementado su producción y calidad científica en investigación agropecuaria.