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Mycoplasmas are known as the minimalist microorganisms in the microbes' world. Their minimalist nature makes them highly sensitive to the environmental conditions and limits their ability to survive for extended periods outside their animal host. Nevertheless, there are documented instances of mycoplasma transmission over significant distances and this phenomenon may be linked to relatively unexplored abilities of mycoplasmas, such as their capacity to synthesize biofilm-the predominant mode of bacterial growth in nature. The authors decided to establish a method aimed at inducing the clustering of mycoplasma planktonic cells within a biofilm in vitro and subsequently assess the capacity of certain avian mycoplasmas to synthesize a biofilm. A total of 299 avian mycoplasma isolates were included in the study, encompassing both pathogenic ( Mycoplasma gallisepticum , M. synoviae , M. meleagridis , M. iowae ) and non-pathogenic species ( M. gallinaceum , M. gallinarum , M. iners and M. pullorum) . The authors successfully demonstrated the feasibility of inducing avian mycoplasmas to synthetize in vitro a biofilm, which can be visually quantified. The only species that did not produce any biofilm was M. iowae . In general, the pathogenic mycoplasmas produced greater quantities of biofilm compared to the non-pathogenic ones. Furthermore, it was observed that the ability to produce biofilm appeared to vary, both qualitatively and quantitatively, not only among different species but also among isolates of a single species. Future studies will be necessary to determine whether biofilm production plays a pivotal epidemiological role for the pathogenic avian mycoplasmas., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2024 The Authors.)

Background: In 2021, the Task Force on Breastfeeding of the Italian Ministry of Health released a document calling for the provision of breastfeeding support in case of re-hospitalization of the child after birth. Since type and quality of breastfeeding support during re-hospitalization in Italian Pediatric Units (PUs) is largely unknown, the Breastfeeding Section of the Italian Society of Pediatrics (TASIP) conducted an ad hoc national survey., Methods: In March 2023, a specifically designed electronic questionnaire was sent to the Directors of 328 PUs, who were requested to fill it online., Results: Data from 161 PUs were received, with a response rate of 48.7%. Our results highlighted that 18.6% of units do not provide training on breastfeeding for healthcare professionals and 46% of PUs lack of an ad hoc policy on breastfeeding support in case of re-hospitalization of the child. Although 88.2% of PUs provide breast pumps to the mothers of the re-hospitalized young children, 34.8% lack of a protocol on the storage of expressed breast milk., Conclusions: Breastfeeding support for the mothers of hospitalized breastfed young children appears to be suboptimal in Italian PUs. Interventions aimed to structure and improve the quality of breastfeeding support for the mother-child dyad are needed, particularly developing protocols and providing a training on breastfeeding to the majority of healthcare professionals., (© 2024. The Author(s).)

The aim of the study is to characterize 28 Escherichia coli carbapenem-resistant strains isolated from multi-resistant screening. All the strains were tested through CARBA NP test and PCR analysis for molecular characterization of carbapenemase. Plasmid characterization and phylogenetic study was performed. The molecular characterization revealed that 24 of 28 strains harbour carbapenemases. The most involved plasmids are FIA, FIB, FIIS and FrepB replicons that belong to the IncF group. The phylo-typing analysis revealed a greater presence of the B2 group. Carbapenem resistance in E. coli, should be constantly monitored to avoid the onset of new epidemic episodes.

Social anxiety disorder (SAD) is associated with psychotic-like experiences (PLEs) and is a frequent diagnosis in the prodromal phases of psychosis. We investigated whether psychopathological factors could discriminate which subjects with SAD are more likely to develop PLEs. A sample of 128 young adults with SAD was split into two subsamples according to the presence of clinically relevant PLEs. Correlations between PLEs and other psychopathological markers were explored. The SAD with PLEs group showed higher level of anxiety, depression, and intolerance of uncertainty (IU) compared with the SAD without PLEs group. A limitation of this study is that the cross-sectional design precluded the analysis of causality. In our sample, the presence of PLEs is related to higher levels of depression, anxiety, and IU. The current findings are consistent with hypotheses suggesting that cognitive disturbances, together with social anxiety, may result in PLEs.

Aim: Delays in the admission to care of young adults with emerging mental disorders represent one of the current major concern in psychiatry. This delay, often experienced in clinical practice, has several determinants. One of these is "unexpressed help-seeking" that is influenced by cultural and historical backgrounds and by the characteristics of the disorder itself, but most of all by the way community mental health services are developed. The aims are to identify: level of stress and mental unease and main determinants of unexpressed help-seeking within a community sample of young adults in a national contest of generalist community mental health model., Methods: the sample is made up of 3,446 university students. An explorative questionnaire together with SVS (Stress related Valuation Scale) for the assessment of subjective stress and GHQ-12 for the evaluation of mental health status were given. A descriptive analysis was carried out followed by correlations between unexpressed help-seeking variable and other variables. A logistic regression was carried out on the subsample with GHQ-12 ≥4 utilizing "non help-seeking" as a dependent variable., Results: 46.8% of the sample had a GHQ-12 ≥4 score. The amount of unexpressed help-seeking is equal to 63.6% and ends up being significantly correlated to: male sex, nonresident student, high SVS score, absence of Youth Mental Health Services, distrust manifested in relation to existing Services., Conclusions: A modification of the Community Mental Health Services in the sense of setting up more appropriate contexts for young users, could lead to a reduction of unexpressed help-seeking.

Aim: This study aimed to investigate if particular psychotic-like experiences (PLEs) subtypes were more likely to be associated with a series of socio-demographic variables, with alcohol abuse and with cannabis or illicit drug use. The idea is to further characterize different PLEs subtypes in order to discloud their individual nature., Methods: A cross-sectional design was conducted on a sample of 997 university students aged between 19 and 26 years, which belonged to 4 faculties of 2 different universities. Alcohol abuse and cannabis or illicit drug use were assessed using a self-report questionnaire; PLEs were assessed using the Community Assessment of Psychic Experiences. Cases were randomized in order to obtain equipotent groups; then, an analysis of the probable dependence relations between PLEs subtypes and other variables was conducted using analysis of variance models., Results: Persecutory ideas (PI) and bizarre experiences were more frequent within females and non-resident students, moreover PI were more frequent within younger subjects (<20 years). Magical thinking was significantly associated to a more frequent cannabis use in the last year (>1/month)., Conclusions: These results confirm usefulness and validity of a "subtype approach" to PLEs. In fact PLEs subtypes may have, not only a different clinical presentation, but even different epidemiological and psychopathological ones.

Background: Different subtypes of psychotic experiences (PEs) have been identified in clinical and non-clinical samples. Researchers have considered these PEs to either be variations of personality or expressions of vulnerability to psychotic disorder. This study aimed to determine which particular subtypes of PEs were more likely to be associated with poor mental health status and help-seeking behaviour in a non-clinical sample of young adults., Methods: The study was conducted on a community sample of 997 young adults. The prevalence of PEs and distress was measured using the community assessment of psychic experiences (CAPE), depressive and anxiety symptoms were measured using Beck depression inventory-II and Beck anxiety inventory, and general functioning was measured using the general health questionnaire-12. Factorial analysis of the CAPE positive dimension was conducted and correlations between factors and clinical variables were analysed., Results: Four PE subtypes were identified: perceptual abnormalities, persecutory ideas (PI), bizarre experiences, and magical thinking. At least one high frequency PI was endorsed by 60.8% (n = 606) of the sample and proved to be significantly associated both with poor mental health status and help-seeking behaviour., Conclusion: PEs subtypes are differentially associated with various markers of poor mental health status. PI seem to have stronger psychopathological significance than other subtypes of PEs. Further longitudinal studies are required to extend these findings., (© 2011 Blackwell Publishing Asia Pty Ltd.)

There is ample evidence for the interest of screening for psycho-active substance use in primary care, coupled with a brief intervention. The screening can be done during the medical consultation (focusing on frequency and quantity of substance use, loss of control and medical and psychosocial consequences) or with standardised screening instruments. The use of a questionnaire can be helpful for the physician. This article describes some validated questionnaires that allow a rapid and non-stigmatising screening for use of one or several substances. The ASSIST questionnaire, developed by WHO, accessible online, can be particularly useful; it allows the screening, evaluation and orientation for substance use and related problems in primary and general medical care settings.

Aims: To evaluate: (i) self-perceived stress, depressive and anxiety symptoms in a community sample of young adults; (ii) which elements have most impact on the development of mental disease; (iii) if high levels of mental disease are related to help-seeking behavior., Materials and Methods: The study was carried out on a sample of 1660 university students. The evaluation of the perceived stress was conducted using the Stress-Related Vulnerability Scale (SVS), the evaluation of depressive and anxiety symptoms with Beck Depression Inventory-II (BDI-II) and Beck Anxiety Inventory (BAI). A descriptive analysis of all the data collected was carried out and the principal links between variables and level of mental disease were detected. Finally the reasons for the failure to seek help were investigated., Results: Mean scores of BDI-II and BAI were respectively 10.9 (sigma = 8.3) and 11.4 (sigma = 9.4) and about 4% of the sample showed a pathological level of stress with the SVS. Female sex, non resident status and conflictual family climate were found to be more related with more severe anxious and depressive symptoms. Moreover, the latter ones were found to be strongly related with help-seeking behavior., Conclusion: In this sample mental disease associated with distress show a significant percentage. Higher level of anxiety and depressive symptoms are more related to perceived need for help and help-seeking behavior. Nevertheless the level of unexpressed help-seeking turn out to be high for multiple reason.