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13 results on '"Urselli, Francesca"'

3. Production and use of antigen tetramers to study antigen-specific B cells

Author

University of Texas, University of Minnesota, Ontario Graduate Scholarship, Universidad Politécnica de Madrid, Ministerio de Ciencia e Innovación (España), National Institutes of Health (US), Phelps, Allyssa [0000-0002-5025-7459], Pazos-Castro, Diego [0000-0002-0686-5131], Urselli, Francesca [0000-0002-5839-7384], Fang, Allison [0000-0002-5865-8695], Tome-Amat, Jaime [0000-0003-4442-3649], Díaz-Perales, Araceli [0000-0002-1093-3627], Waserman, Susan [0000-0002-2136-5995], Boonyaratanakornkit, Jim [0000-0001-5858-2662], Jordana, Manel [0000-0002-5861-5994], Taylor, Justin J. [0000-0001-9718-2901], Koenig, Joshua [0000-0002-8909-5039], Phelps, Allyssa, Pazos-Castro, Diego, Urselli, Francesca, Grydziuszko, Emily, Mann-Delany, Olivia, Fang, Allison, Walker, Tina D, Guruge, Rangana Talpe, Tome-Amat, Jaime, Díaz-Perales, Araceli, Waserman, Susan, Boonyaratanakornkit, Jim, Jordana, Manel, Taylor, Justin J., Koenig, Joshua, University of Texas, University of Minnesota, Ontario Graduate Scholarship, Universidad Politécnica de Madrid, Ministerio de Ciencia e Innovación (España), National Institutes of Health (US), Phelps, Allyssa [0000-0002-5025-7459], Pazos-Castro, Diego [0000-0002-0686-5131], Urselli, Francesca [0000-0002-5839-7384], Fang, Allison [0000-0002-5865-8695], Tome-Amat, Jaime [0000-0003-4442-3649], Díaz-Perales, Araceli [0000-0002-1093-3627], Waserman, Susan [0000-0002-2136-5995], Boonyaratanakornkit, Jim [0000-0001-5858-2662], Jordana, Manel [0000-0002-5861-5994], Taylor, Justin J. [0000-0001-9718-2901], Koenig, Joshua [0000-0002-8909-5039], Phelps, Allyssa, Pazos-Castro, Diego, Urselli, Francesca, Grydziuszko, Emily, Mann-Delany, Olivia, Fang, Allison, Walker, Tina D, Guruge, Rangana Talpe, Tome-Amat, Jaime, Díaz-Perales, Araceli, Waserman, Susan, Boonyaratanakornkit, Jim, Jordana, Manel, Taylor, Justin J., and Koenig, Joshua

Abstract

B cells generate antibodies that provide protection from infection, but also cause pathology in autoimmune and allergic conditions. Antigen-specific B cells can be detected by binding their surface antibody receptors with native antigens conjugated to fluorescent probes, a technique that has revealed substantial insight into B cell activation and function. This protocol describes the process of generating fluorescent antigen tetramer probes and delineates a process of enriching large samples based on antigen-specificity for high-resolution analyses of the antigen-specific B cell repertoire. Enrichment of tetramer-binding cells allows for detection of antigen-specific B cells as rare as 1 in 100 million cells, providing sufficient resolution to study naive B cells and IgE-expressing cells by flow cytometry. The generation of antigen tetramers involves antigen biotinylation, assessment of biotin:antigen ratio for optimal tetramer loading and polymerization around a streptavidin-fluorophore backbone. We also describe the construction of a control tetramer to exclude B cells binding to the tetramer backbone. We provide a framework to validate whether tetramer probes are detecting true antigen-specific B cells and discuss considerations for experimental design. This protocol can be performed by researchers trained in basic biomedical/immunological research techniques, using instrumentation commonly found in most laboratories. Constructing the antigen and control tetramers takes 9 h, though their specificity should be assessed before experimentation and may take weeks to months depending on the method of validation. Sample enrichment requires ~2 h but is generally time and cost neutral as fewer cells are run through the flow cytometer.

Published
2024

4. NDP52 acts as a redox sensor in PINK1/Parkin‐mediated mitophagy

Author

Kataura, Tetsushi, Otten, Elsje G, Rabanal‐Ruiz, Yoana, Adriaenssens, Elias, Urselli, Francesca, Scialo, Filippo, Fan, Lanyu, Smith, Graham R, Dawson, William M, Chen, Xingxiang, Yue, Wyatt W, Bronowska, Agnieszka K, Carroll, Bernadette, Martens, Sascha, Lazarou, Michael, and Korolchuk, Viktor I

Published
2023
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7. NDP52 acts as a redox sensor in PINK1/Parkin‐mediated mitophagy

Author

Kataura, Tetsushi, primary, Otten, Elsje G, additional, Rabanal‐Ruiz, Yoana, additional, Adriaenssens, Elias, additional, Urselli, Francesca, additional, Scialo, Filippo, additional, Fan, Lanyu, additional, Smith, Graham R, additional, Dawson, William M, additional, Chen, Xingxiang, additional, Yue, Wyatt W, additional, Bronowska, Agnieszka K, additional, Carroll, Bernadette, additional, Martens, Sascha, additional, Lazarou, Michael, additional, and Korolchuk, Viktor I, additional

Published
2022
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9. Autophagy promotes cell and organismal survival by maintaining NAD(H) pools

Author

Sedlackova, Lucia, primary, Otten, Elsje G., additional, Scialo, Filippo, additional, Shapira, David, additional, Kataura, Tetsushi, additional, Carroll, Bernadette, additional, Seranova, Elena, additional, Rabanal-Ruiz, Yoana, additional, Kelly, George, additional, Stefanatos, Rhoda, additional, Nelson, Glyn, additional, Urselli, Francesca, additional, Acharjee, Animesh, additional, Kenneth, Niall, additional, Trushin, Sergey, additional, Zhang, Tong, additional, Bascom, Charles C., additional, Tasseff, Ryan, additional, Isfort, Robert J., additional, Oblong, John E., additional, Trushina, Eugenia, additional, Imoto, Masaya, additional, Saiki, Shinji, additional, Lazarou, Michael, additional, Papamichos Chronakis, Manolis, additional, Maddocks, Oliver D.K., additional, Sarkar, Sovan, additional, Sanz, Alberto, additional, and Korolchuk, Viktor I., additional

Published
2020
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10. New Platinum-Based Prodrug Pt(IV)Ac-POA: Antitumour Effects in Rat C6 Glioblastoma Cells

Author

Ferrari, Beatrice, primary, Urselli, Francesca, additional, Gilodi, Martina, additional, Camuso, Serena, additional, Priori, Erica Cecilia, additional, Rangone, Beatrice, additional, Ravera, Mauro, additional, Veneroni, Paola, additional, Zanellato, Ilaria, additional, Roda, Elisa, additional, Osella, Domenico, additional, and Bottone, Maria Grazia, additional

Published
2019
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13. Active Moderate-to-Severe Graves' Orbitopathy in a Patient With Type 2 Diabetes Mellitus and Vascular Complications

Author

Livia Peschi, Gilda Pontieri, Anna Rita Ruggiero, Bernadette Biondi, Alessia Liccardi, Francesca Urselli, Urselli, Francesca, Pontieri, G, Peschi, L, Liccardi, A, Ruggiero, A R, and Biondi, B

Subjects
Pediatrics, medicine.medical_specialty, Exophthalmos, Endocrinology, Diabetes and Metabolism, Trab, Physical examination, Case Report, Type 2 diabetes, lcsh:Diseases of the endocrine glands. Clinical endocrinology, methotrexate, Graves' orbitopathy, tocilizumab, Endocrinology, Diabetes mellitus, medicine, hyperthyroidism, Risk factor, radiotherapy, lcsh:RC648-665, medicine.diagnostic_test, glucocorticoids, business.industry, Type 2 Diabetes Mellitus, hyperthyroidism, diabetes mellitus, Graves’ orbitopathy, glucocorticoids, methotrexate, tocilizumab, radiotherapy, medicine.disease, diabetes mellitus, medicine.symptom, Thyroid function, business
Abstract

Background: Graves’ orbitopathy (GO) is the main extrathyroidal manifestation of Graves’ disease (GD). Diabetes mellitus (DM) has been reported to be a risk factor in patients with GO. Moreover, GO can be more frequent and severe in type 2 diabetes patients. High doses of intravenous glucocorticoids represent the first line treatment of moderate-to-severe and active GO according to the international guidelines. However, this therapy is contraindicated in uncontrolled diabetes and in patients with increased cardiovascular risk. Some anti-diabetic drugs can exacerbate GO. We reported the clinical case of an active and moderate-to-severe GO in a patient with uncontrolled type 2 DM and vascular complications. Case Report: A 61-years-old patient came to our ambulatory for a recurrence of GD and a moderate-to-severe bilateral GO. The patient had uncontrolled type 2 DM during insulin therapy and a history of micro and macrovascular complications. At the physical examination, the clinical activity score was 5 and the severity of GO was moderate-to-severe. A blood sample showed overt hyperthyroidism and the persistence of anti-TSH receptor antibodies (TRAb) during treatment with methimazole. A computed tomography scan showed a moderate-to-severe bilateral exophthalmos. We discuss the benefit/risk of treatment of GO in our patient. Conclusion: The available guidelines do not focus on the treatment of diabetic patients with uncontrolled diabetes and severe vascular complications, therefore our patient represents a difficult therapeutic challenge. The screening of thyroid function and the evaluation of GO could be useful in diabetic patients with autoimmune thyroid disease to perform a correct treatment of these disorders.

Published
2019

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