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Aim: The aim of the study was to assess and evaluate the antimicrobial effectiveness of chitosan nanoparticles (CSNPs) with calcium hydroxide in the elimination of Enterococcus faecalis. Materials and Methods: Using the broth microdilution method, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of calcium hydroxide and CSNPs were measured. The antibiofilm effect of calcium hydroxide and CSNPs against E. faecalis biofilm was qualitatively analyzed using a crystal violet assay. A 7-day-old biofilms of E. faecalis grown on dentine discs were assigned to the following three groups (n = 11 dentine discs), normal saline (group I), calcium hydroxide (group II), and CSNPs (group III). Quantification of live and dead cells using confocal microscopy was done to evaluate the antibiofilm efficacy of the medicaments included in the study. Results: MIC of calcium hydroxide and CSNPs against E. faecalis was observed at 2.5 mg/mL and 0.31 mg/mL, respectively. MBC of calcium hydroxide and CSNPs was observed at 2.5 mg/mL and 0.31 mg/mL, respectively. Using Crystal Violet (CV) assay, calcium hydroxide and CSNPs showed biofilm inhibition at concentrations of 2.5 mg/mL and 0.625 mg/mL, respectively. Confocal laser scanning microscopy analysis found that both calcium hydroxide and CSNPs showed a significant decrease in viable cells at their MBC values compared to the control group's normal saline. CSNPs showed a significantly lower percentage of live cells than calcium hydroxide (P < 0.05). Conclusion: The study results reveal that the antimicrobial efficacy of CSNPs is better than calcium hydroxide and normal saline against E. faecalis biofilm. [ABSTRACT FROM AUTHOR]

Introduction: Gonial angle is an important parameter of the craniofacial complex giving an indication about the vertical parameters and symmetry of the facial skeleton. Both orthopantomogram (OPG) and lateral cephalograms can be used for the measurement of gonial angle. Because of the superimpositions seen on lateral cephalograms, reliable measurement of the gonial angle becomes difficult. The aim of the present study is to check the possible application and reliability of OPG for gonial angle determination by clarifying whether there is any signifi cant difference between the determination of gonial angle from OPG and cephalogram. Materials and Methods: Gonial angle measurements were made on lateral cephalograms and orthopantomograms of 98 patients - 44 males (mean age 25.9 years) and 54 females (mean age 21.3 years), and compared using Statistical Package for Social Sciences. Results: One-way analysis of variance demonstrated no significant differences between the values of gonial angles determined by lateral cephalogram and panoramic radiography. Pearson correlation showed a high correlation between cephalometric and OPG gonial angle value. Conclusion: Panoramic radiography can be used to determine the gonial angle as accurately as a lateral cephalogram. For determination of the gonial angle, an OPG may be a better choice than a lateral cephalogram as there are no interferences due to superimposed images of anatomical structures as in a lateral cephalogram. Thus, the present study substantiates the possibility of enhancing the clinical versatility of the panoramic radiograph, which is an indispensable tool for dental diagnosis. [ABSTRACT FROM AUTHOR]

Aim: This in vitro study aims to detect the dentinal defects associated with different irrigants during biomechanical preparation using rotary nickel-titanium (Ni-Ti) files (Hyflex CM)., Materials and Methods: For the study, 120 extracted mandibular incisors were taken. Depending on the irrigant used, teeth were divided into six groups ( n = 20). In Group 1, teeth were not biomechanically prepared considered as, control group. In Group 2, Group 3, Group 4, Group 5, and Group 6, biomechanical preparation was with Hyflex CM rotary Ni-Ti files using saline, 3% sodium hypochlorite (NaOCl), 17% ethylenediaminetetraacetic acid (EDTA), SmearClear, and 2% chlorhexidine (CHX), respectively, as irrigant. IsoMet Saw was used to section samples at 3, 6, and 9 mm from the apex perpendicular to the long axis of tooth. Segments were seen at 30x magnification under a digital stereomicroscope., Statistical Analysis: Statistical analysis was done using two-tailed t -test., Results: Maximum number of dentinal defects was seen with 17% EDTA, followed by 3% NaOCl, SmearClear, and 2% CHX., Conclusions: Irrigation with 17% EDTA causes more dentinal defects as compared to irrigation with 3% NaOCl, SmearClear, and 2% CHX., Competing Interests: There are no conflicts of interest., (Copyright: © 2022 Journal of Conservative Dentistry.)