16 results on '"Upadhyay NK"'
Search Results
2. Bilosomes in brain drug delivery.
- Author
-
Kaurav H, Kapoor DN, and Upadhyay NK
- Subjects
- Brain, Administration, Oral, Drug Delivery Systems, Liposomes
- Published
- 2023
- Full Text
- View/download PDF
3. Surface engineering of colloidal quaternary chalcogenide Cu 2 ZnSnS 4 nanocrystals: a potential low-cost photocatalyst for water remediation.
- Author
-
Semalti P, Sharma V, Devi M, Prathap P, Upadhyay NK, and Sharma SN
- Subjects
- Water, Butylamines, Ligands, Solvents, Environmental Pollutants, Nanoparticles
- Abstract
Colloidal route synthesis of quaternary compound CZTS (Cu
2 ZnSnS4 ) has been anticipated with an inimitable combination of coordinating ligands and solvents using the hot injection technique. CZTS is recognized as one of the worthiest materials for photo-voltaic/catalytic applications due to its exclusive properties (viz., non-toxic, economical, direct bandgap, high absorbance coefficient, etc.). This paper demonstrates the formation of crystalline, single-phased, monodispersed, and electrically passivated CZTS nanoparticles using a distinctive combination of ligands viz. oleic acid (OA)-trioctylphosphine (TOP) and butylamine (BA)-trioctylphosphine (TOP). Detailed optical, structural, and electrochemical studies were done for all CZTS nanoparticles, and the most efficient composition was found using ligands butylamine and TOP. CZTS nanocrystals were rendered hydrophilic via surface-ligand engineering, which was used for photocatalysis studies of organic pollutants. Malachite green (MG) and rhodamine 6G (Rh) for water remediation have great commercial prospects. The unique selling proposition of this work is the rapid synthesis time (~ 45 min) of colloidal CZTS nanocrystals, cost-effective ligand-exchange process, and negligible material wastage (~ 200 µl per 10 ml of pollutant) during photocatalytic experiments., (© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)- Published
- 2023
- Full Text
- View/download PDF
4. Studies of Phytochemicals, Antioxidant, and Antibacterial Activities of Pinus gerardiana and Pinus roxburghii Seed Extracts.
- Author
-
Bhardwaj K, Sharma R, Cruz-Martins N, Valko M, Upadhyay NK, Kuča K, and Bhardwaj P
- Subjects
- Anti-Bacterial Agents pharmacology, Hydrogen Peroxide analysis, Phytochemicals analysis, Phytochemicals pharmacology, Plant Extracts chemistry, Seeds chemistry, Antioxidants chemistry, Pinus
- Abstract
Pine seeds are considered as nonwood forest products (NWFP) with regularly increasing market's demand. They can be eaten in various ways such as roasted or raw. In addition, they are included in various traditional dishes like in cookies, sauces, candies, cakes, breads, and other bakery items and, moreover, for medicinal purposes. GC-MS study is performed to analyze the phytochemical compounds present in the seed extracts of Pinus roxburghii (Chir) and Pinus gerardiana (Chilgoza). In total, 25 compounds were identified each in Chir and Chilgoza. In Chir seeds, abundantly present compounds were 2,4-di-tert-butylphenol (16.6%), followed by ç-Terpinene (9.9%) and cyclohexanol, 4-ethenyl-4-methyl-3-(1-methylethenyl)-, (1à,3à,4á) (9.8%), whereas in Chilgoza seeds, the maximum amount of compound was 1-hexyl-1-nitrocyclohexane (17.3%), followed by phenol, 2,6-bis(1,1-dimethylethyl) (15.4%), and heptadecane, 2-methyl (8.4%). The total phenolic content of Chir seed sample was 1536 ± 4.35 (mg GAE/100 g), whereas in the Chilgoza seed extract was 642.66 ± 2.08 (mg GAE/100 g). The application of RP-HPLC-DAD system revealed that Chir and Chilgoza seeds have maximum quantity of catechin (15.77 ± 0.16 μ g/mg and 17.49 ± 0.32 μ g/mg, respectively). Both Chir and Chilgoza seed extracts exhibited significant antioxidant (radical scavenging) potential, through H
2 O2 (618.94 ± 21.45 μ g/mL and 575.16 ± 19.88 μ g/mL) and DPPH (552.60 ± 13.03 μ g/mL and 429.15 ± 3.80 μ g/mL) assays, respectively. Additionally, a well-known antibacterial potential was also found in both plants' dichloromethane extracts, with 64 to 256 μ g/mL of minimum inhibitory concentrations. As a whole, result shows the importance of both plants as a naturally occurring phytochemical source with significant antibacterial and antioxidant activity., Competing Interests: The authors have no conflicts of interest to declare., (Copyright © 2022 Kanchan Bhardwaj et al.)- Published
- 2022
- Full Text
- View/download PDF
5. Conifers Phytochemicals: A Valuable Forest with Therapeutic Potential.
- Author
-
Bhardwaj K, Silva AS, Atanassova M, Sharma R, Nepovimova E, Musilek K, Sharma R, Alghuthaymi MA, Dhanjal DS, Nicoletti M, Sharma B, Upadhyay NK, Cruz-Martins N, Bhardwaj P, and Kuča K
- Subjects
- Animals, Clinical Trials as Topic, Humans, Neuroprotection drug effects, Phytochemicals chemistry, Plant Extracts therapeutic use, Forests, Phytochemicals therapeutic use, Tracheophyta chemistry
- Abstract
Conifers have long been recognized for their therapeutic potential in different disorders. Alkaloids, terpenes and polyphenols are the most abundant naturally occurring phytochemicals in these plants. Here, we provide an overview of the phytochemistry and related commercial products obtained from conifers. The pharmacological actions of different phytochemicals present in conifers against bacterial and fungal infections, cancer, diabetes and cardiovascular diseases are also reviewed. Data obtained from experimental and clinical studies performed to date clearly underline that such compounds exert promising antioxidant effects, being able to inhibit cell damage, cancer growth, inflammation and the onset of neurodegenerative diseases. Therefore, an attempt has been made with the intent to highlight the importance of conifer-derived extracts for pharmacological purposes, with the support of relevant in vitro and in vivo experimental data. In short, this review comprehends the information published to date related to conifers' phytochemicals and illustrates their potential role as drugs.
- Published
- 2021
- Full Text
- View/download PDF
6. Determination of Colistin B in Chicken Muscle and Egg Using Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry.
- Author
-
Kumar H, Kumar D, Nepovimova E, Oulkar D, Kumar A, Azad RMR, Budakoti SK, Upadhyay NK, Verma R, and Kuča K
- Subjects
- Animals, Anti-Bacterial Agents, Chickens, Chromatography, High Pressure Liquid, Chromatography, Liquid, India, Muscles, Solid Phase Extraction, Colistin, Tandem Mass Spectrometry
- Abstract
Colistin, an imperative member of the polymyxin group, is a cationic peptide antibiotic. Itis also known as polymyxin E, but this peptide antibiotic has been forbidden for human consumption due to its high toxicity. Regrettably, this antibiotic is utilized as a feed additive and veterinary drug for animals. Due to the toxicity of colistin, the presence of its residue in the animal system represents a threat to human health regarding the consumption of meat, especially chicken. A novel method was proposed for quantifying colistin B in chicken muscles and eggs using ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). In this method, extraction of colistin B from samples was achieved by mixing the sample with acidified methanol:water (1/1, v / v ), followed by centrifugation and filtration by a membrane filter excluding solid-phase extraction (SPE) clean up, as well as evaporation steps. The analysis was conducted by optimized liquid chromatography-tandem mass spectrometry (LC-MS/MS), and method performance was assessed in terms of the limit of quantitation, specificity, selectivity, precision, linearity and recovery in coherence with the guidelines of SANTE and the Commission Decision 2002/657/EC. The result obtained from the study showed the limit of quantitation (LOQ) as 10 µg Kg
-1 for muscles and 5 µg Kg-1 for eggs, with acceptable recoveries along with precision. The linearity was plotted in the range of 5-25 µg L-1 (solvent) for egg and 10-50 µg Kg-1 (matrix-matched) for muscles. The result of average recoveries showed the value of 70-94% (3.3-12% relative standard deviation (RSD)) for chicken muscles and 88-107% (2.5-18.6% RSD) for egg samples, which meets the criteria for acceptability of method according to both SANTE and 2002/657/EC guidelines. This proposed protocol provides a cost-effective solution for food testing labs by reducing the cost of the sample preparation by 60% along with the time required for SPE cleanup. Further, the optimized method was also tested on real samples collected from nearby provinces in Solan city, Himachal Pradesh, India, and three out of 20 muscles were found to have colistin B in the range of 50-560 µg Kg-1 .- Published
- 2021
- Full Text
- View/download PDF
7. Understanding of Colistin Usage in Food Animals and Available Detection Techniques: A Review.
- Author
-
Kumar H, Chen BH, Kuca K, Nepovimova E, Kaushal A, Nagraik R, Bhatia SK, Dhanjal DS, Kumar V, Kumar A, Upadhyay NK, Verma R, and Kumar D
- Abstract
Progress in the medical profession is determined by the achievements and effectiveness of new antibiotics in the treatment of microbial infections. However, the development of multiple-drug resistance in numerous bacteria, especially Gram-negative bacteria, has limited the treatment options. Due to this resistance, the resurgence of cyclic polypeptide drugs like colistin remains the only option. The drug, colistin, is a well-known growth inhibitor of Gram-negative bacteria like Acinetobacter baumanni , Enterobacter cloacae , Klebsiella pneumoniae , and Pseudomonas aeruginosa. Technological advancements have uncovered the role of the mcr-1 (mobilized colistin resistance) gene, which is responsible for the development of resistance in Gram-negative bacteria, which make them distinct from other bacteria without this gene. Additionally, food animals have been determined to be the reservoir for colistin resistance microbes, from which they spread to other hosts. Due to the adverse effects of colistin, many developed countries have prohibited its usage in animal foods, but developing countries are still using colistin in animal food production, thereby imposing a major risk to the public health. Therefore, there is a need for implementation of sustainable measures in livestock farms to prevent microbial infection. This review highlights the negative effects (increased resistance) of colistin consumption and emphasizes the different approaches used for detecting colistin in animal-based foods as well as the challenges associated with its detection.
- Published
- 2020
- Full Text
- View/download PDF
8. Bioassay guided fractionation of rhizome extract of Rheum emodi wall as bio-availability enhancer of antibiotics against bacterial and fungal pathogens.
- Author
-
Rolta R, Kumar V, Sourirajan A, Upadhyay NK, and Dev K
- Subjects
- Anti-Bacterial Agents isolation & purification, Antifungal Agents isolation & purification, Bacteria growth & development, Candida albicans growth & development, Chemical Fractionation, Dose-Response Relationship, Drug, Drug Synergism, Methanol chemistry, Microbial Sensitivity Tests, Plant Extracts isolation & purification, Solvents chemistry, Anti-Bacterial Agents pharmacology, Antifungal Agents pharmacology, Bacteria drug effects, Biological Assay, Candida albicans drug effects, Emodin chemistry, Plant Extracts pharmacology, Rhizome chemistry
- Abstract
Ethnopharmacological Relevance: Rheum emodi Wall., is an important medicinal plant extensively used in Ayurvedic and Unani systems of traditional medicine. It is known to possess antioxidant, antibacterial, antifungal, anticancer, wound healing and immune enhancing activities., Aim of the Study: The aim of the current study was to investigate the antimicrobial activity and synergistic potential of different solvent fractions and phytocompounds of Rheum emodi rhizome against bacterial and fungal pathogens., Material and Methods: The antimicrobial and synergistic potential of the crude methanolic extract, different solvent fractions (n-hexane, chloroform, ethyl acetate, and residual aqueous) and isolated phytocompounds of the rhizome of Rheum emodi were assayed by broth microdilution method. The bioactive phytocompounds were isolated through silica TLC and quantified using HPTLC and HPLC. The bioactive phytocompounds were identified by LC-MS analysis., Results: Phytochemical analysis of the sub-fractions showed that the TPC (417.94 ± 1.2 mg g
-1 GAE) and TFC (187.40 ± 0.5 mg g-1 RE) were highest in residual aqueous extracts. The chloroform sub-fraction possessed the highest antimicrobial activity against bacterial (Escherichia coli, Staphylococcus aureus and Klebsiella pneumoniae) and fungal strains (Candida albicans MTCC 277 and ATCC 90028). The MIC of chloroform sub-fraction against S. aureus, K. pneumoniae, E. coli, C. albicans was 1.95, 3.91, 15.62 and 62.5 μg ml-1 , respectively. TLC and LC-MS analysis of chloroform sub-fraction identified phytocompounds namely emodin D4 (m/z 274.262), rhein13c6 (m/z 290.176), chrysophanol dimethyl ether (m/z 282.291), and resveratrol (m/z 340.456). Quantification of emodin content showed that the chloroform sub-fraction (101.4543 μg mg-1 , 194.8037 μg mg-1 measured through HPTLC and HPLC, respectively), and its TLC fraction (II) (75.18 μg mg-1 , 232.384 μg mg-1 measured through HPTLC and HPLC, respectively) are rich in emodin. Furthermore, chloroform sub-fraction, its TLC fractions and emodin showed profound synergistic activity in combination with antibacterial and antifungal antibiotics and lowered the dosage of antibiotics by 4-257 folds., Conclusions: The bioassay guided fractionation of R. emodi rhizome methanolic extract identified phytocompounds (emodin, rhein13c6, chrysophanol dimethyl ether and resveratrol) that act as bioavailability enhancers of antibacterial and antifungal antibiotics, hence revealing their potential in treating multidrug resistance., (Copyright © 2020 Elsevier B.V. All rights reserved.)- Published
- 2020
- Full Text
- View/download PDF
9. An artificial spiking afferent nerve based on Mott memristors for neurorobotics.
- Author
-
Zhang X, Zhuo Y, Luo Q, Wu Z, Midya R, Wang Z, Song W, Wang R, Upadhyay NK, Fang Y, Kiani F, Rao M, Yang Y, Xia Q, Liu Q, Liu M, and Yang JJ
- Subjects
- Equipment Design, Mechanoreceptors, Niobium chemistry, Oxides chemistry, Titanium chemistry, Afferent Pathways, Neural Networks, Computer, Neural Prostheses, Robotics instrumentation
- Abstract
Neuromorphic computing based on spikes offers great potential in highly efficient computing paradigms. Recently, several hardware implementations of spiking neural networks based on traditional complementary metal-oxide semiconductor technology or memristors have been developed. However, an interface (called an afferent nerve in biology) with the environment, which converts the analog signal from sensors into spikes in spiking neural networks, is yet to be demonstrated. Here we propose and experimentally demonstrate an artificial spiking afferent nerve based on highly reliable NbO
x Mott memristors for the first time. The spiking frequency of the afferent nerve is proportional to the stimuli intensity before encountering noxiously high stimuli, and then starts to reduce the spiking frequency at an inflection point. Using this afferent nerve, we further build a power-free spiking mechanoreceptor system with a passive piezoelectric device as the tactile sensor. The experimental results indicate that our afferent nerve is promising for constructing self-aware neurorobotics in the future.- Published
- 2020
- Full Text
- View/download PDF
10. Nitrogen donor ligand for capping ZnS quantum dots: a quantum chemical and toxicological insight.
- Author
-
Pandey V, Tripathi VK, Singh KK, Bhatia T, Upadhyay NK, Goyal B, Pandey G, Hwang I, and Tandon P
- Abstract
Nanoparticles having strong optical and electronic properties are the most widely used materials in sensor development. Since the target analyte interacts directly with the surface of the material, the choice of ligand for functionalizing the surface of the material is the key for its further applications. The functionalized surface of the material makes it suitable for required applications as it controls the size of the particle during its growth from the solution phase. Biomolecule capped nanomaterials are favourable for various applications in bio-sensing. In the present work, an attempt has been made to explore the biologically active molecule imidazole as capping agent for ZnS semiconductor nanoparticles or quantum dots (QDs). This work explores the possibility of replacing conventional thiol-zinc bonding and hence paves new pathways for biomolecules having the possibility of being efficient capping agents. Computational chemistry has been used to study the mechanism of bonding between one of the nitrogen atoms of imidazole and the zinc ion of the ZnS QDs. The quantum chemical insight not only explores the most spontaneous interaction of zinc ion and imidazole molecule so as to act as an efficient capping agent but also explains the probable bonding site for nitrogen-zinc chemistry. The tailormade Mn doped ZnS QDs are one of the most promising materials for probe and sensor development. The ZnS core having non-toxicity and the emission in longer wavelength due to manganese makes this material highly useful biologically. The aqueous route of synthesis has been employed to obtain a highly homogeneous and pure material which was further characterized by UV (Ultra Violet spectroscopy), Spectrofluorometer, Transmission Electron Microscope and X-ray Diffraction. The toxicity at the cellular and genetic levels was also investigated to prove the potential of the imidazole capped Mn doped ZnS QD as a biocompatible material., Competing Interests: This work does not involve any conflict of interest and all the authors have given their consent., (This journal is © The Royal Society of Chemistry.)
- Published
- 2019
- Full Text
- View/download PDF
11. Capacitive neural network with neuro-transistors.
- Author
-
Wang Z, Rao M, Han JW, Zhang J, Lin P, Li Y, Li C, Song W, Asapu S, Midya R, Zhuo Y, Jiang H, Yoon JH, Upadhyay NK, Joshi S, Hu M, Strachan JP, Barnell M, Wu Q, Wu H, Qiu Q, Williams RS, Xia Q, and Yang JJ
- Abstract
Experimental demonstration of resistive neural networks has been the recent focus of hardware implementation of neuromorphic computing. Capacitive neural networks, which call for novel building blocks, provide an alternative physical embodiment of neural networks featuring a lower static power and a better emulation of neural functionalities. Here, we develop neuro-transistors by integrating dynamic pseudo-memcapacitors as the gates of transistors to produce electronic analogs of the soma and axon of a neuron, with "leaky integrate-and-fire" dynamics augmented by a signal gain on the output. Paired with non-volatile pseudo-memcapacitive synapses, a Hebbian-like learning mechanism is implemented in a capacitive switching network, leading to the observed associative learning. A prototypical fully integrated capacitive neural network is built and used to classify inputs of signals.
- Published
- 2018
- Full Text
- View/download PDF
12. In-vitro osteoblast proliferation and in-vivo anti-osteoporotic activity of Bombax ceiba with quantification of Lupeol, gallic acid and β-sitosterol by HPTLC and HPLC.
- Author
-
Chauhan S, Sharma A, Upadhyay NK, Singh G, Lal UR, and Goyal R
- Subjects
- Animals, Body Weight drug effects, Chromatography, High Pressure Liquid, Chromatography, Thin Layer, Female, Gallic Acid analysis, Ovariectomy, Pentacyclic Triterpenes analysis, Plant Extracts chemistry, Plant Extracts pharmacology, Rats, Rats, Wistar, Sitosterols analysis, Bombax chemistry, Cell Proliferation drug effects, Gallic Acid pharmacology, Osteoblasts drug effects, Osteoporosis metabolism, Pentacyclic Triterpenes pharmacology, Sitosterols pharmacology
- Abstract
Background: Bombax ceiba is used traditionally to treat bone disorders, rheumatism, and joint pain. The aim of the study is to carry out osteogenic activity in-vitro and anti-osteoporotic activity in-vivo of stem bark of B. ceiba in surgical ovariectomy model in female rats., Methods: Plant drug: B. ceiba stem bark was extracted with solvents petroleum ether and methanol using Soxhlet extraction. In-vitro osteoblastic proliferation study was performed using UMR-106 cell lines. Both the extracts were undergone to acute toxicity study as per OECD423 guidelines. Female Wistar albino rats 180-240 g were used (n = 6). Surgical ovariectomy was performed under anesthesia to induce bone porosity and loss in all animals except normal control and sham control. Each extract was administered at two dose level: 100 and 200 mg/kg and the standard Raloxifene was given at 1 mg/kg orally for 28 days. The phytochemical study of both the extracts was performed using HPLC and HPTLC., Results: A significant osteoblast cell proliferation and alkaline phosphatase activity were observed with B. ceiba extracts in UMR-106 cell lines. Surgical removal of ovaries produced significant (p < 0.05) decline in bone mineral density, bone breaking strength, serum ALP, calcium, phosphorus, and estradiol level and marked bone tissue destruction in histology. Administration of petroleum ether and methanolic extract for 28 days significantly (p < 0.05) ameliorated the consequences of ovariectomy induced bone porosity and restored the normal architecture of bone, as compared to OVX control. The phytochemical screening of both the extracts were also carried out. The quantification of phytoconstituents showed the presence of β-sitosterol and lupeol in petroleum ether extract, whereas the lupeol is also quantified in the methanolic extract. The presence of gallic acid was quantified in methanolic extract using HPLC., Conclusion: B. ceiba: stem bark ameliorated the state of bone fragility and fracture possibly due to estrogenic modulation, as also confirmed by in-vitro osteogenic activity which may be due to the presence of lupeol, gallic acid and β-sitosterol constituents of the plant.
- Published
- 2018
- Full Text
- View/download PDF
13. Mechanism of Wound-Healing Activity of Hippophae rhamnoides L. Leaf Extract in Experimental Burns.
- Author
-
Upadhyay NK, Kumar R, Siddiqui MS, and Gupta A
- Abstract
The present investigation was undertaken to evaluate the healing efficacy of lyophilized aqueous leaf extract of Sea buckthorn (Hippophae rhamnoides L., family Elaeagnaceae) (SBT) and to explore its possible mechanism of action on experimental burn wounds in rats. The SBT extract, at various concentrations, was applied topically, twice daily for 7 days. Treatment with silver sulfadiazine (SSD) ointment was used as reference control. The most effective concentration of the extract was found to be 5.0% (w/w) for burn wound healing and this was further used for detailed study. The SBT-treated group showed faster reduction in wound area in comparison with control and SSD-treated groups. The topical application of SBT increased collagen synthesis and stabilization at the wound site, as evidenced by increase in hydroxyproline, hexosamine levels and up-regulated expression of collagen type-III. The histological examinations and matrix metalloproteinases (MMP-2 and -9) expression also confirmed the healing efficacy of SBT leaf extract. Furthermore, there was significant increase in levels of endogenous enzymatic and non-enzymatic antioxidants and decrease in lipid peroxide levels in SBT-treated burn wound granulation tissue. The SBT also promoted angiogenesis as evidenced by an in vitro chick chorioallantoic membrane model and in vivo up-regulated vascular endothelial growth factor (VEGF) expression. The SBT leaf extract had no cytotoxic effect on BHK-21 cell line. In conclusion, SBT aqueous leaf extract possesses significant healing potential in burn wounds and has a positive influence on the different phases of wound repair.
- Published
- 2011
- Full Text
- View/download PDF
14. Antioxidant, cytoprotective and antibacterial effects of Sea buckthorn (Hippophae rhamnoides L.) leaves.
- Author
-
Upadhyay NK, Kumar MS, and Gupta A
- Subjects
- Animals, Bacteria drug effects, Benzothiazoles chemistry, Biphenyl Compounds chemistry, Cell Line, Chromatography, High Pressure Liquid, Cricetinae, Ethanol, Flavonoids analysis, Microbial Sensitivity Tests, Oxidants chemistry, Phenols analysis, Picrates chemistry, Plant Extracts chemistry, Plant Extracts pharmacology, Plant Leaves chemistry, Reducing Agents chemistry, Solvents, Sulfonic Acids chemistry, Water, Anti-Bacterial Agents pharmacology, Antioxidants pharmacology, Cell Survival drug effects, Hippophae chemistry
- Abstract
The present study was carried out to investigate the antioxidant, cytoprotective and antibacterial effects of aqueous and hydroalcoholic extracts of Hippophae rhamnoides L. (Sea buckthorn) (SBT) leaves by using various in vitro systems and analysis of marker compounds by reverse phase-high performance liquid chromatography (RP-HPLC). The chemical composition of the leaf extracts was quantified by colorimetric reaction in terms of total phenol and flavonoids contents. Further, some of its bioactive phenolic constituents, such as quercetin-3-O-galactoside, quercetin-3-O-glucoside, kaempferol and isorhamnetin were also quantified in both SBT leaf extracts by RP-HPLC. The SBT leaf extracts exhibited potent antioxidant activity determined by 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 2,2-diphenyl-1-picryl-hydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays. Further, both extracts were observed to have cytoprotective activity against hydrogen peroxide and hypoxanthine-xanthine oxidase induced damage to BHK-21 cell line. The SBT leaf extracts showed growth inhibiting effect against Bacillus cereus, Pseudomonas aeruginosa, Staphylococcus aureus and Enterococcus faecalis. These observations suggest that aqueous and hydroalcoholic extracts of Sea buckthorn leaves have marked antioxidant, cytoprotective and antibacterial activities., (Copyright © 2010 Elsevier Ltd. All rights reserved.)
- Published
- 2010
- Full Text
- View/download PDF
15. Safety and healing efficacy of Sea buckthorn (Hippophae rhamnoides L.) seed oil on burn wounds in rats.
- Author
-
Upadhyay NK, Kumar R, Mandotra SK, Meena RN, Siddiqui MS, Sawhney RC, and Gupta A
- Subjects
- Animals, Anti-Infective Agents, Local therapeutic use, Blood Cell Count, Blood Chemical Analysis, Blotting, Western, Body Weight drug effects, Burns pathology, Carbon Dioxide, Chromatography, Supercritical Fluid, Electrophoresis, Polyacrylamide Gel, Glutathione metabolism, Irritants toxicity, Male, Matrix Metalloproteinases metabolism, Organ Size drug effects, Rats, Rats, Sprague-Dawley, Reactive Oxygen Species analysis, Seeds chemistry, Silver Sulfadiazine therapeutic use, Burns drug therapy, Hippophae chemistry, Plant Oils therapeutic use, Plant Oils toxicity
- Abstract
The present investigation was undertaken to determine the safety and efficacy of supercritical CO2-extracted Hippophae rhamnoides L. (Sea buckthorn) (SBT) seed oil on burn wound model. SBT seed oil was co-administered by two routes at a dose of 2.5 ml/kg body weight (p.o.) and 200 microl (topical) for 7 days on experimental burn wounds in rats. The SBT seed oil augmented the wound healing process as indicated by significant increase in wound contraction, hydroxyproline, hexosamine, DNA and total protein contents in comparison to control and reference control treated with silver sulfadiazine (SS) ointment. Histopathological findings further confirmed the healing potential of SBT seed oil. SBT seed oil treatment up-regulated the expression of matrix metalloproteinases (MMP-2 and 9), collagen type-III and VEGF in granulation tissue. It was observed that SBT seed oil also possesses antioxidant properties as evidenced by significant increase in reduced glutathione (GSH) level and reduced production of reactive oxygen species (ROS) in wound granulation tissue. In acute and sub-acute oral toxicity studies, no adverse effects were observed in any of the groups administered with SBT seed oil. These results suggest that the supercritical CO2-extracted Sea buckthorn seed oil possesses significant wound healing activity and have no associated toxicity or side effects.
- Published
- 2009
- Full Text
- View/download PDF
16. Effects of Rhodiola imbricata on dermal wound healing.
- Author
-
Gupta A, Kumar R, Upadhyay NK, Pal K, Kumar R, and Sawhney RC
- Subjects
- Animals, Male, Plant Extracts administration & dosage, Plant Extracts therapeutic use, Rats, Rats, Sprague-Dawley, Rhizome, Skin pathology, Phytotherapy, Plant Extracts pharmacology, Rhodiola, Skin injuries, Wound Healing drug effects
- Abstract
Rhodiola imbricata (Crassulaceae) is a perennial herb growing at high-altitude (4000 - 5000 m) in the western Himalayas. The present study was aimed at investigating the healing efficacy of R. imbricata rhizome ethanol extract using a rat excision wound model. The extract was found to be rich in polyphenols. The extract treated wounds were found to heal much faster as indicated by the improved rate of wound contraction and decreased time taken for epithelialization (11.33 vs. 18.33 days in the untreated group). The extract treatment increased cellular proliferation and collagen synthesis at the wound site, as evidenced by the increases in DNA, protein, hydroxyproline and hexosamine contents in comparison to a positive control treated with povidone-iodine ointment. These results were also supported by histological examinations. Furthermore, the treatment also caused an increase in antioxidant and a decrease in lipid peroxide levels in the granulation tissue. The results suggest that R. imbricata rhizome ethanol extract possesses a significant wound healing activity.
- Published
- 2007
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.