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1. Comparative genomics reveals high biological diversity and specific adaptations in the industrially and medically important fungal genus Aspergillus

Author

de Vries, Ronald P, Riley, Robert, Wiebenga, Ad, Aguilar-Osorio, Guillermo, Amillis, Sotiris, Uchima, Cristiane Akemi, Anderluh, Gregor, Asadollahi, Mojtaba, Askin, Marion, Barry, Kerrie, Battaglia, Evy, Bayram, Özgür, Benocci, Tiziano, Braus-Stromeyer, Susanna A, Caldana, Camila, Cánovas, David, Cerqueira, Gustavo C, Chen, Fusheng, Chen, Wanping, Choi, Cindy, Clum, Alicia, dos Santos, Renato Augusto Corrêa, Damásio, André Ricardo de Lima, Diallinas, George, Emri, Tamás, Fekete, Erzsébet, Flipphi, Michel, Freyberg, Susanne, Gallo, Antonia, Gournas, Christos, Habgood, Rob, Hainaut, Matthieu, Harispe, María Laura, Henrissat, Bernard, Hildén, Kristiina S, Hope, Ryan, Hossain, Abeer, Karabika, Eugenia, Karaffa, Levente, Karányi, Zsolt, Kraševec, Nada, Kuo, Alan, Kusch, Harald, LaButti, Kurt, Lagendijk, Ellen L, Lapidus, Alla, Levasseur, Anthony, Lindquist, Erika, Lipzen, Anna, Logrieco, Antonio F, MacCabe, Andrew, Mäkelä, Miia R, Malavazi, Iran, Melin, Petter, Meyer, Vera, Mielnichuk, Natalia, Miskei, Márton, Molnár, Ákos P, Mulé, Giuseppina, Ngan, Chew Yee, Orejas, Margarita, Orosz, Erzsébet, Ouedraogo, Jean Paul, Overkamp, Karin M, Park, Hee-Soo, Perrone, Giancarlo, Piumi, Francois, Punt, Peter J, Ram, Arthur FJ, Ramón, Ana, Rauscher, Stefan, Record, Eric, Riaño-Pachón, Diego Mauricio, Robert, Vincent, Röhrig, Julian, Ruller, Roberto, Salamov, Asaf, Salih, Nadhira S, Samson, Rob A, Sándor, Erzsébet, Sanguinetti, Manuel, Schütze, Tabea, Sepčić, Kristina, Shelest, Ekaterina, Sherlock, Gavin, Sophianopoulou, Vicky, Squina, Fabio M, Sun, Hui, Susca, Antonia, Todd, Richard B, Tsang, Adrian, Unkles, Shiela E, van de Wiele, Nathalie, van Rossen-Uffink, Diana, Oliveira, Juliana Velasco de Castro, Vesth, Tammi C, Visser, Jaap, Yu, Jae-Hyuk, Zhou, Miaomiao, and Andersen, Mikael R

Subjects
Human Genome, Genetics, Biotechnology, Emerging Infectious Diseases, Infectious Diseases, Adaptation, Biological, Aspergillus, Biodiversity, Biomass, Carbon, Computational Biology, Cytochrome P-450 Enzyme System, DNA Methylation, Fungal Proteins, Gene Expression Regulation, Fungal, Gene Regulatory Networks, Genome, Fungal, Genomics, Humans, Metabolic Networks and Pathways, Molecular Sequence Annotation, Multigene Family, Oxidoreductases, Phylogeny, Plants, Secondary Metabolism, Signal Transduction, Stress, Physiological, Genome sequencing, Comparative genomics, Fungal biology, Environmental Sciences, Biological Sciences, Information and Computing Sciences, Bioinformatics
Abstract

BackgroundThe fungal genus Aspergillus is of critical importance to humankind. Species include those with industrial applications, important pathogens of humans, animals and crops, a source of potent carcinogenic contaminants of food, and an important genetic model. The genome sequences of eight aspergilli have already been explored to investigate aspects of fungal biology, raising questions about evolution and specialization within this genus.ResultsWe have generated genome sequences for ten novel, highly diverse Aspergillus species and compared these in detail to sister and more distant genera. Comparative studies of key aspects of fungal biology, including primary and secondary metabolism, stress response, biomass degradation, and signal transduction, revealed both conservation and diversity among the species. Observed genomic differences were validated with experimental studies. This revealed several highlights, such as the potential for sex in asexual species, organic acid production genes being a key feature of black aspergilli, alternative approaches for degrading plant biomass, and indications for the genetic basis of stress response. A genome-wide phylogenetic analysis demonstrated in detail the relationship of the newly genome sequenced species with other aspergilli.ConclusionsMany aspects of biological differences between fungal species cannot be explained by current knowledge obtained from genome sequences. The comparative genomics and experimental study, presented here, allows for the first time a genus-wide view of the biological diversity of the aspergilli and in many, but not all, cases linked genome differences to phenotype. Insights gained could be exploited for biotechnological and medical applications of fungi.

Published
2017

7. Determination of the essentiality of the eight cysteine residues of the NrtA protein for high-affinity nitrate transport and the generation of a functional cysteine-less transporter

Author

Unkles, Shiela E., Stephenson, Raymond M., Rouch, Duncan A., Kinghorn, James R., Ye Wang, Glass, Anthony D.M., Siddiqi, M. Yaeesh, and Okamoto, Mamoru

Subjects
Aspergillus -- Research, Membrane proteins -- Research, Cysteine -- Research, Biological sciences, Chemistry
Abstract

The eight natural cysteine residues present in the putative transmembrane domains (Tm) of the NrtA high-affinity nitrate transporter protein from the lower eukaryote Aspergillus nidulans were altered using site-specific mutagenesis. The essentiality of each of the cysteine residues was determined.

Published
2005

8. Missense mutations that inactivate the Aspergillus nidulans nrtA gene encoding a high-affinity nitrate transporter

Author

Kinghorn, James R., Sloan, Joan, Kana'n, Ghassan J.M., DaSilva, Edisio R., Rouch, Duncan A., and Unkles, Shiela E.

Subjects
Genetic code -- Research, Aspergillus -- Genetic aspects, Gene mutations -- Research, Genetic research, Biological sciences
Abstract

The transport of nitrate into prokaryotic and eukaryotic cells, of considerable interest to agriculture, ecology, and human health, is carried out by members of a distinct cluster of proteins within the major facilitator superfamily. To obtain structure/function information on this important class of nitrate permeases, a collection of chemically induced mutations in the nrtA gene encoding a 12-transmembrane domain, high-affinity nitrate transporter from the eukaryote Aspergillus nidulans was isolated and characterized. This mutational analysis, coupled with protein alignments, demonstrates the utility of the approach to predicting peptide motifs and individual residues important for the movement of nitrate across the membrane. These include the highly conserved nitrate signature motif (residues 166-173) in Tm 5, the conserved charged residues Arg87 (Tm 2) and Arg368 (Tm 8), as well as the aromatic residue Phe47 (Tm 1), all within transmembrane helices. No mutations were observed in the large central loop (Lp 6/7) between Tm 6 and Tm 7. Finally, the study of a strain with a conversion of Trp481 (Tm 12) to a stop codon suggests that all 12 transmembrane domains and/or the C-terminal tail are required for membrane insertion and/or stability of NrtA.

Published
2005

11. Mutational analysis of the gephyrin-related molybdenum cofactor biosynthetic gene cnxE from the lower eukaryote Aspergillus nidulans

Author

Heck, Immanuel S., Schrag, Joseph D., Sloan, Joan, Millar, Lindsey J., Kanan, Ghassa, Kinghorn, James R., and Unkles, Shiela E.

Subjects
Genetic research -- Analysis, Amino acids -- Genetic aspects, Mutation (Biology) -- Research, Biological sciences
Abstract

We report the identification of a number of mutations that result in amino acid replacements (and their phenotypic characterization) in either the MogA-like domain or domains 2 and 3 of the MoeA-like region of the Aspergillus nidulans cnx[EPSILON] gene. These domains are functionally required since mutations that result in amino acid substitutions in any one domain lead to the loss or to a substantial reduction in all three identified molybdoenzyme activities (i.e., nitrate reductase, xanthine dehydrogenase, and nicotinate hydroxylase). Certain cnx[EPSILON] mutants that show partial growth with nitrate as the nitrogen source in contrast do not grow on hypoxanthine or nicotinate. Complementation between mutants carrying lesions in the MogA-like domain or the MoeA-like region, respectively, most likely occurs at the protein level. A homology model of Cnx[EPSILON] based on the dimeric structure of [EPSILON]. coli MoeA is presented and the position of inactivating mutations (due to amino acid replacements) in the MoeA-like functional region of the Cnx[EPSILON] protein is mapped to this model. Finally, the activity of nicotinate hydroxylase, unlike that of nitrate reductase and xanthine dehydrogenase, is not restored in cnx[EPSILON] mutants grown in the presence of excess molybdate.

Published
2002

12. Genetic aspects of antibiotic resistance, haemolysin and bacteriocin production in enterococci

Author

Unkles, Shiela E. and Thirkell, David

Subjects
572.8, QR82.E6U6, Enterobacteriaceae
Abstract

A previous survey of enterococci had identified five strains of Streptococcus faecalis (K55 and SB94) - two subspecies liquefaciens (K60 and K88) and one zymogenes (K87) - and two S. faecium strains (K46 and SB69) which were resistant to tetracycline and streptomycin but susceptible to gentamicin. All the S. faecalis strains and K46 were in addition resistant to erythromycin but only the S. faecium strains were penicillin and ampicillin resistant. The minimal inhibitory concentrations of a further six antibiotics were determined. These values confirmed that in S. faecalis strains, erythromycin resistance was accompanied by resistance to lincomycin and pristinamycin IA, a phenotype typical of macrolide - lincosamide - streptogramin B - type (MLS) antibiotics resistance. The erythromycin resistant K46 however, although resistant to lincomycin, was pristinamycin susceptible and so the basis of resistance is unknown. S. faecalis K60, K87 and SB94 were resistant to kanamycin and neomycin as was S. faecium K46 but all strains were susceptible to spectinomycin. The phenotypes were consistent with resistance mediated by enzymic modification of streptomycin with adenyltransferase (6) and of kanamycin and neomycin with phosphotransferase (3') (5")-III. Erythromycin and tetracycline resistances were expressed constitutively in all strains. Only one S. faecalis (K88) was found to be chloramphenicol resistant and as is typical of Gram-positive bacteria, resistance was inducible. The ability to produce bacteriocin was restricted to beta-haemolytic strain K87 and to strain SB94. Subsequent results indicated that strain K87 probably produced more than one bacteriocin, the activity of which was repressed in the parental strain but which, in derivatives, could be enhanced by the presence of streptomycin. Evidence for the location of resistance, haemolysin and bacteriocin genes was sought from study of the transfer characteristics and stability of markers and from examination of the plasmid content of parental strains and their derivatives. The well characterised S. faecalis subspecies zymogenes strain DS5 (Clewell et al., 1982b) was included for comparison in transfer and curing experiments. All the S. faecalis strains aggregated in response to a cell free filtrate of a plasmid free recipient strain JH2-1, indicating the presence of at least one conjugative plasmid although the low transfer frequencies of most resistance genes in broth matings suggested that response was not necessarily encoded by antibiotic resistance plasmids. Transfer of beta-haemolytic activity and all resistance markers was observed after broth matings but the range of transfer frequencies between strains was wide. Furthermore, the incidence of transfer could be variable particularly in the transfer of DS5 erythromycin resistance and all K87 antibiotic resistances which seemed to be dependent on the production of active donor bacteriocin. Matings of S. faecalis strains carried out on membrane filters were only marginally more efficient in terms of transfer frequencies but were superior with regard to reproducibility of transfer. No antibiotic resistance transfer from S. faecium donors was observed after broth matings and only SB69 tetracycline resistance transferred after filter mating at very low frequency. Several resistance determinants and those encoding β-haemolysin were found to be capable of retransfer indicative of association with genes specifying conjugative ability. Analysis of transconjugant phenotypes revealed that the tetracycline resistance gene of K55, the streptomycin resistance gene of K88 and β-haemolytic activities were always transferred alone but some resistance markers were usually co-transferred with other donor markers.

Published
1986

14. Heterologous expression of a basic elicitin from Phytophthora cryptogea in Phytophthora infestans increases its ability to cause leaf necrosis in tobacco

Author

Panabieres, Franck, Birch, Paul R.J., Unkles, Shiela E., Ponchet, Michel, Lacourt, Isabelle, Venard, Paul, Keller, Harald, Allasia, Valerie, Ricci, Pierre, and Duncan, James M.

Subjects
Plant-pathogen relationships -- Genetic aspects, Bacteria, Phytopathogenic -- Genetic aspects, Tobacco -- Diseases and pests, Biological sciences
Abstract

A study was undertaken showing the transcription and translation of a gene encoding a basic elicitin, cryptogein, derived from the Phytophthora cryptogea in Phytophthora infestans. The genus Phytophthora is composed of a diverse group of plant pathogenic organisms that mostly produced diseases in crops such as tobacco. Results revealed that cryptogein b produced from P. infestans co-transformants increased the generation of hypersensitive-like necrosis of tobacco leaves. The transposition of a gene encoding a basic elicitin from one species to another altered the phenotypic interaction.

Published
1998

16. The tigA gene is a transcriptional fusion of glycolytic genes encoding triose-phosphate isomerase and glyceraldehyde-3-phosphate dehydrogenase in Oomycota

Author

Unkles, Shiela E., Logsdon, John M., Jr., Robison, Keith, Kinghorn, James R., and Duncan, James M.

Subjects
Genetic transcription -- Research, Isomerases -- Research, Dehydrogenases -- Research, Eukaryotic cells -- Research, Biological sciences
Abstract

A study was conducted on a fusion between the tpi and gpd genes encoding TPI and GAPDH, a gene arrangement which seems to be prevalent throughout the phylum Oomycota. Southern and PCR analyses indicate a similar organization that exists in other closely related genera such as Pythium. Moreover, TPI may not have a significant role in resolving relationships among major eukaryotic groups.

Published
1997

17. The Gibberella fujikuroi niaD gene encoding nitrate reductase: isolation, sequence, homologous transformation and electrophoretic karyotype location

Author

Tudzynski, Bettina, Mende, Katrin, Weltring, Klaus-Michael, Kinghorn, James R., and Unkles, Shiela E.

Subjects
Fungi -- Genetic aspects, Karyotypes -- Research, Biological sciences
Abstract

An efficient homologous transformation system has been developed with the use of the niaD gene, which encodes for nitrate reductase in Gibberella fujikuroi. The electrophoretic karyotype location and basic gene organization of Gibberella fujikuroi is also determined. The system is shown to be useful for self-cloning of gibberellin biosynthetic genes and gene disruption experiments.

Published
1996

19. High-affinity nitrate/nitrite transporters NrtA and NrtB of Aspergillus nidulans exhibit high specificity and different inhibitor sensitivity

Author

Akhtar, Naureen, Karabika, Eugenia, Kinghorn, James R, Glass, Anthony D M, Unkles, Shiela E, Rouch, Duncan A, BBSRC, University of St Andrews. School of Biology, and University of St Andrews. Biomedical Sciences Research Complex

Subjects
Antifungal Agents, Nitrates, Inhibitors, Nitrogen, QH301 Biology, Physiology and Metabolism, Anion Transport Proteins, Cesium, Microbial Sensitivity Tests, Kinetic analysis, Standard, Aspergillus nidulans, Culture Media, Substrate Specificity, Fungal Proteins, QH301, Aspergillus, Chlorides, Chlorates, Sulfites, Nitrate transport, Nitrites
Abstract

The NrtA and NrtB nitrate transporters are paralogous members of the major facilitator superfamily in Aspergillus nidulans. The availability of loss-of-function mutations allowed individual investigation of the specificity and inhibitor sensitivity of both NrtA and NrtB. In this study, growth response tests were carried out at a growth limiting concentration of nitrate (1 mM) as the sole nitrogen source, in the presence of a number of potential nitrate analogues at various concentrations, to evaluate their effect on nitrate transport. Both chlorate and chlorite inhibited fungal growth, with chlorite exerting the greater inhibition. The main transporter of nitrate, NrtA, proved to be more sensitive to chlorate than the minor transporter, NrtB. Similarly, the cation caesium was shown to exert differential effects, strongly inhibiting the activity of NrtB, but not NrtA. In contrast, no inhibition of nitrate uptake by NrtA or NrtB transporters was observed in either growth tests or uptake assays in the presence of bicarbonate, formate, malonate, or oxalate (sulphite could not be tested in uptake assays due to its reaction with nitrate), indicating significant specificity of nitrate transport. Kinetic analyses of nitrate uptake revealed that both chlorate and chlorite inhibited NrtA competitively, while these same inhibitors inhibited NrtB in a non-competitive fashion. The caesium ion appeared to inhibit NrtA in a non-competitive fashion, while NrtB was inhibited uncompetitively. The results provide further evidence of the distinctly different characteristics as well as the high specificity of nitrate uptake by these two transporters. Publisher PDF

Published
2015

22. Comparative genomics reveals high biological diversity and specific adaptations in the industrially and medically important fungal genus Aspergillus

Author

Vries, Ronald P. de, Riley, Robert, Wiebenga, Ad, Aguilar-Osorio, Guillermo, Amillis, Sotiris, Akemi Uchima, Cristiane, Anderluh, Gregor, Asaollahi, Mojtaba, Askin, Marion, Barry, Kerrie, Battaglia, Evy, Bayram, Ozgur, Benocci, Tiziano, Braus-Stromeyer, Susanna A., Caldana, Camila, Cerqueira, Gustavo C., Chen, Fusheng, Chen, Wanping, Choi, Cindy, Clum, Alicia, Diallinas, George, Flipphi, Michel, Freyburg, Susanne, Gallo, Antonia, Gournas, Christos, Habgood, Rob, Hainaut, Matthieu, Harispe, Maria Laura, Henrissat, Bernard, Hope, Ryan, Hossain, Abeer, Karabika, Eugenia, Karaffa, Levente, Karanyi, Zsolt, Krasevec, Nada, Kuo, Alan, Kusch, Harald, LaButti, Kurt, Lagendijk, Ellen L., Lapidus, Alla, Levasseur, Anthony, Lindquist, Erika, Lipzen, Anna, Logrieco, Antonio F., MacCabe, Andrew, Malavazi, Iran, Melin, Petter, Meyer, Vera, Mielnichuk, Natalia, Ngan, Chew Yee, Orejas, Margarita, Ouedraogo, Jean Paul, Overkamp, Karin M., Park, Hee-Soo, Perrone, Giancarlo, Piumi, Francois, Punt, Peter J., Ram, Arthur F.J., Ramon, Ana, Rauscher, Stefan, Record, Eric, Robert, Vincent, Ruller, Roberto, Salamov, Asaf, Salih, Nadhira S., Samson, Rob A., Sanguinetti, Manuel, Sep?i?, Kristina, Shelest, Ekaterina, Sherlock, Gavin, Sophianopoulou, Vicky, Squina, Fabio M., Sun, Hui, Susca, Antonia, Todd, Richard B., Tsang, Adrian, Unkles, Shiela E., Wiele, Nathalie van de, Rossen-Uffink, Diana van, Velasco de Castro Oliveira, Juliana, Vesth, Tammi C., Visser, Jaap, Yu, Jae-Hyuk, Zhou, Miaomiao, Andersen, Mikael R., Archer, David B., Baker, Scott E., Benoit, Isabelle, Brakhage, Axel A., Braus, Gerhard H., Fischer, Reinhard, Frisvad, Jens C., Goldman, Gustavo H., Houbraken, Jos, Oakley, Berl, Scazzocchio, Claudio, Seiboth, Bernhard, vanKuyk, Patricia A., Wortman, Jennifer, Dyer, Paul S., and Grigoriev, Igor V.

Subjects
Genome sequencing, Comparative genomics, Fungal biology
Abstract

Background: The fungal genus Aspergillus is of critical importance to humankind. Species include those with industrial applications, important pathogens of humans, animals and crops, a source of potent carcinogenic contaminants of food, and an important genetic model. The genome sequences of eight aspergilli have already been explored to investigate aspects of fungal biology, raising questions about evolution and specialization within this genus. Results: We have generated genome sequences for ten novel, highly diverse Aspergillus species and compared these in detail to sister and more distant genera. Comparative studies of key aspects of fungal biology, including primary and secondary metabolism, stress response, biomass degradation, and signal transduction, revealed both conservation and diversity among the species. Observed genomic differences were validated with experimental studies. This revealed several highlights, such as the potential for sex in asexual species, organic acid production genes being a key feature of black aspergilli, alternative approaches for degrading plant biomass, and indications for the genetic basis of stress response. A genome-wide phylogenetic analysis demonstrated in detail the relationship of the newly genome sequenced species with other aspergilli. Conclusions: Many aspects of biological differences between fungal species cannot be explained by current knowledge obtained from genome sequences. The comparative genomics and experimental study, presented here, allows for the first time a genus-wide view of the biological diversity of the aspergilli and in many, but not all, cases linked genome differences to phenotype. Insights gained could be exploited for biotechnological and medical applications of fungi.

Published
2017

23. Additional file 8: of Comparative genomics reveals high biological diversity and specific adaptations in the industrially and medically important fungal genus Aspergillus

Author

Vries, Ronald De, Riley, Robert, Wiebenga, Ad, Aguilar-Osorio, Guillermo, Sotiris Amillis, Cristiane Uchima, Anderluh, Gregor, Asadollahi, Mojtaba, Askin, Marion, Barry, Kerrie, Battaglia, Evy, Bayram, Özgür, Benocci, Tiziano, Braus-Stromeyer, Susanna, Caldana, Camila, Cánovas, David, Cerqueira, Gustavo, Fusheng Chen, Wanping Chen, Choi, Cindy, Clum, Alicia, Santos, Renato Dos, Damásio, André, Diallinas, George, Emri, Tamás, Fekete, Erzsébet, Flipphi, Michel, Freyberg, Susanne, Gallo, Antonia, Gournas, Christos, Habgood, Rob, Hainaut, Matthieu, Harispe, María, Henrissat, Bernard, Hildén, Kristiina, Hope, Ryan, Hossain, Abeer, Karabika, Eugenia, Karaffa, Levente, Karányi, Zsolt, Kraševec, Nada, Kuo, Alan, Kusch, Harald, LaButti, Kurt, Lagendijk, Ellen, Lapidus, Alla, Levasseur, Anthony, Lindquist, Erika, Lipzen, Anna, Logrieco, Antonio, MacCabe, Andrew, Mäkelä, Miia, Malavazi, Iran, Melin, Petter, Meyer, Vera, Mielnichuk, Natalia, Miskei, Márton, Molnár, Ákos, Mulé, Giuseppina, Chew Ngan, Orejas, Margarita, Orosz, Erzsébet, Ouedraogo, Jean, Overkamp, Karin, Park, Hee-Soo, Perrone, Giancarlo, Francois Piumi, Punt, Peter, Ram, Arthur, Ramón, Ana, Rauscher, Stefan, Record, Eric, Riaño-Pachón, Diego, Robert, Vincent, Röhrig, Julian, Ruller, Roberto, Salamov, Asaf, Nadhira Salih, Samson, Rob, Sándor, Erzsébet, Sanguinetti, Manuel, Schütze, Tabea, Sepčić, Kristina, Shelest, Ekaterina, Sherlock, Gavin, Sophianopoulou, Vicky, Squina, Fabio, Sun, Hui, Susca, Antonia, Todd, Richard, Tsang, Adrian, Unkles, Shiela, Wiele, Nathalie Van De, Rossen-Uffink, Diana Van, Oliveira, Juliana, Vesth, Tammi, Visser, Jaap, Jae-Hyuk Yu, Miaomiao Zhou, Andersen, Mikael, Archer, David, Baker, Scott, Benoit, Isabelle, Brakhage, Axel, Braus, Gerhard, Fischer, Reinhard, Frisvad, Jens, Goldman, Gustavo, Houbraken, Jos, Berl Oakley, Pócsi, István, Scazzocchio, Claudio, Seiboth, Bernhard, VanKuyk, Patricia, Wortman, Jennifer, Dyer, Paul, and Grigoriev, Igor

Subjects
body regions, inorganic chemicals, nervous system, fungi
Abstract

Effect of SHAM and KCN on fungal growth and sporulation. (PDF 252 kb)

Published
2017
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24. Additional file 27: of Comparative genomics reveals high biological diversity and specific adaptations in the industrially and medically important fungal genus Aspergillus

Author

Vries, Ronald De, Riley, Robert, Wiebenga, Ad, Aguilar-Osorio, Guillermo, Sotiris Amillis, Cristiane Uchima, Anderluh, Gregor, Asadollahi, Mojtaba, Askin, Marion, Barry, Kerrie, Battaglia, Evy, Bayram, Özgür, Benocci, Tiziano, Braus-Stromeyer, Susanna, Caldana, Camila, Cánovas, David, Cerqueira, Gustavo, Fusheng Chen, Wanping Chen, Choi, Cindy, Clum, Alicia, Santos, Renato Dos, Damásio, André, Diallinas, George, Emri, Tamás, Fekete, Erzsébet, Flipphi, Michel, Freyberg, Susanne, Gallo, Antonia, Gournas, Christos, Habgood, Rob, Hainaut, Matthieu, Harispe, María, Henrissat, Bernard, Hildén, Kristiina, Hope, Ryan, Hossain, Abeer, Karabika, Eugenia, Karaffa, Levente, Karányi, Zsolt, Kraševec, Nada, Kuo, Alan, Kusch, Harald, LaButti, Kurt, Lagendijk, Ellen, Lapidus, Alla, Levasseur, Anthony, Lindquist, Erika, Lipzen, Anna, Logrieco, Antonio, MacCabe, Andrew, Mäkelä, Miia, Malavazi, Iran, Melin, Petter, Meyer, Vera, Mielnichuk, Natalia, Miskei, Márton, Molnár, Ákos, Mulé, Giuseppina, Chew Ngan, Orejas, Margarita, Orosz, Erzsébet, Ouedraogo, Jean, Overkamp, Karin, Park, Hee-Soo, Perrone, Giancarlo, Francois Piumi, Punt, Peter, Ram, Arthur, Ramón, Ana, Rauscher, Stefan, Record, Eric, Riaño-Pachón, Diego, Robert, Vincent, Röhrig, Julian, Ruller, Roberto, Salamov, Asaf, Nadhira Salih, Samson, Rob, Sándor, Erzsébet, Sanguinetti, Manuel, Schütze, Tabea, Sepčić, Kristina, Shelest, Ekaterina, Sherlock, Gavin, Sophianopoulou, Vicky, Squina, Fabio, Sun, Hui, Susca, Antonia, Todd, Richard, Tsang, Adrian, Unkles, Shiela, Wiele, Nathalie Van De, Rossen-Uffink, Diana Van, Oliveira, Juliana, Vesth, Tammi, Visser, Jaap, Jae-Hyuk Yu, Miaomiao Zhou, Andersen, Mikael, Archer, David, Baker, Scott, Benoit, Isabelle, Brakhage, Axel, Braus, Gerhard, Fischer, Reinhard, Frisvad, Jens, Goldman, Gustavo, Houbraken, Jos, Berl Oakley, Pócsi, István, Scazzocchio, Claudio, Seiboth, Bernhard, VanKuyk, Patricia, Wortman, Jennifer, Dyer, Paul, and Grigoriev, Igor

Abstract

Maximum likelihood rooted phylogeny of the FCY-like transporters of the aspergilli and some penicillia. (PDF 324 kb)

Published
2017
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25. Additional file 7: of Comparative genomics reveals high biological diversity and specific adaptations in the industrially and medically important fungal genus Aspergillus

Author

Vries, Ronald De, Riley, Robert, Wiebenga, Ad, Aguilar-Osorio, Guillermo, Sotiris Amillis, Cristiane Uchima, Anderluh, Gregor, Asadollahi, Mojtaba, Askin, Marion, Barry, Kerrie, Battaglia, Evy, Bayram, Özgür, Benocci, Tiziano, Braus-Stromeyer, Susanna, Caldana, Camila, Cánovas, David, Cerqueira, Gustavo, Fusheng Chen, Wanping Chen, Choi, Cindy, Clum, Alicia, Santos, Renato Dos, Damásio, André, Diallinas, George, Emri, Tamás, Fekete, Erzsébet, Flipphi, Michel, Freyberg, Susanne, Gallo, Antonia, Gournas, Christos, Habgood, Rob, Hainaut, Matthieu, Harispe, María, Henrissat, Bernard, Hildén, Kristiina, Hope, Ryan, Hossain, Abeer, Karabika, Eugenia, Karaffa, Levente, Karányi, Zsolt, Kraševec, Nada, Kuo, Alan, Kusch, Harald, LaButti, Kurt, Lagendijk, Ellen, Lapidus, Alla, Levasseur, Anthony, Lindquist, Erika, Lipzen, Anna, Logrieco, Antonio, MacCabe, Andrew, Mäkelä, Miia, Malavazi, Iran, Melin, Petter, Meyer, Vera, Mielnichuk, Natalia, Miskei, Márton, Molnár, Ákos, Mulé, Giuseppina, Chew Ngan, Orejas, Margarita, Orosz, Erzsébet, Ouedraogo, Jean, Overkamp, Karin, Park, Hee-Soo, Perrone, Giancarlo, Francois Piumi, Punt, Peter, Ram, Arthur, Ramón, Ana, Rauscher, Stefan, Record, Eric, Riaño-Pachón, Diego, Robert, Vincent, Röhrig, Julian, Ruller, Roberto, Salamov, Asaf, Nadhira Salih, Samson, Rob, Sándor, Erzsébet, Sanguinetti, Manuel, Schütze, Tabea, Sepčić, Kristina, Shelest, Ekaterina, Sherlock, Gavin, Sophianopoulou, Vicky, Squina, Fabio, Sun, Hui, Susca, Antonia, Todd, Richard, Tsang, Adrian, Unkles, Shiela, Wiele, Nathalie Van De, Rossen-Uffink, Diana Van, Oliveira, Juliana, Vesth, Tammi, Visser, Jaap, Jae-Hyuk Yu, Miaomiao Zhou, Andersen, Mikael, Archer, David, Baker, Scott, Benoit, Isabelle, Brakhage, Axel, Braus, Gerhard, Fischer, Reinhard, Frisvad, Jens, Goldman, Gustavo, Houbraken, Jos, Berl Oakley, Pócsi, István, Scazzocchio, Claudio, Seiboth, Bernhard, VanKuyk, Patricia, Wortman, Jennifer, Dyer, Paul, and Grigoriev, Igor

Abstract

Comparative growth profiling of the aspergilli and selected other fungi. (PDF 8.65 kb)

Published
2017
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26. Additional file 6: of Comparative genomics reveals high biological diversity and specific adaptations in the industrially and medically important fungal genus Aspergillus

Author

Vries, Ronald De, Riley, Robert, Wiebenga, Ad, Aguilar-Osorio, Guillermo, Sotiris Amillis, Cristiane Uchima, Anderluh, Gregor, Asadollahi, Mojtaba, Askin, Marion, Barry, Kerrie, Battaglia, Evy, Bayram, Özgür, Benocci, Tiziano, Braus-Stromeyer, Susanna, Caldana, Camila, Cánovas, David, Cerqueira, Gustavo, Fusheng Chen, Wanping Chen, Choi, Cindy, Clum, Alicia, Santos, Renato Dos, Damásio, André, Diallinas, George, Emri, Tamás, Fekete, Erzsébet, Flipphi, Michel, Freyberg, Susanne, Gallo, Antonia, Gournas, Christos, Habgood, Rob, Hainaut, Matthieu, Harispe, María, Henrissat, Bernard, Hildén, Kristiina, Hope, Ryan, Hossain, Abeer, Karabika, Eugenia, Karaffa, Levente, Karányi, Zsolt, Kraševec, Nada, Kuo, Alan, Kusch, Harald, LaButti, Kurt, Lagendijk, Ellen, Lapidus, Alla, Levasseur, Anthony, Lindquist, Erika, Lipzen, Anna, Logrieco, Antonio, MacCabe, Andrew, Mäkelä, Miia, Malavazi, Iran, Melin, Petter, Meyer, Vera, Mielnichuk, Natalia, Miskei, Márton, Molnár, Ákos, Mulé, Giuseppina, Chew Ngan, Orejas, Margarita, Orosz, Erzsébet, Ouedraogo, Jean, Overkamp, Karin, Park, Hee-Soo, Perrone, Giancarlo, Francois Piumi, Punt, Peter, Ram, Arthur, Ramón, Ana, Rauscher, Stefan, Record, Eric, Riaño-Pachón, Diego, Robert, Vincent, Röhrig, Julian, Ruller, Roberto, Salamov, Asaf, Nadhira Salih, Samson, Rob, Sándor, Erzsébet, Sanguinetti, Manuel, Schütze, Tabea, Sepčić, Kristina, Shelest, Ekaterina, Sherlock, Gavin, Sophianopoulou, Vicky, Squina, Fabio, Sun, Hui, Susca, Antonia, Todd, Richard, Tsang, Adrian, Unkles, Shiela, Wiele, Nathalie Van De, Rossen-Uffink, Diana Van, Oliveira, Juliana, Vesth, Tammi, Visser, Jaap, Jae-Hyuk Yu, Miaomiao Zhou, Andersen, Mikael, Archer, David, Baker, Scott, Benoit, Isabelle, Brakhage, Axel, Braus, Gerhard, Fischer, Reinhard, Frisvad, Jens, Goldman, Gustavo, Houbraken, Jos, Berl Oakley, Pócsi, István, Scazzocchio, Claudio, Seiboth, Bernhard, VanKuyk, Patricia, Wortman, Jennifer, Dyer, Paul, and Grigoriev, Igor

Subjects
body regions, animal structures, nervous system, fungi
Abstract

Overview of number of putative orthologs/paralogs in the compared fungal species related to primary carbon metabolism. (PDF 237 kb)

Published
2017
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27. Additional file 5: of Comparative genomics reveals high biological diversity and specific adaptations in the industrially and medically important fungal genus Aspergillus

Author

Vries, Ronald De, Riley, Robert, Wiebenga, Ad, Aguilar-Osorio, Guillermo, Sotiris Amillis, Cristiane Uchima, Anderluh, Gregor, Asadollahi, Mojtaba, Askin, Marion, Barry, Kerrie, Battaglia, Evy, Bayram, Özgür, Benocci, Tiziano, Braus-Stromeyer, Susanna, Caldana, Camila, Cánovas, David, Cerqueira, Gustavo, Fusheng Chen, Wanping Chen, Choi, Cindy, Clum, Alicia, Santos, Renato Dos, Damásio, André, Diallinas, George, Emri, Tamás, Fekete, Erzsébet, Flipphi, Michel, Freyberg, Susanne, Gallo, Antonia, Gournas, Christos, Habgood, Rob, Hainaut, Matthieu, Harispe, María, Henrissat, Bernard, Hildén, Kristiina, Hope, Ryan, Hossain, Abeer, Karabika, Eugenia, Karaffa, Levente, Karányi, Zsolt, Kraševec, Nada, Kuo, Alan, Kusch, Harald, LaButti, Kurt, Lagendijk, Ellen, Lapidus, Alla, Levasseur, Anthony, Lindquist, Erika, Lipzen, Anna, Logrieco, Antonio, MacCabe, Andrew, Mäkelä, Miia, Malavazi, Iran, Melin, Petter, Meyer, Vera, Mielnichuk, Natalia, Miskei, Márton, Molnár, Ákos, Mulé, Giuseppina, Chew Ngan, Orejas, Margarita, Orosz, Erzsébet, Ouedraogo, Jean, Overkamp, Karin, Park, Hee-Soo, Perrone, Giancarlo, Francois Piumi, Punt, Peter, Ram, Arthur, Ramón, Ana, Rauscher, Stefan, Record, Eric, Riaño-Pachón, Diego, Robert, Vincent, Röhrig, Julian, Ruller, Roberto, Salamov, Asaf, Nadhira Salih, Samson, Rob, Sándor, Erzsébet, Sanguinetti, Manuel, Schütze, Tabea, Sepčić, Kristina, Shelest, Ekaterina, Sherlock, Gavin, Sophianopoulou, Vicky, Squina, Fabio, Sun, Hui, Susca, Antonia, Todd, Richard, Tsang, Adrian, Unkles, Shiela, Wiele, Nathalie Van De, Rossen-Uffink, Diana Van, Oliveira, Juliana, Vesth, Tammi, Visser, Jaap, Jae-Hyuk Yu, Miaomiao Zhou, Andersen, Mikael, Archer, David, Baker, Scott, Benoit, Isabelle, Brakhage, Axel, Braus, Gerhard, Fischer, Reinhard, Frisvad, Jens, Goldman, Gustavo, Houbraken, Jos, Berl Oakley, Pócsi, István, Scazzocchio, Claudio, Seiboth, Bernhard, VanKuyk, Patricia, Wortman, Jennifer, Dyer, Paul, and Grigoriev, Igor

Subjects
body regions, nervous system, fungi, behavior and behavior mechanisms, reproductive and urinary physiology
Abstract

Expression of mating and pheromone-signalling pathway genes in representative asexual aspergilli. (PDF 488 kb)

Published
2017
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28. Additional file 15: of Comparative genomics reveals high biological diversity and specific adaptations in the industrially and medically important fungal genus Aspergillus

Author

Vries, Ronald De, Riley, Robert, Wiebenga, Ad, Aguilar-Osorio, Guillermo, Sotiris Amillis, Cristiane Uchima, Anderluh, Gregor, Asadollahi, Mojtaba, Askin, Marion, Barry, Kerrie, Battaglia, Evy, Bayram, Özgür, Benocci, Tiziano, Braus-Stromeyer, Susanna, Caldana, Camila, Cánovas, David, Cerqueira, Gustavo, Fusheng Chen, Wanping Chen, Choi, Cindy, Clum, Alicia, Santos, Renato Dos, Damásio, André, Diallinas, George, Emri, Tamás, Fekete, Erzsébet, Flipphi, Michel, Freyberg, Susanne, Gallo, Antonia, Gournas, Christos, Habgood, Rob, Hainaut, Matthieu, Harispe, María, Henrissat, Bernard, Hildén, Kristiina, Hope, Ryan, Hossain, Abeer, Karabika, Eugenia, Karaffa, Levente, Karányi, Zsolt, Kraševec, Nada, Kuo, Alan, Kusch, Harald, LaButti, Kurt, Lagendijk, Ellen, Lapidus, Alla, Levasseur, Anthony, Lindquist, Erika, Lipzen, Anna, Logrieco, Antonio, MacCabe, Andrew, Mäkelä, Miia, Malavazi, Iran, Melin, Petter, Meyer, Vera, Mielnichuk, Natalia, Miskei, Márton, Molnár, Ákos, Mulé, Giuseppina, Chew Ngan, Orejas, Margarita, Orosz, Erzsébet, Ouedraogo, Jean, Overkamp, Karin, Park, Hee-Soo, Perrone, Giancarlo, Francois Piumi, Punt, Peter, Ram, Arthur, Ramón, Ana, Rauscher, Stefan, Record, Eric, Riaño-Pachón, Diego, Robert, Vincent, Röhrig, Julian, Ruller, Roberto, Salamov, Asaf, Nadhira Salih, Samson, Rob, Sándor, Erzsébet, Sanguinetti, Manuel, Schütze, Tabea, Sepčić, Kristina, Shelest, Ekaterina, Sherlock, Gavin, Sophianopoulou, Vicky, Squina, Fabio, Sun, Hui, Susca, Antonia, Todd, Richard, Tsang, Adrian, Unkles, Shiela, Wiele, Nathalie Van De, Rossen-Uffink, Diana Van, Oliveira, Juliana, Vesth, Tammi, Visser, Jaap, Jae-Hyuk Yu, Miaomiao Zhou, Andersen, Mikael, Archer, David, Baker, Scott, Benoit, Isabelle, Brakhage, Axel, Braus, Gerhard, Fischer, Reinhard, Frisvad, Jens, Goldman, Gustavo, Houbraken, Jos, Berl Oakley, Pócsi, István, Scazzocchio, Claudio, Seiboth, Bernhard, VanKuyk, Patricia, Wortman, Jennifer, Dyer, Paul, and Grigoriev, Igor

Abstract

Extended analysis of stress tolerance of the aspergilli. (PDF 706 kb)

Published
2017
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29. Additional file 35: of Comparative genomics reveals high biological diversity and specific adaptations in the industrially and medically important fungal genus Aspergillus

Author

Vries, Ronald De, Riley, Robert, Wiebenga, Ad, Aguilar-Osorio, Guillermo, Sotiris Amillis, Cristiane Uchima, Anderluh, Gregor, Asadollahi, Mojtaba, Askin, Marion, Barry, Kerrie, Battaglia, Evy, Bayram, Özgür, Benocci, Tiziano, Braus-Stromeyer, Susanna, Caldana, Camila, Cánovas, David, Cerqueira, Gustavo, Fusheng Chen, Wanping Chen, Choi, Cindy, Clum, Alicia, Santos, Renato Dos, Damásio, André, Diallinas, George, Emri, Tamás, Fekete, Erzsébet, Flipphi, Michel, Freyberg, Susanne, Gallo, Antonia, Gournas, Christos, Habgood, Rob, Hainaut, Matthieu, Harispe, María, Henrissat, Bernard, Hildén, Kristiina, Hope, Ryan, Hossain, Abeer, Karabika, Eugenia, Karaffa, Levente, Karányi, Zsolt, Kraševec, Nada, Kuo, Alan, Kusch, Harald, LaButti, Kurt, Lagendijk, Ellen, Lapidus, Alla, Levasseur, Anthony, Lindquist, Erika, Lipzen, Anna, Logrieco, Antonio, MacCabe, Andrew, Mäkelä, Miia, Malavazi, Iran, Melin, Petter, Meyer, Vera, Mielnichuk, Natalia, Miskei, Márton, Molnár, Ákos, Mulé, Giuseppina, Chew Ngan, Orejas, Margarita, Orosz, Erzsébet, Ouedraogo, Jean, Overkamp, Karin, Park, Hee-Soo, Perrone, Giancarlo, Francois Piumi, Punt, Peter, Ram, Arthur, Ramón, Ana, Rauscher, Stefan, Record, Eric, Riaño-Pachón, Diego, Robert, Vincent, Röhrig, Julian, Ruller, Roberto, Salamov, Asaf, Nadhira Salih, Samson, Rob, Sándor, Erzsébet, Sanguinetti, Manuel, Schütze, Tabea, Sepčić, Kristina, Shelest, Ekaterina, Sherlock, Gavin, Sophianopoulou, Vicky, Squina, Fabio, Sun, Hui, Susca, Antonia, Todd, Richard, Tsang, Adrian, Unkles, Shiela, Wiele, Nathalie Van De, Rossen-Uffink, Diana Van, Oliveira, Juliana, Vesth, Tammi, Visser, Jaap, Jae-Hyuk Yu, Miaomiao Zhou, Andersen, Mikael, Archer, David, Baker, Scott, Benoit, Isabelle, Brakhage, Axel, Braus, Gerhard, Fischer, Reinhard, Frisvad, Jens, Goldman, Gustavo, Houbraken, Jos, Berl Oakley, Pócsi, István, Scazzocchio, Claudio, Seiboth, Bernhard, VanKuyk, Patricia, Wortman, Jennifer, Dyer, Paul, and Grigoriev, Igor

Abstract

Maximal likelihood phylogeny of MASC1/RID1 orthologs. (PDF 115 kb)

Published
2017
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30. Comparative genomics reveals high biological diversity and specific adaptations in the industrially and medically important fungal genus Aspergillus

Author

de Vries, Ronald P., Riley, Robert, Wiebenga, Ad, Aguilar-Osorio, Guillermo, Amillis, Sotiris, Uchima, Cristiane Akemi, Anderluh, Gregor, Asadollahi, Mojtaba, Askin, Marion, Barry, Kerrie W, Battaglia, Evy, Bayram, Ozgur, Benocci, Tiziano, Braus-Stromeyer, Susanna A., Caldana, Camila, Canovas, David, Cerqueira, Gustavo C., Chen, Fusheng, Chen, Wanping, Choi, Cindy, Clum, Alicia, Correa dos Santos, Renato Augusto, de Lima Damasio, Andre Ricardo, Diallinas, George, Emri, Tamas, Fekete, Erzsebet, Flipphi, Michel, Freyberg, Susanne, Gallo, Antonia, Gournas, Christos, Habgood, Rob, Hainaut, Matthieu, Laura Harispe, Maria, Henrissat, Bernard, Hilden, Kristiina S., Hope, Ryan, Hossain, Abeer, Karabika, Eugenia, Karaffa, Levente, Karanyi, Zsolt, Krasevec, Nada, Kuo, Alan, Kusch, Harald, LaButti, Kurt M, Lagendijk, Ellen L., Lapidus, Alla, Levasseur, Anthony, Lindquist, Erika A, Lipzen, Anna M, Logrieco, Antonio F., Maccabe, Andrew, Makela, Miia R., Malavazi, Iran, Melin, Petter, Meyer, Vera, Mielnichuk, Natalia, Miskei, Marton, Molnar, Akos P., Mule, Giuseppina, Ngan, Chew Yee, Orejas, Margarita, Orosz, Erzsebet, Ouedraogo, Jean Paul, Overkamp, Karin M, Park, Hee-Soo, Perrone, Giancarlo, Piumi, Francois, Punt, Peter J, Ram, Arthur F J, Ramon, Ana, Rauscher, Stefan, Record, Eric, Riano-Pachon, Diego Mauricio, Robert, Vincent, Roehrig, Julian, Ruller, Roberto, Salamov, Asaf, Salih, Nadhira S., Samson, Rob A., Sandor, Erzsebet, Sanguinetti, Manuel, Schutze, Tabea, Sepcic, Kristina, Shelest, Ekaterina, Sherlock, Gavin, Sophianopoulou, Vicky, Squina, FabioM., Sun, Hui, Susca, Antonia, Todd, Richard B, Tsang, Adrian, Unkles, Shiela E., van de Wiele, Nathalie, van Rossen-Uffink, Diana, de Castro Oliveira, Juliana Velasco, Vesth, Tammi C., Visser, Jaap, Yu, Jae-Hyuk, Zhou, Miaomiao, Andersen, Mikael R, Archer, David B., Baker, Scott E, Benoit, Isabelle, Brakhage, Axel A, Braus, Gerhard H., Fischer, Reinhard, Frisvad, Jens C., Goldman, Gustavo H., Houbraken, Jos, Oakley, Berl R, Pocsi, Istvan, Scazzocchio, Claudio, Seiboth, Bernhard, vanKuyk, Patricia A, Wortman, Jennifer Russo, Dyer, Paul S, Grigoriev, Igor V, de Vries, Ronald P., Riley, Robert, Wiebenga, Ad, Aguilar-Osorio, Guillermo, Amillis, Sotiris, Uchima, Cristiane Akemi, Anderluh, Gregor, Asadollahi, Mojtaba, Askin, Marion, Barry, Kerrie W, Battaglia, Evy, Bayram, Ozgur, Benocci, Tiziano, Braus-Stromeyer, Susanna A., Caldana, Camila, Canovas, David, Cerqueira, Gustavo C., Chen, Fusheng, Chen, Wanping, Choi, Cindy, Clum, Alicia, Correa dos Santos, Renato Augusto, de Lima Damasio, Andre Ricardo, Diallinas, George, Emri, Tamas, Fekete, Erzsebet, Flipphi, Michel, Freyberg, Susanne, Gallo, Antonia, Gournas, Christos, Habgood, Rob, Hainaut, Matthieu, Laura Harispe, Maria, Henrissat, Bernard, Hilden, Kristiina S., Hope, Ryan, Hossain, Abeer, Karabika, Eugenia, Karaffa, Levente, Karanyi, Zsolt, Krasevec, Nada, Kuo, Alan, Kusch, Harald, LaButti, Kurt M, Lagendijk, Ellen L., Lapidus, Alla, Levasseur, Anthony, Lindquist, Erika A, Lipzen, Anna M, Logrieco, Antonio F., Maccabe, Andrew, Makela, Miia R., Malavazi, Iran, Melin, Petter, Meyer, Vera, Mielnichuk, Natalia, Miskei, Marton, Molnar, Akos P., Mule, Giuseppina, Ngan, Chew Yee, Orejas, Margarita, Orosz, Erzsebet, Ouedraogo, Jean Paul, Overkamp, Karin M, Park, Hee-Soo, Perrone, Giancarlo, Piumi, Francois, Punt, Peter J, Ram, Arthur F J, Ramon, Ana, Rauscher, Stefan, Record, Eric, Riano-Pachon, Diego Mauricio, Robert, Vincent, Roehrig, Julian, Ruller, Roberto, Salamov, Asaf, Salih, Nadhira S., Samson, Rob A., Sandor, Erzsebet, Sanguinetti, Manuel, Schutze, Tabea, Sepcic, Kristina, Shelest, Ekaterina, Sherlock, Gavin, Sophianopoulou, Vicky, Squina, FabioM., Sun, Hui, Susca, Antonia, Todd, Richard B, Tsang, Adrian, Unkles, Shiela E., van de Wiele, Nathalie, van Rossen-Uffink, Diana, de Castro Oliveira, Juliana Velasco, Vesth, Tammi C., Visser, Jaap, Yu, Jae-Hyuk, Zhou, Miaomiao, Andersen, Mikael R, Archer, David B., Baker, Scott E, Benoit, Isabelle, Brakhage, Axel A, Braus, Gerhard H., Fischer, Reinhard, Frisvad, Jens C., Goldman, Gustavo H., Houbraken, Jos, Oakley, Berl R, Pocsi, Istvan, Scazzocchio, Claudio, Seiboth, Bernhard, vanKuyk, Patricia A, Wortman, Jennifer Russo, Dyer, Paul S, and Grigoriev, Igor V

Published
2017

31. Comparisons of the Arabidopsis thaliana High-affinity Nitrate Transporter Complex AtNRT2.1/AtNAR2.1 and the Aspergillus nidulans AnNRTA: structure function considerations

Author

Kotur, Zorica, Unkles, Shiela E., and Glass, Anthony D. M.

Abstract

The high-affinity nitrate transporter of green plants is composed of two polypeptides, NRT2.1 and NAR2.1, while in fungi it appears that nitrate influx is mediated by NRT2 alone. Another difference between plants and fungi is that the central (cytoplasmic) loop of the 12 membrane spanning regions of NRT is quite large in fungi, consisting of 91 amino acid residues, compared with the relatively short (21 amino acid residues) plant NRT2.1. Here we examine potential amino acid residues involved in the plant NRT2.1:NAR2.1 association by mutation of conserved amino acids in Arabidopsis thaliana AtNRT2.1. Only the replacement of leucine 85 by glutamine disrupted the association between AtNRT2.1 and AtNAR2.1, as examined using the yeast two-hybrid system. Further, to investigate the nitrate-transporting function of AtNRT2.1 in a context free of other members of the NRT2 family, we expressed AtNRT2.1 in Aspergillus nidulans. In the fungal context the plant NRT alone was capable of restoring nitrate transport to a nitrate transport defective mutant, but only when the AtNRT2.1 central loop was replaced by its fungal counterpart.

Published
2016
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38. Comparisons of the Arabidopsis thaliana High-affinity Nitrate Transporter Complex AtNRT2.1/AtNAR2.1 and the Aspergillus nidulans AnNRTA: structure function considerations.

Author

Kotur, Zorica, Unkles, Shiela E., and Glass, Anthony D. M.

Subjects
*ARABIDOPSIS thaliana, *NITRATE transporters, *ASPERGILLUS nidulans, *POLYPEPTIDES, *AMINO acid residues, *GLUTAMINE
Abstract

The high-affinity nitrate transporter of green plants is composed of two polypeptides, NRT2.1 and NAR2.1, while in fungi it appears that nitrate influx is mediated by NRT2 alone. Another difference between plants and fungi is that the central (cytoplasmic) loop of the 12 membrane spanning regions of NRT is quite large in fungi, consisting of 91 amino acid residues, compared with the relatively short (21 amino acid residues) plant NRT2.1. Here we examine potential amino acid residues involved in the plant NRT2.1:NAR2.1 association by mutation of conserved amino acids in Arabidopsis thaliana AtNRT2.1. Only the replacement of leucine 85 by glutamine disrupted the association between AtNRT2.1 and AtNAR2.1, as examined using the yeast two-hybrid system. Further, to investigate the nitrate-transporting function of AtNRT2.1 in a context free of other members of the NRT2 family, we expressed AtNRT2.1 in Aspergillus nidulans. In the fungal context the plant NRT alone was capable of restoring nitrate transport to a nitrate transport defective mutant, but only when the AtNRT2.1 central loop was replaced by its fungal counterpart. [ABSTRACT FROM AUTHOR]

Published
2017
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