1. Evaluating the Recombinant T24H Enzyme-Linked Immunoelectrotransfer Blot Assay for the Diagnosis of Neurocysticercosis in a Panel of Samples from a Large Community-Based Randomized Control Trial in 60 Villages in Burkina Faso
- Author
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Kathleen Breen, Vivian Richter, Sukwan Handali, M. P. Boncoeur-Martel, Rabiou Cissé, Athanase Millogo, Assana Cissé, Zekiba Tarnagda, Sarah Gabriël, Pierre Dorny, Rasmané Ganaba, Andrea Sylvia Winkler, John Noh, Anke Van Hul, Hélène Carabin, Veronique Dermauw, Pierre-Marie Preux, Department of Biomedical Sciences [Antwerp], Institute of Tropical Medicine [Antwerp] (ITM), Department of Biostastistics and Epidemiology [University of Oklahoma Health Sciences Center], University of Oklahoma (OU)-University of Oklahoma Health Sciences Center (OUHSC), Neuroépidémiologie Tropicale (NET), CHU Limoges-Institut d'Epidémiologie Neurologique et de Neurologie Tropicale-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST), Université de Limoges (UNILIM)-Université de Limoges (UNILIM), Centre Hospitalier Universitaire Souro Sanou [Bobo-Dioulasso] (CHUSS), Agence de Formation de Recherche et d'expertise en Santé pour l'Afrique (AFRICSanté), Laboratoire de Biostatistique et d'Informatique Médicale, Université de Limoges (UNILIM), Service de l'Information Médicale et de l'Évaluation [CHU Limoges] (SIME), CHU Limoges, Service de Neuroradiologie interventionnelle [CHU Limoges], Research Group Veterinary Public Health and Zoonoses, Department of Veterinary Public Health and Food Safety, Laboratorium of Chemical Analysis, Universiteit Gent = Ghent University [Belgium] (UGENT), Institute of Tropical Medicine [Antwerp] ( ITM ), University of Oklahoma ( OU ) -University of Oklahoma Health Sciences Center ( OUHSC ), Neuroépidémiologie Tropicale ( NET ), Institut Génomique, Environnement, Immunité, Santé, Thérapeutique ( GEIST ), Université de Limoges ( UNILIM ) -Université de Limoges ( UNILIM ) -CHU Limoges-Institut d'Epidémiologie Neurologique et de Neurologie Tropicale-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Centre Hospitalier Universitaire Souro Sanou [Bobo-Dioulasso] ( CHUSS ), Agence de Formation de Recherche et d'expertise en Santé pour l'Afrique ( AFRICSanté ), Université de Limoges ( UNILIM ), Service de l'Information Médicale et de l'Évaluation [CHU Limoges] ( SIME ), and Ghent University [Belgium] ( UGENT )
- Subjects
Adult ,Male ,Rural Population ,medicine.medical_specialty ,030231 tropical medicine ,Neurocysticercosis ,CYSTICERCOSIS ,Enzyme-Linked Immunosorbent Assay ,Gastroenterology ,ANTIGENS ,Serology ,law.invention ,03 medical and health sciences ,0302 clinical medicine ,Antigen ,Randomized controlled trial ,law ,Seizures ,Virology ,Internal medicine ,Surveys and Questionnaires ,Taenia solium ,parasitic diseases ,medicine ,Medicine and Health Sciences ,Animals ,Humans ,030212 general & internal medicine ,business.industry ,TAENIA-SOLIUM ,Headache ,Cysticercosis ,[ SDV.SPEE ] Life Sciences [q-bio]/Santé publique et épidémiologie ,Articles ,medicine.disease ,3. Good health ,Blot ,medicine.drug_formulation_ingredient ,Infectious Diseases ,AGREEMENT ,Recombinant DNA ,Parasitology ,Female ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie ,business ,Tomography, X-Ray Computed - Abstract
Current guidelines for the diagnosis of neurocysticercosis (NCC) recommend the use of the lentil lectin-bound glycoprotein enzyme-linked immunoelectrotransfer blot assay (LLGP-EITB) as the reference standard for serological testing. In response to the drawbacks involved with the use of the LLGP-EITB, a recombinant T24H antigen (rT24H) EITB assay was developed, with promising results. However, the test has yet to be evaluated among individuals from sub-Saharan Africa (SSA). The aim of the present study was to investigate the performance of the rT24H EITB assay for the detection of NCC cases in a panel of serum samples (N = 366, of which 173 patients presented with epileptic seizures and/or severe chronic headaches, and 193 matched manifestation-free participants) collected as part of a large community-based trial in Burkina Faso. A perfect agreement between the rT24H EITB and the native gp24 (and its homodimer, gp42) LLGP-EITB was found (kappa value of 1.0). Furthermore, among patients with the neurological manifestations of interest who underwent a computed tomography scan, the rT24H EITB and native antigen LLGP-EITB had a comparable ability to correctly identify NCC cases with multiple viable (rT24H: sensitivity: 80.0%), single viable (66.7%), and calcified/degenerating cysts only (25.0%), albeit for multiple viable and calcified cysts, the rT24H estimated sensitivity seemed lower, but more uncertain, than previously reported. The rT24H EITB specificity was high (98.2%) and in line with previous studies. This study confirms the value of the recombinant rT24H EITB as an alternative to the native antigen LLGP-EITB for the diagnosis of NCC in a SSA community setting.
- Published
- 2018