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451 results on '"University of Leon"'

1. Evolution of the clusters of genes for β-lactam antibiotics: a model for evolutive combinatorial assembly of new β-lactams

Author: Paloma Liras; Area of Microbiology, Faculty of Biology, University of Leon, Spain, Antonio Rodríguez-García; Area of Microbiology, Faculty of Biology, University of Leon, Spain, Juan F. Martín; Area of Microbiology, Faculty of Biology, University of Leon, Spain, Paloma Liras; Area of Microbiology, Faculty of Biology, University of Leon, Spain, Antonio Rodríguez-García; Area of Microbiology, Faculty of Biology, University of Leon, Spain, and Juan F. Martín; Area of Microbiology, Faculty of Biology, University of Leon, Spain
Abstract: β-Lactam antibiotics are produced by prokaryotic and eukaryotic organisms. The genes for β-lactam biosynthesis are organized in clusters but the location of the different genes is not identical. Biosynthesis genes are clustered with genes for resistance (bla, pbp) and for the efflux of the antibiotic (cmcT) in prokaryotes. Comparison of proteins reveals much larger differences for primary metabolism enzymes than for β-lactam biosynthesis enzymes in producing organisms. This suggests a horizontal transfer of the β-lactam antibiotic biosynthesis genes.
Published: 2010

2. Gene clusters for β-lactam antibiotics and control of their expression: why have clusters evolved, and from where did they originate?

Author: Paloma Liras; Biotechnological Institute, Scientific Park of Leon, Spain, and Microbiology Area, Faculty of Biological and Environmental Sciences, University of Leon, Spain, Juan F. Martín; Biotechnological Institute, Scientific Park of Leon, Spain, and Microbiology Area, Faculty of Biological and Environmental Sciences, University of Leon, Spain, Paloma Liras; Biotechnological Institute, Scientific Park of Leon, Spain, and Microbiology Area, Faculty of Biological and Environmental Sciences, University of Leon, Spain, and Juan F. Martín; Biotechnological Institute, Scientific Park of Leon, Spain, and Microbiology Area, Faculty of Biological and Environmental Sciences, University of Leon, Spain
Abstract: While β-lactam compounds were discovered in filamentous fungi, actinomycetes and gram-negative bacteria are also known to produce different types of β-lactams. All β-lactam compounds contain a four-membered β-lactam ring. The structure of their second ring allows these compounds to be classified into penicillins, cephalosporins, clavams, carbapenens or monobactams. Most β-lactams inhibits bacterial cell wall biosynthesis but others behave as β-lactamase inhibitors (e.g., clavulanic acid) and even as antifungal agents (e.g., some clavams). Due to the nature of the second ring in β-lactam molecules, the precursors and biosynthetic pathways of clavams, carbapenems and monobactams differ from those of penicillins and cephalosporins. These last two groups, including cephamycins and cephabacins, are formed from three precursor amino acids that are linked into the α-aminoadipyl-L-cysteinyl-D-valine tripeptide. The first two steps of their biosynthetic pathways are common. The intermediates of these pathways, the characteristics of the enzymes involved, the lack of introns in the genes and bioinformatic analysis suggest that all of them should have evolved from an ancestral gene cluster of bacterial origin, which was surely transferred horizontally in the soil from producer to non-producer microorganisms. The receptor strains acquired fragments of the original bacterial cluster and occasionally inserted new genes into the clusters, which once modified, acquired new functions and gave rise to the final compounds that we know. When the order of genes in the Streptomyces genome is analyzed, the antibiotic gene clusters are highlighted as gene islands in the genome. Nonetheless, the assemblage of the ancestral β-lactam gene cluster remains a matter of speculation. [Int Microbiol 2006; 9(1):9-19]
Published: 2010

3. Effects of the intense summer desiccation and the autumn filling on the water chemistry in some Mediterranean ponds

Author: Margarita Fernandez-Alaez; University of Leon, Camino Fernandez-Alaez, Margarita Fernandez-Alaez; University of Leon, and Camino Fernandez-Alaez
Abstract: The objective of this research was to compare the effects of evaporation and total desiccation, as well as the influence of the rate of relling, on the concentrations of major ions and nutrients (nitrogen and phosphorus) in Mediterranean ponds. Water level uctuations in these ecosystems can have a decisive role in community structuring and functioning and may affect their conservation values. The results obtained enable an assessment of the consequences of water quality changes resulting from the increased evaporation, drastic fluctuations in water levels and higher incidence of droughts in summer. Desiccation was observed to cause an increase in the bicarbonate and chloride concentrations, whilst sulphate content was frequently found to have decreased. With respect to nutrients, a drastic reduction in water volume increased orthophosphate levels, with a consequent drop in the N-nitrate: SRP ratio. Pond refilling following total desiccation resulted in higher total anion content, especially among the waters with higher mineral content, the extent of which was related to the rate of refilling. When refill followed desiccation, sulphate concentrations increased the most, whilst alkalinity decreased in most ponds. An increase in chloride concentration was only observed when refilling occurred slowly. Orthophosphate and nitrate concentrations increased at the start of the refilling period, although the most pronounced increase was in nitrate which, together with potassium, was probably washed into the ponds through soil leaching following autumn rainfall.
Published: 2010

4. Re-branding Colombia through Urban Transformation and Rural Regional Marketing

Author: Norberto Muñiz Martínez and University of Leon, Faculty of Economics and Business
Subjects: Medellín, 05 social sciences, 0211 other engineering and technologies, urban transformation, 021107 urban & regional planning, 02 engineering and technology, Transformation (music), medellín, Political science (General), Political science, Rebranding, 0502 economics and business, cultural service ecosystem, 050211 marketing, Economic geography, rebranding, JA1-92
Abstract: Colombia is now projecting a new, positive image to the world after overcoming a past characterised by politically inspired guerrilla warfare and violent conflict with narco-trafficking cartels which had ravaged the country for decades. Even before the country’s transformation, other intermediate place institutions – cities and regions – had already taken significant steps towards territorial change and marketing. This paper outlines the processes involved in urban and social transformation in the city of Medellín and in the marketing of the coffee region, as illustrative cases of city re-branding and regional branding, respectively.
Published: 2019

5. Thermogravimetric analysis of biowastes during combustion

Author: Moran, A [Department of Chemical Engineering, Institute of Natural Resources, University of Leon, Avenida de Portugal, 41, 24071 Leon (Spain)]
Published: 2010
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6. The role of TNFR2 and DR3 in the in vivo expansion of tregs in T Cell depleting transplantation regimens

Author: Pascal Schneider, Leo Buhler, Luis Graca, Jose-Ignacio Rodriguez-Barbosa, José A. Pérez-Simón, Maria-Luisa del Rio, Repositório da Universidade de Lisboa, European Commission, Instituto de Salud Carlos III, Ministerio de Sanidad, Servicios Sociales e Igualdad (España), Junta de Castilla y León, Swiss National Science Foundation, Centro de Investigación Biomédica en Red Cáncer (España), Universidad de Sevilla. Departamento de Medicina, European Union ERDF/ESF, Fondo de Investigaciones Sanitarias, Ministry of Health, Spanish Government, Gerencia Regional de Salud, Spanish National Network CIBER-ONC (oncology research), Unit of Excellence Research UIC (Department of Education of the Regional Government, Junta de Castilla y Leon) 012, [Rodriguez-Barbosa,JI, del Rio,ML] Transplantation Immunobiology, School of Biology and Biotechnology, Institute of Molecular Biology, Genomics and Proteomics, University of Leon, Leon, Spain. [Schneider,P] Department of Biochemistry, University of Lausanne, Epalinges, Switzerland. [Graca,L] School of Medicine, Institute of Molecular Medicine, University of Lisbon, Lisbon, Portugal. [Bühler L] Faculty of Science and Medicine, Section of Medicine, University of Fribourg, Fribourg, Switzerland. [Perez-Simon,JA] Department of Hematology, Institute of Biomedicine (IBIS/CSIC), University Hospital Virgen del Rocio, Sevilla, Spain, and This work has been supported by grant FIS PI13/00029 (Fondo de Investigaciones Sanitarias, Ministry of Health, Spanish Government and co-funded by European Union ERDF/ESF, 'Investing in your future'), LE093U13 and Unit of Excellence Research UIC #012 (Department of Education of the Regional Government, Junta de Castilla y Leon), and Gerencia Regional de Salud (BIO/01/15) to JIRB. It was also funded by FIS PI16/00002 (Instituto de Salud Carlos III and co-funded by European Union ERDF/ESF, 'Investing in your future') and Gerencia Regional de Salud GRS963/A/2014, GRS1142/A/2015 and GRS 1505/A/2017 to M.L.R.G. This work has been supported with funding from the Spanish National Network CIBER-ONC (oncology research) CB16/12/00480 to JAPS. PS is supported by the Swiss National Science Foundation (grant 31003A-176526).
Subjects: 0301 basic medicine, Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Differentiation [Medical Subject Headings], Transplantation Conditioning, Anatomy::Cells::Blood Cells::Leukocytes::Leukocytes, Mononuclear::Lymphocytes::T-Lymphocytes::T-Lymphocyte Subsets::T-Lymphocytes, Regulatory [Medical Subject Headings], medicine.medical_treatment, Phenomena and Processes::Physiological Phenomena::Physiological Processes::Growth and Development::Growth::Cell Growth Processes::Cell Proliferation::Cell Division [Medical Subject Headings], Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Membrane Proteins::Receptors, Cell Surface::Receptors, Immunologic::Receptors, Cytokine::Receptors, Tumor Necrosis Factor::Receptors, Tumor Necrosis Factor, Member 25 [Medical Subject Headings], Review, Anatomy::Body Regions::Transplants::Allografts [Medical Subject Headings], T-Lymphocytes, Regulatory, Graft-versus-host disease, Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings], Chemicals and Drugs::Biological Factors::Intercellular Signaling Peptides and Proteins::Tumor Necrosis Factors::Tumor Necrosis Factor-alpha [Medical Subject Headings], lcsh:Chemistry, Mice, 0302 clinical medicine, Organisms::Eukaryota::Animals [Medical Subject Headings], Homeostasis, lcsh:QH301-705.5, Spectroscopy, Bone Marrow Transplantation, Phenomena and Processes::Immune System Phenomena::Immune System Processes::Transplantation Immunology [Medical Subject Headings], Analytical, Diagnostic and Therapeutic Techniques and Equipment::Surgical Procedures, Operative::Transplantation::Organ Transplantation::Heart Transplantation [Medical Subject Headings], Phenomena and Processes::Immune System Phenomena::Immune System Processes::Immunomodulation::Immune Tolerance [Medical Subject Headings], Diseases::Hemic and Lymphatic Diseases::Hematologic Diseases::Leukocyte Disorders::Leukopenia::Lymphopenia [Medical Subject Headings], Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Models, Theoretical::Models, Biological::Models, Immunological [Medical Subject Headings], CD28, Immunosuppression, hemic and immune systems, Cell Differentiation, General Medicine, Regulatory T cells, Allografts, Analytical, Diagnostic and Therapeutic Techniques and Equipment::Therapeutics::Biological Therapy::Immunomodulation::Immunotherapy::Immunosuppression::Transplantation Conditioning [Medical Subject Headings], Adoptive Transfer, Computer Science Applications, Homeostatic proliferation, Tolerance induction, medicine.anatomical_structure, Analytical, Diagnostic and Therapeutic Techniques and Equipment::Surgical Procedures, Operative::Transplantation::Tissue Transplantation::Bone Marrow Transplantation [Medical Subject Headings], surgical procedures, operative, Lymphocyte Transfusion, Graft rejection, Cell Division, Linfocitos T reguladores, Proliferación celular, T cell, TNF/TNF receptors, Enfermedad injerto contra huésped, Catalysis, Lymphocyte Depletion, Inorganic Chemistry, Abatacept, 03 medical and health sciences, Analytical, Diagnostic and Therapeutic Techniques and Equipment::Therapeutics::Biological Therapy::Blood Transfusion::Blood Component Transfusion::Leukocyte Transfusion::Lymphocyte Transfusion [Medical Subject Headings], Immune system, Transplantation Immunology, Lymphopenia, medicine, Immune Tolerance, Animals, Humans, Receptors, Tumor Necrosis Factor, Type II, Physical and Theoretical Chemistry, Linfopenia, Molecular Biology, Receptors, Tumor Necrosis Factor, Member 25, Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Mice [Medical Subject Headings], Transplantation, Rechazo de injerto, business.industry, Tumor Necrosis Factor-alpha, Organic Chemistry, Models, Immunological, Receptores del factor de necrosis tumoral, medicine.disease, Chemicals and Drugs::Biological Factors::Antigens::Antigens, Surface::Antigens, Differentiation::Antigens, CD::Receptors, Tumor Necrosis Factor, Type II [Medical Subject Headings], Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Immunologic Techniques::Immunization::Immunization, Passive::Adoptive Transfer [Medical Subject Headings], 030104 developmental biology, lcsh:Biology (General), lcsh:QD1-999, Cancer research, Trasplante, Heart Transplantation, Phenomena and Processes::Immune System Phenomena::Immune System Processes::Transplantation Immunology::Host vs Graft Reaction::Graft Rejection [Medical Subject Headings], Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Immunologic Techniques::Immunosuppression::Lymphocyte Depletion [Medical Subject Headings], business, Phenomena and Processes::Physiological Phenomena::Physiological Processes::Homeostasis [Medical Subject Headings], 030215 immunology
Abstract: This article belongs to the Special Issue Trends in Molecular Research for Transplantation Immunology., Regulatory T cells (Tregs) are essential for the maintenance of tolerance to self and non-self through cell-intrinsic and cell-extrinsic mechanisms. Peripheral Tregs survival and clonal expansion largely depend on IL-2 and access to co-stimulatory signals such as CD28. Engagement of tumor necrosis factor receptor (TNFR) superfamily members, in particular TNFR2 and DR3, contribute to promote peripheral Tregs expansion and sustain their survival. This property can be leveraged to enhance tolerance to allogeneic transplants by tipping the balance of Tregs over conventional T cells during the course of immune reconstitution. This is of particular interest in peri-transplant tolerance induction protocols in which T cell depletion is applied to reduce the frequency of alloreactive T cells or in conditioning regimens that allow allogeneic bone marrow transplantation. These conditioning regimens are being implemented to limit long-term side effects of continuous immunosuppression and facilitate the establishment of a state of donor-specific tolerance. Lymphopenia-induced homeostatic proliferation in response to cytoreductive conditioning is a window of opportunity to enhance preferential expansion of Tregs during homeostatic proliferation that can be potentiated by agonist stimulation of TNFR., This work has been supported by grant FIS PI13/00029 (Fondo de Investigaciones Sanitarias, Ministry of Health, Spanish Government and co-funded by European Union ERDF/ESF, “Investing in your future”), LE093U13 and Unit of Excellence Research UIC #012 (Department of Education of the Regional Government, Junta de Castilla y Leon), and Gerencia Regional de Salud (BIO/01/15) to JIRB. It was also funded by FIS PI16/00002 (Instituto de Salud Carlos III and co-funded by European Union ERDF/ESF, “Investing in your future”) and Gerencia Regional de Salud GRS963/A/2014, GRS1142/A/2015 and GRS 1505/A/2017 to M.L.R.G. This work has been supported with funding from the Spanish National Network CIBER-ONC (oncology research) CB16/12/00480 to JAPS. PS is supported by the Swiss National Science Foundation (grant 31003A-176526).
Published: 2020

7. Association between Polyphenol Intake and Gastric Cancer Risk by Anatomic and Histologic Subtypes: MCC-Spain

Author: Nuria Aragonés, Facundo Vitelli-Storelli, Inés Gómez-Acebo, Gemma Castaño-Vinyals, Eva Adarnaz, Marina Pollán, Raul Zamora-Ros, Juan Alguacil, Estefanía Toledo, Manolis Kogevinas, Antonio J. Molina, José Juan Jiménez-Moleón, Vicente Martín, Ana Molina-Barceló, María Rubín-García, Guillermo Fernández-Tardón, María Dolores Chirlaque, Beatriz Pérez-Gómez, Mireia Obón-Santacana, Instituto de Salud Carlos III, Fundación Marqués de Valdecilla, Ministerio de Economía y Competitividad (España), Red Temática de Investigación Cooperativa en Cáncer (España), Junta de Castilla y León (España), Regional Government of Andalusia (España), Generalitat Valenciana (España), Fundación La Caixa, Basque Government (España), Gobierno de la Región de Murcia (España), Unión Europea. Comisión Europea, Asociación Española Contra el Cáncer, Government of Catalonia (España), Fundación Caja de Ahorros de Asturias, University of Oviedo (España), Ministerio de Educación (España), University of Leon (España), Ministerio de Ciencia e Innovación (España), Junta de Castilla y León, Gobierno de Andalucía, Generalitat Valenciana, Recercaixa, Gobierno Vasco, Gobierno de Murcia, European Commission, Fundación Científica AECC, Generalitat de Catalunya, Universidad de Oviedo, Universidad de León, Universidad de Cantabria, [Rubín-García,M, Vitelli-Storelli,F, Molina,AJ, Martín,V] Group of Investigation in Interactions Gene-Environment and Health (GIIGAS), Institute of Biomedicine (IBIOMED), University of León, León, Spain. [Zamora-Ros,R] Unit of Nutrition and Cancer, Cancer Epidemiology Research Programme, Catalan Institute of Oncology (ICO), Bellvitge Biomedical Research Institute (IDIBELL), L’Hospitalet del Llobregat, Barcelona, Spain. [Aragonés,N] Department of Health of Madrid, Epidemiology Section, Public Health Division, Madrid, Spain. [Aragonés,N, Adarnaz,E, Castaño-Vinyals,G, Gómez-Acebo,I, Fernández-Tardón,G, Jiménez-Moleón,JJ, Alguacil,J, Dolores Chirlaque,MD, Pérez-Gómez,B, Pollán,M, Kogevinas,M, Martín,V] Consortium for Biomedical Research in Epidemiology & Public Health (CIBER en Epidemiología y Salud Pública-CIBERESP), Madrid, Spain. [Adarnaz,E, Toledo,E] Institute for Health Research (IdiSNA), Pamplona, Spain. [Castaño-Vinyals,G, Kogevinas,M] Barcelona Institute for Global Health (ISGlobal), Barcelona, Spain. [Castaño-Vinyals,G, Kogevinas,M] Hospital del Mar Medical Research Institute (IMIM), Barcelona, Spain. [Castaño-Vinyals,G, Kogevinas,M] Department of Public Health, Universitat Pompeu Fabra (UPF), Campus del Mar, Barcelona, Spain. [Obón-Santacana,M] ONCOBELL Program, Bellvitge Biomedical Research Institute (IDIBELL), L’Hospitalet De Llobregat, Barcelona, Spain. [Obón-Santacana,M] Oncology Data Analytics Program (ODAP), Catalan Institute of Oncology (ICO), L’Hospitalet Del Llobregat, Barcelona, Spain. [Gómez-Acebo,I] Facultad de Medicina, Universidad de Cantabria, IDIVAL, Santander, Spain. [Molina-Barceló,A] Cancer and Public Health Area, FISABIO-Public Health, Valencia, Spain. [Fernández-Tardón,G] Health Research Institute of the Principality of Asturias (ISPA), Oncology Institute, University of Oviedo, Oviedo, Asturias. [Jiménez-Moleón,JJ] Instituto de Investigación Biosanitaria de Granada (ibs.GRANADA), Hospitales Universitarios de Granada, Universidad de Granada, Granada, Spain. [Alguacil,J] Centro de Investigación en Recursos Naturales, Salud y Medio Ambiente (RENSMA), Universidad de Huelva, Campus Universitario de El Carmen, Huelva, Spain. [Chirlaque,MD] Department of Epidemiology, Regional Health Council, IMIB-Arrixaca, Murcia University, Campus de Ciencias de la Salud, El Palmar, Murcia, Spain. [Toledo,E] Centro de Investigación Biomédica en Red Fisiopatología de la Obesidad y la Nutrición (CIBEROBN), Institute of Health Carlos III, Madrid, Spain. [Toledo,E] Department of Preventive Medicine and Public Health, University of Navarra, Pamplona, Spain. [Pérez-Gómez,B, Pollán,M] Cancer and Environmental Epidemiology Unit, Department of Epidemiology and Chronic Diseases, National Center for Epidemiology, Carlos III Institute of Health, Madrid, Spain. [Pérez-Gómez,B] Cancer Epidemiology Research Group, Oncology and Hematology Area, IIS Puerta de Hierro, IDIPHIM, Madrid, Spain., and The study was partially funded by the 'Accion Transversal del Cancer', approved by the Spanish Ministry Council on the 11th October 2007, by the Instituto de Salud Carlos III-FEDER (PI08/1770, PI08/0533, PI08/1359, PS09/00773-Cantabria, PS09/01286-León, PS09/01903-Valencia, PS09/02078-Huelva, PS09/01662-Granada, PI11/01403, PI11/01889-FEDER, PI11/00226, PI11/01810, PI11/02213, PI12/00488, PI12/00265, PI12/01270, PI12/00715, PI12/00150, PI14/01219, PI14/0613, PI15/00069, PI15/00914, PI15/01032, PI17CIII/00034), by the Fundación Marqués de Valdecilla (API 10/09), by the ICGC International Cancer Genome Consortium CLL (The ICGC CLL-Genome Project is funded by Spanish Ministerio de Economía y Competitividad (MINECO) through the Instituto de Salud Carlos III (ISCIII) and Red Temática de Investigación del Cáncer (RTICC) del ISCIII (RD12/0036/0036)), by the Junta de Castilla y León (LE22A10-2), by the Consejería de Salud of the Junta de Andalucía (PI-0571-2009, PI-0306-2011, salud201200057018tra), by the Conselleria de Sanitat of the Generalitat Valenciana (AP_061/10), by the Recercaixa (2010ACUP 00310), by the Regional Government of the Basque Country, by the Consejería de Sanidad de la Región de Murcia, by the European Commission grants FOOD-CT-2006-036224-HIWATE, by the Spanish Association Against Cancer (AECC) Scientific Foundation, by the Catalan Government Agency for Management of University and Research Grants (AGAUR) grants 2017SGR723 and 2014SGR850, by the Fundación Caja de Ahorros de Asturias and by the University of Oviedo. IDIBELL is a member of the CERCA Programme, Generalitat de Catalunya. R.Z.-R. was supported by the 'Miguel Servet' program (CP15/00100) from the Institute of Health Carlos III (Co-funded by the European Social Fund (ESF)-ESF investing in your future). M.R.-G., is supported by the Ministry of Education of Spain (FPU17/06488) and by University of León. ISGlobal acknowledges support from the Spanish Ministry of Science and Innovation through the 'Centro de Excelencia Severo Ochoa 2019–2023' Program (CEX2018-000806-S) and support from the Generalitat de Catalunya through the CERCA Program.
Subjects: 0301 basic medicine, Male, stilbenes, Logistic regression, Gastroenterology, Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Statistics as Topic::Probability::Risk::Risk Factors [Medical Subject Headings], Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings], chemistry.chemical_compound, Eating, 0302 clinical medicine, Estilbenos, Risk Factors, Epidemiology, histologic, Odds Ratio, Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Statistics as Topic::Probability::Odds Ratio [Medical Subject Headings], Prospective cohort study, Persons::Persons::Age Groups::Adult::Aged [Medical Subject Headings], Nutrition and Dietetics, Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Epidemiologic Study Characteristics as Topic::Epidemiologic Studies::Case-Control Studies [Medical Subject Headings], Stomach, lignans, MCC-Spain, Middle Aged, anatomic, Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Statistics as Topic::Models, Statistical::Logistic Models [Medical Subject Headings], Quartile, 030220 oncology & carcinogenesis, Dieta, Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Data Collection::Health Surveys::Nutrition Surveys::Diet Surveys [Medical Subject Headings], Female, epidemiology, Chemicals and Drugs::Organic Chemicals::Hydrocarbons::Hydrocarbons, Cyclic::Hydrocarbons, Aromatic::Benzene Derivatives::Benzyl Compounds::Lignans [Medical Subject Headings], lcsh:Nutrition. Foods and food supply, medicine.medical_specialty, Neoplasias gástricas, Polifenoles, Stomach cancer, Chemicals and Drugs::Organic Chemicals::Hydrocarbons::Hydrocarbons, Cyclic::Hydrocarbons, Aromatic::Benzene Derivatives::Phenols::Polyphenols [Medical Subject Headings], Check Tags::Male [Medical Subject Headings], Anatomy::Digestive System::Gastrointestinal Tract::Upper Gastrointestinal Tract::Stomach [Medical Subject Headings], lcsh:TX341-641, Phenomena and Processes::Physiological Phenomena::Nutritional Physiological Phenomena::Diet [Medical Subject Headings], Diet Surveys, Article, 03 medical and health sciences, Stomach Neoplasms, Internal medicine, medicine, Epidemiología, Humans, Compuestos fenólicos, Epidemiologia, Nutrició, Phenomena and Processes::Physiological Phenomena::Nutritional Physiological Phenomena::Nutrition Processes::Eating [Medical Subject Headings], polyphenols, Nutrition, Aged, Geographical Locations::Geographic Locations::Europe::Spain [Medical Subject Headings], business.industry, Diseases::Neoplasms::Neoplasms by Site::Digestive System Neoplasms::Gastrointestinal Neoplasms::Stomach Neoplasms [Medical Subject Headings], Càncer d'estómac, gastric cancer, Cancer, Lignanos, Persons::Persons::Age Groups::Adult::Middle Aged [Medical Subject Headings], Odds ratio, Phenolic acid, medicine.disease, Diet, 030104 developmental biology, Logistic Models, Check Tags::Female [Medical Subject Headings], chemistry, Polyphenol, Spain, Case-Control Studies, business, diet, phenolic acids, Food Science
Abstract: Several anticancer properties have been largely attributed to phenolics in in vivo and in vitro studies, but epidemiologic evidence is still scarce. Furthermore, some classes have not been studied in relation to gastric cancer (GC). The aim of this study was to assess the relationship between the intake of phenolic acids, stilbenes, and other phenolics and the risk of developing GC and its anatomical and histological subtypes. We used data from a multi-case-control study (MCC-Spain) obtained from di erent regions of Spain. We included 2700 controls and 329 GC cases. Odds ratios (ORs) were calculated using mixed e ects logistic regression considering quartiles of phenolic intake. Our results showed an inverse association between stilbene and lignan intake and GC risk (ORQ4 vs. Q1 = 0.47; 95% CI: 0.32–0.69 and ORQ4 vs. Q1 = 0.53; 95% CI: 0.36–0.77, respectively). We found no overall association between total phenolic acid and other polyphenol class intake and GC risk. However, hydroxybenzaldehydes (ORQ4 vs. Q1 = 0.41; 95% CI: 0.28–0.61), hydroxycoumarins (ORQ4 vs. Q1 = 0.49; 95% CI: 0.34–0.71), and tyrosols (ORQ4 vs. Q1 = 0.56; 95% CI: 0.39–0.80) were inversely associated with GC risk. No di erences were found in the analysis by anatomical or histological subtypes. In conclusion, a diet high in stilbenes, lignans, hydroxybenzaldehydes, hydroxycoumarins, and tyrosols was associated with a lower GC risk. Further prospective studies are needed to confirm our results., Instituto de Salud Carlos III, Instituto de Salud Carlos III European Union (EU) PI08/1770 PI08/0533 PI08/1359 PS09/00773-Cantabria PS09/01286-Leon PS09/01903-Valencia PS09/02078-Huelva PS09/01662-Granada PI11/01403 PI11/01889-FEDER PI11/00226 PI11/01810 PI11/02213 PI12/00488, Instituto de Salud Carlos III API 10/09, ICGC International Cancer Genome Consortium CLL (Spanish Ministerio de Economia y Competitividad (MINECO) through the Instituto de Salud Carlos III (ISCIII)), ICGC International Cancer Genome Consortium CLL (Spanish Ministerio de Economia y Competitividad (MINECO) through Red Tematica de Investigacion del Cancer (RTICC) del ISCIII) RD12/0036/0036, Junta de Castilla y León LE22A10-2, Junta de Andalucía PI-0571-200 PI-0306-2011 salud201200057018tra, Conselleria de Sanitat of the Generalitat Valenciana AP_061/10, La Caixa Foundation 2010ACUP 00310, Regional Government of the Basque Country, Consejería de Sanidad de la Región de Murcia, European Commission European Commission Joint Research Centre FOOD-CT-2006-036224-HIWATE, Spanish Association Against Cancer (AECC) Scientific Foundation, Catalan Government Agency for Management of University and Research Grants (AGAUR) grants 2017SGR723 2014SGR850, Fundación Caja de Ahorros de Asturias, University of Oviedo, "Miguel Servet" program from the Institute of Health Carlos III (European Social Fund (ESF)-ESF investing in your future) CP15/00100, Ministry of Education of Spain FPU17/06488, University of Leon, Spanish Ministry of Science and Innovation through the "Centro de Excelencia Severo Ochoa 2019-2023" Program CEX2018-000806-S, Generalitat de Catalunya through the CERCA Program, The Instituto de Salud Carlos III-FEDER PI12/00265 PI12/01270 PI12/00715 PI12/00150 PI14/01219 PI14/0613 PI15/00069 PI15/00914 PI15/01032 PI17CIII/00034
Published: 2020

8. Treatment Patterns and Use of Resources in Patients With Tuberous Sclerosis Complex: Insights From the TOSCA Registry

Author: Marques, Ruben, Belousoye, Elena, Benedik, Mirjana P., Carter, Tom, Cottin, Vincent, Curatolo, Paolo, Dahlin, Maria, D'Amato, Lisa, d'Augeres, Guillaume Beaure, de Vries, Petrus J., Ferreira, Jose C., Feucht, Martha, Fladrowski, Carla, Hertzberg, Christoph, Jozwiak, Sergiusz, Lawson, John A., Macaya, Alfons, Nabbout, Rima, O'Callaghan, Finbar, Qin, Jiong, Sander, Valentin, Sauter, Matthias, Shah, Seema, Takahashi, Yukitoshi, Touraine, Renaud, Youroukos, Sotiris, Zonnenberg, Bernard, Kingswood, John C., Jansen, Anna C., Shinohara, Nobuo, LIorie, Shigeo, Kubota, Masaya, Tohyama, Jun, Imai, Katsumi, Kaneda, Mari, Kaneko, Hideo, Uchida, Yasushi, Kirino, Tomoko, Endo, Shoichi, Inoue, Yoshikazu, Uruno, Katsuhisa, Serdaroglu, Ayse, Yapici, Zuhal, Anlar, Banu, Altunbasak, Sakir, Lvova, Olga, Belyaev, Oleg Valeryevich, Agranovich, Oleg, Levitina, Elena Vladislavovna, Maksimova, Yulia Vladimirovna, Karas, Antonina, Jiang, Yuwu, Zou, Liping, Xu, Kaifeng, Zhang, Yushi, Luan, Guoming, Zhang, Yuqin, Wang, Yi, Jin, Meiling, Ye, Dingwei, Liao, Weiping, Zhou, Liemin, Liu, Jie, Liao, Jianxiang, Yan, Bo, Deng, Yanchun, Jiang, Li, Liu, Zhisheng, Huang, Shaoping, Li, Hua, Kim, Kijoong, Chen, Pei-Lung, Lee, Hsiu-Fen, Tsai, Jeng-Dau, Chi, Ching-Shiang, Huang, Chao-Ching, Riney, Australia Kate, Yates, Deborah, Kwan, Patrick, Likasitwattanakul, Surachai, Nabangchang, Charcrin, Chomtho, Lunliya Thampratankul Krisnachai, Katanyuwong, Kamornwan, Sriudomkajorn, Somjit, Wilmshurst, Jo, Segel, Reeval, Gilboa, Tal, Tzadok, Michal, Fattal-Valevski, Aviva, Papathanasopoulos, Panagiotis, Papavasiliou, Antigone Syrigou, Giannakodimos, Stylianos, Gatzonis, Stylianos, Pavlou, Evangelos, Tzoufi, Meropi, Vergeer, A. M. M., Dhooghe, Marc, Verhelst, Helene, Roelens, Filip, Nassogne, Marie Cecile, Defresne, Pierre, De Waele, Liesbeth, Leroy, Patricia, Demonceau, Nathalie, Legros, Benjamin, Van Bogaert, Patrick, Ceulemans, Berten, Dom, Lina, Castelnau, Pierre, Martin, Anne De Saint, Riquet, Audrey, Milh, Mathieu, Cances, Claude, Pedespan, Jean-Michel, Ville, Dorothee, Roubertie, Agathe, Auvin, Stephane, Berquin, Patrick, Richelme, Christian, Allaire, Catherine, Gueden, Sophie, Tich, Sylvie Nguyen The, Godet, Bertrand, Falco Rojas, Maria Luz Ruiz, Campistol Planas, Jaume, Martinez Bermejo, Antonio, Smeyers Dura, Patricia, Roldan Aparicio, Susana, Martinez Gonzalez, Maria Jesus, Lopez Pison, Javier, Blanco Barca, Manuel Oscar, Lopez Laso, Eduardo, Alonso Luengo, Olga, Aguirre Rodriguez, Francisco Javier, Malaga Dieguez, Ignacio, Camacho Salas, Ana, Marti Carrera, Itxaso, Martinez Salcedo, Eduardo, Yoldi Petri, Maria Eugenia, Cancho Candela, Ramon, Carrilho, Ines da Conceicao, Vieira, Jose Pedro, da Silva Oliveira Monteiro, Jose Paulo, de Oliveira Ferreira Leao, Miguel Jorge Santos, Marceano Ribeiro Luis, Catarina Sofia, Mendonca, Carla Pires, Endziniene, Milda, Strautmanis, Jurgis, Talvik, Inga, Canevini, Maria Paola, Gambardella, Antonio, Pruna, Dario, Buono, Salvatore, Fontana, Elena, Dalla Bernardina, Bernardo, Burloiu, Carmen, Cosma, Iuliu Stefan Bacos, Vintan, Mihaela Adela, Popescu, Laura, Zitterbart, Karel, Payerova, Jaroslava, Bratsky, Ladislav, Zilinska, Zuzana, Gruber-Sedlmayr, Ursula, Baumann, Matthias, Laberlandt, Edda, Rostasy, Kevin, Pataraia, Ekaterina, Elmslie, Frances, Johnston, Clare Ann, Crawford, Pamela, Uldall, Peter, Uvebrant, Paul, Rask, Olof, Bjoernvold, Marit, Brodtkorb, Eylert, Sloerdahi, Andreas, Solhoff, Ragnar, Jaatun, Martine Sofie Gilje, Mandera, Marek, Radzikowska, Elzbieta Janina, Wysocki, Mariusz, Fischereder, Michael, Kurlemann, Gerhard, Wilken, Bernd, Wiemer-Kruel, Adelheid, Budde, Klemens, Marquard, Klaus, Knuf, Markus, Hahn, Andreas, Hartmann, Hans, Merkenschlager, Andreas, Trollmann, Regina, Consortium, TOSCA, Investigators, TOSCA, Physiotherapy, Human Physiology and Anatomy, Pediatrics, Public Health Sciences, Mental Health and Wellbeing research group, Neurogenetics, De Waele, L, [Marques R] Novartis Farma SpA, Origgio, Italy. Institute of Biomedicine (IBIOMED), University of Leon, León, Spain. [Belousova E] Research and Clinical Institute of Pediatrics, Pirogov Russian National Research Medical University, Moscow, Russia. [Benedik MP] SPS Pediatrična Klinika, Ljubljana, Slovenia. [Carter T] TSA Tuberous Sclerosis Association, Nottingham, United Kingdom. [Cottin V] Hôpital Louis Pradel, Claude Bernard University Lyon 1, Lyon, France. [Curatolo P] Tor Vergata University Hospital, Rome, Italy. [Macaya A] Servei de Pediatria General i Especialitats, Vall d'Hebron Hospital Universitari, Barcelona, Spain, Vall d'Hebron Barcelona Hospital Campus, Novartis Pharma AG, Universidad de León [León], Pirogov Russian National Reasearch Medical University Moscow, University Medical Centre Ljubljana [Ljubljana, Slovenia] (UMCL), TSA Tuberous Sclerosis Association, Nottingham, United Kingdom, Hôpital Louis Pradel [CHU - HCL], Hospices Civils de Lyon (HCL), Department of Public Health and Cell Biology, University of Rome Tor Vergata, Via Montpellier 1, 00133 Rome, Italy, Karolinska Institutet [Stockholm], Association Sclérose Tubéreuse de Bourneville (Gradignan), University of Cape Town, Centro Hospitalar de Lisboa Central E.P.E, Medizinische Universität Wien = Medical University of Vienna, Universitätsklinik für Kinder-und Jugendheilkunde, Vivantes Klinikum Neukölln [Berlin, Germany] (VKN), Medical University of Warsaw - Poland, Sydney Children's hospital, Fundació Institut de Recerca de l'Hospital Universitari Vall d'Hebron, CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Imagine - Institut des maladies génétiques (IHU) (Imagine - U1163), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP), Institute of Child Health [London], University College of London [London] (UCL), People's Hospital of Peking University (PEKING - PHPU), Peking University [Beijing], Tallinn Children's Hospital [Tallinn, Estonia], Klinikverbund Kempten-Oberallgäu gGmbH, University of Shizuoka, Service de Génétique Clinique Chromosomique et Moléculaire, CHU Saint-Etienne-Hôpital Nord - Saint-Etienne, St. Sophia Children’s Hospital, Athens, Department of Clinical Genetics, St George’s University Hospitals, and Vrije Universiteit Brussel (VUB)
Subjects: calidad, acceso y evaluación de la atención sanitaria::calidad de la atención sanitaria::mecanismos de evaluación de la atención sanitaria::recopilación de datos::registros [ATENCIÓN DE SALUD], Pediatrics, [SDV]Life Sciences [q-bio], Disease, registry, GUIDELINES, RECOMMENDATIONS, lcsh:RC346-429, Tuberous sclerosis, Epilepsy, 0302 clinical medicine, Health care, Medicine and Health Sciences, 030212 general & internal medicine, TOSCA, TSC, management, rare diseases, resource use, Serveis sanitaris - Administració, Original Research, Esclerosi tuberosa, Settore MED/39, 3. Good health, medicine.anatomical_structure, Neurology, HEALTH-CARE UTILIZATION, BURDEN, Life Sciences & Biomedicine, Congenital, Hereditary, and Neonatal Diseases and Abnormalities::Congenital, Hereditary, and Neonatal Diseases and Abnormalities::Genetic Diseases, Inborn::Congenital, Hereditary, and Neonatal Diseases and Abnormalities::Genetic Diseases, Inborn::Neoplastic Syndromes, Hereditary::Tuberous Sclerosis [DISEASES], enfermedades y anomalías neonatales congénitas y hereditarias::enfermedades y anomalías neonatales congénitas y hereditarias::enfermedades genéticas congénitas::enfermedades y anomalías neonatales congénitas y hereditarias::enfermedades genéticas congénitas::síndromes neoplásicos hereditarios::esclerosis tuberosa [ENFERMEDADES], congenital, hereditary, and neonatal diseases and abnormalities, medicine.medical_specialty, Clinical Neurology, DIAGNOSIS, Bases de dades - Disseny, calidad, acceso y evaluación de la atención sanitaria::prestación sanitaria::recursos en salud [ATENCIÓN DE SALUD], 03 medical and health sciences, MANAGEMENT, medicine, Health Care Quality, Access, and Evaluation::Delivery of Health Care::Health Resources [HEALTH CARE], lcsh:Neurology. Diseases of the nervous system, Science & Technology, Subependymal giant cell astrocytoma, business.industry, Neurosciences, medicine.disease, LYMPHANGIOLEIOMYOMATOSIS, Lymphangioleiomyomatosis, Neurosciences & Neurology, GIANT-CELL ASTROCYTOMA, Neurology (clinical), TSC1, business, 030217 neurology & neurosurgery, Health Care Quality, Access, and Evaluation::Quality of Health Care::Health Care Evaluation Mechanisms::Data Collection::Records::Registries [HEALTH CARE], Rare disease
Abstract: TSC; Resource use; TOSCA TSC; Uso de recursos; TOSCA TSC; Ús de recursos; TOSCA Tuberous Sclerosis Complex (TSC) is a rare autosomal-dominant disorder caused by mutations in the TSC1 or TSC2 genes. Patients with TSC may suffer from a wide range of clinical manifestations; however, the burden of TSC and its impact on healthcare resources needed for its management remain unknown. Besides, the use of resources might vary across countries depending on the country-specific clinical practice. The aim of this paper is to describe the use of TSC-related resources and treatment paterns within the TOSCA registry. A total of 2,214 patients with TSC from 31 countries were enrolled and had a follow-up of up to 5 years. A search was conducted to identify the variables containing both medical and non-medical resource use information within TOSCA. This search was performed both at the level of the core project as well as at the level of the research projects on epilepsy, subependymal giant cell astrocytoma (SEGA), lymphangioleiomyomatosis (LAM), and renal angiomyolipoma (rAML) taking into account the timepoints of the study, age groups, and countries. Data from the quality of life (QoL) research project were analyzed by type of visit and age at enrollment. Treatments varied greatly depending on the clinical manifestation, timepoint in the study, and age groups. GAB Aergics were the most prescribed drugs for epilepsy, and mTOR inhibitors are dramatically replacing surgery in patients with SEGA, despite corrent recommendations proposing both treatment options.mTOR inhibitors are also becoming common treatments in rAML and LAMpatients. Forty-two out of the 143 patients (29.4%) who participated in the QoL research project reported inpatient stays over the last year. Data from non-medical resource use showed the critical impact of TSC on job status and capacity. Disability allowances were more common in children than adults (51.1% vs 38.2%). Psychological counseling, social services and social worker services were needed by
Published: 2019

9. Spatial variability of the relationships of runoff and sediment yield with weather types throughout the Mediterranean basin

Author: G. Desir, José María Senciales-González, Patricio Cid, Álvaro Gómez-Gutiérrez, Luis Merino-Martín, Carla Ferreira, Tíscar Espigares, Miguel A. Campo-Bescós, Albert Solé-Benet, Asunción Romero-Díaz, Julián Martínez-Fernández, Damien Raclot, Damià Vericat, Agata Novara, María Martínez-Mena, C. Le Bouteiller, Encarnación V. Taguas, Francesca Todisco, Antonio Canatário-Duarte, Luciano Mateos, Francesc Gallart, José Andrés López-Tarazón, Susana Bernal, Emmanouil A. Varouchakis, Y. Le Bissonnais, Mariano Moreno-de las Heras, Noemí Lana-Renault, A. Zabaleta, Dhais Peña-Angulo, M. Mercedes Taboada-Castro, Lea Wittenberg, Ramon J. Batalla, Vito Ferro, Susanne Schnabel, Xavier Úbeda, Victor Castillo, Matija Zorn, José Carlos González-Hidalgo, Ramón Bienes, Juan Albaladejo, M.T. Taboada-Castro, Feliciana Licciardello, C. Marín, V. Simonneaux, Óscar González-Pelayo, George P. Karatzas, H. Barhi, Roberto Lázaro, E. Roose, Jesús Rodrigo-Comino, Joan Estrany, Julián Campo, Elvira Díaz-Pereira, José Luis Rubio, Sébastien Klotz, Artemio Cerdà, Javier Casalí, Vicente Andreu, Helena Gómez-Macpherson, Nicolle Mathys, Yolanda Cantón, Estela Nadal-Romero, Teodoro Lasanta, Nicola Cortesi, Orestis Kairis, Rafael Giménez, Maria Jose Marques, María Fernández-Raga, A. Cheggour, José Damián Ruiz-Sinoga, Vincenzo Pampalone, José A. Gómez, Vincenzo Bagarello, Paloma Hueso-Gonzalez, José M. Nicolau, Ana Lucía, N. Moustakas, Costas Kosmas, M. L. Rodríguez-Blanco, Jérôme Latron, E. Gimeno, Juan F. Martínez-Murillo, Govern de les Illes Balears, Generalitat de Catalunya, Fundação para a Ciência e a Tecnologia (Portugal), Ministerio de Educación, Cultura y Deporte (España), Ministerio de Economía y Competitividad (España), European Commission, Ministerio de Ciencia, Innovación y Universidades (España), Universidad de Lleida, Ministerio de Educación y Ciencia (España), Instituto Universitario de Ciencias Ambientales (IUCA), Departamento de Geografía, University of Zaragoza, Instituto Pirenaico de Ecología (IPE), Soil and Water Conservation Research Group, Centro de Edafologia y Biologia Aplicada del Segura, Desertification Research Centre, Department of Environmental Quality and Soils, CIDE-CSIC, Department of Agricultural, Food and Forest Sciences, Università degli studi di Palermo - University of Palermo, Institut National de Recherche en Génie Rural, Eaux et Forêts de Tunisie (INRGREF), Catalan Institute for Water Research (ICRA), Faculty of Forest Sciences and Natural Resources, Universidad Austral de Chile, RIUS, Fluvial Dynamics Research Group, University of Lleida (UL), Integrative Freshwater Ecology Group, Centre d’Estudis Avançats de Blanes, Departamento Investigación Aplicada y Extensión Agraria, Instituto Madrileño de Investigación y Desarrollo Rural, Agrario y Alimentario (IMIDRA), ISFOOD Institute, Department of Projects and Rural Engineering, Universidad Pública de Navarra, School of Agriculture, Food and Rural Development, Center for GeoBioSciences, GeoTechnologies and GeoEngineering (GEOBIOTEC), Universidade da Beira Interior, Department of Agronomy (Soil Science Area), University of Almeria, Department of Geography, Soil Erosion and Degradation Research Group, Université de Valence, Laboratorio Nacional de Genómica para la Biodiversidad (LANGEBIO), Maize Genetics and Genomics Lab, CINVESTAV, Department of Earth Sciences, Centro Nacional de Supercomputación, Departamento de Ciencias de la Tierra, Departamento de Ciencias de la Vida, Unidad de Ecología, Universidad de Alcalá de Henares (UAH), Department of Geography, Mediterranean Ecogeomorphological and Hydrological Connectivity Research Team (MEDhyCON), University of the Balearic Islands (UIB), Department of Applied Chemistry and Physics, University of Leon, College of Agriculture, Research Centre for Natural Resources, Environment and Society (CERNAS), Instituto Politécnico de Coimbra, Department of Earth and Marine Sciences [Palermo], Institute of Environmental Assessment and Water Research (IDAEA), Consejo Superior de Investigaciones Científicas [Spain] (CSIC), Instituto de Agricultura Sostenible (IAS), Instituto de Agricultura, INTERRA Research Institute, University of Extremadura, Department of Environment and Planning, Earth Surface Processes Team (ESP) Centre for Environmental and Marine Studies (CESAM), University of Aveiro, Department of Natural Resources Management and Agricultural Engineering, Agricultural University of Athens, School of Environmental Engineering, Technical University of Crete, Erosion torrentielle neige et avalanches (UR ETGR (ETNA)), Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA), Université Grenoble Alpes (COMUE) (UGA), Area of Physical Geography, DCH, Universidad de la Rioja, Experimental Station of Arid Zones (EEZA), Consejo Superior de Investigaciones Científicas, Laboratoire d'étude des Interactions Sol - Agrosystème - Hydrosystème (UMR LISAH), Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut de Recherche pour le Développement (IRD [ Madagascar])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Department of Agriculture, Food and Environment, Department of Geography, Mediterranean Ecogeomorphological and Hydrological Connectivity Research Team (MEDhyCON) Hydrol Connect Res, Dept Geog, Palma De Mallorca 07122, Spain, Institute of Earth and Environmental Science [Potsdam], University of Potsdam, Faculty of Sciences, Center for Applied Geosciences, Eberhard Karls University Tübingen, Instituto Hispano Luso de Investigaciones Agrarias, Universidad de Salamanca, Botanique et Modélisation de l'Architecture des Plantes et des Végétations (UMR AMAP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Institut d'Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS), Université Paul Valéry (Montpellier 3), Université de Montpellier (UM), École pratique des hautes études (EPHE), Institut de Recherche pour le Développement (IRD [Bolivie]), Centre d’Ecologie Fonctionnelle et Evolutive (CEFE), Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-École pratique des hautes études (EPHE)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université Paul-Valéry - Montpellier 3 (UM3), Instituto Universitario de Ciencias Ambientales, Escuela Politécnica Superior, Faculty of Sciences, Centre for Advanced Scientific Research (CICA), University of A Coruña (UDC), Physical Geography, Trier University, Departamento de Geografía, Universidad de Cantabria [Santander], UMR Eco&Sols, Centre d'études spatiales de la biosphère (CESBIO), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Observatoire Midi-Pyrénées (OMP), Université Fédérale Toulouse Midi-Pyrénées-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées, ETSIAM Campus Rabanales, Universidad de Cordoba, Department of Agriculture-Food and Environmental Sciences, Agricultural and Biosystems Engineering Research Unit, Università degli Studi di Perugia (UNIPG), Departamento de Geografía, Grup de Recerca Ambiental Mediterrània, University of Barcelona, Forest Science and Technology Centre of Catalonia, Department of Geography and Environmental Studies, University of Nairobi (UoN), Science and Technology Faculty, Hydro-Environmental Processes Research Group, University of the Basque Country (University of the Basque Country), Geographical Institute, Scientific Research Center of the Slovenian Academy of Sciences and Arts (ZRC SAZU), MINECO-FEDER [CGL2014-52135-C3-3-R, CGL2014-59946-R, CGL2015-65569-R, CGL2015-64284-C2-2-R, PCIN-2017-061/AEI], Spanish Ministry of Economy and Competitiveness, MEC [RYC-2013-14371, RYC-2010-06264], Spanish Ministry of Education, Culture and Sports, Portuguese Science and Technology Foundation [SFRH/BPD/120093/2016], Juan de la Cierva postdoctoral contract from the MEC [IJCI-2015-26463], Vicenc Mut postdoctoral fellowship from the Autonomous Balearic Government [CAIB PD/038/2016], Secretariat for Universities and Research of the Department of the Economy and Knowledge of the Autonomous Government of Catalonia [2014 SGR 645], CERCA Programme, COST action CONNECTEUR [ES1306], University of Zaragoza - Universidad de Zaragoza [Zaragoza], Instituto Pirenaico de Ecologìa = Pyrenean Institute of Ecology [Zaragoza] (IPE - CSIC), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Institut National de Recherche en Génie Rural Eaux et Forêts (INRGREF), Ecole Nationale du Génie Rural, des Eaux et des Forêts (ENGREF)-Institution de la Recherche et de l'Enseignement Supérieur Agricoles [Tunis] (IRESA), Instituto Catalán de Investigación del Agua - ICRA (SPAIN) (ICRA), Universidad Pública de Navarra [Espagne] = Public University of Navarra (UPNA), Universidade de Aveiro, Universitat de València (UV), Université Cadi Ayyad [Marrakech] (UCA), Barcelona Supercomputing Center - Centro Nacional de Supercomputacion (BSC - CNS), Instituto de Agricultura Sostenible - Institute for Sustainable Agriculture (IAS CSIC), Universidad de Extremadura - University of Extremadura (UEX), Universidad de Málaga [Málaga] = University of Málaga [Málaga], Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), University of Potsdam = Universität Potsdam, Eberhard Karls Universität Tübingen = Eberhard Karls University of Tuebingen, Universidad Autónoma de Madrid (UAM), Université Paul-Valéry - Montpellier 3 (UPVM), École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL), Université Paul-Valéry - Montpellier 3 (UPVM)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Ecologie fonctionnelle et biogéochimie des sols et des agro-écosystèmes (UMR Eco&Sols), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire Midi-Pyrénées (OMP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Météo-France -Institut national des sciences de l'Univers (INSU - CNRS)-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Météo-France -Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université de Toulouse (UT), Universidad de Córdoba = University of Córdoba [Córdoba], Università degli Studi di Perugia = University of Perugia (UNIPG), Centre de Ciència i Tecnologia Forestal de Catalunya (CTFC), Pena-Angulo D., Nadal-Romero E., Gonzalez-Hidalgo J.C., Albaladejo J., Andreu V., Bagarello V., Barhi H., Batalla R.J., Bernal S., Bienes R., Campo J., Campo-Bescos M.A., Canatario-Duarte A., Canton Y., Casali J., Castillo V., Cerda A., Cheggour A., Cid P., Cortesi N., Desir G., Diaz-Pereira E., Espigares T., Estrany J., Fernandez-Raga M., Ferreira C.S.S., Ferro V., Gallart F., Gimenez R., Gimeno E., Gomez J.A., Gomez-Gutierrez A., Gomez-Macpherson H., Gonzalez-Pelayo O., Hueso-Gonzalez P., Kairis O., Karatzas G.P., Klotz S., Kosmas C., Lana-Renault N., Lasanta T., Latron J., Lazaro R., Le Bissonnais Y., Le Bouteiller C., Licciardello F., Lopez-Tarazon J.A., Lucia A., Marin C., Marques M.J., Martinez-Fernandez J., Martinez-Mena M., Martinez-Murillo J.F., Mateos L., Mathys N., Merino-Martin L., Moreno-de las Heras M., Moustakas N., Nicolau J.M., Novara A., Pampalone V., Raclot D., Rodriguez-Blanco M.L., Rodrigo-Comino J., Romero-Diaz A., Roose E., Rubio J.L., Ruiz-Sinoga J.D., Schnabel S., Senciales-Gonzalez J.M., Simonneaux V., Sole-Benet A., Taguas E.V., Taboada-Castro M.M., Taboada-Castro M.T., Todisco F., Ubeda X., Varouchakis E.A., Vericat D., Wittenberg L., Zabaleta A., Zorn M., Instituto Pirenaico de Ecologia (IPE), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Universidad Autonoma de Madrid (UAM), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD [France-Sud]), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Université Paul-Valéry - Montpellier 3 (UPVM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut de Recherche pour le Développement (IRD [France-Sud]), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire Midi-Pyrénées (OMP), and Météo France-Centre National d'Études Spatiales [Toulouse] (CNES)-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Météo France-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)
Subjects: 010504 meteorology & atmospheric sciences, Runoff, Erosion, Mediterranean basin, Sediment yield, Synoptic weather types, 0207 environmental engineering, [SDU.STU]Sciences of the Universe [physics]/Earth Sciences, 02 engineering and technology, 01 natural sciences, Mediterranean Basin, Hydrology (agriculture), Erosão, Produção de sedimentos, Escoamento, Bacia Mediterrânica, Soil retrogression and degradation, ddc:550, 020701 environmental engineering, 0105 earth and related environmental sciences, Water Science and Technology, Hydrology, Sediment, Institut für Umweltwissenschaften und Geographie, 15. Life on land, 6. Clean water, 13. Climate action, Environmental science, Spatial variability, Soil conservation, Surface runoff
Abstract: Este artículo contiene 16 páginas, 6 figuras, 2 tablas., Soil degradation by water is a serious environmental problem worldwide, with specific climatic factors being the major causes. We investigated the relationships between synoptic atmospheric patterns (i.e. weather types, WTs) and runoff, erosion and sediment yield throughout the Mediterranean basin by analyzing a large database of natural rainfall events at 68 research sites in 9 countries. Principal Component Analysis (PCA) was used to identify spatial relationships of the different WTs including three hydro-sedimentary variables: rainfall, runoff, and sediment yield (SY, used to refer to both soil erosion measured at plot scale and sediment yield registered at catchment scale). The results indicated 4 spatial classes of rainfall and runoff: (a) northern sites dependent on North (N) and North West (NW) flows; (b) eastern sites dependent on E and NE flows; (c) southern sites dependent on S and SE flows; and, finally, (d) western sites dependent on W and SW flows. Conversely, three spatial classes are identified for SY characterized by: (a) N and NE flows in northern sites (b) E flows in eastern sites, and (c) Wand SW flows in western sites. Most of the rainfall, runoff and SY occurred during a small number of daily events, and just a few WTs accounted for large percentages of the total. Our results confirm that characterization by WT improves understanding of the general conditions under which runoff and SY occur, and provides useful information for understanding the spatial variability of runoff, and SY throughout the Mediterranean basin. The approach used here could be useful to aid of the design of regional water management and soil conservation measures., This research was supported by projects funded by the MINECOFEDER: CGL2014-52135-C3-3-R, CGL2014-59946-R, CGL2015-65569- R, CGL2015-64284-C2-2-R, and PCIN-2017-061/AEI. When this manuscript was first submitted Estela Nadal-Romero and Damià Vericat received a “Ramón y Cajal” postdoctoral contract (RYC-2013-14371 and RYC‐2010‐06264, Spanish Ministry of Economy and Competitiveness, MEC). Damià Vericat is now a Serra Húnter Fellow at the University of Lleida. María Fernández-Raga received a “José Castillejo” postdoctoral grant (Spanish Ministry of Education, Culture and Sports). Carla Ferreira was supported by a post-doctoral research grant from the Portuguese Science and Technology Foundation (SFRH/ BPD/120093/2016). Mariano Moreno-de las Heras received a Juan de la Cierva postdoctoral contract (IJCI-2015-26463) from the MEC. José Andrés López-Tarazón received a Vicenç Mut postdoctoral fellowship from the Autonomous Balearic Government (CAIB PD/038/2016). José Andrés López-Tarazón and Ramon Batalla also acknowledge the Secretariat for Universities and Research of the Department of the Economy and Knowledge of the Autonomous Government of Catalonia for supporting the Consolidated Research Group 2014 SGR 645 (RIUSFluvial Dynamics Research Group), and the CERCA Programme. This paper has benefited from the Lab and Field Data Pool created within the framework of the COST action CONNECTEUR (ES1306).
Published: 2019

10. A novel antioxidant formulation designed to treat male infertility associated with oxidative stress: promising preclinical evidence from animal models

Author: Drevet, Joël, Gharagozloo, P., Gutierrez-Adán, A., Champroux, A., Noblanc, A., Kocer, A., Calle, A., Pérez-Cerezales, S., Pericuesta, E., Polhemus, A., Moazamian, A., Drevet, J.R., Aitken, R.J., Génétique, Reproduction et Développement (GReD), Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de la Santé et de la Recherche Médicale (INSERM), Departamento de Reproduccion Animal y Conservacion de Recursos Zoogeneticos, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria = National Institute for Agricultural and Food Research and Technology (INIA), Biologie et Pathologie des Communications Cellulaires Dans les Glandes Endocrines, Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Department of Molecular Biology, Faculty of Biology, Campus Vegazana s/n, University of Leon, and Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS)
Subjects: Male, 0301 basic medicine, Pregnancy Rate, Glutathione peroxidase 5, medicine.disease_cause, Antioxidants, Sperm DNA damage, Male infertility, Mice, 0302 clinical medicine, Pregnancy, ComputingMilieux_MISCELLANEOUS, [SDV.BDD.GAM]Life Sciences [q-bio]/Development Biology/Gametogenesis, Mice, Knockout, education.field_of_study, 030219 obstetrics & reproductive medicine, medicine.diagnostic_test, Rehabilitation, Pregnancy Outcome, Obstetrics and Gynecology, Spermatozoa, 3. Good health, [SDV.IMM.IA]Life Sciences [q-bio]/Immunology/Adaptive immunology, 8-Hydroxy-2'-Deoxyguanosine, Models, Animal, Female, Infertility, medicine.medical_specialty, DNA damage, Population, Semen, Semen analysis, Biology, [SDV.BDLR.RS]Life Sciences [q-bio]/Reproductive Biology/Sexual reproduction, Andrology, 03 medical and health sciences, Internal medicine, medicine, Animals, education, Infertility, Male, Glutathione Peroxidase, Deoxyguanosine, medicine.disease, Sperm, Mice, Inbred C57BL, Semen Analysis, Oxidative Stress, 030104 developmental biology, Endocrinology, Reproductive Medicine, Reactive oxygen species, Biomarkers, Oxidative stress, Scrotal heat stress, DNA Damage
Abstract: STUDY QUESTION Does a novel antioxidant formulation designed to restore redox balance within the male reproductive tract, reduce sperm DNA damage and increase pregnancy rates in mouse models of sperm oxidative stress? SUMMARY ANSWER Oral administration of a novel antioxidant formulation significantly reduced sperm DNA damage in glutathione peroxidase 5 (GPX5), knockout mice and restored pregnancy rates to near-normal levels in mice subjected to scrotal heat stress. WHAT IS KNOWN ALREADY Animal and human studies have documented the adverse effect of sperm DNA damage on fertilization rates, embryo quality, miscarriage rates and the transfer of de novo mutations to offspring. Semen samples of infertile men are known to be deficient in several key antioxidants relative to their fertile counterparts. Antioxidants alone or in combination have demonstrated limited efficacy against sperm oxidative stress and DNA damage in numerous human clinical trials, however these studies have not been definitive and an optimum combination has remained elusive. STUDY DESIGN, SIZE, DURATION The efficacy of the antioxidant formulation was evaluated in two well-established mouse models of oxidative stress, scrotal heating and Gpx5 knockout (KO) mice, (n = 12 per experimental group), by two independent laboratories. Mice were provided the antioxidant product in their drinking water for 2-8 weeks and compared with control groups for sperm DNA damage and pregnancy rates. PARTICIPANTS/MATERIALS, SETTING, METHODS In the Gpx5 KO model, oxidative DNA damage was monitored in spermatozoa by immunocytochemical detection of 8-hydroxy-2′-deoxyguanosine (8OHdG). In the scrotal heat stress model, male fertility was tested by partnering with three females for 5 days. The percentage of pregnant females, number of vaginal plugs, resorptions per litter, and litter size were recorded. MAIN RESULTS AND ROLE OF CHANCE Using immunocytochemical detection of 8OHdG as a biomarker of DNA oxidation, analysis of control mice revealed that around 30% of the sperm population was positively stained. This level increased to about 60% in transgenic mice deficient in the antioxidant enzyme, GPX5. Our results indicate that an 8 week pretreatment of Gpx5 KO mice with the antioxidant formulation provided complete protection of sperm DNA against oxidative damage. In mouse models of scrotal heat stress, only 35% (19/54) of female mice became pregnant resulting in 169 fetuses with 18% fetal resorption (30/169). This is in contrast to the antioxidant pretreated group where 74% (42/57) of female mice became pregnant, resulting in 427 fetuses with 9% fetal resorption (38/427). In both animal models the protection provided by the novel antioxidant was statistically significant (P < 0.01 for the reduction of 8OHdG in the spermatozoa of Gpx5 KO mice and P < 0.05 for increase in fertility in the scrotal heat stress model). LIMITATIONS, REASONS FOR CAUTION It was not possible to determine the exact level of antioxidant consumption for each mouse during the treatment period. WIDER IMPLICATIONS OF THE FINDINGS Recent clinical studies confirm moderate to severe sperm DNA damage in about 60% of all men visiting IVF centers and in about 80% of men diagnosed with idiopathic male infertility. Our results, if confirmed in humans, will impact clinical fertility practice because they support the concept of using an efficacious antioxidant supplementation as a preconception therapy, in order to optimize fertilization rates, help to maintain a healthy pregnancy and limit the mutational load carried by children. STUDY FUNDING/COMPETING INTEREST(S) The study was funded by the Clermont Universite and the University of Madrid. P.G. is the Managing Director of CellOxess LLC, which has a commercial interest in the detection and resolution of oxidative stress. A.M.;A.P. are employees of CellOxess, LLC. J.R.D.;A.G.-A.;R.J.A. are honorary members of the CellOxess advisory board. © 2016 The Author 2016. Published by Oxford University Press on behalf of the European Society of Human Reproduction a d Embryology. All rights reserved.
Published: 2016

11. Variant discovery in the sheep milk transcriptome using RNA sequencing

Author: Juan José Arranz, Aroa Suárez-Vega, Beatriz Gutiérrez-Gil, Gwenola Tosser-Klopp, Christophe Klopp, Fac Vet, Dept Anim Prod, University of Leon, Unité de Mathématiques et Informatique Appliquées de Toulouse (MIAT INRA), Institut National de la Recherche Agronomique (INRA), Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), and Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-École nationale supérieure agronomique de Toulouse [ENSAT]
Subjects: 0301 basic medicine, Candidate gene, [SDV]Life Sciences [q-bio], Quantitative Trait Loci, RNA-Seq, Single-nucleotide polymorphism, Biology, Quantitative trait locus, dairy sheep, Transcriptome, 03 medical and health sciences, Genetic variation, Genetics, Animals, Sheep milk, Gene, milk somatic cell, 2. Zero hunger, transcriptome variant, Sheep, Sequence Analysis, RNA, Milk Somatic Cells, Gene Expression Profiling, 0402 animal and dairy science, Genetic Variation, Molecular Sequence Annotation, 04 agricultural and veterinary sciences, 040201 dairy & animal science, Dairying, rna-seq, 030104 developmental biology, Milk, Transcriptome Variants, Female, Research Article, Biotechnology
Abstract: Background The identification of genetic variation underlying desired phenotypes is one of the main challenges of current livestock genetic research. High-throughput transcriptome sequencing (RNA-Seq) offers new opportunities for the detection of transcriptome variants (SNPs and short indels) in different tissues and species. In this study, we used RNA-Seq on Milk Sheep Somatic Cells (MSCs) with the goal of characterizing the genetic variation within the coding regions of the milk transcriptome in Churra and Assaf sheep, two common dairy sheep breeds farmed in Spain. Results A total of 216,637 variants were detected in the MSCs transcriptome of the eight ewes analyzed. Among them, a total of 57,795 variants were detected in the regions harboring Quantitative Trait Loci (QTL) for milk yield, protein percentage and fat percentage, of which 21.44% were novel variants. Among the total variants detected, 561 (2.52%) and 1,649 (7.42%) were predicted to produce high or moderate impact changes in the corresponding transcriptional unit, respectively. In the functional enrichment analysis of the genes positioned within selected QTL regions harboring novel relevant functional variants (high and moderate impact), the KEGG pathway with the highest enrichment was “protein processing in endoplasmic reticulum”. Additionally, a total of 504 and 1,063 variants were identified in the genes encoding principal milk proteins and molecules involved in the lipid metabolism, respectively. Of these variants, 20 mutations were found to have putative relevant effects on the encoded proteins. Conclusions We present herein the first transcriptomic approach aimed at identifying genetic variants of the genes expressed in the lactating mammary gland of sheep. Through the transcriptome analysis of variability within regions harboring QTL for milk yield, protein percentage and fat percentage, we have found several pathways and genes that harbor mutations that could affect dairy production traits. Moreover, remarkable variants were also found in candidate genes coding for major milk proteins and proteins related to milk fat metabolism. Several of the SNPs found in this study could be included as suitable markers in genotyping platforms or custom SNP arrays to perform association analyses in commercial populations and apply genomic selection protocols in the dairy production industry. Electronic supplementary material The online version of this article (doi:10.1186/s12864-017-3581-1) contains supplementary material, which is available to authorized users.
Published: 2017

12. Editorial: About the Foodborne Pathogen Campylobacter

Author: Avelino Alvarez-Ordóñez, Ian F. Connerton, Odile Tresse, UMR 1014 SECurité des ALIments et Microbiologie, Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Institute of Food Science and Technology, University of Leon, Department of Food Hygiene and Technology, University of Nottingham, UK (UON), and Tresse, Odile
Subjects: 0301 basic medicine, Microbiology (medical), Foodborne pathogen, foodborne pathogen, Campylobacter, [SDV]Life Sciences [q-bio], 030106 microbiology, lcsh:QR1-502, methodology, Biology, control strategies, medicine.disease_cause, metabolism and regulation, chicken gut microbiota, Microbiology, lcsh:Microbiology, 03 medical and health sciences, 030104 developmental biology, medicine, host interaction, epidemiology, ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2017

13. Molecular basis of spermatogenesis and sperm quality

Author: Martin Pšenička, Catherine Labbé, Elsa Cabrita, Paz Herraez, Marta F. Riesco, David G. Valcarce, Vanesa Robles, Promontorio de San Martín s/n, Instituto Español de Oceanografía, Department of Molecular Biology, Eberhard Karls Universität Tübingen, INDEGSAL, University of Leon, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Centre of Marine Sciences [Faro] (CCMAR), University of Algarve [Portugal], Faculty of Fisheries and Protection of Waters, University of South Bohemia, MINECO AGL2015-68330- C2-1-R as well as COST Office (Food and Agriculture COST Action FA1205: AQUAGAMETE), Fundación Ramón Areces, Ministerio de Ciencia e Innovación MICINN AGL2009-06994, AGL2014-53167-C3-3-R, Ramón y Cajal Program RYC- 2008-02339, Junta de Castilla y León E-24-2009-0036681 EDU/1084/2012, Fondo Social Europeo, the Ministry of Education, Youth and Sports of the Czech Republic — projects CENAKVA (No. CZ.1.05/2.1.00/01.0024) and CENAKVA II (No. LO1205 under the NPU I program), the Czech Science Foundation (No. P502/13/26952S), and French CRB Anim project «Investissements d’avenir», ANR-11-INBS-0003, Eberhard Karls Universität Tübingen = Eberhard Karls University of Tuebingen, Faculty of Fisheries and Protection of Waters [University of South Bohemia], MINECO AGL2015-68330- C2-1-R as well as COST Office (Food and Agriculture COST Action FA1205: AQUAGAMETE), Fundación Ramón Areces, Ministerio de Ciencia e Innovación MICINN AGL2009-06994, AGL2014-53167-C3-3-R, Ramón y Cajal Program RYC- 2008-02339, Junta de Castilla y León E-24-2009-0036681 EDU/1084/2012, Fondo Social Europeo, the Ministry of Education, Youth and Sports of the Czech Republic — projects CENAKVA (No. CZ.1.05/2.1.00/01.0024) and CENAKVA II (No. LO1205 under the NPU I program), the Czech Science Foundation (No. P502/13/26952S), and French CRB Anim project «Investissements d’avenir», ANR-11-INBS-0003., ANR-11-INBS-0003,CRB-Anim,Réseau de Centres de Ressources Biologiques pour les animaux domestiques(2011), European Project: COST Action FA 1205, AQUAGAMETE, and Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Recherche Agronomique (INRA)
Subjects: Male, 0301 basic medicine, germ cells, sperm quality, Centro Oceanográfico de Santander, [SDV]Life Sciences [q-bio], Expression, dna, Germline, Non-coding RNAs, Epigenesis, Genetic, chemistry.chemical_compound, Endocrinology, poisson, Early embryo development, salmonids, Bisphenol-A exposure, Zebrafish, Genetics, salmonidae, trout, oncorhynchus mykiss, mrna, Fishes, adn, mammifère, Spermatozoa, rainbow trout, Cell biology, Messenger-rna, medicine.anatomical_structure, Epigenetics, Germ cell, endocrine system, Acuicultura, Sperm quality, Offspring, mRNAs, spermatogenèse, Biology, Dna-damage, 03 medical and health sciences, épigénétique, rainbow, medicine, Germ cells, Animals, Non coding RNAs, Germ, mammals, Spermatogenesis, Chromatoid body, fish, epigenetics, RNA, qualité du sperme, DNA, Micrornas, 030104 developmental biology, chemistry, cellule germinale, Male germ-cells, Animal Science and Zoology, germ-line cells, truite arc en ciel
Abstract: Spermatozoan quality can be evaluated in different ways, here we focus on the analysis of DNA, RNA and epigenetic status of germ cells. These characterizations also can be the bases for explaining sperm quality at other levels, so we will see how some of these molecules could affect other sperm quality markers. Moreover, we consider the possibility of using some of these molecules as predictors of sperm quality in terms of the ability to produce healthy offspring. The relevant effect of different types of RNA molecules in germ line specification and spermatogenesis and the importance of germ cell DNA integrity and a proper epigenetic pattern will be also discussed. Although most studies at this level have been performed in mammals, some information is available for fish; these recent discoveries in fish models are included. We provide a general overview on how these molecules could have a deep influence in the final sperm quality., SI
Published: 2016

14. Epigenetics in fish gametesand early embryo

Author: Vanesa Robles, Catherine Labbé, María Paz Herráez, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Instituto Español de Oceanografía, Department of Molecular Biology, University of Leon, Spanish Ministry of Economy and Competitiveness AGL2011-27787, AGL2015-68330-C2-1-R, French CRB Anim. project «Investissements d’avenir», ANR-11-INBS-0003. Support of the EU COST Actions FA1205: AQUAGAMETE and FA 1201: EPICONCEPT, Esther Lubzens, Catherine Labbé, Andrzej Ciereszko, ANR-11-INBS-0003,CRB-Anim,Réseau de Centres de Ressources Biologiques pour les animaux domestiques(2011), European Project: COST Action FA 1205, AQUAGAMETE, European Project: COST Action FA1201,EPICONCEPT, and Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Recherche Agronomique (INRA)
Subjects: 0301 basic medicine, Oocyte, Centro Oceanográfico de Santander, Acuicultura, Cellular differentiation, [SDV]Life Sciences [q-bio], embryo, Reproductive technology, Aquatic Science, Biology, Development, sperm, Gametogenesis, gametogenesis, 03 medical and health sciences, medicine, 14. Life underwater, Epigenetics, oocyte, development, Organism, Genetics, Epigenetic, Embryo, reproductive biotechnology, Sperm, 030104 developmental biology, medicine.anatomical_structure, Evolutionary biology, Reproductive biotechnology, Gamete, Reprogramming, epigenetic
Abstract: The spermatozoa and oocyte are very specialized cells which possess the unique ability to fuse and to produce an embryo. This embryo will develop into a mature organism capable of producing gametes again. This colloquial statement comprehends however the most complex mechanisms in biology, namely, cell differentiation and cell reprogramming. Epigenetic modifications are major actors in this process, both during gametogenesis and embryo development. The epigenetic concept is resulting from the growing scientific understanding of embryology and genetics, and it is made operational by generic molecular mechanisms, namely DNA methylation, histone tails modifications, and non-coding RNAs. Epigenetic modifications associated with gamete differentiation and early embryo development are quite well described in mammals, but increasing research is developed in fish today, either in model or in aquaculture species. Because fish are living organisms thoroughly exposed to temperature and to water quality, environment is likely to influence the epigenetic pattern of the gametes, embryo, or adult organisms. Reproductive biotechnologies are also at risk to modify gametes and embryo epigenetics. This review is aimed towards giving the basic knowledge on the molecular actors of epigenetics, highlighting the developing knowledge in mammals which can be useful to fish research and proposing the possible area where epigenetics can enrich research on fish gametes and reproductive technologies., SI
Published: 2016

15. Editorial: biofilms from a food microbiology perspective: structures, functions, and control strategies

Author: Romain Briandet, Avelino Alvarez-Ordóñez, MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Department of Food Hygiene and Technology, University of Leon, Institute of Food Science and Technology, and Álvarez-Ordóñez, Avelino
Subjects: 0301 basic medicine, Microbiology (medical), biofilms, bioprotection, control, food quality, food safety, structure, [SDV]Life Sciences [q-bio], 030106 microbiology, Biology, Microbiology, 03 medical and health sciences, Food microbiology, ComputingMilieux_MISCELLANEOUS, 2. Zero hunger, business.industry, Perspective (graphical), Food safety, 6. Clean water, Biotechnology, 13. Climate action, Engineering ethics, business, Food quality
Abstract: [début du texte ] Materials and equipment in food processing industries are colonized by surface-associated microbial communities called biofilms. In these biostructures microorganisms are embedded in a complex organic matrix composed essentially of polysaccharides, nucleic acids, and proteins. This organic shield contributes to the mechanical biofilm cohesion and triggers tolerance to environmental stresses such as dehydration or nutrient deprivation. Notably, cells within a biofilm are more tolerant to sanitation processes and the action of antimicrobial agents than their free living (or planktonic) counterparts. Such properties make conventional cleaning and disinfection protocols normally not effective in eradicating these biocontaminants. Biofilms are thus a continuous source of persistent microorganisms, including spoilage and pathogenic microorganisms, leading to repeated contamination of processed food with important economic and safety impact. Alternatively, in some particular settings, biofilm formation by resident or technological microorganisms can be desirable, due to possible enhancement of food fermentations or as a means of bioprotection against the settlement of pathogenic microorganisms.
Published: 2016

16. Cryobanking of aquatic species

Author: Terrence R. Tiersch, Marc Suquet, Paz Herraez, Ana Tereza de Mendonça Viveiros, Vanesa Robles, Ákos Horváth, S. Martínez-Páramo, Catherine Labbé, Serean L. Adams, Tiantian Zhang, Elsa Cabrita, Centre of Marine Sciences [Faro] (CCMAR), University of Algarve [Portugal], Szent István University, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Bournemouth University [Poole] (BU), Spanish Oceanographic Institute, Universidad de León [León], Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Cawthron Institute, Universidade Federal de Lavras = Federal University of Lavras (UFLA), Louisiana State University (LSU), COST Office (Food and Agriculture COST Action FA1205: AQUAGAMETE) and projects KLING 31-03-05 - FEP-73, CRIOBIV 31-03-05 - FEP-59, REPLING 31-03-05 - FEP-69 financed by Portuguese PROMAR program, grants AGL2011 - 27787 and AGL2014 - 53167-C3-3-R from the Spanish Ministry of Economy and Competitiveness, NKFIH grant K109847, 9878/2015/FEKUT of the Ministry of Human Resources of Hungary awarded to Szent István University, French program 'Investissements d'Avenir' ANR-11 - INBS-0003 (CRB-Anim 2013-2019), and Creche (Ofimer 136/08/C) and CRB anim (ANR-11 - INBS-0003), National Institutes of Health of U.S. (5R24OD010441), New Zealand Ministry of Business, Innovation and Employment (CAWX1315), Brazilian funding agencies FAP EMIG and CNPq, and FCT postdoctoral fellowship (SFRH/BPD/48520/2008) funded by Portuguese National Funds (MEC), Esther Lubzens, Catherine Labbé, Andrzej Ciereszko, ANR-11-INBS-0003,CRB-Anim,Réseau de Centres de Ressources Biologiques pour les animaux domestiques(2011), European Project: COST Action FA 1205, AQUAGAMETE, Department of Aquaculture, Swedish University of Agricultural Sciences (SLU), Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Recherche Agronomique (INRA), Poole House, Talbot Campus, Bournemouth University, Santander Oceanographic Centre, El Bocal, Barrio Corbanera s/n Bocal, Instituto Español de Oceanografía, Department of Molecular Biology and INDEGSAL, University of Leon, Institut de Recherche pour le Développement (IRD)-Institut Universitaire Européen de la Mer (IUEM), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Université de Brest (UBO)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS), Private Bag 2, Nelson, New Zealand 7042. AgResearch, Private Bag 3123, Department of Animal Sciences, University of Illinois System, School of Renewable Natural Resources, French program 'Investissements d'Avenir' ANR-11 - INBS-0003 (CRB-Anim 2013-2019), and Creche (Ofimer 136/08/C) and CRB anim (AN-11 - INBS-0003), Institut de Recherche pour le Développement (IRD)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Federal University of Lavras, Institut Universitaire Européen de la Mer (IUEM), and Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Brest (UBO)
Subjects: 0301 basic medicine, Germplasm, Centro Oceanográfico de Santander, cryobanque, Range (biology), [SDV]Life Sciences [q-bio], gamète, Broodstock, Aquaculture, poisson, Grayling Thymallus-Thymallus, Primordial germ-cells, Hippoglossus-Hippoglossus L, brésil, Ecology, Marine fish, Fish gametes, germplasm, Mussel Mytilus-Galloprovincialis, Paralichthys-Olivaceus Embryos, nouvelle zélande, europe, High-throughput cryopreservation, Acuicultura, australie, Aquatic Science, Biology, Zebrafish Danio-Rerio, Article, 03 medical and health sciences, Aquatic species, Cryobanking, Oyster Crassostrea-Gigas, 14. Life underwater, espèce aquacole, fish gametes, Invertebrate, business.industry, cryoconservation du sperme, Carp Cyprinus-Carpio, ACL, Aquatic animals, cryobanking, matériel génétique, Aquatic animal, états-unis, cryoconservation, Trout Oncorhynchus-Mykiss, 030104 developmental biology, aquatic animals, cryofixation, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, business, seed collections, usa
Abstract: This review is focused on the applications of genome cryobanking of aquatic species including freshwater and marine fish, as well as invertebrates. It also reviews the latest advances in cryobanking of model species, widely used by the scientific community worldwide, because of their applications in several fields. The state of the art of cryopreservation of different cellular types (sperm, oocytes, embryos, somatic cells and primordial germ cells or early spermatogonia) is discussed focusing on the advantages and disadvantages of each procedure according to different applications. A special review on the need of standardization of protocols has also been carried out. In summary, this comprehensive review provides information on the practical details of applications of genome cryobanking in a range of aquatic species worldwide, including the cryobanks established in Europe, USA, Brazil, Australia and New Zealand, the species and type of cells that constitute these banks and the utilization of the samples preserved. Statement of relevance This review compiles the last advances on germplasm cryobanking of freshwater and marine fish species and invertebrates, with high value for commercial aquaculture or conservation. It is reviewed the most promising cryopreservation protocols for different cell types, embryos and larvae that could be applied in programs for genetic improvement, broodstock management or conservation of stocks to guarantee culture production., SI
Published: 2016

17. Basic Oxygen Furnace steel slag aggregates for phosphorus treatment. Evaluation of its potential use as a substrate in constructed wetlands

Author: Ivan Blanco, Luis E. Sáenz de Miera, Gemma Ansola, Pascal Molle, UNIVERSITY OF LEON ESP, Partenaires IRSTEA, Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA), Milieux aquatiques, écologie et pollutions (UR MALY), and Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)
Subjects: Basic oxygen steelmaking, Environmental Engineering, chemistry.chemical_element, Industrial Waste, Portable water purification, 02 engineering and technology, 010501 environmental sciences, 01 natural sciences, Waste Disposal, Fluid, Water Purification, chemistry.chemical_compound, Waste Management and Disposal, Dissolution, 0105 earth and related environmental sciences, Water Science and Technology, Civil and Structural Engineering, Precipitation (chemistry), Ecological Modeling, Phosphorus, Metallurgy, Slag, 021001 nanoscience & nanotechnology, Phosphate, Pollution, 6. Clean water, chemistry, Spain, Steel, visual_art, Wetlands, [SDE]Environmental Sciences, visual_art.visual_art_medium, Calcium, 0210 nano-technology, Saturation (chemistry), Water Pollutants, Chemical, Nuclear chemistry
Abstract: International audience; Basic Oxygen Furnace (BOF) steel slag aggregates from NW Spain were tested in batch and column experiments to evaluate its potential use as a substrate in constructed wetlands (CWs). The objectives of this study were to identify the main P removal mechanisms of BOF steel slag and determine its P removal capacity. Also, the results were used to discuss the suitability of this material as a substrate to be used in CWs. Batch experiments with BOF slag aggregates and increasing initial phosphate concentrations showed phosphate removal efficiencies between 84 and 99% and phosphate removal capacities from 0.12 to 8.78 mg P/g slag. A continuous flow column experiment filled with BOF slag aggregates receiving an influent synthetic solution of 15 mg P/L during 213 days showed a removal efficiency greater than 99% and a phosphate removal capacity of 3.1 mg P/g slag. In both experiments the main P removal mechanism was found to be calcium phosphate precipitation which depends on Ca2þ and OH release from the BOF steel slag after dissolution of Ca(OH)2 in water. P saturation of slag was reached within the upper sections of the column which showed phosphate removal capacities between 1.7 and 2.5 mg P/g slag. Once Ca(OH)2 was completely dissolved in these column sections, removal efficiencies declined gradually from 99% until reaching stable outlet concentrations with P removal efficiencies around 7% which depended on influent Ca2þ for limited continuous calcium phosphate precipitation.
Published: 2015

18. Cryobanking in aquatic species: Applications and perspectives in fish germ cells

Author: Juan F. Asturiano, S. Martínez-Páramo, Ákos Horváth, Terrence R. Tiersch, Elsa Cabrita, Paz Herraez, Vanesa Robles, Catherine Labbé, Center of Marine Sciences, Partenaires INRAE, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Department of Aquaculture, Szent István University, Department of Molecular Biology, University of Leon, Instituto Español de Oceanografía, Instituto de Ciencia y Tecnologia Animal, Louisiana State University Agricultural Center, and ProdInra, Migration
Subjects: 0106 biological sciences, 010604 marine biology & hydrobiology, [SDV]Life Sciences [q-bio], Zoology, 04 agricultural and veterinary sciences, General Medicine, Anatomy, Biology, [INFO] Computer Science [cs], 01 natural sciences, General Biochemistry, Genetics and Molecular Biology, Cryopreservation, [SDV] Life Sciences [q-bio], Aquatic species, 040102 fisheries, 0401 agriculture, forestry, and fisheries, %22">Fish , Germ, [INFO]Computer Science [cs], 14. Life underwater, General Agricultural and Biological Sciences, ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2015

19. The adaptive response of bacterial food-borne pathogens in the environment, host and food: Implications for food safety

Author: Pierre Colin, Avelino Alvarez-Ordóñez, Christophe Nguyen-The, Véronique Broussolle, Miguel Prieto, Teagasc Agriculture and Food Development Authority (Teagasc), Sécurité et Qualité des Produits d'Origine Végétale (SQPOV), Avignon Université (AU)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Technopole Brest Iroise, Department of Food Hygiene and Technology, University of Leon, and Prieto, Miguel
Subjects: Food Safety, [SDV]Life Sciences [q-bio], Context (language use), Environment, Biology, medicine.disease_cause, Stress, Microbiology, Foodborne Diseases, Bacillus cereus, Stress, Physiological, Salmonella, réponse au stress, [SDV.IDA]Life Sciences [q-bio]/Food engineering, medicine, Acid, Animals, Humans, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, fluidité membranaire, adaptation au froid, 2. Zero hunger, Bacteria, business.industry, Host (biology), Pathogenic bacteria, General Medicine, Adaptive response, Food safety, biology.organism_classification, Adaptation, Physiological, Biotechnology, Gastrointestinal Tract, réponse adaptative, sécurité alimentaire, Food borne, SPANC, Host-Pathogen Interactions, Adaptive reponse, Mutation, Food Microbiology, Cold, salmonella typhimurium, business, rpoS, tolérance au stress, Food Science
Abstract: International audience; Bacteria are constantly faced to stress situations in their ecological niches, the food and the host gastrointestinal tract. The capacity to detect and respond to surrounding changes is crucial for bacterial pathogens to survive or grow in changing environments. To this purpose, cells have evolved various sophisticated networks designed to protect against stressors or repair damage caused by them. Challenges can occur during production of foods when subjected to processing, and after food ingestion when confronted with host defensive barriers. Some pathogenic bacteria have shown the capacity to develop stable resistance against extreme conditions within a defined genomic context and a limited number of generations. On the other hand, bacteria can also respond to adverse conditions in a transient manner, through the so-called stress tolerance responses. Bacterial stress tolerance responses include both structural and physiological modifications in the cell and are mediated by complex genetic regulatory machinery. Major aspects in the adaptive response are the sensing mechanisms, the characterization of cell defensive systems, such as the operation of regulatory proteins (e.g. RpoS), the induction of homeostatic and repair systems, the synthesis of shock response proteins, and the modifications of cell membranes, particularly in their fatty acid composition and physical properties. This article reviews certain strategies used by foodborne bacteria to respond to particular stresses (acid, cold stress, extreme pressure) in a permanent or transient manner and discusses the implications that such adaptive responses pose for food safety.
Published: 2015

20. Motility of liquid stored ram spermatozoa is altered by dilution rate independent of seminal plasma concentration

Author: S.P. de Graaf, Guillaume Tsikis, Xavier Druart, M. Mata-Campuzano, Felipe Martínez-Pastor, Clément Soleilhavoup, INDEGSAL, University of Leon, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Faculty of Veterinary Science, University of Sydney, FPI (Spanish Ministry of Science and Innovation), French Region Centre doctoral fellowship, Spanish R&D National Plan (AGL2010-15758), Australian Wool Innovation and the NSW Stud Merino Breeders Association Trust, Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)
Subjects: Male, endocrine system, [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], preservation, Motility, Semen, Biology, Andrology, Endocrinology, Food Animals, Animals, reproductive and urinary physiology, Sheep, urogenital system, General Medicine, Rate independent, Spermatozoa, 3. Good health, Dilution, ai, Plasma concentration, Sperm Motility, Animal Science and Zoology, Progressive spermatozoa, ram semen, Spermatozoa motility, Semen Preservation
Abstract: The fertility after use of liquid stored ram semen following cervical AI rapidly decreases if semen is stored beyond 12 h. The dilution of seminal plasma is often cited as a key contributor to the diminished motility and fertility of ram spermatozoa subjected to liquid preservation. Two experiments were conducted to assess the effect of spermatozoa concentration (i.e. dilution rate) and percentage of seminal plasma on the motility and viability of liquid stored ram spermatozoa. In Experiment 1, semen was diluted to one of seven concentrations ranging from 0.2 to 1.4 × 109 spermatozoa/ml with milk and assessed for motility after 3 or 24 h of storage at 15 °C. In Experiment 2, semen was collected and washed to remove seminal plasma before re-dilution to 0.2–1.4 × 109 spermatozoa/ml with milk containing 0%, 20% or 40% (final v/v ratio) seminal plasma and assessed for viability and motility after 3 or 24 h of storage at 15 °C. Whereas motility was not affected by spermatozoa concentration after 3 h of storage, the proportion of progressive spermatozoa decreased after 24 h of storage when spermatozoa concentration was greater than 1.0 × 109 spermatozoa/ml. The duration of preservation and the spermatozoa concentration affected spermatozoa motility but had no impact on spermatozoa viability. This negative effect of greater spermatozoa concentrations on motility was independent of the presence and the concentration of seminal plasma. The seminal plasma at both concentrations (20% and 40%) had a protective effect on spermatozoa motility after 24 h of storage. These findings have the potential to improve the efficiency of cervical AI with liquid stored ram semen.
Published: 2015

21. Dna methylation of fish germ cells and the risk of alteration after cryopreservation

Author: Labbé, Catherine, Depince, Alexandra, Milon, Pierre, Morini, M., Riesco, M., Robles, V., Asturiano, J., Horvath, A., Herraez, P., Laboratoire de Physiologie et Génomique des Poissons (LPGP), Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Recherche Agronomique (INRA), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institute of Animal Science and Technology, Universitat Politècnica de València (UPV), Universidad de León [León], Department of Aquaculture, Szent István University, Department of Molecular Biology, University of Leon, CRB-Anim project 'Investissements d'Avenir' ANR-11-INBS-0003, Center of Marine Sciences (CCMAR). PRT., Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), and ProdInra, Migration
Subjects: [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], [SDV.OT] Life Sciences [q-bio]/Other [q-bio.OT], ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2014

22. How are f2f sessions and telecollaboration linked into a coherent blended learning course?

Author: Nissen, Elke, LInguistique et DIdactique des Langues Étrangères et Maternelles (LIDILEM), Université Stendhal - Grenoble 3, University of Leon, and Nissen, Elke
Subjects: Virtual exchange, [SHS.EDU]Humanities and Social Sciences/Education, [SHS.EDU] Humanities and Social Sciences/Education, Blended learning, Telecollaboration
Abstract: International audience; If blended learning (BL) is defined as a mix of « classroom education » (Bersin 2004 : 85) and online learning (eg. Charlier et al. 2006, Garrison & Vaughan 2008, Neumeier 2005), telecollaboration can be considered as a specific type of BL where an online communication project with geographically distant partners is combined with f2f sessions. The manifold possibilities BL offers to enhance pedagogy in Higher Education, since it enables to take advantage of each of both learning modes, have been highlighted by many authors (eg. Deschryver & Charlier 2012, Garrison & Vaughan 2008, MacDonald 2008) and have made it to a widespread form of learning and teaching today. But, at the same time, the mix of learning modes that makes its richness is also what makes it difficult to design and to moderate. In despite (or maybe because) of this fact, the books providing guidelines and illustrating best practice most often simply show what kinds of activities should be done in each learning mode, but do not give hints on how to link the learning modes in a coherent way (Bersin 2004, MacDonald 2008). Or, if ever they do so (such as Garrison & Vaughan 2008: 105-141), they implicitly consider the f2f sessions as the dominant learning mode, completed by the online mode . But, in telecollaboration, where the design and preparation of a common project with distant partners is time-consuming (Guth et al. 2012), the online mode therefore presumably often becomes the central and dominant mode. Regarding telecollaboration, several publications focus on the challenges of setting up and running such a project (Ritter & O’Dowd 2006, O’Dowd 2007, Guth et al. 2012). But, here again, to our knowledge no specific emphasis is put on the parameters that determine efficacious integration of online interaction with distant partners and f2f sessions. Therefore, the question this presentation will tackle is: which parameters are used for a successful combination of telecollaboration and f2f sessions into a coherent course? Answering this question will help teachers to better integrate both learning modes. We will conduct an exploratory study in which we will interview five experienced teachers about their practice, in order to identify common approaches. The study will exclusively focus on language and/or intercultural training in Higher Education. It will be based on the parameters of existing BL typologies (Bersin 2004; Deschryver & Charlier 2012), but adapt them in order to meet the specific needs of this study. First, we will identify the dominant learning mode of these BL courses and see whether this choice is linked to the learning objectives (Nissen 2006), to the duration of the telecollaboration project, to institutional recognition (Guth et al. 2012) or to other aspects. Hence, does the telecollaboration project become central for course design or is it more an add-on activity? In addition, we want to find out which is the “thread” that determines the coherence of the course (is it task completion? Focus on form? A specific topic? etc.) Second, the study aims at analysing how the learning modes complete each other. Thus, which competences are trained in which mode (e.g. students benefit from proactive training of online discourse’s specificities, Thorne & Fischer 2012: §50; training that can take place f2f), when, why then, which role does the teacher play in each mode, and how does assessment take into account the contribution of two different learning modes to the course? Finally, based on the results of our study, we will highlight the basic parameters that make these language courses coherent in the eyes of their designer and tutor.
Published: 2014

23. Cryopreservation of gametes for aquaculture and alternative cell sources for genome preservation

Author: Catherine Labbé, Vanesa Robles, María Paz Herráez, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), University of Leon, Absent, Allan Geoff, and Burnell Gavin
Subjects: nuclear transfer, [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], animal structures, Cryoprotectant, crystallization, Somatic cell, Cell, Sperm cryopreservation, Zoology, Biology, Genome, Cryopreservation, 03 medical and health sciences, 0302 clinical medicine, Aquaculture, Botany, medicine, 14. Life underwater, cell transplantation, 030304 developmental biology, chromatin structure, 0303 health sciences, 030219 obstetrics & reproductive medicine, business.industry, cryoprotectant, Embryonic stem cell, medicine.anatomical_structure, business
Abstract: In this chapter the benefits of short- to long-term storage of genetic material from aquatic species in liquid nitrogen are introduced. The biophysical and chemical challenges that arise during freeze-thawing are presented from the perspective of the large degree of structural and biological diversity found in fish, mollusc and crustacean gametes. Sperm cryopreservation procedures and the challenges raised by cryopreservation of oocytes and embryos or larvae are then discussed with a special focus on the importance of the genetic integrity of the thawed cells. Alternatives to the cryobanking of gametes and embryos rely on the cryopreservation of cells of somatic, embryonic and germinal origin. The challenges posed by the use of such cells to reconstruct fish are then described.
Published: 2013

24. Gamete quality and broodstock management in temperate fish

Author: María Paz Herráez, Elsa Cabrita, Brendan McAndrew, Herve Migaud, J. Gordon Bell, Manuel Carrillo, Andrew Davie, Julien Bobe, Institute of Aquaculture, University of Stirling, Institute of Marine Sciences of Andalusia, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Recherche Agronomique (INRA), Department of Molecular Biology, University of Leon, Institute of Aquaculture of Torre de la Sal, LARVANET - COST ACTION FA 0801 (EU RTD framework Program) & EC project PROSPAWN, and Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )
Subjects: Fish farming, media_common.quotation_subject, Captivity, Broodstock, Management, Monitoring, Policy and Law, Aquatic Science, Biology, 03 medical and health sciences, Aquaculture, Quality (business), 14. Life underwater, Domestication, 030304 developmental biology, media_common, Sustainable development, 0303 health sciences, Ecology, business.industry, [SDV.BA]Life Sciences [q-bio]/Animal biology, [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology, 04 agricultural and veterinary sciences, Fishery, Agriculture, 040102 fisheries, 0401 agriculture, forestry, and fisheries, business
Abstract: The ability to fully control sexual maturation and spawning and to produce large numbers of high quality seeds 'on demand' (i.e. all year long) is a primary requirement for the successful development of aquaculture. This relies on optimal broodstock management practices based on extensive knowledge of the nutritional and environmental requirements of fish in captivity. However, for many established, emerging and new farmed fish species, such knowledge is limited or not available yet. The level of domestication also plays an essential role as stocks with improved traits in farming conditions are selected. Importantly, reliable indicators of egg quality are still lacking and hatcheries still rely on wild harvested broodstocks in many farmed fish species. These key challenges must be addressed urgently to ensure the sustainable development of the European fish farming sector. This review gives an overview of methods to assess egg/sperm quality and many of the most important factors affecting gamete production and quality, including broodstock nutrition, environmental and spawning induction protocols, genetic management, gamete preservation and new reproductive strategies. © 2013 Wiley Publishing Asia Pty Ltd., This study benefited from participation in LARVANET COST action FA0801 (EU RTD framework Program) and EC project PROSPAWN.
Published: 2013

25. Refrigeration of ram semen in presence of seminal plasma affects sperm chromatin structure without shortening telomeres

Author: Mata-Campuzano, M., Ordas, L., Carton-Garia, F., Paz, P., Vanesa Robles, Druart, X., Martinez-Pastor, F., INDEGSAL, University of Leon, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), European Society for Domestic Animals. Dublin, IRL., and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)
Subjects: [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2012

26. Identification versus counting protocols as sources of uncertainty in diatom-based ecological status assessments

Author: Saúl Blanco, Adrienne Mertens, Bryan Kennedy, Michel Coste, Petr Marvan, Markéta Fránková, P. Ivanov, Juha Miettinen, Maria Kahlert, Raino-Lars Albert, Andrea Vogel, Joanna Picinska-Fałtynowicz, Sirje Vilbaste, Juliette Rosebery, Tomáš Bešta, Elisabet Tornés, Martyn Kelly, Daša Hlúbiková, Luc Denys, Salomé F.P. Almeida, Luc Ector, Swedish University of Agricultural Sciences (SLU), BOWBURN CONSULTANCY DURHAM GBR, Partenaires IRSTEA, Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA), ECOMONITOR LTD JOENSUU FIN, UNIVERSITY OF AVEIRO PRT, UNIVERSITY OF SOUTH BOHEMIA CESKE BUDEJOVICE CZE, UNIVERSITY OF LEON ESP, Réseaux épuration et qualité des eaux (UR REBX), Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA), Research Institute for Nature and Forest (INBO), DEPARTMENT OF ENVIRONMENT AND AGRO BIOTECHNOLOGIES BELVAUX LUX, INSTITUTE OF BOTANY BRNO CZE, FACULTY OF BIOLOGY SOFIA BGR, EPA MAYO IRL, LIMNI SRO BRNO CZE, GRONTMIJ NEDERLAND BV AMSTERDAM NLD, IMGW INSTITUTE OF METEOROLOGY AND WATER MANAGEMENT WROCLAW POL, UNIVERSITY OF GIRONA ESP, ESTONIAN UNIVERSITY OF LIFE SCIENCES TARTU EST, and SEEFELD HECHENDORF DEU
Subjects: 0106 biological sciences, Protocol (science), European level, biology, Ecology, 010604 marine biology & hydrobiology, Sample (statistics), Aquatic Science, biology.organism_classification, 010603 evolutionary biology, 01 natural sciences, Identification (information), Diatom, Geography, Water Framework Directive, [SDE]Environmental Sciences, European standard, Metric (unit)
Abstract: International audience; In 2009, seventeen analysts participated in a pan-European diatom ring-test (intercalibration), analyzing nine samples from seven countries following the European standard EN 14407. The objective of this exercise was to agree on practical conventions on diatom identification to facilitate future intercalibration work and to assess the extent to which national differences in sample analysis (counting protocol and identification conventions) contribute to variability in EU-level comparisons of diatom-based methods. Differences in the reported taxa lists were large, but not a major source of variation in values of a common metric (the phytobenthos Intercalibration Common Metric, ICM). Therefore, every country can apply its own identification conventions for national assessments, and still be fairly confident that the ICM reflects the national classification of its streams. Part of the index variation was due to differences in counting protocols and care should be taken when handling broken valves, girdle views and small taxa. More work at both national and European level is needed to provide a harmonized way of using diatoms for ecological status assessments in the future.
Published: 2012

27. Patrons de réponse des diatomées en rivières contaminées par les métaux

Author: Soizic Morin, Helena Guasch, Isabelle Lavoie, Martin Laviale, Berta Bonet, Michel Coste, Elisabet Tornés, Eloy Bécares, Natàlia Corcoll, Thi Thuy Duong, Claude Fortin, Leslie Faggiano, Arielle Cordonier, Florence Peres, Saúl Blanco, Adeline Arini, Agnès Feurtet-Mazel, Sergi Sabater, Réseaux épuration et qualité des eaux (UR REBX), Centre national du machinisme agricole, du génie rural, des eaux et forêts (CEMAGREF), SECOE GENEVE CHE, Partenaires IRSTEA, Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA), INRS EAU QUEBEC CAN, UMR 5805 Environnements et Paléoenvironnements Océaniques et Continentaux (EPOC), Observatoire aquitain des sciences de l'univers (OASU), Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), UNIVERSITY OF LEON ESP, INSTITUTE OF ENVIRONMENTAL TECHNOLOGY HANOI VNM, Instituto Catalán de Investigación del Agua - ICRA (SPAIN) (ICRA), UNIVERSITY OF GIRONA ESP, Génétique et évolution des populations végétales (GEPV), Université de Lille, Sciences et Technologies-Centre National de la Recherche Scientifique (CNRS), Asconit Consultants, Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA), UNIVERSIDADE DE AVEIRO PRT, H. Guasch, A. Ginebreda, and A. Geiszinger
Subjects: 0106 biological sciences, BIOVOLUME, biology, Ecology, Nitzschia, 010604 marine biology & hydrobiology, Species distribution, Community structure, Pinnularia, 010501 environmental sciences, Contamination, biology.organism_classification, 01 natural sciences, 6. Clean water, Fragilaria, TRAIT, Diatom, 13. Climate action, Environmental chemistry, [SDE]Environmental Sciences, Environmental science, 14. Life underwater, Water quality, 0105 earth and related environmental sciences
Abstract: International audience; Diatoms play a key role in the functioning of streams, and their sensitivity to many environmental factors has led to the development of numerous diatom-based indices used in water quality assessment. Although diatom-based monitoring of metal contamination is not currently included in water quality monitoring programs, the effects of metals on diatom communities have been studied in many polluted watersheds as well as in laboratory experiments, underlying their high potential for metal contamination assessment. Here, we built large database of river diatoms (comprising more than 600 taxa) that were exposed to various loads of heavy metals in the water was investigated. The samples were collected during field surveys carried out in 6 different countries (France, Spain, Switzerland, Canada, Vietnam, China). After taxonomy harmonization, the patterns in diatom community structure were investigated for 202 samples, all collected from hard substrates in rivers with circumneutral water pH. As the sites were contaminated by a mixture of different metals (mainly Al, As, Cd, Cr, Cu, Fe, Hg, Ni, Pb, Zn) with various loads, metal concentrations were converted into a single score after Clements et al. (2000) in order to classify sites according to potential metal toxicity. Metal contamination proved to be a strong driver of the community structure, and enabled for the identification of tolerant species like Eolimna minima, Surirella angusta, Cocconeis placentula var. euglypta or Pinnularia parvulissima. Traits were also tested: diatom cell size and the occurrence of diatom deformities were found to be good indicators of high metal contamination. This work provides a basis for further use of diatoms as indicators of metal pollution Clements WH, Carlisle DM, Lazorchak JM, Johnson PC, 2000. Heavy metals structure benthic communities in Colorado mountain streams.
Published: 2011

28. Stable patterns in diatom species distributions in metal-contaminated environments

Author: Morin, Soizic, Cordonier, Arielle, Lavoie, Isabelle, Arini, Adeline, Blanco, Saul, Duong, T.T., Tornes, Elisabet, Bonet, Berta, Corcoll, Natàlia, Faggiano, Leslie, Laviale, M., Pérès, F., Becares, E., Coste, Michel, Feurtet Mazel, A., Fortin, Claude, Guasch, H., Sabater, Sergi, Réseaux épuration et qualité des eaux (UR REBX), Centre national du machinisme agricole, du génie rural, des eaux et forêts (CEMAGREF), SECOE GENEVE CHE, Partenaires IRSTEA, Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA), INRS QUEBEC CAN, Université de Bordeaux (UB), UNIVERSITY OF LEON ESP, INSTITUTE OF ENVIRONMENTAL TECHNOLOGY HANOI VNM, ICRA GIRONA ESP, INSTITUTE OF AQUATIC ECOLOGY GIRONA ESP, Universitat de Girona [Girona], Universitat de Girona (UdG), Mer, molécules et santé EA 2160 (MMS), Le Mans Université (UM)-Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-Université de Nantes - UFR des Sciences Pharmaceutiques et Biologiques, Université de Nantes (UN)-Université de Nantes (UN), Universidad de León [León], Institut National de la Recherche Scientifique [Québec] (INRS), and Irstea Publications, Migration
Subjects: [SDE] Environmental Sciences, [SDE]Environmental Sciences
Abstract: International audience; Une large base de données de diatomées d'eau douce (comportant plus de 600 taxons après harmonisation taxonomique) ayant exposées à divers niveaux de contamination en métaux dissous a été constituées. Elle rassemble environ 200 échantillons périphytiques provenant de cours d'eau circumneutres de 6 pays différents (France, Espagne, Suisse, Canada, Vietnam, Chine). Les sites étant contaminés par différents métaux en mélange (principalement Al, as, Cd, Cr, Cu, Fe, Hg, Ni, Pb, Zn) en concentrations variables, ces concentrations ont été converties en un score simplifié d'après Clements et al. (2000), afin de déterminer 4 catégories d'exposition : niveau de base, faible, modéré et élevé. La biotypologie (c.-à-d., la classification basée sur la structure spécifique des communautés de diatomées) indique que les espèces sont influencées par la biogéographie, cependant les apports métalliques constituent un paramètre structurant fort, et l'analyse a permis de déterminer les sensibilités / tolérances d'espèces particulières (par exemple Eolimna minima, Nitzschia palea, Surirella angusta). La pertinence des formes tératologiques pour le biomonitoring des pollutions de métaux lourds est également soulignée, dans le cas de niveaux de contamination élevés.
Published: 2010

29. Revealing the History of Sheep Domestication Using Retrovirus Integrations

Author: Ingrid Mainland, Metehan Uzun, Mohammad Hossein Banabazi, Rudovick Kazwala, Josephine M. Pemberton, Frederick Arnaud, Félix Goyache, Massimo Palmarini, N. S. Marzanov, Paolo Ajmone-Marsan, Rowland R. Kao, Alberto Alberti, Bernardo Chessa, Bahy A. Ali, Urmas Saarma, Filipe Pereira, Ya-Ping Zhang, Michel M. Dione, Sohail Ahmad, Emma Eythorsdottir, Dario Beraldi, Michael William Bruford, Leopoldo Iannuzzi, Juan J. Arranz, Zhiliang Wang, Marco Pittau, Ingrid Olsaker, Lars-Erik Holm, Juha Kantanen, Michael J. Stear, Martin J. Holland, António Amorim, Thomas E. Spencer, Faculty of Veterinary Medicine, Institute Comparative Medicine, University of Glasgow, Dipartimento Patologio & Clinica Veterinaria, University of Sassari, Institute Patology and Imunology Molecular, Fac Ciencias, Universidade do Porto, Faculty Veterinary Medicine, Institute Comparative Medicine, Area Genetic and Reproduction Animale, SERIDA, Division Archael Geography and Environmental Science, University of Bradford, School Biology Science, Institute Evolutionary Biology, University of Edinburgh, Herzberg Institute of Astrophysics, Conseil National de Recherches Canada (CNRC), Animal Science Research Institute Iran, Department of Biotechnology, Institute of Iran, Department Veterinary Medicine and Public Health, Sokoine University of Agriculture (SUA), Kunming Institute of Zoology, tate Key Laboratory of Genetic Resources and Evolution, Chinese Academy of Sciences [Changchun Branch] (CAS), Faculty Veterinary, Department Animal Production, University of Leon, Engineering and Biotechnology Research Institute, Mubarak City, National Diagnostic Center for Exotic Animal Disease, China Animal Health and Epidemiology Center, School Health Science, Çanakkale Onsekiz Mart University (COMU), Intitute Trypanotolerance Center, Department Basic Science and Aquatic Medicine, Norwegian School of Veterinary Science, Faculty of Agricultural Science, Department Genetic and Biotechnology, Aarhus University [Aarhus], Institute Ecologic and Earth Science, Department of Zoology, University of Tartu, Agricultural University Peshawar, All Russian Research Institute Animal Husbandry, Russian Academy of Agricultural Sciences (RAAS), Agricultural University of Iceland, MRC Labs, School Hygiene and Tropical Medicine, City University London, Istitut Zootecnie, Università cattolica del Sacro Cuore [Milano] (Unicatt), School of Biosciences [Cardiff], Cardiff University, Biotechnology and Food Research, Agrifood Research Finland, Center Animal Biotechnology and Genomic, Texas A&M University System, Biotechnology and Biological Sciences Research Council BB/F014643/1, NICHD NIH HHS HD05274 R01 HD052745, and Wellcome Trust 076522 081696
Subjects: sheep, mouton, [SDV]Life Sciences [q-bio], Endogenous retrovirus, Zoology, Population genetics, Bovidae, NEAR-EAST, MITOCHONDRIAL, LINEAGES, ORIGIN, WILD, 03 medical and health sciences, domestication, Retrovirus, Domestication, General, 030304 developmental biology, wether hoggs, 2. Zero hunger, 0303 health sciences, Multidisciplinary, biology, Settore AGR/17 - ZOOTECNICA GENERALE E MIGLIORAMENTO GENETICO, business.industry, 0402 animal and dairy science, 04 agricultural and veterinary sciences, biology.organism_classification, 040201 dairy & animal science, domestication animale, Mouflon, retrovirus, Genetic distance, rétrovirus endogène, Livestock, business
Abstract: The domestication of livestock represented a crucial step in human history. By using endogenous retroviruses as genetic markers, we found that sheep differentiated on the basis of their "retrotype" and morphological traits dispersed across Eurasia and Africa via separate migratory episodes. Relicts of the first migrations include the Mouflon, as well as breeds previously recognized as "primitive" on the basis of their morphology, such as the Orkney, Soay, and the Nordic short-tailed sheep now confined to the periphery of northwest Europe. A later migratory episode, involving sheep with improved production traits, shaped the great majority of present-day breeds. The ability to differentiate genetically primitive sheep from more modern breeds provides valuable insights into the history of sheep domestication.
Published: 2009

30. Bibliometrics : representation instrument of the multidisciplinary positioning of a scientific area. Implementation for an Advisory Scientific Committee

Author: Rostaing, Hervé, Barts, Nicolas, Léveillé, Valérie, Laboratoire des Sciences de l'Information et des Systèmes (LSIS), Centre National de la Recherche Scientifique (CNRS)-Arts et Métiers Paristech ENSAM Aix-en-Provence-Université de Toulon (UTLN)-Aix Marseille Université (AMU), Centre de recherche rétrospective de Marseille (CRRM), Université Paul Cézanne - Aix-Marseille 3, ISKO-Spain, University of Leon, 18-20 avril, Rostaing, Hervé, and Aix Marseille Université (AMU)-Université de Toulon (UTLN)-Arts et Métiers Paristech ENSAM Aix-en-Provence-Centre National de la Recherche Scientifique (CNRS)
Subjects: Scientific portfolio analysis, Strategic analysis of research, Bibliometric study, Advisory scientific committee, [SHS.INFO]Humanities and Social Sciences/Library and information sciences, Self-evaluation, [SHS.INFO] Humanities and Social Sciences/Library and information sciences, Competitive positioning, Multidisciplinary bibliometric analysis
Abstract: International audience; Bibliometric techniques study researchers' communication activity to have a better understanding of phenomena of construction, dissemination and use of scientific and technical knowledge. In this paper, a bibliometric study was used like an assistance instrument for the strategic positioning of a scientific area. The aim of this bibliometric study was to help the work of an Advisory Scientific Committee by offering a global and "objective" view of the position and the evolution of the scientific activity of the Aix-Marseilles area. The methodology employed was strongly inspired by strategic analysis methods carried out in companies. The implemented strategic analysis process involves the definition of strategic scientific units, the selection of competitors (a set of scientific areas), the choice of measuring criterion of the scientific activity, the evaluation of the dynamics over the years and the graphical representation of the scientific portfolio of the Aix-Marseille area. Such a strategic analysis process for analyzing a scientific portfolio allows positioning the strategic scientific fields of the Aix-Marseilles area in relation to the "competition" by taking into account the dynamic history of its activity.
Published: 2007

31. Kinematics of the North American–Caribbean-Cocos plates in Central America from new GPS measurements across the Polochic-Motagua fault system

Author: O. Porras, Eric Barrier, L. Chiquin, Cécile Lasserre, E. Molina, I. Arzu, A. Franco, Omar Flores, T. Duquesnoy, J. Requena, Hélène Lyon-Caen, R. Wolf, O. Galicia, M. Chiquin, J. Luna, V. Robles, J. Romero, Laboratoire de géologie de l'ENS (LGE), Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Département des Géosciences - ENS Paris, École normale supérieure - Paris (ENS Paris)-École normale supérieure - Paris (ENS Paris), Laboratoire de tectonique (LT), Centre National de la Recherche Scientifique (CNRS)-Université de Cergy Pontoise (UCP), Université Paris-Seine-Université Paris-Seine-Institut national des sciences de l'Univers (INSU - CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC), Laboratoire de Géophysique Interne et Tectonophysique (LGIT), Observatoire des Sciences de l'Univers de Grenoble (OSUG), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Laboratoire Central des Ponts et Chaussées (LCPC)-Institut des Sciences de la Terre (ISTerre), Université Grenoble Alpes (UGA)-Centre National de la Recherche Scientifique (CNRS)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-PRES Université de Grenoble-Institut de recherche pour le développement [IRD] : UR219-Institut national des sciences de l'Univers (INSU - CNRS)-Institut Français des Sciences et Technologies des Transports, de l'Aménagement et des Réseaux (IFSTTAR)-Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-PRES Université de Grenoble-Institut de recherche pour le développement [IRD] : UR219-Institut Français des Sciences et Technologies des Transports, de l'Aménagement et des Réseaux (IFSTTAR), Centro Universitario del Norte, Universidad de San Carlos de Guatemala, Centro de Estudios Superiores de Energia y Minas, Instituto Nacional de Sismologia, Vulcanologia, Meteorologia y Hidrologia (INSIVUMEH) (INSIVUMEH), INSIVUMEH, Department of Molecular Biology, Faculty of Biology, Campus Vegazana s/n, University of Leon, Geologia Ambiental e Economia, Laboratory of Atmospheric Chemistry [Paul Scherrer Institute] (LAC), Paul Scherrer Institute (PSI), École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL), Institut des Sciences de la Terre [2011-2015] (ISTerre [2011-2015]), Université Joseph Fourier - Grenoble 1 (UJF)-Institut Français des Sciences et Technologies des Transports, de l'Aménagement et des Réseaux (IFSTTAR)-Institut national des sciences de l'Univers (INSU - CNRS)-Institut de recherche pour le développement [IRD] : UR219-PRES Université de Grenoble-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut Français des Sciences et Technologies des Transports, de l'Aménagement et des Réseaux (IFSTTAR)-Institut national des sciences de l'Univers (INSU - CNRS)-Institut de recherche pour le développement [IRD] : UR219-PRES Université de Grenoble-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS)-Laboratoire Central des Ponts et Chaussées (LCPC)-Observatoire des Sciences de l'Univers de Grenoble [1985-2015] (OSUG [1985-2015]), Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology [2007-2019] (Grenoble INP [2007-2019])-Institut national des sciences de l'Univers (INSU - CNRS)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology [2007-2019] (Grenoble INP [2007-2019])-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de géologie de l'ENS (LGENS), Institut des Sciences de la Terre de Paris (iSTeP), Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Institut national des sciences de l'Univers (INSU - CNRS), Laboratoire Central des Ponts et Chaussées (LCPC)-Observatoire des Sciences de l'Univers de Grenoble (OSUG), and Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut national des sciences de l'Univers (INSU - CNRS)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut national des sciences de l'Univers (INSU - CNRS)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)
Subjects: [SDU.STU.TE]Sciences of the Universe [physics]/Earth Sciences/Tectonics, geography, geography.geographical_feature_category, 010504 meteorology & atmospheric sciences, Volcanic arc, business.industry, [SDU.STU.GP]Sciences of the Universe [physics]/Earth Sciences/Geophysics [physics.geo-ph], Triple junction, Kinematics, Slip (materials science), 010502 geochemistry & geophysics, Geodesy, 01 natural sciences, Plate tectonics, Geophysics, Sinistral and dextral, Global Positioning System, General Earth and Planetary Sciences, business, Geology, Seismology, ComputingMilieux_MISCELLANEOUS, 0105 earth and related environmental sciences
Abstract: [1] The Polochic-Motagua strike-slip fault system in Guatemala marks the on-land plate boundary between the North American (NA) and the Caribbean (CA) plates. GPS observations in 1999 and 2003 show that the far-field velocity across the system (NA-CA relative velocity) is ∼20 mm/yr. This is significantly higher than the NUVEL-1A velocity but is consistent with the GPS based CA-NA velocity proposed by DeMets et al. (2000). The observations are modeled by a fault centered on the Motagua fault, locked at a depth of 20 km, with a slip-rate decreasing from eastern to central Guatemala from 20 to 12 mm/yr towards the NA-CA-Cocos triple junction. This decrease is accommodated by ∼8 mm/yr of E-W extension in the westernmost part of CA south of the Motagua fault. About 10 mm/yr of dextral slip is observed across the Mid-American Volcanic Arc. The NA-CA-Cocos triple junction is thus a complex, ∼400 km-wide wedge-shaped area.
Published: 2006

32. Relationship of Weather Types on the Seasonal and Spatial Variability of Rainfall, Runoff, and Sediment Yield in the Western Mediterranean Basin

Author: Álvaro Gómez-Gutiérrez, Feliciana Licciardello, Marcella Biddoccu, Susana Bernal, Victor Castillo, José Carlos González-Hidalgo, Costas Kosmas, Ramón Bienes, Juan Albaladejo, Susanne Schnabel, Artemio Cerdà, Ana Lucía, José Damián Ruiz-Sinoga, Luis Merino-Martín, Xavier Úbeda, Yolanda Cantón, Emmanouil A. Varouchakis, Francesc Gallart, Antonio Canatário-Duarte, Vincenzo Pampalone, Patricio Cid, Dhais Peña-Angulo, N. Mathys, José Andrés López-Tarazón, Jérôme Latron, Sébastien Klotz, Matija Zorn, Damien Raclot, Elvira Díaz-Pereira, Maria Jose Marques, María Martínez-Mena, Óscar González-Pelayo, Albert Solé-Benet, E. Gimeno, María Fernández-Raga, Noemí Lana-Renault, Carla Ferreira, George P. Karatzas, N. Moustakas, Helena Gómez-Macpherson, Francesca Todisco, Teodoro Lasanta, C. Le Bouteiller, Joan Estrany, Vicente Andreu, M. L. Rodríguez-Blanco, José M. Nicolau, José Luis Rubio, Orestis Kairis, M. Mercedes Taboada-Castro, Miguel A. Campo-Bescós, Eugenio Cavallo, Tíscar Espigares, Saskia Keesstra, Luciano Mateos, H. Bahri, José A. Gómez, Y. Le Bissonnais, Jesús Rodrigo-Comino, José María Senciales-González, A. Zabaleta, Lea Wittenberg, Mariano Moreno-de las Heras, Vito Ferro, M.T. Taboada-Castro, Javier Casalí, Nicola Cortesi, Rafael Giménez, Asunción Romero-Díaz, C. Marín, Estela Nadal-Romero, J. Farguell, Julián Campo, José Martínez-Fernández, G. Desir, V.M. Marín-Moreno, Roberto Lázaro, Encarnación V. Taguas, Instituto Universitario de Ciencias Ambientales (IUCA), Departamento de Geografía, University of Zaragoza - Universidad de Zaragoza [Zaragoza], Departamento de Química Física, Facultad de Ciencias Quimicas, Desertification Research Centre, Department of Environmental Quality and Soils, Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Institut National de Recherche en Génie Rural Eaux et Forêts (INRGREF), Ecole Nationale du Génie Rural, des Eaux et des Forêts (ENGREF)-Institution de la Recherche et de l'Enseignement Supérieur Agricoles [Tunis] (IRESA), Integrative Freshwater Ecology Group, Centre d’Estudis Avançats de Blanes, Institute for Agricultural and Earthmoving Machines, National Research Council of Italy | Consiglio Nazionale delle Ricerche (CNR), Departamento Investigación Aplicada y Extensión Agraria, Instituto Madrileño de Investigación y Desarrollo Rural, Agrario y Alimentario (IMIDRA), ISFOOD Institute, Department of Projects and Rural Engineering, Universidad Pública de Navarra [Espagne] = Public University of Navarra (UPNA), School of Agriculture, Food and Rural Development, Department of Agronomy (Soil Science Area), University of Almeria, Soil and Water Conservation Research Group, Centro de Edafologia y Biologia Aplicada del Segura, Department of Geography, Soil Erosion and Degradation Research Group, Universitat de València (UV), Laboratorio Nacional de Genómica para la Biodiversidad (LANGEBIO), Maize Genetics and Genomics Lab, CINVESTAV, Department of Earth Sciences, Barcelona Supercomputing Center - Centro Nacional de Supercomputacion (BSC - CNS), Departamento de Ciencias de la Tierra, Departamento de Ciencias de la Vida, Unidad de Ecología, Universidad de Alcalá de Henares (UAH), Department of Geography, Mediterranean Ecogeomorphological and Hydrological Connectivity Research Team (MEDhyCON), University of the Balearic Islands (UIB), Mediterranean Environmental Research Group, Department of Physical Geography and Regional Geographic Analysis (GRAM), University of Barcelona, Department of Applied Chemistry and Physics, University of Leon, College of Agriculture, Research Centre for Natural Resources, Environment and Society (CERNAS), Instituto Politécnico de Coimbra, Department of Earth and Marine Sciences [Palermo], Università degli studi di Palermo - University of Palermo, Institute of Earth Sciences Jaume Almera, Instituto de Agricultura Sostenible - Institute for Sustainable Agriculture (IAS CSIC), INTERRA Research Institute, Universidad de Extremadura - University of Extremadura (UEX), Department of Environment and Planning, Earth Surface Processes Team (ESP) Centre for Environmental and Marine Studies (CESAM), Universidade de Aveiro, Department of Natural Resources Management and Agricultural Engineering, Agricultural University of Athens, School of Environmental Engineering [Chania], Technical University of Crete [Chania], Team Soil Water and Land Use, Wageningen Environmental Research, Wageningen UR, Erosion torrentielle neige et avalanches (UR ETGR (ETNA)), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Area of Physical Geography, DCH, Universidad de la Rioja, Instituto Pirenaico de Ecologìa = Pyrenean Institute of Ecology [Zaragoza] (IPE - CSIC), Institute of Environmental Assessment and Water Research (IDAEA), Experimental Station of Arid Zones (EEZA), Consejo Superior de Investigaciones Científicas, Laboratoire d'étude des Interactions Sol - Agrosystème - Hydrosystème (UMR LISAH), Institut de Recherche pour le Développement (IRD)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro - Montpellier SupAgro, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Physical and Regional Geography Research Group, Université Catholique de Louvain = Catholic University of Louvain (UCL), Universitat de Lleida, University of Potsdam = Universität Potsdam, Faculty of Sciences, Center for Applied Geosciences, Eberhard Karls Universität Tübingen = Eberhard Karls University of Tuebingen, Universidad de Córdoba = University of Córdoba [Córdoba], Universidade do Porto = University of Porto, Instituto Hispano Luso de Investigaciones Agrarias, Universidad de Salamanca, Botanique et Modélisation de l'Architecture des Plantes et des Végétations (UMR AMAP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre d’Ecologie Fonctionnelle et Evolutive (CEFE), Université Paul-Valéry - Montpellier 3 (UPVM)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro - Montpellier SupAgro, Department of Agricultural, Food and Forest Sciences, Laboratoire commun de télédétection (UR LCMO), Centre national du machinisme agricole, du génie rural, des eaux et forêts (CEMAGREF), University of Vigo [ Pontevedra], Physical Geography, Trier University, Departamento de Geografía, Universidad de Cantabria [Santander], Universidad de Málaga [Málaga] = University of Málaga [Málaga], Faculty of Sciences, Centre for Advanced Scientific Research (CICA), University of A Coruña (UDC), Department of Agriculture-Food and Environmental Sciences, Agricultural and Biosystems Engineering Research Unit, Università degli Studi di Perugia = University of Perugia (UNIPG), Departamento de Geografía, Grup de Recerca Ambiental Mediterrània, Department of Geography and Environmental Studies, University of Nairobi (UoN), Science and Technology Faculty, Hydro-Environmental Processes Research Group, University of the Basque Country (University of the Basque Country), Geographical Institute, Scientific Research Center of the Slovenian Academy of Sciences and Arts (ZRC SAZU), Spanish Government (Ministry of Economy and Competitiveness, MINECO) and FEDER Projects: CGL2014 52135-C3-3-R, ESP2017-89463-C3-3-R, CGL2014-59946-R, CGL2015-65569-R, CGL2015-64284-C2-2-R, CGL2015-64284-C2-1-R, CGL2016-78075-P, GL2008-02879/BTE, LEDDRA 243857, RECARE-FP7, CGL2017-83866-C3-1-R, and PCIN-2017-061/AEI. Dhais Pena-Angulo received a 'Juan de la Cierva' postdoctoral contract (FJCI-2017-33652 Spanish Ministry of Economy and Competitiveness, MEC). Ana Lucia acknowledge the 'Brigitte-Schlieben-Lange-Programm'. The 'Geoenvironmental Processes and Global Change' (E02_17R) was financed by the Aragon Government and the European Social Fund. Jose Andres Lopez-Tarazon acknowledges the Secretariat for Universities and Research of the Department of the Economy and Knowledge of the Autonomous Government of Catalonia for supporting the Consolidated Research Group 2014 SGR 645 (RIUS-Fluvial Dynamics Research Group). Artemi Cerda thank the funding of the OCDE TAD/CRP JA00088807. Jose Martinez-Fernandez acknowledges the project Unidad de Excelencia CLU-2018-04 co-funded by FEDER and Castilla y Leon Government. Ane Zabaleta is supported by the Hydro-Environmental Processes consolidated research group (IT1029-16, Basque Government)., Ministerio de Economía y Competitividad (España), Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), European Commission, Gobierno de Aragón, Eusko Jaurlaritza, Generalitat de Catalunya, Junta de Castilla y León, Universidad Pública de Navarra. Departamento de Ingeniería, Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa. ISFOOD - Institute for Innovation and Sustainable Development in Food Chain, Nafarroako Unibertsitate Publikoa. Ingeniaritza Saila, University of Zaragoza, CNR - National Research Council of Italy, Universidad Pública de Navarra, Université de Valence, University of Extremadura, Instituto Pirenaico de Ecologia (IPE), Institut de Recherche pour le Développement (IRD)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), University of Potsdam, Eberhard Karls University Tübingen, Universidad de Córdoba [Cordoba], Instrumentation, Material and Correlated Electrons Physics (IMAPEC), PHotonique, ELectronique et Ingénierie QuantiqueS (PHELIQS), Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA), Universidade do Porto, Université Paul-Valéry - Montpellier 3 (UPVM)-Centre National de la Recherche Scientifique (CNRS)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM), Università degli Studi di Perugia (UNIPG), Barcelona Supercomputing Center, UAM. Departamento de Geología y Geoquímica, Pena-Angulo D., Nadal-Romero E., Gonzalez-Hidalgo J.C., Albaladejo J., Andreu V., Barhi H., Bernal S., Biddoccu M., Bienes R., Campo J., Campo-Bescos M.A., Canatario-Duarte A., Canton Y., Casali J., Castillo V., Cavallo E., Cerda A., Cid P., Cortesi N., Desir G., Diaz-Pereira E., Espigares T., Estrany J., Farguell J., Fernandez-Raga M., Ferreira C.S., Ferro V., Gallart F., Gimenez R., Gimeno E., Gomez J.A., Gomez-Gutierrez A., Gomez-Macpherson H., Gonzalez-Pelayo O., Kairis O., Karatzas G.P., Keesstra S., Klotz S., Kosmas C., Lana-Renault N., Lasanta T., Latron J., Lazaro R., Bissonnais Y.L., Bouteiller C.L., Licciardello F., Lopez-Tarazon J.A., Lucia A., Marin-Moreno V.M., Marin C., Marques M.J., Martinez-Fernandez J., Martinez-Mena M., Mateos L., Mathys N., Merino-Martin L., Moreno-de las Heras M., Moustakas N., Nicolau J.M., Pampalone V., Raclot D., Rodriguez-Blanco M.L., Rodrigo-Comino J., Romero-Diaz A., Ruiz-Sinoga J.D., Rubio J.L., Schnabel S., Senciales-Gonzalez J.M., Sole-Benet A., Taguas E.V., Taboada-Castro M.T., Taboada-Castro M.M., Todisco F., Ubeda X., Varouchakis E.A., Wittenberg L., Zabaleta A., Zorn M., Université Paul-Valéry - Montpellier 3 (UPVM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-École pratique des hautes études (EPHE), and Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)
Subjects: Mediterranean climate, Western Mediterranean, Rainfall, Atmospheric Science, Water en Landgebruik, 010504 meteorology & atmospheric sciences, Climate, analyses, Sedimentació, 02 engineering and technology, [SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics, Phylogenetics and taxonomy, 01 natural sciences, Mediterranean Basin, Soil, Bodem, Lluvia, Soil, Water and Land Use, Geología, weather types, rainfall, runoff, erosion, sediment yield, seasonal analyses, Mediterranean basin, Erosion, Mediterranean basin, Rainfall, Runoff, Seasonal analyses, Sediment yield, Weather types, 020701 environmental engineering, Precipitacions (Meteorologia), Sediment yield, Edafología, [SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics, PE&RC, 2502 Climatología, Drenatge, 6. Clean water, Weather types, Tipos de clima, Erosión, Precipitations (Meteorology), 2511 Ciencias del Suelo (Edafología), 2508.10 Precipitación, Seasonal analyses, Anticyclone, Erosió, Erosion, Drainage, Mediterrània occidental, Soil erosion--Climatic factors, Cuenca mediterranea, Runoff, 0207 environmental engineering, Environmental Science (miscellaneous), lcsh:QC851-999, complex mixtures, [SDV.EE.ECO]Life Sciences [q-bio]/Ecology, environment/Ecosystems, Atmospheric instability, Settore AGR/08 - Idraulica Agraria E Sistemazioni Idraulico-Forestali, Datasets, 0105 earth and related environmental sciences, Mediterraneanbasin, Hydrology, Water and Land Use, Rainfal, fungi, Sediment, Sedimentation and deposition, seasonal, 15. Life on land, Atmosfera -- Aspectes ambientals, Bodem, Water en Landgebruik, 13. Climate action, Enginyeria agroalimentària::Ciències de la terra i de la vida [Àrees temàtiques de la UPC], Soil erosion, Environmental science, Rainfall frequencies, Spatial variability, lcsh:Meteorology. Climatology, Clima, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Surface runoff
Abstract: Producción Científica, Rainfall is the key factor to understand soil erosion processes, mechanisms, and rates. Most research was conducted to determine rainfall characteristics and their relationship with soil erosion (erosivity) but there is little information about how atmospheric patterns control soil losses, and this is important to enable sustainable environmental planning and risk prevention. We investigated the temporal and spatial variability of the relationships of rainfall, runoff, and sediment yield with atmospheric patterns (weather types, WTs) in the western Mediterranean basin. For this purpose, we analyzed a large database of rainfall events collected between 1985 and 2015 in 46 experimental plots and catchments with the aim to: (i) evaluate seasonal differences in the contribution of rainfall, runoff, and sediment yield produced by the WTs; and (ii) to analyze the seasonal efficiency of the different WTs (relation frequency and magnitude) related to rainfall, runoff, and sediment yield. The results indicate two different temporal patterns: the first weather type exhibits (during the cold period: autumn and winter) westerly flows that produce the highest rainfall, runoff, and sediment yield values throughout the territory; the second weather type exhibits easterly flows that predominate during the warm period (spring and summer) and it is located on the Mediterranean coast of the Iberian Peninsula. However, the cyclonic situations present high frequency throughout the whole year with a large influence extended around the western Mediterranean basin. Contrary, the anticyclonic situations, despite of its high frequency, do not contribute significantly to the total rainfall, runoff, and sediment (showing the lowest efficiency) because of atmospheric stability that currently characterize this atmospheric pattern. Our approach helps to better understand the relationship of WTs on the seasonal and spatial variability of rainfall, runoff and sediment yield with a regional scale based on the large dataset and number of soil erosion experimental stations., Ministerio de Economía y Competitividad - FEDER (CGL2014 52135-C3-3-R; ESP2017-89463-C3-3-R; CGL2014-59946-R; CGL2015-65569-R; CGL2015-64284- C2-2-R; CGL2015-64284-C2-1-R; CGL2016-78075-P; GL2008-02879/BTE; LEDDRA 243857; RECARE-FP7; CGL2017-83866-C3-1-R y PCIN-2017- 061/AEI), Ministerio de Economía y Competitividad (FJCI-2017-33652), Organización para la Cooperación y el Desarrollo Económicos (TAD/CRP JA00088807), Junta de Castilla y León - FEDER (CLU-2018-04)

33. Inhibition of zygotic DNA repair: transcriptome analysis of the offspring in trout (Oncorhynchus mykiss)

Author: A Le Cam, María Paz Herráez, J Montfort, Cristina Fernández-Díez, Vanesa Robles, Serafín Pérez-Cerezales, Julien Bobe, Silvia González-Rojo, Department of Molecular Biology, Faculty of Biology, Campus Vegazana s/n, University of Leon, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), and Junta de Castilla y Leo´n (Spain) (project LE365A11-2) and the Spanish Ministry of Economy and Competitiveness (project AGL2011-27787)
Subjects: Male, Embryology, DNA Repair, Zygote, DNA repair, DNA damage, Embryonic Development, Poly(ADP-ribose) Polymerase Inhibitors, Biology, Real-Time Polymerase Chain Reaction, Transcriptome, chemistry.chemical_compound, Endocrinology, Animals, RNA, Messenger, Spermatogenesis, Gene, Cells, Cultured, Oligonucleotide Array Sequence Analysis, Reverse Transcriptase Polymerase Chain Reaction, Gene Expression Profiling, [SDV.BA]Life Sciences [q-bio]/Animal biology, Gene Expression Regulation, Developmental, Obstetrics and Gynecology, [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology, Embryo, Cell Biology, Base excision repair, Molecular biology, Cell biology, Reproductive Medicine, chemistry, Fertilization, Larva, Oncorhynchus mykiss, Biomarkers, DNA, DNA Damage
Abstract: Zygotic repair of the paternal genome is a key event after fertilization. Spermatozoa accumulate DNA strand breaks during spermatogenesis and can suffer additional damage by different factors, including cryopreservation. Fertilization with DNA-damaged spermatozoa (DDS) is considered to promote implantation failures and abortions, but also long-term effects on the progeny that could be related with a defective repair. Base excision repair (BER) pathway is considered the most active in zygotic DNA repair, but healthy oocytes contain enzymes for all repairing pathways. In this study, the effects of the inhibition of the BER pathway in the zygote were analyzed on the progeny obtained after fertilization with differentially DDS. Massive gene expression (GE; 61 657 unique probes) was analyzed after hatching using microarrays. Trout oocytes are easily fertilized with DDS and the high prolificacy allows live progeny to be obtained even with a high rate of abortions. Nevertheless, the zygotic inhibition of Poly (ADP-ribose) polymerase, upstream of BER pathway, resulted in 810 differentially expressed genes (DEGs) after hatching. DEGs are related with DNA repair, apoptosis, telomere maintenance, or growth and development, revealing a scenario of impaired DNA damage signalization and repair. Downregulation of the apoptotic cascade was noticed, suggesting a selection of embryos tolerant to residual DNA damage during embryo development. Our results reveal changes in the progeny from defective repairing zygotes including higher malformations rate, weight gain, longer telomeres, and lower caspase 3/7 activity, whose long-term consequences should be analyzed in depth.

34. Retraction Note: The role of nitric oxide during healing of trauma to the skeletal muscle.

Author: Filippin LI, Cuevas MJ, Lima E, Marroni NP, Gallego JG, and Xavier RM
Published: 2024
Full Text: View/download PDF

35. Changes in Motor Strategy and Neuromuscular Control During Balance Tasks in People with a Bimalleolar Ankle Fracture: A Preliminary and Exploratory Study.

Author: Salas-Gómez D, Barbado D, Sánchez-Juan P, Pérez-Núñez MI, Laguna-Bercero E, Lantarón-Juarez S, and Fernandez-Gorgojo M
Subjects: Humans, Male, Female, Adult, Middle Aged, Range of Motion, Articular physiology, Ankle Joint physiopathology, Ankle Joint physiology, Postural Balance physiology, Ankle Fractures surgery, Ankle Fractures physiopathology, Electromyography, Muscle, Skeletal physiopathology, Muscle, Skeletal physiology
Abstract: Ankle fractures can lead to issues such as limited dorsiflexion, strength deficits, swelling, stiffness, balance disorders, and functional limitations, which complicate daily activities. This study aimed to describe neuromuscular adaptations at 6 and 12 months post-surgery during static and dynamic balance tasks, specifically using the Y-Balance Test (YBT). Additionally, the relationship between neuromuscular patterns, balance, and musculoskeletal deficits was evaluated. In 21 participants (14 at 6 months and 21 at 12 months) with bimalleolar fractures, hip strength, ankle dorsiflexion, ankle functionality, and static and dynamic balance were assessed using electromyography of five lower limb muscles (tibialis anterior, peroneus longus, lateral gastrocnemius, biceps femoris, and gluteus medius). A significant interaction effect (limb × proximal [hip]-distal [ankle] muscle) (F = 30.806, p < 0.001) was observed in the anterior direction of the Y-Balance Test (YBT A ) at 6 months post-surgery. During the YBT A and YBT posteromedial (YBT PM ), it was found that a lower dorsiflexion range of movement was associated specifically at 6 months with greater activation of the lateral gastrocnemius. However, these differences tended to diminish by 12 months. These findings suggest that neuromuscular patterns differ between operated and non-operated limbs during the YBT A at 6 months post-surgery. The Y-Balance Test, particularly its anterior direction, effectively highlighted these neuromuscular changes. This is a preliminary study; further research is needed to explore these findings in depth.
Published: 2024
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36. ELLIS Study: Comparative Analysis of Excimer Laser Coronary Angioplasty and Intravascular Lithotripsy on Drug-Eluting Stent as Assessed by Scanning Electron Microscopy.

Author: Rivero-Santana B, Galán C, Pérez-Martínez C, Ibañez B, Pérez de Prado A, Fernández-Velasco M, Moreno R, and Jurado-Roman A
Abstract: Background: Stent underexpansion is a significant challenge in percutaneous coronary intervention, critically impacting patient outcomes. While excimer laser coronary angioplasty (ELCA) and intravascular lithotripsy (IVL) are increasingly used to address this issue, their full impact on the integrity of drug-eluting stents remains unclear, raising concerns about their safety and efficacy., Methods: This in vitro study assessed the effects of ELCA and IVL on the structural integrity of drug-eluting stents using scanning electron microscopy. Nine stents, 5 Onyx Frontier (with durable circumferential polymer coating) and 4 Cre8 (polymer-free), were implanted in a 3-dimensional coronary artery simulator following standardized protocols. After implantation, treatments with saline-ELCA, contrast-ELCA, IVL, and high-pressure balloon dilatation were applied. A comprehensive evaluation of the stent surface was performed at 60-fold magnification., Results: Scanning electron microscopy analysis revealed significant differences in polymer damage between the techniques. High-pressure balloon dilatation and contrast-ELCA exhibited substantial polymer fragmentation and detachment compared with IVL, saline-ELCA, and conventional dilatation. High-pressure balloon dilatation demonstrated the highest incidence of polymer shaving and overcoating. No significant alterations were observed in polymer-free stents, regardless of the technique used., Conclusions: IVL and saline-ELCA applied immediately after stent implantation produce minimal polymer damage, whereas high-pressure balloon dilatation and contrast-ELCA cause significant damage to the polymer coating. The integrity of polymer-free drug-eluting stent appears stable regardless of the technique used. Further research is needed to validate these findings and explore their clinical implications.
Published: 2024
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37. No evidence of improvements in energy metabolism after 1 week of nitrate and citrulline co-supplementation in elite rowers.

Author: Viribay A, Alcantara JMA, López I, Mielgo-Ayuso J, and Castañeda-Babarro A
Abstract: Purpose: Citrulline (CIT) and beetroot extract (BR) supplements positively impacts exercise performance in elite rowers. However, its influence on metabolic outcomes such as whole-body volumes of oxygen consumption (VO 2 ) and carbon dioxide production (VCO 2 ), substrate oxidation, energy expenditure (EE), and gross efficiency remains unknown. We studied the effects of 1 week of daily co-supplementation of 3.5 g BR (500 mg NO 3 - ) plus 6 g CIT on VO 2 and VCO 2 kinetics, substrate utilization, EE, and gross efficiency in elite male rowers compared to a placebo and to a BR supplementation., Methods: Twenty elite rowers participated in this randomized, double-blind, placebo-controlled crossover trial completing 1 week of supplementation in each group of study: Placebo (PLAG); BRG; and BR-CITG. Efficiency (70% VO 2max ) and performance (incremental maximal) tests were performed, and gas-exchange data were collected via indirect calorimetry., Results: Analysis of covariance (ANCOVA) showed no mean between-condition differences on respiratory exchange ratio (RER), EE, and gross efficiency in the efficiency test (all P > 0.06), and in the performance test (all P > 0.28). Moreover, in both tests no interaction Time × Supplement effects were observed for VO 2 , VCO 2 , RER, EE, substrate oxidation, and, gross efficiency (all P > 0.12)., Conclusion: After 1 week, no effects on energy metabolism and substrate utilization were observed after the daily co-ingestion of BR extract plus CIT supplement, therefore longer (> 7 days) and higher doses of supplementation might be needed to influence metabolism., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Published: 2024
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38. The Redefinition and Volumization of the Lip Area with Hyaluronic Acid: A Case Series.

Author: Ruiz N, Lopez RM, Marques R, and Fontenete S
Abstract: Background : The increasing popularity of non-surgical cosmetic enhancements for the lower face and perioral area, particularly through hyaluronic acid (HA) fillers, reflects the growing desire for improved lip volume and definition. This study showcases the effects of a specific HA filler on lip fullness, shape, and overall perioral rejuvenation. Methods : We conducted a retrospective single-site observational analysis of adult female patients treated with Genefill Soft Fill HA injections in the lips and perioral areas. Both patient and physician satisfaction were evaluated using the Likert scale and Global Aesthetic Improvement Scale (GAIS), respectively. The outcomes for natural appearance, volume, and durability were assessed using a five-point scale. The patients were followed up with for up to six months to monitor any adverse events. Results : The cohort included thirteen female patients with an average age of 55.3 ± 8.3 years. Approximately 1.2 ± 0.4 mL of filler was used per patient. The results indicate high satisfaction, with scores above 4 for naturalness, volume, and durability. Over 92% of patients reported a significant improvement in appearance. No moderate or severe adverse events were reported. Conclusions : Genefill Soft Fill HA filler is both effective and safe for enhancing lip esthetics, with high satisfaction rates among recipients and no significant adverse events observed.
Published: 2024
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39. Prevalence of suspected abuse of non-institutionalized older people treated in primary care. PRESENCIA study.

Author: Alonso-Moreno FJ, Llisterri Caro JL, Martínez Altarriba MC, Segura-Fragoso A, Martín-Sánchez V, Miravet Jiménez S, Velilla Zancada S, Martínez García FV, Micó Pérez RM, Cinza Sanjurjo S, and Sánchez Sánchez B
Subjects: Humans, Female, Male, Aged, Prevalence, Cross-Sectional Studies, Aged, 80 and over, Surveys and Questionnaires, Caregivers statistics & numerical data, Sex Factors, Primary Health Care, Elder Abuse statistics & numerical data, Elder Abuse diagnosis, Quality of Life
Abstract: Objectives: To determine the prevalence of suspected abuse of non-institutionalised elderly people and the associated variables., Patients and Method: Observational, descriptive, cross-sectional, multicentre study in patients aged 65 years or older, non-institutionalised, consecutively selected in primary care (PC). The EASI questionnaires (Suspected Elderly Abuse Index), the EAI questionnaire (Suspected Abuse Index in patients with cognitive impairment), the Barthel index, and the EUROQOL-5D questionnaire were used with patients, and the CASE questionnaire and the Zarit test were used with caregivers. Socio-demographic, health, and quality of life variables were analysed in all patients., Results: Eight hundred four patients were included, mean age 78.9±7.9 years, 58.3% women. The prevalence of suspected abuse was 11.3% (95% CI: 9.1%-13.9%). Suspected abuse was more frequent in women than in men (14.4% vs. 7.1%; odds ratio (OR)=1.97; 95% CI=1.1-3.4; p=0.016) and in those who lived with two or more people compared to those who lived alone (18.4% vs. 7.3%; OR=2.42; 95% CI=1.1-5.0; p=0.017). Among older patients, the lower their dependency, the lower the prevalence of suspected abuse (30.0% in highly dependent vs. 8.7% in non-dependent: p-trend=0.006); and the better the perceived health status, the lower the prevalence of suspected abuse (29.6% in poor health status vs. 6.9% in optimal health status; p-trend=<0.001). Among caregivers, the prevalence of suspected abuse was 20.4% (95% CI=12.8%-28.0%). A trend of higher prevalence of suspected abuse could be observed with higher scores on the CASE questionnaire (56.3% at high risk and 9.6% with no risk of abuse; p-trend=0.007). In the case of the ZARIT questionnaire with scores below 47, the prevalence of suspected abuse was 9.1%, and for scores above 55, it was 52.6% (p-trend<0.001)., Conclusions: The results of the PRESENCIA study show that approximately 1 in 10 patients aged ≥65 meet the criteria for suspected abuse. The probability of abuse increases in women, in patients with greater dependency and in patients with poorer perceived health status. Caregivers with greater overload and greater risk presented a greater suspicion of elder abuse., (Copyright © 2024 Sociedad Española de Médicos de Atención Primaria (SEMERGEN). Publicado por Elsevier España, S.L.U. All rights reserved.)
Published: 2024
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40. The impact of gambling advertising and marketing on online gambling behavior: an analysis based on Spanish data.

Author: García-Pérez Á, Krotter A, and Aonso-Diego G
Subjects: Humans, Spain, Longitudinal Studies, Gambling psychology, Advertising statistics & numerical data, Marketing, Internet
Abstract: Objectives: Online platforms have transformed gambling into a daily activity for many, raising concerns about its potential harm. Notably, marketing strategies play a crucial role in influencing gambling behaviors and normalizing gambling. This study aims to explore the relationship between monthly marketing expenditure by the gambling industry, the online amount of money bet, and the number of online accounts (active and new) in Spain. A secondary goal is to assess the impact of marketing restrictions under the Spanish Royal Decree 958/2020 on the relationship between marketing and online gambling behavior., Study Design: Longitudinal study., Methods: Data covering January 2013 to December 2023. Dependent variables included: new accounts, active accounts, gambler deposits, and the total money bet. Independent variables included: expenditure on advertising, bonuses, affiliate marketing, and sponsorship. A Seasonal Autoregressive Integrated Moving Average (SARIMA) model was employed to assess marketing's impact on online gambling behavior., Results: Findings show that investment in advertising (P ≤ 0.025), promotions (P < 0.001), and sponsorships (P ≤ 0.004) significantly increase the number of new and active accounts, deposits, and total money bet. For instance, it has been estimated that, for every €1 invested in bonuses and sponsorship, gamblers deposit €1.6 and €4 into their accounts, respectively. Moreover, the Spanish law regulating gambling advertising has seemingly weakened the link between marketing expenditure and gambling behavior, with the notable exception of bonuses, where the impact has intensified., Conclusions: These results underline the importance of ongoing monitoring and regulation of gambling behavior in Spain, emphasizing the need for strict adherence to regulations., (Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
Published: 2024
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41. Evaluation of the psychometric performance of the Spanish and Catalan versions of the patient reported experiences and Outcomes of Safety in Primary Care (PREOS-PC)-Compact questionnaire.

Author: Fiol-deRoque MA, Valderas JM, Arias de la Torre J, Serrano-Ripoll MJ, Gens-Barberà M, Sánchez-Freire E, Martín-Luján FM, Olry de Labry A, and Ricci-Cabello I
Subjects: Humans, Cross-Sectional Studies, Psychometrics, Reproducibility of Results, Patient Reported Outcome Measures, Primary Health Care
Abstract: Background: Patients provide a unique, irreplaceable, and essential perspective in evaluating patient safety. The suite of Patient Reported Experiences and Outcomes of Safety in Primary Care (PREOS-PC) tools are a notable exception to the scarcity of patient-reported patient safety measures. Full evaluation of their performance has only been attempted for the English version, thereby limiting its international applicability., Objectives: To assess the psychometric performance of the Spanish and Catalan versions of the PREOS-PC-Compact., Methods: Cross-sectional validation study. We used Classical Test Theory methods to examine scale score distribution, internal consistency, and construct validity; and Item Response Theory (IRT) methods to further explore construct validity., Results: 3287 patients completed the Spanish version, and 1007 the Catalan version. Similar results were obtained for both versions. Confirmatory Factor Analysis supported a single construct for each scale. The correlations between PREOS-PC-Compact scales and known group analysis suggested adequate construct validity (inconclusive for known groups at the provider level). All four multi-item scales demonstrated adequate internal consistency reliability (α > 0.7), which was only confirmed for test-retest reliability for 'Practice activation.' A sample between 60-90 patients per practice was estimated sufficient to produce scores with reliability > 0.7 for all scales except for harm scales. IRT models showed disordered thresholds for 'Practice activation' and 'Harm burden' but showed excellent fit after reducing the response categories., Conclusion: The Spanish and Catalan versions of the PREOS-PC-Compact are broadly valid and reliable tools to measure patient safety in Spanish primary care centres; confirmation of lower-than-expected test-rest reliability merits further examination .
Published: 2024
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42. Pharmacokinetic Pattern of Menbutone in Calves after Single Intravenous and Intramuscular Administration.

Author: Diez R, Rodriguez JM, Lopez C, de la Puente R, Sierra M, Diez MJ, Fernandez N, Garcia JJ, and Sahagun AM
Abstract: Menbutone is a choleretic agent currently used in Europe to treat digestive disorders in livestock and dogs. Pharmacokinetic parameters were established in 4-month Holstein calves after intravenous (IV) and intramuscular (IM) administration. The drug was administered to 12 animals (10 mg/kg) for both IV and IM routes following a crossover design. Plasma samples were collected at various time points over 24 h and analyzed by reverse-phase high-performance liquid chromatography with a photodiode-array detector, following a method validated according to European Medicines Agency guidelines. Pharmacokinetic parameters were calculated using compartmental and non-compartmental methods. Menbutone followed a two-compartment open model after IV injection, with a total clearance (Cl) of 71.9 ± 13.5 mL/h/kg, an elimination half-life (t ½β ) of 4.53 ± 2.45 h, and a volume of distribution at steady-state (V ss ) of 310.4 ± 106.4 mL/kg. Non-compartmental elimination half-life (t ½λ ) was 4.2 ± 1.1 h. After IM administration, drug pharmacokinetics was best described by a one-compartment open model. The peak plasma concentration (C max ) was 15.1 ± 4.3 µg/mL; the time to reach C max (t max ), 1.66 ± 0.55 h; and the mean absorption time (MAT), 2.50 ± 1.42 h. Absorption was high, with a fraction of the dose absorbed (F) of 83.5 ± 22.4%. Menbutone was rapidly eliminated from plasma for both routes of administration, with a fast and high IM bioavailability.
Published: 2024
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43. Anatomy education in low-resourced countries: What are challenges and effective and affordable educational strategies? A qualitative study.

Author: Chang Chan AYC, van Leeuwen M, Custers E, Bleys R, and Ten Cate O
Abstract: Purpose: With limited means, resource-deprived countries must find ways to organize education to meet standards. Few reports exist about anatomical education in LLMICs. This study explores how anatomy teaching is sustained in countries with few resources and which affordable educational strategies are applied to uphold quality., Methods: A mixed-methods study with anatomy teachers from public medical schools in low or lower-middle income countries (LLMICs) in Africa, Asia, Europe, and Latin-American was performed through survey via email combined with semi-structured online interview with teachers, exploring survey results at a deeper level., Results: LLMICs available to be surveyed, 13 and 8 were found to respond to a written survey and oral interview invitation, face significant teaching challenges, primarily due to lack of funds. These are faculty shortages (low salaries and high student-to-teacher ratio) and inadequate infrastructure (internet, electricity, poor classroom conditions). Solutions were associated with didactic strategies (social media, e-learning, image-based learning, applied anatomy), expanding teaching capacity with less qualified and part-time faculty, student-organized education, and self-financing (teaching resources subsidized by teachers and students). Which was triking was teacher commitment despite difficult circumstances. Teachers propose better faculty management, increased anatomy staff recruitment, and collaboration with other institutions., Conclusions: Anatomical education in LLMIC is forced to adapt to the socio-economic context, rather than to trends in medical education worldwide. These adaptations are supported mainly by the teachers 'commitment.'
Published: 2024
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44. Assessing Leg Blood Flow and Cardiac Output During Running Using Thermodilution.

Author: Leahy MG, Thompson KMA, Skattebo Ø, de Paz JA, Martin-Rincon M, Garcia-Gonzalez E, Galvan-Alvarez V, Boushel R, Hallén J, Burr JF, and Calbet JAL
Subjects: Humans, Male, Adult, Exercise Test methods, Thermodilution methods, Cardiac Output physiology, Running physiology, Leg blood supply, Leg physiology, Oxygen Consumption physiology, Regional Blood Flow physiology
Abstract: Cardiac output (Q̇ C ) and leg blood flow (Q̇ LEG ) can be measured simultaneously with high accuracy using transpulmonary and femoral vein thermodilution with a single-bolus injection. The invasive measure has offered important insight into leg hemodynamics and blood flow distribution during exercise. Despite being the natural modality of exercise in humans, there has been no direct measure of Q̇ LEG while running in humans. We sought to determine the feasibility of the thermodilution technique for measuring Q̇ LEG and conductance during high-intensity running, in an exploratory case study. A trained runner (30 years male) completed two maximal incremental tests on a cycle ergometer and motorized treadmill. Q̇ LEG and Q̇ C were determined using the single-bolus thermodilution technique. Arterial and venous blood were sampled throughout exercise, with continuous monitoring of metabolism, intra-arterial and venous pressure, and temperature. The participant reached a greater peak oxygen uptake (V̇O 2peak ) during running relative to cycling (74 vs. 68 mL/kg/min) with comparable Q̇ LEG (19.0 vs. 19.5 L/min) and Q̇ C (27.4 vs. 26.2 L/min). Leg vascular conductance was greater during high-intensity running relative to cycling (82 vs. 70 mL/min/mmHg @ ~80% V̇O 2peak ). The "beat phenomenon" was apparent in femoral flow while running, producing large gradients in conductance (62-90 mL/min/mmHg @ 70% V̇O 2peak ). In summary, we present the first direct measure of Q̇ LEG and conductance in a running human. Our findings corroborate several assumptions about Q̇ LEG during running compared with cycling. Importantly, we demonstrate that using thermodilution in running exercise can be completed effectively and safely., (© 2024 The Author(s). Scandinavian Journal of Medicine & Science In Sports published by John Wiley & Sons Ltd.)
Published: 2024
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45. Role of the Abcg2 transporter in plasma, milk, and tissue levels of the anthelmintic monepantel in mice.

Author: Gunes Y, Blanco-Paniagua E, Anlas C, Sari AB, Bakirel T, Ustuner O, and Merino G
Subjects: Animals, Female, Mice, Male, Mice, Knockout, Tissue Distribution, Tandem Mass Spectrometry, ATP Binding Cassette Transporter, Subfamily G, Member 2 metabolism, ATP Binding Cassette Transporter, Subfamily G, Member 2 genetics, Milk chemistry, Milk metabolism, Anthelmintics pharmacokinetics, Anthelmintics metabolism, Anthelmintics blood
Abstract: Breast cancer resistance protein/ATP-binding cassette subfamily G2 (BCRP/ABCG2) is an ATP-binding cassette efflux (ABC) transporter expressed in the apical membrane of cells in tissues, such as the liver, intestine, kidney, testis, brain, and mammary gland. It is involved in xenobiotic pharmacokinetics, potentially affecting the efficacy and toxicity of many drugs. In this study, the role of ABCG2 in parasiticide monepantel (MNP) and its primary metabolite, monepantel sulfone (MNPSO 2 )'s systemic distribution and excretion in milk, was tested using female and male wild-type and Abcg2 -/- mice. Liquid chromatography coupled with a tandem mass spectrometer (LC-MS/MS) was used for the analysis in a 10-min run time using positive-mode atmospheric pressure electrospray ionization (ESI+) and multiple reaction monitoring (MRM) scanning. For the primary metabolite tested, milk concentrations were 1.8-fold higher in wild-type mice than Abcg2 -/- female lactating mice (P = 0.042) after intravenous administration of MNP. Finally, despite the lack of a difference between groups, we investigated potential differences in MNP and MNPSO 2 's plasma and tissue accumulation levels between wild-type and Abcg2 -/- male mice. In this study, we demonstrated that MNPSO 2 milk levels were affected by Abcg2, with potential pharmacological and toxicological consequences, contributing to the undesirable xenobiotic residues in milk., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)
Published: 2024
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46. Post-mortem recrystallization of biogenic amorphous calcium carbonate guided by the inherited macromolecular framework.

Author: Stolarski J, Coronado I, Potocka M, Janiszewska K, Mazur M, Baronnet A, Cruz JA, Grauby O, and Meibom A
Subjects: Animals, Calcium Phosphates chemistry, Calcium Phosphates metabolism, Nephropidae metabolism, Nephropidae chemistry, Biomineralization, Calcium Carbonate chemistry, Calcium Carbonate metabolism, Fossils, Crystallization
Abstract: In contrast to abiotically formed carbonates, biogenetic carbonates have been observed to be nanocomposite, organo-mineral structures, the basic build-blocks of which are particles of quasi-uniform size (10-100 nm) organized into complex higher-order hierarchical structures, typically with highly controlled crystal-axis alignments. Some of these characteristics serve as criteria for inferring a biological origin and the state of preservation of fossil carbonate materials, and to determine whether the biomineralization process was biologically induced or controlled. Here we show that a calcium storage structure formed by the American lobster, a gastrolith initially consisting of amorphous calcium carbonate (ACC) and amorphous calcium phosphate (ACP), post-mortem can crystallize into (thus secondary) calcite with structural properties strongly influenced by the inherited organic matrix. This secondary calcite meets many structural criteria for biominerals (thus called the biomorphic calcite), but differs in trace element distributions (e.g., P and Mg). Such observations refine the capability to determine whether a fossil carbonates can be attributed to biogenic processes, with implications for the record of life on Earth and other terrestrial planets., (© 2024. The Author(s).)
Published: 2024
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47. [Prescription of drugs for smoking cessation. Retrospective analysis of the health area of Zamora].

Author: Majo García R, Fernández-García D, Fernández-Martínez MN, Espinosa Villoria MG, Amaro Rodríguez L, and Ruiz Cimarras MA
Abstract: Objective: To analyze the prescription of drugs to aid smoking cessation and to detect whether there are differences by age or sex., Design: Retrospective cohort study. SITE: Zamora Health Area., Participants: Persons with smoking cessation attempts employing drugs funded in the period from 2020 to 2023., Interventions: Request of pharmaceutical consumption of varenicline, bupropion and cytisine to the Pharmacy Information System of the Regional Health Management of Castilla y León., Main Measurements: Number of quit attempts per person, treatment drug, number of medication containers per attempt, year, age and sex. Descriptive and statistical analysis using SPSS© v. 20., Results: 2581 people tried to quit smoking with drugs, 2206 made one attempt and 375 made several attempts. Mean age was 50.7 years (95% CI: 50.2-51.1). No significant differences were found for age (P=.71) or sex (P=.74). There was a preference for prescribing varenicline over bupropion and low drug compliance, with only one container of medication being collected in about 50% of cases. A total of 1680 attempts were made to quit using cytisine in 2023, equivalent to 55.4% of the total number of treatment drugs used in the four years. The estimated cumulative incidence rate of drug withdrawal attempts in smokers between 18 and 65 years of age was 11.9%., Conclusions: The drug intervention had a low reach and poor compliance with the recommended treatment. It is essential to emphasize patient follow-up and drug adherence., (Copyright © 2024 The Author(s). Publicado por Elsevier España S.L.U. All rights reserved.)
Published: 2024
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48. The Effect of Passive Dehydration on Phase Angle and Body Composition: A Bioelectrical Impedance Analysis.

Author: Aburto-Corona JA, Calleja-Núñez JJ, Moncada-Jiménez J, and de Paz JA
Subjects: Humans, Male, Adult, Young Adult, Electric Impedance, Dehydration physiopathology, Body Composition, Weight Loss physiology, Body Water
Abstract: Bioelectrical impedance analysis (BIA) is a method used to estimate body composition, and it relies mainly on the body's water content. Insufficient body water can introduce bias to body composition scores., Purpose: To determine the effect of body weight loss elicited by passive dehydration on body composition scores, including phase angle (PhA)., Methods: Twenty-five euhydrated apparently healthy and physically active men's (age = 22.6 ± 3.3 yr.; body mass = 76.7 ± 15.9 kg; height = 172.0 ± 6.3 cm) body composition variables and PhA were measured before and after sitting quietly for 5 h in a controlled environment (26.6 ± 1.7 °C, 72 ± 4.9%RH)., Results: It was found that five hours of passive dehydration caused a loss in body weight (Δ = 0.76 ± 0.34 kg, p < 0.05) and a decrease in body fat estimation (Δ = 0.90 ± 0.87 kg, p < 0.001). Additionally, an increase in ECW (Δ = 0.12 ± 0.30 L, p < 0.021) and PhA (Δ = 0.10 ± 0.15°, p < 0.005) was observed., Conclusion: Body weight loss due to passive dehydration decreased BIA-derived fat mass, and increased extracellular water and PhA in physically active and apparently healthy men. Nonetheless, these changes had a negligible effect on the accuracy of the equipment, rendering them clinically insignificant.
Published: 2024
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49. The Influence of Anthropometric Variables on the Performance of Elite Traditional Rowers.

Author: Castañeda-Babarro A, León-Guereño P, Viribay A, Gutiérrez-Santamaría B, López I, and Mielgo-Ayuso J
Abstract: Athletes' anthropometry, and especially their body composition, plays an important role in sport performance in general and in Trainera rowing in particular. Rowers' anthropometric and performance profiles may vary according to their position in the boat. The objectives of this study were to investigate the relationship between anthropometry, physical performance, physiological variables, and elite male rowers' boat positions. Twenty elite male traditional rowers were assessed and categorized according to their boat position: either in the middle of the boat (M) ( n = 9) or in the bow and stern positions (BS) ( n = 11). Anthropometric measurements and body composition were obtained for each rower, and physical performance was measured by a 45-s supramaximal rowing test and a VO 2max incremental test on a Concept II rowing ergometer. The results showed that the rowers in the middle were taller (186.6 ± 4.9 cm), and significant differences were also found between the two groups according to body mass (BS 72.3 ± 3.8 vs. M 85.4 ± 4.3) and peak power (BS 641.5 ± 84 vs. M 737 ± 47.1), mean power (BS 538.5 ± 48.4 vs. M 604.1 ± 42.3), and physiological parameters ( p < 0.05), VO 2max (BS 66.5 ± 4.9 vs. M 59.3 ± 6.7). It can be concluded that height could be associated with elite rowers' performance and that a lower body mass index is related to better performance in bow and stern positions.
Published: 2024
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50. Factors that affect the health of immigrants: Qualitative meta synthesis.

Author: Villar-Bustos C, Quiroga Sánchez E, and Andina-Díaz E
Subjects: Humans, Female, Male, Health Status, Qualitative Research, Emigrants and Immigrants psychology
Abstract: Background: Migration has challenged society. Most people who move do so for economic reasons, but others move for more tragic reasons. The proportion of female migrants was slightly higher than that of male migrants, partly due to the longer life expectancy of women and the higher demand for female migrants in care-related Jobs. The process may affect migrants' health, particularly in countries where healthcare is associated with high economic costs or insurance availability. A global systematic review of qualitative studies with meta-synthesis was conducted. The results can be used to support health policy and clinical practice., Objective: To describe how migrants perceive and experience the process of migrating and how it affects their health., Search Strategy: Databases consulted were Medline, PsychInfo, Cuiden, Cinahl, WOS, Scopus, Social Science Database, and Epistemonikos. Thirty-four articles were selected for final meta-synthesis., Inclusion Criteria: All qualitative primary studies were included that describe the experiences or perceptions of migrants and refugees over 18 years that talk about their migration process and the impact on their health; written in English or Spanish between 2016 and 2021. Articles referring to second generations and those dealing with pathologies that pre-date the migration process were excluded., Data Extraction and Synthesis: The COREQ and JBI templates were used as quality criteria. Studies mostly used a phenomenological methodology and in-depth interviews, both individual and group, were used for data collection and narrative synthesis., Main Results: Uncertainty emerges as a main category. Three other interrelated themes have a direct impact on migrants' health: Language, Social Networks and Work. There are several conditions in each of these that have a positive or negative impact on health. The gender condition appears in both work and social networks, positively and negatively., Discussion and Conclusions: Health would be improved by having a stable job, which would facilitate access to health resources. Social networks and language are facilitators of access to a better job, but not the only condition. From a gender perspective, social networks can become a source of health problems, especially for women. The process of migration places women in a position of vulnerability due to the difficulties of reconciling family and work life. Job insecurity, workload, loss of family life or social isolation increase hopelessness and anxiety, leading to health problems., Public or Patient Contribution: As an academic review study, no patient contribution was required, and this study serves as a theoretical framework for more in-depth research that will work with migrant populations. As a public contribution, this work provides evidence of the need to improve access to health for some populations, in line with the Sustainable Development Goals (SDGs) set for 2030., (© 2024 The Authors. Public Health Nursing published by Wiley Periodicals LLC.)
Published: 2024
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