1. Saccharothrix algeriensis NRRL B-24137, the first non-Streptomyces actinobacterium, produces holomycin after cystine feeding
- Author
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Rabiâa Merrouche, Amine Yekkour, Noureddine Bouras, Abdelghani Zitouni, Nasserdine Sabaou, Yannick Coppel, Florence Mathieu, Centre National de la Recherche Scientifique - CNRS (FRANCE), Ecole normale supérieure de Kouba (ALGERIA), Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE), Université Ghardaia (ALGERIA), Université Toulouse III - Paul Sabatier - UT3 (FRANCE), Institut National de la Recherche Agronomique d'Algérie - INRAA (ALGERIE), Laboratoire de Chimie de Coordination - LCC (Toulouse, France), Laboratoire de Génie Chimique - LGC (Toulouse, France), École normale supérieure - Kouba-Alger (ENS Kouba-Alger), Institut National de la Recherche Agronomique d'Algérie (INRAA), Laboratoire de chimie de coordination (LCC), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie de Toulouse (ICT-FR 2599), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), School of Physics and Materials Engineering, Monash University [Clayton], Laboratoire de Génie Chimique (LGC), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées, Institut de Chimie de Toulouse (ICT), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), and Université de Toulouse (UT)
- Subjects
Dithiolopyrrolone antibiotics ,Lactams ,medicine.drug_class ,Klebsiella pneumoniae ,Matériaux ,Antibiotics ,Microbial Sensitivity Tests ,Antimicrobial activity ,medicine.disease_cause ,Biochemistry ,Microbiology ,Streptomyces ,Actinobacteria ,03 medical and health sciences ,Holomycin ,Anti-Infective Agents ,Genetics ,medicine ,L-cystine ,Molecular Biology ,Escherichia coli ,030304 developmental biology ,0303 health sciences ,Bacteria ,biology ,Bactériologie ,030306 microbiology ,Chemistry ,Fungi ,General Medicine ,Antimicrobial ,biology.organism_classification ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,Culture Media ,3. Good health ,Staphylococcus aureus ,Fermentation ,Dithiolopyrrolone antibiotics - Holomycin - Saccharothrix algeriensis NRRL B-24137 - L-cystine - Antimicrobial activity ,Cystine ,Saccharothrix algeriensis NRRL B-24137 - Abstract
International audience; Saccharothrix algeriensis NRRL B-24137 is an actinobacterium isolated from Algerian Saharan soil. This strain has the ability to produce several dithiolopyrrolone antibiotic derivatives depending on the precursors added to the culture medium. This group of antibiotics is known for their potent antimicrobial and anticancer activities. Holomycin is a member of the dithiolopyrrolone group of antibiotics, and has already been isolated from several species of actinobacteria belonging to the genus Streptomyces and also from some Gram-negative bacteria. In this study, holomycin was produced for the first time in the culture broth of a non-Streptomyces actinobacteria. This antibiotic was induced by adding 5 mM of L-cystine as precursor to the semi-synthetic fermentation broth of Sa. algeriensis NRRL B-24137 and then fully identified after HPLC purification. The minimum inhibitory concentrations (MIC) of holomycin were determined against several pathogenic microorganisms, including Escherichia coli ATCC 10536 Klebsiella pneumoniae CIP 82.91, Listeria monocytogenes CIP 82110, Staphylococcus aureus CIP 7625, Aspergillus carbonarius M333, Fusarium culmorum FC1, Candida albicans IPA 200. This antibiotic showed a broad-spectrum antimicrobial activity, inhibiting a variety of Gram-positive and Gram-negative bacteria, and micro-fungi.
- Published
- 2020
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