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Made available in DSpace on 2018-11-26T17:16:32Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-01-15 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) The Abrolhos Bank is an area of high ecological, socio-economic importance and harbour the richest and most extensive coral reefs in the South Atlantic. Here we report the discovery of shallow (12-25 m depth) reef complex with ten large biogenic structures, intermediate between the typical mushroom-shaped pinnacles of the northern Abrolhos Bank (17 degrees-18 degrees S) and the small patch reefs found on the central/southern coast of the Espirito Santo State (19 degrees-20 degrees S). The newly discovered reefs harbour a relatively rich and abundant reef community, with 73 fish and 14 benthic cnidarian species, including endangered and commercially important ones. We discuss on urgent needs of properly mapping and understanding the ecological functioning of this reef system. Information provided here is a baseline for future impact evaluations, particularly considering the recent worst environmental disaster of Brazil from a dam collapse in Doce river that affected the region. (C) 2016 Elsevier Ltd. All rights reserved. Univ Fed Espirito Santo, Dept Oceanog & Ecol, Ave Fernando Ferrari,514 Goiabeiras, Vitoria 29075910, ES, Spain Assoc Ambiental Voz Nat, Vitoria, ES, Spain Univ Fed Estado Rio de Janeiro, Programa Posgrad Ciencias Biol Biodiversidade Neo, Rio De Janeiro, RJ, Brazil Inst Meros Brasil, Curitiba, PR, Brazil Calif Acad Sci, San Francisco, CA 94118 USA Univ Estadual Paulista, Dept Morfol, Inst Biociencias, Botucatu, SP, Brazil Inst COMAR Conservacao Marinha Brasil, Joinville, SC, Brazil Univ Fed Paraiba, Ctr Ciencias Aplicadas & Educ, Rio Tinto, PB, Brazil Univ Fed Espirito Santo, Ctr Univ Norte Espirito Santo, Sao Mateus, ES, Brazil Univ Calif Santa Cruz, Ecol & Evolutionary Biol Dept, Santa Cruz, CA 95064 USA Univ Estadual Paulista, Dept Morfol, Inst Biociencias, Botucatu, SP, Brazil CNPq: GDE 202475/ 2011-5 CNPq: 54515130/2011

BioMarin Pharmaceutical Inc. Shire Genzyme BioMarin Background: Mucopolysaccharidosis type VI (MPS VI) is a progressive, chronic and multisystem lysosomal storage disease with a wide disease spectrum. Clinical and biochemical improvements have been reported for MPS VI patients on enzyme replacement therapy (ERT) with rhASB (recombinant human arylsulfatase B; galsulfase, Naglazyme (R), BioMarin Pharmaceutical Inc.), making early diagnosis and intervention imperative for optimal patient outcomes. Few studies have included children younger than five years of age. This report describes 34 MPS VI patients that started treatment with galsulfase before five years of age.Methods: Data from patients who initiated treatment at

Made available in DSpace on 2018-11-26T16:40:42Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-04-01. Added 1 bitstream(s) on 2021-07-15T15:01:27Z : No. of bitstreams: 1 S1415-47572016000200257.pdf: 1734107 bytes, checksum: 78a80ed0809fe071b511960c5a72d6cb (MD5) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Brazilian green propolis is a resinous substance prepared by bees from parts of the plant Baccharis dracunculifolia. As it possess several biological properties, this work assessed the cytotoxic/anticytotoxic, genotoxic/antigenotoxic and mutagenic/antimutagenic potential of ethanolic extracts of Brazilian green propolis (EEGP) and of B. dracunculifolia (EEBD), by means of the Allium cepa test system. The effects were evaluated by assessing the chromosomal aberrations (CA) and micronuclei (MN) frequencies on meristematic and F1 generation cells from onion roots. Chemical analyses performed with the extracts showed differences in flavonoid quality and quantity. No genotoxic or mutagenic potential was detected, and both extracts were capable of inhibiting cellular damage caused by methyl methanesulfonate (MMS) treatment, reducing the frequencies of CA and MN. By these data, we can infer that, independent of their flavonoid content, the extracts presented a protective effect in A. cepa cells against the clastogenicity of MMS. Univ Estadual Paulista, Inst Biociencias, Dept Biol, Rio Claro, SP, Brazil Univ Fed Espirito Santo, Centro Ciencias Saude, Dept Ciencias Farmaceut, Vitoria, ES, Spain Univ Estadual Paulista, Ctr Estudo Insetos Sociais, Rio Claro, SP, Brazil Univ Estadual Paulista, Inst Biociencias, Dept Biol, Rio Claro, SP, Brazil Univ Estadual Paulista, Ctr Estudo Insetos Sociais, Rio Claro, SP, Brazil

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) State Agency for the Development of Science and Technology (FAPES/Universal) Background: the clipping of an artery supplying one of the two kidneys (2K1C) activates the renin-angiotensin (Ang) system (RAS), resulting in hypertension and endothelial dysfunction. Recently, we demonstrated the intrarenal beneficial effects of sildenafil on the high levels of Ang II and reactive oxygen species (ROS) and on high blood pressure (BP) in 2K1C mice. Thus, in the present study, we tested the hypothesis that sildenafil improves endothelial function in hypertensive 2K1C mice by improving the NO/ROS balance.Methods: 2K1C hypertension was induced in C57BL/6 mice. Two weeks later, they were treated with sildenafil (40 mg/kg/day, via oral) or vehicle for 2 weeks and compared with sham mice. At the end of the treatment, the levels of plasma and intrarenal Ang peptides were measured. Endothelial function and ROS production were assessed in mesenteric arterial bed (MAB).Results: the 2K1C mice exhibited normal plasma levels of Ang I, II and 1-7, whereas the intrarenal Ang I and II were increased (similar to 35% and similar to 140%) compared with the Sham mice. Sildenafil normalized the intrarenal Ang I and II and increased the plasma (similar to 45%) and intrarenal (+15%) Ang 1-7. the 2K1C mice exhibited endothelial dysfunction, primarily due to increased ROS and decreased NO productions by endothelial cells, which were ameliorated by treatment with sildenafil.Conclusion: These data suggest that the effects of sildenafil on endothelial dysfunction in 2K1C mice may be due to interaction with RAS and restoring NO/ROS balance in the endothelial cells from MAB. Thus, sildenafil is a promising candidate drug for the treatment of hypertension accompanied by endothelial dysfunction and kidney disease. Univ Fed Espirito Santo, Hlth Sci Ctr, Lab Translat Physiol, Vitoria, ES, Brazil Emescam Sch Hlth Sci, Vitoria, ES, Brazil Universidade Federal de São Paulo, Dept Med, Div Nephrol, São Paulo, Brazil Univ Fed Espirito Santo, Hlth Sci Ctr, Pharmaceut Sci Grad Program, Vitoria, ES, Brazil Univ Fed Paraiba, Hlth Sci Ctr, Dept Physiol & Pathol, BR-58059900 Joao Pessoa, PB, Brazil UVV, Pharmaceut Sci Grad Program, Vila Velha, ES, Brazil Fed Inst Educ Sci & Technol IFES, Vila Velha, ES, Brazil Universidade Federal de São Paulo, Dept Med, Div Nephrol, São Paulo, Brazil CNPq: 302582/2011-8 CNPq: 476525/2012-8 CNPq: 305188/2012-7 CNPq: 473177/2013-7 State Agency for the Development of Science and Technology (FAPES/Universal): 012/2011 State Agency for the Development of Science and Technology (FAPES/Universal): 54498465 CNPq: 012/2009 Web of Science

Tuberculosis Research Unit at Case Western Reserve University U. S. National Institutes of Allergy and Infectious Diseases National Institutes of Health and Human Services Fundacao de Apoio a Ciencia e Tecnologia do Espirito Santo Low-colony-number counts on solid media are considered characteristic of cross-contamination, although they are normally observed in true-positive cultures from some groups of patients. the aim of this study was to evaluate low-yield growth cultures as a microbiological marker for cross-contamination. We evaluated 106 cultures with < 15 colonies from 94 patients, and the proportions of false-positive cultures were 0.9% per sample and 1.1% per patient, which indicates that low-yield growth is not a reliable marker of cross-contamination. Univ Fed Espirito Santo, Ctr Ciencias Saude, Nucleo Doencas Infecciosas, Vitoria, ES, Brazil Universidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, São Paulo, Brazil Case Western Reserve Univ, Sch Med, Dept Med, TB Res Unit, Cleveland, OH 44106 USA Univ Hosp Case Med Ctr, Cleveland, OH USA Univ Arkansas Med Sci, Dept Pathol, Little Rock, AR 72205 USA Universidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, São Paulo, Brazil National Institutes of Health and Human Services: NO1-AI95383 National Institutes of Health and Human Services: HHSN266200700022C/NO1-AI-70022 Fundacao de Apoio a Ciencia e Tecnologia do Espirito Santo: 37396285/2007 Web of Science

Background Childhood diarrheal diseases remain highly endemic in developing areas of Brazil. The importance of Escherichia coli among children with diarrhea in these areas was unknown. This study determined the prevalence of different E. coli categories in symptomatic and asymptomatic children from low socioeconomic level rural communities in southeastern Brazil. Methods A total of 560 stool samples were collected from 141 children with diarrhea (< 10 years) and 419 apparently healthy controls who resided in 23 communities. E. coli isolates (n = 1943) were subjected to two multiplex PCRs developed for the detection of enteropathogenic E. coli (EPEC), enteroaggregative E. coli (EAEC), diffusely adherent E. coli (DAEC), enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC), and Shiga toxin-producing E. coli (STEC). Strains were also examined for the presence of EPEC, EAEC, and DAEC by assays of adhesion to HEp-2 cells and by hybridization with specific DNA probes. Results Diarrheagenic E. coli strains were isolated from 253 (45.2%) children, and were associated with diarrhea in children aged < 5 years (p < 0.001). EAEC (20.9%), DAEC (11.6%), EPEC (9.3%) were the most frequent pathotypes, followed by ETEC (2.7%), EIEC (0.5%), and STEC (0.2%). Depending of the assay, EPEC, EAEC, and DAEC (collectively termed enteroadherent E. coli) strains were isolated in 45% to 56% of diarrhea cases, a significantly higher incidence than in controls (P < 0.05). Individually, only DAEC showed significant association with diarrhea (p < 0.05), particularly in children aged 2–5 years. Conclusion This study indicates that enteroadherent E. coli is an important cause of diarrhea in children living in low socioeconomic level communities in southeastern Brazil. Our results reveal that the PCR1 assay is an excellent tool for the identification of EAEC and DAEC.

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Background the present study addresses the hypothesis that the activity of dipeptidyl peptidase IV (DPPIV), an enzyme that inactivates peptides that possess cardioprotective actions, correlates with adverse outcomes in heart failure (HF). the therapeutic potential of DPPIV inhibition in preventing cardiac dysfunction is also investigated.Methods and Results Measurements of DPPIV activity in blood samples obtained from 190 patients with HF and 42 controls demonstrated that patients with HF exhibited an increase of approximate to 130% in circulating DPPIV activity compared with healthy subjects. Furthermore, an inverse correlation was observed between serum DPPIV activity and left ventricular (LV) ejection fraction in patients with HF. Similarly, radiofrequency LV ablation-induced HF rats displayed higher DPPIV activity in the plasma (approximate to 50%) and heart tissue (approximate to 3.5-fold) compared with sham-operated rats. Moreover, positive correlations were observed between the plasma DPPIV activity and LV end-diastolic pressure and lung congestion. Two days after surgery, 1 group of LV ablation-induced HF rats was treated with the DPPIV inhibitor sitagliptin (40 mg/kg BID) for 6 weeks, whereas the remaining rats were administered water. Hemodynamic measurements demonstrated that radiofrequency LV-ablated rats treated with sitagliptin exhibited a significant attenuation of HF-related cardiac dysfunction, including LV end-diastolic pressure, systolic performance, and chamber stiffness. Sitagliptin treatment also attenuated cardiac remodeling and cardiomyocyte apoptosis and minimized pulmonary congestion.Conclusions Collectively, the results presented herein associate circulating DPPIV activity with poorer cardiovascular outcomes in human and experimental HF. Moreover, the results demonstrate that long-term DPPIV inhibition mitigates the development and progression of HF in rats. Univ São Paulo, Heart Inst InCor, Sch Med, BR-05403900 São Paulo, Brazil Univ Fed Espirito Santo, Dept Physiol Sci, Vitoria, Brazil Univ São Paulo, Universidade Federal de São Paulo, Dept Physiol, BR-05403900 São Paulo, Brazil Univ São Paulo, Universidade Federal de São Paulo, Dept Physiol, BR-05403900 São Paulo, Brazil FAPESP: 07/52945-8 FAPESP: 09/54225-8 FAPESP: 12/10146-0 CNPq: 479953/2011-2 Web of Science

Sex hormones modulate the action of both cytokines and the renin-angiotensin system. However, the effects of angiotensin I-converting enzyme (ACE) on the proinflammatory and anti-inflammatory cytokine levels in male and female spontaneously hypertensive rats (SHR) are unclear. We determined the relationship between ACE activity, cytokine levels and sex differences in SHR. Female (F) and male (M) SHR were divided into 4 experimental groups each (n = 7): sham + vehicle (SV), sham + enalapril (10 mg/kg body weight by gavage), castrated + vehicle, and castrated + enalapril. Treatment began 21 days after castration and continued for 30 days. Serum cytokine levels (ELISA) and ACE activity (fluorimetry) were measured. Male rats exhibited a higher serum ACE activity than female rats. Castration reduced serum ACE in males but did not affect it in females. Enalapril reduced serum ACE in all groups. IL-10 (FSV = 16.4 +/- 1.1 pg/mL; MSV = 12.8 +/- 1.2 pg/mL), TNF-alpha (FSV = 16.6 +/- 1.2 pg/mL; MSV = 12.8 +/- 1 pg/mL) and IL-6 (FSV = 10.3 +/- 0.2 pg/mL; MSV = 7.2 +/- 0.2 pg/mL) levels were higher in females than in males. Ovariectomy reduced all cytokine levels and orchiectomy reduced IL-6 but increased IL-10 concentrations in males. Castration eliminated the differences in all inflammatory cytokine levels (IL-6 and TNF-alpha) between males and females. Enalapril increased IL-10 in all groups and reduced IL-6 in SV rats. in conclusion, serum ACE inhibition by enalapril eliminated the sexual dimorphisms of cytokine levels in SV animals, which suggests that enalapril exerts systemic anti-inflammatory and anti-hypertensive effects. Univ Fed Espirito Santo, Dept Ciencias Fisiol, BR-29042755 Vitoria, ES, Brazil Inst Fed Espirito Santo, Vitoria, ES, Brazil Ctr Univ Vila Velha, Dept Farm, Vila Velha, ES, Brazil Universidade Federal de São Paulo, Escola Paulista Med, Dept Biofis, São Paulo, Brazil Universidade Federal de São Paulo, Escola Paulista Med, Dept Biofis, São Paulo, Brazil Web of Science

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Inoue BH, dos Santos L, Pessoa TD, Antonio EL, Pacheco BPM, Savignano FA, Carraro-Lacroix LR, Tucci PJF, Malnic G, Girardi ACC. Increased NHE3 abundance and transport activity in renal proximal tubule of rats with heart failure. Am J Physiol Regul Integr Comp Physiol 302: R166-R174, 2012. First published October 26, 2011; doi:10.1152/ajpregu.00127.2011.-Heart failure (HF) is associated with a reduced effective circulating volume that drives sodium and water retention and extracellular volume expansion. We therefore hypothesized that Na(+)/H(+) exchanger isoform 3 (NHE3), the major apical transcellular pathway for sodium reabsorption in the proximal tubule, is upregulated in an experimental model of HF. HF was induced in male rats by left ventricle radiofrequency ablation. Sham-operated rats (sham) were used as controls. At 6 wk after surgery, HF rats exhibited cardiac dysfunction with a dramatic increase in left ventricular end-diastolic pressure. By means of stationary in vivo microperfusion and pH-dependent sodium uptake, we demonstrated that NHE3 transport activity was significantly higher in the proximal tubule of HF compared with sham rats. Increased NHE3 activity was paralleled by increased renal cortical NHE3 expression at both protein and mRNA levels. in addition, the baseline PKA-dependent NHE3 phosphorylation at serine 552 was reduced in renal cortical membranes of rats with HF. Collectively, these results suggest that NHE3 is upregulated in the proximal tubule of HF rats by transcriptional, translational, and posttranslational mechanisms. Enhanced NHE3-mediated sodium reabsorption in the proximal tubule may contribute to extracellular volume expansion and edema, the hallmark feature of HF. Moreover, our study emphasizes the importance of undertaking a cardiorenal approach to contain progression of cardiac disease. Univ São Paulo, Heart Inst InCor, Sch Med, BR-05403900 São Paulo, Brazil Univ Fed Espirito Santo, Dept Physiol Sci, Vitoria, ES, Spain Univ São Paulo, Dept Physiol & Biophys, Inst Biomed Sci, BR-05403900 São Paulo, Brazil Universidade Federal de São Paulo, Dept Physiol, São Paulo, Brazil Universidade Federal de São Paulo, Dept Physiol, São Paulo, Brazil Web of Science

ObjectiveTo estimate the prevalence of musculoskeletal manifestations in the population of Vitoria, Brazil, using the COPCORD questionnaire.MethodsThis was a cross-sectional study of 578 people with an age range of 18-65. The sample was probabilistic and stratified according to sex, age and socio-economic class, proportional to the 2000 demographic census data of the Brazilian Institute of Geography and Statistics - IBGE. The COPCORD core questionnaire was used for all subjects, and a rheumatologist evaluated those patients who presented pain and/or functional disability. Laboratory tests and radiographs were carried out in some patients to confirm the diagnosis.ResultsOne hundred and seventy-six patients (30.4%) with a mean age of 41 (SD 13) and predominantly female had pain. Among the patients with pain, 23.2% were unable to do daily living activities. One hundred and thirty (73.9%) were evaluated by a rheumatologist.ConclusionThe prevalence of musculoskeletal manifestations evaluated by the COPCORD questionnaire was 30.4%. Univ Fed Espirito Santo, Serv Reumatol, Dept Rheumatol, BR-29055131 Vitoria, ES, Brazil Univ Fed Espirito Santo, Dept Stat, BR-29055131 Vitoria, ES, Brazil Univ Fed Sao Paulo, Dept Rheumatol, Sao Paulo, Brazil Univ Fed Sao Paulo, Dept Rheumatol, Sao Paulo, Brazil Web of Science

The IS6110 repetitive element is present in multiple copies in most Mycobacterium tuberculosis complex bacteria, except for Mycobacterium bovis strains, which usually contain a single copy of IS6110 located on a 1·9 kb PvuII fragment of the direct repeat region. IS6110 transposition can disrupt coding regions and is a major force of genomic variation. In a previous work it was demonstrated that phospholipase C genes are preferential loci for IS6110 transposition in M. tuberculosis clinical strains. Bacterial phospholipase C enzymes participate in pathogenic mechanisms used by different organisms, and have been implicated in intracellular survival, cytolysis and cell-to-cell spread. Four phospholipase C genes (plcA, plcB, plcC and plcD) were detected in the genomes of M. tuberculosis, Mycobacterium africanum, Mycobacterium microti and ‘Mycobacterium canettii’. M. bovis and the vaccine strain M. bovis Bacillus Calmette–Guérin contain only the plcD gene. In the present work, the existence of IS6110 insertions within plcD, the unique phospholipase C gene of M. bovis, has been investigated by PCR, Southern blot hybridization and sequencing analysis. In 18 (7·3 %) of 245 isolates analysed, the plcD gene was interrupted by the insertion of one copy of IS6110, which in all cases was transposed in the same orientation and at the same position, 1 972 894, relative to the genome of M. bovis AF2122/97. These 18 isolates were distributed in 6 different spoligotype patterns and contained 4 to 8 IS6110 copies. In contrast, strains showing an intact plcD gene contained one (87 %), two (9·4 %) or three (2·4 %) IS6110 copies, and only a single isolate (1·2 %) had four IS6110 copies. The implications of plcD gene disruption in M. bovis have not been fully investigated, but no differences in the organ distribution of the disease were detected when animals infected with strains from the same spoligotype patterns bearing plcD : : IS6110 and intact plcD were compared.

Albert Einstein Medicina Diagnostica Alka Tecnologia Amaral Costa Laboratorio Conselho Federal de Biomedicina DASA Euroimmun Brasil Grupo Fleury Hemagen Medivax Olimpus Padrao Laboratorio Clinic Pontificia Universidade Catolica de Goias - PUC-Goias Sociedade Brasileira de Patologia Clinica e Medicina Laboratorial Sociedade Brasileira de Reumatologia Thermo Scientific Wama Diagnostica Werfen Group - Werfen Medical Hermes Pardini Conselho Regional de Biomedicina - 3a Regiao Objetivo: O IV Consenso Brasileiro para Pesquisa de Autoanticorpos em Células HEp-2 (FAN) realizado em Vitória (ES), no dia 18 de setembro de 2012, objetivou discutir estratégias e recomendações relacionadas ao procedimento técnico, à padronização e à interpretação dos resultados da pesquisa de autoanticorpos em células HEp-2. Métodos: Participaram do evento 23 pesquisadores e especialistas de Universidades e laboratórios brasileiros. Foram abordados diferentes tópicos, discutidos amplamente a fim de se estabelecer recomendações específicas. Resultados e conclusão: O IV Consenso integrou à árvore de decisão o padrão citoplasmático em Anéis e Bastões, o padrão nuclear pontilhado Quasi-homogêneo (QH) e o padrão misto CENP-F. Discutiu-se ainda a necessidade de atenção para a classificação do padrão misto relacionado à presença de anticorpos anti-DNA topoisomerase I (Scl-70), compreendendo os componentes nuclear pontilhado fino, nucleolar homogêneo, NOR na placa metafásica e citoplasmático pontilhado fino. Foram sugeridas diretrizes para o controle de qualidade do teste, diluição de triagem e diluição de esgotamento, e foi emitido alerta quanto à necessidade de atenção em relação à heterogeneidade de substratos disponíveis no mercado e a utilização de metodologias automatizadas para detecção de autoanticorpos. Objective: the Fourth Brazilian Consensus for Autoantibodies Screening in HEp-2 Cells (ANA) was held in Vitoria, Espirito Santo, and aimed to discuss strategies and recommendations about the technique, standardization, interpretation and quality control of the indirect immunofluorescence reaction on HEp-2 cells.Methods: Twenty three ANA experts from university centers and private laboratories in different areas from Brazil discussed and agreed upon recommendations for the fourth edition of the Brazilian Consensus for Autoantibodies Screening in HEp-2 Cells.Results and conclusion: the 4th ANA Consensus included three novel patterns into the existing algorithm (cytoplasmic Rods and Rings, nuclear Quasi-homogeneous, and CENP-F). Emphasis was given to the need of attention in describing the peculiar mixed pattern elicited by anti-DNA topoisomerase I (Scl-70) autoantibodies, comprising nuclear fine specked, nucleolar homogeneous pattern, NOR staining in metaphase plates, and cytoplasmic fine speckled patterns. the group also emphasized the need for continuous quality control in indirect immunofluorescence assays, the establishment of screening dilutions, as well as conjugate titration. An alert was made regarding the heterogeneity of commercial kits in defining patterns and the use of solid phase methodologies to determine the presence of autoantibodies. (C) 2014 Elsevier Editora Ltda. All rights reserved. PUC Goias, Goiania, Go, Brazil Fleury Med & Saude, São Paulo, Brazil Universidade Federal de São Paulo UNIFESP, Discipline Rheumatol, São Paulo, Brazil Univ Fed Uberlandia, Hosp Clin, Serv Rheumatol, BR-38400 Uberlandia, MG, Brazil Clin Doencas Reumat Porto Alegre, Ctr Diagnost Med & Rheuma, Porto Alegre, RS, Brazil Amaral Costa Med Diagnost, Belem, PA, Brazil Univ São Paulo, Fac Med, Hosp Clin, Lab Invest Med, São Paulo, Brazil Univ São Paulo, Fac Med, Hosp Clin, Lab Cent, São Paulo, Brazil Hosp Israelita Albert Einstein, Dept Clin Pathol, São Paulo, Brazil Inst Hermes Pardini, Belo Horizonte, MG, Brazil Univ São Paulo, Fac Med, São Paulo, Brazil Univ Fed Minas Gerais, Fac Med, Belo Horizonte, MG, Brazil Univ Catolica Brasilia, Brasilia, DF, Brazil Univ Fed Goias, Fac Med, Goiania, Go, Brazil Univ Sul Santa Catarina UNISUL, Florianopolis, SC, Brazil Univ Vale do Itajai UNIVALE, Florianopolis, SC, Brazil Univ Fed Espirito Santo, Vitoria, ES, Brazil EBMSP, Salvador, BA, Brazil Grp DASA, São Paulo, Brazil Escola Super Ciencias Saude Dist Fed, Brasilia, DF, Brazil Lab Sabin, Brasilia, DF, Brazil Universidade Federal de São Paulo UNIFESP, Discipline Rheumatol, São Paulo, Brazil Web of Science