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1. Circulating Amino Acids and Risk of Peripheral Artery Disease in the PREDIMED Trial

Author

Cristina Razquin, Miguel Ruiz-Canela, Estefania Toledo, Clary B. Clish, Marta Guasch-Ferré, Jesús F. García-Gavilán, Clemens Wittenbecher, Angel Alonso-Gómez, Montse Fitó, Liming Liang, Dolores Corella, Enrique Gómez-Gracia, Ramon Estruch, Miquel Fiol, Jose M. Santos-Lozano, Luis Serra-Majem, Emilio Ros, Fernando Aros, Jordi Salas-Salvadó, Frank B. Hu, Miguel A. Martínez-González, [Razquin, Cristina] Univ Navarra, Dept Prevent Med & Publ Hlth, IdiSNA Inst Invest Sanitaria Navarra, Pamplona 31008, Spain, [Ruiz-Canela, Miguel] Univ Navarra, Dept Prevent Med & Publ Hlth, IdiSNA Inst Invest Sanitaria Navarra, Pamplona 31008, Spain, [Toledo, Estefania] Univ Navarra, Dept Prevent Med & Publ Hlth, IdiSNA Inst Invest Sanitaria Navarra, Pamplona 31008, Spain, [Martinez-Gonzalez, Miguel A.] Univ Navarra, Dept Prevent Med & Publ Hlth, IdiSNA Inst Invest Sanitaria Navarra, Pamplona 31008, Spain, [Razquin, Cristina] Inst Salud Carlos III, CIBER Fisiopatol Obes & Nutr CIBERObn, Madrid 28029, Spain, [Ruiz-Canela, Miguel] Inst Salud Carlos III, CIBER Fisiopatol Obes & Nutr CIBERObn, Madrid 28029, Spain, [Toledo, Estefania] Inst Salud Carlos III, CIBER Fisiopatol Obes & Nutr CIBERObn, Madrid 28029, Spain, [Garcia-Gavilan, Jesus F.] Inst Salud Carlos III, CIBER Fisiopatol Obes & Nutr CIBERObn, Madrid 28029, Spain, [Alonso-Gomez, Angel] Inst Salud Carlos III, CIBER Fisiopatol Obes & Nutr CIBERObn, Madrid 28029, Spain, [Fito, Montse] Inst Salud Carlos III, CIBER Fisiopatol Obes & Nutr CIBERObn, Madrid 28029, Spain, [Corella, Dolores] Inst Salud Carlos III, CIBER Fisiopatol Obes & Nutr CIBERObn, Madrid 28029, Spain, [Estruch, Ramon] Inst Salud Carlos III, CIBER Fisiopatol Obes & Nutr CIBERObn, Madrid 28029, Spain, [Fiol, Miquel] Inst Salud Carlos III, CIBER Fisiopatol Obes & Nutr CIBERObn, Madrid 28029, Spain, [Santos-Lozano, Jose M.] Inst Salud Carlos III, CIBER Fisiopatol Obes & Nutr CIBERObn, Madrid 28029, Spain, [Serra-Majem, Luis] Inst Salud Carlos III, CIBER Fisiopatol Obes & Nutr CIBERObn, Madrid 28029, Spain, [Ros, Emilio] Inst Salud Carlos III, CIBER Fisiopatol Obes & Nutr CIBERObn, Madrid 28029, Spain, [Aros, Fernando] Inst Salud Carlos III, CIBER Fisiopatol Obes & Nutr CIBERObn, Madrid 28029, Spain, [Salas-Salvado, Jordi] Inst Salud Carlos III, CIBER Fisiopatol Obes & Nutr CIBERObn, Madrid 28029, Spain, [Martinez-Gonzalez, Miguel A.] Inst Salud Carlos III, CIBER Fisiopatol Obes & Nutr CIBERObn, Madrid 28029, Spain, [Clish, Clary B.] Harvard Univ, Broad Inst MIT, Cambridge, MA 02142 USA, [Guasch-Ferre, Marta] Harvard TH Chan Sch Publ Hlth, Dept Nutr, Boston, MA 02115 USA, [Wittenbecher, Clemens] Harvard TH Chan Sch Publ Hlth, Dept Nutr, Boston, MA 02115 USA, [Hu, Frank B.] Harvard TH Chan Sch Publ Hlth, Dept Nutr, Boston, MA 02115 USA, [Martinez-Gonzalez, Miguel A.] Harvard TH Chan Sch Publ Hlth, Dept Nutr, Boston, MA 02115 USA, [Guasch-Ferre, Marta] Univ Copenhagen, Dept Publ Hlth, Sect Epidemiol, DK-1165 Copenhagen, Denmark, [Guasch-Ferre, Marta] Univ Copenhagen, Novo Nord Fdn Ctr Basic Metab Res, DK-1353 Copenhagen, Denmark, [Garcia-Gavilan, Jesus F.] Univ Rovira i Virgili, Dept Bioquim & Biotecnol, Unitat Nutr Humana, Reus 43201, Spain, [Salas-Salvado, Jordi] Univ Rovira i Virgili, Dept Bioquim & Biotecnol, Unitat Nutr Humana, Reus 43201, Spain, [Garcia-Gavilan, Jesus F.] Hosp Univ San Joan Reus, Inst Invest Sanitaria Pere Virgili IISPV, Reus 43204, Spain, [Salas-Salvado, Jordi] Hosp Univ San Joan Reus, Inst Invest Sanitaria Pere Virgili IISPV, Reus 43204, Spain, [Wittenbecher, Clemens] Chalmers Univ Technol, Dept Biol & Biol Engn, Food & Nutr Sci Div, SciLifeLab, SE-41296 Gothenburg, Sweden, [Alonso-Gomez, Angel] Univ Basque Country UPV EHU, Araba Univ Hosp, Bioaraba Hlth Res Inst, Osakidetza Basque Hlth Serv, Vitoria 01009, Spain, [Aros, Fernando] Univ Basque Country UPV EHU, Araba Univ Hosp, Bioaraba Hlth Res Inst, Osakidetza Basque Hlth Serv, Vitoria 01009, Spain, [Fito, Montse] Inst Hosp del Mar Invest Med IMIM, Unit Cardiovasc Risk & Nutr, Barcelona 08003, Spain, [Liang, Liming] Harvard TH Chan Sch Publ Hlth, Dept Epidemiol & Biostat, Boston, MA 02115 USA, [Corella, Dolores] Univ Valencia, Dept Prevent Med, Valencia 46100, Spain, [Gomez-Gracia, Enrique] Univ Malaga, Dept Prevent Med, Malaga 29071, Spain, [Estruch, Ramon] Univ Barcelona, Hosp Clin, Inst Invest Biomed August Pi Sunyer IDIBAPS, Dept Internal Med, Barcelona 08036, Spain, [Fiol, Miquel] Hosp Univ Son Espases, Plataforma Ensayos Clin, Inst Invest Sanitaria Illes Balears IdISBa, Palma De Mallorca 07120, Spain, [Santos-Lozano, Jose M.] Dist Sanitario Atenc Primaria Sevilla, Dept Family Med, Res Unit, Seville 41013, Spain, [Serra-Majem, Luis] Univ Las Palmas Gran Canaria, Res Inst Biomed & Hlth Sci IUIBS, Nutr Res Grp, Las Palmas Gran Canaria 35016, Spain, [Ros, Emilio] Univ Barcelona, Hosp Clin, Agust Pi Sunyer Biomed Res Inst IDIBAPS i, Dept Endocrinol & Nutr,Lipid Clin, Barcelona 08036, Spain, and [Hu, Frank B.] Harvard Med Sch, Brigham & Womens Hosp, Dept Med, Channing Div Network Med, Boston, MA 02115 USA

Subjects
PREDIMED, amino acids, Predictors, Organic Chemistry, General Medicine, case-cohort, peripheral artery disease, metabolomics, Catalysis, Computer Science Applications, Association, Inorganic Chemistry, Life, Health, Physical and Theoretical Chemistry, Ratio, Molecular Biology, Spectroscopy
Abstract

Effective prevention and risk prediction are important for peripheral artery disease (PAD) due to its poor prognosis and the huge disease burden it produces. Circulating amino acids (AA) and their metabolites may serve as biomarkers of PAD risk, but they have been scarcely investigated. The objective was to prospectively analyze the associations of baseline levels of plasma AA (and their pathways) with subsequent risk of PAD and the potential effect modification by a nutritional intervention with the Mediterranean diet (MedDiet). A matched case-control study was nested in the PREDIMED trial, in which participants were randomized to three arms: MedDiet with tree nut supplementation group, MedDiet with extra-virgin olive oil (EVOO) supplementation group or control group (low-fat diet). One hundred and sixty-seven PAD cases were matched with 250 controls. Plasma AA was measured with liquid chromatography/mass spectrometry at the Broad Institute. Baseline tryptophan, serine and threonine were inversely associated with PAD (ORfor 1 SD increase = 0.78 (0.61–0.99); 0.67 (0.51–0.86) and 0.75 (0.59–0.95), respectively) in a multivariable-adjusted conditional logistic regression model. The kynurenine/tryptophan ratio was directly associated with PAD (ORfor 1 SD increase = 1.50 (1.14–1.98)). The nutritional intervention with the MedDiet+nuts modified the association between threonine and PAD (p-value interaction = 0.018) compared with the control group. However, subjects allocated to the MedDiet+EVOO group were protected against PAD independently of baseline threonine. Plasma tryptophan, kynurenine/tryptophan ratio, serine and threonine might serve as early biomarkers of future PAD in subjects at a high risk of cardiovascular disease. The MedDiet supplemented with EVOO exerted a protective effect, regardless of baseline levels of threonine.

Published
2022

2. A Morphological Method for Ammonia Detection in Liver

Author

Imanol Zubiete-Franco, Paula Iruzubieta, Virginia Gutiérrez-de-Juan, María Teresa Arias-Loste, Sergio López de Davalillo, M.L. Martínez-Chantar, Pablo Fernández-Tussy, Fernando Lopitz-Otsoa, José M. Mato, M. Isabel Lucena, David Fernández-Ramos, Raúl J. Andrade, Javier Crespo, Javier de las Heras, Erica Villa, Jorge Simón, Lucía Barbier-Torres, Marta Varela-Rey, Teresa C. Delgado, Shelly C. Lu, Avila, Matias A, [Gutierrez-de-Juan, Virginia] Ctr Invest Biomed Red Enfermedades Hepat & Digest, CIC bioGUNE Ctr Cooperat Res Biosci, Derio, Bizkaia, Spain, [Lopez de Davalillo, Sergio] Ctr Invest Biomed Red Enfermedades Hepat & Digest, CIC bioGUNE Ctr Cooperat Res Biosci, Derio, Bizkaia, Spain, [Fernandez-Ramos, David] Ctr Invest Biomed Red Enfermedades Hepat & Digest, CIC bioGUNE Ctr Cooperat Res Biosci, Derio, Bizkaia, Spain, [Barbier-Torres, Lucia] Ctr Invest Biomed Red Enfermedades Hepat & Digest, CIC bioGUNE Ctr Cooperat Res Biosci, Derio, Bizkaia, Spain, [Zubiete-Franco, Imanol] Ctr Invest Biomed Red Enfermedades Hepat & Digest, CIC bioGUNE Ctr Cooperat Res Biosci, Derio, Bizkaia, Spain, [Fernandez-Tussy, Pablo] Ctr Invest Biomed Red Enfermedades Hepat & Digest, CIC bioGUNE Ctr Cooperat Res Biosci, Derio, Bizkaia, Spain, [Simon, Jorge] Ctr Invest Biomed Red Enfermedades Hepat & Digest, CIC bioGUNE Ctr Cooperat Res Biosci, Derio, Bizkaia, Spain, [Lopitz-Otsoa, Fernando] Ctr Invest Biomed Red Enfermedades Hepat & Digest, CIC bioGUNE Ctr Cooperat Res Biosci, Derio, Bizkaia, Spain, [Varela-Rey, Marta] Ctr Invest Biomed Red Enfermedades Hepat & Digest, CIC bioGUNE Ctr Cooperat Res Biosci, Derio, Bizkaia, Spain, [Mato, Jose M.] Ctr Invest Biomed Red Enfermedades Hepat & Digest, CIC bioGUNE Ctr Cooperat Res Biosci, Derio, Bizkaia, Spain, [Cardoso Delgado, Teresa] Ctr Invest Biomed Red Enfermedades Hepat & Digest, CIC bioGUNE Ctr Cooperat Res Biosci, Derio, Bizkaia, Spain, [Martinez-Chantar, Maria-Luz] Ctr Invest Biomed Red Enfermedades Hepat & Digest, CIC bioGUNE Ctr Cooperat Res Biosci, Derio, Bizkaia, Spain, [de las Heras, Javier] Univ Basque Country, UPV EHU, BioCruces Hlth Res Inst, Div Pediat Metab,Univ Hosp Cruces, Baracaldo, Bizkaia, Spain, [Crespo, Javier] Univ Basque Country, UPV EHU, BioCruces Hlth Res Inst, Div Pediat Metab,Univ Hosp Cruces, Baracaldo, Bizkaia, Spain, [Iruzubieta, Paula] Ctr Invest Biomed Red Enfermedades Hepat & Digest, Dept Gastroenterol & Hepatol, Marques de Valdecilla Univ Hosp, Santander, Spain, [Teresa Arias-Loste, Maria] Ctr Invest Biomed Red Enfermedades Hepat & Digest, Dept Gastroenterol & Hepatol, Marques de Valdecilla Univ Hosp, Santander, Spain, [Crespo, Javier] Ctr Invest Biomed Red Enfermedades Hepat & Digest, Dept Gastroenterol & Hepatol, Marques de Valdecilla Univ Hosp, Santander, Spain, [Iruzubieta, Paula] Res Inst Marques de Valdecilla IDIVAL, Infect Immun & Digest Pathol Grp, Santander, Spain, [Teresa Arias-Loste, Maria] Res Inst Marques de Valdecilla IDIVAL, Infect Immun & Digest Pathol Grp, Santander, Spain, [Villa, Erica] Azienda Osped Univ, Dept Gastroenterol, Modena, Italy, [Villa, Erica] Univ Modena & Reggio Emilia, Modena, Italy, [Andrade, Raul] Univ Malaga, Ctr Invest Biomed Red Enfermedades Hepat & Digest, Hosp Univ Virgen de la Victoria,Inst Invest Biome, Unidad Gest Clin Aparato Digest,Serv Farmacol Cli, Malaga, Spain, [Isabel Lucena, M.] Univ Malaga, Ctr Invest Biomed Red Enfermedades Hepat & Digest, Hosp Univ Virgen de la Victoria,Inst Invest Biome, Unidad Gest Clin Aparato Digest,Serv Farmacol Cli, Malaga, Spain, [Lu, Shelly C.] Cedars Sinai Med Ctr, Div Digest & Liver Dis, Los Angeles, CA 90048 USA, NIH (USDepartment of Health and Human services), Gobierno Vasco-Departamento de Salud, MINECO, Instituto de Salud Carlos III, integrado en el Plan Estatal de Investigacion Cientifica y Tecnica y Innovacion 2013-2016 cofinanciado con Fondos FEDER, EITB, Asociacion Espanola contra el Cancer, National Center for Complementary & Integrative Health, and NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES

Subjects
0301 basic medicine, Male, Cirrhosis, medicine.medical_treatment, Biopsy, lcsh:Medicine, Liver transplantation, Chronic liver disease, Gastroenterology, Oral and gastrointestinal, Liver disease, Mice, 0302 clinical medicine, Non-alcoholic Fatty Liver Disease, Medicine and Health Sciences, hepatic-encephalopathy, Medicine, 2.1 Biological and endogenous factors, Disease, Aetiology, lcsh:Science, Child, Hepatic encephalopathy, risk, Liver injury, Multidisciplinary, Mercury Compounds, Liver Disease, Liver Diseases, Fatty liver, Hyperammonemia, Animal Models, Middle Aged, 3. Good health, failure, Chemistry, Liver, Experimental Organism Systems, Child, Preschool, Physical Sciences, 030211 gastroenterology & hepatology, Research Article, Adult, medicine.medical_specialty, hypertension, Adolescent, hyperammonemia, complications, General Science & Technology, Chronic Liver Disease and Cirrhosis, Cytological Techniques, Mouse Models, Surgical and Invasive Medical Procedures, Gastroenterology and Hepatology, Research and Analysis Methods, 03 medical and health sciences, Young Adult, Digestive System Procedures, Rare Diseases, Model Organisms, Ammonia, Internal medicine, arterial ammonia, Animals, Humans, Aged, Iodides, Biochemistry, Genetics and Molecular Biology (all), Agricultural and Biological Sciences (all), Preschool, Nutrition, Transplantation, business.industry, Prevention, cirrhosis, lcsh:R, Chemical Compounds, Biology and Life Sciences, Organ Transplantation, medicine.disease, Liver Transplantation, Diet, Fatty Liver, 030104 developmental biology, cells, lcsh:Q, business, Digestive Diseases
Abstract

Hyperammonemia is a metabolic condition characterized by elevated levels of ammonia and a common event in acute liver injury/ failure and chronic liver disease. Even though hepatic ammonia levels are potential predictive factors of patient outcome, easy and inexpensive methods aiming at the detection of liver ammonia accumulation in the clinical setting remain unavailable. Thus, herein we have developed a morphological method, based on the utilization of Nessler A s reagent, to accurately and precisely detect the accumulation of ammonia in biological tissue. We have validated our method against a commercially available kit in mouse tissue samples and, by using this modified method, we have confirmed the hepatic accumulation of ammonia in clinical and animal models of acute and chronic advanced liver injury as well as in the progression of fatty liver disease. Overall, we propose a morphological method for ammonia detection in liver that correlates well with the degree of liver disease severity and therefore can be potentially used to predict patient outcome. This work was supported by grants from NIH (USDepartment of Health and Human services)-R01AT001576 (to S.C.L., J.M.M and M.L.M-C.), Gobierno Vasco-Departamento de Salud 2013111114 (to M.L.M.-C), MINECO: SAF20145 4658-R and SAF2014-52097-R integrado en el Plan Estatal de Investigacion Cientifica y Tecnica y Innovacio 2013-2016 cofinanciado con Fondos FEDER to M.L.M.-C and J.M.M. respectively, Instituto de Salud Carlos III:PIE14/00031, integrado en el Plan Estatal de Investigacion Cientifica y Tecnica y Innovacion 2013-2016 cofinanciado con Fondos FEDER (toM.L.M.-C and J.M.M),EITB. BIO15/CA/014, Asociacion Espanola contra el Cancer (T.C.D, P.F-Tand M.L.M-C). Ciberehd_ISCIII_MINECO is funded by the Instituto de Salud Carlos III.Thefunders had no role in study design,datacollection and analysis,decision to publish,or preparation of the manuscript.

Published
2017

3. Poor phenotype-genotype association in a large series of patients with Type III Bartter syndrome

Author

Blanco, Francisco J., García Castaño, Alejandro, Pérez de Nanclares, Gustavo, Madariaga Domínguez, Leire, Aguirre, Mireia, Madrid, Álvaro, Chocrón, Sara, Nadal, Inmaculada, Navarro, Mercedes, Lucas, Elena, Fijo, Julia, Espino, Mar, Espitaletta, Zilac, García Nieto, Víctor, Barajas de Frutos, David, Loza, Reyner, Pintos, Guillem, Castaño González, Luis Antonio, RenalTube Group, Ariceta, Gema, [Garcia Castano, Alejandro] Cruces Univ Hosp, Ciberer, BioCruces Hlth Res Inst, Bizkaia, Spain, [Perez de Nanclares, Gustavo] Cruces Univ Hosp, Ciberer, BioCruces Hlth Res Inst, Bizkaia, Spain, [Castano, Luis] Cruces Univ Hosp, Ciberer, BioCruces Hlth Res Inst, Bizkaia, Spain, [Madariaga, Leire] Cruces Univ Hosp, Pediat Nephrol, Bizkaia, Spain, [Aguirre, Mireia] Cruces Univ Hosp, Pediat Nephrol, Bizkaia, Spain, [Madariaga, Leire] Univ Basque Country, UPV EHU, Dept Pediat, Sch Med & Odontol, Bizkaia, Spain, [Castano, Luis] Univ Basque Country, UPV EHU, Dept Pediat, Sch Med & Odontol, Bizkaia, Spain, [Madrid, Alvaro] Univ Autonoma Barcelona, Vall dHebron Univ Hosp, Pediat Nephrol, Barcelona, Spain, [Chocron, Sara] Univ Autonoma Barcelona, Vall dHebron Univ Hosp, Pediat Nephrol, Barcelona, Spain, [Ariceta, Gema] Univ Autonoma Barcelona, Vall dHebron Univ Hosp, Pediat Nephrol, Barcelona, Spain, [Nadal, Inmaculada] Virgen del Camino Hosp, Pediat Nephrol, Pamplona, Spain, [Navarro, Mercedes] La Paz Univ Hosp, Pediat Nephrol, Madrid, Spain, [Lucas, Elena] Manises Hosp, Pediat, Valencia, Spain, [Fijo, Julia] Virgen del Rocio Hosp, Pediat Nephrol, Seville, Spain, [Espino, Mar] Fdn Alcorcon Univ Hosp, Pediat Nephrol, Madrid, Spain, [Espitaletta, Zilac] San Ignacio Univ Hosp, Bogota, Colombia, [Garcia Nieto, Victor] Nuestra Senora de Candelaria Univ Hosp, Pediat Nephrol, Tenerife, Canarias, Spain, [Barajas de Frutos, David] Virgen de las Nieves Hosp, Pediat Nephrol, Granada, Spain, [Loza, Reyner] Cayetano Heredia Univ, Cayetano Heredia Hosp, Nephrol Unit, Lima, Peru, [Pintos, Guillem] Germans Trias & Pujol Univ Hosp, Badalona, Spain, [Castano, Luis] Inst Salud Carlos III, Ctr Invest Biomed Red Diabet & Enfermedades Metab, Madrid, Spain, [RenalTube Grp] Asturias Cent Univ Hosp, Pediat Nephrol, Oviedo, Asturias, Spain, FIS of the Institute de Salud Carlos III, Madrid, Spain, Department of Health of the Basque Government, Department of Education of the Basque Government, Institut Català de la Salut, [García Castaño A, Pérez de Nanclares G] BioCruces Health Research Institute, Ciberer, Cruces University Hospital, Bizkaia, Spain. [Madariaga L] Pediatric Nephrology, Cruces University Hospital, Bizkaia, Spain. Department of Pediatrics, School of Medicine and Odontology, University of Basque Country UPV/EHU, Bizkaia, Spain. [Aguirre M] Pediatric Nephrology, Cruces University Hospital, Bizkaia, Spain. [Madrid Á, Chocrón S, Ariceta G] Servei de Nefrologia Pediàtrica, Vall d'Hebron Hospital Universitari, Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain, Vall d'Hebron Barcelona Hospital Campus, RenalTube Group, [García Castaño,A, Pérez de Nanclares,G, Castaño,L] BioCruces Health Research Institute, Ciberer, Cruces University Hospital, Bizkaia, Spain. [Madariaga,L, Aguirre,M] Pediatric Nephrology, Cruces University Hospital, Bizkaia, Spain. [Madariaga,L, Castaño,L] Department of Pediatrics, School of Medicine and Odontology, University of Basque Country UPV/EHU, Bizkaia, Spain. [Madrid,A, Chocrón,S, and Ariceta,G] Pediatric Nephrology, Vall d’Hebron University Hospital, Universitat Autonoma, Barcelona, Spain. [Nadal,I] Pediatric Nephrology, Virgen del Camino Hospital, Pamplona, Spain. [Navarro,M] Pediatric Nephrology, La Paz University Hospital, Madrid, Spain. [Lucas,E] Pediatrics, Manises Hospital, Valencia, Spain. [Fijo,J] Pediatric Nephrology, Virgen del Rocío Hospital, Sevilla, Spain. [Espino,M] Pediatric Nephrology, Fundación Alcorcón University Hospital, Madrid, Spain. [Espitaletta,Z] San Ignacio University Hospital, Bogotá, Colombia. [García Nieto,V] Pediatric Nephrology, Nuestra Señora de Candelaria University Hospital, Tenerife, Canarias, Spain. [Barajas de Frutos,D] Pediatric Nephrology, Virgen de las Nieves Hospital, Granada, Spain. [Loza,R] Nephrology Unit, Cayetano Heredia University, Cayetano Heredia Hospital, Lima, Peru. [Pintos,G] Germans Trias i Pujol University Hospital, Badalona, Spain. [Castaño,L] BioCruces Health Research Institute, Ciberer, Cruces University Hospital, Bizkaia, Spain, Department of Pediatrics, School of Medicine and Odontology, University of Basque Country UPV/EHU, Bizkaia, Spain, Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Instituto de Salud Carlos III, Madrid, Spain.

Subjects
0301 basic medicine, Male, Pediatrics, Spanish People, chloride channel gene, Physiology, Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Carrier Proteins::Membrane Transport Proteins::Ion Channels::Chloride Channels [Medical Subject Headings], 030232 urology & nephrology, Gene Identification and Analysis, lcsh:Medicine, Endocrine System Diseases::Adrenal Gland Diseases::Adrenocortical Hyperfunction::Hyperaldosteronism::Bartter Syndrome [DISEASES], Urine, Phenomena and Processes::Genetic Phenomena::Genetic Structures::Genome::Genome Components::Genes::Gene Components::Exons [Medical Subject Headings], Diseases::Nutritional and Metabolic Diseases::Metabolic Diseases::Water-Electrolyte Imbalance::Dehydration [Medical Subject Headings], Alcalosis, Deshidratación, Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings], 0302 clinical medicine, Diseases::Nutritional and Metabolic Diseases::Metabolic Diseases::Acid-Base Imbalance::Alkalosis [Medical Subject Headings], clcnkb, Reacción en cadena de la polimerasa, Genotype, Medicine and Health Sciences, Medicine, Ethnicities, lcsh:Science, Multidisciplinary, Diseases::Pathological Conditions, Signs and Symptoms::Pathologic Processes::Polydipsia [Medical Subject Headings], Otros calificadores::Otros calificadores::/genética [Otros calificadores], Eliminación de secuencia, gitelman, Large series, Ronyons - Malalties, Polidipsia, Humanos, Molecular analysis, Body Fluids, Deletion Mutation, Phenotype, Hipopotasemia, Child, Preschool, Phenotype genotype, Diseases::Nutritional and Metabolic Diseases::Metabolic Diseases::Water-Electrolyte Imbalance::Hypokalemia [Medical Subject Headings], Science & Technology - Other Topics, Female, Phenomena and Processes::Genetic Phenomena::Genotype [Medical Subject Headings], Malalties congènites, Phenomena and Processes::Genetic Phenomena::Genetic Structures::Genome::Genome Components::DNA, Intergenic::Introns [Medical Subject Headings], Anatomy, enfermedades del sistema endocrino::enfermedades de las glándulas suprarrenales::hiperfunción corticosuprarrenal::hiperaldosteronismo::síndrome de Bartter [ENFERMEDADES], Alelos, Research Article, medicine.medical_specialty, salt-losing tubulopathies, Diseases::Nutritional and Metabolic Diseases::Metabolic Diseases::Calcium Metabolism Disorders::Calcinosis::Nephrocalcinosis [Medical Subject Headings], Síndrome de bartter, Excretion, Diseases::Endocrine System Diseases::Adrenal Gland Diseases::Adrenocortical Hyperfunction::Hyperaldosteronism::Bartter Syndrome [Medical Subject Headings], Health Care::Health Services Administration::Organization and Administration::Professional Practice::Referral and Consultation [Medical Subject Headings], Diseases::Pathological Conditions, Signs and Symptoms::Signs and Symptoms::Urological Manifestations::Hypercalciuria [Medical Subject Headings], Bartter syndrome, Exones, 03 medical and health sciences, nephrocalcinosis, Genetics, Other subheadings::Other subheadings::/genetics [Other subheadings], Intrones, Point Mutation, Humans, Mutation detection, molecular analysis, Nefrocalcinosis, Mutation Detection, business.industry, lcsh:R, Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Nucleic Acid Amplification Techniques::Polymerase Chain Reaction [Medical Subject Headings], Biology and Life Sciences, Bartter Syndrome, Infant, Human Genetics, medicine.disease, mutations, purl.org/pe-repo/ocde/ford#3.01.02 [https], 030104 developmental biology, Deletion mutation, Mutation, People and Places, Hipercalciuria, Phenomena and Processes::Genetic Phenomena::Genetic Processes::Mutagenesis::Sequence Deletion [Medical Subject Headings], lcsh:Q, Population Groupings, Remisión y consulta, business, Physiological Processes, Genotipo, Canales de cloruro
Abstract

Excreció; Genètica humana; Mutació Excreción; Genética humana; Mutación Excretion; Human genetics; Mutation Introduction Type III Bartter syndrome (BS) is an autosomal recessive renal tubule disorder caused by loss-of-function mutations in the CLCNKB gene, which encodes the chloride channel protein ClC-Kb. In this study, we carried out a complete clinical and genetic characterization in a cohort of 30 patients, one of the largest series described. By comparing with other published populations, and considering that 80% of our patients presented the p.Ala204Thr Spanish founder mutation presumably associated with a common phenotype, we aimed to test the hypothesis that allelic differences could explain the wide phenotypic variability observed in patients with type III BS. Methods Clinical data were retrieved from the referral centers. The exon regions and flanking intronic sequences of the CLCNKB gene were screened for mutations by polymerase chain reaction (PCR) followed by direct Sanger sequencing. Presence of gross deletions or duplications in the region was checked for by MLPA and QMPSF analyses. Results Polyuria, polydipsia and dehydration were the main common symptoms. Metabolic alkalosis and hypokalemia of renal origin were detected in all patients at diagnosis. Calciuria levels were variable: hypercalciuria was detected in 31% of patients, while 23% had hypocalciuria. Nephrocalcinosis was diagnosed in 20% of the cohort. Two novel CLCNKB mutations were identified: a small homozygous deletion (c.753delG) in one patient and a small deletion (c.1026delC) in another. The latter was present in compound heterozygosis with the already previously described p.Glu442Gly mutation. No phenotypic association was obtained regarding the genotype. Conclusion A poor correlation was found between a specific type of mutation in the CLCNKB gene and type III BS phenotype. Importantly, two CLCNKB mutations not previously described were found in our cohort This study was supported by two grants (PI09/90888 and PI11/01412) from the FIS of the Instituto de Salud Carlos III, Madrid, Spain, the Department of Health of the Basque Government (2014111064), and the Department of Education of the Basque Government (IT795-13).

Published
2017

4. Physicochemical and in vitro cytotoxic properties of chitosan from mushroom species (Boletus bovinus and Laccaria laccata)

Author

Rita Saule, Vykintas Baublys, Alona Oberemko, Gintautas Saulis, Jalel Labidi, Murat Kaya, Asier M. Salaberria, [Oberemko, Alona -- Saule, Rita -- Saulis, Gintautas -- Baublys, Vykintas] Vytautas Magnus Univ, Dept Biol, Vileikos 8, LT-44248 Kaunas, Lithuania -- [Salaberria, Asier M. -- Labidi, Jalel] Univ Basque Country UPV EHU, Dept Chem & Environm Engn, Biorefinery Proc Res Grp, Plaza Europa 1, Donostia San Sebastian 20018, Spain -- [Kaya, Murat] Aksaray Univ, Dept Biotechnol & Mol Biol, Fac Sci & Letters, TR-68100 Aksaray, Turkey, Labidi, Jalel -- 0000-0002-8382-9492, and Sabire Yazıcı Fen Edebiyat Fakültesi

Subjects
Laccaria laccata, Thermogravimetric analysis, Polymers and Plastics, Deacetylation, Infrared spectroscopy, Boletus, Antineoplastic Agents, Apoptosis, CHO Cells, macromolecular substances, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Laccaria, Mushroom, Chitosan, Mice, Necrosis, chemistry.chemical_compound, Cricetulus, Cell Line, Tumor, Materials Chemistry, Animals, Cancer, biology, Chemistry, Organic Chemistry, technology, industry, and agriculture, Nuclear magnetic resonance spectroscopy, Carbon-13 NMR, equipment and supplies, 021001 nanoscience & nanotechnology, biology.organism_classification, 0104 chemical sciences, Molecular Weight, Low-Molecular weight, carbohydrates (lipids), 0210 nano-technology, Nuclear chemistry
Abstract

Kaya, Murat ( Aksaray, Yazar ), Chitosan samples from two mushroom species (Boletus bovinus, Laccaria laccata) were obtained and characterized by viscosimetry, attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), elemental analyses (EA), nuclear magnetic resonance spectroscopy (13C NMR), X-ray diffraction (XRD) and thermogravimetric (TGA) analyses. Properties of the fungal chitosan samples were compared to commercial low-molecular weight chitosan, crustacean chitosan (Cervimunida johni) and chitosan obtained from an insect (Hilobius abietis). Additionally, the cytotoxic properties of chitosan in vitro on cancerous hepatoma and non-cancerous ovary cells cultivated on films with different chitosan concentrations was evaluated. As a conclusion, this study clearly revealed that low-molecular weight chitosan films and solutions with high degree of deacetylation can act cytotoxically on both tumor MH-22A and normal CHO cells in vitro. Consequently, this work may be useful for further investigations of natural anticancer products in medical areas.

Published
2019

5. Production of novel chia-mucilage nanocomposite films with starch nanocrystals; An inclusive biological and physicochemical perspective

Author

Sami Boufi, Sedef Ilk, Jalel Labidi, Khalid Mahmood Khawar, Muhammad Mujtaba, Behlul Koc, Demet Cansaran-Duman, Murat Kaya, Asier M. Salaberria, Yavuz Selim Cakmak, Lalehan Akyuz, [Mujtaba, Muhammad -- Cansaran-Duman, Demet] Ankara Univ, Inst Biotechnol, TR-06110 Ankara, Turkey -- [Koc, Behlul -- Cakmak, Yavuz Selim -- Kaya, Murat] Aksaray Univ, Fac Sci & Letters, Dept Biotechnol & Mol Biol, TR-68100 Aksaray, Turkey -- [Martinez Salaberria, Asier -- Labidi, Jalel] Univ Basque Country UPV EHU, Dept Chem & Environm Engn, Biorefinery Proc Res Grp, Plaza Europa 1, Donostia San Sebastian 20018, Spain -- [Ilk, Sedef] Nigde Omer Halisdemir Univ, Fac Med, Dept Immunol, Nigde, Turkey -- [Akyuz, Lalehan] Aksaray Univ, Tech Vocat Sch, Dept Chem Technol, TR-68100 Aksaray, Turkey -- [Boufi, Sami] Univ Sfax, Fac Sci, LMSE, BP 802, Sfax 3018, Tunisia -- [Khawar, Khalid Mahmood] Ankara Univ, Dept Field Crops, TR-06110 Ankara, Turkey, and Sabire Yazıcı Fen Edebiyat Fakültesi

Subjects
Materials science, Chemical Phenomena, Starch, Composite number, Antineoplastic Agents, 02 engineering and technology, Biochemistry, Antioxidants, Nanocomposites, Contact angle, 03 medical and health sciences, chemistry.chemical_compound, Plant Mucilage, SDG 3 - Good Health and Well-being, Structural Biology, Ultimate tensile strength, Chlorocebus aethiops, Animals, Humans, Salvia, Molecular Biology, Vero Cells, Mucilage film, 030304 developmental biology, Cell Proliferation, 0303 health sciences, Nanocomposite, Viscosity, Temperature, Water, General Medicine, Hep G2 Cells, 021001 nanoscience & nanotechnology, Food coating, Anti-Bacterial Agents, chemistry, Mucilage, Nanocrystal, Chemical engineering, Solubility, Nanoparticles, Antimicrobial, Antioxidant, 0210 nano-technology
Abstract

WOS: 000472685700071, PubMed: 31022490, In the current study, chia mucilage composite films with starch nanocrystals (3% and 6%) were produced. The films were analyzed physicochemically (FT-IR, AFM, TGA, DSC), mechanically (Tensile strength and contact angle) and biologically (antimicrobial, antioxidant and cytotoxicity) properties. The incorporation of starch nanocrystals was confirmed through FT-IR spectra showing broad OH peak and C=O stretching and shift in N-H bending vibrations to the lower wave number. Starch nanocrystals enhanced (control 287.23 degrees C, film with 3% SNC 286.91 degrees C and film with 6% mucilage 289.41 degrees C) the thermal properties of the composite films. The Young Modulus of the film showed an increase after the incorporation of starch nanocrystals due to the strong interaction between mucilage and nanocrystals. On the other hand, the overall hydrophobicity of mucilage composite film decreased due to the hydrophilic nature of cornstarch nanocrystals. mu assay for cell proliferation revealed significant inhibition of cancer cell (HepG2) lines and exhibits a very low inhibition of epithelial cell line (Vero). Starch nanocrystals enhanced the antibacterial and antioxidant (threefold increase compare to control) properties of mucilage composite films. Mucilage-SNC composite films could be a good therapeutic gain for control and directed drug delivery, food packaging, food coating. (C) 2019 Elsevier B.V. All rights reserved.

Published
2019

6. Hydrothermal synthesis of bacterial cellulose–copper oxide nanocomposites and evaluation of their antimicrobial activity

Author

Sidney José Lima Ribeiro, Robson Rosa da Silva, Agnieszka Tercjak, Junkal Gutierrez, Inês M. Araújo, Maria Leticia Vega, José Ribeiro dos Santos Júnior, Francisco Honeidy Carvalho Azevedo, Girlene S. Figuêredo, Wilton R. Lustri, Guilherme Pacheco, Hernane da Silva Barud, Univ Fed Piaui, Universidade Estadual Paulista (Unesp), Centro Univ Araraquara, Univ Basque Country UPV EHU, Univ Luterana Brasil, and Universidade de São Paulo (USP)

Subjects
Staphylococcus aureus, Copper oxide, Hot Temperature, Time Factors, Materials science, Polymers and Plastics, Metal Nanoparticles, chemistry.chemical_element, Nanoparticle, Nanotechnology, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Hydrothermal circulation, Nanocomposites, Bacterial cellulose, Crystallinity, chemistry.chemical_compound, Anti-Infective Agents, Escherichia coli, Materials Chemistry, Hydrothermal synthesis, Cellulose, Nanocomposite, Organic Chemistry, Salmonella enterica, Water, 021001 nanoscience & nanotechnology, Copper, 0104 chemical sciences, Gluconacetobacter, chemistry, Chemical engineering, Thermogravimetry, Copper nanoparticles, 0210 nano-technology
Abstract

Made available in DSpace on 2018-11-26T17:42:24Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-01-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) In this work, for the first time bacterial cellulose (BC) hydrogel membranes were used for the fabrication of antimicrobial cellulosic nanocomposites by hydrothermal deposition of Cu derivative nanoparticles (i.e. Cu(0) and CuxOy species). BC-Cu nanocomposites were characterized by FTIR, SEM, AFM, XRD and TGA, to study the effect of hydrothermal processing time on the final physicochemical properties of final products. XRD result show that depending on heating time (3-48 h), different CuxOy phases were achieved. SEM and AFM analyses unveil the presence of the Cu(0) and copper CuxOy nanoparticles over BC fibrils while the surface of 3D network became more compact and smother for longer heating times. Furthermore, the increase of heating time placed deleterious effect on the structure of BC network leading to decrease of BC crystallinity as well as of the on-set degradation temperature. Notwithstanding, BC-Cu nanocomposites showed excellent antimicrobial activity against E. coli, S. aureus and Salmonella bacteria suggesting potential applications as bactericidal films. Univ Fed Piaui, Dept Quim, Campus Minist Petronio Portela, BR-64049550 Teresina, PI, Brazil Univ Estadual Paulista, Inst Quim Araraquara, Dept Quim Geral & Inorgan, Rua Prof Francisco Degni,55, BR-14800060 Araraquara, SP, Brazil Centro Univ Araraquara, Lab Biopolimeros & Biomat BIOPOLMAT, Rua Carlos Gomes,1217, BR-14801320 Araraquara, SP, Brazil Univ Basque Country UPV EHU, Dpto Ingn Quim & Medio Ambiente, Escuela Politecn Donostia San Sebastian, Pza Europa 1, Donostia San Sebastian 20018, Spain Univ Luterana Brasil, Programa Posgrad Genet & Toxicol Aplicada, Ave Farroupilha,8001,Predio 01, BR-92450900 Canoas, RS, Brazil Univ Sao Paulo, Inst Fis Sao Carlos, BR-13560970 Sao Carlos, SP, Brazil Univ Estadual Paulista, Inst Quim Araraquara, Dept Quim Geral & Inorgan, Rua Prof Francisco Degni,55, BR-14800060 Araraquara, SP, Brazil FAPESP: 2015/ 09833-0 FAPESP: 2016/06612-6

Published
2018

7. Biological, mechanical, optical and physicochemical properties of natural chitin films obtained from the dorsal pronotum and the wing of cockroach

Author

Ignas Sabeckis, Vaida Tubelytė, Vykintas Baublys, Murat Kaya, Demet Erdönmez, Deimante Noreikaite, Jalel Labidi, Asier M. Salaberria, Idris Sargin, Sabire Yazıcı Fen Edebiyat Fakültesi, Sargin, Idris -- 0000-0003-3785-9575, Labidi, Jalel -- 0000-0002-8382-9492, Kaya, Murat -- 0000-0001-6954-2703, and [Kaya, Murat -- Sargin, Idris -- Sabeckis, Ignas -- Noreikaite, Deimante] Aksaray Univ, Dept Biotechnol & Mol Biol, TR-68100 Aksaray, Turkey -- [Sabeckis, Ignas -- Noreikaite, Deimante -- Baublys, Vykintas -- Tubelyte, Vaida] Vytautas Magnus Univ, Dept Biol, LT-44404 Kaunas, Lithuania -- [Erdonmez, Demet] Aksaray Univ, Fac Sci & Letters, Dept Biol, TR-68100 Aksaray, Turkey -- [Salaberria, Asier M. -- Labidi, Jalel] Univ Basque Country UPV EHU, Dept Chem & Environm Eng, Biorefinery Proc Res Grp, Plaza Europa 1, Donostia San Sebastian 20018, Spain

Subjects
Blaberus giganteus, Polymers and Plastics, media_common.quotation_subject, Hydrophobicity, Chitin, Cockroaches, macromolecular substances, 02 engineering and technology, Insect, 010402 general chemistry, 01 natural sciences, Microbiology, chemistry.chemical_compound, biology.animal, Materials Chemistry, Animals, Wings, Animal, Thermal stability, Blaberus Giganteus, media_common, Cockroach, biology, fungi, Organic Chemistry, Biofilm, 021001 nanoscience & nanotechnology, biology.organism_classification, 0104 chemical sciences, carbohydrates (lipids), chemistry, Nanofiber, Biophysics, Antimicrobial, 0210 nano-technology, Hydrophobic and Hydrophilic Interactions, Bacteria, Biodegradable Film
Abstract

WOS: 000396955500019, PubMed: 28267493, In previous studies, chitin-based films were produced from chitin nanofibers in dust form and fully characterized. However, chitin films naturally present.in many organisms have not been isolated and characterized. Herein, structurally intact chitin films were successfully extracted from the dorsal pronoturn and the wing of cockroach. Despite using the same extraction procedure, important differences were observed. Especially, hydrophobicity, transparency, antifungal and antibacterial biofilm activities of wing chitin film were recorded notably higher than those of chitin film from the dorsal pronotum. However, better mechanical properties were observed for chitin film from the dorsal pronotum. Notably, among the tested bacteria, two common pathogens could not form biofilms on the surface of the films. This study clearly demonstrated natural chitin films obtained from an insect can provide a new perspective to chitin-based applications where chitin films with high thermal stability, transparency, resistance to bacterial biofilm formation and antifungal activity are needed. (C) 2017 Elsevier Ltd. All rights reserved.

Published
2017

8. Leisure time physical activity is associated with improved HDL functionality in high cardiovascular risk individuals: a cohort study

Author

Ángel M. Alonso-Gómez, Miquel Fiol, Jordi Salas-Salvadó, Montserrat Fitó, Enrique Gómez-Gracia, Olga Castañer, José Lapetra, Helmut Schröder, Camille Lassale, María Trinidad Soria-Florido, Dolores Corella, Álvaro Hernáez, Ramon Estruch, Emilio Ros, Miguel Ángel Martínez-González, Lluis Serra-Majem, Xavier Pintó, [Hernaez, Alvaro] Hosp del Mar Med Res Inst IMIM, Barcelona, Spain, [Soria-Florido, Maria Trinidad] Hosp del Mar Med Res Inst IMIM, Barcelona, Spain, [Castaner, Olga] Hosp del Mar Med Res Inst IMIM, Barcelona, Spain, [Schroder, Helmut] Hosp del Mar Med Res Inst IMIM, Barcelona, Spain, [Fito, Montserrat] Hosp del Mar Med Res Inst IMIM, Barcelona, Spain, [Lassale, Camille] Hosp del Mar Med Res Inst IMIM, Barcelona, Spain, [Hernaez, Alvaro] August Pi i Sunyer Biomed Res Inst IDIBAPS, Barcelona, Spain, [Estruch, Ramon] August Pi i Sunyer Biomed Res Inst IDIBAPS, Barcelona, Spain, [Hernaez, Alvaro] Inst Salud Carlos III, CIBER Pathophysiol Obes & Nutr CIBEROBN, Madrid, Spain, [Castaner, Olga] Inst Salud Carlos III, CIBER Pathophysiol Obes & Nutr CIBEROBN, Madrid, Spain, [Pinto, Xavier] Inst Salud Carlos III, CIBER Pathophysiol Obes & Nutr CIBEROBN, Madrid, Spain, [Estruch, Ramon] Inst Salud Carlos III, CIBER Pathophysiol Obes & Nutr CIBEROBN, Madrid, Spain, [Salas-Salvado, Jordi] Inst Salud Carlos III, CIBER Pathophysiol Obes & Nutr CIBEROBN, Madrid, Spain, [Corella, Dolores] Inst Salud Carlos III, CIBER Pathophysiol Obes & Nutr CIBEROBN, Madrid, Spain, [Alonso-Gomez, Angel] Inst Salud Carlos III, CIBER Pathophysiol Obes & Nutr CIBEROBN, Madrid, Spain, [Angel Martinez-Gonzalez, Miguel] Inst Salud Carlos III, CIBER Pathophysiol Obes & Nutr CIBEROBN, Madrid, Spain, [Ros, Emilio] Inst Salud Carlos III, CIBER Pathophysiol Obes & Nutr CIBEROBN, Madrid, Spain, [Serra-Majem, Lluis] Inst Salud Carlos III, CIBER Pathophysiol Obes & Nutr CIBEROBN, Madrid, Spain, [Fiol, Miguel] Inst Salud Carlos III, CIBER Pathophysiol Obes & Nutr CIBEROBN, Madrid, Spain, [Lapetra, Jose] Inst Salud Carlos III, CIBER Pathophysiol Obes & Nutr CIBEROBN, Madrid, Spain, [Gomez-Gracia, Enrique] Inst Salud Carlos III, CIBER Pathophysiol Obes & Nutr CIBEROBN, Madrid, Spain, [Fito, Montserrat] Inst Salud Carlos III, CIBER Pathophysiol Obes & Nutr CIBEROBN, Madrid, Spain, [Lassale, Camille] Inst Salud Carlos III, CIBER Pathophysiol Obes & Nutr CIBEROBN, Madrid, Spain, [Pinto, Xavier] Hosp Univ Bellvitge, Lhospitalet De Llobregat, Spain, [Estruch, Ramon] Hosp Clin Barcelona, Barcelona, Spain, [Ros, Emilio] Hosp Clin Barcelona, Barcelona, Spain, [Salas-Salvado, Jordi] Univ Rovira & Virgili, Hosp Univ St Joan de Reus, Reus, Spain, [Corella, Dolores] Univ Valencia, Valencia, Spain, [Alonso-Gomez, Angel] Univ Basque Country, UPV EHU, Bioaraba Hlth Res Inst, Leioa, Spain, [Angel Martinez-Gonzalez, Miguel] Univ Navarra, Pamplona, Spain, [Angel Martinez-Gonzalez, Miguel] Harvard TH Chan Sch Publ Hlth, Boston, MA USA, [Schroder, Helmut] Inst Salud Carlos III, CIBER Epidemiol & Publ Hlth CIBERESP, Madrid, Spain, [Serra-Majem, Lluis] Univ Las Palmas Gran Canaria, Las Palmas Gran Canaria, Spain, [Fiol, Miguel] Hosp Son Espases, Balearic Isl Hlth Res Inst, Palma De Mallorca, Balearic Island, Spain, [Lapetra, Jose] Dist Sanitario Atenc Primaria Sevilla, Seville, Spain, [Gomez-Gracia, Enrique] Univ Malaga, Malaga, Spain, [Lassale, Camille] UCL, Dept Epidemiol & Publ Hlth, London, England, Beatriu de Pinos postdoctoral programme of the Government of Catalonia's Secretariat for Universities and Research of the Ministry of Economy and Knowledge, Agencia de Gestio d'Ajuts Universitaris i de Recerca AGAUR, Instituto de Salud Carlos III, and FEDER funds

Subjects
Epidemiology, Events, Leisure time, physical activity, 030204 cardiovascular system & hematology, Cohort Studies, chemistry.chemical_compound, 0302 clinical medicine, Risk Factors, Validation, High-density-lipoprotein, Ampk, 0303 health sciences, biology, Cholesterol, Cardiovascular Diseases, lipids (amino acids, peptides, and proteins), Cardiology and Cardiovascular Medicine, Cohort study, Adult, lifestyle, Activity questionnaire, medicine.medical_specialty, HDL function, Cardiovascular health, Physical activity, 03 medical and health sciences, Leisure Activities, Internal medicine, Cholesterylester transfer protein, medicine, Humans, Hdl functionality, Exercise, 030304 developmental biology, business.industry, Protein, Cholesterol, HDL, biomarkers, Lifestyle, Heart-disease, Cholesterol Ester Transfer Proteins, chemistry, Diabetes Mellitus, Type 2, Heart Disease Risk Factors, biology.protein, business, Biomarkers
Abstract

Aims Physical activity has consistently been shown to improve cardiovascular health and high-density lipoprotein-cholesterol levels. However, only small and heterogeneous studies have investigated the effect of exercise on high-density lipoprotein functions. Our aim is to evaluate, in the largest observational study to date, the association between leisure time physical activity and a range of high-density lipoprotein functional traits. Methods The study sample consisted of 296 Spanish adults at high cardiovascular risk. Usual leisure time physical activity and eight measures of high-density lipoprotein functionality were averaged over two measurements, one year apart. Multivariable linear regression models were used to explore the association between leisure time physical activity (exposure) and each high-density lipoprotein functional trait (outcome), adjusted for cardiovascular risk factors. Results Higher levels of leisure time physical activity were positively and linearly associated with average levels over one year of plasma high-density lipoprotein-cholesterol and apolipoprotein A-I, paraoxonase-1 antioxidant activity, high-density lipoprotein capacity to esterify cholesterol and cholesterol efflux capacity in individuals free of type 2 diabetes only. The increased cholesterol esterification index with increasing leisure time physical activity reached a plateau at around 300 metabolic equivalents.min/day. In individuals with diabetes, the relationship with cholesteryl ester transfer protein followed a U-shape, with a decreased cholesteryl ester transfer protein activity from 0 to 300 metabolic equivalents.min/day, but increasing from there onwards. Increasing levels of leisure time physical activity were associated with poorer high-density lipoprotein vasodilatory capacity. Conclusions In a high cardiovascular risk population, leisure time physical activity was associated not only with greater circulating levels of high-density lipoprotein-cholesterol, but also with better markers of high-density lipoprotein functionality, namely cholesterol efflux capacity, the capacity of high-density lipoprotein to esterify cholesterol and paraoxonase-1 antioxidant activity in individuals free of diabetes and lower cholesteryl ester transfer protein activity in individuals with type 2 diabetes.

Published
2019

9. Conversion of waste parasitic insect (Hylobius abietis L.) into antioxidative, antimicrobial and biodegradable films

Author

Demet Erdönmez, Vykintas Baublys, Sonata Kazlauskaitė, Asier M. Salaberria, Jalel Labidi, Murat Kaya, Idris Sargin, Yavuz Selim Cakmak, Povilas Mulerčikas, Sabire Yazıcı Fen-Edebiyat Fakültesi, and [Kaya, Murat -- Sargin, Idris -- Cakmak, Yavuz S.] Aksaray Univ, Dept Biotechnol & Mol Biol, TR-68100 Aksaray, Turkey -- [Mulercikas, Povilas -- Kazlauskaite, Sonata] Aleksandras Stulginskis Univ, Inst Biol & Plant Biotechnol, LT-53361 Kaunas, Lithuania -- [Labidi, Jalel -- Salaberria, Asier M.] Univ Basque Country UPV EHU, Dept Chem & Environm Engn, Biorefinery Proc Res Grp, Plaza Europa 1, Donostia San Sebastian 20018, Spain -- [Erdonmez, Demet] Aksaray Univ, Fac Sci & Letters, Dept Biol, TR-68100 Aksaray, Turkey -- [Baublys, Vykintas] Vytautas Magnus Univ, Dept Biol, LT-44404 Kaunas, Lithuania

Subjects
Chitosan, biology, Chemistry, Materials Science (miscellaneous), media_common.quotation_subject, Chitin, Insect, Biowaste, Environmental Science (miscellaneous), biology.organism_classification, Antimicrobial, Hylobius abietis, Food science, Antioxidant, media_common, Hylobius Abietis
Abstract

WOS: 000469794300001, Hylobius abietis is a plant parasitic insect belonging to the order Coleoptera and which causes severe damages to coniferous forests in Northern and Eastern Europe. This current study is aimed to provide a new viewpoint into the waste of this insect by producing chitosan. Dry insect corpses consisted of 27.9% chitin and 86.2% of the chitin was converted into the chitosan. FT-IR spectra analyses confirmed the purity and the deacetylation degree of the produced chitosan (molecular weight of chitosan; 7.3 kDa). This chitosan exhibited antimicrobial activity against 18 bacterial strains. Further, biodegradable chitosan composite films with beta-carotene were produced. Antioxidant activity of chitosan films were found to be higher than chitosan gels; and beta-carotene incorporation further increased the antioxidative properties of the chitosan films. This study demonstrated that the waste of parasitic insect like H. abietis can be evaluated as a source for production of biodegradable and edible chitosan-based films for applications in food coating.

Published
2019

10. Novel, multifunctional mucilage composite films incorporated with cellulose nanofibers

Author

Muhammad Mujtaba, Lalehan Akyuz, Sami Boufi, Asier Salaberria Martinez, Jalel Labidi, Murat Kaya, Yavuz Selim Cakmak, Behlul Koc, Demet Cansaran-Duman, Sedef Ilk, [Mujtaba, Muhammad -- Cansaran-Duman, Demet] Ankara Univ, Inst Biotechnol, TR-06110 Ankara, Turkey -- [Akyuz, Lalehan] Aksaray Univ, Tech Vocat Sch, Dept Chem Technol, TR-68100 Aksaray, Turkey -- [Boufi, Sami] Univ Sfax, Fac Sci, LMSE, BP 802, Sfax 3018, Tunisia -- [Koc, Behlul -- Kaya, Murat -- Cakmak, Yavuz S.] Aksaray Univ, Fac Sci & Letters, Dept Biotechnol & Mol Biol, TR-68100 Aksaray, Turkey -- [Salaberria Martinez, Asier -- Labidi, Jalel] Univ Basque Country, UPV EHU, Biorefinery Proc Res Grp, Dept Chem & Environm Engn, Plaza Europa 1, Donostia San Sebastian 20018, Spain -- [Ilk, Sedef] Omer Halisdemir Univ, Cent Lab Res Ctr, TR-51240 Nigde, Turkey, Cansaran-Duman, Demet -- 0000-0003-3759-6664, Mujtaba, Muhammad -- 0000-0001-8392-9226, and Aksaray Teknik Bilimler Meslek Yüksekokulu

Subjects
Thermogravimetric analysis, Materials science, General Chemical Engineering, Composite number, Young's modulus, Mechanical properties, 01 natural sciences, symbols.namesake, chemistry.chemical_compound, 0404 agricultural biotechnology, SDG 3 - Good Health and Well-being, 0103 physical sciences, Ultimate tensile strength, Cellulose, Mucilage film, Biological assay, 010304 chemical physics, 04 agricultural and veterinary sciences, General Chemistry, Dynamic mechanical analysis, 040401 food science, Chemical engineering, Mucilage, chemistry, Nanofiber, symbols, Physicochemical characterization, Food Science
Abstract

WOS: 000455346800003, In the current study, mucilage composite films were produced by incorporating the cellulose nanofibrils (CNF) at different concentrations i.e., 3 and 6% to chia mucilage matrix by solution casting method. To evaluate the effect of CNF incorporation into mucilage matrix, the obtained composite films were characterized extensively using both physicochemical and biological tools. FT-IR spectra confirmed the composite formation by broader -OH bond peaks than in the spectrum of mucilage control film and shift of C=O stretching vibration peak lower wave number and widen in the mucilage films including cellulose nanofibers. Cellulose nanofibers did not reveal a significant effect on the thermal properties of the all three composite films and dynamic thermogravimetric values were recorded as similar or equal to 287 degrees C. Atomic force microscopy revealed a homogenous distribution of cellulose nanofibers across the mucilage matrix. Dynamic mechanical analysis revealed that the inclusion of cellulose nanofibers in mucilage film led to an enhancement of the tensile modulus as well as the tensile strength, but without reducing the strain at break of the film. Cellulose nanofibers incorporation significantly enhanced the hydrophobic properties of the composite films. The cytotoxicity assay results demonstrated mucilage composite films were nontoxic and have anticancer properties; reducing the viability of cancer cell line by similar or equal to 20% and retaining the cell viability of epithelial cell line by similar or equal to 90%. Cellulose nanofibers incorporation also significantly enhanced the antioxidant and antimicrobial attributes of the mucilage composite films. Overall, the current study provided an idea regarding the biocompatible, non-toxic, highly antioxidative and antimicrobial biodegradable nature of mucilage-CNF composite films, making it a suitable candidate for food and pharmaceutical industry.

Published
2019

11. An inclusive physicochemical comparison of natural and synthetic chitin films

Author

Jalel Labidi, Asier M. Salaberria, Fatih Duman, Muhammad Mujtaba, Talat Baran, Murat Kaya, Povilas Mulerčikas, Sabire Yazıcı Fen Edebiyat Fakültesi, Mujtaba, Muhammad -- 0000-0001-8392-9226, Labidi, Jalel -- 0000-0002-8382-9492, Duman, Fatih -- 0000-0003-2066-5176, and [Kaya, Murat -- Mujtaba, Muhammad] Aksaray Univ, Fac Sci & Letters, Dept Biotechnol & Mol Biol, TR-68100 Aksaray, Turkey -- [Salaberria, Asier M. -- Labidi, Jalel] Univ Basque Country, UPV EHU, Dept Chem & Environm Engn, Biorefinery Proc Res Grp, Plaza Europa 1, Donostia San Sebastian 20018, Spain -- [Baran, Talat] Aksaray Univ, Fac Sci & Letters, Dept Chem, TR-68100 Aksaray, Turkey -- [Mulercikas, Povilas] Lithuanian Univ Agr, Dept Biol & Plant Protect, LT-53361 Kaunas, Lithuania -- [Duman, Fatih] Erciyes Univ, Fac Sci, Dept Biol, Kayseri, Turkey

Subjects
Materials science, Surface Properties, Nanofibers, Modulus, Nanotechnology, Chitin, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Biochemistry, Contact angle, chemistry.chemical_compound, Structural Biology, Tensile Strength, Ultimate tensile strength, Thermal stability, Elytra, Fourier transform infrared spectroscopy, Molecular Biology, Dissolution, Chitosan, Synthetic film, General Medicine, Carbon-13 NMR, 021001 nanoscience & nanotechnology, Natural film, 0104 chemical sciences, chemistry, Chemical engineering, Synthetic Film, Nanoparticles, 0210 nano-technology, Hydrophobic and Hydrophilic Interactions, Insect, Natural Film
Abstract

WOS: 000417661600120, PubMed: 28842199, Natural and synthetic chitin films, obtained from the same source were produced and their physicochemical properties were examined comparatively. Firstly, natural chitin film was obtained from elytra of an insect (Oryctes nasicornis L) and purity of the obtained chitin film (degree of acetylation: 79 +/- 2%) was demonstrated by solid state C-13 nuclear magnetic resonance (C-13 NMR). Then, the synthetic film was produced by dissolving of natural chitin film in LiCl-DMAc. The obtained natural and synthetic films were characterized by AFM, TGA, DSC, FTIR, mechanical properties, light transmission and contact angle. The analyses result demonstrated that natural chitin film lost very important properties such as high thermal stability, transparency, nanofibrous nature, tensile strength, Young's modulus and hydrophobicity after transforming the synthetic film. (C) 2017 Elsevier B.V. All rights reserved.

Published
2018

12. All Poly(ionic liquid)-Based Block Copolymers by Sequential Controlled Radical Copolymerization of Vinylimidazolium Monomers

Author

Daniel Taton, Daniela Cordella, Anthony Kermagoret, David Mecerreyes, Christophe Detrembleur, Mehmet Isik, Christine Jérôme, Antoine Debuigne, Univ Liege, Dept Chem, CERM, Université de Liège-CERM, Univ Basque Country UPV EHU, Joxe Mari Korta Ctr, POLYMAT, Univ Basque Country, Laboratoire de Chimie des Polymères Organiques (LCPO), Centre National de la Recherche Scientifique (CNRS)-Institut Polytechnique de Bordeaux-Ecole Nationale Supérieure de Chimie, de Biologie et de Physique (ENSCBP)-Université de Bordeaux (UB)-Institut de Chimie du CNRS (INC), Team 1 LCPO : Polymerization Catalyses & Engineering, and Centre National de la Recherche Scientifique (CNRS)-Institut Polytechnique de Bordeaux-Ecole Nationale Supérieure de Chimie, de Biologie et de Physique (ENSCBP)-Université de Bordeaux (UB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut Polytechnique de Bordeaux-Ecole Nationale Supérieure de Chimie, de Biologie et de Physique (ENSCBP)-Université de Bordeaux (UB)-Institut de Chimie du CNRS (INC)

Subjects
Polymers and Plastics, Radical polymerization, Ethyl acetate, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, METATHESIS, Inorganic Chemistry, chemistry.chemical_compound, Polymer chemistry, Materials Chemistry, Copolymer, Vinyl acetate, Reversible addition−fragmentation chain-transfer polymerization, CONDUCTIVITY, VINYL-ACETATE, Alkyl, chemistry.chemical_classification, FRAGMENTATION CHAIN TRANSFER, POLYMERIZED IONIC LIQUIDS, ELECTROLYTES, Organic Chemistry, RAFT POLYMERIZATION, PERFORMANCE, 021001 nanoscience & nanotechnology, ANION-EXCHANGE, 0104 chemical sciences, [CHIM.POLY]Chemical Sciences/Polymers, Monomer, chemistry, Ionic liquid, SEPARATION, 0210 nano-technology
Abstract

International audience; The organometallic-mediated radical polymerization (OMRP) of N-vinyl-3-alkylimidazolium-type monomers, featuring the bis(trifluoromethylsulfonyl)imide counter-anion (Tf2N-) in the presence of Co(acac)(2) as controlling agent, is reported. Polymerizations of monomers with methyl, ethyl, and butyl substituents are fast, reaching high monomer conversion in ethyl acetate as solvent at 30 degrees C, and afford structurally well-defined hydrophobic poly(ionic liquid)s (PILs) of N-vinyl type. Block copolymer synthesis is also achieved by sequential OMRP of N-vinyl-3-alkyldazolium salts carrying different alkyl chains and different counteranions (Tf2N- or Br-). These block copolymerizations are carried out at 30 degrees C, either under homogeneous solution in methanol or in a biphasic medium consisting of a mixture of ethyl acetate and water. Unprecedented PIL-b-PIL block copolymers are thus prepared under these conditions. However, anion exchange occurs at the early stage of the growth of the second block. Finally, diblock copolymers generated in the biphasic medium can be readily coupled by addition of isoprene, forming all PIL-based and symmetrical ABA-type triblock copolymers in a one-pot process. Such a direct block copolymerization method, involving vinylimidazolium monomers bearing different alkyl chains, thus opens new opportunities in the precision synthesis of all PIL-based block copolymers of tunable properties.

Published
2015

13. Non-Isocyanate Polyurethane Soft Nanoparticles Obtained by Surfactant-Assisted Interfacial Polymerization

Author

James L. Hedrick, Daniel Taton, Zhan Yuin Ong, Yi Yan Yang, Gavin O. Jones, Haritz Sardon, Amaury Bossion, David Mecerreyes, Laboratoire de Chimie des Polymères Organiques (LCPO), Centre National de la Recherche Scientifique (CNRS)-Institut Polytechnique de Bordeaux-Ecole Nationale Supérieure de Chimie, de Biologie et de Physique (ENSCBP)-Université de Bordeaux (UB)-Institut de Chimie du CNRS (INC), Team 1 LCPO : Polymerization Catalyses & Engineering, Centre National de la Recherche Scientifique (CNRS)-Institut Polytechnique de Bordeaux-Ecole Nationale Supérieure de Chimie, de Biologie et de Physique (ENSCBP)-Université de Bordeaux (UB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut Polytechnique de Bordeaux-Ecole Nationale Supérieure de Chimie, de Biologie et de Physique (ENSCBP)-Université de Bordeaux (UB)-Institut de Chimie du CNRS (INC), Univ Basque Country UPV EHU, Joxe Mari Korta Ctr, POLYMAT, Univ Basque Country, IBM Almaden Research Center [San Jose], IBM, Departamento de Ciencia y Tecnologıa de Polımeros e Instituto de Materiales Polimericos (POLYMAT), Universidad del Pais Vasco / Euskal Herriko Unibertsitatea [Espagne] (UPV/EHU), and European Commission

Subjects
Materials science, polyurethanes, Nanoparticle, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, chemistry.chemical_compound, nanogels, Polymer chemistry, Electrochemistry, General Materials Science, Spectroscopy, ComputingMilieux_MISCELLANEOUS, Polyurethane, isocyanate free, Surfaces and Interfaces, 021001 nanoscience & nanotechnology, Condensed Matter Physics, Interfacial polymerization, Isocyanate, 0104 chemical sciences, Miniemulsion, Monomer, [CHIM.POLY]Chemical Sciences/Polymers, chemistry, Polymerization, Drug delivery, 0210 nano-technology
Abstract

Polyurethanes (PUs) are considered ideal candidates for drug delivery applications due to their easy synthesis, excellent mechanical properties, and biodegradability. Unfortunately, methods for preparing well-defined PU nanoparticles required miniemulsion polymerization techniques with a nontrivial control of the polymerization conditions due to the inherent incompatibility of isocyanate-containing monomers and water. In this work, we report the preparation of soft PU nanoparticles in a one-pot process using interfacial polymerization that employs a non-isocyanate polymerization route that minimizes side reactions with water. Activated pentafluorophenyl dicarbonates were polymerized with diamines and/or triamines by interfacial polymerization in the presence of an anionic emulsifier, which afforded non-isocyanate polyurethane (NIPU) nanoparticles with sizes in the range of 200–300 nm. Notably, 5 wt % of emulsifier was required in combination with a trifunctional amine to achieve stable PU dispersions and avoid particle aggregation. The versatility of this polymerization process allows for incorporation of functional groups into the PU nanoparticles, such as carboxylic acids, which can encapsulate the chemotherapeutic doxorubicin through ionic interactions. Altogether, this waterborne synthetic method for functionalized NIPU soft nanoparticles holds great promise for the preparation of drug delivery nanocarriers. The authors thank the European Commission for their financial support through the project SUSPOL-EJD 642671 and the Gobierno Vasco/Eusko Jaurlaritza (IT 999-16). Haritz Sardon gratefully acknowledges financial support from MINECO through project SUSPOL and FDI 16507. Yi Yan Yang gratefully acknowledges financial support from the Institute of Bioengineering and Nanotechnology (Biomedical Research Council and Joint Council Office, Agency for Science, Technology and Research, Singapore).

Published
2017

14. Innovative polyelectrolytes/poly(ionic liquid)s for energy and the environment

Author

Ajjan, Fátima, Ambrogi, Martina, Tiruye, Girum Ayalneh, Cordella, Daniel, Fernandes, Ana M, Grygiel, Konrad, Isik, Mehmet, Patil, Nagaraj, Porcarelli, Luca, Rocasalbas, Gillem, Vendramientto, Giordano, Zeglio, Erica, Antonietti, Markus, Detrembleur, Cristophe, Inganäs, Olle, Jérome, Christine, Marcilla, Rebeca, Mecerreyes, David, Moreno, Mónica, Taton, Daniel, Solin, Niclas, Yuan, Jiayin, linkoping university, department of physical chemistry and biology, Department of Colloid Chemistry [Potsdam], Max Planck Institute of Colloids and Interfaces, Max-Planck-Gesellschaft-Max-Planck-Gesellschaft, Institute IMDEA Materials [Madrid], Institute IMDEA Materials, Laboratoire de Chimie des Polymères Organiques (LCPO), Centre National de la Recherche Scientifique (CNRS)-Institut Polytechnique de Bordeaux-Ecole Nationale Supérieure de Chimie, de Biologie et de Physique (ENSCBP)-Université de Bordeaux (UB)-Institut de Chimie du CNRS (INC), Team 1 LCPO : Polymerization Catalyses & Engineering, Centre National de la Recherche Scientifique (CNRS)-Institut Polytechnique de Bordeaux-Ecole Nationale Supérieure de Chimie, de Biologie et de Physique (ENSCBP)-Université de Bordeaux (UB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut Polytechnique de Bordeaux-Ecole Nationale Supérieure de Chimie, de Biologie et de Physique (ENSCBP)-Université de Bordeaux (UB)-Institut de Chimie du CNRS (INC), Centre d'Etude et de Recherche sur les Macromolécules (CERM), Université de Liège, Institue for Polymer Materials (POLYMAT), Université du pays basque, Univ Basque Country UPV EHU, Joxe Mari Korta Ctr, POLYMAT, Univ Basque Country, KIOMedPharma, Department of Physics, Chemistry and Biology [Linköping] (IFM), and Linköping University (LIU)

Subjects
[CHIM.POLY]Chemical Sciences/Polymers, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2017

15. Anti-Inflammatory Effects of the Mediterranean Diet in the Early and Late Stages of Atheroma Plaque Development

Author

Jordi Salas-Salvadó, María P. Portillo, Olga Castañer, Sara Arranz, Rosa Casas, Dolores Corella, Mireia Urpi-Sarda, R. M. Lamuela-Raventos, Emilio Sacanella, Ramon Estruch, José Lapetra, [Casas, Rosa] Univ Barcelona, Hosp Clin, Inst Invest Biomed August Pi & Sunyer IDIBAPS, Villarroel 170, E-08036 Barcelona, Spain, [Sacanella, Emilio] Univ Barcelona, Hosp Clin, Inst Invest Biomed August Pi & Sunyer IDIBAPS, Villarroel 170, E-08036 Barcelona, Spain, [Arranz, Sara] Univ Barcelona, Hosp Clin, Inst Invest Biomed August Pi & Sunyer IDIBAPS, Villarroel 170, E-08036 Barcelona, Spain, [Estruch, Ramon] Univ Barcelona, Hosp Clin, Inst Invest Biomed August Pi & Sunyer IDIBAPS, Villarroel 170, E-08036 Barcelona, Spain, [Casas, Rosa] Inst Salud Carlos III, Ciber Fisiopatol Obesidad & Nutr CIBEROBN, Madrid, Spain, [Sacanella, Emilio] Inst Salud Carlos III, Ciber Fisiopatol Obesidad & Nutr CIBEROBN, Madrid, Spain, [Arranz, Sara] Inst Salud Carlos III, Ciber Fisiopatol Obesidad & Nutr CIBEROBN, Madrid, Spain, [Corella, Dolores] Inst Salud Carlos III, Ciber Fisiopatol Obesidad & Nutr CIBEROBN, Madrid, Spain, [Castaner, Olga] Inst Salud Carlos III, Ciber Fisiopatol Obesidad & Nutr CIBEROBN, Madrid, Spain, [Lamuela-Raventos, Rosa-Maria] Inst Salud Carlos III, Ciber Fisiopatol Obesidad & Nutr CIBEROBN, Madrid, Spain, [Salas-Salvado, Jordi] Inst Salud Carlos III, Ciber Fisiopatol Obesidad & Nutr CIBEROBN, Madrid, Spain, [Lapetra, Jose] Inst Salud Carlos III, Ciber Fisiopatol Obesidad & Nutr CIBEROBN, Madrid, Spain, [Portillo, Maria P.] Inst Salud Carlos III, Ciber Fisiopatol Obesidad & Nutr CIBEROBN, Madrid, Spain, [Estruch, Ramon] Inst Salud Carlos III, Ciber Fisiopatol Obesidad & Nutr CIBEROBN, Madrid, Spain, [Urpi-Sarda, Mireia] Univ Barcelona, Sch Pharm, Dept Nutr & Food Sci, Av Joan XXIII S-N, E-08028 Barcelona, Spain, [Lamuela-Raventos, Rosa-Maria] Univ Barcelona, Sch Pharm, Dept Nutr & Food Sci, Av Joan XXIII S-N, E-08028 Barcelona, Spain, [Corella, Dolores] Univ Valencia, Sch Med, Dept Epidemiol, Valencia, Spain, [Corella, Dolores] Univ Valencia, Sch Med, Dept Biochem & Mol Biol, Valencia, Spain, [Castaner, Olga] Hosp Mar Med Res Inst IMIM, Cardiovasc Risk & Nutr & REGICOR Res Grp, Barcelona, Spain, [Salas-Salvado, Jordi] Univ Rovira & Virgili, Hosp Univ Sant Joan Reus, IISPV, Human Nutr Unit, Reus, Spain, [Lapetra, Jose] Distrito Sanitario Atenc Primaria Sevilla, Dept Family Med, Res Unit, Seville, Spain, [Portillo, Maria P.] Univ Basque Country, UPV EHU, Lucio Lascaray Res Ctr, Dept Nutr & Food Sci,Nutr & Obes Grp, Vitoria, Spain, Instituto de Salud Carlos III, Spain, 'Ramon y Cajal' program of MINECO, Fondo Social Europeo, Universitat de Barcelona, Alimentació, Nutrició, Creixement i Salut Mental, Bioquímica i Biotecnologia, and Universitat Rovira i Virgili

Subjects
Male, 0301 basic medicine, Pathology, Mediterranean diet, High-risk, 030204 cardiovascular system & hematology, Diet, Mediterranean, Dieta mediterrània, Gastroenterology, 0302 clinical medicine, Risk Factors, Elderly people, Chemokine CCL4, Chemokine CCL5, Diet, Fat-Restricted, Aged, 80 and over, Bioquímica y tecnología, Interleukin-18, Middle Aged, Inflammatory biomarkers, Plaque, Atherosclerotic, Interleukin-10, Lipoproteins, LDL, Biochemistry and technology, Cardiovascular diseases, Cardiovascular Diseases, Plasma concentration, Cytokines, Female, lcsh:RB1-214, medicine.medical_specialty, Article Subject, medicine.drug_class, Immunology, Vascular Cell Adhesion Molecule-1, Activation, Primary care, Bioquímica i biotecnologia, Low-grade inflammation, Anti-inflammatory, 03 medical and health sciences, Mediterranean cooking, Elderly persons, Cardiovascular-disease, Internal medicine, Cuina mediterrània, lcsh:Pathology, medicine, Humans, Aged, Markers, 030109 nutrition & dietetics, Interleukin-6, Malalties cardiovasculars, business.industry, Pattern, Cell Biology, medicine.disease, Atherosclerosis, Atheroma, Clinical Study, 0962-9351, Placa ateroscleròtica, business
Abstract

Objective.To evaluate the long-term effects of a Mediterranean diet (MeDiet) intervention on the plasma concentrations of inflammatory and plaque stability-related molecules in elderly people at high risk for cardiovascular disease.Design and Setting. 66 participants from primary care centers affiliated with the Hospital Clinic of Barcelona were randomized into 3 groups: MeDiet plus extra virgin olive oil (EVOO) or nuts and a low-fat diet (LFD). At baseline and at 3 and 5 years, we evaluated the changes in the plasma concentrations of 24 inflammatory biomarkers related to the different stages of the atherosclerotic process by Luminex®.Results.At 3 and 5 years, both MeDiet groups showed a significant reduction of IL-6, IL-8, MCP-1, and MIP-1β(P<0.05; all) compared to LFD. IL-1β, IL-5, IL-7, IL-12p70, IL-18, TNF-α, IFN-γ, GCSF, GMCSF, and ENA78 (P<0.05; all) only decreased in the MeDiet+EVOO group and E-selectin and sVCAM-1 (P<0.05; both) in the MeDiet+nuts group.Conclusions. Long-term adherence to MeDiet decreases the plasma concentrations of inflammatory biomarkers related to different steps of atheroma plaque development in elderly persons at high cardiovascular risk.

Published
2017
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16. Looking beyond the veil of the European crisis : the need to uncover the structural causes of health inequalities

Author

Amaia Bacigalupe, Miguel San Sebastian, Antonio Escolar Pujolar, [Escolar Pujolar, Antonio] Delegac Terr Igualdad Salud & Polit Sociales, Cadiz, Spain, [Bacigalupe, Amaia] Univ Basque Country UPV EHU, Dept Sociol, Leioa, Spain, and [San Sebastian, Miguel] Umea Univ, Dept Clin Med & Publ Hlth, Epidemiol & Global Hlth, Umea, Sweden

Subjects
Economic recession, medicine.medical_specialty, Inequality, media_common.quotation_subject, Public administration, Recession, 03 medical and health sciences, Self-rated health, 0302 clinical medicine, Political science, Development economics, medicine, Humans, 030212 general & internal medicine, Health policy, media_common, Social policy, 030505 public health, Public health, Health Policy, Public Health, Environmental and Occupational Health, Health services research, Public Health, Global Health, Social Medicine and Epidemiology, Health Status Disparities, Folkhälsovetenskap, global hälsa, socialmedicin och epidemiologi, Impact, Economic Recession, Spain, Commentary, Root Cause Analysis, 0305 other medical science, Root cause analysis
Abstract

Looking beyond the veil of the European crisis : the need to uncover the structural causes of health inequalities

Published
2016

17. Synthesis of Polyurethanes Using Organocatalysis: A Perspective

Author

David Mecerreyes, Daniel Taton, Haritz Sardon, Henri Cramail, James L. Hedrick, Ana Pascual, Univ Basque Country UPV EHU, Joxe Mari Korta Ctr, POLYMAT, Univ Basque Country, Basque Fdn Sci, Ikerbasque, IKERBASQUE, Basque Foundation for Science, Laboratoire de Chimie des Polymères Organiques (LCPO), Centre National de la Recherche Scientifique (CNRS)-Institut Polytechnique de Bordeaux-Ecole Nationale Supérieure de Chimie, de Biologie et de Physique (ENSCBP)-Université de Bordeaux (UB)-Institut de Chimie du CNRS (INC), Team 1 LCPO : Polymerization Catalyses & Engineering, Centre National de la Recherche Scientifique (CNRS)-Institut Polytechnique de Bordeaux-Ecole Nationale Supérieure de Chimie, de Biologie et de Physique (ENSCBP)-Université de Bordeaux (UB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut Polytechnique de Bordeaux-Ecole Nationale Supérieure de Chimie, de Biologie et de Physique (ENSCBP)-Université de Bordeaux (UB)-Institut de Chimie du CNRS (INC), Team 2 LCPO : Biopolymers & Bio-sourced Polymers, IBM Almaden Research Center [San Jose], and IBM

Subjects
Polymers and Plastics, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Ring-opening polymerization, Dibutyltin dilaurate, Catalysis, Inorganic Chemistry, 6-MEMBERED CYCLIC CARBONATES, chemistry.chemical_compound, ALIPHATIC POLYURETHANES, N-HETEROCYCLIC CARBENES, Materials Chemistry, Organic chemistry, Polyurethane, GAUSSIAN-TYPE BASIS, CATALYST-FREE SYNTHESIS, Organic base, Chemistry, Organic Chemistry, THERMOPLASTIC POLYURETHANES, 021001 nanoscience & nanotechnology, Isocyanate, SPECTROPHOTOMETRIC BASICITY SCALE, 0104 chemical sciences, MOLECULAR-ORBITAL METHODS, RING-OPENING POLYMERIZATION, [CHIM.POLY]Chemical Sciences/Polymers, Polymerization, Organocatalysis, NON-ISOCYANATE POLYURETHANES, 0210 nano-technology
Abstract

International audience; Organocatalysis has become an invaluable tool for polymer synthesis, and its utility has been demonstrated in ring-opening, anionic, zwitterionic, and group-transfer polymerizations. Despite this, the use of organocatalysis in other polymerization reactions such as step-growth polymerizations remains underexplored, relative to more traditional metal-based polymerizations. Recently, the use of organic bases such as guanidines, amidines, N-heterocyclic carbenes, and organic "strong or super-strong" Bronsted acids to catalyze the synthesis of metal-free polyurethanes has shown to be competitive to commercially widely used dibutyltin dilaurate and dibutyltin diacetate catalysts. This Perspective article highlights recent advances in organocatalyst design for isocyanate-based polyurethane synthesis with the aim of comparing the activity and selectivity of each of the new catalytic reactions to each other and the traditional metal-based catalysts. The article also draws attention to new trends in isocyanate-free polyurethane synthesis and the key role that organocatalysis is playing in these innovative polymerization processes.

Published
2015

18. Fully Printed Electrodes on Stretchable Textiles for Long-Term Electrophysiology

Author

Thierry Hervé, Ana Sanchez-Sanchez, Eloise Bihar, George G. Malliaras, David Mecerreyes, Esma Ismailova, Timothée Roberts, Mehmet Isik, Mohamed Saadaoui, Jozina B. De Graaf, Institut des Sciences du Mouvement Etienne Jules Marey (ISM), Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Ecole Nationale Supérieure des Mines de Saint-Etienne, Centre Microélectronique de Provence (EMSE-CMP), Ecole Nationale Supérieure des Mines de Saint-Etienne, Centre Microélectronique de Provenc (EMSE-CMP), Ministère du Redressement productif-Ministère du Redressement productif, EMSE-CMP, Univ Basque Country UPV EHU, Joxe Mari Korta Ctr, POLYMAT, Univ Basque Country, Ecole Natl Super Mines, Dept Bioelect, Ecole Natl Super Mines Gardanne, and Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Conductive polymer, Textile, Materials science, business.industry, [SDV]Life Sciences [q-bio], education, technology, industry, and agriculture, Cardiac activity, Nanotechnology, 02 engineering and technology, 010402 general chemistry, 021001 nanoscience & nanotechnology, 01 natural sciences, Industrial and Manufacturing Engineering, 0104 chemical sciences, [SPI]Engineering Sciences [physics], chemistry.chemical_compound, chemistry, Mechanics of Materials, Electrode, Ionic liquid, General Materials Science, 0210 nano-technology, business, Textile electrodes
Abstract

International audience; Fully printed electrodes consisting of a conducting polymer and an ionic liquid gel are fabricated on a stretchable textile. They are shown to record cardiac activity while the wearer is moving and for long periods of time, paving the way for the development of low-cost devices for continuous health monitoring.

Published
2017

19. Post-polymerization modification and organocatalysis using reactive statistical poly(ionic liquid)-based copolymers

Author

Paul Coupillaud, David Mecerreyes, Joan Vignolle, Daniel Taton, Laboratoire de Chimie des Polymères Organiques (LCPO), Centre National de la Recherche Scientifique (CNRS)-Institut Polytechnique de Bordeaux-Ecole Nationale Supérieure de Chimie, de Biologie et de Physique (ENSCBP)-Université de Bordeaux (UB)-Institut de Chimie du CNRS (INC), Univ Basque Country UPV EHU, Joxe Mari Korta Ctr, POLYMAT, Univ Basque Country, Team 1 LCPO : Polymerization Catalyses & Engineering, and Centre National de la Recherche Scientifique (CNRS)-Institut Polytechnique de Bordeaux-Ecole Nationale Supérieure de Chimie, de Biologie et de Physique (ENSCBP)-Université de Bordeaux (UB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut Polytechnique de Bordeaux-Ecole Nationale Supérieure de Chimie, de Biologie et de Physique (ENSCBP)-Université de Bordeaux (UB)-Institut de Chimie du CNRS (INC)

Subjects
STRUCTURAL-CHARACTERIZATION, Materials science, Polymers and Plastics, CATALYSTS, HYDROGEN CARBONATES, Catalysis, Styrene, chemistry.chemical_compound, Deprotonation, Post-polymerization modification, Polymer chemistry, N-HETEROCYCLIC CARBENES, Materials Chemistry, Copolymer, Propylene oxide, Benzoin condensation, Poly(ionic liquid), MECHANISTIC INSIGHT, Organic Chemistry, CYCLIC CARBONATES, [CHIM.POLY]Chemical Sciences/Polymers, chemistry, IONIC LIQUIDS, Ionic liquid, COMPLEXES, POLYMERS, EPOXIDES, Carbene
Abstract

International audience; Copoly(ionic liquid)s (coPILs) based on poly(styrene)-co-poly(4-vinylbenzylbutylimidazolium) with different anions (Cl- and HCO3-), denoted as PS-co-PVBnBuImCl 1 and PS-co-PVBnBuImHCO(3) 2, were used as reactive polymers for the purpose of post-polymerization modification and organic catalysis. While coPIL 1 could be derived into the corresponding poly(N-heterocyclic carbene)-silver transition metal complex referred to as poly(NHC-Ag) by a simple deprotonation/metallation sequence utilizing Ag2O, coPIL 2 was found to quantitatively react with various electrophiles, including CS2, isothiocyanate and transition metals (based on Pd and Au) upon formal loss of "H2CO3, affording the post-functionalized poly(NHC-CS2), poly(NHC-isothiocyanate) and poly(NHC-Met) (Met = Au, Pd) copolymers. The catalytic activity of both coPILs 1 and 2 was also examined in cyclic carbonate formation by reaction between CO2 and propylene oxide and in the benzoin condensation, respectively. (C) 2014 Elsevier Ltd. All rights reserved.

Published
2014

20. Precision Synthesis of Poly(Ionic Liquid)-Based Block Copolymers by Cobalt-Mediated Radical Polymerization and Preliminary Study of Their Self-Assembling Properties

Author

Anne-Laure Wirotius, Paul Coupillaud, Joan Vignolle, Guillaume Fleury, Christophe Detrembleur, David Mecerreyes, Antoine Debuigne, Karim Aissou, Mareva Fevre, Daniel Taton, Laboratoire de Chimie des Polymères Organiques (LCPO), Centre National de la Recherche Scientifique (CNRS)-Institut Polytechnique de Bordeaux-Ecole Nationale Supérieure de Chimie, de Biologie et de Physique (ENSCBP)-Université de Bordeaux (UB)-Institut de Chimie du CNRS (INC), Univ Basque Country UPV EHU, Joxe Mari Korta Ctr, POLYMAT, Univ Basque Country, Team 4 LCPO : Polymer Materials for Electronic, Energy, Information and Communication Technologies, Centre National de la Recherche Scientifique (CNRS)-Institut Polytechnique de Bordeaux-Ecole Nationale Supérieure de Chimie, de Biologie et de Physique (ENSCBP)-Université de Bordeaux (UB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut Polytechnique de Bordeaux-Ecole Nationale Supérieure de Chimie, de Biologie et de Physique (ENSCBP)-Université de Bordeaux (UB)-Institut de Chimie du CNRS (INC), Univ Liege, Dept Chem, CERM, Université de Liège-CERM, and Team 1 LCPO : Polymerization Catalyses & Engineering

Subjects
CMRP, poly(ionic liquid), Materials science, Polymers and Plastics, Free Radicals, Polymers, Radical polymerization, mesostructure, Ionic Liquids, THERMAL-PROPERTIES, block copolymer, TRIBLOCK COPOLYMERS, chemistry.chemical_compound, Dynamic light scattering, Lamellar phase, Polymer chemistry, Materials Chemistry, Copolymer, SCATTERING, Lamellar structure, CONDUCTIVITY, IONIC LIQUID, MICELLES, Small-angle X-ray scattering, Organic Chemistry, Water, Cobalt, self-assembly, DIFFUSION, ANION-EXCHANGE, DIBLOCK COPOLYMERS, [CHIM.POLY]Chemical Sciences/Polymers, chemistry, Cobalt-mediated radical polymerization, Ionic liquid, ORDERED PHASE-SEPARATION
Abstract

International audience; A poly(ionic liquid)-based block copolymer (PIL BCP), namely, poly(vinyl acetate)-b-poly(N-vinyl-3-butylimidazolium bromide), PVAc-b-PVBuImBr, is synthesized by sequential cobalt-mediated radical polymerization (CMRP). A PVAc precursor is first prepared at 30 degrees C in bulk by CMRP of VAc, using bis(acetylacetonato)cobalt(II), Co(acac)(2), and a radical source (V-70). Growth of PVBuImBr from PVAc-Co(acac)(2) is accomplished by CMRP in DMF/MeOH (2:1, v/v). This PIL BCP self-assembles in the sub-micron size range into aggregated core-shell micelles in THF, whereas polymeric vesicles are observed in water, as evidenced by dynamic light scattering (DLS) and transmission electron microscopy (TEM). Thin-solid sample cut from raw materials analyzed by TEM shows an ordered lamellar organization by temperature-dependent synchrotron small-angle X-ray scattering (SAXS). Anion exchange can be accomplished to achieve the corresponding PIL BCP with bis(trifluorosulfonyl)imide (Tf2N-) anions, which also gives rise to an ordered lamellar phase in bulk samples. A complete suppression of SAXS second-order reflection suggests that this compound has a symmetric volume fraction (f approximate to 0.5). SAXS characterization of both di- and triblock PIL BCP analogues previously reported also shows a lamellar phase of very similar behavior, with only an increase of the period by about 8% at 60 degrees C.

Published
2014

21. Direct Route to Well-Defined Poly(ionic liquid)s by Controlled Radical Polymerization in Water

Author

Christophe Detrembleur, Daniel Taton, Christine Jérôme, Ian German, Mehmet Isik, Raphaël Riva, Antoine Debuigne, Anthony Kermagoret, David Mecerreyes, Daniela Cordella, Univ Liege, Dept Chem, CERM, Université de Liège-CERM, Univ Basque Country UPV EHU, Joxe Mari Korta Ctr, POLYMAT, Univ Basque Country, Laboratoire de Chimie des Polymères Organiques (LCPO), Centre National de la Recherche Scientifique (CNRS)-Institut Polytechnique de Bordeaux-Ecole Nationale Supérieure de Chimie, de Biologie et de Physique (ENSCBP)-Université de Bordeaux (UB)-Institut de Chimie du CNRS (INC), Team 1 LCPO : Polymerization Catalyses & Engineering, and Centre National de la Recherche Scientifique (CNRS)-Institut Polytechnique de Bordeaux-Ecole Nationale Supérieure de Chimie, de Biologie et de Physique (ENSCBP)-Université de Bordeaux (UB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut Polytechnique de Bordeaux-Ecole Nationale Supérieure de Chimie, de Biologie et de Physique (ENSCBP)-Université de Bordeaux (UB)-Institut de Chimie du CNRS (INC)

Subjects
AMBIENT-TEMPERATURE, Polymers and Plastics, METHACRYLATE, Radical polymerization, Dispersity, CLICK CHEMISTRY POLYADDITION, SOLUBLE (CO)POLYMERS, AQUEOUS RAFT POLYMERIZATION, Inorganic Chemistry, chemistry.chemical_compound, Polymer chemistry, Materials Chemistry, Reversible addition−fragmentation chain-transfer polymerization, VINYL-ACETATE, IONIC LIQUID, Molar mass, Chemistry, HYDROPHILIC BLOCK-COPOLYMERS, Organic Chemistry, DIBLOCK COPOLYMERS, [CHIM.POLY]Chemical Sciences/Polymers, Monomer, Cobalt-mediated radical polymerization, N-BUTYL ACRYLATE, Ionic liquid, Ionic polymerization
Abstract

International audience; The precision synthesis of poly(ionic liquid)s (PILs) in water is achieved for the first time by the cobalt-mediated radical polymerization (CMRP) of N-vinyl-3-alkylimidazolium-type monomers following two distinct protocols. The first involves the CMRP of various 1-vinyl-3-alkylimidazolium bromides conducted in water in the presence of an alkyl cobalt(III) complex acting as a monocomponent initiator and mediating agent. Excellent control over molar mass and dispersity is achieved at 30 degrees C. Polymerizations are complete in a few hours, and PIL chain-end fidelity is demonstrated up to high monomer conversions. The second route uses the commercially available bis(acetylacetonato)cobalt(II) (Co(acac)2) in conjunction with a simple hydroperoxide initiator (tertbutyl hydroperoxide) at 30, 40, and 50 degrees C in water, facilitating the scaling-up of the technology. Both routes prove robust and straightforward, opening new perspectives onto the tailored synthesis of PILs under mild experimental conditions in water.

Published
2014

22. Time-course distribution of fluorescent microplastics in target tissues of mussels and polychaetes.

Author

Calmão M, Blasco N, Benito A, Thoppil R, Torre-Fernandez I, Castro K, Izagirre U, Garcia-Velasco N, and Soto M

Abstract

The majority of the plastic produced in the last century is accumulated in the environment, leading to an exacerbated contamination of marine environments due to transport from land to the ocean. In the ocean, mechanical abrasion, oxidation, and photodegradation degrade large plastics into microplastics (MPs) - 0.1 μm to 5 mm (EFSA, 2016) which are transported through water currents reaching the water surface, water column, and sediments. Further, they can be accumulated by aquatic and benthic species, entering the trophic chain and becoming a potential threat to humans. In the present research, we aimed to decipher the accumulation and distribution time-courses between different organs or target tissues of organisms inhabiting coastal areas such as mussels Mytilus galloprovincialis and polychaetes Hediste diversicolor. Both were exposed in microcosm experiments to fluorescent polystyrene MPs (1 μm) which were spiked at two doses (10 3 and 10 5 particles/mL) for 1, 4, 24, and 72 h. Mussels and polychaetes were digested with 10% KOH and filtered to quantify the number of MPs incorporated. Different anatomical parts of the body were selected and processed for cryosectioning and posterior microscopic localisation of MPs. Both species accumulate MPs spiked in water column, mainly after exposure to the highest dose. In mussels, particles were found in distinct parts of the digestive tract (stomach, digestive diverticula, ducts) and gills. Even if the majority of MPs were localised in the lumen of the digestive tract, in some cases, were inside the digestive epithelium. The identification of MPs and their internalization in the digestive system was studied using Raman spectroscopy. A decreasing trend with time regarding MPs number in the digestive tract (stomach) of mussels was observed while the opposite was recorded for polychaetes and sediments. The combination of microscopical observations of frozen sections and Raman, appeared to be accurate methodologies to address MPs abundances and to reveal their localisation in different organs. This work has enabled to understand the distribution and fate of MPs in different environmental compartments and it could contribute to gain knowledge about their impact after ingestion by coastal organisms., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 The Authors. Published by Elsevier Ltd.. All rights reserved.)

Published
2023
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23. Reversible and Non-Reversible Transformation of Magnetic Structure in Amorphous Microwires.

Author

Chizhik A, Gonzalez J, Zhukov A, Gawronski P, Ipatov M, Corte-León P, Blanco JM, and Zhukova V

Abstract

We provide an overview of the tools directed to reversible and irreversible transformations of the magnetic structure of glass-covered microwires. The irreversible tools are the selection of the chemical composition, geometric ratio, and the stress-annealing. For reversible tuning we use the combination of magnetic fields and mechanical stresses. The studies were focused on the giant magnetoimpedance effect and the velocity of the domain walls propagation important for the technological applications. The essential increase of the giant magnetoimpedance effect and the control of the domain wall velocity were achieved as a result of the use of two types of control tools. The performed simulations reflect the real transformation of the helical domain structures experimentally found.

Published
2020
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24. Influence of season-depending ecological variables on biomarker baseline levels in mussels (Mytilus trossulus) from two Baltic Sea subregions.

Author

Benito D, Ahvo A, Nuutinen J, Bilbao D, Saenz J, Etxebarria N, Lekube X, Izagirre U, Lehtonen KK, Marigómez I, Zaldibar B, and Soto M

Subjects
Animals, Baltic States, Biomarkers metabolism, Pilot Projects, Water Pollutants, Chemical standards, Environmental Monitoring, Mytilus physiology, Water Pollutants, Chemical metabolism
Abstract

For reliable mussel monitoring programmes based on biomarkers, regionally relevant reference values and their natural variability need to be known. The Baltic Sea exhibits high inter-regional and seasonal variability in physical factors such as salinity, temperature and primary production. The aim of this pilot study is to depict the effects of season-related environmental factors in a selected battery of biomarkers in two environmentally different subregions of the Baltic Sea to help establishing reference data for biochemical, cellular and tissue-level biomarkers. In order to achieve that, mussels were collected from reference sites in Kiel (Germany) and Tvärminne (Finland) during three seasons: summer and autumn 2016, and spring 2017. Finally, in order to characterize the ecological situation, analysis of the chemical tissue burden was performed and chlorophyll‑a and particulate organic carbon concentration and temperature changes were analyzed at each sampling locality using satellite remote sensing images. An integrated biomarker response index was performed to summarize the biomarker responses of each locality and season. The biochemical endpoints showed seasonal variability regulated by temperature, food supply and reproductive cycle, while among the cellular endpoints only lipofuscin accumulation and lysosomal structural changes showed slight seasonal variation. Seasonal changes in tissue level biomarkers were observed only at the northern Baltic Sea site Tvärminne, dictated by the demanding energetic trade-off caused by reproduction. In conclusion, the characterization of the ecological variables and physico-chemical conditions at each site, is crucial to perform a reliable assessment of the effects of a hypothetical pollution scenario in the Baltic Sea. Moreover, reference levels of biomarkers and their responses to natural environmental conditions must be established., (Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.)

Published
2019
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25. Assessment of ecosystem health disturbance in mangrove-lined Caribbean coastal systems using the oyster Crassostrea rhizophorae as sentinel species.

Author

Aguirre-Rubí J, Luna-Acosta A, Ortiz-Zarragoitia M, Zaldibar B, Izagirre U, Ahrens MJ, Villamil L, and Marigómez I

Subjects
Animals, Biomarkers analysis, Caribbean Region, Colombia, Nicaragua, Sentinel Species, Crassostrea drug effects, Ecosystem, Environmental Monitoring, Water Pollutants, Chemical analysis
Abstract

This investigation was aimed at contributing to develop a suitable multi-biomarker approach for pollution monitoring in mangrove-lined Caribbean coastal systems using as sentinel species, the mangrove cupped oyster, Crassostrea rhizophorae. A pilot field study was carried out in 8 localities (3 in Nicaragua; 5 in Colombia), characterized by different environmental conditions and subjected to different levels and types of pollution. Samples were collected in the rainy and dry seasons of 2012-2013. The biological effects at different levels of biological complexity (Stress-on-Stress response, reproduction, condition index, tissue-level biomarkers and histopathology) were determined as indicators of health disturbance, integrated as IBR/n index, and compared with tissue burdens of contaminants in order to achieve an integrative biomonitoring approach. Though modulated by natural variables and confounding factors, different indicators of oyster health, alone and in combination, were related to the presence of different profiles and levels of contaminants present at low-to-moderate levels. Different mixtures of persistent (As, Cd, PAHs) and emerging chemical pollutants (musk fragrances), in combination with different levels of organic and particulate matter resulting from seasonal oceanographic variability and sewage discharges, and environmental factors (salinity, temperature) elicited a different degree of disturbance in ecosystem health condition, as reflected in sentinel C. rhizophorae. As a result, IBR/n was correlated with pollution indices, even though the levels of biological indicators of health disturbance and pollutants were low-to-moderate, and seasonality and the incidence of confounding factors were remarkable. Our study supports the use of simple methodological approaches to diagnose anomalies in the health status of oysters from different localities and to identify potential causing agents and reflect disturbances in ecosystem health. Consequently, the easy methodological approach used herein is useful for the assessment of health disturbance in a variety of mangrove-lined Caribbean coastal systems using mangrove cupped oysters as sentinel species., (Copyright © 2017 Elsevier B.V. All rights reserved.)

Published
2018
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26. Integrative biomarker assessment of the effects of chemically and mechanically dispersed crude oil in Pacific oysters, Crassostrea gigas.

Author

Luna-Acosta A, Bustamante P, Thomas-Guyon H, Zaldibar B, Izagirre U, and Marigómez I

Subjects
Animals, Biomarkers analysis, Crassostrea chemistry, Petroleum analysis, Petroleum Pollution, Water Pollutants, Chemical analysis
Abstract

The impact of dispersed crude oil and dispersant on adult Pacific oysters, Crassostrea gigas, was evaluated through an integrative biomarker approach including (1) biochemical (plasma catecholase- and laccase-type phenoloxidase and superoxide dismutase), (2) histological (digestive cell lysosomal responses, digestive gland histopathology) and (3) physiological (flesh condition index) endpoints in the haemolymph and digestive gland. Adult oysters were exposed to non-contaminated water (control), chemically-dispersed oil (Brut Arabian Light), mechanically-dispersed oil and dispersant (FINASOL®) alone for 2days, and further depurated in non-contaminated water for 4weeks. After exposure to chemically and mechanically dispersed oil oysters exhibited induction of plasma laccase-type phenoloxidase and superoxide dismutase activities, enlargement of digestive cell lysosomes, lipofuscin accumulation, reduced neutral lipid content and atrophy of digestive gland diverticula; more markedly on exposure to chemically dispersed oil. From the studied biomarkers, only lysosomal biomarkers were significantly affected after exposure to the dispersant alone. This included lysosomal enlargement, neutral lipid depletion and lipofuscin accumulation in the digestive gland epithelium. A recovery of plasma enzyme activities was observed after 4weeks of depuration. The integrative biological response index indicated that chemically dispersed oil caused significantly higher stress to C. gigas than the mechanically-dispersed one or the dispersant alone; nevertheless, the response seems to be reversible after depuration., (Copyright © 2017 Elsevier B.V. All rights reserved.)

Published
2017
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27. Combined use of native and caged mussels to assess biological effects of pollution through the integrative biomarker approach.

Author

Marigómez I, Zorita I, Izagirre U, Ortiz-Zarragoitia M, Navarro P, Etxebarria N, Orbea A, Soto M, and Cajaraville MP

Subjects
Animals, Atlantic Ocean, Environmental Monitoring statistics & numerical data, Lipid Peroxidation, Lipofuscin analysis, Metals, Heavy analysis, Micronucleus Tests, Phthalic Acids analysis, Polychlorinated Biphenyls analysis, Polycyclic Aromatic Hydrocarbons analysis, Spain, Biomarkers metabolism, Bivalvia drug effects, Environmental Monitoring methods, Water Pollutants, Chemical analysis, Water Pollutants, Chemical toxicity
Abstract

Native and caged mussels were used in combination for the monitoring of pollution biological effects through an integrative biomarker approach. Mussels (Mytilus galloprovincialis) were deployed in cages in two well-known model localities with different pollution levels in the Basque coast. After 3 weeks caged and native mussels were collected from each site and a suite of effect and exposure biomarkers (from molecular/cellular to organism level) was applied and chemical contaminants (metals, PAHs, PCBs, phthalates and nonylphenol ethoxylates) were analytically determined. Integrative biomarker indices and pollutant indices of tissues were calculated. Several biomarkers used herein responded similarly in native and caged mussels, whereas others exhibited significant differences. Overall, biomarkers in-a-suite depicted site-specific profiles useful for the diagnostic of mussel health status and therefore for ecosystem health assessment in marine pollution biomonitoring. On the other hand, biomarkers and bioaccumulation exhibited different response times, which was especially evident when comparing biomarker and pollutant indices of tissues. The suite of biomarkers was more sensitive after caging (short-term response), whereas tissue pollutant concentrations were more sensitive in native mussels (long-term response). Thus, the combination of native and caged mussels is highly recommended to monitor biological effects of pollution in mussels through the integrative biomarker approach, especially in chronically polluted sites., (Copyright © 2013 Elsevier B.V. All rights reserved.)

Published
2013
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