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1. NMR metabolite quantification of a synthetic urine sample: an inter-laboratory comparison of processing workflows

Author: Canlet, Cécile, DEBORDE, Catherine, Cahoreau, Edern, da Costa, Grégory, Gautier, Roselyne, Jacob, Daniel, Jousse, Cyril, Lacaze, Mélia, Le Mao, Inès, Martineau, Estelle, Peyriga, Lindsay, Richard, Tristan, Silvestre, Virginie, Traïkia, Mounir, Moing, Annick, Giraudeau, Patrick, ToxAlim (ToxAlim), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Ecole d'Ingénieurs de Purpan (INP - PURPAN), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), MetaboHUB-MetaToul, MetaboHUB-Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Metatoul AXIOM (E20 ), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-MetaboHUB-Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-ToxAlim (ToxAlim), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Ecole d'Ingénieurs de Purpan (INP - PURPAN), Biologie du fruit et pathologie (BFP), Université de Bordeaux (UB)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), MetaboHUB-Bordeaux, MetaboHUB, Plateforme Bordeaux Metabolome, Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-MetaboHUB-Bordeaux, MetaboHUB-MetaboHUB, Toulouse Biotechnology Institute (TBI), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), MetaToul FluxoMet (TBI-MetaToul), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Toulouse Biotechnology Institute (TBI), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité de Recherche Œnologie [Villenave d'Ornon] (OENO), Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut de Chimie de Clermont-Ferrand (ICCF), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA)-Institut national polytechnique Clermont Auvergne (INP Clermont Auvergne), Université Clermont Auvergne (UCA)-Université Clermont Auvergne (UCA), MetaboHUB-Clermont, Plateforme Exploration du Métabolisme (PFEM), MetaboHUB-MetaboHUB-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), CAPACITÉS SAS, Nantes Université (Nantes Univ), Chimie Et Interdisciplinarité : Synthèse, Analyse, Modélisation (CEISAM), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Nantes université - UFR des Sciences et des Techniques (Nantes univ - UFR ST), Nantes Université - pôle Sciences et technologie, Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ)-Nantes Université - pôle Sciences et technologie, Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ), MetaboHUB-Grand-Ouest, ANR-11-INBS-0010,METABOHUB,Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation(2011), and European Project: 814747,SUMMIT
Subjects: Synthetic urine, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, [SDV]Life Sciences [q-bio], Peak integration, Metabolomic profiling, Deconvolution, Quantitative NMR
Abstract: International audience; Introduction Absolute quantification of individual metabolites in complex biological samples is crucial in targeted metabolomic profiling. Objectives An inter-laboratory test was performed to evaluate the impact of the NMR software, peak-area determination method (integration vs. deconvolution) and operator on quantification trueness and precision. Methods A synthetic urine containing 32 compounds was prepared. One site prepared the urine and calibration samples, and performed NMR acquisition. NMR spectra were acquired with two pulse sequences including water suppression used in routine analyses. The pre-processed spectra were sent to the other sites where each operator quantified the metabolites using internal referencing or external calibration, and his/her favourite in-house, open-access or commercial NMR tool. Results For 1D NMR measurements with solvent presaturation during the recovery delay (zgpr), 20 metabolites were successfully quantified by all processing strategies. Some metabolites could not be quantified by some methods. For internal referencing with TSP, only one half of the metabolites were quantified with a trueness below 5%. With peak integration and external calibration, about 90% of the metabolites were quantified with a trueness below 5%. The NMRProcFlow integration module allowed the quantification of several additional metabolites. The number of quantified metabolites and quantification trueness improved for some metabolites with deconvolution tools. Trueness and precision were not significantly different between zgpr- and NOESYpr-based spectra for about 70% of the variables. Conclusion External calibration performed better than TSP internal referencing. Inter-laboratory tests are useful when choosing to better rationalize the choice of quantification tools for NMR-based metabolomic profiling and confirm the value of spectra deconvolution tools.
Published: 2023
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2. Preliminary results of a MetaboHUB 2.0 inter-laboratory test on 1D 1H NMR metabolite quantification of a synthetic urine

Author: Deborde, Catherine, Giraudeau, Patrick, Cahoreau, Edern, da Costa, Gregory, Gautier, Roselyne, Jacob, Daniel, Jousse, Cyril, Lacaze, Melia, Le Mao, Inès, Martineau, Estelle, Moing, Annick, Peyriga, Lindsay, Richard, Tristan, Silvestre, Virginie, Traïkia, Mounir, Canlet, Cécile, Plateforme Bordeaux Metabolome, Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-MetaboHUB-Bordeaux, MetaboHUB-MetaboHUB, Biologie du fruit et pathologie (BFP), Université de Bordeaux (UB)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Chimie Et Interdisciplinarité : Synthèse, Analyse, Modélisation (CEISAM), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Nantes université - UFR des Sciences et des Techniques (Nantes univ - UFR ST), Nantes Université - pôle Sciences et technologie, Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ)-Nantes Université - pôle Sciences et technologie, Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ), Toulouse Biotechnology Institute (TBI), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), MetaToul FluxoMet (TBI-MetaToul), MetaboHUB-MetaToul, MetaboHUB-Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-MetaboHUB-Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Toulouse Biotechnology Institute (TBI), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité de Recherche Œnologie [Villenave d'Ornon] (OENO), Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), ToxAlim (ToxAlim), Université de Toulouse (UT)-Ecole d'Ingénieurs de Purpan (INP - PURPAN), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Metatoul AXIOM (E20 ), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-ToxAlim (ToxAlim), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Ecole d'Ingénieurs de Purpan (INP - PURPAN), Institut de Chimie de Clermont-Ferrand (ICCF), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA)-Institut national polytechnique Clermont Auvergne (INP Clermont Auvergne), Université Clermont Auvergne (UCA)-Université Clermont Auvergne (UCA), Plateforme Exploration du Métabolisme (PFEM), Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-MetaboHUB-Clermont, CAPACITÉS SAS, and Nantes Université (Nantes Univ)
Subjects: [CHIM.ANAL]Chemical Sciences/Analytical chemistry, [SDV]Life Sciences [q-bio]
Abstract: International audience
Published: 2022

3. Defining Benchmark Outcomes for Distal Pancreatectomy: Results of a French Multicentric Study

Author: Thibault Durin, Ugo Marchese, Alain Sauvanet, Safi Dokmak, Zineb Cherkaoui, David Fuks, Christophe Laurent, Marie André, Ahmet Ayav, Cloe Magallon, Olivier Turrini, Laurent Sulpice, Fabien Robin, Philippe Bachellier, Piettro Addeo, François-Régis Souche, Thomas Bardol, Julie Perinel, Mustapha Adham, Stylianos Tzedakis, David Jérémie Birnbaum, Olivier Facy, Johan Gagniere, Sébastien Gaujoux, Ecoline Tribillon, Edouard Roussel, Lilian Schwarz, Louise Barbier, Alexandre Doussot, Nicolas Regenet, Antonio Iannelli, Jean-Marc Regimbeau, Guillaume Piessen, Xavier Lenne, Stéphanie Truant, Mehdi El Amrani, Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC), Service de chirurgie hepato-pancreato-biliaire, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Beaujon [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Cité (UPCité), Hôpital Beaujon [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut de Recherche sur les Maladies Virales et Hépatiques (IVH), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM), CHU Strasbourg, Université Paris Cité (UPCité), Institut des Sciences de la Terre (ISTerre), Institut national des sciences de l'Univers (INSU - CNRS)-Institut de recherche pour le développement [IRD] : UR219-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS)-Université Gustave Eiffel-Université Grenoble Alpes (UGA), Unité de Recherche Œnologie [Villenave d'Ornon] (OENO), Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut des Sciences de la Vigne et du Vin [Villenave d'Ornon] (ISVV), Université de Bordeaux (UB), Moët Hennessy Champagne Services (MHCS), Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy), Faculté de Médecine [Nancy], Université de Lorraine (UL), Aix-Marseille Université - École de médecine (AMU SMPM MED), Aix-Marseille Université - Faculté des sciences médicales et paramédicales (AMU SMPM), Aix Marseille Université (AMU)-Aix Marseille Université (AMU), Centre de Recherche en Cancérologie de Marseille (CRCM), Aix Marseille Université (AMU)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Oncogenesis, Stress, Signaling (OSS), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-CRLCC Eugène Marquis (CRLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM), CHU Pontchaillou [Rennes], Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Département d'Hépato-Gastroentérologie et de Transplantation Hépatique [CHU Saint-Eloi], CHU Saint-Eloi-Université de Montpellier (UM), Centre hépato-biliaire (CHB), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Paul Brousse-Institut National de la Santé et de la Recherche Médicale (INSERM), Hôpital de la Croix-Rousse [CHU - HCL], Hospices Civils de Lyon (HCL), Assistance Publique - Hôpitaux de Marseille (APHM), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Centre d'Investigation Clinique [CHU Clermont-Ferrand] (CIC 1405), Institut National de la Santé et de la Recherche Médicale (INSERM)-Direction de la recherche clinique et de l’innovation [CHU Clermont-Ferrand] (DRCI), CHU Clermont-Ferrand-CHU Clermont-Ferrand, Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte (M2iSH), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre de Recherche en Nutrition Humaine d'Auvergne (CRNH d'Auvergne)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Service de Chirurgie Digestive, Hépato-Bilio-pancréatique et Transplantation Hépatique [CHU Pitié-Salpétrière], CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Hôpital Charles Nicolle [Rouen], Service de chirurgie digestive [CHU Rouen], CHU Rouen, Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU), Génomique et Médecine Personnalisée du Cancer et des Maladies Neuropsychiatriques (GPMCND), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), CHU Trousseau [Tours], Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon), Centre hospitalier universitaire de Nantes (CHU Nantes), Centre méditerranéen de médecine moléculaire (C3M), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Côte d'Azur (UCA), Hôpital l'Archet, Chirurgie digestive [CHU Amiens], CHU Amiens-Picardie, Simplification des soins chez les patients complexes - UR UPJV 7518 (SSPC), Université de Picardie Jules Verne (UPJV), Cancer Heterogeneity, Plasticity and Resistance to Therapies - UMR 9020 - U 1277 (CANTHER), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), CHU Bordeaux [Bordeaux], Université de Rennes (UR)-CRLCC Eugène Marquis (CRLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Hôpital Saint Eloi (CHRU Montpellier), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)-Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)-Université de Montpellier (UM), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), and Normandie Université (NU)-Normandie Université (NU)
Subjects: Surgery, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: International audience; OBJECTIVE: Defining robust and standardized outcome references for distal pancreatectomy (DP) by using Benchmark analysis. BACKGROUND: Outcomes after DP are recorded in medium or small-sized studies without standardized analysis. Therefore, the best results remain uncertain. METHODS: This multicenter study included all patients undergoing DP for resectable benign or malignant tumors in 21 French expert centers in pancreas surgery from 2014 to 2018. A low-risk cohort defined by no significant comorbidities was analyzed to establish 18 outcome benchmarks for DP. These values were tested in high-risk, minimally invasive and benign tumor cohorts. RESULTS: 1188 patients were identified and 749 low-risk patients were screened to establish Benchmark cut-offs. Therefore, Benchmark rate for mini-invasive approach was ≥q36.8%. Benchmark cut-offs for postoperative mortality, major morbidity grade ≥q3a and clinically significant pancreatic fistula rates were 0%, ≤q27% and ≤q28%, respectively. The benchmark rate for readmission was ≤q 16%. For patients with pancreatic adenocarcinoma, cut-offs were ≥q75%, ≥q69.5% and ≥q66% for free resection margins (R0), 1-year disease free-survival and 3-year overall survival, respectively. The rate of mini-invasive approach in high-risk cohort was lower than the Benchmark cut-off (34.1% vs. ≥q36.8%). All Benchmark cut-offs were respected for benign tumor group. The proportion of benchmark cases was correlated to outcomes of DP. Centers with a majority of low-risk patients had worse results than those operating complex cases. CONCLUSION: This large-scale study is the first benchmark analysis of DP outcomes and provides robust and standardized data. This may allow for comparisons between surgeons, centers, studies, and surgical techniques.
Published: 2022
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4. A truncated anti-CRISPR protein prevents spacer acquisition but not interference

Author: Cécile Philippe, Carlee Morency, Pier-Luc Plante, Edwige Zufferey, Rodrigo Achigar, Denise M. Tremblay, Geneviève M. Rousseau, Adeline Goulet, Sylvain Moineau, Département de Biochimie, de Microbiologie et de Bio-informatique, Université Laval, Université Laval [Québec] (ULaval), Groupe de Recherche en Écologie Buccale (GREB), Faculté de Médecine Dentaire, Félix d'Hérelle Reference Center for Bacterial Viruses, Groupe de Recherche en Écologie Buccale (GREB), Unité de Recherche Œnologie [Villenave d'Ornon] (OENO), Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Big Data Research Center, Universidad de la República [Montevideo] (UDELAR), Laboratoire d'ingénierie des systèmes macromoléculaires (LISM), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), and ANR-18-CE11-0016,PHARE,Vengeance de phages: analyses structurales et fonctionnelles de protéines anti CRISPR-Cas9(2018)
Subjects: Multidisciplinary, Bacteria, Virus–host interactions, [SDV]Life Sciences [q-bio], General Physics and Astronomy, Phage biology, Streptococcus thermophilus, Bacteriophages, General Chemistry, CRISPR-Cas Systems, General Biochemistry, Genetics and Molecular Biology
Abstract: CRISPR-Cas systems in prokaryotic cells provide an adaptive immunity against invading nucleic acids. For example, phage infection leads to addition of new immunity (spacer acquisition) and DNA cleavage (interference) in the bacterial model species Streptococcus thermophilus, which primarily relies on Cas9-containing CRISPR-Cas systems. Phages can counteract this defense system through mutations in the targeted protospacers or by encoding anti-CRISPR proteins (ACRs) that block Cas9 interference activity. Here, we show that S. thermophilus can block ACR-containing phages when the CRISPR immunity specifically targets the acr gene. This in turn selects for phage mutants carrying a deletion within the acr gene. Remarkably, a truncated acrIIA allele, found in a wild-type virulent streptococcal phage, does not block the interference activity of Cas9 but still prevents the acquisition of new immunities, thereby providing an example of an ACR specifically inhibiting spacer acquisition.
Published: 2022
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5. The functional microbiome of grapevine throughout plant evolutionary history and lifetime

Author: Fournier, Paola, Pellan, Lucile, Barroso-Bergadà, Didac, Bohan, David, Candresse, Thierry, Delmotte, François, Dufour, Marie-Cécile, Lauvergeat, Virginie, Le Marrec, Claire, Marais, Armelle, Martins, Guilherme, Masneuf-Pomarède, Isabelle, Rey, Patrice, Sherman, David James, This, Patrice, Frioux, Clémence, Labarthe, Simon, Vacher, Corinne, Biodiversité, Gènes & Communautés (BioGeCo), Université de Bordeaux (UB)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Santé et agroécologie du vignoble (UMR SAVE), Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Agroécologie [Dijon], Université de Bourgogne (UB)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Dijon, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Biologie du fruit et pathologie (BFP), Ecophysiologie et Génomique Fonctionnelle de la Vigne (UMR EGFV), Unité de Recherche Œnologie [Villenave d'Ornon] (OENO), Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux (IPREM), Université de Pau et des Pays de l'Adour (UPPA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), from patterns to models in computational biodiversity and biotechnology (PLEIADE), Laboratoire Bordelais de Recherche en Informatique (LaBRI), Université de Bordeaux (UB)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Centre National de la Recherche Scientifique (CNRS)-Université de Bordeaux (UB)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Centre National de la Recherche Scientifique (CNRS)-Inria Bordeaux - Sud-Ouest, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-Biodiversité, Gènes & Communautés (BioGeCo), Université de Bordeaux (UB)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Montpellier (UM), We are very grateful to Maureen Pellan for the design of Fig. 3. The authors acknowledge the support of the French National Research Agency (ANR) under the grants 20-PCPA-0010 (PPR VITAE) and ANR-17-CE32-0011 (NGB), and of FranceAgrimer and CNIV under the grant n°2018-52537 (Vitirhizobiome). LP received funding from Fondation Bordeaux Université, ANR-20-PCPA-0010,VITAE,Cultivating the grapevine without pesticides : towards agroecological wine-producing socio-ecosystems(2020), and ANR-17-CE32-0011,NGB,Biosurveillance Next-Gen des changements dans la structure et le fonctionnement des écosystèmes(2017)
Subjects: Oomycete, Biogeography, Bacteria, [SDV]Life Sciences [q-bio], Plant-microbe interaction, Fungi, Vitis, Metagenomics, Microbial network, Coevolution, Virus
Abstract: International audience; European grapevine is a complex holobiont composed of two plant genomes, that of the scion (Vitis vinifera L.) and the rootstock (Vitis spp.), and a multitude of microbial genomes that collectively form the microbiome. The grapevine microbiome has been extensively described over the last decade, primarily using metabarcoding approaches. Unfortunately, metabarcoding data alone provide little information on microbial functions and outcomes of plant-microbe interactions. Here we review knowledge about the microorganisms that have a demonstrated influence, positive or negative, on the performance of the grapevine holobiont. Our review encompasses bacteria, filamentous fungi, yeasts, oomycetes and viruses. It covers aboveground and belowground microorganisms, including arbuscular mycorrhizal and ectomycorrhizal fungi. We focus on taxa and functions that protect the plant against pathogens and pests, promote growth, increase tolerance to abiotic stresses and highlight those involved in disease and decline. As the outcomes of plant-microbe interactions are labile, we examine the dynamics and functions of grapevine-microbiome interactions over both the plant lifetime and the plant evolutionary history, beginning with plant domestication. Based on the knowledge and gaps we identify, we suggest field sampling designs, culture-based experiments, molecular tools and theoretical analysis methods, including shotgun metagenomics and network models, that could be used in future research to uncover and leverage the full functional potential of the grapevine microbiome.
Published: 2022
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6. Is deficit irrigation with saline waters a viable alternative for winegrowers in semiarid areas?

Author: Alejandro Martínez-Moreno, Eva Pérez-Álvarez, Ramon López-Urrea, Diego Intrigliolo, M. Reyes González-Centeno, Pierre-Louis Teissedre, Rocío Gil-Muñoz, Universidad de Murcia, Centro de Edafologia y Biologia aplicada del Segura (CEBAS - CSIC), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Unité de Recherche Œnologie [Villenave d'Ornon] (OENO), Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Instituto Murciano de Investigación y desarrollo Agrario y Alimentario (IMIDA), European Project: CTM2016-78607-P ,AEI/FEDER, Ministerio de Ciencia, Innovación y Universidades (España), and Agencia Estatal de Investigación (España)
Subjects: Monastrell, Botany, [SDV.SA.AGRO]Life Sciences [q-bio]/Agricultural sciences/Agronomy, food and beverages, Agriculture, phenolic compounds, Horticulture, anthocyanins, salinity, QK1-989, Vitis vinifera, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Food Science
Abstract: Two of the main challenges of Mediterranean viticulture is low water quality and the risk of increasing concentrations of mineral salts in the root zone. This work was undertaken to study the impact of saline deficit irrigation on grape and wine phenolic composition, as well as on the sensory profile of the wines. The experiment was carried out during three consecutive years (2016-2018) in a commercial vineyard of cv. Monastrell (Vitis vinifera L.) grafted onto 1103P rootstock located in D.O. Jumilla (SE Spain). Three watering regimes were carried out: i) the control (“Control”): the vines were irrigated with water of standard quality, ii) Sulfate treatment (“Sul”): the vines were irrigated with saline water (Na₂SO₄ + MgSO4), and iii) Chloride treatment (“Chl”): the vines were irrigated with saline water (NaCl). The same amount of irrigation water was applied to all the treatments. The water electrical conductivity was 1.8 dS/m for Control and 5 dS/m for the saline treatments (Sul and Chl). Both the Sul and Chl treatments reduced the berry weight in all the study years compared to Control, although this difference was statistically significant in the last year only (p ≤ 0.05). No significant differences (p > 0.05) were observed in the grape quality parameters. However, the saline treatments slightly increased grape total soluble solids (TSS) in two out of the three study years compared to Control. Regarding the phenolic composition, no significant differences (p > 0.05) among treatments were found in grapes and wines. In general, the wines from vines irrigated with saline waters received the best scores by the panel in the sensory profile analysis. The use of saline waters could be employed in the case of water scarcity, as long as the vineyard is planted on a rootstock tolerant to salinity, such as 1103P, and the vineyard soil has a texture that favours leaching., The research was funded by AEI-FEDER project AGL2017-83738-C3-3-R.
Published: 2022
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7. CODEVI-SDP -Mesurer et caractériser les Composés Organiques Volatils de la vigne comme réponse à une application d'un produit Stimulateur des Défenses des Plantes

Author: Aveline, Nicolas, Lemaitre-Guillier, Christelle, Douillet, A., Cluzet, Stéphanie, Fonayet, Josep Valls, Dufresne, C., Chartier, Agnes, Adrian, M., Daire, Xavier, Institut Français de la Vigne et du Vin (IFV), Santé des Écosystèmes Viticoles Économes en intraNts [Villenave d'Ornon] (UMT SEVEN), Institut Français de la Vigne et du Vin (IFV)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Agroécologie [Dijon], Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité de Recherche Œnologie [Villenave d'Ornon] (OENO), Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut de Chimie Organique et Analytique (ICOA), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Composés Organiques Volatils, Plasmopara viticola, SDP, Volatile Organic Compound, [SDE]Environmental Sciences, biocontrôle, viticulture, Plant Defence Inducer, grapevine
Abstract: Ce volume regroupe les textes issus du programme Casdar "Innovation et Partenariat" et "Recherche technologique" de 2016. Il a été réalisé sous l’égide du GIS Relance Agronomique. Ce volume regroupe les textes issus du programme Casdar "Innovation et Partenariat" et "Recherche technologique" de 2016. Il a été réalisé sous l’égide du GIS Relance Agronomique. Ce volume regroupe les textes issus du programme Casdar "Innovation et Partenariat" et "Recherche technologique" de 2016. Il a été réalisé sous l’égide du GIS Relance Agronomique.; Volatile organic compounds (VOCs) are molecules emitted into the air by the plant during a stress, as the attack of a pest. The objective of the CODEVI SDP project (2017-2019) was to characterize the VOCs emitted by the vine after being treated with registered Plant Defence Inducers (PDI) and to inform whether there is a correlation between this emission and the resistance induced against downy mildew (P. viticola). This emission measurement could be an index to validate a plant response to the action of a PDI application and to improve their practical use at the vineyard. The project was conducted in Burgundy and Bordeaux vineyards and joined research and technical teams (UMR AE, IFV and University of Bordeaux) of these territories for the experimentations. The expertise in the measurement and analysis of VOCs was provided by the ICOA laboratory (CNRS Orléans). The main part of the measurements was carried out based on the theoretical triptych that should follow the application of the PDI: emission of VOCs by the vine / expression of defence molecules in the leaves / efficiency in control of P. viticola. In controlled conditions, using leaf cuttings under confined atmosphere, UMR AE showed the performance of Twister® sensors, and that some specific VOCs were emitted after application of PDIs (including the registered product Bastid®), notably the sesquiterpenes beta-caryophyllene and alpha-farnesene and an increase of stilbenes in leaves. Nevertheless, straight correlation between those production and protection against downy mildew is not obvious and rather suggests combined actions of numerous molecules to build an efficient defence. In the vineyard, on microplots of vines, the results are less clear and the signals weaker. The first VOCs capture methodology that was proposed was not indicative in a such open atmosphere and in presence of numerous uncontrollable parameters. Thus, it was modified with bagged branches to concentrate the VOCs response on the sensors. Moreover, the PDI Bastid® showed only poor levels of protection against downy mildew in the pathotests performed on leaf discs and no control in the vineyard. From the results harvested during the three years, under three extremely different environmental conditions, the initial triptych was not established in this part of the project. Nevertheless, some global profiles could be highlighted, with noticeable terpene amounts of beta-ocimene and beta-caryophyllene within the scent of the elicited vineyard.; Les composés organiques volatiles (COV) sont des molécules émises dans l’air par la plante, notamment lors d’un stress (attaque d’un bioagresseur). Le projet CODEVI SDP avait pour objectif de caractériser les COV émis par la vigne lors de son traitement avec des produits Stimulateurs des défenses homologuées et de renseigner l’existence potentielle d’une corrélation entre cette émission et la résistance induite contre le mildiou (Plasmopara viticola). Cette émission de COV pourrait devenir un indice de réaction de la plante permettant de valider au vignoble l’action d’une application de produit Stimulateurs des défenses des plantes (SDP) et permettre une optimisation de leur usage en conditions de production. Le projet a été mené sur des vignobles Bourguignon et Bordelais et a rassemblé des équipes de recherche implantées dans ces territoires (UMR AE Dijon, IFV et Université de Bordeaux) pour la réalisation des expérimentations sous l’expertise du laboratoire de l’ICOA (UMR 7311 Orléans) pour la mesure et l’analyse des COV. Suite à l’application de produits SDP, l’essentiel des mesures a été mené sur la base du triptyque théorique : émission de COV par la vigne, synthèse de molécules de défense dans les feuilles et efficacité de protection contre P. viticola. En conditions contrôlées, surboutures foliaires sous atmosphère confinée, l’UMR AE a montré l’intérêt de l’utilisation des capteurs Twister® et que quelques COV spécifiques étaient émis après application de certains SDP (dont le produit homologué Bastid®), notamment des sesquiterpènes dont le bêta-caryophyllène et l’alpha-farnésène, ainsi qu’une augmentation des stilbènes dans les feuilles. Au vignoble, sur des microplacettes de ceps en production, les signaux sont plus faibles et les résultats en découlant plus difficiles à interpréter. La première méthodologie de capture des COV qui a été entreprise n’a pas été idéale face à une atmosphère ouverte et de nombreux paramètres non maîtrisables, celle-ci a ensuite été modifiée en utilisant l’ensachage des rameaux pour concentrer l’adsorption des COV sur les capteurs. De plus, le produit SDP Bastid® n’a induit que de faibles niveaux de protection contre le mildiou dans les pathotests sur disques de feuilles et pas de protection au vignoble. A partir des résultats cumulés sur trois années successives, présentant trois conditions climatiques extrêmement différentes, le triptyque initial n’a pas été établi pour cette partie terrain. Cependant, des profils généraux d’émission de COV peuvent être mis en évidence, avec une proportion non négligeable de terpènes bêta-ocimène et de bêta-caryophyllène dans les « bouquets » émis par des parcelles de vigne élicitées.
Published: 2022
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8. Secondary Metabolism Rearrangements in Linum usitatissimum L. after Biostimulation of Roots with COS Oligosaccharides from Fungal Cell Wall

Author: Redouan Elboutachfaiti, Roland Molinié, David Mathiron, Yannis Maillot, Jean-Xavier Fontaine, Serge Pilard, Anthony Quéro, Clément Brasselet, Marguerite Dols-Lafargue, Cédric Delattre, Emmanuel Petit, Biologie des Plantes et Innovation - UR UPJV 3900 (BIOPI), Université de Picardie Jules Verne (UPJV)-Transfrontalière BioEcoAgro - UMR 1158 (BioEcoAgro), Université d'Artois (UA)-Université de Liège-Université de Picardie Jules Verne (UPJV)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Université d'Artois (UA)-Université de Liège-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), Transfrontalière BioEcoAgro - UMR 1158 (BioEcoAgro), Plateforme Analytique (PFA), Université de Picardie Jules Verne (UPJV), Institut national polytechnique Clermont Auvergne (INP Clermont Auvergne), Université Clermont Auvergne (UCA), Unité de Recherche Œnologie [Villenave d'Ornon] (OENO), Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut Pascal (IP), Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA)-Institut national polytechnique Clermont Auvergne (INP Clermont Auvergne), Université Clermont Auvergne (UCA)-Université Clermont Auvergne (UCA), Institut Universitaire de France (IUF), and Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.)
Subjects: roots elicitation, secondary metabolites, [SDV]Life Sciences [q-bio], Organic Chemistry, seedlings, Pharmaceutical Science, food and beverages, Linum usitatissimum L, Analytical Chemistry, Chemistry (miscellaneous), Drug Discovery, Molecular Medicine, COS oligosaccharides, Physical and Theoretical Chemistry
Abstract: International audience; In vitro culture of flax (Linum usitatissimum L.) was exposed to chitosan oligosaccharides (COS) in order to investigate the effects on the growth and secondary metabolites content in roots and shoots. COS are fragments of chitosan released from the fungal cell wall during plant-pathogen interactions. They can be perceived by the plant as pathogen-associated signals, mediating local and systemic innate immune responses. In the present study, we report a novel COS oligosaccharide fraction with a degree of polymerization (DP) range of 2-10, which was produced from fungal chitosan by a thermal degradation method and purified by an alcohol-precipitation process. COS was dissolved in hydroponic medium at two different concentrations (250 and 500 mg/L) and applied to the roots of growing flax seedlings. Our observations indicated that the growth of roots and shoots decreased markedly in COS-treated flax seedlings compared to the control. In addition, the results of a metabolomics analysis showed that COS treatment induced the accumulation of (neo)lignans locally at roots, flavones luteolin C-glycosides, and chlorogenic acid in systemic responses in the shoots of flax seedlings. These phenolic compounds have been previously reported to exhibit a strong antioxidant and antimicrobial activities. COS oligosaccharides, under the conditions applied in this study (high dose treatment with a much longer exposure time), can be used to indirectly trigger metabolic response modifications in planta, especially secondary metabolism, because during fungal pathogen attack, COS oligosaccharides are among the signals exchanged between the pathogen and host plant.
Published: 2022
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9. Chiroptical and Potential In Vitro Anti-Inflammatory Properties of Viniferin Stereoisomers from Grapevine (Vitis vinifera L)

Author: Guillaume Buffeteau, Ruth Hornedo-Ortega, Julien Gabaston, Nicolas Daugey, Antonio Palos-Pinto, Anne Thienpont, Thierry Brotin, Jean-Michel Mérillon, Thierry Buffeteau, Pierre Waffo-Teguo, Unité de Recherche Œnologie [Villenave d'Ornon] (OENO), Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut des Sciences Moléculaires (ISM), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1 (UB)-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Chimie - UMR5182 (LC), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Anti-Inflammatory Agents, chirality, Stereoisomerism, Wine, General Medicine, [CHIM.THER]Chemical Sciences/Medicinal Chemistry, Analytical Chemistry, stilbene, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Stilbenes, stereoisomers, Vitis, vine, viniferins, Food Science, anti-inflammatory
Abstract: International audience; Determination of stereochemistry and enantiomeric excess in chiral natural molecules is a research of great interest because enantiomers can exhibit different biological activities. Viniferin stilbene dimers are natural molecules present in grape berries and wine but also, in larger amount, in stalks of grapevine. Four stereoisomers of viniferin stilbene dimers (7aS,8aS)-E-ε-viniferin (1a), (7aR,8aR)-E-ε-viniferin (1b), (7aS,8aR)-Eε-viniferin (2a), and (7aR,8aS)-E-ω-viniferin (2b) were isolated from grapevine stalks of Cabernet Sauvignon, Merlot and Sauvignon Blanc, using a combination of centrifugal partition chromatography (CPC), preparative and chiral HPLC. The structure elucidation of these molecules was achieved by NMR whereas the absolute configurations of the four stereoisomers were investigated by vibrational circular dichroism spectroscopy in combination with density functional theory (DFT) calculations. This study unambiguously established the (+)-(7aS,8aS) and (+)-(7aR,8aS) configurations for E-ε-viniferin and E-ω-viniferin, respectively. Finally, we show that Cabernet Sauvignon provided the quasi enantiopure (+)-(7aS,8aS)-E-εviniferin compound which presents the best anti-inflammatory and anti-oxidant activities.
Published: 2022
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10. CODEVI-SDP - Quantification and identification of grapevine volatile organic compounds as an indicator of defense response after an application of Plant Defence Inducer

Author: Aveline, Nicolas, Lemaitre-Guillier, Christelle, Douillet, A., Cluzet, Stéphanie, Fonayet, Josep Valls, Dufresne, C., Chartier, Agnes, Adrian, M., Daire, Xavier, Institut Français de la Vigne et du Vin (IFV), Santé des Écosystèmes Viticoles Économes en intraNts [Villenave d'Ornon] (UMT SEVEN), Institut Français de la Vigne et du Vin (IFV)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Agroécologie [Dijon], Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité de Recherche Œnologie [Villenave d'Ornon] (OENO), Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut de Chimie Organique et Analytique (ICOA), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and DESAILLY, Marion
Subjects: Composés Organiques Volatils, [SDE] Environmental Sciences, Plasmopara viticola, SDP, Volatile Organic Compound, [SDE]Environmental Sciences, biocontrôle, viticulture, Plant Defence Inducer, grapevine
Abstract: Volatile organic compounds (VOCs) are molecules emitted into the air by the plant during a stress, as the attack of a pest. The objective of the CODEVI SDP project (2017-2019) was to characterize the VOCs emitted by the vine after being treated with registered Plant Defence Inducers (PDI) and to inform whether there is a correlation between this emission and the resistance induced against downy mildew (P. viticola). This emission measurement could be an index to validate a plant response to the action of a PDI application and to improve their practical use at the vineyard. The project was conducted in Burgundy and Bordeaux vineyards and joined research and technical teams (UMR AE, IFV and University of Bordeaux) of these territories for the experimentations. The expertise in the measurement and analysis of VOCs was provided by the ICOA laboratory (CNRS Orléans). The main part of the measurements was carried out based on the theoretical triptych that should follow the application of the PDI: emission of VOCs by the vine / expression of defence molecules in the leaves / efficiency in control of P. viticola. In controlled conditions, using leaf cuttings under confined atmosphere, UMR AE showed the performance of Twister® sensors, and that some specific VOCs were emitted after application of PDIs (including the registered product Bastid®), notably the sesquiterpenes beta-caryophyllene and alpha-farnesene and an increase of stilbenes in leaves. Nevertheless, straight correlation between those production and protection against downy mildew is not obvious and rather suggests combined actions of numerous molecules to build an efficient defence. In the vineyard, on microplots of vines, the results are less clear and the signals weaker. The first VOCs capture methodology that was proposed was not indicative in a such open atmosphere and in presence of numerous uncontrollable parameters. Thus, it was modified with bagged branches to concentrate the VOCs response on the sensors. Moreover, the PDI Bastid® showed only poor levels of protection against downy mildew in the pathotests performed on leaf discs and no control in the vineyard. From the results harvested during the three years, under three extremely different environmental conditions, the initial triptych was not established in this part of the project. Nevertheless, some global profiles could be highlighted, with noticeable terpene amounts of beta-ocimene and beta-caryophyllene within the scent of the elicited vineyard., Les composés organiques volatiles (COV) sont des molécules émises dans l’air par la plante, notamment lors d’un stress (attaque d’un bioagresseur). Le projet CODEVI SDP avait pour objectif de caractériser les COV émis par la vigne lors de son traitement avec des produits Stimulateurs des défenses homologuées et de renseigner l’existence potentielle d’une corrélation entre cette émission et la résistance induite contre le mildiou (Plasmopara viticola). Cette émission de COV pourrait devenir un indice de réaction de la plante permettant de valider au vignoble l’action d’une application de produit Stimulateurs des défenses des plantes (SDP) et permettre une optimisation de leur usage en conditions de production. Le projet a été mené sur des vignobles Bourguignon et Bordelais et a rassemblé des équipes de recherche implantées dans ces territoires (UMR AE Dijon, IFV et Université de Bordeaux) pour la réalisation des expérimentations sous l’expertise du laboratoire de l’ICOA (UMR 7311 Orléans) pour la mesure et l’analyse des COV. Suite à l’application de produits SDP, l’essentiel des mesures a été mené sur la base du triptyque théorique : émission de COV par la vigne, synthèse de molécules de défense dans les feuilles et efficacité de protection contre P. viticola. En conditions contrôlées, surboutures foliaires sous atmosphère confinée, l’UMR AE a montré l’intérêt de l’utilisation des capteurs Twister® et que quelques COV spécifiques étaient émis après application de certains SDP (dont le produit homologué Bastid®), notamment des sesquiterpènes dont le bêta-caryophyllène et l’alpha-farnésène, ainsi qu’une augmentation des stilbènes dans les feuilles. Au vignoble, sur des microplacettes de ceps en production, les signaux sont plus faibles et les résultats en découlant plus difficiles à interpréter. La première méthodologie de capture des COV qui a été entreprise n’a pas été idéale face à une atmosphère ouverte et de nombreux paramètres non maîtrisables, celle-ci a ensuite été modifiée en utilisant l’ensachage des rameaux pour concentrer l’adsorption des COV sur les capteurs. De plus, le produit SDP Bastid® n’a induit que de faibles niveaux de protection contre le mildiou dans les pathotests sur disques de feuilles et pas de protection au vignoble. A partir des résultats cumulés sur trois années successives, présentant trois conditions climatiques extrêmement différentes, le triptyque initial n’a pas été établi pour cette partie terrain. Cependant, des profils généraux d’émission de COV peuvent être mis en évidence, avec une proportion non négligeable de terpènes bêta-ocimène et de bêta-caryophyllène dans les « bouquets » émis par des parcelles de vigne élicitées.
Published: 2022
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11. Elicitor-Induced VOC Emission by Grapevine Leaves: Characterisation in the Vineyard

Author: Christelle Lemaitre-Guillier, Agnès Chartier, Christelle Dufresne, Antonin Douillet, Stéphanie Cluzet, Josep Valls, Nicolas Aveline, Xavier Daire, Marielle Adrian, Chartier, Agnes, Agroécologie [Dijon], Université de Bourgogne (UB)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Dijon, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut de Chimie Organique et Analytique (ICOA), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut Français de la Vigne et du Vin (IFV), Unité de Recherche Œnologie [Villenave d'Ornon] (OENO), Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and This work was supported by the French Ministery of Agriculture CASDAR, ConseilRégional Bourgogne Franche-Comté, Bordeaux Metabolome Facility and MetaboHub: CASDARproject CODEVI-SDP (2017-2020)
Subjects: Volatile Organic Compounds, volatile organic compounds (VOCs), Copper Sulfate, Farms, elicitor, Terpenes, Organic Chemistry, Pharmaceutical Science, Cyclopentanes, bar sorptive extraction (SBSE), Acetates, vineyard, grapevine, stir bar sorptive extraction (SBSE), Analytical Chemistry, Plant Leaves, Chemistry (miscellaneous), Stilbenes, Drug Discovery, [CHIM] Chemical Sciences, Molecular Medicine, [CHIM]Chemical Sciences, Oxylipins, Physical and Theoretical Chemistry, stir
Abstract: International audience; The present study is aimed at determining whether leaf volatile organic compounds (VOCs) are good markers of the grapevine response to defence elicitors in the field. It was carried out in two distinct French vineyards (Burgundy and Bordeaux) over 3 years. The commercial elicitor Bastid® (Syngenta, Saint-Sauveur, France) (COS-OGA) was first used to optimise the VOCs’ capture in the field; by bagging stems together with a stir bar sorptive extraction (SBSE) sensor. Three elicitors (Bastid®, copper sulphate and methyl jasmonate) were assessed at three phenological stages of the grapevines by monitoring stilbene phytoalexins and VOCs. Stilbene production was low and variable between treatments and phenological stages. VOCs—particularly terpenes—were induced by all elicitors. However, the response profiles depended on the type of elicitor, the phenological stage and the vineyard, and no sole common VOC was found. The levels of VOC emissions discriminated between weak (Bastid® and copper sulphate) and strong (methyl jasmonate) inducers. Ocimene isomers were constitutively present in the overall blends of the vineyards and increased by the elicitors’ treatments, whilst other VOCs were newly released throughout the growing seasons. Nonetheless, the plant development and climate factors undoubtedly influenced the release and profiles of the leaf VOCs.
Published: 2022
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12. Differential Uridyl-diphosphate-Glucuronosyl Transferase 1A enzymatic arsenal explains the specific cytotoxicity of resveratrol towards tumor colorectal cells

Author: Samuel Amintas, Pauline Beaumont, Charles Dupin, Isabelle Moranvillier, Isabelle Lamrissi, Elie Patel, Benjamin Fernandez, Alice Bibeyran, Julian Boutin, Tristan Richard, Stéphanie Krisa, François Moreau-Gaudry, Aurélie Bedel, David Cappellen, Benoît Pinson, Véronique Vendrely, Sandrine Dabernat, BoRdeaux Institute in onCology (Inserm U1312 - BRIC), Université de Bordeaux (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM), CHU Bordeaux [Bordeaux], Unité de Recherche Œnologie [Villenave d'Ornon] (OENO), Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), TBM-Core [Bordeaux] (CNRS UMS 3427 - INSERM US 005), Université de Bordeaux (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de biochimie et génétique cellulaires (IBGC), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), and Admin, Oskar
Subjects: Nutrition and Dietetics, Bioactive food components, Medicine (miscellaneous), [SDV.CAN]Life Sciences [q-bio]/Cancer, [SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, Colorectal cancer, Nucleotide metabolism, [SDV.MHEP.HEG] Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, [SDV.CAN] Life Sciences [q-bio]/Cancer, Tumorigenesis, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, UGT, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, Drug sensitivity, Food Science
Abstract: Resveratrol belongs to the Bioactive Food Component (BFC) family. It seems admitted that its cytotoxic action impacts tumor cells and spares healthy cells, but the published proofs remain rare. We hypothesized that cells may differentially metabolize resveratrol and lead to different systemic impacts. For this, resveratrol metabolization was evaluated by ultra-high-performance liquid chromatography (UHPLC) coupled with diode array detection (DAD), and correlated with the expression of Uridyl-diphosphate-Glucuronosyl Transferase 1A (UGT1A) genes. The expression of UGT1A genes in human colorectal tissues was studied with RNAseq databases. Functional validation of UGT1A enzymes implication in resveratrol sensitivity of colorectal cells established by UGT1A expression modulation. As resveratrol impacts the S phase of the cell cycle, nucleotide metabolic balance was assessed. We found that resveratrol was more cytotoxic in cells with downregulation of UGTs, i.e. tumor cells. Conversely, overexpression of the UGT1A10 gene in an initial resveratrol-sensitive tumor cell line restored the metabolization accompanied by cytotoxicity diminution. Resveratrol affected intestinal sensitive tumor cell homeostasis with a cell growth/proliferation decoupling, cell-cycle modulation, and UXP/AXP nucleotide imbalance resulting in a global reduction of transcription and translation. This impact on global cell activity was restricted to tumor cells. This study improves resveratrol’s general knowledge and explains how its antitumor action can spare non-tumor cells. It also paves the way to select colorectal tumors eligible for resveratrol treatment potentiation without additional toxicity to healthy digestive tissues.
Published: 2022
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13. Different trajectories of polyploidization shape the genomic landscape of the Brettanomyces bruxellensis yeast species

Author: Anne Friedrich, Chris Eberlein, Warren Albertin, Omar Abou Saada, Joseph Schacherer, Génétique moléculaire, génomique, microbiologie (GMGM), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Unité de Recherche Oenologie [Villenave d'Ornon] (OENO), Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), ANR-18-CE12-0013,RecombFun,Evolution du patron de recombinaison et de mutations fonctionnelles à travers différentes espèces(2018), ANR-10-IDEX-0002,UNISTRA,Par-delà les frontières, l'Université de Strasbourg(2010), ANR-20-SFRI-0012,STRAT'US,Façonner les talents en formation et en recherche à l'Université de Strasbourg(2020), ANR-17-EURE-0023,IMCBio,Integrative Molecular and Cellular Biology(2017), ANR-18-CE20-0003,BrettAdapt,Approche multi-échelle de l'adaptation de la levure Brettanomyces bruxellensis aux procédés fermentaires(2018), and European Project: 772505,PhenomeNal
Subjects: 0106 biological sciences, 0303 health sciences, biology, Brettanomyces, Research, fungi, Brettanomyces bruxellensis, food and beverages, pathological conditions, signs and symptoms, biology.organism_classification, 01 natural sciences, Genome, Yeast, Genetic divergence, Loss of heterozygosity, 03 medical and health sciences, Polyploid, Evolutionary biology, Genetic algorithm, [SDE]Environmental Sciences, Genetics, Genetics (clinical), 030304 developmental biology, 010606 plant biology & botany
Abstract: Polyploidization events are observed across the tree of life and occur in many fungi, plant, and animal species. During evolution, polyploidy is thought to be an important source of speciation and tumorigenesis. However, the origin of polyploid populations is not always clear, and little is known about the precise nature and structure of their complex genome. Using a long-read sequencing strategy, we sequenced 71 strains from the Brettanomyces bruxellensis yeast species, which is found in anthropized environments (e.g., beer, contaminant of wine, kombucha, and ethanol production) and characterized by several polyploid subpopulations. To reconstruct the polyploid genomes, we phased them by using different strategies and found that each subpopulation had a unique polyploidization history with distinct trajectories. The polyploid genomes contain either genetically closely related (with a genetic divergence 3%), indicating auto- as well as allopolyploidization events. These latest events have occurred independently with a specific and unique donor in each of the polyploid subpopulations and exclude the known Brettanomyces sister species as possible donors. Finally, loss of heterozygosity events has shaped the structure of these polyploid genomes and underline their dynamics. Overall, our study highlights the multiplicity of the trajectories leading to polyploid genomes within the same species.
Published: 2021
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14. VOCs Are Relevant Biomarkers of Elicitor-Induced Defences in Grapevine

Author: Lucile Jacquens, Christelle Dufresne, Josep Valls, Nicolas Aveline, Stéphanie Cluzet, Marielle Adrian, Xavier Daire, Christelle Lemaître-Guillier, Antonin Douillet, Agnes Chartier, Agroécologie [Dijon], Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut de Chimie Organique et Analytique (ICOA), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Unité de Recherche Oenologie [Villenave d'Ornon] (OENO), Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut Français de la Vigne et du Vin (IFV), ANR-11-INBS-0010,METABOHUB,Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation(2011), DESAILLY, Marion, Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation - - METABOHUB2011 - ANR-11-INBS-0010 - INBS - VALID, Université d'Orléans (UO)-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut de Chimie du CNRS (INC)-Institut National de la Santé et de la Recherche Médicale (INSERM), and Unité de Recherche Oenologie [Villenave d'Ornon]
Subjects: 0106 biological sciences, 0301 basic medicine, [SDE] Environmental Sciences, Farnesene, plant defence, Pharmaceutical Science, Organic chemistry, Sesquiterpene, 01 natural sciences, Gas Chromatography-Mass Spectrometry, Article, Analytical Chemistry, Terpene, 03 medical and health sciences, chemistry.chemical_compound, Laminarin, QD241-441, volatile organic compounds, Drug Discovery, Vitis, Physical and Theoretical Chemistry, Plant Diseases, elicitor, food and beverages, Defence response, Fungicides, Industrial, Elicitor, grapevine, Plant Leaves, Fungicide, Horticulture, 030104 developmental biology, chemistry, Chemistry (miscellaneous), [SDE]Environmental Sciences, stir bar sorptive extraction SBSE-GC-MS, Molecular Medicine, Downy mildew, Sesquiterpenes, terpenes, 010606 plant biology & botany
Abstract: Grapevine is susceptible to fungal diseases generally controlled by numerous chemical fungicides. Elicitors of plant defence are a way of reducing the use of these chemicals, but still provide inconsistent efficiency. Easy-to-analyse markers of grapevine responses to elicitors are needed to determine the best conditions for their efficiency and position them in protection strategies. We previously reported that the elicitor sulphated laminarin induced the emission of volatile organic compounds (VOCs) by grapevine leaves. The present study was conducted to characterise and compare VOC emissions in response to other elicitors. Bastid® was first used to test the conditions of VOC collection and analysis. Using SBSE-GC-MS, we detected several VOCs, including the sesquiterpene α-farnesene, in a time-dependent manner. This was correlated with the induction of farnesene synthase gene expression, in parallel with stilbene synthesis (another defence response), and associated to resistance against downy mildew. The other elicitors (Redeli®, Romeo®, Bion®, chitosan, and an oligogalacturonide) induced VOC emission, but with qualitative and quantitative differences. VOC emission thus constitutes a response of grapevine to elicitors of various chemical structures. Therefore, VOC analysis is relevant for studying the impact of environmental factors on grapevine defence responses and optimising the performance of elicitors in vineyards.
Published: 2021
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15. Investigation of lipase-ligand interactions in porcine pancreatic extracts by microscale thermophoresis

Author: Axel Marchal, Syntia Fayad, Cyril Colas, Rouba Nasreddine, Ghassan Al Hamoui Dit Banni, Reine Nehmé, Institut de Chimie Organique et Analytique (ICOA), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut de chimie et procédés pour l'énergie, l'environnement et la santé (ICPEES), Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Matériaux et Nanosciences Grand-Est (MNGE), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Unité de Recherche Oenologie [Villenave d'Ornon] (OENO), Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université d'Orléans (UO)-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut de Chimie du CNRS (INC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Strasbourg (UNISTRA)-Matériaux et nanosciences d'Alsace (FMNGE), Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), and Unité de Recherche Oenologie [Villenave d'Ornon]
Subjects: Swine, Pancreatic Extracts, 02 engineering and technology, Ligands, 01 natural sciences, Biochemistry, Analytical Chemistry, Small Molecule Libraries, Hydrolysis, Capillary electrophoresis, Animals, Lipase, Ammonium sulfate precipitation, Chromatography, biology, Chemistry, Microscale thermophoresis, 010401 analytical chemistry, 021001 nanoscience & nanotechnology, Ligand (biochemistry), 0104 chemical sciences, Dissociation constant, [SDE]Environmental Sciences, biology.protein, 0210 nano-technology, Protein Binding
Abstract: International audience; The evaluation of binding affinities between large biomolecules and small ligands is challenging and requires highly sensitive techniques. Microscale thermophoresis (MST) is an emerging biophysical technique used to overcome this limitation. This work describes the first MST binding method to evaluate binding affinities of small ligands to lipases from crude porcine pancreatic extracts. The conditions of the MST assay were thoroughly optimized to successfully evaluate the dissociation constant (Kd) between pancreatic lipases (PL) and triterpenoid compounds purified from oakwood. More precisely, the fluorescent labeling of PL (PL*) using RED-NHS dye was achieved via a buffer exchange procedure. The MST buffer was composed of 20 mM NaH2PO4 + 77 mM NaCl (pH 6.6) with 0.05% Triton-X added to efficiently prevent protein aggregation and adsorption, even when using only standard, uncoated MST capillaries. Storage at −20 °C ensured stability of PL* and its fluorescent signal. MST results showed that crude pancreatic extracts were suitable as a source of PL for the evaluation of binding affinities of small ligands. Quercotriterpenoside-I (QTT-I) demonstrated high PL* binding affinity (31 nM) followed by 3-O-galloylbarrinic acid (3-GBA) (500 nM) and bartogenic acid (BA) (1327 nM). To enrich the 50 kDa lipase responsible for the majority of hydrolysis activity in the crude pancreatic extracts, ammonium sulfate precipitation was attempted and its efficiency confirmed using capillary electrophoresis (CE)-based activity assays and HRMS. Moreover, to accurately explain enzyme modulation mechanism, it is imperative to complement binding assays with catalytic activity ones.
Published: 2021
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16. Screening for pancreatic lipase natural modulators by capillary electrophoresis hyphenated to spectrophotometric and conductometric dual detection

Author: Reine Nehmé, Rouba Nasreddine, Axel Marchal, Syntia Fayad, Jean-Christophe Rossi, Hervé Cottet, Ghassan Al Hamoui Dit Banni, Laurent Leclercq, Phu Cao-Ngoc, Leclercq, Laurent, Institut de Chimie Organique et Analytique (ICOA), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Unité de Recherche Œnologie [Villenave d'Ornon] (OENO), Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), Université de Montpellier (UM), Université d'Orléans (UO)-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut de Chimie du CNRS (INC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de chimie et procédés pour l'énergie, l'environnement et la santé (ICPEES), Université de Strasbourg (UNISTRA)-Matériaux et nanosciences d'Alsace (FMNGE), Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Unité de Recherche Oenologie [Villenave d'Ornon], Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), ANR-11-LABX-0029,SYNORG,Synthèse Organique : des molécules au vivant(2011), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Université d'Orléans (UO)-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Matériaux et nanosciences d'Alsace, and Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Université de Strasbourg (UNISTRA)
Subjects: Tris, [CHIM.ANAL] Chemical Sciences/Analytical chemistry, Capillary electrophoresis lipase assay, UPLC/MS molecular characterization of water plant extracts, [CHIM.THER] Chemical Sciences/Medicinal Chemistry, education, TDLFP based on-line enzymatic assay, 02 engineering and technology, [CHIM.THER]Chemical Sciences/Medicinal Chemistry, 01 natural sciences, Biochemistry, Analytical Chemistry, Enzyme catalysis, Hydrolysis, chemistry.chemical_compound, Capillary electrophoresis, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Contactless conductivity detector, Electrochemistry, Environmental Chemistry, [CHIM]Chemical Sciences, Lipase, Spectroscopy, health care economics and organizations, Chromatography, biology, Chemistry, 010401 analytical chemistry, Natural inhibitor screening, Electrophoresis, Capillary, Substrate (chemistry), 021001 nanoscience & nanotechnology, 0104 chemical sciences, MOPS, Kinetics, [CHIM.POLY]Chemical Sciences/Polymers, Spectrophotometry, Dipalmitoylphosphatidylcholine, [SDE]Environmental Sciences, biology.protein, 0210 nano-technology
Abstract: International audience; The search for novel pancreatic lipase (PL) inhibitors has gained increasing attention in recent years. For the first time, a dual detection capillary electrophoresis (CE)-based homogeneous lipase assay was developed employing both the offline and online reaction modes. The hydrolysis of 4-nitrophenyl butyrate (4-NPB) catalyzed by PL into 4-nitrophenol and butyrate was monitored by spectrophotometric and conductimetric detection, respectively. The assays presented several advantages such as economy in consumption (few tens of nanoliters for online assays to few tens of microliters for offline assays), no modification of lipase, rapidity (
Published: 2021
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17. La parole citoyenne face aux enjeux agroalimentaires et environnementaux : étude sémiotique des États généraux de l'alimentation

Author: CHOLET, Céline, Unité de Recherche Oenologie [Villenave d'Ornon], Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Coutant, Karine, and Unité de Recherche Oenologie [Villenave d'Ornon] (OENO)
Subjects: valeurs, semiotics, citizen consultation, sémiotique, représentations, agrifood models, values, representations, consultation citoyenne, [SHS] Humanities and Social Sciences, modèles agroalimentaires, [SHS]Humanities and Social Sciences
Abstract: Depuis les années 2000, les enjeux sanitaires et environnementaux, ainsi que les préoccupations liées au changement climatique occupent une place importante dans la gestion de la politique agricole (à l’échelle française comme européenne). Ce constat nous conduit à nous intéresser aux États généraux de l’alimentation et particulièrement à la plateforme participative de la consultation citoyenne (ouverte du 20 juillet au 10 novembre 2017). Cette dernière nous paraît être un espace privilégié pour aborder le point de vue de la société civile, ses attentes, ses préoccupations, mais aussi, sa représentation du monde agricole à travers un ensemble de registres de valeurs en circulation. Ainsi, en prenant appui sur la consultation citoyenne, nous nous interrogerons sur les questions suivantes : quelles propositions le panel de citoyen(ne)s retient-il pour répondre à la question « Comment accompagner la transformation de notre agriculture » ? Le climat et la préservation de la biodiversité sont-ils présents, si oui, comment ? Le modèle agricole dominant (l’agriculture dite « conventionnelle ») est-il remis en cause ? Si oui, comment ? Au final, quelle(s) représentation(s) des modèles agricoles les investissements sémantiques que nous relèverons suggèrent-ils ? À partir d’une approche sémiotique, nous tenterons d’apporter des réponses à nos interrogations. Since the 2000s, health and environmental issues, but also the climate change have occupied an important place in the management of agricultural policy (at both the French and European levels). This observation leads us to take an interest in the Food General Assembly and particularly in the participatory platform for citizen (open from July 20 to November 10, 2017). The citizen consultation seems to be a privileged space for approaching the point of view of civil society, its expectations, its concerns, but also its representation of the agricultural world through a set of registers of values in circulation. Thus, based on the citizen consultation, we will question on: what proposals does the citizens' panel retain to answer the question "How to support the transformation of our agriculture"? Are the climate and the preservation of biodiversity present, if so, how? Is the dominant agricultural model (so-called "conventional" agriculture) being in question? and how? In the end, what are the representation(s) of agricultural models from semantic investments? From a semiotic approach, we will try to provide answers to our questions.
Published: 2021

18. Rose wine market: anything but colour?

Author: Stephanie Peres, Eric Giraud-Heraud, Anne-Sophie Masure, Sophie Tempere, Groupe de Recherche en Economie Théorique et Appliquée (GREThA), Centre National de la Recherche Scientifique (CNRS)-Université de Bordeaux (UB), Unité de Recherche Oenologie [Villenave d'Ornon], Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), and Unité de Recherche Oenologie [Villenave d'Ornon] (OENO)
Subjects: Rose wine, No added sulphites, [SDE]Environmental Sciences, lcsh:TP1-1185, Sensory analysis, lcsh:Chemical technology, Article, Experimental market, Colour, [SHS]Humanities and Social Sciences
Abstract: In many countries, the consumption of still wine is in strong decline. The market for rose wine, however, stands in stark contrast to this trend, seeing worldwide growth of almost 30% over the last 15 years. For most observers/experts, product colour plays an important role in this paradigm shift. For this reason, companies&rsquo, marketing efforts often focus on this purely visual characteristic. There is, however, no certainty that other emerging consumer demands, related to environmental concerns or how &ldquo, natural&rdquo, a wine is (organic wines, natural wines, etc.), do not also play a role in the enthusiasm seen in new wine consumers. This article proposes an assessment of expectations related to colour and the decisions made by rose wine consumers, using two complementary experiments carried out in France. The first experiment is based on an online survey studying only consumers&rsquo, colour preferences. We will show that, contrary to popular belief, there is no consensus on this criterion, although regional trends can be identified. Typically, the &ldquo, salmon&rdquo, shade, which is generally the leader on the global market&mdash, and characteristic of Provence wines&mdash, does not win unanimous support across all regions. In contrast, an &ldquo, apricot&rdquo, shade seems to be preferred by consumers in the Bordeaux region. The second experiment confirms this result within the framework of an experimental market revealing consumers&rsquo, willingness to pay (WTP). This market also offers consumers the opportunity to taste wines and provides information on organic certification and &ldquo, naturalness&rdquo, (symbolised by the absence of added sulphites). We will then demonstrate how the latter criteria, although often popular, play only a small role&mdash, compared with colour&mdash, in consumer decisions. We will conclude this article with observations on the atypical nature of the rose wine market and on possible avenues for further research related to the emotional role colour plays in wine tasting and its possible specificity in the world of food and drink products.
Published: 2020
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19. Wine phenolic compounds differently affect the host-killing activity of two lytic bacteriophages infecting the lactic acid bacterium oenococcus oeni

Author: Melina Petrel, Claire Le Marrec, Adeline Goulet, Amel Chaïb, Alain Decendit, Patricia Ballestra, Fety Jaomanjaka, Christian Cambillau, Stéphanie Cluzet, Aurélie Lagarde, Cécile Philippe, Olivier Claisse, Institut des Sciences de la Vigne et du Vin [Villenave d'Ornon] (ISVV), Université de Bordeaux (UB), Unité de Recherche Oenologie [Villenave d'Ornon] (OENO), Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut des Sciences de la Vigne et du Vin (ISVV), Bordeaux Imaging Center (BIC), Université de Bordeaux (UB)-Institut François Magendie-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Architecture et fonction des macromolécules biologiques (AFMB), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Laboratoire d'ingénierie des systèmes macromoléculaires (LISM), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), ANR-10-INBS-0004,France-BioImaging,Développment d'une infrastructure française distribuée coordonnée(2010), ANR-14-CE20-0007,LYSOPLUS,Incidence des bactériophages infectant les bactéries lactiques (Leuconostocaceae) associées à la fabrication de pain au levain, de vin et de fromage et analyse bénéfices-risques de la lysogénie pour un meilleur contrôle des fermentations(2014), ANR-11-IDEX-0001,Amidex,INITIATIVE D'EXCELLENCE AIX MARSEILLE UNIVERSITE(2011), Unité de Recherche Oenologie [Villenave d'Ornon], and Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut François Magendie-Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0301 basic medicine, Coumaric Acids, [SDV]Life Sciences [q-bio], 030106 microbiology, lcsh:QR1-502, Wine, receptor-binding protein, Article, lcsh:Microbiology, Cinnamic acid, Bacteriophage, 03 medical and health sciences, chemistry.chemical_compound, Phenols, bacteriophage, Virology, Bacteriophages, Oenococcus, polyphenols, Winemaking, Oenococcus oeni, Flavonoids, biology, biology.organism_classification, Molecular Docking Simulation, 030104 developmental biology, Infectious Diseases, chemistry, Biochemistry, Lytic cycle, Polyphenol, adsorption, Host-Pathogen Interactions, Myricetin, predation
Abstract: To provide insights into phage-host interactions during winemaking, we assessed whether phenolic compounds modulate the phage predation of Oenococcus oeni. Centrifugal partition chromatography was used to fractionate the phenolic compounds of a model red wine. The ability of lytic oenophage OE33PA to kill its host was reduced in the presence of two collected fractions in which we identified five compounds. Three, namely, quercetin, myricetin and p-coumaric acid, significantly reduced the phage predation of O. oeni when provided as individual pure molecules, as also did other structurally related compounds such as cinnamic acid. Their presence was correlated with a reduced adsorption rate of phage OE33PA on its host. Strikingly, none of the identified compounds affected the killing activity of the distantly related lytic phage Vinitor162. OE33PA and Vinitor162 were shown to exhibit different entry mechanisms to penetrate into bacterial cells. We propose that ligand-receptor interactions that mediate phage adsorption to the cell surface are diverse in O. oeni and are subject to differential interference by phenolic compounds. Their presence did not induce any modifications in the cell surface as visualized by TEM. Interestingly, docking analyses suggest that quercetin and cinnamic acid may interact with the tail of OE33PA and compete with host recognition.
Published: 2020
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20. Silicon carbide (SiC) membranes in œnology: a laboratory-scale study

Author: Philippe Moulin, Lucas Barthélémy, Rémy Ghidossi, Mathilda Trevisan, Laboratoire de Mécanique, Modélisation et Procédés Propres (M2P2), Centre National de la Recherche Scientifique (CNRS)-École Centrale de Marseille (ECM)-Aix Marseille Université (AMU), Unité de Recherche Oenologie [Villenave d'Ornon], Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS), and Unité de Recherche Oenologie [Villenave d'Ornon] (OENO)
Subjects: Materials science, fouling, 02 engineering and technology, Horticulture, lcsh:Agriculture, Bottling line, chemistry.chemical_compound, 0404 agricultural biotechnology, lcsh:Botany, Silicon carbide, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Silicon carbide membrane, Ceramic, wine, Porosity, Wine, Fouling, digestive, oral, and skin physiology, lcsh:S, food and beverages, 04 agricultural and veterinary sciences, Permeation, 021001 nanoscience & nanotechnology, filtration performances, 040401 food science, 6. Clean water, lcsh:QK1-989, Membrane, chemistry, Chemical engineering, visual_art, regeneration, [SDE]Environmental Sciences, visual_art.visual_art_medium, 0210 nano-technology, Food Science
Abstract: Unfiltered wine is a turbid medium that is not generally accepted by the consumer. Therefore, one or several filtration steps are required before bottling. Silicon carbide (SiC) membranes desirable parameters (porosity, tortuosity fluxes) allow filtering several different types of loaded matrices like wine or residue sediment. An in-depth filtration study was carried out on white and red wines to evaluate membrane efficiency and to optimise their cleaning procedure. Retention rates were studied as a function of wine type, filtration mode, and volumetric concentration factor. Compared to ceramic membranes, SiC membrane permeate fluxes are higher, up to a factor of 10 for red wine. For white wines, equivalent permeate fluxes could be obtained with dead-end filtration. Moreover, SiC membranes appear to be effective in obtaining a clear and brilliant wine and do not modify the concentration of the compounds of interest in wine. Finally, an optimised cleaning protocol has been identified and shown to restore a sufficient permeability to the SiC membranes.
Published: 2020
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21. Red wine extract disrupts Th17 lymphocyte differentiation in a colorectal cancer context

Author: Fanny Chalmin, François Ghiringhelli, Emeric Limagne, Cyril Auger, Emma Cantos-Villar, Pauline Chalons, Tristan Richard, Dominique Delmas, Philippe Chabert, Virginie Aires, Flavie Courtaut, Valérie B. Schini-Kerth, Lipides - Nutrition - Cancer [Dijon - U1231] (LNC), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Instituto Andaluz de Investigación y Formación Agraria y Pesquera (IFAPA), Unité de Recherche Oenologie [Villenave d'Ornon], Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV), Nanomédecine Régénérative (NanoRegMed), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de Bioimagerie et Pathologies (LBP), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Centre recherche en CardioVasculaire et Nutrition = Center for CardioVascular and Nutrition research (C2VN), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), ANR-11-LABX-0021,Lipstic,Lipoprotéines et santé : prévention et traitement des maladies inflammatoires non vasculaires et du(2011), Université de Bourgogne (UB), Inserm U1231 University UBFC Dijon, Plateforme de transfert en biologie cancérologique [Dijon], Centre d'épidémiologie des populations (CEP), Université de Bourgogne (UB)-Centre Régional de Lutte contre le cancer Georges-François Leclerc [Dijon] (UNICANCER/CRLCC-CGFL), UNICANCER-UNICANCER-Université de Bourgogne (UB)-Centre Régional de Lutte contre le cancer Georges-François Leclerc [Dijon] (UNICANCER/CRLCC-CGFL), UNICANCER-UNICANCER, Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Département d'oncologie médicale [Centre Georges-François Leclerc], Centre Régional de Lutte contre le cancer Georges-François Leclerc [Dijon] (UNICANCER/CRLCC-CGFL), Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Santé et de la Recherche Médicale (INSERM), Lipides - Nutrition - Cancer (U866) (LNC), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA), Unité de Recherche Oenologie [Villenave d'Ornon] (OENO), and ANR-11-LABX-0021,Lipstic,Lipoprotéines et santé : prévention et traitement des maladies inflammatoires non vasculaires et du cancer(2011)
Subjects: 0301 basic medicine, Cancers polyphenols, red wine extract, Plateforme de Transfert en Biologie du Cancer (PTBC) Chalmin, Wine, Cancers Lipids, [SHS]Humanities and Social Sciences, lymphocyte T Red wine extract, chemoprevention, Lymphocytes, Emeric, Mice, Inbred BALB C, Dominique, Interleukin-17, Lymphocyte differentiation, Vin rouge, Cell Differentiation, Flavie, Santé humaine, Lipids, colon cancer, Female, Interleukin 17, medicine.symptom, Cancers Limagne, Colorectal Neoplasms, Cancers, Cancers Delmas, Biotechnology, OEnologie, Inflammation, Biology, 03 medical and health sciences, Lymphocytes, Tumor-Infiltrating, Immune system, Cell Line, Tumor, Cancers Courtaut, medicine, Animals, Humans, lymphocytes Th17, Cell Proliferation, Nutrition, 030109 nutrition & dietetics, Fanny, Plant Extracts, Interleukins, Polyphenols, HCT116 Cells, Antineoplastic Agents, Phytogenic, Xenograft Model Antitumor Assays, Mice, Inbred C57BL, 030104 developmental biology, Tumor progression, STAT protein, Cancer research, Th17 Cells, Cytokine secretion, Virginie, Inflammasome complex, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, Food Science
Abstract: International audience; Scope: Scope: It is well established that immune response and inflammation promote tumoral progression. Immune cells communicate through direct contact or through cytokine secretion, and it is the pro-inflammatory status that will tip the balance toward tumor progression or anti-tumor immunity. It is demonstrated here that a red wine extract (RWE) can decrease inflammation through its action on the inflammasome complex. This study determines whether an RWE could impact other key actors of inflammation, including T helper 17 (Th17) immune cells in particular. Methods and results: Methods and results: Using an RWE containing 4.16 g of polyphenols/liter of wine, it is shown that RWE decreases colorectal cancer cells in vitro and induces a reduction in colorectal tumor growth associated with a decrease in tumor-infiltrating lymphocytes in vivo. The process of T-lymphocyte differentiation in Th17 cells is altered by RWE, as revealed by the decrease in the expression of key actors controlling this process, such as signal transducer and activator of transcription 3 and retinoid acid-related orphan receptor t. This disruption is associated with an inhibition of inflammatory interleukin 17 secretion. Conclusion: The data highlights the major involvement of Th17 immune cells in the biological effects of an RWE.
Published: 2020
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22. 'French Phage Network' Annual Conference—Fifth Meeting Report

Author: Amel Chaïb, Romain Linares, Cécile Breyton, Floriane Laumay, Genomic Research Laboratory [Geneva, Switzerland], Geneva University Hospital (HUG), Unité de Recherche Oenologie [Villenave d'Ornon] (OENO), Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut de biologie structurale (IBS - UMR 5075), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA), ANR-16-CE11-0027,PerfoBac,Comment les bactériophages perforent-ils la paroi des bactéries ?(2016), Unité de Recherche Oenologie [Villenave d'Ornon], and Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)
Subjects: 0301 basic medicine, bacteriophages, media_common.quotation_subject, 030106 microbiology, lcsh:QR1-502, Ecology and Evolution, Library science, Atmosphere (architecture and spatial design), lcsh:Microbiology, Pleasure, 03 medical and health sciences, Phage– Structure and Assembly, Virology, Political science, Humans, Phage Therapy and Biotechnology, Phage Therapy, media_common, ddc:616, Bacteriophages/physiology/ultrastructure, [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], Phage–Structure and Assembly, Conference Report, Host Interaction, Biological Evolution, 030104 developmental biology, Infectious Diseases, Host-Pathogen Interactions, Biotechnology
Abstract: International audience; Attracting about 100 participants, the fifth edition of our French Phages.fr annual conference was once more a success. This year's conference took place at the Institute for Structural Biology on the European Electron and Photon Campus in Grenoble, October 8 and 9, 2019. Similar to previous years, our meeting gathered scientists mainly working in France, from academic labs and hospitals as well as from industry. We also had the pleasure of welcoming attendees from different European countries and even beyond. The conference was divided into four sessions: Ecology and Evolution, Phage Therapy and Biotechnology, Structure and Assembly and Phage-Host Interaction, each opened by a keynote lecture. The talks, selected from abstracts, gave the opportunity for young scientists (especially students and post-docs) to present their project and results in a friendly atmosphere. Poster sessions also favoured interactions and discussions between young researchers and more senior scientists. Here, we provide a summary of the topics developed during the conference.
Published: 2020
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23. Production of Fungal Nanochitosan Using High-Pressure Water Jet System for Biomedical Applications

Author: Kota Ogura, Clément Brasselet, Gustavo Cabrera-Barjas, Masoud Hamidi, Amin Shavandi, Marguerite Dols-Lafargue, Naoki Sawamura, Cédric Delattre, Institut national polytechnique Clermont Auvergne (INP Clermont Auvergne), Université Clermont Auvergne (UCA), Universidad de Concepción - University of Concepcion [Chile], Université libre de Bruxelles (ULB), Unité de Recherche Œnologie [Villenave d'Ornon] (OENO), Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut Universitaire de France (IUF), Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.), and ANR-17-CE21-0006,CHITOWINE,Recherches sur l'origine et les effets secondaires des propriétés stabilisantes du chitosane fongique dans le vin(2017)
Subjects: fungal chitosan, nanofiber, high-pressure water jet system, antimicrobial film, antioxidant film, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, General Materials Science
Abstract: International audience; In this present work, fungal nanochitosans, with very interesting particle size distribution of 22 µm, were efficiently generated in high-yield production using a high-pressure water jet system (Star Burst System, Sugino, Japan) after 10 passes of mechanical treatment under high pressure. The specific characterization of fungal chitosan nanofibers suspensions in water revealed a high viscosity of 1450 mPa.s and an estimated transparency of 43.5% after 10 passes of fibrillation mechanical treatment. The mechanical characterization of fungal nanochitosan (NC) film are very interesting for medical applications with a Young’s modulus (E), a tensile strength (TS), and elongation at break (e%) estimated at 2950 MPa, 50.5 MPa, and 5.5%, respectively. Furthermore, we exhibited that the fungal nanochitosan (NC) film presented very good long-term antioxidant effect (reached 82.4% after 96 h of contact with DPPH radical solution) and very interesting antimicrobial activity when the nanochitosan (NC) fibers are mainly activated as NC-NH3+ form at the surface of the film with 45% reduction and 75% reduction observed for S. aureus (Gram-positive) and E. coli (Gram-negative), respectively, after 6 h of treatment. These promising antimicrobial and antioxidant activities indicated the high potential of valorization toward biomedical applications.
Published: 2022
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24. Silicon carbide (SiC) membranes in œnology

Author: Trevisan, M., Ghidossi, R., Moulin, P., Laboratoire de Mécanique, Modélisation et Procédés Propres (M2P2), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS), Unité de Recherche Œnologie [Villenave d'Ornon] (OENO), and Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
Subjects: [SPI]Engineering Sciences [physics], [CHIM.GENI]Chemical Sciences/Chemical engineering, [SDV.IDA]Life Sciences [q-bio]/Food engineering, Membrane, Wine, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, Filtration and Separation, Silicon carbide, Fouling, Silicon carbide, Membrane, Filtration, Wine, Fouling, Filtration, Analytical Chemistry
Abstract: International audience; In a winery, clarification and stabilization steps by crossflow microfiltration are limited by fouling phenomenon which reduces production flows and can alter the aromatic potential of wines, forcing professionals to use several successive filtration steps. The objectives of this industrial-scale study are to (i) introduce SiC membranes at different vinification steps (ii) evaluate the hydraulic and retention performances of SiC membranes and (iii) carry out a comparative study between SiC membranes and polymeric hollow fibers commonly used in the wine industry. It appears that SiC membranes are able to filter diverse matrices with very satisfactory production flow and retention efficiency. This particular process was optimized by developing an efficient regeneration protocol. The clarification and stabilization of the matrices by SiC membranes allowed (i) the production of clear and brilliant wines, (ii) the retention of wine microorganisms, (iii) higher production flows than hollow fibers, while conserving wines interest compounds, (iv) and the filtration of matrices that cannot be processed by hollow fibers.
Published: 2022
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25. Physical, Anatomical, and Biochemical Composition of Skins Cell Walls from Two Grapevine Cultivars (Vitis vinifera) of Champagne Region Related to Their Susceptibility to Botrytis cinerea during Ripening

Author: Audrey Barsacq, Laurence Geny-Denis, Etienne Gontier, Diane Courot, Melina Petrel, Laurence Mercier, Soizic Lacampagne, Marie André, Unité de Recherche Oenologie [Villenave d'Ornon], Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut des Sciences de la Vigne et du Vin [Villenave d'Ornon] (ISVV), Université de Bordeaux (UB), Moët Hennessy Champagne Services (MHCS), Bordeaux Imaging Center (BIC), Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut François Magendie-Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), DESAILLY, Marion, Unité de Recherche Oenologie [Villenave d'Ornon] (OENO), and Université de Bordeaux (UB)-Institut François Magendie-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
Subjects: [SDE] Environmental Sciences, 0106 biological sciences, [SDV]Life Sciences [q-bio], polysaccharides, champagne, Plant Science, Horticulture, Polysaccharide, 01 natural sciences, SB1-1110, Cell wall, Botrytis cinerea, 03 medical and health sciences, Biochemical composition, Cultivar, Vitis vinifera, 030304 developmental biology, chemistry.chemical_classification, 0303 health sciences, integumentary system, biology, grape skin, fungi, Plant culture, food and beverages, Ripening, biology.organism_classification, [SDV] Life Sciences [q-bio], chemistry, [SDE]Environmental Sciences, cell wall, Composition (visual arts), 010606 plant biology & botany
Abstract: This work investigated the structural, biochemical, and molecular characteristics of grape skin cell wall during ripening, related to susceptibility to Botrytis cinerea. The comparative study between the two main grape cultivars in Champagne region, Pinot noir and Chardonnay, quantified: (1) the maturity and physical profile of grape skin, (2) the morphological characteristics, (3) soluble pectic polysaccharides located in grape skin cell walls, and (4) the gene expression of the two main degrading enzymes (VvPME1 and VvPG1) and PME activity. During the maturation period, the grape skins of the two cultivars appear different in their structure and composition. Chardonnay is characterized by higher relative humidity (RH) and level of VvPG1 expression, lower disease incidence and penetrometry values, and thicker cell walls than Pinot noir skins. Thus, the cell wall composition is sufficiently different between grape varieties from the same area to allow their discrimination and could be used to better manage the harvest date.
Published: 2021
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26. Masking of Several Olfactory Notes by Infra-threshold Concentrations of 2,4,6-Trichloroanisole

Author: Sophie Tempere, E. Cuzange, G. de Revel, M.H. Schaaper, Gilles Sicard, Unité de Recherche Oenologie [Villenave d'Ornon], Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV), Quali-Bordeaux, Neurobiologie des interactions cellulaires et neurophysiopathologie - NICN (NICN), Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS), Institut des Sciences de la Vigne et du Vin (ISVV)-Université de Bordeaux (UB)-Institut National de la Recherche Agronomique (INRA), and Unité de Recherche Oenologie [Villenave d'Ornon] (OENO)
Subjects: 0301 basic medicine, 2,4,6-Trichloroanisole, [SDV]Life Sciences [q-bio], Stimulation, olfactory masking, cork taint, Sensory analysis, sensory analysis, 03 medical and health sciences, Cellular and Molecular Neuroscience, chemistry.chemical_compound, Perceived quality, dichorhinic stimulation, vitis vinifera, 0302 clinical medicine, analyse sensorielle, Wine, Psychophysical tests, wine profiling, Communication, chromatographie, business.industry, infra-threshold (subliminal) concentrations, Sensory Systems, 030104 developmental biology, oenologie, chemistry, Odor, chromatography, Cork taint, business, Psychology, Biological system, 030217 neurology & neurosurgery
Abstract: Introduction Among wine defects, 2,4,6-trichloroanisole has a specific impact on wine perception. In addition to giving to the wine an unpleasant odor, 2,4,6-trichloroanisole has a masking effect on notes. In this study, the specificity and efficacy of the masking effect of 2,4,6-trichloroanisole was tested at infra- and supra-threshold concentrations. A simplified model of a binary mixture was also studied for in-depth analysis of this phenomenon.Methods Techniques used included sensory analysis (odor profiling), psychophysical tests (threshold measurement and triangle test), chemical analyses (GC-MS), and a mono/dichorhinic stimulation paradigm, to test the hypothesis of peripheral interaction.Results The results revealed that TCA had a masking effect on a range of aromatic notes, even at infra-threshold concentrations. However, they also showed counteraction of odorant specificity by 2,4,6-trichloroanisole. The origin of this masking effect was also discussed. The results suggested that this interaction can be assumed to take place at receptor level.Conclusion This study provided experimental confirmation of the widespread idea that constituents in non-perceptible concentrations influence the perceived quality of mixtures of odorous compounds.Implications Moreover, this type of olfactory model may improve our understanding of combinatorial olfactory encoding at the peripheral level.
Published: 2017
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27. Brettanomyces bruxellensis Displays Variable Susceptibility to Chitosan Treatment in Wine

Author: Cédric Delattre, Joana Coulon, Lucie Dutilh, Margot Paulin, Pascal Dubessay, Cécile Miot-Sertier, Marguerite Dols-Lafargue, Clément Brasselet, Julie Maupeu, Thierry Doco, Warren Albertin, Amélie Vallet-Courbin, Patricia Ballestra, Virginie Moine, Guillaume Pierre, Philippe Michaud, Isabelle Masneuf-Pomarède, Unité de Recherche Oenologie [Villenave d'Ornon], Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Microflora, Institut Pascal - Clermont Auvergne (IP), Sigma CLERMONT (Sigma CLERMONT)-Université Clermont Auvergne (UCA)-Centre National de la Recherche Scientifique (CNRS), Institut Universitaire de France (IUF), Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.), Sciences Pour l'Oenologie (SPO), Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité de Recherche Oenologie [Villenave d'Ornon] (OENO), Institut Pascal (IP), SIGMA Clermont (SIGMA Clermont)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), Université de Montpellier (UM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro - Montpellier SupAgro, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), and Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)
Subjects: Microbiology (medical), [SDV]Life Sciences [q-bio], Population, Food spoilage, lcsh:QR1-502, Brettanomyces bruxellensis, Microbial agent, Microbiology, Lees, lcsh:Microbiology, Chitosan, 03 medical and health sciences, chemistry.chemical_compound, Food science, wine, education, 030304 developmental biology, Wine, 0303 health sciences, education.field_of_study, biology, 030306 microbiology, Chemistry, spoilage, food and beverages, biology.organism_classification, Yeast, volatile phenols, antiseptic, chitosan
Abstract: International audience; Brettanomyces bruxellensis is the main spoilage microbial agent in red wines. The use of fungal chitosan has been authorized since 2009 as a curative treatment to eliminate this yeast in conventional wines and in 2018 in organic wines. As this species is known to exhibit great genetic and phenotypic diversity, we examined whether all the strains responded the same way to chitosan treatment. A collection of 53 strains of B. bruxellensis was used. In the conditions of the reference test, all were at least temporarily affected by the addition of chitosan to wine, with significant decrease of cultivable population. Some (41%) were very sensitive and no cultivable yeast was detected in wine or lees after 3 days of treatment, while others (13%) were tolerant and, after a slight drop in cultivability, resumed growth between 3 and 10 days and remained able to produce spoilage compounds. There were also many strains with intermediate behavior. The strain behavior was only partially linked to the strain genetic group. This behavior was little modulated by the physiological state of the strain or the dose of chitosan used (within the limits of the authorized doses). On the other hand, for a given strain, the sensitivity to chitosan treatment was modulated by the chitosan used and by the properties of the wine in which the treatment was carried out.
Published: 2020
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28. Diastereomeric stilbenoid glucoside dimers from the rhizomes of Gnetum africanum

Author: Jonathan Bisson, Jean-Michel Mérillon, Caroline Rouger, Julien Gabaston, Thierry Buffeteau, Pierre Waffo-Teguo, Thierry Brotin, Institut des Sciences de la Vigne et du Vin [Villenave d'Ornon] (ISVV), Université de Bordeaux (UB), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut des Sciences Moléculaires (ISM), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Chimie - UMR5182 (LC), Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-École normale supérieure - Lyon (ENS Lyon)-Institut de Chimie du CNRS (INC), Unité de Recherche Oenologie [Villenave d'Ornon] (OENO), Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1 (UB)-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Circular dichroism, Stereochemistry, Plant Science, [CHIM.THER]Chemical Sciences/Medicinal Chemistry, Stilbenoid, 01 natural sciences, Biochemistry, chemistry.chemical_compound, Glucoside, Stilbenoids, Gnetum africanum, Chelation, Absolute configuration, [CHIM.COOR]Chemical Sciences/Coordination chemistry, biology, 010405 organic chemistry, Diastereomer, Polyphenols, biology.organism_classification, 0104 chemical sciences, 3. Good health, Rhizome, 010404 medicinal & biomolecular chemistry, chemistry, Gnetaceae, Vibrational circular dichroism, Iron chelation, Agronomy and Crop Science, Biotechnology
Abstract: International audience; In a continuing search for neuroprotective stilbenoids, two uncommon diastereomeric stilbenoid glucosides, africanoside A (1) and B (2) as well as fifteen known stilbenes, E-resveratrol, E-gnetifolin E, E-piceid, E-resveratroloside, E-gnetifolin K, E-gnetol, E-isorhapontigenin, E-isorhapontin, E-isorhapontigenin-4′-O-glucopyranoside, E-gnetin C, E-bisisorhapontigenin B, E-gnemonoside A, E-gnemonoside C, E-gnemonoside D, and E-gnetin E, were isolated from rhizomes of Gnetum africanum, using a combination of centrifugal partition chromatography and preparative HPLC. The structure of these two stilbenoids was investigated by NMR, vibrational circular dichroism (VCD), and electronic circular dichroism (ECD) experiments. The absolute configurations of 1, and 2 were established by VCD as (7aS,8aS) and (7aR,8aR), respectively. Compound 1 significantly chelated Fe(II) at 100, 250 and 500 μM. The ability of stilbenoids to chelate ferrous ions which are implicated in physiopathological hallmark of Alzheimer’s disease provides useful data for potential treatment.
Published: 2020
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29. Natural allelic variations of Saccharomyces cerevisiae impact stuck fermentation due to the combined effect of ethanol and temperature; a QTL-mapping study

Author: Marullo, Philippe, Durrens, Pascal, Peltier, Emilien, Bernard, Margaux, Mansour, Chantal, Dubourdieu, Denis, Faculté d'Oenologie [Bordeaux II], Université Bordeaux Segalen - Bordeaux 2-Institut des Sciences de la Vigne et du Vin (ISVV), BioLaffort [Floirac], Laboratoire Bordelais de Recherche en Informatique (LaBRI), Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2, from patterns to models in computational biodiversity and biotechnology (PLEIADE), Biodiversité, Gènes & Communautés (BioGeCo), Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Inria Bordeaux - Sud-Ouest, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-Laboratoire Bordelais de Recherche en Informatique (LaBRI), Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2, Unité de Recherche Oenologie [Villenave d'Ornon], Institut des Sciences de la Vigne et du Vin (ISVV)-Université de Bordeaux (UB)-Institut National de la Recherche Agronomique (INRA), Philippe Marullo received 2 grants from Conseil Regional d’Aquitaine (SAGESSE forgenome sequencing, and QTL2 for genotyping). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript., Université de Bordeaux (UB)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Centre National de la Recherche Scientifique (CNRS), Université de Bordeaux (UB)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Centre National de la Recherche Scientifique (CNRS)-Université de Bordeaux (UB)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Centre National de la Recherche Scientifique (CNRS)-Inria Bordeaux - Sud-Ouest, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-Biodiversité, Gènes & Communautés (BioGeCo), Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut National de la Recherche Agronomique (INRA), Unité de Recherche Oenologie [Villenave d'Ornon] (OENO), Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB), and Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Inria Bordeaux - Sud-Ouest
Subjects: lcsh:QH426-470, VHS1, Ethanol, OYE2, QTL, Subtelomeric region, Temperature, Wine yeast, lcsh:Biotechnology, Quantitative Trait Loci, Wine, Saccharomyces cerevisiae, Protein Serine-Threonine Kinases, lcsh:TP248.13-248.65, Alleles, Whole Genome Sequencing, NADPH Dehydrogenase, food and beverages, Chromosome Mapping, lcsh:Genetics, Phenotype, Fermentation, Ethano, [INFO.INFO-BI]Computer Science [cs]/Bioinformatics [q-bio.QM], Sugars, Research Article
Abstract: Background Fermentation completion is a major prerequisite in many industrial processes involving the bakery yeast Saccharomyces cerevisiae. Stuck fermentations can be due to the combination of many environmental stresses. Among them, high temperature and ethanol content are particularly deleterious especially in bioethanol and red wine production. Although the genetic causes of temperature and/or ethanol tolerance were widely investigated in laboratory conditions, few studies investigated natural genetic variations related to stuck fermentations in high gravity matrixes. Results In this study, three QTLs linked to stuck fermentation in winemaking conditions were identified by using a selective genotyping strategy carried out on a backcrossed population. The precision of mapping allows the identification of two causative genes VHS1 and OYE2 characterized by stop-codon insertion. The phenotypic effect of these allelic variations was validated by Reciprocal Hemyzygous Assay in high gravity fermentations (> 240 g/L of sugar) carried out at high temperatures (> 28 °C). Phenotypes impacted were mostly related to the late stage of alcoholic fermentation during the stationary growth phase of yeast. Conclusions Our findings illustrate the complex genetic determinism of stuck fermentation and open new avenues for better understanding yeast resistance mechanisms involved in high gravity fermentations. Electronic supplementary material The online version of this article (10.1186/s12864-019-5959-8) contains supplementary material, which is available to authorized users.
Published: 2019
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30. Impact of polyphenols on receptor-ligand interactions by NMR the case of neurotensin (NT)-neurotensin receptor fragment (NTS1) complex

Author: Stéphane Quideau, Arnaud Bondon, Tahiri Sylla, Grégory Da Costa, Jean-Pierre Monti, Jean-Michel Mérillon, Eva Petit, Tristan Richard, Laurent Pouységu, Unité de Recherche Oenologie [Villenave d'Ornon], Institut des Sciences de la Vigne et du Vin (ISVV)-Université de Bordeaux (UB)-Institut National de la Recherche Agronomique (INRA), Physiologie Pathologie et Génétique Végétales (PPGV), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Institut des Sciences Moléculaires (ISM), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de physique et biophysique, Université Bordeaux Segalen - Bordeaux 2, Institut des Sciences Chimiques de Rennes (ISCR), Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Supérieure de Chimie de Rennes-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES), ANR-11-INBS-0010, Bordeaux Metabolome Facility, and MetaboHUB, Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), Unité de Recherche Oenologie [Villenave d'Ornon] (OENO), Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Physiologie, Pathologie et Génétique Végétales (PPGV), Ecole d'Ingénieurs de Purpan (INP - PURPAN), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1 (UB)-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and ANR-11-INBS-0010,METABOHUB,Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation(2011)
Subjects: Models, Molecular, 030303 biophysics, interaction, phenolic compounds, Receptor-ligand complex, Ligands, 03 medical and health sciences, chemistry.chemical_compound, Structural Biology, Receptors, Neurotensin, [CHIM]Chemical Sciences, neurodegenerative diseases, Neurotensin receptor, Receptor, receptor–ligand complex, Molecular Biology, Neurotensin, ComputingMilieux_MISCELLANEOUS, 0303 health sciences, fungi, food and beverages, Polyphenols, General Medicine, Ligand (biochemistry), NMR, 3. Good health, Biochemistry, chemistry, Polyphenol
Abstract: International audience; Ligand-receptor interactions can be implicated in many pathological events such as chronic neurodegenerative diseases. Thus, the discovery of molecules disrupting this type of interactions could be an interesting therapeutic approach. Polyphenols are well known for their affinity for proteins and several studies have characterized these direct interactions. But studying the direct influence of multi-therapeutic drugs on a ligand-receptor complex relevant to a neurodegenerative disorder is a challenging issue. Solution NMR, molecular modeling and iterative calculations were used to obtain information about the interaction between a phenolic compound, α-glucogallin (α-2) and a ligand/fragment receptor complex neurotensin (NT) and its receptor NTS1. The α-2 was shown to bind to NT and a peptidic fragment of its NTS1 receptor, independently. Although the formation of the corresponding ligand-receptor complex did not seem to be affected, this experimental modeling protocol will enable the evaluation of other anti-amyloidogenic compounds such as blockers of NT-NTS1 binding. These types of studies help in understanding the specificity and influence in binding and can provide information to develop new molecules with a putative pharmacological interest.
Published: 2019
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31. Consumer preferences for different red wine styles and repeated exposure effects

Author: Philippe Darriet, Eric Giraud-Heraud, Alexandre Pons, Stéphanie Peres, Alejandro Fuentes Espinoza, Sophie Tempere, Unité de Recherche Oenologie [Villenave d'Ornon], Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Groupe de Recherche en Economie Théorique et Appliquée (GREThA), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), Unité de Recherche Oenologie [Villenave d'Ornon] (OENO), Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV), Institut National de la Recherche Agronomique (INRA), and Centre National de la Recherche Scientifique (CNRS)-Université de Bordeaux (UB)
Subjects: 030309 nutrition & dietetics, media_common.quotation_subject, Wine, Sensory analysis, Experimental market, [SHS]Humanities and Social Sciences, 03 medical and health sciences, 0404 agricultural biotechnology, Willingness to pay, [SDV.IDA]Life Sciences [q-bio]/Food engineering, Quality (business), [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, Marketing, Aroma, Winemaking, media_common, Consumer evaluation, 2. Zero hunger, 0303 health sciences, Nutrition and Dietetics, [QFIN]Quantitative Finance [q-fin], digestive, oral, and skin physiology, food and beverages, 04 agricultural and veterinary sciences, 040401 food science, Preference, High alcohol, Wine tasting, Psychology, Food Science
Abstract: International audience; As the complexity of wine flavor is strongly linked with grape quality, depending on grape-growing practices and winemaking procedures, winemakers are concerned to anticipate some stabilization of consumer preferences for specific, intrinsic, wine characteristics. The objective of our experiment was to compare the impact of tasting wine over time at home, with first impressions in a single tasting session under laboratory conditions on the preference for certain types of premium red Bordeaux wines available on the market. To assess the consistency of evaluation at home, half of the participants tasted two contrasting wines (fresh/jammy fruit, low/high alcohol) on several occasions. In both cases, traditional sensory analysis and experimental economic techniques were applied to evaluate the preferences of French consumers (N = 184) and their willingness to pay. This observation of the effect of repeated exposure to wines with high alcohol levels (15% vol.), marked by jammy fruit flavors, on the stability of appreciation of these wines clearly revealed the fragility of short-term preferences and the importance of giving consumers time to evaluate wines in a familiar atmosphere.
Published: 2019
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32. Impact of learning and training on wine expertise: a review

Author: Gilles Sicard, Sophie Tempere, G. de Revel, Unité de Recherche Oenologie [Villenave d'Ornon] (OENO), Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV), Neurobiologie des interactions cellulaires et neurophysiopathologie - NICN (NICN), Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), and Unité de Recherche Oenologie [Villenave d'Ornon]
Subjects: 0301 basic medicine, 030109 nutrition & dietetics, Process (engineering), media_common.quotation_subject, Cognition, 04 agricultural and veterinary sciences, 040401 food science, Applied Microbiology and Biotechnology, 03 medical and health sciences, 0404 agricultural biotechnology, Categorization, Perception, [SDV.IDA]Life Sciences [q-bio]/Food engineering, Psychophysics, Quality (business), [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, Wine tasting, Psychology, Food Science, media_common, Mental image, Cognitive psychology
Abstract: International audience; Experts are able to conduct both analytical and holistic tasks involved in evaluating wine quality. Although there is no standard for wine-tasting training, a number of papers have reported on experts' ability to discriminate among and categorize wines, highlighting a process involving perceptual clues and cognitive processing. This review focuses on the influence of different training tasks on tasting and olfactory abilities. Moreover, recent researches in psychophysics and neurosciences demonstrated the potential functionality of mental imagery. The ability to create mental images may play an important role in discrimination and categorization, but is dependent on preliminary perceptual, attentional, and semantic training.
Published: 2019
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33. Chondroprotective properties of human-enriched serum following polyphenol extract absorption: results from an exploratory clinical trial

Author: Ruth Hornedo-Ortega, Stéphanie Krisa, Nelly Urban, Nathalie Meunier, Elsa Mevel, Henri Granel, Juliette Berger, Laurent Beck, Tristan Richard, Jérôme Guicheux, Yohann Wittrant, Line Boutin-Wittrant, Stéphane Descamps, Josep Valls, Claire Vinatier, Adeline Blot, Fabien Wauquier, Wittrant, Yohann, Unité de Nutrition Humaine (UNH), Clermont Université-Université d'Auvergne - Clermont-Ferrand I (UdA)-Institut National de la Recherche Agronomique (INRA), Regenerative Medicine and Skeleton research lab (RMeS), Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), Unité de Recherche Oenologie [Villenave d'Ornon], Institut des Sciences de la Vigne et du Vin (ISVV)-Université de Bordeaux (UB)-Institut National de la Recherche Agronomique (INRA), Modulation Artificielle des Genes Eucaryotes, Université Bordeaux Segalen - Bordeaux 2-Institut National de la Santé et de la Recherche Médicale (INSERM), Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), Union Grap'Sud, Ortho-Traumatologie, Chirurgie plastique et reconstructive, Centre hospitalier universitaire Clermont Ferrand, Université de Nantes - UFR Odontologie, Université de Nantes (UN), CRNH Auvergne, Centre Hospitalier Universitaire de Clermont-Ferrand, Centre de Recherche en Nutrition Humaine d'Auvergne (CRNH d'Auvergne), Jehan, Frederic, Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Regenerative Medicine and Skeleton (RMeS), École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Unité de Recherche Oenologie [Villenave d'Ornon] (OENO), Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV), Role of intra-Clonal Heterogeneity and Leukemic environment in ThErapy Resistance of chronic leukemias (CHELTER), Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Service d’Orthopédie Traumatologie [CHU Clermont-Ferrand], CHU Gabriel Montpied [Clermont-Ferrand], CHU Clermont-Ferrand-CHU Clermont-Ferrand, Centre de Recherche en Nutrition Humaine Auvergne [CHU Clermont-Ferrand] (CRNH A), Direction de la recherche clinique et de l’innovation [CHU Clermont-Ferrand] (DRCI), Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM), CHU Clermont-Ferrand, and Université de Nantes - UFR Odontologie (UFR Odonto)
Subjects: Male, 0301 basic medicine, [SDV.MHEP.HEM] Life Sciences [q-bio]/Human health and pathology/Hematology, Interleukin-1beta, Anti-Inflammatory Agents, Osteoarthritis, Pharmacology, chemistry.chemical_compound, osteoarthritis (OA), cell biology, Ingestion, Cells, Cultured, ComputingMilieux_MISCELLANEOUS, [SDV.MHEP.RSOA] Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, Nutrition and Dietetics, [SDV.MHEP.GEG] Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, Chemistry, [SDV.MHEP.GEG]Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, NF-kappa B, [SDV.MHEP.HEM]Life Sciences [q-bio]/Human health and pathology/Hematology, Phenylethyl Alcohol, procyanidins, Healthy Volunteers, 3. Good health, medicine.anatomical_structure, [SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, Alimentation et Nutrition, hydroxytyrosol, Adult, [SDV.MHEP.AHA] Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], Article, Chondrocyte, Young Adult, 03 medical and health sciences, Chondrocytes, In vivo, medicine, [SDV.MHEP.AHA]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], Humans, Food and Nutrition, Proanthocyanidins, micronutrients, clinical trials, 030109 nutrition & dietetics, Grape Seed Extract, Plant Extracts, Polyphenols, medicine.disease, In vitro, 030104 developmental biology, Absorption, Physicochemical, Polyphenol, Hydroxytyrosol, Ex vivo, Food Science
Abstract: Polyphenols are widely acknowledged for their health benefits, especially for the prevention of inflammatory and age-related diseases. We previously demonstrated that hydroxytyrosol (HT) and procyanidins (PCy), alone or in combination, drive preventive anti-osteoathritic effects in vivo. However, the lack of sufficient clinical evidences on the relationship between dietary phytochemicals and osteoarthritis remains. In this light, we investigated in humans the potential osteoarticular benefit of a grapeseed and olive extract (OPCO) characterized for its hydroxytyrosol (HT) and procyanidins (PCy) content. We first validated, in vitro, the anti-inflammatory and chondroprotective properties of the extract on primary cultured human articular chondrocytes stimulated by interleukin-1 beta (IL-1 &beta, ). The sparing effect involved a molecular mechanism dependent on the nuclear transcription factor-kappa B (NF-&kappa, B) pathway. To confirm the clinical relevance of such a nutritional strategy, we designed an innovative clinical approach taking into account the metabolites that are formed during the digestion process and that appear in circulation after the ingestion of the OPCO extract. Blood samples from volunteers were collected following ingestion, absorption, and metabolization of the extract and then were processed and applied on human primary chondrocyte cultures. This original ex vivo methodology confirmed at a clinical level the chondroprotective properties previously observed in vitro and in vivo.
Published: 2019
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34. Modification of Chitosan for the Generation of Functional Derivatives

Author: Thierry Doco, Julie Maupeu, Pascal Dubessay, Amélie Vallet-Courbin, Marguerite Dols-Lafargue, Hélène de Baynast, Clément Brasselet, Joana Coulon, Philippe Michaud, Guillaume Pierre, Cédric Delattre, Institut Pascal (IP), SIGMA Clermont (SIGMA Clermont)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), Unité de Recherche Oenologie [Villenave d'Ornon] (OENO), Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV), Bio-Laffort, Sciences Pour l'Oenologie (SPO), Université Montpellier 1 (UM1)-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut Universitaire de France (IUF), Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.), ANR-17-CE21-0006,CHITOWINE,Recherches sur l'origine et les effets secondaires des propriétés stabilisantes du chitosane fongique dans le vin(2017), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Sigma CLERMONT (Sigma CLERMONT)-Centre National de la Recherche Scientifique (CNRS), Unité de Recherche oenologie, ISVV, Ecole Nationale Supérieure de Chimie, de Biologie et de Physique (ENSCBP), ADERA-MICROFLORA, Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), ANR CHITOWINE project [ANR-17-CE21-0006-02], French Agence Nationale de la Recherche, Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), SIGMA Clermont (SIGMA Clermont)-Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Unité de Recherche Oenologie [Villenave d'Ornon], Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), and ANR-17-CE21-0006,CHITOWINE,Recherches sur l’origine et les effets secondaires des propriétés stabilisantes du chitosane fongique dans le vin(2017)
Subjects: Bioadhesive, Nanotechnology, 02 engineering and technology, Polysaccharide, chitin, lcsh:Technology, lcsh:Chemistry, Chitosan, 03 medical and health sciences, chemistry.chemical_compound, Chitin, Biological property, Autre (Chimie), General Materials Science, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, lcsh:QH301-705.5, Instrumentation, 030304 developmental biology, Fluid Flow and Transfer Processes, chemistry.chemical_classification, 0303 health sciences, functional properties, chitosan, polysaccharide, bioactivity, lcsh:T, Process Chemistry and Technology, General Engineering, Polymer, Biodegradation, 021001 nanoscience & nanotechnology, chitosane, lcsh:QC1-999, Computer Science Applications, lcsh:Biology (General), lcsh:QD1-999, chemistry, lcsh:TA1-2040, chitine, Other, lcsh:Engineering (General). Civil engineering (General), 0210 nano-technology, [CHIM.OTHE]Chemical Sciences/Other, lcsh:Physics, Wine industry
Abstract: Today, chitosan (CS) is probably considered as a biofunctional polysaccharide with the most notable growth and potential for applications in various fields. The progress in chitin chemistry and the need to replace additives and non-natural polymers with functional natural-based polymers have opened many new opportunities for CS and its derivatives. Thanks to the specific reactive groups of CS and easy chemical modifications, a wide range of physico-chemical and biological properties can be obtained from this ubiquitous polysaccharide that is composed of β-(1,4)-2-acetamido-2-deoxy-d-glucose repeating units. This review is presented to share insights into multiple native/modified CSs and chitooligosaccharides (COS) associated with their functional properties. An overview will be given on bioadhesive applications, antimicrobial activities, adsorption, and chelation in the wine industry, as well as developments in medical fields or biodegradability.
Published: 2019
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35. Resistant grape varieties and market acceptance: an evaluation based on experimental economics

Author: Anne Hubert, Yann Raineau, Eric Giraud-Heraud, Céline Franc, Alejandro Fuentes Espinoza, Groupe de Recherche en Economie Théorique et Appliquée (GREThA), Centre National de la Recherche Scientifique (CNRS)-Université de Bordeaux (UB), Institut des Sciences de la Vigne et du Vin [Villenave d'Ornon] (ISVV), Université de Bordeaux (UB), Institut des Sciences de la Vigne et du Vin (ISVV), Institut National de la Recherche Agronomique (INRA), Unité de Recherche Oenologie [Villenave d'Ornon], Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), and Unité de Recherche Oenologie [Villenave d'Ornon] (OENO)
Subjects: 0301 basic medicine, Vintage, treatment frequency indicator, media_common.quotation_subject, Horticulture, [SHS]Humanities and Social Sciences, lcsh:Agriculture, 03 medical and health sciences, Agricultural science, pesticide residue analysis, Willingness to pay, lcsh:Botany, Quality (business), Market share, wine, experimental economics, media_common, 2. Zero hunger, Wine, 030109 nutrition & dietetics, Mildew, biology, JEL: Q - Agricultural and Natural Resource Economics • Environmental and Ecological Economics/Q.Q1 - Agriculture, lcsh:S, [SDV.SA.AEP]Life Sciences [q-bio]/Agricultural sciences/Agriculture, economy and politics, 04 agricultural and veterinary sciences, Experimental economics, biology.organism_classification, [SHS.ECO]Humanities and Social Sciences/Economics and Finance, lcsh:QK1-989, 040103 agronomy & agriculture, 0401 agriculture, forestry, and fisheries, Wine tasting, Business, resistant varieties, willingness to pay, Food Science
Abstract: International audience; We analyze consumers' evaluations of white wines from resistant varieties, produced in the Languedoc winegrowing region of France (2016 vintage). We use the results from a laboratory experiment performed in Paris in June 2017, where a panel of more than one hundred and sixty consumers, regular buyers of this type of wine, were asked to evaluate a wine of the Bouquet 3159 grape variety (monogenic variety resistant to mildew and powdery mildew and optimized for quality) and compare it with two conventional wines of different quality levels, and with a certified organic wine of similar type and price. The environmental and health performances and the production methods of the different wines were quantified according to several indicators: Treatment frequency indicator (TFI) and pesticide residue analysis. The consumers first evaluated the wines after tasting, having been given only a minimum amount of information about the region of origin and the vintage, then again after receiving information on production methods and the levels of our indicators. The method used to lend credibility to individual valuations used experimental economics, via a mechanism based on direct disclosure of their willingness to pay (maximum purchase price for a bottle of wine according to available information). The results showed that, on a purely sensory level, consumers had difficulty in accepting wine from a resistant variety. We were then able to see that communication focusing on environmental and health performances very much improved the position of the resistant variety of wine, putting it ultimately at the top of the average qualitative evaluations. In economic terms, we show that this promotion results in high market share, gained from conventional wines. Market share losses were lower, however, for the premium conventional wine, suggesting that the higher quality wines would be less directly challenged by wines produced from resistant varieties. © 2018 Université de Bordeaux.
Published: 2018
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36. Research on the origin and the side effects of chitosan stabilizing properties in wine

Author: Dols, Marguerite, Paulin, Margot, Miot-Sertier, Cecile, Claisse, Olivier, Ballestra, Patricia, Albertin, Warren, Marchal, Axel, Masneuf, Isabelle, Delattre, Cédric, Pierre, Guillaume, Dubessay, Pascal, Gardarin, Christine, Michaud, Philippe, Doco, Thierry, Coulon, Joana, Massot, Arnaud, Vallet-Courbin, Amélie, Maupeu, Julie, Unité de Recherche Oenologie [Villenave d'Ornon], Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV), Institut Pascal - Clermont Auvergne (IP), Sigma CLERMONT (Sigma CLERMONT)-Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA), Sciences Pour l'Oenologie (SPO), Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Bio-Laffort, Microflora, Institut des Sciences de la Vigne et du Vin, Institut Pascal (IP), SIGMA Clermont (SIGMA Clermont)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université Montpellier 1 (UM1)-Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA), Unité de Recherche Oenologie [Villenave d'Ornon] (OENO), Université Montpellier 1 (UM1)-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Sigma CLERMONT (Sigma CLERMONT)-Université Clermont Auvergne (UCA)-Centre National de la Recherche Scientifique (CNRS), and Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)
Subjects: [SDV.SA]Life Sciences [q-bio]/Agricultural sciences, Vegetal Biology, vin, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, chitosan, wine, chitosane, Biologie végétale, Sciences agricoles, Agricultural sciences
Abstract: Research on the origin and the side effects of chitosan stabilizing properties in wine. Applied Natural Products : From Discoveries To Innovations
Published: 2018

37. Effects of nutritional state, aging and high chronic intake of sucrose on brain protein synthesis in rats: modulation of it by rutin and other micronutrients

Author: Eva Gatineau, Dominique Dardevet, Didier Rémond, Isabelle Papet, Stéphanie Krisa, Tristan Richard, Sergio Polakof, Laurent Mosoni, Carole Migné, Stéphanie Cluzet, Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), Unité de Recherche Oenologie [Villenave d'Ornon], Institut des Sciences de la Vigne et du Vin (ISVV)-Université de Bordeaux (UB)-Institut National de la Recherche Agronomique (INRA), EA 3675 ISW, Groupe d’Etudes des Substances Végétales à Activité Biologique (GESVAB ), Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV), Unité de Recherche Oenologie [Villenave d'Ornon] (OENO), Unité de Nutrition Humaine - Clermont Auvergne ( UNH ), Université Clermont Auvergne ( UCA ) -Institut national de la recherche agronomique [Auvergne/Rhône-Alpes] ( INRA Auvergne/Rhône-Alpes ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bordeaux ( UB ) -Institut des Sciences de la Vigne et du Vin (ISVV), and Groupe d’Etudes des Substances Végétales à Activité Biologique ( GESVAB )
Subjects: Male, 0301 basic medicine, Aging, Sucrose, Antioxidant, [ SDV.AEN ] Life Sciences [q-bio]/Food and Nutrition, Rutin, medicine.medical_treatment, Interleukin-1beta, Hippocampus, chemistry.chemical_compound, 0302 clinical medicine, Dietary Sucrose, Vitamin E, rat, Micronutrients, Vitamin D, Vitamin A, 2. Zero hunger, chemistry.chemical_classification, biology, Glutathione peroxidase, Brain, General Medicine, vieillissement, Micronutrient, Zinc, Catalase, saccharose, vitamin p, medicine.medical_specialty, NF-E2-Related Factor 2, Selenium, 03 medical and health sciences, Internal medicine, medicine, Animals, Humans, vieillissement cerebral, Rats, Wistar, Interleukin-6, synthèse de protéine, Glutathione, Rats, Vieillissement Cérébral, micronutriment, nutritional status, 030104 developmental biology, Endocrinology, chemistry, Ageing, ageing, Protein Biosynthesis, Dietary Supplements, biology.protein, état nutritionnel, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, 030217 neurology & neurosurgery, rutine, Food Science
Abstract: This work was supported by the Institut National de la Recherche Agronomique (INRA), France. We thank Christophe Delhomme, Philippe Denis, Philippe Lhoste and Arlette Cissoire for animal management and Nordine Hafnaoui for technical assistance; Little is still known about brain protein synthesis. In order to increase our knowledge of it, we aimed to modulate brain protein synthesis rates through aging, variations in nutritional state (fed state vs. fasted state), high sucrose diet and micronutrient supplementation. Four groups of 16 month-old male rats were fed for five months with a diet containing either 13% or 62% sucrose (wheat starch was replaced with sucrose), supplemented or not with rutin (5 g kg-1 diet), vitamin E (4×), A (2×), D (5×), selenium (10×) and zinc (+44%) and compared with an adult control group. We measured cerebellum protein synthesis and hippocampus gene expression of antioxidant enzymes, inflammatory cytokines and transcription factors. We showed that cerebellum protein synthesis was unchanged by the nutritional state, decreased during aging (-8%), and restored to the adult level by micronutrient supplementation. Sucrose diet did not change protein synthesis but reduced the protein content. Micronutrient supplementation had no effect in sucrose fed rats. Hippocampus gene expressions were affected by age (an increase of TNF-α), sucrose treatment (an increase of IL-1β and IL-6), and micronutrient supplementation (a decrease of heme oxygenase, catalase, glutathione peroxidase, TNF-α, and Nrf2). We noted that cerebellum protein synthesis and hippocampus TNF-α gene expression were modulated by the same factors: they were affected by aging and micronutrient supplementation and unchanged by feeding and by high sucrose diet.
Published: 2018
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38. Thoracic skeletal anomalies following surgical treatment of esophageal atresia. Lessons from a national cohort

Author: Nicoleta Panait, E. Habonimana, Philine De Vries, F. Lefebvre, Hubert Lardy, Olivier Jaby, Christian Piolat, Frédéric Auber, Julia Boubnova, Sabine Irtan, Guillaume Podevin, Thierry Lamireau, François Bastard, Aurélie Le Mandat, Aline Ranke-Chrétien, L. Fourcade, Jean-Luc Michel, Jean François Lecompte, Laurent Michaud, Arnaud Bonnard, Françoise Schmitt, Benoit Delorme, Marc Margaryan, Josephine Borgnon, François Becmeur, Anne Dariel, Virginie Fouquet, J. Gaudin, Corinne Borderon, H. Allal, Thomas Gelas, V. Rousseau, Cecilia Tolg, P. Buisson, T. Petit, Unité Pédiatrique [Jeanne de Flandre], Hôpital Jeanne de Flandre [Lille], Service de neurochirurgie pédiatrique [CHU Necker], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Service de chirurgie pédiatrique [CHU Grenoble], Centre Hospitalier Universitaire [Grenoble] (CHU), Service de chirurgie pédiatrique [CHU Strasbourg], Les Hôpitaux Universitaires de Strasbourg (HUS), Unité de Recherche Oenologie [Villenave d'Ornon], Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV), Hôpital des Enfants, CHU Toulouse [Toulouse], Bordeaux population health (BPH), Université de Bordeaux (UB)-Institut de Santé Publique, d'Épidémiologie et de Développement (ISPED)-Institut National de la Santé et de la Recherche Médicale (INSERM), CRLCC Paul Strauss, Hôpital Bicètre, Service de chirurgie pédiatrique, Hôpital Bicêtre, Chirurgie orthopédique et pédiatrique [Hôpital de la Timone - APHM], Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)- Hôpital de la Timone [CHU - APHM] (TIMONE), CHU Pontchaillou [Rennes], Laboratoire de recherche sur la gouvernance publique, territoire et communication (LARGOTEC/CECCOPOP), Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Centre Hospitalier Régional Universitaire de Tours (CHRU de Tours), Service de chirurgie viscérale pédiatrique [CHU Trousseau], CHU Trousseau [APHP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), CHU Clermont-Ferrand, Centre Hospitalier Régional Universitaire de Brest (CHRU Brest), Biomolécules Thérapies anti-tumorales (EA4021), Université de Limoges (UNILIM)-Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST FR CNRS 3503), Hémodynamique, Interaction Fibrose et Invasivité tumorales Hépatiques (HIFIH), Université d'Angers (UA), Centre Hospitalier Universitaire d'Angers (CHU Angers), PRES Université Nantes Angers Le Mans (UNAM), Unité de Recherche Oenologie [Villenave d'Ornon] (OENO), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Trousseau [APHP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU), Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)
Subjects: Male, medicine.medical_specialty, medicine.medical_treatment, Thoracic, [SDV]Life Sciences [q-bio], Scoliosis, 03 medical and health sciences, 0302 clinical medicine, Thoracic Diseases, 030225 pediatrics, medicine, Operative report, Thoracoscopy, Humans, Thoracotomy, Child, Esophageal Atresia, Digestive System Surgical Procedures, Retrospective Studies, Rib cage, medicine.diagnostic_test, business.industry, Retrospective cohort study, General Medicine, medicine.disease, 3. Good health, Surgery, Musculoskeletal Abnormalities, Radiography, medicine.anatomical_structure, Treatment Outcome, Atresia, Pediatrics, Perinatology and Child Health, Radiography, Thoracic, 030211 gastroenterology & hepatology, Female, business, Thoracic wall
Abstract: International audience; INTRODUCTION: Thoracotomy as surgical approach for esophageal atresia treatment entails the risk of deformation of the rib cage and consequently secondary thoracogenic scoliosis. The aim of our study was to assess these thoracic wall anomalies on a large national cohort and search for factors influencing this morbidity.MATERIALS AND METHODS: Pediatric surgery departments from our national network were asked to send recent thoracic X-ray and operative reports for patients born between 2008 and 2010 with esophageal atresia. The X-rays were read in a double-blind manner to detect costal and vertebral anomalies.RESULTS: Among 322 inclusions from 32 centers, 110 (34.2%) X-rays were normal and 25 (7.7%) displayed thoracic malformations, including 14 hemivertebrae. We found 187 (58.1%) sequelae of surgery, including 85 costal hypoplasia, 47 other types of costal anomalies, 46 intercostal space anomalies, 21 costal fusions and 12 scoliosis, with some patients suffering from several lesions. The rate of patients with these sequelae was not influenced by age at intervention, weight at birth, type of atresia, number of thoracotomy or size of the center. The rate of sequelae was higher following a classical thoracotomy (59.1%), whatever the way that thoracotomy was performed, compared to nonconverted thoracoscopy (22.2%; p=0.04).CONCLUSION: About 60 % of the patients suffered from a thoracic wall morbidity caused by the thoracotomy performed as part of surgical treatment of esophageal atresia. Minimally invasive techniques reduced thoracic wall morbidity. Further studies should be carried out to assess the potential benefit of minimally invasive approaches to patient pulmonary functions and on the occurrence of thoracogenic scoliosis in adulthood.LEVELS OF EVIDENCE: Level III retrospective comparative treatment study.
Published: 2018
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39. Reduced obesity, diabetes and steatosis upon cinnamon and grape pomace are associated with changes in gut microbiota and markers of gut barrier

Author: Hubert Plovier, Patrice D. Cani, Amandine Everard, Moez Rhimi, Emmanuelle Maguin, Marcus Ståhlman, Angèle Guilbot, Matthias Van Hul, Lucie Geurts, Pierre-Louis Teissedre, Céline Druart, Philippe Gérard, Fredrik Bäckhed, Amandine Brochot, Kleopatra Chira, Nathalie M. Delzenne, Université Catholique de Louvain = Catholic University of Louvain (UCL), University of Gothenburg (GU), MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Université Paris Saclay (COmUE), Unité de Recherche Oenologie [Villenave d'Ornon], Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV), Groupe PiLeJe, University of Copenhagen = Københavns Universitet (KU), FRFS-WELBIO, WELBIO-CGR-2017, Funds Baillet-Latour grant for medical research 2015, FIRST Spin-Off grant from the Walloon Region, European Project: 336452,EC:FP7:ERC,ERC-2013-StG,ENIGMO(2013), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité de Recherche Oenologie [Villenave d'Ornon] (OENO), University of Copenhagen = Københavns Universitet (UCPH), Microbiota Interaction with Human and Animal (MIHA), Institut National de la Recherche Agronomique (INRA)-AgroParisTech-Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Brochot, Amandine, Gérard, Philippe, Bäckhed, Fredrik, Cani, Patrice D., and UCL - SSS/LDRI - Louvain Drug Research Institute
Subjects: 0301 basic medicine, Male, obesity, Cinnamomum zeylanicum, Physiology, Endocrinology, Diabetes and Metabolism, [SDV]Life Sciences [q-bio], Gut flora, Mice, 0302 clinical medicine, Gut barrier, [SDV.IDA]Life Sciences [q-bio]/Food engineering, Vitis, Intestinal Mucosa, 2. Zero hunger, biology, diabetes, [SDV.BA]Life Sciences [q-bio]/Animal biology, Diabetes, food and beverages, gut barrier, gut microbiota, polyphenols, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, medicine.medical_specialty, 030209 endocrinology & metabolism, Gut microbiota, Diet, High-Fat, Permeability, Diabetes Mellitus, Experimental, 03 medical and health sciences, Metabolic Diseases, Physiology (medical), Internal medicine, Diabetes mellitus, medicine, Animals, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, Obesity, Plant Extracts, Pomace, Polyphenols, biology.organism_classification, medicine.disease, Gastrointestinal Microbiome, Fatty Liver, Mice, Inbred C57BL, 030104 developmental biology, Endocrinology, Steatosis, Metabolic syndrome, Biomarkers
Abstract: International audience; Increasing evidence suggests that polyphenols have a significant potential in the prevention and treatment of risk factors associated with metabolic syndrome. The objective of this study was to assess the metabolic outcomes of two polyphenol-containing extracts from cinnamon bark (CBE) and grape pomace (GPE) on C57BL/6J mice fed a high-fat diet (HFD) for 8 weeks. Both CBE and GPE were able to decrease fat mass gain and adipose tissue inflammation in mice fed a HFD without reducing food intake. This was associated with reduced liver steatosis and lower plasma non-esterified fatty acids levels. We also observed a beneficial effect on glucose homeostasis as evidenced by an improved glucose tolerance and a lower insulin resistance index. These ameliorations of the overall metabolic profile were associated to a significant impact on the microbial composition, which was more profound for the GPE than for the CBE. At the genus level, Peptococcus were decreased in the CBE group. In the GPE treated group, several key genera that have been previously found to be linked with HFD, metabolic effects and gut barrier integrity were affected: we observed a decrease of Desulfovibrio, Lactococcus, whereas Allobaculum and Roseburia were increased. In addition, the expression of several antimicrobial peptides and tight junction proteins was increased in response to both CBE and GPE supplementation, indicating an improvement of the gut barrier function. Collectively, these data suggest that CBE and GPE can ameliorate the overall metabolic profile of mice on a high-fat diet, partly by acting on the gut microbiota.
Published: 2018
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40. A dextran with unique rheological properties produced by the dextransucrase from Oenococcus kitaharae DSM 17330

Author: Marguerite Dols-Lafargue, Claire Moulis, Florent Grimaud, Magali Remaud-Simeon, Pierre Monsan, Catherine Garnier, Patrick Lucas, Marlène Vuillemin, Marion Claverie, Agnès Rolland-Sabaté, Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Recherche Agronomique (INRA), Sécurité et Qualité des Produits d'Origine Végétale (SQPOV), Avignon Université (AU)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de la Recherche Agronomique (INRA), Unité de Recherche Oenologie [Villenave d'Ornon], Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV), Toulouse White Biotechnology (TWB), ANR-10-ALIA-0003,OENOPOLYS,Les polysaccharides de la bactérie lactique Oenococcus oeni, de l'élucidation de leurs structures et voies de biosynthèse à leur valorisation technologique(2010), ANR-14-CE27-0011,ASPIRE,Exploration des relations structure-propriétés de copolymères biosourcés(2014), Institut National de la Recherche Agronomique (INRA)-Avignon Université (AU), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), ANR-10-ALIA-0003, ANR-14-CE27-0011-01, Avignon Université (AU)-Institut National de la Recherche Agronomique (INRA), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), and Unité de Recherche Oenologie [Villenave d'Ornon] (OENO)
Subjects: 0301 basic medicine, Sucrose, Polymers and Plastics, plavolex, Glucansucrase, gène codant, 010402 general chemistry, Polysaccharide, 01 natural sciences, Dextransucrase, sucrose 6-glucosyltransferase, 03 medical and health sciences, chemistry.chemical_compound, Materials Chemistry, Carbohydrate Conformation, spreadability, Enzyme kinetics, Amino Acid Sequence, Databases, Protein, chemistry.chemical_classification, Molar mass, biology, sulfate de dextrane, propriété rhéologique, Chemistry, Viscosity, dextransucrase, Organic Chemistry, Bacterial polysaccharide, oenococcus, Dextrans, biology.organism_classification, Recombinant Proteins, 0104 chemical sciences, 030104 developmental biology, Dextran, [CHIM.POLY]Chemical Sciences/Polymers, Biochemistry, Glucosyltransferases, Oenococcus kitaharae, dextran, polysaccharide, biology.protein, Biocatalysis, saccharase, Oenococcus
Abstract: A gene encoding a novel dextransucrase was identified in the genome of Oenococcus kitaharae DSM17330 and cloned into E. coli . With a kcat of 691 s −1 and a half-life time of 111 h at 30 °C, the resulting recombinant enzyme -named DSR-OK- stands as one of the most efficient and stable dextransucrase characterized to date. From sucrose, this enzyme catalyzes the synthesis of a quasi linear dextran with a molar mass higher than 1 × 10 9 g·mol −1 that presents uncommon rheological properties such as a higher viscosity than that of the most industrially used dextran from L. mesenteroides NRRL-B-512F, a yield stress that was never described before for any type of dextran, as well as a gel-like structure. All these properties open the way to a vast array of new applications in health, food/feed, bulk or fine chemicals fields.
Published: 2018
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41. Lessons from a Prospective on the French Wine Industry Under Climate Change (2050)

Author: Jean-Marc Touzard, Patrick Aigrain, Benjamin Bois, Eric Duchêne, Iñaki Garcia de Cortazar-Atauri, Eric Giraud-Heraud, Jacques Gautier, Hervé Hannin, Nathalie Ollat, Innovation et Développement dans l'Agriculture et l'Alimentation (UMR Innovation), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), France Agrimer, Université de Bourgogne (UB), Santé de la vigne et qualité du vin (SVQV), Institut National de la Recherche Agronomique (INRA)-Université de Strasbourg (UNISTRA), Agroclim (AGROCLIM), Institut National de la Recherche Agronomique (INRA), Unité de Recherche Oenologie [Villenave d'Ornon], Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV), Institut National des Appellations d'Origine (INAO), Marchés, Organisations, Institutions et Stratégies d'Acteurs, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre International de Hautes Etudes Agronomiques Méditerranéennes - Institut Agronomique Méditerranéen de Montpellier (CIHEAM-IAMM), Centre International de Hautes Études Agronomiques Méditerranéennes (CIHEAM)-Centre International de Hautes Études Agronomiques Méditerranéennes (CIHEAM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), IHEV Institut des hautes études de la vigne et du vin, Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Ecophysiologie et Génomique Fonctionnelle de la Vigne (UMR EGFV), Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro), Projet LACCAVE Long term impacts and Adaptations to Climate Change in Viticulture and Enology, Nouveau partenariat pour le développement de l'Afrique (NEPAD). Midrand, ZAF., Unité de Recherche Oenologie [Villenave d'Ornon] (OENO), and Institut des Sciences de la Vigne et du Vin (ISVV)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro)-Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)
Subjects: climate change, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.IDA]Life Sciences [q-bio]/Food engineering, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, adaptation, prospective, wine, ComputingMilieux_MISCELLANEOUS, scenario, [SHS]Humanities and Social Sciences
Abstract: International audience
Published: 2017

42. Molecular determinism of specific anosmia to 2-bromo-4-methylphenol

Author: Tempere, Sophie, Schaaper, Marie-Hélène, LYTRA, Georgia, Barbe, Jean-Christophe, Riquier, Laurent, MARION, Stéphanie, De Revel, Gilles, Sicard, Gilles, Unité de Recherche Oenologie [Villenave d'Ornon], Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV), Neurobiologie des interactions cellulaires et neurophysiopathologie - NICN (NICN), Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Unité de Recherche Oenologie [Villenave d'Ornon] (OENO), and Institut des Sciences de la Vigne et du Vin (ISVV)-Université de Bordeaux (UB)-Institut National de la Recherche Agronomique (INRA)
Subjects: analyse sensorielle, hyposmie, phénotype, detection threshold, oenologie, [SDV]Life Sciences [q-bio], structure-odour relationship, 2-bromo-4-methylphenol, composé olfactif, anosmia, sensory analysis
Abstract: Several studies have revealed considerable variations in human chemosensory capacities. Considerable interindividual differences in sensitivity to various odorants and specific hyposmias are well known. Many chemical studies have focused on elucidating structure-odour relationships on the basis of detection threshold measurements. The basic idea of quantitative structure-activity relationship studies is that a molecule's structure provides information about its biological activity. In a recent sensory assay of 2-bromo-4-methylphenol, Barbe et al. (2014) highlighted a noticeable bimodal distribution of individual sensitivity with large interindividual differences. The aim of the present study was to investigate the link between the chemical structure of 2-bromo-4-methylphenol and this bimodal distribution. The olfactory phenotypes for a selection of six structurally related halogenated compounds were compared using detection thresholds determination. This study revealed that specific anosmia for 2-bromo-4-methylphenol was apparently linked to the halogenated substitution position.
Published: 2017
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43. Réchauffement climatique et acceptabilité des vins par le consommateur

Author: Fuentes Espinoza, Alejandro, Pérès, Stéphanie, Pons, Alexandre, TEMPERE, Sophie, Darriet, Philippe, Giraud-Heraud, Eric, Groupe de Recherche en Economie Théorique et Appliquée (GREThA), Centre National de la Recherche Scientifique (CNRS)-Université de Bordeaux (UB), Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro), Institut des Sciences de la Vigne et du Vin (ISVV), Unité de Recherche Oenologie [Villenave d'Ornon], Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV), Seguin Moreau, Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), Unité de Recherche Oenologie [Villenave d'Ornon] (OENO), and ProdInra, Migration
Subjects: [SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering, Vins de Bordeaux, [SDV.IDA]Life Sciences [q-bio]/Food engineering, Consommateur, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, [SHS] Humanities and Social Sciences, [SDV.IDA] Life Sciences [q-bio]/Food engineering, Fruit confituré, Fruit frais, ComputingMilieux_MISCELLANEOUS, Réchauffement climatique, [SHS]Humanities and Social Sciences
Abstract: Article dans une revue professionnelle
Published: 2016

44. Comment sont appréciés par les consommateurs les vins issus du changement climatique ?

Author: Giraud-Heraud, Eric, Fuentes Espinoza, Alejandro, Pérès, A., Pons, Alexandre, TEMPERE, Sophie, Darriet, Philippe, Unité de Recherche Oenologie [Villenave d'Ornon] (OENO), Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV), Sciences pour l'Action et le Développement : Activités, Produits, Territoires (SADAPT), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro), Seguin Moreau, Institut Français de la Vigne et du Vin (IFV). FRA., and Unité de Recherche Oenologie [Villenave d'Ornon]
Subjects: vitis vinifera, qualité du vin, oenologie, [SDV]Life Sciences [q-bio], [SDV.IDA]Life Sciences [q-bio]/Food engineering, vin, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, adaptation au changement climatique, enquête auprès des consommateurs, ComputingMilieux_MISCELLANEOUS, [SHS]Humanities and Social Sciences
Abstract: National audience
Published: 2016

45. Le vignoble français à l'épreuve du changement climatique

Author: Jean-Louis Escudier, Inaki Garcia de Cortazar Atauri, Eric Giraud-Heraud, Renan Le Roux, Nathalie Ollat, Hervé Quénol, Jean-Marc Touzard, Unité de Recherche Oenologie [Villenave d'Ornon], Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Ecophysiologie et Génomique Fonctionnelle de la Vigne (UMR EGFV), Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité expérimentale de Pech-Rouge (PECH ROUGE), Institut National de la Recherche Agronomique (INRA), Agroclim (AGROCLIM), Unité de Recherche Oenologie [Villenave d'Ornon] (OENO), Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV), Littoral, Environnement, Télédétection, Géomatique (LETG - Rennes), Littoral, Environnement, Télédétection, Géomatique UMR 6554 (LETG), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Université d'Angers (UA)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Brest (UBO)-Université de Rennes 2 (UR2), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS)-Institut de Géographie et d'Aménagement Régional de l'Université de Nantes (IGARUN), Université de Nantes (UN)-Université de Nantes (UN)-Université de Caen Normandie (UNICAEN), Université de Nantes (UN)-Université de Nantes (UN), Institut des Sciences de la Vigne et du Vin (ISVV)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro)-Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB), Innovation et Développement dans l'Agriculture et l'Alimentation (UMR Innovation), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), ProdInra, Migration, Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)
Subjects: [SDE] Environmental Sciences, Changement climatique, Viticulture, [SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering, [SDV]Life Sciences [q-bio], [SDV.IDA] Life Sciences [q-bio]/Food engineering, Résistance, résistanve, [SHS]Humanities and Social Sciences, [SDV] Life Sciences [q-bio], [SDV.IDA]Life Sciences [q-bio]/Food engineering, [SDE]Environmental Sciences, Vin, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, Vigne, [SHS] Humanities and Social Sciences, ComputingMilieux_MISCELLANEOUS
Abstract: National audience
Published: 2016

46. Two new stilbenoids from the aerial parts of Arundina graminifolia (Orchidaceae)

Author: Frederic Bonte, Annelise Lobstein, Gaëtan Herbette, Cyril Antheaume, Opeyemi Joshua Olatunji, Jean-Michel Mérillon, Stéphanie Krisa, Florence Auberon, Laboratory of Pharmacognosy and Bioactive Natural Products, Faculty of Pharmacy, Université de Strasbourg (UNISTRA), Unité de Recherche Oenologie [Villenave d'Ornon], Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV), Groupe d’Etudes des Substances Végétales à Activité Biologique (GESVAB ), Institut des Sciences de la Vigne et du Vin, Laboratoire Insulaire du Vivant et de l'Environnement (LIVE), Université de la Nouvelle-Calédonie (UNC), Spectropôle - Aix Marseille Université (AMU SPEC), Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), LVMH Moët Hennessy Louis Vuitton, Laboratoire Insulaire du Vivant et de l’Environnement, EA 4243, Université de la Nouvelle Calédonie (UNC), USC 1366 Oenologie, Institut National de la Recherche Agronomique (INRA), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), and Unité de Recherche Oenologie [Villenave d'Ornon] (OENO)
Subjects: phenanthrene, Stereochemistry, Cell Survival, Dimer, [SDV]Life Sciences [q-bio], Pharmaceutical Science, arundiquinone, 01 natural sciences, PC12 Cells, cytoprotecteur, Article, spectroscopie, Analytical Chemistry, lcsh:QD241-441, chemistry.chemical_compound, lcsh:Organic chemistry, Arundina, Drug Discovery, Stilbenes, Lignin, Organic chemistry, Animals, ornamental orchids, arundigramin, Physical and Theoretical Chemistry, Orchidaceae, Amyloid beta-Peptides, biology, 010405 organic chemistry, Chemistry, Organic Chemistry, orchidaceae, Arundina graminifolia, Phenanthrene, Plant Components, Aerial, phenanthrene derivatives, biology.organism_classification, cytoprotector, 0104 chemical sciences, Rats, 010404 medicinal & biomolecular chemistry, Heteronuclear molecule, Chemistry (miscellaneous), Cytoprotection, Molecular Medicine, Two-dimensional nuclear magnetic resonance spectroscopy, Heteronuclear single quantum coherence spectroscopy
Abstract: Two new phenanthrene derivatives, a phenanthrenequinone named arundiquinone (1) and a 9,10-dihydrophenanthrene named arundigramin (2) together with a known lignin dimer (3) and seven known stilbenoids (4-10) were isolated from the aerial parts of the Asian orchid Arundina graminifolia. The structures of the isolated compounds were elucidated by spectroscopic methods, including extensive 1D, 2D NMR (heteronuclear single quantum coherence (HSQC), heteronuclear multiple-bond correlation spectroscopy (HMBC), and HR-ESI-MS techniques, as well as comparison with respective literature reports. The cytoprotective activity of the isolated compounds were evaluated for their ability to reduce beta amyloid induced toxicity on undifferentiated PC12 cells. Compound 8 showed moderate cytoprotective activity at 0.5 µmol/L (71% of cell viability) while the other compounds showed no significant activity at the highest concentration tested.
Published: 2016
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47. Phylogenomic Analysis of Oenococcus oeni Reveals Specific Domestication of Strains to Cider and Wines

Author: Campbell-Sills, Hugo, El Khoury, Mariette, Favier, Marion, Romano, Andrea, Biasioli, Franco, Spano, Giuseppe, Sherman, David James, Bouchez, Olivier, Coton, Emmanuel, Coton, Monika, Okada, Sanae, Tanaka, Naoto, Dols-Lafargue, Marguerite, Lucas, Patrick M, Unité de Recherche Oenologie [Villenave d'Ornon], Institut des Sciences de la Vigne et du Vin (ISVV)-Université de Bordeaux (UB)-Institut National de la Recherche Agronomique (INRA), Fondazione Edmund Mach - Functional genomics, Fondazione Edmund Mach - Edmund Mach Foundation [Italie] (FEM), Laffort [Floirac], Dipartimento di Scienze Agrarie, degli Alimenti e dell'Ambiente - Department of the Sciences of Agriculture, Food and Environment [University of Foggia], Università degli Studi di Foggia - University of Foggia, from patterns to models in computational biodiversity and biotechnology (PLEIADE), Biodiversité, Gènes & Communautés (BioGeCo), Université de Bordeaux (UB)-Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut National de la Recherche Agronomique (INRA)-Inria Bordeaux - Sud-Ouest, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-Laboratoire Bordelais de Recherche en Informatique (LaBRI), Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2, Laboratoire Bordelais de Recherche en Informatique (LaBRI), Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2, GeT PlaGe, Genotoul, Institut National de la Recherche Agronomique (INRA), Laboratoire de Génétique Cellulaire (LGC), Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA), Laboratoire Universitaire de Biodiversité et Ecologie Microbienne (LUBEM), Université de Brest (UBO), Tokyo University of Agriculture and Technology (TUAT), Institut Polytechnique de Bordeaux (Bordeaux INP), Conseil Régional de l'Aquitaine SAGESSE, European Project: 315065,EC:FP7:SME,FP7-SME-2012,WILDWINE(2012), Unité de Recherche Oenologie [Villenave d'Ornon] (OENO), Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV), Università degli Studi di Foggia = University of Foggia (Unifg), Université de Bordeaux (UB)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Centre National de la Recherche Scientifique (CNRS)-Université de Bordeaux (UB)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Centre National de la Recherche Scientifique (CNRS)-Inria Bordeaux - Sud-Ouest, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-Biodiversité, Gènes & Communautés (BioGeCo), Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut National de la Recherche Agronomique (INRA), Université de Bordeaux (UB)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Centre National de la Recherche Scientifique (CNRS), Génome et Transcriptome - Plateforme Génomique (GeT-PlaGe), Institut National de la Recherche Agronomique (INRA)-Plateforme Génome & Transcriptome (GET), Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université de Toulouse (UT)-Université de Toulouse (UT), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Inria Bordeaux - Sud-Ouest, Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3), Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Inria Bordeaux - Sud-Ouest, and Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)
Subjects: Oenococcus oeni, domestication, genomics, phylogeny, population structure, polymorphisme des nucléotides simples, phylogénomique, food and beverages, Wine, champagne, Beverages, Evolution, Molecular, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], vin, cidre, fermentation malolactique, [INFO.INFO-BI]Computer Science [cs]/Bioinformatics [q-bio.QM], typage moléculaire, Genome, Bacterial, Oenococcus, Research Article
Abstract: International audience; Oenococcus oeni is a lactic acid bacteria species encountered particularly in wine, where it achieves the malolactic fermentation. Molecular typing methods have previously revealed that the species is made of several genetic groups of strains, some being specific to certain types of wines, ciders or regions. Here, we describe 36 recently released O. oeni genomes and the phylogenomic analysis of these 36 plus 14 previously reported genomes. We also report three genome sequences of the sister species Oenococcus kitaharae that were used for phylogenomic reconstructions. Phylogenomic and population structure analyses performed revealed that the 50 O. oeni genomes delineate two major groups of 12 and 37 strains, respectively, named A and B, plus a putative group C, consisting of a single strain. A study on the orthologs and single nucleotide polymorphism contents of the genetic groups revealed that the domestication of some strains to products such as cider, wine, or champagne, is reflected at the genetic level. While group A strains proved to be predominant in wine and to form subgroups adapted to specific types of wine such as champagne, group B strains were found in wine and cider. The strain from putative group C was isolated from cider and genetically closer to group B strains. The results suggest that ancestral O. oeni strains were adapted to low-ethanol containing environments such as overripe fruits, and that they were domesticated to cider and wine, with group A strains being naturally selected in a process of further domestication to specific wines such as champagne.
Published: 2015
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48. Consentements à payer relatifs et mécanisme de comparaison des surplus dans les enchères expérimentales

Author: Combris, Pierre, Giraud-Heraud, Eric, Seabra Pinto, Alexandra, Alimentation et sciences sociales (ALISS), Institut National de la Recherche Agronomique (INRA), Unité de Recherche Oenologie [Villenave d'Ornon], Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV), Groupe de Recherche en Economie Théorique et Appliquée (GREThA), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), National Institute for Agrarian and Veterinarian Research I.P., and Unité de Recherche Oenologie [Villenave d'Ornon] (OENO)
Subjects: jel:Q51, jel:C91, [SDV.IDA]Life Sciences [q-bio]/Food engineering, jel:D44, Experimental Auctions, Willingness to pay, Consumers’ surplus, Choice alternatives, Food certification, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, [SHS]Humanities and Social Sciences
Abstract: We study the relative willingness-to-pay (WTP) of consumers according to the diversity of supply in a market and we show how the presence of substitutes for a given product leads to question the incentive mechanisms commonly used in experimental auctions. We propose a Surplus Comparison Mechanism (SCM) in order to yield WTP estimates which better take into account the choice set available to consumers. After showing the efficiency of this mechanism we test the SCM in a laboratory experiment, reconsidering WTP for food environmental certifications (Integrated Pest Management and Organic certification). It appears that WTPs are decreasing when more alternative certifications are offered to consumers., Nous étudions le caractère relatif du consentement à payer (CAP) des consommateurs en vérifiant que celui-ci peut être fonction des alternatives de choix de consommation et de la diversité de l’offre sur un marché. Nous proposons un ‘Mécanisme de Comparaison des Surplus’ pour mieux tenir compte de ces effets de diversité dans les enchères expérimentales. Après avoir démontré l’efficacité de ce mécanisme pour la révélation des préférences nous le testons par une expérience en laboratoire, en reconsidérant la mesure du CAP pour les certifications environnementales des produits alimentaires (Production intégrée et certification biologique). Il apparaît notamment que le CAP est décroissant en fonction du nombre d’alternatives de choix des consommateurs.
Published: 2015

49. Two different Oenococcus oeni lineages are associated to either red or white wines in Burgundy: genomics and metabolomics insights

Author: Lucie Dutilh, Giuseppe Spano, Christophe Hubert, David James Sherman, Gilles de Revel, Marine Gammacurta, Jochen Vestner, Olivier Claisse, Patrick Lucas, Mariette El Khoury, Hugo Campbell-Sills, Vittorio Capozzi, Cécile Miot-Sertier, Unité de Recherche Oenologie [Villenave d'Ornon], Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV), Dipartimento di Scienze Agrarie, degli Alimenti e dell'Ambiente - Department of the Sciences of Agriculture, Food and Environment [University of Foggia], Università degli Studi di Foggia - University of Foggia, Laboratoire Bordelais de Recherche en Informatique (LaBRI), Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2, from patterns to models in computational biodiversity and biotechnology (PLEIADE), Biodiversité, Gènes & Communautés (BioGeCo), Université de Bordeaux (UB)-Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut National de la Recherche Agronomique (INRA)-Inria Bordeaux - Sud-Ouest, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-Laboratoire Bordelais de Recherche en Informatique (LaBRI), Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2, Laboratoire Hubert Curien [Saint Etienne] (LHC), Institut d'Optique Graduate School (IOGS)-Université Jean Monnet [Saint-Étienne] (UJM)-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences de la Vigne et du Vin (ISVV), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, French Ministry of Agriculture CASDAR LevainsBio 2012-1220Conseil Régional Aquitaine 20030304002FAConseil Régional Aquitaine 20040305003FA, ANR-10-EQPX-16-01/10-EQPX-0016,XYLOFOREST,Plateforme d’Innovation « Forêt-Bois-Fibre-Biomasse du Futur »(2010), European Project: 315065,EC:FP7:SME,FP7-SME-2012,WILDWINE(2012), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Inria Bordeaux - Sud-Ouest, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-Biodiversité, Gènes & Communautés (BioGeCo), Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut National de la Recherche Agronomique (INRA), Université Sciences et Technologies - Bordeaux 1-Institut National de la Recherche Agronomique (INRA)-Université Bordeaux Segalen - Bordeaux 2, ANR-10-EQPX-0016,XYLOFOREST,Plateforme d'Innovation ' Forêt-Bois-Fibre-Biomasse du Futur '(2010), Unité de Recherche Oenologie [Villenave d'Ornon] (OENO), Università degli Studi di Foggia = University of Foggia (Unifg), Université de Bordeaux (UB)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Centre National de la Recherche Scientifique (CNRS), Université de Bordeaux (UB)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Centre National de la Recherche Scientifique (CNRS)-Université de Bordeaux (UB)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Centre National de la Recherche Scientifique (CNRS)-Inria Bordeaux - Sud-Ouest, Laboratoire Hubert Curien (LHC), Institut d'Optique Graduate School (IOGS)-Université Jean Monnet - Saint-Étienne (UJM)-Centre National de la Recherche Scientifique (CNRS), and Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1 (UB)
Subjects: 0301 basic medicine, 030106 microbiology, comparative genomics, Horticulture, lcsh:Agriculture, 03 medical and health sciences, chemistry.chemical_compound, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], vin rouge, lcsh:Botany, Botany, séquençage, Malolactic fermentation, Oenococcus oeni, malolactic fermentation, metabolomics, Food science, fermentation malolactique, Wine, biology, génomique comparative, digestive, oral, and skin physiology, lcsh:S, food and beverages, biology.organism_classification, acidité du vin, Lactic acid, lcsh:QK1-989, White (mutation), chemistry, White Wine, Fermentation, Malic acid, Food Science
Abstract: Oenococcus oeni is the bacterium most often associated with spontaneous malolactic fermentation (MLF) of wine. During MLF, malic acid is transformed into lactic acid and several metabolites are modified, modulating wine’s total acidity and improving its sensory properties. Previous works have suggested that certain genetic groups of O. oeni strains are associated to different kinds of products. In the present study we have spotted two groups of strains isolated mainly from Burgundy wines, one associated to red wines and the other to white wines. Sequencing 14 genomes of red and white wine strains revealed that they share a common ancestor that probably colonised two different substrates –red and white wine-associated environments–, diverging over time and disseminating to various regions. Their capacity to perform MLF and modify the volatile profile of wine was determined by fermenting a chardonnay wine and analysing its volatile fraction with a non-targeted metabolomics approach by GC-MS. The strains had a different impact on the volatile composition depending on their group of origin. These results show for the first time a correspondence between the product of origin of the strains and the volatile profile of the wines they produce. Furthermore, the genetic features that might be implied in these different phenotypes are examined.
Published: 2017
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50. Polyphenols from the stems of Morus alba and their inhibitory activity against nitric oxide production by lipopolysaccharide-activated microglia

Author: Laurent Pechamat, Jean-Claude Delaunay, Axel Marchal, Merian Nassra, Pierre Waffo-Teguo, Céline Rivière, Jean-Michel Mérillon, Stéphanie Krisa, Alain Badoc, Institut Charles Viollette (ICV) - EA 7394 (ICV), Université d'Artois (UA)-Institut National de la Recherche Agronomique (INRA)-Université du Littoral Côte d'Opale (ULCO)-Institut Supérieur d'Agriculture-Université de Lille, Institut des Sciences de la Vigne et du Vin (ISVV), Unité de Recherche Oenologie [Villenave d'Ornon] (OENO), Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV), Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Institut National de la Recherche Agronomique (INRA)-Université d'Artois (UA)-Institut Supérieur d'Agriculture, and Unité de Recherche Oenologie [Villenave d'Ornon]
Subjects: Lipopolysaccharides, Dihydroflavonols, Lipopolysaccharide, Biology, Nitric Oxide, Moraceae, 01 natural sciences, Nitric oxide, Cell Line, chemistry.chemical_compound, Mice, Coumarins, Stilbenes, [SDV.IDA]Life Sciences [q-bio]/Food engineering, Drug Discovery, medicine, Animals, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, MTT assay, Glycosides, Inflammation, Pharmacology, chemistry.chemical_classification, Microglia, Molecular Structure, Plant Stems, 010405 organic chemistry, Glycoside, Polyphenols, General Medicine, Coumarin, biology.organism_classification, Morus alba, 0104 chemical sciences, 3. Good health, 010404 medicinal & biomolecular chemistry, medicine.anatomical_structure, chemistry, Biochemistry, Polyphenol, Flavanones, Morus
Abstract: International audience; Neuroinflammatory processes are involved in the pathogenesis of many neurodegenerative disorders. Microglial cells, the main immune cells of the central nervous system, represent a target of interest to search for naturally occurring anti-inflammatory products. In this study, we evaluated the anti-inflammatory properties of polyphenols obtained from the stems of Morus alba. This edible species, known as white mulberry, is frequently studied because of its traditional use in Asian medicine and its richness in different types of polyphenols, some of which are known to be phytoalexins. One new coumarin glycoside, isoscopoletin 6-(6-O-β-apiofuranosyl-β-glucopyranoside) (1) was mainly isolated by CPC (centrifugal partition chromatography) from this plant, together with seven known polyphenols (2-8). Their structures were established on the basis of spectroscopic analyses including extensive 2D NMR studies. The eight isolated compounds were evaluated for their inhibitory activities on nitric oxide (NO) production in lipopolysaccharide (LPS)-induced BV-2 microglial cells. The absence of cell toxicity is checked by a MTT assay.
Published: 2014
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