19 results on '"Unal, Yilmaz"'
Search Results
2. Advanced electrocoagulation using aluminum electrodes supported by persulfate for the treatment of a high-strength wastewater
- Author
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Yokus, Senem, Bilgin, Oyku Nur, Unal Yilmaz, Ezgi, Gundogdu, Hilal, Yazici Guvenc, Senem, Can-Güven, Emine, and Varank, Gamze
- Published
- 2023
- Full Text
- View/download PDF
3. Effective treatment of textile industry wastewater by combined ultraviolet assisted and Fe2+ catalyzed percarbonate oxidation.
- Author
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Unal Yilmaz, Ezgi, Gundogdu, Hilal, Bilgin, Oyku Nur, Yokus, Senem, Turk, Oruc Kaan, Yazici Guvenc, Senem, Can-Güven, Emine, and Varank, Gamze
- Subjects
- *
TEXTILE industry , *SEWAGE , *SUSTAINABILITY , *ULTRAVIOLET lamps , *OXIDATION , *PRECIPITATION scavenging , *COLOR removal (Sewage purification) - Abstract
Sustainable water management is very important for industries with high water consumption and wastewater generation, such as the textile industry. This study aims to investigate the treatment of textile wastewater with ultraviolet (UV) assisted and Fe2+ catalyzed sodium percarbonate (SPC) oxidation, which is an eco-friendly treatment method in terms of both added oxidant and activation methods. Based on the removal efficiencies and reaction rates, a sorting was made as UV/SPC/Fe2+ > SPC/Fe2+ > UV/Fe2+ > UV/SPC > SPC > UV. It was observed that the removal efficiencies increased as the SPC dose increased, and decreased due to the scavenging effect with the 5 g/L oxidant dose, and the optimum SPC dose was determined as 2.5 g/L. It was determined that the removal efficiencies were higher in acidic conditions and high UV power applications. Under optimum operating conditions (pH:3, SPC dose: 2.5 g/L, Fe2+ dose: 1 g/L, UV lamps: 4), 68% COD, 80.9% UV254, and 92% color removal were achieved. Under optimum conditions energy cost, chemical cost, and operational cost were calculated as 11.9 $/m3, 0.20 $/m3, and 12.1 $/m3, respectively. By reducing the process COD concentration to 236.2 mg/L, discharge standards have been achieved, and the applicability of the UV/SPC/Fe2+ process was proven. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
4. Advanced electrocoagulation using aluminum electrodes supported by persulfate for the treatment of a high-strength wastewater
- Author
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Senem Yokus, Oyku Nur Bilgin, Ezgi Unal Yilmaz, Hilal Gundogdu, Senem Yazici Guvenc, Emine Can-Güven, and Gamze Varank
- Subjects
General Chemical Engineering ,General Chemistry - Published
- 2023
5. Minimizing Capacitance Value of Interleaved Power Factor Corrected Boost Converter for Battery Charger in Electric Vehicles
- Author
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Omer Turksoy, Unal Yilmaz, and Ahmet Teke
- Subjects
dynamic voltage compensator ,electric vehicles ,on-board battery chargers ,power factor correction ,Electrical engineering. Electronics. Nuclear engineering ,TK1-9971 - Abstract
A dynamic voltage compensator (DVC) technique is presented for the minimizing of the capacitance value of the interleaved power factor corrected (PFC) boost converter for the battery charger in electric vehicles. This technique is based on eliminating the ripple on the capacitor by creating a voltage in the opposite direction as well as the amount of ripple on the capacitor. With the proposed method, the capacitance value is reduced by approximately five times. Reducing the size of the capacitor also provides the use of film-capacitors with a longer life. The other contribution of this study is designing a faster and more stable fully-digital control system, instead of the commonly used analogue controller of interleaved PFC boost converter. A 3.3 kW interleaved PFC boost converter is designed to verify the effect of the designed dynamic voltage compensator and digital controller.
- Published
- 2019
- Full Text
- View/download PDF
6. Manakov model of coupled NLS equation and its optical soliton solutions
- Author
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Esra Unal Yilmaz, Farid Samsami Khodad, Yesim Saglam Ozkan, Reza Abazari, A.E. Abouelregal, Mayssam Tarighi Shaayesteh, Hadi Rezazadeh, and Hijaz Ahmad
- Subjects
Environmental Engineering ,Ocean Engineering ,Oceanography - Published
- 2022
7. Efficient AC-DC power factor corrected boost converter design for battery charger in electric vehicles
- Author
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Ahmet Teke, Omer Turksoy, Unal Yilmaz, Mühendislik ve Doğa Bilimleri Fakültesi -- Elektrik-Elektronik Mühendisliği Bölümü, and Türksoy, Ömer
- Subjects
Automotive batteries ,Electric vehicles ,Computer science ,Steady-state equilibrium ,02 engineering and technology ,Power factor ,Power factor correction ,Efficiency ,Electric vehicle ,Industrial and Manufacturing Engineering ,Electric inverters ,Harmonic analysis ,Battery charger ,0202 electrical engineering, electronic engineering, information engineering ,Pulse Duration Modulation ,Electronic circuit ,Total harmonic distortion ,Total harmonic distortion (THD) ,DC-DC converters ,Fuel cell ,Electrical engineering ,European efficiencies ,Pollution ,Soft switching ,Rectifying circuits ,General Energy ,Thermodynamics ,Design ,Energy & Fuels ,Rectifier ,020209 energy ,Zero current transition(ZCT) ,Regulatory compliance ,020401 chemical engineering ,Snubber ,Zero Current Switching ,0204 chemical engineering ,Electrical and Electronic Engineering ,Civil and Structural Engineering ,Research work ,Electric power factor correction ,business.industry ,Mechanical Engineering ,Soft-switching technique ,Building and Construction ,Steady-state operating ,Energy efficiency ,Efficiency improvement ,Charging (batteries) ,Boost converter ,Switching ,Buck Converter ,Zero voltage transition ,AC-DC converter ,business ,Electric Vehicles (EVs) ,Voltage - Abstract
With the widespread use of electric vehicles (EVs), the design of a highly quality and efficient battery charger has become an important research topic. In this study, an efficient AC-DC power factor corrected (PFC) boost converter with active snubber cell (ASC) is proposed for the battery charger. ASC integrated into the AC-DC PFC boost converter turns on the main switch with zero voltage transition (ZVT) and turns off with zero current transition (ZCT), which among soft switching techniques for eliminating the switching losses. In addition, it has been provided that other switching elements are operated with soft switching. Soft switching conditions and unity power factor (PF) are achieved from light load to full load conditions. Unlike the soft-switched PFC converter circuits in the current literature, no current and voltage stresses have been observed on any component in the main converter and ASC circuits. The paper also presents the steady-state operating principles and design guidelines of the proposed converter in detail. The efficiency improvement between the hard switched counterpart has been verified by making detailed loss analysis. The proposed converter has been constructed as 3.3 kW and tested according to European efficiency standards with various case studies. The total harmonic distortion (THD) of input current has been measured as less than 5% in compliance with EN 6100-3-2 Class D Limits (A) standards.
- Published
- 2021
8. PV system fuzzy logic MPPT method and PI control as a charge controller
- Author
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Selim Borekci, Unal Yilmaz, and Ali Kircay
- Subjects
Engineering ,Renewable Energy, Sustainability and the Environment ,business.industry ,Buck converter ,020209 energy ,Photovoltaic system ,02 engineering and technology ,Maximum power point tracking ,Charge controller ,Control theory ,Boost converter ,0202 electrical engineering, electronic engineering, information engineering ,Constant current ,Voltage source ,business ,Voltage - Abstract
This paper puts forward to Fuzzy Logic MPPT (Maximum Power Point Tracking) method applied photovoltaic panel sourced boost converter, under variable temperature (25–60 °C) and irradiance (700–1000 W/m2) after that the PI control was applied buck converter to behave as a charge controller. The voltage and current of PV panels are nonlinear and they depend on environmental conditions such as temperature and irradiance. Variable environmental conditions cause to change voltage, current and also cause to change maximum available power of PV panels. To increase efficiency and decrease payback period of the system, it needs to operate PV panels at maximum power point (MPP). Under any environment conditions there is unique MPP. To operate PV panels at that point (MPP) there are many MPPT method in literature, FLC MPPT method was preferred in this study because, its rapid response to changing environmental conditions and not affecting by change of circuit parameters. The accuracy of FLC MPPT method used in this system to find MPP changes, from 94.8% to 99.4%. To charge a battery there are two traditional methods which are constant current (CC), and constant voltage (CV) methods. For fast charging with low loss constant current and voltage source is a need. One of the methods providing constant is PI control which used in this study. PI control is not only well developed and a simple technique but also it provides satisfactory results. The goal of this study is operating PV panel at maximum power point under variable environment conditions to increase efficiency and reduce cost and also provide appropriate current and voltage for charging battery to charge quickly, reduce losses and also increase life cycle of battery. This system was established and analyzed in MATLAB/Simulink.
- Published
- 2018
9. A Comparison Study of Sinusoidal PWM and Space Vector PWM Techniques for Voltage Source Inverter
- Author
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Unal Yilmaz, Omer Turksoy, Ahmet Teke, and Adnan Tan
- Subjects
Engineering, Electrical and Electronic ,020209 energy ,Topology (electrical circuits) ,Mühendislik, Elektrik ve Elektronik ,02 engineering and technology ,Topology ,Space vector PWM ,MATLAB/Simulink ,0202 electrical engineering, electronic engineering, information engineering ,Voltage source ,lcsh:Science (General) ,General Environmental Science ,Physics ,Sinusoidal PWM ,Total harmonic distortion ,020208 electrical & electronic engineering ,lcsh:TA1-2040 ,Modulation ,Harmonic ,General Earth and Planetary Sciences ,Inverter ,total harmonic distortion ,Space vector PWM,Sinusoidal PWM,total harmonic distortion,MATLAB/Simulink ,lcsh:Engineering (General). Civil engineering (General) ,Pulse-width modulation ,lcsh:Q1-390 ,Voltage - Abstract
In this paper, the methods used to control voltage source inverters, which have been intensively investigated in recent years, are compared. Although the most efficient result is obtained with the least number of switching elements in the inverter topologies, the method used in the switching is at least as effective as the topology. Besides, the selected switching method to control the inverter will play an effective role in suppressing harmonic components while producing the ideal output voltage. There are many derivatives of pulse width modulation techniques that are commonly used to control voltage source inverters. Some of widespread methods are sinusoidal pulse width modulation and space vector pulse width modulation techniques. These modulation techniques used for generating variable frequency and amplitude output voltage in voltage source inverters, have been simulated by using MATLAB/SIMULINK. In addition, the total harmonic distortions of the output voltages are compared. As a result of simulation studies, sinusoidal pulse width modulation has been found to have more total harmonic distortion in output voltages of voltage source inverters in the simulation. Space vector pulse width modulation has been shown to produce a more efficient output voltage with less total harmonic distortion.
- Published
- 2017
10. Intelligent control of high energy efficient two-stage battery charger topology for electric vehicles
- Author
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Omer Turksoy, Ahmet Teke, Unal Yilmaz, Çukurova Üniversitesi, Mühendislik ve Doğa Bilimleri Fakültesi -- Elektrik-Elektronik Mühendisliği Bölümü, and Türksoy, Ömer
- Subjects
Artificial intelligence ,Electric vehicles ,Computer science ,02 engineering and technology ,Power factor ,Power factor correction ,Topology ,Industrial and Manufacturing Engineering ,Zero Voltage Switching | DC-DC Converter | Charging (Batteries) ,Harmonic analysis ,Battery charger ,Performance assessment ,Pev charger ,0202 electrical engineering, electronic engineering, information engineering ,Total harmonic distortion ,Total harmonic distortion (THD) ,Fuel cell ,European efficiencies ,Secondary batteries ,Pollution ,Battery chargers ,Rectifying circuits ,General Energy ,Harmonics ,Thermodynamics ,Factor analysis ,Electrical quality ,Neural networks ,Efficient energy use ,Battery (electricity) ,Intelligent control ,Artificial neural network ,Energy & Fuels ,020209 energy ,Dynamic loads ,DC-DC converter ,Topology (electrical circuits) ,020401 chemical engineering ,0204 chemical engineering ,Electrical and Electronic Engineering ,Civil and Structural Engineering ,Mechanical Engineering ,Power factor corrections ,Building and Construction ,Energy efficiency ,LLC resonant converter ,Charging (batteries) ,High energy efficiency ,Electric Vehicles (EVs) ,Voltage - Abstract
Turksoy, Omer/0000-0003-4620-4503, WOS: 000492797300026, The energy efficiency of the battery charging system directly affects the distance which electric vehicles can get with per charge process. In addition, reducing current harmonics distortion (THD) increases the electrical quality and power conversion performance. This paper proposes intelligent control of high-efficiency two-stage battery charger topology for electric vehicles (EVs). In the first stage, for low harmonics of input current, high power factor and high efficiency, the average current mode controlled ac/dc boost power factor (pf) correction method constructed and analyzed with 98% efficiency and the power factor is achieved higher than 0.99. Additionally, in the second stage, for high energy efficiency, higher battery lifespan and allowing EVs to get more miles with per charge, a half-bridge LLC resonant converter controlled with a designed artificial neural network (ANN). Moreover, the LLC resonant converter controlled under dynamic load to analysis system from no-load to full-load. A 3.1 kW powered system has been designed for this study, the efficiency of the system has been calculated based on European efficiency standards and the peak efficiency of the whole system is 96.2%, the load voltage ripple is less than 0.5 V and also total harmonic distortion of the source current is less than 5%. (C) 2019 Elsevier Ltd. All rights reserved.
- Published
- 2019
11. Minimizing capacitance value of interleaved power factor corrected boost converter for battery charger in electric vehicles
- Author
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Ahmet Teke, Unal Yilmaz, Omer Turksoy, Çukurova Üniversitesi, Mühendislik ve Doğa Bilimleri Fakültesi -- Elektrik-Elektronik Mühendisliği Bölümü, and Türksoy, Ömer
- Subjects
PFC ,Electric vehicles ,Computer science ,Dynamic voltage compensator ,020209 energy ,Ripple ,02 engineering and technology ,Power factor ,Hardware_PERFORMANCEANDRELIABILITY ,Power factor correction ,Capacitance ,Electric power factor correction | Rectifying circuits | Conduction losses ,law.invention ,Battery charger ,Engineering ,Control theory ,law ,Hardware_GENERAL ,0202 electrical engineering, electronic engineering, information engineering ,Hardware_INTEGRATEDCIRCUITS ,On-board battery chargers ,Electrical and Electronic Engineering ,business.industry ,Electrical engineering ,021001 nanoscience & nanotechnology ,Capacitor ,Boost converter ,Electrical & Electronic ,lcsh:Electrical engineering. Electronics. Nuclear engineering ,0210 nano-technology ,business ,lcsh:TK1-9971 ,Voltage - Abstract
WOS: 000490998600002, A dynamic voltage compensator (DVC) technique is presented for the minimizing of the capacitance value of the interleaved power factor corrected (PFC) boost converter for the battery charger in electric vehicles. This technique is based on eliminating the ripple on the capacitor by creating a voltage in the opposite direction as well as the amount of ripple on the capacitor. With the proposed method, the capacitance value is reduced by approximately five times. Reducing the size of the capacitor also provides the use of film-capacitors with a longer life. The other contribution of this study is designing a faster and more stable fully-digital control system, instead of the commonly used analogue controller of interleaved PFC boost converter. A 3.3 kW interleaved PFC boost converter is designed to verify the effect of the designed dynamic voltage compensator and digital controller., Scientific Research Project Unit of Cukurova UniversityCukurova University [FDK-2018-11231], This research study is supported by the Scientific Research Project Unit of Cukurova University with the project number of FDK-2018-11231.
- Published
- 2019
12. PV system flyback converter controlled PI control to charge battery under variable temperature and irradiance
- Author
-
Selim Borekci, Unal Yilmaz, and Ali Kircay
- Subjects
Forward converter ,Materials science ,Flyback converter ,Buck converter ,business.industry ,020209 energy ,Photovoltaic system ,Flyback transformer ,Buck–boost converter ,Electrical engineering ,02 engineering and technology ,021001 nanoscience & nanotechnology ,Flyback diode ,Maximum power point tracking ,Control theory ,0202 electrical engineering, electronic engineering, information engineering ,0210 nano-technology ,business - Abstract
This paper proposes to Flyback converter sourced photovoltaic (PV) Panel is controlled by PI technology, under variable irradiance (800 W/m2 to 1000 w/m2) and temperature (25 °C to 40 °C). The system was used to charge Lithium ion battery for PV panel applications. Voltage and power of PV panels are nonlinear they depend on environment conditions like irradiation and temperature. PV system can be thought as a DC (direct current) source so that one of the DC-DC converters, flyback converter, is used to regulate voltage and current of the PV panel. Flyback converters provide insulation between load and source also by adjustable turn ratio of transformer the load voltage can be regulated. PI control applied to switch (MOSFET) of flyback converter to regulate duty cycle of PWM (Pulse Width Modulation), to charge battery with constant voltage method. The maximum power point of PV module which used in this system, under STC (1000 W/m2 and 25 °C), is around 50 W and the load power of the flyback converter in that condition is around 40 W. The efficiency of system under STC is around 80 %, but when irradiation level decreases to 800 W/m2 and the temperature step up to 40 °C the efficiency of system increases (>95 %). The system was analyzed and constructed in Matlab1Simulink.
- Published
- 2017
13. Does inguinal hernia repair affect uroflowmetric parameters? A prospective controlled clinical trial
- Author
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Unal, Yilmaz, primary and Kilinc, Muhammet Fatih, additional
- Published
- 2018
- Full Text
- View/download PDF
14. Rectal application of argan oil improves healing of colorectal anastomosis in rats
- Author
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Barlas, Aziz Mutlu, primary, Kuru, Serdar, additional, Kismet, Kemal, additional, Cavusoglu, Turgut, additional, Bag, Yusuf Murat, additional, Senes, Mehmet, additional, Cihan, Neslihan, additional, Celepli, Pinar, additional, Unal, Yilmaz, additional, and Hucumenoglu, Sema, additional
- Published
- 2018
- Full Text
- View/download PDF
15. The Effect of Calcium Dobesilate on Liver Damage in Experimental Obstructive Jaundice
- Author
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Unal, Yilmaz, primary, Tuncal, Salih, additional, Kosmaz, Koray, additional, Kucuk, Berkay, additional, Kismet, Kemal, additional, CAVUSOGLU, Turgut, additional, Celepli, Pinar, additional, Senes, Mehmet, additional, Yildiz, Selin, additional, and Hucumenoglu, Sema, additional
- Published
- 2018
- Full Text
- View/download PDF
16. Does inguinal hernia repair affect uroflowmetric parameters? A prospective controlled clinical trial.
- Author
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Unal, Yilmaz and Kilinc, Muhammet Fatih
- Subjects
- *
INGUINAL hernia , *CLINICAL trials , *URINATION disorders , *RETENTION of urine - Abstract
Objective: The aim of this study was to determine the changes in uroflowmetric values of male patients following elective inguinal hernia repair. Methods: The study group comprised patients treated with open or laparoscopic inguinal hernia repair. Patients who underwent surgery due to various diseases such as cataract, multinodular goiter, choledocholithiasis, thyroid and breast cancer, hydrocele, or spermatocele were recruited to the control group. Perioperative characteristics, including pain assessed using the visual analog scale (VAS), uroflowmetric values, and post‐void residual urine volumes (PVR), were recorded preoperatively and on Postoperative Days (POD) 1 and 30. Uroflowmetry, VAS, and PVR values were also recorded for the control group on Study Days 1, 3, and 30. Results: There were 103 subjects in this study (52 treated with elective inguinal hernia repair in the study group, 51 in the control group). Preoperative VAS, maximum and average flow rate, and PVR were similar in the 2 groups (P > .05 for all), whereas comparisons of these 3 factors on POD 1 revealed significant differences (P < .001 for all). Linear regression analysis demonstrated that preoperative maximum and POD 1 VAS values of patients who underwent elective inguinal hernia repair could significantly predict acute urinary retention or voiding dysfunction (P = .001 for all). Conclusion: Postoperative pain affect parameters and cause urinary retention after open or laparoscopic inguinal hernia repair. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
17. The Effect of Calcium Dobesilate on Liver Damage in Experimental Obstructive Jaundice.
- Author
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Unal, Yilmaz, Tuncal, Salih, Kosmaz, Koray, Kucuk, Berkay, Kismet, Kemal, CAVUSOGLU, Turgut, Celepli, Pinar, Senes, Mehmet, Yildiz, Selin, and Hucumenoglu, Sema
- Subjects
- *
OBSTRUCTIVE jaundice , *BILIARY tract , *LIVER , *CALCIUM , *BILE ducts - Abstract
Purpose/Aim of the study: Inflammation and oxidative stress are two significant factors affecting the degree of liver damage in obstructive jaundice. The aim of this study was to evaluate the effect of calcium dobesilate (CaDob), an effective antioxidant and anti-inflammatory drug, on damage to liver caused by experimental obstructive jaundice. Materials and Methods: 30 rats in total were randomly placed into three groups, each group consisting of 10 rats. The sham group (Group 1) only received solely laparotomy. In the control group (Group 2), ligation was applied to the biliary tract and no treatment was implemented. In the CaDob group (Group 3), following ligation of the biliary tract, 100 mg/kg/day CaDob was implemented via an orogastric tube for a 10–day period. Liver tissue and blood samples were taken for histopathological and biochemical examination. Results: The CaDob group had significantly lower test values for serum liver functions when compared to the control group. Statistically lower levels of tissue malondialdehyde (MDA) and fluorescent oxidation products (FOP) were detected in the CaDob group, and the CaDob group had significantly higher levels of sulfydryl (SH) than the control group. Histopathological scores in the CaDob group were found out to be statistically less than the scores the control group received (p < 0.05). Conclusions: CaDob treatment repaired the histpatological changes induced by bile duct ligation. The hepatoprotective effects of CaDob can be associated with its antioxidant properties of the drug. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
18. Investigation of the Presence of Francisella tularensis by Culture, Serology and Molecular Methods in Mice of Thrace Region, Turkey
- Author
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Şaban Gürcan, Gülizar Unal Yilmaz, Aynur Karadenizli, and Beytullah Ozkan
- Subjects
Microbiology (medical) ,Mus macedonicus ,Male ,Turkey ,Spleen ,Biology ,Microbiology ,Serology ,law.invention ,Tularemia ,Rodent Diseases ,Mice ,law ,Risk Factors ,Zoonoses ,medicine ,Animals ,Humans ,Water tanks ,Francisella tularensis ,Polymerase chain reaction ,Disease Reservoirs ,General Immunology and Microbiology ,biology.organism_classification ,medicine.disease ,Virology ,Infectious Diseases ,medicine.anatomical_structure ,Liver ,Apodemus ,Female ,Murinae - Abstract
Tularemia is a disease that has been reported in Turkey since 1936. Although mice are considered to have a role in the transmission of Francisella tularensis to man, this has not been exactly confirmed yet. The aim of this study was to investigate the presence of F. tularensis in mice by using culture, serology and molecular methods. For this purpose, four villages (Edirne-Demirkoy, Kirklareli-Kaynarca, Tekirdag-Muzruplu, Tekirdag-Sinanli) were selected in Thrace Region of Turkey where tularemia cases had been reported previously. A total of 126 live-catch mouse traps were established in warehouses, barns, areas near wells, water tanks and creeks in the villages in December 2012. Traps were kept overnight and the next day the animals collected were identified at species-level. The live-captured mice were anesthetized and their heart blood samples were obtained. Subsequently, liver and spleen tissues were removed from every mouse under aseptic conditions in the class-2 safety cabinet. These tissues were cultivated in Francis medium containing 5% sheep blood, 0.1% cystein, 1% glucose and incubated for seven days in both normal atmosphere and 5% carbondioxide incubator at 37°C. Tularemia microagglutination test was performed by using the sera which were obtained from live-captured mice. Finally, DNAs were isolated from both liver and spleen tissues of mice, and real-time polymerase chain reaction (Tularemia RT-PCR; Public Health Agency of Turkey, Ankara) were performed. In our study, a total of 19 mice were captured and of these 11 were alive. Ten mice were identified as Apodemus flavicollis, seven were Mus macedonicus and two were Mus musculus. There were no Francisella tularensis isolation in the cultures of mice liver and spleen tissues. Serological tests yielded negative results for 10 mice whose serum samples could be obtained. In RT-PCR, positivity were detected in spleen tissues of two mice which were captured from Kaynarca where first tularemia cases in Turkey in 1936 were reported but has no report from then on. One of them was a live female Mus macedonicus, and the other was a dead male Apodemus flavicollis. In quantitative evaluation, number of microorganism per organ were calculated as 4 x 103 cfu/spleen in Mus macedonicus and 4 x 104 cfu/spleen in Apodemus flavicollis. This is the first study in Turkey indicating that the mice in natural environment harbored F.tularensis. In conclusion, the results of this study indicated that the agent of tularemia has been retained since 1936 in Kaynarca region and this persistence might present a potential risk for tularemia epidemics.
19. [Investigation of the presence of Francisella tularensis by culture, serology and molecular methods in mice of Thrace Region, Turkey].
- Author
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Unal Yilmaz G, Gurcan S, Ozkan B, and Karadenizli A
- Subjects
- Animals, Disease Reservoirs, Female, Francisella tularensis genetics, Francisella tularensis immunology, Humans, Liver microbiology, Male, Mice, Risk Factors, Rodent Diseases microbiology, Rodent Diseases transmission, Spleen microbiology, Tularemia transmission, Turkey epidemiology, Francisella tularensis isolation & purification, Murinae, Rodent Diseases epidemiology, Tularemia epidemiology, Zoonoses epidemiology, Zoonoses microbiology, Zoonoses transmission
- Abstract
Tularemia is a disease that has been reported in Turkey since 1936. Although mice are considered to have a role in the transmission of Francisella tularensis to man, this has not been exactly confirmed yet. The aim of this study was to investigate the presence of F. tularensis in mice by using culture, serology and molecular methods. For this purpose, four villages (Edirne-Demirkoy, Kirklareli-Kaynarca, Tekirdag-Muzruplu, Tekirdag-Sinanli) were selected in Thrace Region of Turkey where tularemia cases had been reported previously. A total of 126 live-catch mouse traps were established in warehouses, barns, areas near wells, water tanks and creeks in the villages in December 2012. Traps were kept overnight and the next day the animals collected were identified at species-level. The live-captured mice were anesthetized and their heart blood samples were obtained. Subsequently, liver and spleen tissues were removed from every mouse under aseptic conditions in the class-2 safety cabinet. These tissues were cultivated in Francis medium containing 5% sheep blood, 0.1% cystein, 1% glucose and incubated for seven days in both normal atmosphere and 5% carbondioxide incubator at 37°C. Tularemia microagglutination test was performed by using the sera which were obtained from live-captured mice. Finally, DNAs were isolated from both liver and spleen tissues of mice, and real-time polymerase chain reaction (Tularemia RT-PCR; Public Health Agency of Turkey, Ankara) were performed. In our study, a total of 19 mice were captured and of these 11 were alive. Ten mice were identified as Apodemus flavicollis, seven were Mus macedonicus and two were Mus musculus. There were no Francisella tularensis isolation in the cultures of mice liver and spleen tissues. Serological tests yielded negative results for 10 mice whose serum samples could be obtained. In RT-PCR, positivity were detected in spleen tissues of two mice which were captured from Kaynarca where first tularemia cases in Turkey in 1936 were reported but has no report from then on. One of them was a live female Mus macedonicus, and the other was a dead male Apodemus flavicollis. In quantitative evaluation, number of microorganism per organ were calculated as 4 x 103 cfu/spleen in Mus macedonicus and 4 x 104 cfu/spleen in Apodemus flavicollis. This is the first study in Turkey indicating that the mice in natural environment harbored F.tularensis. In conclusion, the results of this study indicated that the agent of tularemia has been retained since 1936 in Kaynarca region and this persistence might present a potential risk for tularemia epidemics.
- Published
- 2014
- Full Text
- View/download PDF
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