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29 results on '"Umlauf Scott"'

1. Atypical Asparagine Deamidation of NW Motif Significantly Attenuates the Biological Activities of an Antibody Drug Conjugate

Author

Cao, Mingyan, primary, Hussmann, G. Patrick, additional, Tao, Yeqing, additional, O’Connor, Ellen, additional, Parthemore, Conner, additional, Zhang-Hulsey, Diana, additional, Liu, Dengfeng, additional, Jiao, Yang, additional, Mel, Niluka de, additional, Prophet, Meagan, additional, Korman, Samuel, additional, Sonawane, Jaytee, additional, Grigoriadou, Christina, additional, Huang, Yue, additional, Umlauf, Scott, additional, and Chen, Xiaoyu, additional

Published
2023
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5. ChAdOx1 interacts with CAR and PF4 with implications for thrombosis with thrombocytopenia syndrome

Author

Baker, Alexander T., primary, Boyd, Ryan J., additional, Sarkar, Daipayan, additional, Teijeira-Crespo, Alicia, additional, Chan, Chun Kit, additional, Bates, Emily, additional, Waraich, Kasim, additional, Vant, John, additional, Wilson, Eric, additional, Truong, Chloe D., additional, Lipka-Lloyd, Magdalena, additional, Fromme, Petra, additional, Vermaas, Josh, additional, Williams, Dewight, additional, Machiesky, LeeAnn, additional, Heurich, Meike, additional, Nagalo, Bolni M., additional, Coughlan, Lynda, additional, Umlauf, Scott, additional, Chiu, Po-Lin, additional, Rizkallah, Pierre J., additional, Cohen, Taylor S., additional, Parker, Alan L., additional, Singharoy, Abhishek, additional, and Borad, Mitesh J., additional

Published
2021
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6. Superior efficacy of a recombinant flagellin:H5N1 HA globular head vaccine is determined by the placement of the globular head within flagellin

Author

Song, Langzhou, Zhang, Yi, Yun, Nadezhda E., Poussard, Allison L., Smith, Jeanon N., Smith, Jennifer K., Borisevich, Viktoriya, Linde, Jenna J., Zacks, Michele A., Li, Hong, Kavita, Uma, Reiserova, Lucia, Liu, Xiangyu, Dumuren, Kunmi, Balasubramanian, Bhuvaneswari, Weaver, Bruce, Parent, Jason, Umlauf, Scott, Liu, Ge, Huleatt, Jim, Tussey, Lynda, and Paessler, Slobodan

Published
2009
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9. Site-Specific Recombination Promoted in Vitro by the FLP Protein of the Yeast Two-Micron Plasmid

Author

Senecoff, Julie F., Bruckner, Robert C., Meyer-Leon, Leslie, Gates, Cynthia A., Wood, Elizabeth, Umlauf, Scott W., Attwood, Janet M., Cox, Michael M., Hollaender, Alexander, editor, Wickner, Reed B., editor, Hinnebusch, Alan, editor, Lambowitz, Alan M., editor, Gunsalus, I. C., editor, Preer, John R., Jr., editor, Mets, Laurens, editor, Gumport, Richard I., editor, Wilson, Claire M., editor, and Kuny, Gregory, editor

Published
1986
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10. Potency Assay Variability Estimation in Practice.

Author

Li, Hang, Witkos, Tomasz M., Umlauf, Scott, and Thompson, Christopher

Subjects
*BIOLOGICAL assay, *DRUG development
Abstract

During the drug development process, testing potency plays an important role in the quality assessment required for the manufacturing and marketing of biologics. Due to multiple operational and biological factors, higher variability is usually observed in bioassays compared with physicochemical methods. In this paper, we discuss different sources of bioassay variability and how this variability can be statistically estimated. In addition, we propose an algorithm to estimate the variability of reportable results associated with different numbers of runs and their corresponding OOS rates under a given specification. Numerical experiments are conducted on multiple assay formats to elucidate the empirical distribution of bioassay variability. [ABSTRACT FROM AUTHOR]

Published
2024
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16. Efficacious Recombinant Influenza Vaccines Produced by High Yield Bacterial Expression: A Solution to Global Pandemic and Seasonal Needs

Author

Song, Langzhou, primary, Nakaar, Valerian, additional, Kavita, Uma, additional, Price, Albert, additional, Huleatt, Jim, additional, Tang, Jie, additional, Jacobs, Andrea, additional, Liu, Ge, additional, Huang, Yan, additional, Desai, Priyanka, additional, Maksymiuk, Gail, additional, Takahashi, Virginia, additional, Umlauf, Scott, additional, Reiserova, Lucia, additional, Bell, Rodney, additional, Li, Hong, additional, Zhang, Yi, additional, McDonald, William F., additional, Powell, T. J., additional, and Tussey, Lynda, additional

Published
2008
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21. Efficacious Recombinant Influenza Vaccines Produced by High Yield Bacterial Expression: A Solution to Global Pandemic and Seasonal Needs.

Author

Langzhou Song, Nakaar, Valerian, Kavita, Uma, Price, Albert, Huleatt, Jim, Jie Tang, Jacobs, Andrea, Ge Liu, Yan Huang, Desai, Priyanka, Maksymiuk, Gail, Takahashi, Virginia, Umlauf, Scott, Reiserova, Lucia, Bell, Rodney, Hong Li, Yi Zhang, McDonald, William F., Powell, T. J., and Tussey, Lynda

Subjects
INFLUENZA vaccines, LIGANDS (Biochemistry), ANTIGENS, IMMUNOLOGICAL adjuvants, HEMAGGLUTININ, HUMAN genetic variation, ESCHERICHIA coli, FERMENTATION, PANDEMICS
Abstract

It is known that physical linkage of TLR ligands and vaccine antigens significantly enhances the immunopotency of the linked antigens. We have used this approach to generate novel influenza vaccines that fuse the globular head domain of the protective hemagglutinin (HA) antigen with the potent TLR5 ligand, flagellin. These fusion proteins are efficiently expressed in standard E. coli fermentation systems and the HA moiety can be faithfully refolded to take on the native conformation of the globular head. In mouse models of influenza infection, the vaccines elicit robust antibody responses that mitigate disease and protect mice from lethal challenge. These immunologically potent vaccines can be efficiently manufactured to support pandemic response, pre-pandemic and seasonal vaccines. [ABSTRACT FROM AUTHOR]

Published
2008
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22. Plasmid Vectors Encoding Cholera Toxin or the Heat-Labile Enterotoxin from Escherichia coliAre Strong Adjuvants for DNA Vaccines

Author

Arrington, Joshua, Braun, Ralph P., Dong, Lichun, Fuller, Deborah H., Macklin, Michael D., Umlauf, Scott W., Wagner, Sarah J., Wu, Mary S., Payne, Lendon G., and Haynes, Joel R.

Abstract

ABSTRACTTwo plasmid vectors encoding the A and B subunits of cholera toxin (CT) and two additional vectors encoding the A and B subunits of the Escherichia coliheat-labile enterotoxin (LT) were evaluated for their ability to serve as genetic adjuvants for particle-mediated DNA vaccines administered to the epidermis of laboratory animals. Both the CT and the LT vectors strongly augmented Th1 cytokine responses (gamma interferon [IFN-?]) to multiple viral antigens when codelivered with DNA vaccines. In addition, Th2 cytokine responses (interleukin 4 [IL-4]) were also augmented by both sets of vectors, with the effects of the LT vectors on IL-4 responses being more antigen dependent. The activities of both sets of vectors on antibody responses were antigen dependent and ranged from no effect to sharp reductions in the immunoglobulin G1 (IgG1)-to-IgG2a ratios. Overall, the LT vectors exhibited stronger adjuvant effects in terms of T-cell responses than did the CT vectors, and this was correlated with the induction of greater levels of cyclic AMP by the LT vectors following vector transfection into cultured cells. The adjuvant effects observed in vivo were due to the biological effects of the encoded proteins and not due to CpG motifs in the bacterial genes. Interestingly, the individual LT A and B subunit vectors exhibited partial adjuvant activity that was strongly influenced by the presence or absence of signal peptide coding sequences directing the encoded subunit to either intracellular or extracellular locations. Particle-mediated delivery of either the CT or LT adjuvant vectors in rodents and domestic pigs was well tolerated, suggesting that bacterial toxin-based genetic adjuvants may be a safe and effective strategy to enhance the potency of both prophylactic and therapeutic DNA vaccines for the induction of strong cellular immunity.

Published
2002
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29. Plasmid vectors encoding cholera toxin or the heat-labile enterotoxin from Escherichia coli are strong adjuvants for DNA vaccines.

Author

Arrington J, Braun RP, Dong L, Fuller DH, Macklin MD, Umlauf SW, Wagner SJ, Wu MS, Payne LG, and Haynes JR

Subjects
AIDS Vaccines genetics, AIDS Vaccines immunology, Animals, Antibodies, Viral blood, Bacterial Toxins administration & dosage, Caco-2 Cells, Cholera Toxin administration & dosage, Enterotoxins administration & dosage, HIV Envelope Protein gp120 genetics, HIV Envelope Protein gp120 immunology, Humans, Immunization, Influenza Vaccines genetics, Influenza Vaccines immunology, Mice, Plasmids genetics, Swine, T-Lymphocytes immunology, Transfection, Vaccines, DNA administration & dosage, Viral Matrix Proteins genetics, Viral Matrix Proteins immunology, Adjuvants, Immunologic, Bacterial Toxins genetics, Cholera Toxin genetics, Enterotoxins genetics, Escherichia coli Proteins, Genetic Vectors administration & dosage, Vaccines, DNA immunology
Abstract

Two plasmid vectors encoding the A and B subunits of cholera toxin (CT) and two additional vectors encoding the A and B subunits of the Escherichia coli heat-labile enterotoxin (LT) were evaluated for their ability to serve as genetic adjuvants for particle-mediated DNA vaccines administered to the epidermis of laboratory animals. Both the CT and the LT vectors strongly augmented Th1 cytokine responses (gamma interferon [IFN-gamma]) to multiple viral antigens when codelivered with DNA vaccines. In addition, Th2 cytokine responses (interleukin 4 [IL-4]) were also augmented by both sets of vectors, with the effects of the LT vectors on IL-4 responses being more antigen dependent. The activities of both sets of vectors on antibody responses were antigen dependent and ranged from no effect to sharp reductions in the immunoglobulin G1 (IgG1)-to-IgG2a ratios. Overall, the LT vectors exhibited stronger adjuvant effects in terms of T-cell responses than did the CT vectors, and this was correlated with the induction of greater levels of cyclic AMP by the LT vectors following vector transfection into cultured cells. The adjuvant effects observed in vivo were due to the biological effects of the encoded proteins and not due to CpG motifs in the bacterial genes. Interestingly, the individual LT A and B subunit vectors exhibited partial adjuvant activity that was strongly influenced by the presence or absence of signal peptide coding sequences directing the encoded subunit to either intracellular or extracellular locations. Particle-mediated delivery of either the CT or LT adjuvant vectors in rodents and domestic pigs was well tolerated, suggesting that bacterial toxin-based genetic adjuvants may be a safe and effective strategy to enhance the potency of both prophylactic and therapeutic DNA vaccines for the induction of strong cellular immunity.

Published
2002
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