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Objective: To estimate the incidence of dry eye in post-operative patient undergoing cataract surgery. Materials and Methods: Schirmer’s test, tear break-up time (TBUT) test, and lissamine green staining of the cornea and conjunctiva for the evaluation of dry eye preoperatively and again at first and second follow-up examinations at 1 week and 1 month after cataract surgery were performed on 100 patients with senile cataract. Results: The patients were 61.25 years old on average, and 71 (71%) of them were men. According to the requirements of our study, none of the patients experienced dry eyes at the time of participation. Schirmer’s test results during the first and second follow-ups, respectively, varied from 12-35 mm and 8-24 mm postoperatively. At the first and second follow-ups, the mean TBUT was 13.12 ± 2.44 and 9.61 ± 2.22 seconds, respectively, whereas the lissamine green staining score was 3 in 66 (66%) and 1 in 62 (62%) patients, respectively. At the initial follow-up, grade 2 dry eye was discovered in 89% of the 56 patients who received phacoemulsification (p 0.001) and in 92% of the 66 patients who underwent small-incision cataract surgery (SICS) (p 0.001). At the second follow-up, 83% of patients who had SICS and 93% of those who had phacoemulsification had grade 0 dry eyes, respectively (p 0.001). Conclusion: The incidence of dry eye following cataract surgery was significant and largely unrelated to demographic and anthropometric characteristics, surgical technique, length of microscope exposure, and energy input. This dryness was only temporary in character and exhibited a downward trend, with a tendency to return to normal by the end of 1 month.

Abstract The purpose of this study is to analyze the impact of dimension of dynamic capabilities such as Entrepreneurial orientation (EO), technological innovation (TIN), and digital transformation (DT) and dimension of Stakeholder environment, society, and governance (ESG) practices on environmental performance (EP) in the handicraft industry. This study also investigates the mediating impact of dynamic capabilities such as Technological innovation (TIN) and Entrepreneurial orientation between ESG and EP and has applied the Sobel test. This study also checks the moderating impact of Digital transformation (DT) on EP through TIN. The Sobel test is most suitable for mediation analysis, whether partial or complete mediation. For this study, exploratory research methods , convenience sampling, and tools used a 5-point Likert scale. Therefore, the study was well suited to this type of experimental research, since it used the PLS–SEM path coefficient algorithm and PCA analysis software to examine several correlations simultaneously. A total of 150 respondents participated in this survey who were artisan’s new entrepreneurs and firm owners in the handicraft industry. Because of their outsized influence on emissions worldwide and consumption of resources, businesses worldwide have a responsibility to ensure environmental stability while also working to improve their practices to improve the craft industry as part of the small industry. These findings prove that by adopting ESG principles and Entrepreneurial orientation, the impact of technological innovation supports the environment's performance. The development of eco-friendly goods and procedures, the enhancement of craft productivity, and the evolution of sustainable development strategies are all greatly aided by digital transformation, technological innovation handicraft business environmental sustainability in the face of the escalating difficulties brought on by climate change. For verification and exploration of entrepreneurial orientation, technological innovation, Environmental performance, and environmental social and governance (ESG), this study is supported by the Dynamic capability theory (DCT), stakeholder theory, and especially ESG criteria. The research shows that firms' environmental performance can be significantly improved by emphasizing ESG factors and embracing technological advancements and entrepreneurial orientation.

Abstract Numerous neurological disorders are linked to sequences rich in guanine repeats found in introns, exons, and regulatory regions of genes. These sequences have been observed to form stable G-quadruplex (GQ) structures both in vitro and in vitro. Cerebellar Ataxia with Neuropathy and Vestibular Areflexia Syndrome (CANVAS), a slowly progressive neurodegenerative disorder, is associated with the biallelic expansion of (AAGGG)n pathogenic repeats in the second intron of the RFC1 gene. Though these G-rich pathogenic repeats in other neurological diseases are associated with protein loss of function, RNA gain of function, and/or protein gain of function, not much is known about the pathological mechanism associated with CANVAS. Herein, we report the formation of stable GQ conformations in the CANVAS-associated repeats i.e., r(AAGGG)n, where ‘r’ stands for RNA. These GQs are critical regulators in neurological disorders leading to RNA foci formation and RNA binding protein sequestration. They also alter other causative processes like intron retention, which leads us to hypothesize a toxic Proteinopathy mechanism in CANVAS. Various biophysical and biomolecular assays characterized the interactions of three aggregation-prone RNA-binding proteins (RBPs): heterogeneous nuclear ribonucleoprotein H1/F (hnRNP H1/F), and DGCR8 with different pathogenic repeats [(AAGGG)9] in vitro, further affirming the hypothesis. The biophysical observations are further supported by molecular dynamics analysis and cell-based studies, putting us a step closer to elucidating the pathological mechanism(s) in CANVAS neuropathy, paving the way for the development of innovative therapeutic interventions.

Abstract The purpose of this paper is to analyze the impact of various dynamic capabilities (Innovation (IN), entrepreneurial leadership (EL), Social media (SM), and digital technology (DT)) on Business performance (BP). This study also analysis the mediating impact of talent management(TM), and Entrepreneurial orientation (EO) between EL, and Business performance. The reason for this is that in the handicraft industry SM, DT TM, and SR impact on business performance are still not fully understood. We have adopted a survey-based methodology from 410 handicraft artisans or entrepreneurs. Data was empirically analyzed to test the theoretical model of mediation through Smart partial least square method (SMART PLS-SEM) software. The study's findings prove entrepreneurial EL improves Business performance whereas the association between Social media (SM) and BP is insignificant. The mediating impact of Talent management, EO, and strategic resilience was statistically positive and significant and proved that bridging between BP and all three mediators supports and makes stronger EL for boosting business performance. Innovation, and digital technology, positively affect Business performance. This study explores artisans' formal entrepreneurial EL style. This study is unique because the Dynamic capability theory (DCT) as an extension of the resource-based view (RBV) has been used for critical business factors like EL, strategic resilience, Business performance, digital technology, innovation, and talent management in the handicraft sector will be extended. This study also proposes a new Digital capability view (DCV) for future study. This study will explore the theoretical and practical understating of (DCT) with managerial use of EL, Innovation, and TM for boosting Business performance.

Multidimensional manganese-metal organic frameworks (Mn-MOF) are synthesized using 1,2,4,5-Benzene tetracarboxylic acid (BTTC) at various temperatures (100–160 °C). The Fourier-transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD) techniques successfully confirm the formation of Mn-MOF. Among the various temperatures, the Mn-MOF synthesized at 140 °C (Mn-MOF@BTTC-140) is remarkable because it has excellent crystallinity and a unique morphology, i.e., woodchips-like structure. The synthesized Mn-MOF@BTTC materials are used in supercapacitor applications. In comparison to all materials, Mn-MOF@BTTC-140 revealed the maximum specific capacitance (Cs) of 627 F g-1 @ 1 A g-1, and it displayed 91 % capacitance retention even after the 6000 cycles at a current density of 10 A g-1. Furthermore, the supercapacitor device (SD) constructed using carbon nanofibers (CNF) as the negative electrode and Mn-MOF@BTTC-140 as the positive electrode delivered an energy density of 25 W h kg-1 at a power density of 532 W kg-1. Ultimately, LED lighting demonstrates that our fabricated materials suit practical applications.

Background: The crucial aspect to consider during method development and validation, ensuring accurate, precise, and specific estimation of drug substances and drug products, is stability. Various factors, including environmental, instrumental, reagent, and human factors, can pose challenges in achieving suitable method development and validation. Objective: This work aimed to develop and validate a low flow rate, LCMS compatible, simple, and rapid reverse-phase high-performance liquid chromatographic method for estimating efavirenz and its degradation products at different stress conditions. Materials and Methods: The HPLC system employed a Phenomenex Luna 5μ C18 (2) 100A (250 x 4.6 mm) column and a mobile phase of methanol: 20 millimolar ammonium formate solution (90:10) adjusted to pH 4 with formic acid. All analytes were separated within 15 minutes and detected at 247 nm. Method validation was carried out according to ICH guidelines, including linearity, accuracy, precision, ruggedness, robustness, LOD, and LOQ. Results and Discussion: The method was linear in the 10-90 μg/ml range, with a regression coefficient 0.999. Intra- and inter-day precisions, ruggedness, and robustness were within acceptable limits (≤2% RSD) with LOD and LOQ of 0.35 and 1.16 μg/ml, respectively. Degradation study indicates well resolution of the drug and degradants. Conclusion: Purposeful degradation of efavirenz resulted in different degradation products under various stress conditions, and the method demonstrated satisfactory resolution from its degradants.

A wide working potential range of asymmetric capacitor devices with solid state gel electrolytes have enormous advantages of energy storage devices like high capacity, long life and high-power density. Here, the sandwiched type rGO@MnCo2S4//rGO asymmetric supercapacitor (ASC) electrode was constructed and functioned with a wide potential of 1.6 V. The electrochemical evaluation was verified through a three-electrode operating system, it shows the MnCo2S4 and rGO@MnCo2S4 electrodes have 42.72 mAh g-1 and 82.57 mAh g-1 capacity values. Moreover, the constructed rGO@MnCo2S4//rGO ASC device shows a higher capacity of 140.83 mAh g-1 at 1 A g-1. In addition, the rGO@MnCo2S4//rGO ASC device also delivers a higher energy density of 37.55 Wh kg-1 and power density of 800 W kg-1. However, the stability of the rGO@MnCo2S4//rGO ASC device shows about 93 % cyclic retention after 3000 cycles. These results may show the rGO@MnCo2S4 electrode is an opted electrode material for supercapacitor applications.

Tomato leaf miner, Tuta absoluta (Meyrick, 1917), is an invasive insect pest that was introduced to India from South America wherein its initial occurrence in India was recorded in tomato fields of Pune, Maharashtra in October 2014. However, it was first observed in Northern India in 2016, with no prior reports from Jammu region until 2022. This article documents the first recorded instance of T. absoluta infestation on tomatoes in Jammu region. The survey was conducted for two consecutive years (2022 and 2023) during the tomato cropping period (March-June), encompassing four different districts of Jammu regions viz., Udhampur, Doda, Ramban and Jammu. This research provides valuable insights into spread and impact of T. absoluta in the Jammu region. The consolidated picture of two-year data revealed highest leaf and fruit infestation in district Udhampur, followed by Doda and Jammu districts, respectively. Curiously, there were no records of T. absoluta infestations in Ramban district. To gain a better understanding of biology and morphometrics of this pest, larvae were collected and reared in the laboratory of Division of Entomology, SKUAST-Jammu. The crucial data on biological and morphometric attributes were collected which can facilitate further investigations and development of potential management strategies.

Societal Impact Statement Preserving and conserving crop landraces, tended by indigenous farming communities, is crucial for future food security. This research focused on rice landrace diversity in the north‐eastern Himalayan region of Nagaland, India, where Naga communities cultivate rice according to their dietary and cultural preferences. Rice diversity is closely linked to the region's ethnolinguistic and ecological variety. On‐farm conservation, in collaboration with indigenous communities, is imperative to protect these germplasm resources. Involving these communities actively in conservation efforts will safeguard their traditional knowledge, endorse sustainable farming practices, and enhance the resilience of local agricultural systems. Summary Understanding the genetic diversity and cultural significance of crop landraces is crucial for their conservation and sustainable utilization. This study focused on rice landraces from Nagaland in north‐eastern India to assess their genetic diversity and explore their associations with ethnolinguistic groups. We collected 78 rice landraces from Nagaland and a small part of Manipur and conducted microsatellite genotyping for genetic analysis. We integrated social anthropology and population genetics analyses of rice landraces to glean insights into the genetic diversity, population structure, and ethnolinguistic history of rice cultivation in Nagaland. The study revealed the rich cultural significance of rice landraces among the Nagas. Farmers practiced small‐scale subsistence farming, maintaining diverse rice landraces. Naming conventions were based on factors such as seed source, color, grain type, and ecological suitability. Rice landraces played important roles in ethnic cultures, festivals, and religious ceremonies. Genetic analysis identified significant diversity, with 277 alleles across 69 loci and a moderate gene diversity of 0.57. Two distinct sub‐populations were identified, with one dominated by Chakhesang and Angami Nagas and the other by Sümi and Lotha accessions. Differentiation was observed between lowland and upland cultivars, with one sub‐population comprising exclusively lowland varieties. Cultural factors and cultivation practices influenced population differentiation, with ethnicity and ecotype having a significant impact. The study also highlighted the correlation between ethnolinguistic differentiation and the indica–japonica structuring of rice landraces. Different ethnic groups in Nagaland had distinct cultivation practices, contributing to genetic differentiation. Overall, this research emphasizes the need to preserve rice landraces and associated traditional knowledge for future improvements and cultural heritage conservation. It provides insights into genetic diversity, cultural significance, and the relationship between genetic diversity, cultural practices, and agricultural traditions.

Atmospheric CO2 removal (CDR) is a fundamentally endergonic process. Performing CDR or Bioenergy with Carbon Capture and Storage (BECCS) at the gigatonne scale will produce a significant additional burden on the planet’s limited renewable energy resources irrespective of the technology employed. Harnessing photosynthesis to drive industrial-scale CO2 fixation has been of significant interest because of its minimal energy requirements and potential low costs. In this review, we evaluated the thermodynamic considerations of performing atmospheric carbon removal using microalgae and cyanobacteria versus physicochemical processes and explore the implications of these energetic costs on the scalability of each respective solution. We review the biomass productivities of recently discovered fast-growing cyanobacterial strains and discuss the prospects of genetically engineering certain metabolic pathways for channeling the fixed carbon into metabolic ‘carbon sinks’ to further enhance their CO2 capture while concurrently extracting value. We share our perspectives on how new highly productive chassis strains combined with advanced flux balance models, essentially coupling synthetic biology with industrial biotechnology, may unlock more favorable methods for CDR, both from an economic and thermodynamic perspective.

The less emission, environmental friendliness and less hazardous energy storage materials are most impotent to the clean energy system. Here we synthesis rGO anchored nickel vanadate nanorods for supercapacitor application. As a result, the Ni2V2O7 and rGO@Ni2V2O7 nanomaterials show a monoclinic structure from the XRD pattern. The vibrational of V–O–V and Ni–O along with the main peak of carbon materials indicates the rGO anchoring with Ni2V2O7 in the FTIR and Raman spectra. The SEM and TEM images illustrate a rod shaped Ni2V2O7 was attached over the rGO sheet surface. From the BET analysis, the rGO@Ni2V2O7 sample has a higher surface area than the Ni2V2O7 electrode. Further, the supercapacitor application of Ni2V2O7 and rGO@Ni2V2O7 electrodes shows 318 F/g and 624 F/g at 1 A/g capacitance values. Although, the rGO@Ni2V2O7 electrode shows higher power (771 W/kg) and energy density (22.28 Wh/kg) values than the NiV2O7 electrode. Moreover, the rGO@Ni2V2O7 exhibits a 95% of capacitance retention value even at the 1000th cycle. Moreover, the prepared rGO@Ni2V2O7 has less Rct value of 4.72 Ω) than the Ni2V2O7 electrode (9.86 Ω). The attachment of rGO can provide a higher surface area, higher conductivity and lower resistivity to the Ni2V2O7 electrode. According to these results, the rGO@Ni2V2O7 electrode is the more suitable for supercapacitor electrodes.

Abstract This research focuses on the factors that barriers and foster (drivers) digital innovation and entrepreneurship amongst small businesses operating in the Handicraft industry after the economic downturn. From the perspective of new Indian craft entrepreneurs, digital and innovative entrepreneurship is crucial to surviving the crisis. It will eventually encourage the development of the handmade goods market and the artisanal reconfiguration as a business. In this study, the qualitative method was used. For the creation of themes, Inductive content analysis (analytical, inductive technique) was used to interview fifty online handicraft business owners who rely on their craft talents for income. After the pandemic, two types of motivation were generated for handmade entrepreneurs. The first motivation was extrinsic, which conditioned the handicraft market, restricted mobility, and upgraded household status for entrepreneurship. The second was intrinsic motivation among the craft entrepreneurs who generated individual and professional skill growth. These two factors encouraged digital craft innovations and entrepreneurial advancement of transitioning handicraft companies to digital platforms. After the interview, respondents explained several hindrances or restrictions, market interruption, and quality internet. Even after that, it resolved as the effect of the pandemic reduced and digital skill training for innovation and entrepreneurship passion increased for developing a robust programme that supports the handicraft new entrepreneurs after the economic calamities. This finding contributes to the area of investigation and contributes to entrepreneurship literature. This study will open the route for making the policy and development of solid strategic plans for the handicraft sector’s new craft entrepreneurs. With the help of innovative and digital entrepreneurs, small-scale or craft industries will boost the Indian economy and give some of the best suggestions for global economies. For developing economies, this study will also empower entrepreneurs to open new dimensions for marketing, supply chain, and exportation and will finally increase the Globalisation of handmade products.

Ficus L. is a genus in Moraceae with 115 taxa from all over India and plays a key role in Indian ethnobotany. The Khasi and Jaintia hill in Meghalaya is rich in flora including the genus Ficus L. and have diverse cultures among local tribes. The uses, coverage, and cultural importance of Ficus can be expressed through several quantitative ethnobiological indices such as TIV, RFC, CI, and UV. This paper highlights the indices for ten species of Ficus used by the tribal people in the Khasi and Jaintia hills of Meghalaya which shows that the genus has higher ethnobotanical value.

Abstract In the current scenario, the level of interest is increasing in female entrepreneurship in the handicraft sector worldwide. So the primary purpose of this study is to discover the different constructs that influence women entrepreneurs in India as learner students or after completion of their degree, either skill degree or professional degree. Female entrepreneurship is an important feature of economic progress, particularly in developing nations, since it provides opportunities for innovation, cultural and traditional connectivity, job creation, poverty reduction, socio-economic growth, etc. However, despite its significance, this study has garnered little attention in the entrepreneurship literature. For this research, we used Survey research and convenience sampling. For data analysis, AMOS 25 software was used; where needed, we used an Excel sheet for table formation. At the same time, traditional cultural constraints have tended to study Indian women’s participation in handicraft entrepreneurship. It has been seen that most Indian female entrepreneurs are successful in their businesses. These females are engaged in banking, manufacturing, food and beverage, electronic, textile, and small industries. However, the Indian government is trying to motivate female entrepreneurs in the handicraft industry, but there is less number of women entrepreneurship in these sectors due to many factors. Just a handful has benefited through initiative, especially in the handicraft industry. Furthermore, only a few research articles have focused on the effect of essential elements. The research provides an Entrepreneurship Event Model, a Theory of Planned Behaviour, and an integrated model of the theory, which is evaluated using data from some Indian Institutions focused on entrepreneurship.

Methane gas hydrate related bottom-simulating reflectors (BSRs) are imaged based on the in-line and cross-line multi-channel seismic (MCS) data from the Andaman Forearc Basin. The depth of the BSR depends on pressure and temperature and pore water salinity. With these assumptions, the BSR depth can be used to estimate the geothermal gradient (GTG) based on the availability of in-situ temperature measurements. This calculation is done assuming a 1D conductive model based on available in-situ temperature measurement at site NGHP-01-17 in the study area. However, in the presence of seafloor topography, the conductive temperature field in the subsurface is affected by lateral refraction of heat, which focuses heat in topographic lows and away from topographic highs. The 1D estimate of GTG in the Andaman Forearc Basin has been validated by drilling results from the NGHP-01 expedition. 2D analytic modeling to estimate the effects of topography is performed earlier along selected seismic profiles in the study area. The study extended to estimate the effect of topography in 3D using a numerical model. The corrected GTG data allow us to determine GTG values free of topographic effect. The difference between the estimated GTG and values corrected for the 3D topographic effect varies up to ∼5 °C/km. These conclude that the topographic correction is relatively small compared to other uncertainties in the 1D model and that apparent GTG determined with the 1D model captures the major features, although the correction is needed prior to interpreting subtle features of the derived GTG maps.

During the Indian National Gas Hydrate Program (NGHP) Expedition 02, Logging-while-drilling (LWD) logs were acquired at three sites (NGHP-02-11, NGHP-02-12, and NGHP-02-13) across the Mahanadi Basin in area A. We applied rock physics theory to available sonic velocity logs to know the distribution of gas hydrate at site NGHP-02-11 and NGHP-02-13. Rock physics modeling using sonic velocity at well location shows that gas hydrate is distributed mainly within the depth intervals of 150–265 m and 100–215 mbsf at site NGHP-02-11 and NGHP-02-13, respectively, with an average saturation of about 4% of the pore space and the maximum concentration of about 40% of the pore space at 250 m depth at site NGHP-02-11, and at site NGHP-02-13 an average saturation of about 2% of the pore space and the maximum concentration of about 20% of the pore space at 246 m depth, as gas hydrate is distributed mainly within 100–246 mbsf at this site. Saturation of gas hydrate estimated from the electrical resistivity method using density derived porosity and electrical resistivity logs from Archie's empirical formula shows high saturation compared to that from the sonic log. However, estimates of hydrate saturation based on sonic P-wave velocity may differ significantly from that based on resistivity, because gas and hydrate have higher resistivity than conductive pore fluid and sonic P-wave velocity shows strong effect on gas hydrate as a small amount of gas reduces the velocity significantly while increasing velocity due to the presence of hydrate. At site NGHP-02-11, gas hydrate saturation is in the range of 15%–30%, in two zones between 150-180 and 245–265 mbsf. Site NGHP-02-012 shows a gas hydrate saturation of 20%–30% in the zone between 100 and 207 mbsf. Site NGHP-02-13 shows a gas hydrate saturation up to 30% in the zone between 215 and 246 mbsf. Combined observations from rock physics modeling and Archie’s approximation show the gas hydrate concentrations are relatively low (

Introduction: Soil salinity poses a severe threat to rice production, resulting in stunted growth, leaf damage, and substantial yield losses. This study focuses on developing an early maturing seedling stage salinity tolerant rice variety by integrating conventional breeding methods with marker assisted breeding (MAB) approaches.Methods: Seedling-stage salinity tolerance Quantitative Trait Locus (QTL) “Saltol” from the salt-tolerant parent FL478 was introduced into the high-yielding but salt-sensitive rice variety ADT 45. This was achieved through a combination of conventional breeding and MAB. The breeding process involved rigorous selection, screening, and physiological parameter assessments.Results: KKL(R) 3 (KR 15066) identified as the top performing Recombinant Inbred Line (RIL), consistently demonstrating maximum mean grain yields under both salinity (3435.6 kg/ha) and normal (6421.8 kg/ha) conditions. In comparison to the early maturing, salt-tolerant national check variety CSR 10, KKL(R) 3 exhibited a substantial yield increase over 50%.Discussion: The notable improvement observed in KKL(R) 3 positions it as a promising variety for release, offering a reliable solution to maximize yields, ensure food security, and promote agricultural sustainability in both saline and non-saline environments. The study highlights the effectiveness of MAB in developing salt-tolerant rice varieties and emphasizes the significance of the Saltol QTL in enhancing seedling stage salinity tolerance. The potential release of KKL(R) 3 has the capacity to revolutionize rice production in salt affected regions, providing farmers with a reliable solution to maximize yields and contribute to food security while ensuring agricultural sustainability.

Trustworthiness, accuracy and believability are the most common indicators of news credibility measures. The impact of selective exposure, i.e. audience choosing content similar to their beliefs, on News Credibility is generally not considered in this context. This paper suggests that perceptions of News Credibility are influenced by selective exposure. This means people judge attitude-consistent content and sources as more credible. One explanation for selective exposure is ‘Motivated Skepticism’: we define a five-item construct for this construct. We hypothesize a Structural Equation Model with the latent construct Motivated Skepticism as a formative factor for News Credibility estimation of Indian television news channels. An online survey of 351 respondents from two Indian cities measures the goodness-of-fit and construct validity of the hypothesized SEM model. News Credibility is reported as a two- factor, second-order structure measured by Message Attributes and Channel Attributes, with Motivated Skepticism as a formative factor.

Abstract The COVID-19 pandemic had a significant impact on the global economy. For small- and medium-sized businesses, particularly in the handicraft sectors, it was exceptionally difficult to maintain operations during a global crisis. Without crisis management plans in place, business owners run the risk of having their operations abruptly terminated. Therefore, the researchers sought to comprehend and study the factors that led to the shutdown and departure strategies of microenterprises (in the handicraft sector) during the pandemic. To conduct this study, a descriptive research design was used, and registered Indian micro-businesses in the Cavite region were specifically chosen. According to the study's findings, the majority of microenterprises were sole proprietorships with a capitalization of less than INR 600,000.00 (Asian Development Bank in The COVID-19 impact on Philippine businesses: key findings from the enterprise survey. http://dx.doi.org/10.22617/SPR200214-2 , 2020) and had been in business for three to six years. As a result, the respondents' most typical issues are Retooling and training of pandemic-sensitive enterprise strategies for micro-entrepreneurs, particularly in the handicraft sector, as well as research into the negative effects of lockdown on MSME, which are all examples of crisis-sensitive interventions among small industries (handicraft sector) entrepreneurs.

Pollinators play a vital role in sustaining both cultivated and wild plants, thus ensuring the well-being of humanity. Unfortunately, the indiscriminate methods employed in pest-management have a detrimental impact on beneficial fauna, especially pollinators. Taking into account the harmful effects of pesticides on pollinators and the lack of explicit consideration for their welfare in Integrated Pest Management (IPM) practices, there is an urgent need to integrate pollinator management into the IPM programme. Incorporating pollinator management within the IPM framework is essential for maintaining the health of pollinators, preserving diverse pollinator species and achieving optimal crop yields with reduced pest-related issues- aspects that are often overlooked in conventional pest management approaches. This transformation from IPM to IPPM (Integrated Pest and Pollinator Management) highlights the importance of selectively and judiciously employing pesticides and other pest control strategies that have minimal impact on the habits, habitats, reproductive capabilities, foraging behaviour, growth, development and survival of pollinators. Although the concept of IPPM was introduced by Biddinger and Rajote (2015), our focus lies in explaining its relevance within the Indian context. Here, IPPM is imperative for reducing pest populations below economically damaging thresholds while simultaneously enhancing and conserving pollinators. By achieving this delicate balance, the ecological equilibrium can be preserved, ensuring the continued existence of various species and sustainability of our ecosystem.

This review paper addresses the escalating operation and maintenance costs of nuclear power plants, primarily attributed to rising labor costs and intensified competition from renewable energy sources. The paper proposes a paradigm shift towards a technology-centric approach, leveraging mobile and automated robots for physical security, aiming to replace labor-intensive methods. Focusing on the human–robot interaction principle, the review conducts a state-of-the-art analysis of dog robots’ potential in infrastructure security and remote inspection within human–robot shared environments. Additionally, this paper surveys research on the capabilities of mobile robots, exploring their applications in various industries, including disaster response, exploration, surveillance, and environmental conservation. This study emphasizes the crucial role of autonomous mobility and manipulation in robots for diverse tasks, and discusses the formalization of problems, performance assessment criteria, and operational capabilities. It provides a comprehensive comparison of three prominent robotic platforms (SPOT, Ghost Robotics, and ANYmal Robotics) across various parameters, shedding light on their suitability for different applications. This review culminates in a research roadmap, delineating experiments and parameters for assessing dog robots’ performance in safeguarding nuclear power plants, offering a structured approach for future research endeavors.

Plant defensins including Medicago Sativa defensin 1 (MsDef1) are cysteine-rich antifungal peptides which are known for potent broad-spectrum antifungal activity against bacterial or fungal pathogens of plants. The antimicrobial activities of these cationic defensins are attributed to their capacity to bind to cell membranes to create potentially structural defects tin the cell membranes to interact with intracellular target (s) and mediates cytotoxic effects. Our earlier work identified Glucosylceramide (GlcCer) of fungus F. graminearum as a potential target for biological activity. Multi-drug resistant (MDR) cancer cells overexpress GlcCer on the surface of plasma membrane. Hence, MsDef1 may have a potential to bind to GlcCer of MDR cancer cells to induce cell death. We have characterized the three-dimensional structure of MsDef1 and the solution dynamics using of 15N-labeled MsDef1 nuclear magnetic resonance (NMR) spectroscopy which showed that GlcCer binds MsDef1 at two specific sites on the peptide molecule. The ability of MsDef1 to permeate MDR cancer cells was demonstrated by measuring the release of apoptotic ceramide in drug resistant MCF-7R cells. It was also shown that MsDef1 activated dual cell death pathways ceramide and Apoptosis Stimulating Kinase ASK1 by disintegrating GlcCer and oxidizing tumor specific biomarker thioredoxin (Trx) respectively. As a result, MsDef1 sensitizes MDR cancer cells to evoke a better response from Doxorubicin, a front-line chemotherapy for triple negative breast cancer (TNBC) treatment. The combination of MsDef1 and Doxorubicin induced 5 to10-fold greater apoptosis in vitro MDR cells MDA-MB-231R compared to either MsDef1 or Doxorubicin alone. Confocal microscopy revealed that MsDef1 facilitates a) influx of Doxorubicin in MDR cancer cells, b) preferential uptake by MDR cells but not by normal fibroblasts and breast epithelial cells (MCF-10A). These results suggest that MsDef1 targets MDR cancer cells and may find utility as a neoadjuvant chemotherapy. Hence, the extension of antifungal properties of MsDef1 to cancer my result in addressing the MDR problems in cancer.

Objectives: Endometrial carcinoma (EC), majority being endometroid endometrial carcinoma (EEC), is the most common invasive gynecological cancer world-wide. Epithelial mesenchymal transition (EMT) is the process that cells undergo to switch from a polarized epithelial phenotype to a motile mesenchymal phenotype. Loss of E-cadherin and expression of N-cadherin are a critical step for development and progression of malignant tumors. In present study we evaluated the immunohistochemical (IHC) expression of E-cadherin and N-cadherin with clinicopathological parameters of EEC and its precursors lesions. Methods: It was a prospective nested case-control study involving women aged 35–70 years, whose endometrial biopsy were obtained for histological diagnosis. Signed informed consent was obtained before enrolling the women in the study, and the approval to conduct the study was obtained from the institutional ethics committee. The IHC was done to measure the E-cadherin and N-cadherin. Results: Reduced expression of E-cadherin was observed in 69% of cancer and 65% of precancer, however only 32% of normal proliferative endometrium showed reduced E-cadherin expression. N-cadherin expression was observed in 82% of EEC and 81% of precancer cases, while 11% of normal proliferative endometrium showed N-cadherin expression. Statistically significant downregulation of E-cadherin and upregulation of N-cadherin were observed in EEC and precancerous lesions as compared to normal control. Conclusion: Since downregulation of E-cadherin was also associated with upregulation of N-cadherin, the integration of immuno-expression of both E-cadherin and N-cadherin might offer a potential therapeutic target for EEC.

Abstract Background Tuberculosis currently stands as the second leading cause of deaths worldwide due to single infectious agent after Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). The current challenges of drug resistance in tuberculosis highlight an urgent need to develop newer anti-mycobacterial compounds. In the present study, we report the serendipitous discovery of a bacterial laboratory contaminant (LC-1) exhibiting a zone of growth inhibition on an agar plate seeded with Mycobacterium tuberculosis. Results We utilized microbiological, biochemical and biophysical approaches to characterize LC-1 and anti-mycobacterial compound(s) in its secretome. Based on 16S rRNA sequencing and BIOLOG analysis, LC-1 was identified as Staphylococcus hominis, a human bacterial commensal. Anti-mycobacterial activity was initially found in 30 kDa retentate that was obtained by ultrafiltration of culture filtrate (CF). SDS-PAGE analysis of peak fractions obtained by size exclusion chromatography of 30 kDa retentate confirmed the presence of high molecular weight (≥ 30 kDa) proteins. Peak fraction-1 (F-1) exhibited inhibitory activity against M. bovis BCG, but not against M. smegmatis, E. coli and S. aureus. The active fraction F-1 was inactivated by treatment with Proteinase K and α-chymotrypsin. However, it retained its anti-mycobacterial activity over a wide range of heat and pH treatment. The anti-mycobacterial activity of F-1 was found to be maintained even after a long storage (~12 months) at − 20 °C. Mass spectrometry analysis revealed that the identified peptide masses do not match with any previously known bacteriocins. Conclusions The present study highlights the anti-mycobacterial activity of high molecular weight protein(s) present in culture filtrate of LC-1, which may be tested further to target M. tuberculosis. The heat and pH stability of these proteins add to their characteristics as therapeutic proteins and may contribute to their long shelf life. LC-1 being a human commensal can be tested in future for its potential as a probiotic to treat tuberculosis.

Introduction: Extranodal Non-Hodgkin’s Lymphoma (NHL) constitutes approximately one fourth of all NHL. The data regarding extranodal NHL from India is however limited. Aim: To evaluate the epidemiological and histomorphological patterns of extranodal NHL in a tertiary care institute in Northern India. Materials and Methods: The retrospective observational study was conducted on data of biopsy sections retrieved from the archives for a period of four years (January 2016 to December 2019), 130 extranodal NHL cases (90 males, 40 females) that were analysed for histological features and immunohistochemical subtype. All statistical analysis was done by Statistical Package for Social Sciences (SPSS) software. The values were represented in number, percentage, mean and ratio. Results: Total of 362 cases of NHL were reported. Out of which, 130 (36%) cases were of extranodal NHL. Male to female ratio being was 2.25:1. Mean age of presentation was 38.5 years. Paediatric cases constituted (32/130; 24.6%) cases. Head and neck was the most common site (52/130; 40%) followed by Gastrointestinal Tract (GIT) (21/130; 16.2%) and central nervous system (13/130; 10%). Amongst the paediatric cases, head and neck (11/32; 34.4%) was the most common site, followed by GIT (8/32; 25%). Amongst head and neck lymphomas, nasal cavity and Waldeyer Ring were the most common sites followed by orbit and oral cavity. Common sites among the GIT lymphomas were stomach, small intestine and large intestine. The B-cell NHL constituted 81/130 (62.3%), T-cell NHL 20/130 (15.4%) and NHL (Unclassified) were 29/130 (22.3%). Amongst B-cell type NHL, Diffuse Large B Cell Lymphoma (DLBCL) was the most common subtype. Conclusion: Extranodal NHL diagnosis is challenging due to morphological similarities with non haematopoietic tumours. Lineage determination by Immunohistochemistry (IHC) serves as the key to diagnosis. In our population, head and neck was the most common site, with B-cell NHL being the most common subtype.

The Tuaheni Landslide Complex, located on the upper slope of the northern Hikurangi Margin in New Zealand, is a unique place to research on slow slip creep-like deformation and seabed failure, as well as their possible relationship with the presence of gas hydrates, cold seeps, and fluid migration. Based on the visual interpretation of seismic data, it is sometimes very difficult to identify various subsurface structures and tectonic features. We study certain seismic attributes, namely the reflection strength, instantaneous frequency, instantaneous phase, and the Hilbert transform, in the Tuaheni Landslide Complex and observe that these attributes play a very important role in identifying and interpreting various subsurface geological features and bed boundaries that are not clearly visible in the seismic sections. In general, these seismic attributes are studied to identify hydrocarbons such as oil and gas. However, in this present study these seismic attributes nicely illustrate the fluid migration pathways, the decollement of the sediment slide, the base of the debris flow, the base of the deformed sediment and gas migration, etc., along two perpendicular seismic profiles crossing the Site U1517 of IODP Expedition 372. The instantaneous phase and Hilbert transform attribute depict the bed boundaries and discontinuities, whereas the reflection strength and instantaneous frequency attributes characterize the various strata in terms of whether they are associated with fluid at their bases. The possible role of tectonic activity and seafloor slope failure due to gas hydrate dissociation and vice versa is clearly visible through fluid-filled weak zones in the seismic attribute volumes. Gas hydrates are dissociating and BSRs are abruptly pinching out towards the seafloor due to the movement of hot fluid and free gas, enhancing seafloor sliding and local tectonic activities together.

Zika virus (ZIKV), a mosquito-transmitted Flavivirus, emerged in the last decade causing serious diseases and affecting human health globally. Currently, no licensed vaccines or antivirals are available to combat ZIKV, although several vaccine candidates are in the pipeline. In recent years, the presence of non-canonical G-quadruplex (GQ) secondary structures in viral genomes has ignited significant attention as potential targets for antiviral strategy. In this study, we identified several novel conserved potential GQ structures by analyzing published ZIKV genome sequences using an in-house algorithm. Biophysical and biochemical analysis of the RNA sequences containing these potential GQ sequences suggested the existence of such structures in the ZIKV genomes. Studies with known GQ structure-binding and -stabilizing ligands such as Braco-19 and TMPyP4 provided support for this contention. The presence of these ligands in cell culture media led to significant inhibition of infectious ZIKV yield, as well as reduced viral genome replication and viral protein production. Overall, our results, for the first time, show that ZIKV replication can be inhibited by GQ structure-binding and -stabilizing compounds and suggest a new strategy against ZIKV infection mitigation and control.

Background: In the wake of the unprecedented Mucormycosis epidemic following the delta variant run second wave of SARS COV-2, the Government of Telangana decided to utilize Ayurveda for its adjuvant immune-boosting role. Based on clinical assessment of patients, Mucor mycosis was identified as Vataja vidradhi and treatment was planned. Objective: To assess the safety and efficacy of Ayurvedic regime as an adjuvant therapy in post covid mucor mycosis patients at Gandhi Hospital, the largest Government tertiary care center in Telangana. Methods: In this prospective case control study, 77 patients with positive or probable post covid mucor mycosis were included. The varunadi kwatha regimen could be given in very few patients precluding its meaningful analysis. 65 patients received Pancha Tikta Ghrita Guggulu [PTGG] regimen. These patients were assigned into 2 groups; Intervention [A] group n = 36, who used PTGG for a mean duration of 34.1 days and Control [B] group [drop outs] n = 29 who used PTGG for a mean duration of 2.1 days. Objective parameters like biochemical changes and parameters like disease progression, recurrence, mortality rate, subjective and objective wellbeing, readmission, repeat surgeries, and persistence of symptoms were assessed before and after treatment. Statistical analysis was done using Mann Whitney, chi square and ANOVA test. Results: The Intervention group mortality rate was ZERO where as it was 13.8% in control group. The statistical analysis showed significant improvement in all clinical parameters tested. Conclusion: PTGG based regimen as adjuvant seemed to help across the entire spectrum of Mucormycosis. It was safe and tolerated very well with concomitant antifungal usage and in pre and post-operative patients, thus validating the host factor modification approach of Ayurveda and an integrative usage.

This study presents a systematic review of the literature on virtual reality (VR), augmented reality (AR) and Mixed Reality (MR) used in disaster management. We consider the factors such as publication type, publication year, application domain, and technology used. We surveyed papers from 2009 to 2019 available in the Web of Science and Google Scholar database, and 84 research articles were selected for the review study. After an extensive review of the literature, it was found that the XR technology is applied extensively in computer simulation modeling, interaction techniques, training, infrastructure assessment and reconnaissance, and public awareness areas of disaster management. We found diverse advantages, opportunities, and challenges of XR usage for disaster management, which are discussed in detail. Furthermore, current research gaps in the field of XR technology for disaster management technology, which are needed to better support disaster management, are identified and discussed in an effort to provide direction to the future research.

Introduction: Cyclin D1 is a protein encoded by the CCND1 (BCL-1) gene on chromosome 11q13 and it is an important regulator of G1 to S phase progression. Over expression of cyclin D1 protein releases cells from their normal controls when they need to exit from the cell cycle. This obstructs their maturation, and promotes transformation into a malignant phenotype. Aim: To study the role of cyclin D1 expression in trephine biopsies of multiple myeloma patients and its association with C-Reactive Protein (CRP), β2-microglobulin level and treatment response rate. Materials and Methods: This prospective observational study was conducted at Department of Pathology in collaboration with Department of Clinical Haematology, King George’s Medical University, Lucknow, India, from September 2018 to August 2019. Total 40 cases of multiple myeloma fulfilling inclusion and exclusion criteria were enrolled. Bone marrow aspiration and biopsy was done in all the cases. Immunohistochemical (IHC) expression of cyclin D1 on trephine biopsy was associated with CRP levels and β2-microglobulin expression. All investigations were repeated at six months follow-up and response was compared with expression of cyclin D1. The statistical tests applied were Chi-square test, Student t-test and paired t-test. Results: The age of cases ranged between 44 to 78 years and the mean age of the study subjects was 64.40±7.13 years. Total 67.5% of patients were males. On IHC, cyclin D1 expression was not observed in majority of cases (n=23), weak cyclin D1 expression was observed in 8 cases, while strong cyclin D1 expression was observed in 9 cases. Out of eight cases with weak cyclin D1 expression, five cases achieved partial response and two cases achieved complete response. One case was lost to follow-up. Among nine patients with strong cyclin D1 expression, six patients expired on six months follow-up and three patients achieved partial response. On comparison of two groups cyclin D1 positive and cyclin D1 negative cases it was found that cyclin D1 positive cases had an early age of onset, more than 50% plasma cells on marrow aspirate and were associated with plasmablastic morphology. Cyclin D1 positive cases also had increased CRP level as compared to cyclin D1 negative cases. Similarly, serum calcium, serum creatinine and β2-microglobulin levels were more in cyclin D1 positive group. Conclusion: Cases who have strong cyclin D1 expression at time of diagnosis showed poor response to treatment. This was also associated with increased serum CRP and β2-microglobulin levels. Hence, cyclin D1 can be used as a prognostic marker in multiple myeloma.

Epidemics have not been new to India. Ayurveda being in the fore front of health care of those times, has recorded its valuable experiences of epidemics & termed them as Janapadodhwamsa or Maraka. It has vividly described their mechanism of Causation (Nidana), factors affecting severity and actions complicating illnesses their management and prevention. The present work uses the same model described in Samhitas which assert the superiority of host susceptibility (Nija) over External Agent/Virus (Agantuja) and uses the same principles in prevention; while adopting the Jwara model to explain and correlate with different stages of COVID – 19. It proposes management guidelines using Ahara (Nutritional Principles) and Oushadha (Medication) for different stages and subsets of patients along with their potential complications and drug interactions, utilizing the principles of host factor modification (Dosha hara) and potential broad spectrum Anti-viral (Prativisha) as envisaged by Rasa Shastra.

Mycobacterium tuberculosis (Mtb) is an intracellular pathogen that forms aggregates (clumps) on solid agar plates and in liquid media. Detergents such as Tween 80/Tyloxapol are considered the gold standard to disrupt clump formation in Mtb cultures. The presence of detergent, however, may generate foam and hinder Mtb aerosolization thus requiring addition of an antifoam agent for optimal Mtb aerosol-based procedures. Aerosol inhalation can be technically challenging, in particular to achieve a reproducible inhaled target dose. In this study, the impact of an antifoam, the silicon antifoaming agent (SAF), on Mtb aerosolization and whole-body mouse aerosol infection was investigated. A comparative study using SAF in a liquid suspension containing Mycobacterium bovis BCG (M. bovis BCG) or Mtb H37Rv did not cause any adverse effect on bacterial viability. Incorporation of SAF during mycobacteria inhalation procedures revealed that aerosolized mycobacterial strains were maintained under controlled environmental conditions such as humidity, temperature, pressure, and airflow inside the aerosol chamber. In addition, environmental factors and spray factors were not affected by the presence of SAF in mycobacterial cultures during aerosolization. Spray factor was significantly less during aerosol procedures with a low-input dose of mycobacteria in comparison to high-dose, as predicted. The mycobacterial load recovered in the biosampler (AGI) was ~2–3 logs lower than nebulizer or input bacterial load. A consistent Mtb bacillary load determined in mouse lungs indicates that SAF does not affect mycobacteria aerosolization during the aerosol generation process. These data confirmed that 1) SAF prevents formation of excessive foam during aerosolization, 2) SAF had no negative impact on mycobacterial viability within aerosol droplets, 3) Mtb droplets within aerosol-generated particles are well within the range required for reaching and depositing deep into lung tissue, and 4) SAF had no negative impact on achieving a target dose in mice exposed to Mtb aerosol.

It appears that the perception and desire to utilize recycled aggregate in concrete is growing across the world, decreasing the environmental impact of landfills and virgin aggregate mining while also making building projects more cost-effective and environmentally friendly. However, the practical utilization of RA has not gained attention to date because of the weaker characteristics of porous attached mortar. Although various mix designs for recycled aggregate concrete (RAC) have been proposed earlier by many authors, still using RA is not made practical for many reasons. One of them is the unavailability of an easy and effective design mix methodology using RAC. Thus, an attempt has been made in the present investigation to advance a mix design methodology for RAC by providing w/b to strength relation, embracing the two-stage mixing approach (TSMA) by completely substituting the CNA with CRA. DIN standard was followed to get the maximum particle packing of aggregates and improved RAC performance. Again, to make RAC more sustainable, a mix design is also proposed to replace cement with widely used SCMs-coal fly ash (CFA) and ground granulated blast furnace slag (GGBS) at 30 and 50% replacement level, respectively. The synergetic effect of the proposed design mix that contains DIN standards, TSMA, and SCMs on fresh, hardened properties, and evaporation studies of RAC was studied. In this context, the comparative analysis manifested the encouraging outcomes to use the proposed mix design methodology for preparing RAC incorporated with CFA and GGBS.

The main focus of this research has been on examining the renewable energy sources (RESs) consumption with respect to green consumer values (GCVs). These GCVs have primarily been examined with regard to different products and services. The most significant strategy for strengthening everyone is social education. While GCVs are assumed to be nurtured through education, specific understanding about the long-term benefits of renewable energy for a sustainable life is measured with the belief that the greater the training and development the better shaped the society can be, which boosts morale and increases their energy reliance with RES, assessed by the respondents of Uttar Pradesh (UP), as the outcome of the research. The implications for the stakeholders are the long-term advantages of renewable energy for a sustainable existence, since a society may be better formed and its reliance on RESs for energy rises the more training and development it receives. The advantages are visible, though we may not be able to completely transition soon, but it is crucial that we think for the future. The quantitative research method applies statistical analyses, such as ANOVA, spectral analysis, and multilayer perceptron analysis, justifying the requirement of education. The results justify that the solution to this problem—that green consumer values are being learned—can be nurtured by societal education. Learning about green consumer values can make society understand the renewable energy benefits, which can better shape society and lead to capacity building.

The energy sector is one of the most important sectors as it is the producer and supplier of fuel to run other industries and economies. This research aims to present an intertwined structure of risk exposure measuring the performance of the S&P Bombay Stock Exchange (BSE) Energy Index and selected companies from the constituents while taking into account two scenarios of risk, namely the COVID-19 pandemic and the Russia–Ukraine conflict, in a manner appropriate for energy stock investors, energy companies, and the economy through hedging against investment risk, diversification in operations securing the continuation of energy production, and the risk of fluctuating prices in the energy market, respectively. The research problem is observed as the requirement to choose the representative stocks of the S&P (BSE) Energy Index to evaluate their situations during the period of the two crisis scenarios and to provide the current risk exposure to India’s energy sector. The methodological approach was through the process of selecting the stock market, the reason behind selecting the energy sector, analyzing the crisis situation, and finally providing the risk exposure matrix. The COVID-19 pandemic affected the index and stocks only in the beginning when the market was scared psychologically. The Russia–Ukraine conflict is considered to measure the stock status showing the effect on the index and the effect on selected stocks showing a deviated performance. All ten companies representative of the S&P BSE Energy Index ranked in the increasing order of risk exposure comparatively and concludes a high potential growth and return.

Background: In India, the magnitude of the problem of intellectual disability (ID) hardly needs any exaggeration. In India, the prevalence varies from 0.63% to 7.14%. Depending on the severity of the ID, children tend to be proportionately dependent on their parents. Aim: The aim is to know the disability impact and the type of impact of having an ID child on the parents. Materials and Methods: Hundred parents of ID children attending the outpatient department (OPD) services either for treatment for behavioral issues or rehabilitation services to child psychiatry OPD of tertiary care hospital. The level of ID is assessed using developmental screening test and the International Classification of Diseases-10. The disability impact on parents is assessed using the modified National Institute of Mentally Handicapped Disability Impact Scale (DIS). Results: 56.63% of parents have disability impact as related to the physical care of the child, financial problems (58.87%), loss of support from relatives and inlaws, and maintaining social relations with friends and neighbors due to social restriction (36.75%) domains of DIS. As the severity of ID increased, disability impact concerning physical care is also increased along with specific negative thoughts. Majority of parents (71.72%) did not feel any kind of embarrassment because of the ID child. 67.3% of the parents had a positive impact like improved tolerance and patience. Conclusions: The current study gives the heartening finding that most of the parents of the selected intellectually disabled children viewed that they have a more positive impact of 67.3% which implies that every family with a disabled child will not have a negative impact nor it is a bad fate or misfortune. In many families, it can have a positive impact like increased Self Efficacy, parental and family general satisfaction with life which helps the parents to provide necessary care and support and handle the situation more gracefully.

Stratification in lowland rainforests of Meghalaya, India, which represent the westernmost limit of the rainforests north of the Tropic of Cancer, was studied in horizontal and vertical planes to elucidate the patterns in stacking of species diversity and community attributes, and to draw comparisons with rainforests of ‘Indo-Malaya’ ecozone (biogeographical realm). All individuals ≥10 cm GBH (girth at breast height) were enumerated in six transects of 10 m width and up to 500 m length covering 2.45 ha area. The stratification of whole assemblage of species in vertical plane is referred to as ‘storey structure’ (=height class distribution), which explains structural complexity. In horizontal plane, it is typically referred to as ‘stand structure’ (=girth class distribution), which explains structural heterogeneity. The stratification of an individual species in vertical plane is referred to as ‘loftiness’ and in horizontal plane, it is frequently referred to as ‘population structure’. The stand structure was characterized by a negative exponential relationship or a reverse J-shaped curve, which is typical of a well regenerating forest stand. The storey structure was characterized by the low stature of the rainforest (

G-quadruplex structure forming motifs are among the most studied evolutionarily conserved drug targets that are present throughout the genome of different organisms and susceptible to influencing various biological processes. Here we report highly conserved potential G-quadruplex motifs (PGQs) in three essential genes (espK, espB, and cyp51) among 160 strains of the Mycobacterium tuberculosis genome. Products of these genes are involved in pathways that are responsible for virulence determination of bacteria inside the host cell and its survival by maintaining membrane fluidity. The espK and espB genes are essential players that prevent the formation of mature phagolysosome and antigen presentation by host macrophages. The cyp51 is another PGQ-possessing gene involved in sterol biosynthesis pathway and membrane formation. In the present study, we revealed the formation of stable intramolecular parallel G-quadruplex structures by Mycobacterium PGQs using a combination of techniques (NMR, circular dichroism [CD], and gel electrophoresis). Next, isothermal titration calorimetry (ITC) and CD melting analysis demonstrated that a well-known G-quadruplex ligand, TMPyP4, binds to and stabilizes these PGQ motifs. Finally, polymerase inhibition and qRT-PCR assays highlight the biological relevance of PGQ-possessing genes in this pathogen and demonstrate that G-quadruplexes are potential drug targets for the development of effective anti-tuberculosis therapeutics. Keywords: G-quadruplex, Mycobacterium tuberculosis, drug targets, anti-tuberculosis compounds, G-quadruplex mediated inhibition, G-quadruplex binder

Renewable energy consumption is the call by United Nation Sustainable Development Goals, and sustainable consumption is the only solution for the future. This study found that the solution to the framed problem has a requirement to categorize the green consumer value parameters in hierarchy of importance for five specific RESs and their alignment with environment policy based on a comparison of Poland and India. The study revealed the importance of each criterion for renewable energy sources providing, a comparative table of Poland and India. The methodological approach used secondary data for selecting countries, and primary data are used for statistical analysis of Automatic Linear Modelling. The research concludes that the CRITERIA4 is one of the most important that emerged with all five RESs for Poland and three RESs for India. Both countries are aligned with the UN sustainable development goals and are switching over to all means of sustainability. Poland scores a higher value of importance compared to India, which justifies the awareness of consumers.

BackgroundAs per national policy, all diagnosed tuberculosis patients in India are to be tested using Xpert® MTB/RIF assay at the district level to diagnose rifampicin resistance. Regardless of the result, samples are transported to the reference laboratories for further testing: first-line Line Probe Assay (FL-LPA) for rifampicin-sensitive samples and second-line LPA(SL-LPA) for rifampicin-resistant samples. Based on the results, samples undergo culture and phenotypic drug susceptibility testing. We assessed among patients diagnosed with tuberculosis at 13 selected Xpert laboratories of Karnataka state, India, i) the proportion whose samples reached the reference laboratories and among them, proportion who completed the diagnostic algorithm ii) factors associated with non-reaching and non-completion and iii) the delays involved.MethodsThis was a cohort study involving review of programme records. For each TB patient diagnosed between 1st July and 31st August 2018 at the Xpert laboratory, we tracked the laboratory register at the linked reference laboratory until 30th September (censor date) using Nikshay ID (a unique patient identifier), phone number, name, age and sex.ResultsOf 1660 TB patients, 1208(73%) samples reached the reference laboratories and among those reached, 1124(93%) completed the algorithm. Of 1590 rifampicin-sensitive samples, 1170(74%) reached and 1104(94%) completed the algorithm. Of 64 rifampicin-resistant samples, only 35(55%) reached and 17(49%) completed the algorithm. Samples from rifampicin-resistant TB, extra-pulmonary TB and two districts were less likely to reach the reference laboratory. Non-completion was more likely among rifampicin-resistant TB and sputum-negative samples. The median time for conducting and reporting results of Xpert® MTB/RIF was one day, of FL-LPA 5 days and of SL-LPA16 days.ConclusionThese findings are encouraging given the complexity of the algorithm. High non-reaching and non-completion rates in rifampicin-resistant patients is a major concern. Future research should focus on understanding the reasons for the gaps identified using qualitative research methods.

Vibrio cholerae, a gram-negative bacterium that causes cholera, has already caused seven major pandemics across the world and infects roughly 1.3–4 million people every year. Cholera treatment primarily involves oral rehydration therapy supplemented with antibiotics. But recently, multidrug-resistant strains of V. cholerae have emerged. High genomic plasticity further enhances the pathogenesis of this human pathogen. Guanines in DNA or RNA assemble to form G-quadruplex (GQ) structures which have begun to be seen as potential drug targeting sites for different pathogenic bacteria and viruses. In this perspective, we carried out a genome-wide hunt in V. cholerae using a bio-informatics approach and observed ∼85 G-quadruplex forming motifs (VC-PGQs) in chromosome I and ∼45 putative G-quadruplexs (PGQs) in chromosome II. Ten putative G-quadruplex forming motifs (VC-PGQs) were selected on the basis of conservation throughout the genus and functional analysis displayed their location in the essential genes encoding bacterial proteins, for example, methyl-accepting chemotaxis protein, orotate phosphoribosyl transferase protein, amidase proteins, etc. The predicted VC-PGQs were validated using different bio-physical techniques, including Nuclear Magnetic Resonance spectroscopy, Circular Dichroism spectroscopy, and electrophoretic mobility shift assay, which demonstrated the formation of highly stable GQ structures in the bacteria. The interaction of these VC-PGQs with the known specific GQ ligand, TMPyP4, was analyzed using ITC and molecular dynamics studies that displayed the stabilization of the VC-PGQs by the GQ ligands and thus represents a potential therapeutic strategy against this enteric pathogen by inhibiting the PGQ harboring gene expression, thereby inhibiting the bacterial growth and virulence. In summary, this study reveals the presence of conserved GQ forming motifs in the V. cholerae genome that has the potential to be used to treat the multi-drug resistance problem of the notorious enteric pathogen.

An opportunistic pathogen, Klebsiella pneumoniae is known to cause life-threating nosocomial infection with a high rate of morbidity and mortality. Evolutions of multi-drug-resistant and hyper-virulent strains of K. pneumoniae make the situation worse. Currently, there is no incisive drug molecule available for drug-resistant hyper-virulent K. pneumoniae infection that emphasizes the need for identification of novel and more promising drug targets in K. pneumoniae. Recently, various non-canonical structures of nucleic acids especially G-quadruplex (G4) motifs have been identified as potential therapeutic targets against several human pathogenic bacteria and viruses including Mycobacterium tuberculosis, Streptococcus pneumoniae, human immunodeficiency virus (HIV), Ebola, and Nipah. Therefore, in present study we screened the K. pneumoniae genomes for identification of evolutionary conserved G4 structure-forming motifs as promising anti-bacterial drug targets. Bioinformatics analysis revealed the presence of six highly conserved G4 motifs in the promoter region of five essential genes that play a critical role in nutrient transport and metabolism. Biophysical studies showed the formation of G4 structure by these conserved motifs. Circular Dichroism melting analysis showed the stabilization of these G4 motifs by a well-known G4-stabilizing agent, BRACO-19. The stabilization of these motifs by BRACO-19 was also able to stop the primer extension process, which is an essential phenomenon for expression of the G4-harboring gene. The addition of G4-specific ligand at low micromolar range was observed to be lethal for the growth of this bacteria and negatively controlled the expression of the G4-harboring genes via G4 structure stabilization. These observations strengthen the formation of G4 structures by the predicted G4 motif in vivo, which can be stabilized by G4 ligands like BRACO-19. This stabilization of G4 structures can attenuate the expression of G4-harboring essential genes and thus play a critical role in the regulation of gene expression. Thus, taking all given result in consideration, for the first time, this study showed the new therapeutic avenue for combating K. pneumoniae infection by characterizing the conserved G4 motifs as promising therapeutic targets.

Mercury generates free radicals and subsequently increases oxidative stress, which leads to renal injury. Tribulus terrestris (TT) has good anti-inflammatory and antioxidant properties. Hydroalcoholic extract of different dose of TT was evaluated against mercuric chloride-induced nephrotoxicity. Rats (n = 6) were treated with TT at doses of 100, 200, and 300 mg/kg. Drugs were administered orally for 7 days. Single dose of mercuric chloride (5 mg/kg, intraperitoneal) on the 5th day caused significant elevation of blood urea nitrogen, serum creatinine, malondialdehyde, liver fatty acid binding protein, kidney injury molecule-1, and kidney mercury level and fall in glutathione, superoxide dismutase, glutathione peroxidase, and histopathological changes in disease control as compared to normal control group (P < 0.001). Dose of TT 200 and 300 mg/kg significantly (P < 0.001) prevented the renal injury, and mercury accumulation in kidney tissues significantly decreases in higher dose, i.e., 300 mg/kg as compared to control group. Our result indicates that the treatment of TT exerted significant protection against renal damage induced by mercuric chloride possibly due to its antioxidant and anti-inflammatory properties and by decreasing the renal accumulation of mercury.

This article examines the acceptance of renewable energy in the adoption of the climate package, taking into account the GREEN consumer values among consumers in Poland. The research problem addressed in this paper relates to the measurement of consumer values in terms of the GREEN concept; specifically, consumer acceptance of the available offerings provided by real businesses. In addition, consumer needs are important in terms of ensuring the acceptance of renewable energy, which will create opportunities for its production in the context of the Fit 55 climate package. The study formulates some research questions and hypotheses to justify the concept with empirical data by applying statistical tests. The study is presented in several sections with a detailed presentation of secondary and primary data. The main outcome of the research is based on the primary data using a structured survey questionnaire administered in Poland and 557 collected samples. Nowadays, the responsibility also lies with consumers, as green consumption will entail green production. The research concludes that Poland’s consumers have a good level of acceptance of RES, based on the current green energy situation of Poland, which may lead to sustainability and will benefit policy makers in government, corporations and end consumers.

Renewable energy sources are ecological and sustainable solutions accepted with a common consensus under the sustainable development goals. Energy source exploration has enforced the human race to think about sustainable energy production as fossil fuels are non-renewable and are having a negative impact on ecosystem. Consumer needs and wants are the key for acceptance of renewable energy and making production a possibility. The real challenge is with the consumer market for the acceptance of green energy. The current research is based on the concept of GREEN consumer values synergizing with renewable energy sources, and eco innovation for sustainability in India. The crucial point of study is assessing GREEN consumer values which are the determining factor for consumption. All five renewable energy sources (solar, wind, hydro, geothermal and biomass) have received a higher level of acceptance by respondents which shows that India is on its way to enhancing its capacity for renewable energy. Indian consumer values are very much aligned with eco sustainability, and the market is ready to consume renewable energy.

The study is descriptive in nature measuring the effect and relationship in between sales promotion activities and brand loyalty. The study is done on COCA COLA brand in the open market. The research problem observed here as to measure the efficiency of sales promotion and brand loyalty to get the real effectiveness on sales promotion activities. The research objectives formulated are to know the importance of component of sales promotion activities, to know the effect of advertising on sales promotion activities, to realize the importance of brand loyalty on sales promotion activities. A quantitative method was used to accomplish the purpose of the study. A survey instrument developed taking items to measure all variables with five points likert scale. A sample size of 159 (from 210) incorporated in the study having the reliability of 0.851(more than 0.7). The data analyzed using SPSS for descriptive statistics and regression to measure the effect of independent variable on dependent variable. It is recommend that COCA COLA should improve more on its different activities and produce different product and with tasty fast food or build up different production of equipment to expand its business in the whole world’s market .

Brand Image is one of the most vital parts of any organization that makes the world believe their products or services. It creates a pre assumption in the mind before reaching or consuming it. It is said too that the Lion has branded itself means the image established like creates the fear when one just gets an imagination. The different imagination of different brand is the outcome of its performance in the market. The study presented here in this study is the preliminary part of the deeper concept of Brand Image. Though, the study is the small part of the many broader dimensions explored by many researchers then too is the idea here to build the platform for the better understanding of the Brand Image in the context of university and academic institutions where many authors say academic institutions are not prone to have any exercise for building their Brand Image. The literature review is based on the idea of finding the Brand Image during past years when the concept was evolving and the current scenario. Conceptually the study has taken the literature across the world and across industries but the specific narrowed down discussion is carried for Kurdistan region.