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This study focuses on investigation the spatial distribution of pollutants in Çorlu Stream is the highest industrialized tributary of Ergene River which is one of the most polluted rivers in Turkey. A total of 250 conventional, metal and micropollutants were scanned at eleven sampling locations for four seasons in Çorlu Stream of Ergene River. At these locations, total of 126, 124, 99 and 107 pollutants were detected at least one sampling location in summer, fall, winter and spring, respectively. Four micropollutants which are associated with textile industry were detected in every location in all seasons. Cluster Analysis found four main clusters for sampling locations from most polluted to low polluted, and six cluster for pollutants ranging from conventional to unique pollutants. Principal Component Analysis identified six components for every season explaining more than 80% of the variation in each. PCA results indicated the impact of textile, leather, metal industries and appliance-electronics production in the Çorlu Region.

Contamination of agricultural soil with organic contaminants is a global problem due to the risks associated with food security and ecological sustainability. Besides the use of agrochemicals, hundreds of emerging contaminants enter arable lands through polluted irrigation water. In this study, an analytical workflow based on QuEChERS extraction coupled with LC-MS/MS quantification was applied to measure 65 emerging contaminants (42 pesticides and 23 multiclass industrial chemicals) in soil and rice for the first time. The method was validated on paddy and yard soil and rice plants. A recovery efficiency ranging between 70 and 120% (RSD

Currently about 110,000 chemical substances are present in the European market. The fate of most of those chemicals in the environment is not known. However, biodegradability of those chemicals should be tested before they are registered to the Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH) System. Current protocols offered by The Organisation for Economic Co-operation and Development (OECD) for testing the biodegradability of the chemicals are limited mainly due to they are low throughput and do not reflect real-world conditions. In OECD protocols, the biodegradability of a single chemical is tested. However, many chemicals coexist in the environment. In addition, experiments are set at a very high initial chemical concentration that is not expected in the environment. Both limitations are due to the lack of an analytical method which can measure multiple compounds simultaneously at very low concentrations. In this study, we coupled OECD 314 Simulation Tests to Assess the Biodegradability of Chemicals Discharged in Wastewater protocol with a powerful liquid chromatography mass spectrometry with scheduled multiple reaction monitoring and tested the biodegradability of 32 priority substances and chemicals with emerging concern. Only seven chemicals were degraded in the test within 28 days. The biodegradation half-lives of those degradable chemicals ranged between 0.6 to 18 days. Acetaminophen was degraded the fastest whereas biodegradation of sulfamethoxazole took longer than the rest of the biodegradable chemicals tested. The novel methodology described here can be applied to test biodegradability of different chemicals as a mixture and adopted as a standard protocol.

Dezenfektanaktif maddeleri kentsel ve endüstriyel atıksularda oldukça sık rastlanankirleticilerdir. Bu kirleticilerin biyolojik sistemlerde giderimi oldukçazordur. Yüzeysel sularda da sıkça karşılaşılan bu kirleticiler hem doğal hayatıhem de insan sağlığını tehdit etmektedir. Bu çalışmanın amacı, atıksuda en çok karşılaşılankirleticilerden biri olan benzalkonyum klorürlerin (BAK’ler) biyolojik sistemlerdeen verimli şekilde giderimini sağlayacak koşulların belirlenmesidir. Bu amaçla,atıksudan izole edilmiş BAK gideren bir bakteri olan Pseudomanas sp. BIOMIG1’infarklı koşullarda BAK biyotransformasyon kinetiği belirlenmiştir. Elde edilenveriler ve Michaelis-Menten modeli kullanılarak, bu mikroorganizmanın BAK biotransformasyonkinetiği parametreleri hesaplanmış ve uygulanan koşulların kinetiğe etkisibelirlenmiştir. BIOMIG1, BAK’leri 1.4 mg/L-saat hızında giderebilmekte ve bukirleticileri amonyak ve karbon dioksite dönüştürmektedir. Mililitrede yüz binadet bakteri yoğunluğu gibi düşük bakteri yoğunluklarında bile BAK gecikmeli deolsa yüksek hızda giderilebilmektedir. BAK homologlarının biyotransformasyonhızı karşılaştırıldığında, 14 karbon alkil zincir uzunluğuna sahip BAK enhızlı, 16 karbonlu BAK ise en yavaş biyotransformasyon hızına sahiptir. BAK giderimhızının en yüksek olduğu sıcaklık 35°, bu sıcaklık üstündeki sıcaklıklardaBIOMIG1 yaşayamamaktadır. Dolayısıyla yüksek sıcaklıklarda BAK parçalanması yabenzildimetilamin birikmesiyle sonlanmış ya da hiç gerçekleşmemiştir. Buçalışmanın sonuçları, özellikle BAK gibi mikrokirleticilerin arıtımınıhedefleyen ileri arıtma sistemlerinin tasarlanması ve işletilmesinde faydalıolacaktır., Activeingredients of disinfectants are very common pollutants in urban and industrialwastewater. Removal of these contaminants is very difficult in biologicaltreatment systems. As a result; these pollutants, which are also frequentlydetected in surface waters, threaten both nature and human health. Theobjective of this study is to determine the optimum conditions that willprovide the most efficient removal of benzalkonium chlorides (BACs), a commoncontaminant, in biological treatment systems. For this purpose, BACbiotransformation kinetics were determined under different conditions usingPseudomanas sp. BIOMIG1, a bacterium that is the key BAC degrader in theenvironment. Using the data collected and the Michaelis-Menten growth model,BAC biotransformation kinetic parameters were calculated and the effect of theapplied conditions on kinetics was determined. BIOMIG1 can transform BACs at arate of 1.4 mg/L-hour and converts these pollutants into ammonia and carbondioxide. BAC mineralization can be achieved even at low bacterial densitiessuch as 100000 cells/mL after a short delay. When biotransformation rate of BAChomologs was compared, BAC with 14 carbon alkyl chain length had the fastestand BAC with 16 carbons had the slowest rate of biotransformation. Thetemperature at which the BAC biotransformation rate was the highest was 35degrees. BAC was converted to benzyldimethylamine at all temperatures above 35degrees since BIOMIG1 does not survive above this temperature. The outcomespresented in this study would be used for the design and operation of advancedtreatment systems targetting the removal of micropollutants like BACs.

Quaternary ammonium compounds (QACs) are active ingredients of many disinfectants used against SARS-CoV-2 to control the transmission of the virus through human-contact surfaces. As a result, QAC consumption has increased more than twice during the pandemic. Consequently, the concentration of QACs in wastewater and receiving environments may increase. Due to their antimicrobial activity, high levels of QACs in wastewater may cause malfunctioning of biological treatment systems resulting in inadequate treatment of wastewater. In this study, a biocatalyst was produced by entrapping Pseudomonas sp. BIOMIG1 capable of degrading QACs in calcium alginate. Bioactive 3-mm alginate beads degraded benzalkonium chlorides (BACs), a group of QACs, with a rate of 0.47 mu M-BACs/h in shake flasks. A bench-scale continuous up-flow reactor packed with BIOMIG1-beads was operated over one and a half months with either synthetic wastewater or secondary effluent containing 2-20 mu M BACs at an empty bed contact time (EBCT) ranging between 0.6 and 4.7 h. Almost complete BAC removal was achieved from synthetic and real wastewater at and above 1.2 h EBCT without aeration and effluent recirculation. The microbial community in beads dominantly composed of BIOMIG1 with trace number of Achromobacter spp. after the operation of the reactor with the real wastewater, suggesting that BIOMIG1 over-competed native wastewater bacteria during the operation. This reactor system offers a low cost and robust treatment of QACs in wastewater. It can be integrated to conventional treatment systems for efficient removal of QACs from the wastewater, especially during the pandemic period.

Ergene River is heavily utilized for irrigation of fields to grow the main stocks of rice, wheat, and sunflower of Turkey also exported to Europe; therefore, monitoring the river's water quality is crucial for public health. Although the river quality is routinely monitored, the evaluation of pollution based on micropollutants is limited. In this study, we measured 222 organic micropollutants in 300 samples collected from 75 different locations on the Ergene River between August 2017 and May 2018 using direct injection liquid chromatography-tandem spectrometry with optimized scheduled multiple reaction monitoring. In total, 165 micropollutants were detected at a range of concentrations between 1.90 ng/L and 1824.55 μg/L. Sixty-three chemical substances were recurrent micropollutants that were detected at least one location in all seasons. Among them, 41 chemical substances were identified as the core micropollutants of the Ergene River using data-driven clustering methods. Hexa(methoxymethyl)melamine, benzotriazoles, and benzalkonium chlorides were frequently detected core micropollutants with an industrial origin. Besides, diuron, carbendazim, and cadusafos were common pesticides in the river. Core micropollutants were further categorized based on their type of source and environmental behavior using Kurtosis of concentration and load data obtained for each micropollutant. As a result, the majority of the core micropollutants are recalcitrant chemicals either released from a specific source located upstream of the river or have urban and agricultural sources dispersed on the watershed. In this study, we assessed the current state of pollution in the Ergene River at the micropollutant level with a very high spatial resolution and developed a statistical approach to categorize micropollutants that can be used to monitor the extent of pollution and track pollution sources in the river.

Pseudomonas sp. strain BIOMIG1BAC is an antibiotic-resistant gammaproteobacterium that can completely mineralize different homologs of benzalkonium chloride disinfectants. Here, we report the annotated complete genome sequence of this microorganism, which includes one circular chromosome with a length of 7,675,262 bp.

Acetaminophen (APAP), which is an active ingredient of many analgesic drugs, is one of the contaminants of emerging concern in the environment. Although APAP is biodegradable, it is frequently detected in treatment plant effluents, surface water and soil suggesting that there are factors affecting the fate of APAP in the environment. In this study, four strains of bacteria that can degrade APAP were isolated from soil. Those strains belonged to Rhodococcus, Pseudomonas, Flavobacterium, and Sphingobium genera of Bacteria. A series of kinetic experiments were performed on the isolates in shake-flasks to determine biodegradation rate constant as well as the effect of temperature, APAP concentration and cell density on the biodegradation rates. APAP biodegradation follows the first order reaction kinetics which is coupled with cell growth. The specific APAP biodegradation rate constant (k) for all strains was similar and equal to 0.19 +/- 0.01 h(-1). The temperature, at which APAP biodegradation rate was maximum, was 35 degrees C. APAP biodegradation rate was linearly correlated with both the initial APAP concentration and the cell density. Initial step of the APAP biodegradation was hydrolysis of the amide bond which resulted in formation and accumulation of p-aminophenol suggesting that aryl acylamidase enzyme is responsible for the biotransformation. In addition, free and immobilized crude enzymes of the isolates transformed APAP at similar rates, comparable to the intact cells. This study showed that APAP biodegradation is achieved by a diverse group of bacteria having a similar enzyme operating at a constant kinetics which is very slow at environmentally relevant APAP concentrations. Natural removal of APAP in the environment is limited by kinetics, therefore APAP-bearing waste streams should be treated in adsorption enhanced biological systems before discharged into the environment. (C) 2019 Elsevier B.V. All rights reserved.

The objective of this study is to develop an artificial neural network (ANN) based solution approach to predict the weekly flows of Ergene River which is the largest river in Thrace region of Turkey. In the developed approach, precipitation – flow data relationships have been investigated in order to establish the best model structure to predict streamflow at the selected basin. The developed relationships are then evaluated using a feed forward neural network where back propagation algorithm is used to determine the associated network weights. The performance of the developed ANN based solution approach is evaluated by using the weekly precipitation and flow data collected from different monitoring sites in Ergene River basin. The model results are also compared with HEC-HMS model outputs which is calibrated using the same precipitation and flow data. Results indicate that the proposed ANN based solution approach can be effectively used to predict the weekly flows of Ergene River.

While the misuse of antibiotics has clearly contributed to the emergence and proliferation of resistant bacterial pathogens, with major health consequences, it remains less clear if the widespread use of disinfectants, such as benzalkonium chlorides (BAC), a different class of biocides than antibiotics, has contributed to this problem. Here, we provide evidence that exposure to BAC coselects for antibiotic-resistant bacteria and describe the underlying genetic mechanisms. After inoculation with river sediment, BAC-fed bioreactors selected for several bacterial taxa, including the opportunistic pathogen Pseudomonas aeruginosa, that were more resistant to several antibiotics than their counterparts in a control (no BAC) bioreactor. A metagenomic analysis of the bioreactor microbial communities, confirmed by gene cloning experiments with the derived isolates, suggested that integrative and conjugative elements encoding a BAC efflux pump together with antibiotic resistance genes were responsible for these results. Furthermore, the exposure of the P. aeruginosa isolates to increasing concentrations of BAC selected for mutations in pmrB (polymyxin resistance) and physiological adaptations that contributed to a higher tolerance to polymyxin B and other antibiotics. The physiological adaptations included the overexpression of mexCD-oprJ multidrug efflux pump genes when BAC was added in the growth medium at subinhibitory concentrations. Collectively, our results demonstrated that disinfectants promote antibiotic resistance via several mechanisms and highlight the need to remediate (degrade) disinfectants in nontarget environments to further restrain the spread of antibiotic-resistant bacteria.IMPORTANCE Benzalkonium chlorides (BAC) are biocides broadly used in disinfectant solutions. Disinfectants are widely used in food processing lines, domestic households, and pharmaceutical products and are typically designed to have a different mode of action than antibiotics to avoid interfering with the use of the latter. Whether exposure to BAC makes bacteria more resistant to antibiotics remains an unresolved issue of obvious practical consequences for public health. Using an integrated approach that combines metagenomics of natural microbial communities with gene cloning experiments with isolates and experimental evolution assays, we show that the widely used benzalkonium chloride disinfectants promote clinically relevant antibiotic resistance. Therefore, more attention should be given to the usage of these disinfectants, and their fate in nontarget environments should be monitored more tightly.

A review of the literature published in 2013 on topics relating to the fate of emerging environmental pollutants during wastewater treatment is presented. This review is divided into the following sections: emerging biological agents, estrogens, metals, nanomaterials, nutrients, persistent organic pollutants, pharmaceuticals and personal care products and herbicide, and trace organic contaminants.

Aerobic biodegradation of naphthenic acids is of importance to the oil industry for the long-term management and environmental impact of process water and wastewater. The effect of structure, particularly the location of the alkyl side chain as well as cyclicity, on the aerobic biotransformation of 10 model naphthenic acids (NAs) was investigated. Using an aerobic, mixed culture, enriched with a commercial NA mixture (NA sodium salt; TCI Chemicals), batch biotransformation assays were conducted with individual model NAs, including eight 8-carbon isomers. It was shown that NAs with a quaternary carbon at the α- or β-position or a tertiary carbon at the β- and/or β'-position are recalcitrant or have limited biodegradability. In addition, branched NAs exhibited lag periods and lower degradation rates than nonbranched or simple cyclic NAs. Two NA isomers used in a closed bottle, aerobic biodegradation assay were mineralized, while 21 and 35% of the parent compound carbon was incorporated into the biomass. The NA biodegradation probability estimated by two widely used models (BIOWIN 2 and 6) and a recently developed model (OCHEM) was compared to the biodegradability of the 10 model NAs tested in this study as well as other related NAs. The biodegradation probability estimated by the OCHEM model agreed best with the experimental data and was best correlated with the measured NA biodegradation rate.

The biotransformation potential of a commercial naphthenic acid (NA) mixture (NA sodium salt; TCI Chemicals) under aerobic conditions was investigated using mixed aerobic cultures developed under various levels and duration of NA exposure. A culture enriched using the commercial NA mixture as the sole carbon source degraded NAs in a range of NA concentrations, regardless of culture age and the presence of a co-substrate; however, only 28.5% of the NA-carbon was detected as CO 2 while 44% was utilized for biomass growth. A fraction of the NA mixture (15–26%) was persistent under all conditions studied. In contrast, a culture fed with a degradable synthetic wastewater only (NA un-amended culture) and another culture fed with the same wastewater and 50 mg NA/L (NA-amended culture), over time lost their ability to degrade NAs. Analysis of the 16S rRNA gene based clone library revealed that 80% of the NA-enriched culture belonged to the γ-Proteobacteria class and was largely dominated by phylotypes most closely related to known NA and hydrocarbon degraders such as Pseudomonas and Microbulbifer . The results of this study indicate that although significant NA degradation is possible, only a small fraction of the NA mixture is completely mineralized to CO 2 . Further investigation into the biotransformation products and conditions affecting NA biodegradation under realistic refinery and environmental conditions will help to design effective treatment and bioremediation processes.

Naphthenic acids (NAs) are a complex group of alkyl-substituted acyclic, monocyclic and polycyclic carboxylic acids present in crude oil, oil sands process water and tailings ponds, as well as in refinery wastewater. Bioassays were performed to investigate the biotransformation potential and inhibitory effect of a commercial NA mixture to nitrification, denitrification and fermentation/methanogenesis using mixed cultures not previously exposed to NAs. NAs inhibited nitrification in a mixed aerobic heterotrophic/nitrifying culture at concentrations as low as 80 mg NA/L, whereas, an enriched nitrifying culture was only affected at 400 mg NA/L. The lower nitrification inhibition in the latter assay is attributed to the higher population size of nitrosofying and nitrifying bacteria compared to the mixed heterotrophic/nitrifying culture. The NA mixture was not inhibitory to denitrifiers up to 400 mg/L. At higher NA concentrations, cell lysis was pronounced and lysis products were the main source of degradable carbon driving denitrification in culture series prepared without an external carbon source. In the presence of a degradable external carbon source, no difference was observed in nitrate reduction rates or nitrogen gas production at all NA concentrations tested. Methanogenesis was completely inhibited at NA concentrations equal to or higher than 200 mg/L. Methanogenic culture series amended with 80 mg NA/L were transiently inhibited and methane production in culture series prepared with NAs and an external carbon source or NAs only recovered in 136 and 41 days, respectively. Accumulation of volatile fatty acids was observed at inhibitory NA concentrations; however, carbon dioxide production was not affected by NAs, indicating that fermentation and acidogenesis were not affected by NAs. NAs were not degraded under nitrate-reducing or fermentative/methanogenic conditions used in the present study, regardless of the presence or not of another, degradable carbon/energy source.

Recently, an array of eight genes involved in the biotransformation of benzalkonium chlorides (BACs)—an active ingredient of many disinfectants—to benzyldimethyl amine (BDMA) was identified in the genome of Pseudomonas sp. BIOMIG1, which is a bacterium present in various environments and mineralizes BACs. In this study, we showed that heterologous expression of an oxygenase gene (oxyBAC) present in this gene array in E. coli resulted in formation of BDMA from BACs at a rate of 14 μM h–1. oxyBAC is phylogenetically classified as a Rieske-type oxygenase (RO) and belongs to a group which catalyzes the cleavage of C–N+ bond between either methyl or alkyl ester and a quaternary nitrogen (N) of natural quaternary ammonium compounds such as stachydrine, carnitine, and trimethylglycine. Insertion of two glycines into the Rieske domain and substitution of tyrosine with leucine in the mononuclear iron center differentiate oxyBAC from other ROs that cleave C–N+, and presumably facilitate the cleavage of saturated al...

Ester quaternary ammonium compounds (esterquats), which are mainly used as active ingredients in fabric softeners and personal care products, are beginning to replace traditional quaternary ammonium compounds. As a result of hydrophobicity and increasing use, esterquats reach anaerobic treatment systems. However, little is known about the fate of esterquats under anaerobic conditions. In the present study, the potential inhibitory effect and biotransformation of two alkanoylcholines - acetylcholine chloride (ACh-Cl) and lauroylcholine chloride (LCh-Cl) - which are simple esterquats, under methanogenic conditions were investigated. ACh-Cl up to 300 mg/L was not inhibitory to a mixed methanogenic culture. In contrast, methanogenesis was inhibited by LCh-Cl above 50 mg/L, primarily caused by the accumulation of lauric acid which resulted from the abiotic hydrolysis of LCh. Below inhibitory concentrations, both ACh and LCh were transformed to methane by the mixed methanogenic culture. Mass spectrometric analysis confirmed that both alkanoylcholines were first abiotically hydrolyzed to choline and the corresponding alkanoic acid, which were then biotically transformed to methane, carbon dioxide, and ammonia. Thus, alkanoylcholine-containing waste streams can be bioprocessed to form methane, but hydrolysis products such as long-chain alkanoic acids may adversely impact the anaerobic bioconversion of alkanoylcholines.

The anaerobic biodegradability of municipal primary sludge, thickened waste activated sludge (TWAS), and fat, oil, and grease (FOG) was assessed using semi-continuous-feed, laboratory-scale anaerobic digesters and compared with the ultimate degradability obtained from 120-day batch digestion at 35 degrees C. In run 1, combined primary sludge and TWAS (40/60%, volatile solids [VS] basis) were fed to digesters operated at mesophilic (35 degrees C) and thermophilic (52 degrees C) temperatures at loading rates of 0.99 and 1.46 g-VS/L x d for primary sludge and TWAS, respectively, and a hydraulic retention time (HRT) of 12 days. The volatile solids destruction values were 25.3 and 30.7% (69 and 83% biodegradable volatile solids destruction) at 35 degrees C and 52 degrees C, respectively. The methane (CH4) yields were 159 and 197 mL at the standard temperature and pressure (STP) conditions of 0 degree C and 1 atm/g-VS added or 632 and 642 mL @ STP/g-VS destroyed at 35 degrees C and 52 degrees C, respectively. In run 2, a mix of primary sludge, TWAS, and FOG (21/31/48%, volatile solids basis) was fed to an acid digester operated at a 1-day HRT, at 35 degrees C, and a loading rate of 52.5 g-VS/L x d. The acid-reactor effluent was fed to two parallel methane-phase reactors operated at an HRT of 12 days and maintained at 35 degrees C and 52 degrees C, respectively. After an initial period of 20 days with near-zero gas production in the acid reactor, biogas production increased and stabilized to approximately 2 mL CH4 @ STP/g-VS added, corresponding to a volatile solids destruction of 0.4%. The acid-phase reactor achieved a 43% decrease in nonsaturated fat and a 16, 26, and 20% increase of soluble COD, volatile fatty acids, and ammonia, respectively. The methane-phase volatile solids destruction values in run 2 were 45 and 51% (85 and 97% biodegradable volatile solids destruction) at 35 degrees C and 52 degrees C, respectively. The methane yields for the methane-phase reactors were 473 and 551 mL @ STP/g-VS added, which is approximately 3 times larger compared with run 1, or 1040 and 1083 mL @ STP/g-VS destroyed, at 35 degrees C and 52 degrees C, respectively. The results indicate that, when co-digesting municipal sludge and FOG, a large FOG organic load fraction could have a profound effect on the methane gas yield.

The fate of six nitrosamines and their secondary amine precursors and total precursors in three municipal wastewater treatment plants' primary sludge (PS), waste-activated sludge (WAS), and anaerobic digester mixed liquor (ADML) was investigated. N-Nitrosodimethylamine was detected at significant concentrations, with mean concentrations at 678 +/- 302, 394 +/- 322, and 271 +/- 100 ng/L in PS, WAS, and ADML samples, respectively. N-Nitrosopyrrolidine was the other nitrosamine detected in sludge samples but at about an order of magnitude lower concentrations. PS samples also contained the highest concentrations of secondary amines(mostly dimethylamine (DMA) and pyrrolidine) followed by WAS and ADML samples, with mean DMA concentrations at 1280 +/- 689, 210 +/- 266, and 6.2 +/- 3.9 microg/L, respectively. Secondary amines in ADML and some WAS samples accounted for only 20-30% of total nitrosamine precursors underlining the significance of as of yet uncharacterized precursors. Overall, anaerobic sludge digestion was a sink for nitrosamines and secondary amines on the basis of the decreasing trends of these compounds from PS to WAS to ADML after taking mass balances into account An anaerobic bioassay conducted with ADML showed complete degradation of secondary amines even without additional carbon sources, while nitrosamine removal required carbon addition and was directly related to the chemical oxygen demand consumption.

The effect and transformation potential of benzalkonium chlorides (BAC) under nitrate reducing conditions were investigated at concentrations up to 100 mg/L in batch assays using a mixed, mesophilic (35 degrees C) methanogenic culture. Glucose was used as the carbon and energy source and the initial nitrate concentration was 70 mg N/L Dissimilatory nitrate reduction to ammonia (DNRA) and to dinitrogen (DNRN) were observed at BAC concentrations up to 25 mg/L At and above 50 mg BAC/L, DNRA was inhibited and DNRN was incomplete resulting in accumulation of nitrous oxide. Long-term inhibition of methanogenesis and accumulation of volatile fatty acids were observed at and above 50 mg BAC/L Over 99% of the added BAC was recovered from all cultures except the one amended with 100 mg BAC/L where 37% of the initially added BAC was transformed during the 100 day incubation period. Abiotic and biotic assays performed with 100 mg/L of BAC and 5 mM (in the liquid phase) of either nitrate, nitrite, or nitric oxide demonstrated that BAC transformation was abiotic and followed the modified Hofmann degradation pathway, i.e., bimolecular nucleophilic substitution with nitrite. Alkyl dimethyl amines (tertiary amines) were produced at equamolar levels to BAC transformed, but were not further degraded. This is the first report demonstrating the transformation of BAC under nitrate reducing conditions and elucidating the BAC transformation pathway.

Chromium(VI) reduction in the cathode of a microbial fuel cell (MFC) was investigated. An Htype MFC was operated with anaerobic anode and cathode compartments. The anode compartment was inoculated with an acetate-oxidizing mixed culture and the cathode compartment was inoculated with a mixture of denitrifying and anaerobic cultures enriched in the presence of Cr(VI). Acetate was provided to the anode compartment as substrate and bicarbonate was added to the cathode compartment as the sole inorganic carbon source. Fast Cr(VI) removal in the cathode compartment was observed along with acetate oxidation in the anode chamber and current generation. A maximum Cr(VI) reduction rate of 0.46 mg Cr(VI)/g VSS·h was achieved, which resulted in a current and power density of 123.4 mA/m 2 and 55.5 mW/m 2 , respectively. The reduced chromium was non-detectable in the supernatant of the catholyte, which indicates complete removal of chromium as Cr(OH)3 precipitate. The contribution of biomass decay and abiotic processes on Cr(VI) reduction was minimal, confirming that most of the Cr(VI) reduction was assisted by microbial activity in the cathode, which utilizes electrons and protons from the oxidation of acetate in the anode compartment. Analysis of the 16S rRNA gene based clone-library revealed that the cathode biomass was largely dominated by phylotypes closely related to Trichococcus pasteurii and Pseudomonas aeruginosa.

The anaerobic biodegradability of municipal primary and secondary sludge with increasing levels of partially dewatered fat, oil, and grease (FOG) was assessed using a mixed methanogenic culture at 35 "C. Under batch conditions with an acclimated and enriched microbial population, the sludge loading was 3 kg volatile solids/m3 and the highest FOG loading tested was 1.5 kg volatile solids/m3, resulting in a methane yield of 245 mL methane/g sludge volatile solids added at 35 degrees C and 1010 mL methane/g FOG volatile solids added at 35 degrees C. Under semicontinuous feeding conditions, the sludge and sludge plus FOG loading tested were 3 and 3.75 kg volatile solids/m3-d, respectively. Within 23 days of operation, the volatile fatty acid concentrations were reduced below 200 mg chemical oxygen demand/L (187 mg/L as acetic acid). Enhancement of sludge digestion was observed in those reactors where codigestion of sludge and FOG took place, which was attributed to a higher level of microbial activity maintained in these reactors as a result of FOG degradation. The results of this study demonstrate that beneficial use of FOG through codigestion with municipal sludge is feasible.

The effect of the quaternary ammonium compound, didecyl dimethyl ammonium chloride (DDAC), on nitrate reduction was investigated at concentrations up to 100 mg/L in a batch assay using a mixed, mesophilic (35°C) methanogenic culture. Glucose was used as the carbon and energy source and the initial nitrate concentration was 70 mg N/L. Dissimilatory nitrate reduction to ammonia (DNRA) and to dinitrogen (denitrification) were observed at DDAC concentrations up to 25 mg/L. At and above 50 mg DDAC/L, DNRA was inhibited and denitrification was incomplete resulting in accumulation of nitrous oxide. At DDAC concentrations above 10 mg/L, production of nitrous oxide, even transiently, resulted in complete, long-term inhibition of methanogenesis and accumulation of volatile fatty acids. Fermentation was inhibited at and above 75 mg DDAC/L. DDAC suppressed microbial growth and caused cell lysis at a concentration 50 mg/L or higher. Most of the added DDAC was adsorbed on the biomass. Over 96% of the added DDAC was recovered from all cultures at the end of the 100-days incubation period, indicating that DDAC did not degrade in the mixed methanogenic culture under the conditions of this study.

Quaternary ammonium compounds (QACs) and polyelectrolytes are extensively used in poultry processing facilities as sanitizing agents and flocculants, respectively. These chemicals may affect the performance of biological treatment systems resulting in low effluent quality. The impact of these chemicals on the anaerobic treatment of poultry processing wastewater (PPWW) samples, collected before and after a solids separation process, was tested in batch assays using a mixed, mesophilic (35 °C) methanogenic culture. The results of this study showed that Vigilquat ® (VQ), a commercial mixture of four QACs, has a high affinity for the organic solids in the PPWW. Cationic and anionic polyelectrolytes, alone or in combination, did not have any adverse effect on the anaerobic biodegradation of PPWW at concentrations typically used in poultry processing facilities (20 and 5 mg/L, respectively). In spite of the high affinity of VQ for the PPWW solids, VQ at a concentration of 50 mg/L and above adversely affected the anaerobic degradation of the PPWW, which resulted in a significantly reduced methane production and accumulation of volatile fatty acids. In the absence of any inhibition, the methane yield varied from 0.76 to 0.98 L methane at STP per g volatile solids added. VQ was not biodegraded under the batch, methanogenic conditions used in this study.

The scope of this study was to develop a continuous system to clean-up a trichloroethylene (TCE) contaminated gas stream, where biotic and abiotic removal mechanisms are undertaken sequentially simulating the horizontal bio- and chemo-barriers proposed for the in-situ remediation of the contaminated sites. The bio- and chemo-barriers were simulated by using glass columns packed with granular anaerobic mixed culture and Fe(0) filings, respectively. The effect of gas residence time, which is adjusted by the gas flowrate, on the TCE removal efficiency of the reactor system was investigated. TCE removal efficiency of over 90% was achieved at gas residence times above 1hr. Furthermore, the effluent of reactor system contained only ethane and ethylene, which are non-toxic by-products of TCE reduction reactions, along with trace amounts of TCE.

The combined effect of benzalkonium chloride (BAC) and prolonged exposure to low temperature on nitrification was investigated. Ammonia oxidation at 22-24°C by an enriched nitrifying culture was inhibited at increasing BAC concentrations and ceased at 15 mg BAC/L. The non-competitive inhibition coefficient was 1.5±0.9 mg BAC/L. Nitrification tests were conducted without and with BAC at 5mg/L using an aerobic, mixed heterotrophic/nitrifying culture maintained at a temperature range of 24-10°C. Maintaining this culture at 10°C for over one month in the absence of BAC, resulted in slower nitrification kinetics compared to those measured when the culture was first exposed to 10°C. BAC was degraded by the heterotrophic population, but its degradation rate decreased significantly as the culture temperature decreased to 10°C. These results confirm the negative impact of quaternary ammonium compounds on the nitrification process, which is further exacerbated by prolonged, low temperature conditions.

Disinfectants play an important role in maintaining acceptable health standards by significantly reducing microbial loads as well as reducing, if not eliminating, pathogens. This review focuses on quaternary ammonium compounds (QACs), a widely used class of organic disinfectants. Specifically, it reviews the occurrence, microbial adaptation, and degradation of QACs, focusing on recent reports on the ecology of QAC-degraders, the pathways and mechanisms of microbial adaptation which lead to resistance to QACs, as well as to antibiotics. With the help of culture-dependent and nonculture-dependent tools, as well as advanced analytical techniques, a better understanding of the fate and effect of QACs and their biotransformation products is emerging. Understanding the underlying mechanisms and conditions that result in QAC resistance and biodegradation will be instrumental in the prudent use of existing QAC formulations and foster the development of safer disinfectants. Development and implementation of (bio)technologies for the elimination of QACs from treated wastewater effluents will lessen adverse impacts to both humans and the environment.

Strategies for the transition of municipal sludge anaerobic digestion from mesophilic to thermophilic were assessed and the long-term stability and performance of thermophilic digesters operated at a solids retention time of 30 days were evaluated. Transition from 36 °C to 53.3 °C at a rate of 3 °C/day resulted in fluctuation of the daily gas and volatile fatty acids (VFAs) production. Steady-state was reached within 35 days from the onset of temperature increase. Transitions from either 36 or 53.3 °C to 60 °C resulted in relatively stable daily gas production, but VFAs remained at very high levels (in excess of 5000 mg COD/L) and methane production was lower than that of the mesophilic reactor. It was concluded that in order to achieve high VS and COD destruction and methane production, the temperature of continuous-flow, suspended growth digesters fed with mixed municipal sludge should be kept below 60 °C.

Anaerobic treatability and methane generation potential of cheese whey were determined in batch reactors. Furthermore, the effect of nutrient and trace metal supplementation on the batch anaerobic treatment, and the high-rate anaerobic treatability of cheese whey in upflow anaerobic sludge blanket (UASB) reactors were investigated. To this purpose biochemical methane potential experiments were conducted and single- and two-stage UASB reactors with granular cultures were operated. In UASB experiments significance of process staging, operational parameters such as hydraulic retention time (HRT), influent chemical oxygen demand (COD) concentration and loading rate were also investigated. The results revealed that nutrient and trace metal supplementation is vital for the anaerobic treatment of cheese whey; the anaerobic methane generation for the cheese whey studied was found to be 424 ml CH4/g COD (23.4 1 CH4/l cheese whey); undiluted cheese whey could be treated anaerobically at relatively short HRT values (2.06-4.95 days) without any significant stability problems; HRT values as low as 2-3 days can be used for the anaerobic treatment of cheese whey, with a COD removal efficiency of 95-97% at influent COD concentration of 42 700 +/- 141-55 100 +/- 283 mg/l.

The anaerobic treatability and methane generation potential of the wastewaters of the three important agro-industries in Turkey, namely, cheese-making, poultry breeding and the olive-oil mill industries were studied. Biochemical methane potential (BMP) experiments were conducted for different initial chemical oxygen demand (COD) concentrations. The results indicate that anaerobic treatment was possible for all the wastewaters studied and the biogas produced had a high methane content.

The effect of benzalkonium chlorides (BACs), a widely used class of quaternary ammonium disinfectants, on microbial community structure and antimicrobial resistance was investigated using three aerobic microbial communities: BACs-unexposed (DP, fed a mixture of dextrin/peptone), BACs-exposed (DPB, fed a mixture of dextrin/peptone and BACs), and BACs-enriched (B, fed only BACs). Long-term exposure to BACs reduced community diversity and resulted in the enrichment of BAC-resistant species, predominantly Pseudomonas species. Exposure of the two microbial communities to BACs significantly decreased their susceptibility to BACs as well as three clinically relevant antibiotics (penicillin G, tetracycline, ciprofloxacin). Increased resistance to BACs and penicillin G of the two BACs-exposed communities is predominantly attributed to degradation or transformation of these compounds, whereas resistance to tetracycline and ciprofloxacin is largely due to the activity of efflux pumps. Quantification of several key multidrug resistance genes showed a much higher number of copies of these genes in the DPB and B microbial communities compared to the DP community. Collectively, our findings indicate that exposure of a microbial community to BACs results in increased antibiotic resistance, which has important implications for both human and environmental health.

The inhibitory effect of two widely used quaternary ammonium compounds (QACs) – alkyl benzyl dimethyl (AB) and hexadecyl trimethyl (HD) ammonium chloride – on fermentation, methanogenesis and pentachloroaniline (PCA) dechlorination was assessed using a mixed, methanogenic, PCA-dechlorinating culture amended with AB or HD at a concentration range from 5 to 70 μM. PCA dechlorination was inhibited at 5 μM AB and was completely inhibited at 25 or 5 μM by AB or HD, respectively. However, the PCA dechlorination pathway was the same in both the QACs-free and QACs-amended culture series. Fermentation (acidogenesis) and methanogenesis were inhibited by both AB and HD at and above 25 μM but to a lesser degree than PCA dechlorination. Overall, HD resulted in a more severe inhibition of the mixed culture than AB. Adsorption of both QACs to the mixed culture biomass followed the Freundlich isotherm model. The adsorption affinity of HD for the mixed culture biomass was significantly higher than that of AB, which may be related to the observed higher inhibitory effects of HD compared to AB. Both AB and HD were not degraded in the mixed, dechlorinating culture used in this study.

The biotransformation of n-tetradecylbenzyldimethylammonium chloride (C(14)BDMA-Cl), a quaternary ammonium compound (QAC), under aerobic conditions by an enriched microbial community growing on benzalkonium chlorides (BACs) was investigated. Biotransformation of C(14)BDMA-Cl commenced with cleavage of the C(alkyl)-N bond and formation of benzyldimethylamine (BDMA). BDMA was further degraded, but in contrast to a previously reported BAC biotransformation pathway, neither benzylmethylamine (BMA) nor benzylamine (BA) was detected as a BDMA biotransformation product. Kinetic assays further confirmed that BMA and BA were not intermediates of C(14)BDMA-Cl transformation by the enriched community. Thus, BDMA is thought to be transformed to dimethylamine and benzoic acid via debenzylation. The biomass-normalized rate of C(14)BDMA-Cl biotransformation was 0.09 μmol/[mg of volatile suspended solids (VSS)·h]. The Microtox acute toxicity EC(50) value of BDMA was 500 times higher than that of C(14)BDMA-Cl. Thus, the aerobic biotransformation of C(14)BDMA-Cl to BDMA results in substantial toxicity reduction. Phylogenetic analysis of Bacteria diversity indicated that the majority of the sequenced clones (98% of the clone library) belonged to the genus Pseudomonas.