70 results on '"Ufuk, Özgen"'
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2. α-AMYLASE, α-GLUCOSIDASE AND LIPASE INHIBITORY PROPERTIES AND PHYTOCHEMICAL ANALYSIS OF ENDEMIC PLANT Jurinea brevicaulis Boiss.
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Nevin Ulaş Çolak, Ali Kandemir, Ufuk Özgen, Rezzan Aliyazıcıoğlu, Merve Badem, Şeyda Kanbolat, and Sıla Sener
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rp-hplc ,lipase ,jurinea ,antiobesity ,spme ,gc/ms ,Science (General) ,Q1-390 - Abstract
Obesity, defined as New World Syndrome, causes global health problems and big economic losses. Natural products have gained increasing importance because of their antiobesity potency. The genus Jurinea Cass. with approximately 200 described species worldwide has been traditionally used as a therapeutic agent for colic, fever, gout and rheumatism. The aim of this study was to analyze the volatile components, to determine phenolic compounds and to evaluate α-amylase, α-glucosidase, and lipase inhibitory activities of the endemic plant species Jurinea brevicaulis Boiss. The widely used solid-phase microextraction technique (SPME) was employed for Gas Chromatography-Mass Spectrometry (GC/MS) analysis of the volatile components. Quantitative analysis of phenolic compounds was performed using Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC). A total of 19 volatile components were specified and o-cymene (10.60 %), β-bisabolene (9.30 %), and sesquicineole (57.5 %) for different terpenes were described as major components. According to the RP-HPLC analysis, sinapic acid, p-coumaric acid and quercetin were determined for the species. IC50 values of the species were determined as 36.59 ± 2.37 μg/mL and 42.56 ± 2.83 μg/mL for α-amylase and α-glucosidase assays, respectively. IC50 value was found as 50.31 ± 3.75 μg/mL with the lipase inhibition analysis. In conclusion, it has been determined that J. brevicaulis included diverse volatile components, three phenolic compounds with antiobesity effect potential, which highlights J. brevicaulis as the up-and-coming candidate of natural product source to be used against obesity.
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- 2023
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3. Antidiabetic and Antioxidant Effect of the Aerial Parts of Lysimachia verticillaris and its Isolated Phenolic Compounds on Streptozotocin-induced Diabetic Rats
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Sıla Özlem ŞENER, Merve BADEM, Ufuk ÖZGEN, Nuriye KORKMAZ, Şeyda KANBOLAT, Gökçen KERİMOĞLU, Rezzan ALİYAZICIOĞLU, Engin YENİLMEZ, and Esen SEZEN KARAOĞLAN
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antidiabetic ,antioxidant ,apoptosis ,lysimachia verticillaris ,tunel ,Medicine (General) ,R5-920 - Abstract
Objective:Lysimachia genus has been recorded to be used in diabetes, traditionally and to have antidiabetic effect and antioxidant effects. The aim of this study was to evaluate the antidiabetic and antioxidant effects of aqueous extract of Lysimachia verticillaris (LV) and its isolated compounds, the percentage of apoptosis and histological changes in pancreatic β-cells in rats with streptozotocin (STZ)-induced type 1 diabetes mellitus.Methods:Male Spraque Dawley rats were divided into 6 groups. STZ (40 mg/kg) induced diabetic rats were treated orally with aqueous extract (400 mg/kg) and isolated compounds (20 mg/kg). To interpret antidiabetic effect, serum glucose and insulin levels were measured and morphological changes of pancreas were examined. Serum samples were analysed for catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPX) levels to investigate antioxidant effect. The terminal deoxynucleotidyl transferase (TDT)-mediated dUTP-biotin nick end-labeling (TUNEL) assays were performed to reveal apoptosis of pancreatic β-cells.Results:Oral administration of the extract and isolated compounds reduced high blood glucose levels (p
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- 2022
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4. Clinical Evidence-Based Pharmacokinetic Herbal Product/ Metabolite-Drug Interaction in the Treatment of Epilepsy: Traditional Review
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Tuğba SUBAŞ, İçim GÖKKAYA, Ufuk ÖZGEN, Gülin RENDA, and Sevim ŞAHİN
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- 2022
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5. Investigation of the Therapeutic Value of Verbascum pyramidatum Bieb. for Obesity
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Sıla SENER, Merve BADEM, Mehmet ÇATALBAŞ, Şeyda KANBOLAT, Ufuk ÖZGEN, and Nevin ULAŞ ÇOLAK
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Applied Mathematics - Abstract
Nowadays, one of the therapeutic approaches for obesity is the use pancreatic lipase inhibitors which reduce the digestion and absorption of fats. Most research indicates that natural sources which have a lipase inhibitory effect, may be utilized to treat obesity. Verbascum pyramidatum is one of the potential natural sources for obesity, and it has been demonstrated to have anti-inflammatory, antidiabetic, and regulatory effects on lipid metabolism. With this study, V. pyramidatum's potential lipase inhibitor effect, it is aimed to reveal its value in the treatment of obesity. In vitro spectroscopic method was used to determine the lipase inhibitory effect of V. pyramidatum. The quantitative investigation of V. pyramidatum's phenolic metabolites with antiobesity activity was carried out utilizing the Reverse Phase-High Performance Liquid Chromatography method. In this reported study, it was proven that extract and all fractions had an impact that inhibited lipase, with the ethyl acetate extract showing the highest inhibitory effect. Additionally, it was revealed through HPLC analysis that the species included p-OH benzoic acid, coumaric acid, quercetin, sinapic acid, and syringaldehyde. It has been demonstrated that V. pyramidatum may be a promising candidate for obesity treatment, but further investigations are required to use it as a therapeutic agent.
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- 2023
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6. Phytochemical Studies on Origanum rotundifolium
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Esen Sezen Karaoglan, Ufuk Özgen, and Cavit Kazaz
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Origanum rotundifolium ,Phytochemical study ,Therapeutics. Pharmacology ,RM1-950 - Published
- 2017
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7. Evaluation of biological activities of Barbarea integrifolia and isolation of a new glucosinolate derivated compound
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Rezzan Aliyazicioglu, Ihsan Calis, Emine Şalva, Seyda Kanbolat, Ali Kandemir, Nuriye Korkmaz, Merve Badem, Sermet Yildirmis, Ufuk Özgen, Sıla Ozlem Sener, and Kübra Kaban
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Barbarea ,Chromatography ,biology ,Chemistry ,DPPH ,Rosmarinic acid ,Ethyl acetate ,Phenolic acid ,biology.organism_classification ,General Biochemistry, Genetics and Molecular Biology ,Gluconasturtiin ,chemistry.chemical_compound ,Glucosinolate ,Kaempferol - Abstract
The aim of the present study is to determine the potent biological activities and carry out isolation studies on Barbarea integrifolia. The antioxidant capacity of the species was evaluated by total phenolic content, FRAP, CUPRAC, and DPPH radical scavenging activity. Anticancer activity studies were performed by MTT assay in MDA-MB-231, MCF-7, Hep3B, PC-3, A549, HCT116, L-929 cell lines. It was observed that the remaining aqueous fraction has higher total phenolic content while higher activity in the CUPRAC and FRAP assays was displayed for the methanolic extract and chloroform fraction. The extracts showed anticancer activity as compared with vincristine. It was observed that chloroform fraction has the highest anticancer activity on MCF-7 cell line, while ethyl acetate fraction has the highest anticancer activity on Hep-3B and A549 cell lines. Methanolic extract has the highest anticancer activity on HCT116 and MDA-MB-23 cell lines. The isolation studies have been performed using several chromatographic methods. The chemical structures of compounds have been identified by means of 1H NMR, 13C NMR, 2D-NMR, and MS. Five major compounds, one steroid (β-Sitosterol), one phenolic acid (Rosmarinic acid), one flavonol heteroside (kaempferol 7-O-α-l-rhamnoside-3-O-β-d-(2-O-β- d -glucosyl)-β-d-glucoside), and two glucosinolates (Gluconasturtiin, Gluconasturtiin choline salt) have been isolated.
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- 2021
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8. Isolation of Major Compounds and Gastroprotective Activity of Alchemilla Caucasica on Indomethacin Induced Gastric Ulcers in Rats
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Abdulmecit Albayrak, Aysenur Kahramanlar, Ufuk Özgen, Yasin Bayir, Cavit Kazaz, Esen Sezen Karaoglan, Elif Cadirci, and Erdem Toktay
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Antioxidant ,medicine.medical_treatment ,Ulcer index ,01 natural sciences ,Superoxide dismutase ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,medicine ,lcsh:R5-920 ,biology ,Traditional medicine ,business.industry ,Catechin ,General Medicine ,biology.organism_classification ,Malondialdehyde ,0104 chemical sciences ,010404 medicinal & biomolecular chemistry ,chemistry ,Phytochemical ,030220 oncology & carcinogenesis ,Apigenin ,biology.protein ,lcsh:Medicine (General) ,business ,Alchemilla - Abstract
Objective The Alchemilla genus, which belongs to the Rosaceae family, is known as Lady's mantle and is commonly used in traditional medicine. This study was designed to investigate the major metabolites isolation and gastroprotective effects of Alchemilla caucasica. Materials and methods Phytochemical studies were carried out using column chromatography on Alchemilla caucasica. The gastroprotective effect of ethanol extract of this plant was tested on indomethacin-induced gastric ulcer model in rats. In addition, superoxide dismutase (SOD), malondialdehyde (MDA), and glutathione (GSH) parameters in the stomach tissue were examined. Results Quercetin-3-O-glucuronide, apigenin, and catechin were isolated from aerial parts of Alchemilla caucasica. When macroscopic ulcer index and histopathological results were analyzed, the extract at 200 mg/kg dose was found to be most effective. All doses of extract reduced MDA level and enhanced SOD activity and GSH level. Conclusion The results of this study showed that Alchemilla caucasica has significant antiulcer activity. This effect was thought to be caused by antioxidant properties of flavonoids.
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- 2020
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9. Cholinesterase and Tyrosinase Inhibitory Potential and Antioxidant Capacity of <İ>Lysimachia verticillaris</İ> L. and Isolation of the Major Compounds
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Ahmet C. Gören, Sıla Ozlem Sener, Karel Šmejkal, Fatma Sezer Senol Deniz, Emil Švajdlenka, İlkay Erdoğan Orhan, Jiri Vaclavik, Ufuk Özgen, and Milan Žemlička
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chemistry.chemical_classification ,Antioxidant ,Chromatography ,medicine.medical_treatment ,Tyrosinase ,Flavonoid ,Pharmaceutical Science ,Biological activity ,chemistry.chemical_compound ,chemistry ,medicine ,Molecular Medicine ,Myricetin ,Gallic acid ,Quercetin ,Butyrylcholinesterase - Abstract
Objectives The scope of the present study was to specify the therapeutic potential for neurodegenerative diseases through evaluating cholinesterase and tyrosinase (TYR) inhibitory and antioxidant activity of Lysimachia verticillaris (LV), and to isolate the major compounds considering the most active fraction. Materials and methods The methanol extract (ME) of LV and the chloroform, ethyl acetate (EtOAC), and aqueous fractions obtained from it were used for biological activity and isolation studies. The ME and all fractions were tested for their acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and TYR inhibitory and antioxidant potentials using ELISA microtiter assays. Seven major compounds were isolated from the active EtOAC fraction by semi-preparative high performance liquid chromatography. The structures of the compounds were elucidated by several spectroscopic methods. Results Marked AChE inhibitory activity was observed in the EtOAC fraction (6337±1.74%), BChE inhibitory activity in the ME and EtOAC fraction (85.84±3.01% and 83.82±3.93%), total phenol content in the EtOAC fraction (261.59±3.95 mg equivalent of gallic acid/1 g of extract) and total flavonoid contents in the EtOAC fraction (515.54±2.80 mg equivalent of quercetin/1 g of extract), and 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and ferric-reducing antioxidant power values in the aqueous and EtOAC fractions (92.54±0.67%, 92.11±0.30%; 2.318±0.054, 2.224±0.091, respectively). Accordingly, the isolation studies were carried out on the EtOAC fractions. Compounds 1-7 (gallic acid, (+)-catechin, myricetin 3-O-arabinofuranoside, myricetin 3-O-α-rhamnopyranoside, quercetin 3-O-β-glucopyranoside, quercetin 3-O-arabinofuranoside, and quercetin 3-O-α-rhamnopyranoside, respectively) were isolated from the active EtOAC fraction. Conclusion LV may be a potential herbal source for treatment of neurodegenerative diseases based on its strong antioxidant activity and significant cholinesterase inhibition similar to that of the reference.
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- 2020
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10. Evaluation of LC-MS/MS Analysis and In Vitro Biological Activities of Rosa pimpinellifolia Root, Pseudo-fruit, and Seed extracts
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Leyla GÜVEN, Ufuk ÖZGEN, Handan SEVİNDİK, İclal AĞAN, Mehmet KOCA, İbrahim TURAN, Selim DEMİR, and Yüksel ALİYAZICIOĞLU
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Biological Activities ,LC/MS/MS ,Rosa pimpinellifolia ,Rosaceae ,Biochemistry and Molecular Biology ,Biyokimya ve Moleküler Biyoloji ,General Medicine - Abstract
In this study, we investigated antioxidant, anticholinesterase, antityrosinase properties, and polyphenolic and flavonoid content by spectrophotometric; antimicrobial by disc diffusion and microdilution method; antigenotoxic activities by comet assay, and the phytochemical composition of the Rosa pimpinellifolia extracts by LC-MS/MS. The EtOAc extract of the root (RPKE) had higher antioxidant activities at 10 μg/mL with inhibition of 39.7, 91.2, and 39.5% respectively in the DPPH·, ABTS•+, and superoxide anion radical scavenging activity assay than standard antioxidant molecules. The polyphenolic contents of the RPKE and the EtOAc extract of the seed (RPÇE) were found to be 378.2 ± 0.477 and 305.39 ± 0.568 μg GAE/mg respectively. The extracts of RPKE showed butyrylcholinesterase activity with 19±0.12% inhibition at 100 μg/mL concentration. RPKE showed higher activity at 500 μg/mL with inhibition of 50% in the tyrosinase inhibitory assay than the other R. pimpinellifolia extracts. The extracts of R. pimpinellifolia exhibited antimicrobial activity against Staphylococcus aureus and Candida albicans. The extracts of R. pimpinellifolia did not show any antigenotoxic effect up to the concentration of 1000 μg/mL. In LC/MS/MS analysis, Cyanidin-3-O-Glucoside and isoquercetin in the pseudo-fruit; procyanidin B2 and catechin in the root were the major phenolic compounds.
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- 2022
11. Paeoniflorigenone purified from Paeonia daurica roots potently inhibits viral and bacterial DNA polymerases: investigation by experimental validation and docking simulation
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Cemal Sandalli, Mustafa Emirik, Emine Akyüz Turumtay, Halbay Turumtay, Seyda Kanbolat, Adem Demir, and Ufuk Özgen
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ABTS ,biology ,010405 organic chemistry ,DPPH ,DNA polymerase ,Organic Chemistry ,Paeoniflorin ,Ascorbic acid ,biology.organism_classification ,01 natural sciences ,0104 chemical sciences ,010404 medicinal & biomolecular chemistry ,chemistry.chemical_compound ,chemistry ,Biochemistry ,biology.protein ,Paeonia daurica ,General Pharmacology, Toxicology and Pharmaceutics ,Polymerase ,DNA - Abstract
The methanolic extracts from fruit, leaf, stem and roots of Paeonia daurica subsp. macrophylla (P. daurica) were investigated for inhibitory effect on replicative bacterial (PolC and DnaE2) and viral (MMLV-RT from Moloney Murine Leukemia Virus) DNA polymerases by primer extension assay. While all plant parts showed inhibition effect on bacterial and viral DNA polymerases, roots of the plant was focused to purify inhibitory compound(s). The chemical structures of compounds were completely elucidated using a combination of NMR, MS and FT-IR analyses. Five molecules with tree monoterpene glycosides, paeoniflorin (PD-2), paeoniflorigenone (PD-4), benzoyl paeoniflorin (PD-5), and benzoic acid (PD-3) with its derivate 2,4,6-trihydroxy-1-methyl benzoate (PD-1) were purified and identified. Both DNAdependent and RNA-dependent polymerase activity of MMLV-RT was strongly inhibited by these five molecules. On the other hand, bacterial polymerases PolC and DnaE2 were strongly inhibited by only paeoniflorigenone (PD-4). Molecular modeling result suggested that paeoniflorigenone (PD-4) interacts with the important residues at active site (palm, fingers and thumb domains) of three polymerases which support our experimental result. Ethyl acetate fraction had smallest SC50 value against DPPH and ABTS radicals. It showed also higher scavenging activity than quercetin, trolox and ascorbic acid since its quite high total phenolic content. We proposed that the parts of P. daurica might be used to find new antimicrobial agents and generate supplementary material for foods. Furthermore, the isolated molecules with inhibitory effect may be used as new scaffold for the further modification in order to develop novel inhibitors against DNA polymerization.
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- 2019
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12. Dorycnium pentaphyllumExtract Has Antiproliferative Effect on Human Cervix and Colon Cancer Cells
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Selim Demir, Rezzan Aliyazicioglu, Orhan Deger, Sıla Ozlem Sener, Yuksel Aliyazicioglu, Elif Ayazoglu Demir, Ufuk Özgen, Ahmet Mentese, and Serap Ozer Yaman
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0301 basic medicine ,Cancer Research ,030109 nutrition & dietetics ,Nutrition and Dietetics ,Traditional medicine ,biology ,Colorectal cancer ,Medicine (miscellaneous) ,macromolecular substances ,Gallic acid metabolism ,NUTRITION&DIETETICS ,medicine.disease ,biology.organism_classification ,03 medical and health sciences ,0302 clinical medicine ,medicine.anatomical_structure ,Oncology ,Dorycnium pentaphyllum ,030220 oncology & carcinogenesis ,medicine ,Cytotoxic T cell ,Antiproliferative effect ,Cervix - Abstract
Although several studies have investigated the cytotoxic effects of different Fabaceae species, limited researches have been conducted on the cytotoxic effect of Dorycnium pentaphyllum. The aim of ...
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- 2019
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13. Investigation of antioxidant, cytotoxic, tyrosinase inhibitory activities, and phenolic profiles of green, white, and black teas
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Rezzan Aliyazicioglu, Mahmoud Abudayyak, Ezgi Öztaş, Nuriye Korkmaz, Seyda Akkaya, Merve Badem, Sıla Ozlem Sener, and Ufuk Özgen
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0303 health sciences ,Antioxidant ,Traditional medicine ,030306 microbiology ,Chemistry ,medicine.medical_treatment ,Tyrosinase ,Biochemistry (medical) ,Clinical Biochemistry ,04 agricultural and veterinary sciences ,Inhibitory postsynaptic potential ,040401 food science ,Biochemistry ,White (mutation) ,03 medical and health sciences ,0404 agricultural biotechnology ,medicine ,Cytotoxic T cell ,Molecular Biology ,Black tea - Abstract
Objectives To determine of antioxidant, cytotoxic and tyrosinase inhibitory (TI) activities, and phenolic profiles with a new high performance liquid chromatography (HPLC) method of green, white and black teas. Methods Antioxidant activities of the teas were examined by means of scavenging of the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), and cupric reducing antioxidant capacity (CUPRAC). The phenolic contents were investigated by means of HPLC. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to evaluate the cytotoxic potential of tea extracts in cancer and normal cell lines. TI activity was investigated against diphenolase (DOPA), using kojic acid as a positive control. Results The green tea extract exhibited stronger antioxidant than white and black tea extracts. The green tea contained syringaldehyde, p-coumaric acid, benzoic acid, and quercetin. The white tea extract had the highest tyrosinase inhibitor activity. The extracts exhibited higher cytotoxic potential toward cancer cells than normal cells. The methanol extract of green tea had the highest cytotoxic potential, while the water extracts were less toxic. Conclusion The green, white, and black teas can be regarded as an important in terms of rich antioxidant, tyrosinase inhibitor, and cytotoxic activities in both the pharmaceutical and food industries.
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- 2019
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14. Evaluation of Antioxidant, Antimicrobial, Antityrosinase and Cytotoxic Potentials of Isatis cappadocica subsp. alyssifoli as a Potent Pharmaceutical Resource
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Meltem Güner, Şengül Alpay Karaoğlu, Nuriye Korkmaz, Mahmoud Abudayyak, Ali Kandemir, Seyda Kanbolat, Ufuk Özgen, Atila T. Kalaycioglu, Rezzan Aliyazicioglu, RTEÜ, Fen - Edebiyat Fakültesi, Biyoloji Bölümü, and Karaoğlu, Şengül Alpay
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Antioxidant ,Isatis cappadocica ,Traditional medicine ,biology ,Chemistry ,DPPH ,Antityrosinase ,Cytotoxicity ,medicine.medical_treatment ,Tyrosinase ,Isatis ,biology.organism_classification ,Antimicrobial ,High-performance liquid chromatography ,chemistry.chemical_compound ,medicine ,Bacteria ,Benzoic acid - Abstract
WOS: 000464004500002 Isatis species, which are endemic across most of Turkey, exhibit antibacterial, anticancer and antiviral effects. the aim of this study was to determine of antioxidant, antimicrobial, tyrosinase inhibitor, cytotoxic activities, and phenolic profile of polar extracts of Isatis cappadocica Desv. We analyzed the antioxidant properties of extracts using total phenolic content (TPC), ferric reducing antioxidant power (FRAP), cupric reducing antioxidant capacity (CUPRAC), and 2,2-diphenylpicrylhydrazyl (DPPH) radical scavenging activity. the phenolic composition of methanolic extract of I. cappadocica was analyzed by reverse phase high performance liquid chromatography (RP-HPLC). Extracts' antimicrobial properties were evaluated based on the agar well diffusion technique. Tyrosinase inhibitory activity was measured colorimetrically. Methanolic extract yielded better FRAP and CUPRAC results and aqueous extract yielded better DPPH activity. Benzoic acid, and sinapic acid were detected as major phenolic compounds. Methanolic extract was particularly effective against all the bacteria investigated, apart from Yersinia pseudotuberculosis. Methanol extract was exhibited tyrosinase inhibitory activity. the methanol extract has caused to death of cells by dosage in the high concentrations cytotoxic activity on the PC-3 and 3T3 cell lines. the results showed that I. cappadocica could be used as a natural source in the food, cosmetic, and drug industries due to their rich antioxidant, antimicrobial, cytotoxic and tyrosinase inhibitor activities.
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- 2019
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15. Phosphodiesterase-1 inhibitory potential of several natural products by molecular docking approach
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Ufuk Özgen, Erhan Palaska, Abdur Rauf, Ilkay Erdogan Orhan, Muhammad Saleem, Esen Sezen Karaoglan, Gülin Renda, and Muslim Raza
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chemistry.chemical_classification ,Inhibitory potential ,Stereochemistry ,Phosphodiesterase ,Plant Science ,Biochemistry ,chemistry.chemical_compound ,Enzyme ,chemistry ,Smooth muscle ,Docking (molecular) ,Soyasaponin II ,Apigenin ,Agronomy and Crop Science ,Luteolin ,Biotechnology - Abstract
Natural products of herbal origin are well-known to exhibit diverse biological activities. In the present study, a number of natural compounds of plant origin; luteolin 5-O-beta-glucoside (1), methyl rosmarinate (2), apigenin (3), vicenin 2 (4), lithospermic acid (5), soyasaponin II (6), and rubiadin 3-O-beta-primeveroside (7) were tested at 0.5mM using ELISA microtiter assay against phosphodiesterase-1 (PDE-1), which plays role in smooth muscle activity and inflammation. Among the tested compounds, luteolin 5-O-beta-glucoside (1) (IC50 = 125.01 +/- 1.24 mu M), apigenin (3) (IC50 = 62.50 +/- 1.56 mu M), and rubiadin 3-O-beta-primeveroside (7) (IC50 = 93.75 +/- 2.50 mu M) displayed a strong inhibition as compared to the reference (EDTA, IC50 = 270.11 +/- 2.65 mu M). Besides, the active three compounds (1, 3, and 7) were proceeded to molecular docking experiments using two docking softwares (Autodock Vina and i-GEMDOCK) and showed clear interactions with active gorge of the enzyme through hydrogen bondings as well as hydrophobic contacts.
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- 2019
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16. Outlining In Vitro and In Silico Cholinesterase Inhibitory Activity of Twenty-Four Natural Products of Various Chemical Classes: Smilagenin, Kokusaginine, and Methyl Rosmarinate as Emboldening Inhibitors
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Ihsan Calis, F. Sezer Senol Deniz, Bilge Sener, Randa Aldaba, Gokcen Eren, Ufuk Özgen, and Ilkay Erdogan Orhan
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smilagenin ,natural products ,Molecular Conformation ,Pharmaceutical Science ,Pharmacology ,01 natural sciences ,Analytical Chemistry ,chemistry.chemical_compound ,0302 clinical medicine ,Verbascoside ,Drug Discovery ,Butyrylcholinesterase ,biology ,Molecular Structure ,Chemistry ,Diosgenin ,cholinesterase inhibition ,Acetylcholinesterase ,Molecular Docking Simulation ,Chemistry (miscellaneous) ,language ,Quinolines ,Molecular Medicine ,methyl rosmarinate ,Alzheimer’s disease ,Protein Binding ,Aché ,Molecular Dynamics Simulation ,Article ,lcsh:QD241-441 ,03 medical and health sciences ,Inhibitory Concentration 50 ,Structure-Activity Relationship ,lcsh:Organic chemistry ,Spirostans ,Physical and Theoretical Chemistry ,Furans ,IC50 ,Cholinesterase ,Biological Products ,Binding Sites ,Organic Chemistry ,molecular docking ,In vitro ,language.human_language ,kokusaginine ,0104 chemical sciences ,010404 medicinal & biomolecular chemistry ,biology.protein ,Cholinesterase Inhibitors ,030217 neurology & neurosurgery - Abstract
Cholinesterase (ChE) inhibition is an important treatment strategy for Alzheimer’s disease (AD) as acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) are involved in the pathology of AD. In the current work, ChE inhibitory potential of twenty-four natural products from different chemical classes (i.e., diosgenin, hecogenin, rockogenin, smilagenin, tigogenin, astrasieversianins II and X, astragalosides I, IV, and VI, cyclocanthosides E and G, macrophyllosaponins A-D, kokusaginin, lamiide, forsythoside B, verbascoside, alyssonoside, ipolamide, methyl rosmarinate, and luteolin-7-O-glucuronide) was examined using ELISA microtiter assay. Among them, only smilagenin and kokusaginine displayed inhibitory action against AChE (IC50 = 43.29 ± 1.38 and 70.24 ± 2.87 µg/mL, respectively). BChE was inhibited by only methyl rosmarinate and kokusaginine (IC50 = 41.46 ± 2.83 and 61.40 ± 3.67 µg/mL, respectively). IC50 values for galantamine as the reference drug were 1.33 ± 0.11 µg/mL for AChE and 52.31 ± 3.04 µg/mL for BChE. Molecular docking experiments showed that the orientation of smilagenin and kokusaginine was mainly driven by the interactions with the peripheral anionic site (PAS) comprising residues of hAChE, while kokusaginine and methyl rosmarinate were able to access deeper into the active gorge in hBChE. Our data indicate that similagenin, kokusaginine, and methyl rosmarinate could be hit compounds for designing novel anti-Alzheimer agents.
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- 2021
17. Investigation Of Therapeutic Potential Of Three Endemic Cirsium Species For Global Health Problem Obesity
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Tuncay Dirmenci, Gülçin Saltan İşcan, Nuriye Korkmaz, Hatice Hancı, Merve Badem, Turan Arabaci, Sıla Ozlem Sener, Engin Yenilmez, Ufuk Özgen, Seyda Kanbolat, and Rezzan Aliyazicioglu
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0106 biological sciences ,Triglyceride ,Adiponectin ,Plant Science ,Biology ,Pharmacology ,01 natural sciences ,0104 chemical sciences ,010404 medicinal & biomolecular chemistry ,chemistry.chemical_compound ,Orlistat ,Fatty acid synthase ,chemistry ,medicine ,biology.protein ,Astragalin ,Afzelin ,010606 plant biology & botany ,Lupeol ,Lipoprotein ,medicine.drug - Abstract
Obesity is a risk factor for metabolic disorders causes serious health problems and economic costs globally. Some Cirsium genus has been reported to possess anti-obesity effect and traditional uses for vascular diseases, anti-inflammatory and diuretic effects. This study aims to investigate the therapeutic value of endemic Cirsium species for obesity treatment. C. balikesirense Yildiz, Arabaci & Dirmenci (CB), C. nerimaniae Yildiz, Dirmenci & Arabaci (CN) and C. sommieri Petr. (CS) were selected for the study. High-fat diet (HFD) induced obese Sprague Dawley rats were employed for in vivo studies. The extract obtained aerial parts of Cirsium species were applied to rats orally for 8 weeks. To interpret the antiobesity activity, body weight of the rats were measured. Also, biochemical, histopathological and quantitative real-time PCR (RT-PCR) analysis were performed on rats. Bioactivity-guided fractionation studies were carried out guidance with in vitro lipase enzyme inhibition. The structures of the pure compounds purified using various chromatographic techniques were determined by spectroscopic methods. Oral administration of the extracts and orlistat decreased in body weights, adipocytes size, levels of serum low-density lipoprotein (LDL), triglyceride (TG), leptin levels, lipase, tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), and increased levels of serum high-density lipoprotein (HDL), adiponectin. According to RT-PCR analysis, the extracts reduced the levels of key transcription factors in adipogenesis and fatty acid synthase (FAS). Bioactivity-guided fractionation studies led to isolation of active compounds as syringin, cimidahurinin, astragalin, afzelin, lupeol, and psi-taraxasterol. In conclusion, CB, CN, CS and their isolated compounds were uncovered to be hopeful as a candidate for antiobesity therapeutics. (C) 2021 SAAB. Published by Elsevier B.V. All rights reserved.
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- 2021
18. Composition and bioactivity of essential oil from Stachys macrostachya (Wend.) Briq
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Bilal Yilmaz, Arzu Görmez, Ufuk Özgen, Fatma Necmiye Kaci, and Esen Sezen Karaoglan
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Acinetobacter baumannii ,Tyrosinase ,Science ,Microbial Sensitivity Tests ,essential oil ,Gas Chromatography-Mass Spectrometry ,law.invention ,chemistry.chemical_compound ,Valencene ,law ,Oils, Volatile ,Essential oil ,Multidisciplinary ,Lamiaceae ,biology ,Traditional medicine ,GC/MS ,Stachys macrostachya ,Stachys ,biology.organism_classification ,Antimicrobial ,Anti-Bacterial Agents ,tyrosinase inhibitory activity ,chemistry ,Gas chromatography ,Antibacterial activity - Abstract
Stachys species belonging to Lamiaceae family have been used for medicinal purposes since ancient times. The aim of the present study was to investigate the chemical compositions and antibacterial, anti-tyrosinase activities of the essential oil of Stachys macrostachya. The essential oil was prepared by hydrodistillation method using a Clevenger-type apparatus and chemical composition was determined by gas chromatography (GC). The antibacterial activity of essential oil was performed by the disc diffusion and microdilution broth method against five Gram-positive and two Gram-negative bacteria. The tyrosinase inhibitory activity was evaluated by minor modifications of Masuda's method. According to the results of GC analyses, twenty-three compounds were identified representing 91.9% of the total volatile composition. The main compounds were germacrene D (12.2%), globulol (10.9%), alpha-pinene (9.7%), and valencene (7.6%). The present study showed that the tested essential oil of S. macrostachya exhibited antibacterial activity against Acinetobacter baumannii (MIC 62.50 mu g/mL) and tyrosinase inhibition activity (IC50 22.86 +/- 0.82 mu g/mL). These results suggest that the essential oil could be exploited as a potential source of natural antimicrobial agents of this bacterium as well as tyrosinase inhibitors.
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- 2020
19. Evaluation of Cytotoxic Effect of Onosma armeniacum Extract on Various Cancer Cells
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Ibrahim Turan, Selim Demir, Elif Ayazoglu Demir, and Ufuk Özgen
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biology ,Traditional medicine ,Basic Sciences ,Urology ,Temel Bilimler ,Onosma ,biology.organism_classification ,Nephrology ,Antioxidant activity,Cancer,Cell culture,Cytotoxicity,Onosma armeniacum ,Cancer cell ,Cytotoxic T cell ,Biology ,Antioksidan aktivite,Hücre kültürü,Kanser,Onosma armeniacum,Sitotoksisite ,Biyoloji - Abstract
Bu çalışmanın amacı O. armeniacum kök ekstraktının antioksidan özellikleri ile normal bir fibroblast hücre hattına kıyasla insan karaciğer (HepG2), akciğer (A549) ve kolon (WiDr) kanser hücre hatlarındaki sitotoksik etkisini araştırmaktır. Ekstraktın antioksidan özellikleri kolorimetrik yöntemler kullanılarak belirlenmiştir. Ekstraktın sitotoksik etkisi MTT yöntemi kullanılarak araştırılmıştır. Ekstraktın toplam fenolik içerik ve indirgeyici güç değerleri, g örnek başına sırasıyla 26.1±0.4 mg gallik asit eşdeğeri ve 52.6±1.1 mg troloks eşdeğeri olarak hesaplanmıştır. Ekstrakt, çalışılan tüm kanser hücre serilerinde, özellikle HepG2 (3.6 kat), seçici sitotoksik etki göstermiştir. Çalışmanın sonuçları, kabul edilebilir antioksidan ve sitotoksik etkilere sahip olduğu saptanan O. armeniacum'un daha kapsamlı çalışmalarla incelenmesi gerektiğini ortaya koymaktadır., The aim of this study was to investigate the antioxidant properties of the root extract of Onosma armeniacum and its cytotoxic effect against human liver (HepG2), lung (A549) and colon (WiDr) cancer cell lines compared with a normal fibroblast cell line. Antioxidant properties of the extract was determined using the colorimetric methods. The cytotoxic effect of the extract were investigated using MTT assay. The total phenolic content and reducing power values of extract were 26.1±0.4 mg gallic acid equivalent and 52.6±1.1 mg trolox equivalent per g sample, respectively. The extract exhibited selective cytotoxic effect in all studied cancer cell lines, especially HepG2 (3.6-fold). The results of this study reveal that O. armeniacum, which has been found to have acceptable antioxidant and cytotoxic effects, should be examined with more comprehensive studies.
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- 2020
20. Antioxidant, Antimicrobial, Cytotoxic, Anticholinesterase, Antityrosinase Activities and Characterisation of Volatile Compounds of Verbascum oocarpum by SPME and GC-FID/MS
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Rezzan Aliyazicioglu, Mahmoud Abudayyak, Şengül Alpay Karaoğlu, Merve Badem, Seyda Kanbolat, Nevin Ulas Colak, Nuriye Korkmaz, Ali Kandemir, Ufuk Özgen, Sıla Ozlem Sener, EBYÜ, Fen Edebiyat Fakültesi, RTEÜ, Fen - Edebiyat Fakültesi, Biyoloji Bölümü, and Karaoğlu, Şengül Alpay
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0106 biological sciences ,Antioxidant ,Traditional medicine ,biology ,Chemistry ,medicine.medical_treatment ,Pharmacology toxicology ,Volatile components ,Verbascum oocarpum ,Antimicrobial ,biology.organism_classification ,01 natural sciences ,Gc fid ms ,03 medical and health sciences ,0302 clinical medicine ,volatile components ,Verbascum ,medicine ,antimicrobial ,Cytotoxic T cell ,030217 neurology & neurosurgery ,010606 plant biology & botany - Abstract
WOS: 000454630200005 The objective of present study was to specify the antioxidant, antimicrobial, cytotoxic, anticholinesterase, and antityrosinase activities of Verbascum oocarpum Murb., together with its volatile components. the methanolic and aqueous extracts of aerial parts of V. oocarpum indicated substantial antioxidant activity derived from benzoic acid, sinapic acid, and quercetin compounds. the methanolic extract displayed moderate antimicrobial activity against Escherichia coli, Pseudomonas aeruginosa, and Mycobacterium smegmatis. It also exhibited a higher IC50 value on tyrosinase than kojic acid, and lower acetylcholinesterase and butyrylcholinesterase inhibitor activities than galantamin. MTT analysis revealed that high concentrations of V. oocarpum extract can result in cytotoxicity, with an IC50 of 0.444 mg/mL. Common volatile components included pentadecane, hex-2(E)-enal, limonene, phenylacetaldehyde, isophorone, 1-methoxy-4-(2-propenyl)-benzene, 5,6,7,7a-tetrahydro-4,4,7a-trimethyl-2(4h)-benzofuranone and hexadecane. V. oocarpummay be of potential benefit to the food, cosmetical, and pharmaceutical sectors owing to its potent anticholinesterase, antioxidant, and antimicrobial activities, volatile components, and limited cytotoxicity. Turkish Scientific and Technical Research Council (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) Sila Ozlem Sener, Merve Badem and Nuriye Korkmaz would like to acknowledge the scholarship during their postgraduate program provided by the Turkish Scientific and Technical Research Council (TUBITAK).
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- 2018
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21. Phytochemical studies on the seeds, pseudofruits, and roots of Rosa pimpinellifolia
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GÜVEN-Ufuk ÖZGEN-Hasan SEÇEN, Leyla, primary
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- 2021
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22. Flavonoid Derivatives from the Aerial Parts of Trifolium trichocephalum M. Bieb. and Their Antioxidant and Cytotoxic Activity
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Ilkay Erdogan Orhan, Zeynep Ünal, Gülin Renda, Ufuk Özgen, Erhan Palaska, and Suna Sabuncuoğlu
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Antioxidant ,medicine.medical_treatment ,Trifolium trichocephalum ,Plant Science ,01 natural sciences ,lcsh:Chemistry ,lcsh:QD241-441 ,0404 agricultural biotechnology ,Antioxidant activity ,lcsh:Organic chemistry ,Flavonoid derivatives ,lcsh:Botany ,Drug Discovery ,medicine ,Cytotoxic T cell ,Pharmacology ,Traditional medicine ,Chemistry ,Organic Chemistry ,Fabaceae ,04 agricultural and veterinary sciences ,isoflavonoids ,040401 food science ,lcsh:QK1-989 ,0104 chemical sciences ,010404 medicinal & biomolecular chemistry ,lcsh:QD1-999 ,cytotoxicity - Abstract
Trifolium L. species with a rich isoflavone content have been used as expectorant, analgesic, antiseptic, tonic, and wound- healer in folk medicine. The aim of the study is to evaluate pharmacological properties of the extracts and isolated compounds of T. tricocephalum. Phytochemical investigation of the aerial parts of T. trichocephalum led to the isolation of daidzein, genistein, quercetin, and daidzein 4'-O-beta-glucoside for the first time from this species. Isolated compounds along with the methanol extract, water, ethyl acetate and chloroform subextracts were tested for their radical scavenging and cytotoxic activity which was evaluated by MTT assay. According to the results of activity tests, extracts showed a concentration-dependent radical scavenging activity as well as cytotoxic effect on HepG2 cells at 400 mu g/mL, whereas the compounds did not exert any obvious cytotoxic effect at tested concentrations.
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- 2017
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23. Phenolic Content And Enzyme Inhibition Activities Barbarea Auriculata Var. Paludosa, B. Integrifolia And B. Plantaginea (Brassicaceae)'S
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Merve Badem, Rezzan Aliyazicioglu, Sermet Yildirmis, Ali Kandemir, Sıla Ozlem Sener, Ufuk Özgen, Nuriye Korkmaz, and Seyda Kanbolat
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Barbarea ,chemistry.chemical_compound ,Chromatography ,chemistry ,Phytochemical ,biology ,Tyrosinase ,Ethyl acetate ,Coumaric acid ,biology.organism_classification ,High-performance liquid chromatography ,Syringaldehyde ,Benzoic acid - Abstract
Barbarea genus has been presented about 12 species, 9 taxon are endemic, in Turkey. In this sudy, enzyme inhibition was carried out on methanolic extract, chloroform extract, ethyl acetate extract, and the remaining aqueous phases from the aerial parts of B. auriculata var paludosa, B. integrifolia, and B. plantaginea species and HPLC studies were carried on their methanolic extract in the present study for the first time. Phenolic compounds were determined using reverse phase-high performance liquid chromatography (RP-HPLC). p-OH benzoic acid, vanilic acid, syringaldehyde, coumaric acid, synapic acid and benzoic acid were detected as major phenolic compounds in the species. Assay of enzyme inhibition activities were done using spectrophotometric methods. Results of these studies reveal that the extracts from these species have moderate tyrosinase, AChE and BuChE inhibitory activity. In the biological activity studies, it was observed that B. integrifolia was the highest activity.
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- 2020
24. İlaç ve Kozmetik Ürünler Teknolojisi
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Özlem Çoban and Ufuk Özgen
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- 2020
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25. Cholinesterase and Tyrosinase Inhibitory Potential, and Antioxidant Capacity of Lysimachia verticillaris L. and the Isolation of the Major Compounds
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Ufuk Özgen, Sıla Özlem Sener, Karel Šmejkal, Jiri Vaclavik, FATMA SEZER SENOL DENIZ, ILKAY ERDOGAN ORHAN, Emil Svajdlenka, Ahmet C Goren, and Milan ŽEMLIČKA
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Pharmaceutical Science ,Molecular Medicine - Published
- 2019
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26. FP306HYPERICUM PERFORATUM: A FRIEND OR A FOE FOR AMIKACIN INDUCED NEPHROPATHY
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Rezzan Aliyazicioglu, Ufuk Özgen, Gamze Çan, Sait Al, Seyda Akkaya, Oğuzhan Keskin, Sıla Ozlem Sener, Engin Yenilmez, Kubra Kaynar, and Nuriye Korkmaz
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Transplantation ,Nephrology ,business.industry ,Amikacin ,Medicine ,Pharmacology ,business ,medicine.disease ,Nephropathy ,medicine.drug - Published
- 2019
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27. Determination Of Phenolic Contents By Hplc, And Antioxidant, Antimicrobial, Antityrosinase, And Anticholinesterase Activities Of Psephellus Huber-Morathii
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Ali Kandemir, Ufuk Özgen, Merve Badem, Seyda Kanbolat, Rezzan Aliyazicioglu, Şengül Alpay Karaoğlu, Nuriye Korkmaz, Sıla Ozlem Sener, Nuray Balturk, RTEÜ, Fen - Edebiyat Fakültesi, Biyoloji Bölümü, and Karaoğlu, Şengül Alpay
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Psephellus ,Antimicrobia ,Antioxidant ,Psephellus huber-morathii ,Traditional medicine ,Chemistry ,medicine.medical_treatment ,medicine ,Antimicrobial ,High-performance liquid chromatography ,Anticholinesterase - Abstract
WOS: 000462169800004 The goal of our study was to examine of antioxidant, antimicrobial, anticholinesterase activities, and phenolic composition of Psephellus huber-morathii. the antioxidant activities of extracts have been assessed by Ferric reducing antioxidant power (FRAP), cupric reducing antioxidant capacity (CUPRAC), and 2,2-diphenylpicrylhydrazyl (DPPH) radical scavenging. Phenolic constituents were measured using reverse phase-high performance liquid chromatography (RP-HPLC), and antimicrobial activity was investigated using the agar well diffusion method. Total phenolic content, FRAP, and CUPRAC results of aqueous extract have been better than methanolic extract, except for DPPH activity. Benzoic acid, and p-coumaric acid as major phenolic compounds were specified. Methanolic extract was especially effective against all microorganisms tested except for Yersinia pseudotuberculosis. the methanolic extract have been displayed inhibitory effect on tyrosinase. All extracts have been exhibited lower acetylcholinesterase, and butyrylcholinesterase inhibitory activities than galantamine. P. huber-morathii can be considered in the food, and drug industries due to antioxidant capacity and antimicrobial activities of the species. It can be potential source as anti-browning agents because of its average tyrosinase inhibitory activity. Turkish Scientific and Technical Research CouncilTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) Merve Badem, Sila Ozlem Sener and Nuriye Korkmaz would like to acknowledge the scholarship by the Turkish Scientific and Technical Research Council.
- Published
- 2019
28. 4-(3,4-dihydroxybenzoyloxymethyl)phenyl-O-β-D-glucopyranoside effect in liver regeneration
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Tülin, Firat, Gizem, Söyler, Fatma, Töre, Mustafa, Şit, Aysu, Kiyan, Ufuk, Özgen, and Aysel, Kükner
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Male ,Hepatocytes ,Animals ,Hepatectomy ,Methylglucosides ,Rats, Wistar ,Cell Proliferation ,Liver Regeneration ,Rats - Abstract
Following an injury or resection, the mammalian liver has the capacity to regain its former volume and functioning by restoring itself. Studies have demonstrated that antioxidants play a role in hepatic regeneration. This study investigated the effect of 4-(3,4-dihydroxybenzoyloxymethyl) phenyl-O-β-D-glucopyranoside (PG) obtained from Origanum micranthum on liver regeneration. Sixty Wistar Albino rats were used. In the sham-operated group, a midline abdominal laparotomy was performed without hepatectomy. In the partial hepatectomy (PHx) group, the median and left lateral lobes were removed. Rats in the PHx group received 20 mg/kg/day PG intraperitoneally before being sacrificed at 24, 48, and 72 hrs, and 7 days later. Liver tissues were collected for immunohistochemical analysis and electron microscopic evaluation. We found an increase in mitotic index, and the numbers of Ki-67 stained hepatocytes in all PHx early stage groups (24 hr, 48hr, 72 hr), but not in 7-day groups. The regeneration mediators eNOS, iNOS, TNF-α and NF-κB were shown to increase in PHx groups. This increase was more prominent dependening on time. In the PHx treatment (PHx+PG) groups, while eNOS was still high, iNOS, TNF-α and NF-κB had decreased. The apoptotic index was markedly high in the PHx groups; this was prevented by PG treatment. These findings were supported by the ultrastructural results. Our findings indicate that PG supports liver regeneration, hepatocyte proliferation, reduced liver damage, and inflammatory mediators following PHx.
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- 2018
29. Evaluation of HPLC, phytochemical, anticholinesterase and antioxidant profiles of the aerial parts of Asperula taurina subsp. Caucasica
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Erhan Palaska, Ahmet C. Gören, Hatice Seçinti, Cavit Kazaz, Merve Badem, Seda Damla Hatipoğlu, Sıla Ozlem Sener, Ufuk Özgen, and GÖREN, AHMET CEYHAN
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antikolinesteraz aktivite,antioksidan aktivite,Asperula taurina subsp. caucasica,kersetin 3-O-β-galaktozit,parmak izi,Rubiaceae,YBSK ,Traditional medicine ,Phytochemical ,Farmakoloji ve Eczacılık ,General Medicine ,anticholinesterase activity,antioxidant activity,Asperula taurina subsp. caucasica,fingerprint,HPLC,quercetin 3-O-β-galactoside,Rubiaceae ,Biology ,Pharmacology and Pharmacy ,ÖZGEN U., ŞENER S. Ö. , Badem M., Seçinti H., HATIPOGLU S. D. , Gören A. C. , KAZAZ C., PALASKA E., -Evaluation of HPLC, phytochemical, anticholinesterase and antioxidant profiles of the aerial parts of Asperula taurina subsp. Caucasica-, Ankara Universitesi Eczacilik Fakultesi Dergisi, cilt.42, ss.1-13, 2018 ,High-performance liquid chromatography ,Asperula taurina - Abstract
Objective: In this study, we aimed to evaluate the HPLC, phytochemical, anticholinesterase and antioxidant profiles of the aerial parts of Asperula taurina subsp. caucasica.Material and Method: The fingerprint of the phenolic compounds of the methanolic extract of the plant was obtained using RP-HPLC method. The method was also validated in terms of detection limits, quantification limits, linearity, accuracy, precision and selectivity. The phenolic contents of A. taurina subsp. caucasica were detected as proto-catechuic acid, p-OH benzoic acid and benzoic acid. In the phytochemical studies, quercetin 3-O-β-galactoside was isolated from the ethyl acetate subfraction of A. taurina subsp. caucasica using by several chromatographic methods. The structure of the pure compound was elucidated by means of spectral analysis (1H NMR, 13C NMR, and ESI-MS). Anticholinesterase and antioxidant activity studies were performed on quercetin 3-O-β-galactoside and the methanolic extract of the plant.Result and Discussion: While quercetin 3-O-β-galactoside shown moderate inhibitory activity against butyrylcholinesterase at 200 μg/ml, quercetin3-O-β-galactoside and the metanolic exract of the plant did not show acetylcholinesterase inhibitory activity. Quercetin 3-O-β-galactoside shown DPPH free radical scavenging activity at 50 and 100 μg/ml, moderate lipid peroxidation inhibitory activity at 25, 50 and 100 μg/ml; the methanolic extract of the plant moderate lipid peroxidation inhibitory activity at 25, 50 and 100 μg/ml. In conclusion, A. taurina subsp. caucasica and quercetin 3-O-β-galactoside could be important and valuable sources for protecting our body health, especially Alzheimer’s disease., Amaç: Bu çalışmada, Asperula taurina subsp. caucasica'nın toprak üstü kısımlarının YBSK, fitokimyasal, antikolinesteraz ve antioksidan profillerinin değerlendirilmesi amaçlanmıştır.Gereç ve Yöntem: Bitkinin metanolik ekstresinin fenolik bileşiklerinin parmak izi kromatogramı, geliştirilen RP-YBSK yöntemi kullanılarak elde edilmiştir. YBSK yöntemi saptama limitleri, nicelik sınırları, doğrusallık, doğruluk, hassaslık ve seçicilik açısından valide edilmiştir. A. taurina subsp. caucasica fenolik içeriği, protokatekuik asit, p-OH benzoik asit ve benzoik asit olarak tespit edilmiştir. Fitokimyasal çalışmalarda ise, çeşitli kromatografik yöntemler kullanılarak A. taurina subsp. caucasica etil asetat alt fraksiyonundan kersetin 3-O-β-galaktozit izole edilmiştir. Saf bileşiğin yapısı, spektrum analizi (1H NMR, 13C NMR ve ESI-MS) yardımıyla aydınlatılmıştır. Kersetin 3-O-β-galaktozit ve bitkinin metanolik ekstresi üzerinde antikolinesteraz ve antioksidan aktivite çalışmaları yapılmıştır.Sonuç ve Tartışma: Kersetin 3-O-β-galaktozit, butirilkolinesteraz'a karşı 200 μg/ml'de orta düzeyde inhibe edici aktivite gösterirken, kersetin 3-O-β-galaktozit ve bitkinin metanolik ekstresi asetilkolinesteraz inhibitör etki göstermemiştir. Kersetin 3-O-β-galaktozit, 50 ve 100 μg/ml'de DPPH serbest radikal süpürücü aktivite, 25, 50 ve 100 μg/ml'de orta derecede lipit peroksidasyon inhibisyon aktivitesi gösterirken; bitkinin metanolik ekstresi 25, 50 ve 100 μg/ml'de orta düzeyde lipit peroksidasyonu önleyici aktivite göstermiştir. Sonuç olarak, A. taurina subsp. caucasica ve kersetin 3-O-β-galaktozit, özellikle Alzheimer hastalığından korunmada önemli ve değerli bir doğal ürün kaynağı olabilir.
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- 2018
30. Biological Screening Of Traditional Medicinal Plants From Villages Of Akkus (Ordu) In Turkey On The Effects Of Tyrosinase
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Serhat Sevgi, Merve Badem, Maksut Coşkun, Seyda Kanbolat, Nuriye Korkmaz, Rezzan Aliyazicioglu, Sıla Ozlem Sener, and Ufuk Özgen
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Folk medicine ,Traditional medicine ,Food animal ,Tyrosinase ,Ornamental plant ,fungi ,food and beverages ,Biology ,Medicinal plants ,Inhibitory effect - Abstract
In the present study, ethnomedicinal uses of the plants naturally growing in 35 villages of Akkuş District have been determined. Informations about the uses of the plants have been obtained from villagers using a questionnaire. At the end of the identification of the plants collected from villages, 58 taxa belong to 32 families have been determined. Furthermore, tyrosinase enzyme activity studies of methanolic extracts of all the species carried out spectroscopicallly. Eventually, the plants has been demonstrated to be used as folk medicine, food animal feed, ornamental, stuff, dyeing, odour, insecticide and fishing. According to the biological screening studies, some species have been indicated to have inhibitory effect, but the others have activator effect on tyrosinase enzyme.
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- 2018
31. Phtytochemical Studies and Quantitative HPLC Analysis of Rosmarinic Acid and Luteolin 5-O-β-D-Glucopyranoside on Thymus praecox subsp. grossheimii var. grossheimii
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Cavit Kazaz, Hayri Duman, Handan G. Sevindik, Alptug Atila, Ufuk Özgen, and Handan Ozturk Er
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chemistry.chemical_classification ,Traditional medicine ,biology ,Rosmarinic acid ,General Chemistry ,General Medicine ,biology.organism_classification ,Flavones ,chemistry.chemical_compound ,Biochemistry ,chemistry ,Ursolic acid ,Phytochemical ,Drug Discovery ,Lamiaceae ,Thymus Plant ,Oleanolic acid ,Luteolin - Abstract
Thymus praecox ssp. grossheimii (RONNIGER) JALAS var. grossheimii (Lamiaceae) is used as an herbal tea for cold, stomachache, cough, and infections in Turkey. There are no phytochemical studies on this species. We performed phytochemical studies and quantitative analysis of rosmarinic acid and luteolin 5-O-β-D-glucopyranoside in the methanol extract of the plant. Several chromatographic methods were used for the isolation of major compounds. HPLC methods were applied for quantitative analysis of rosmarinic acid and luteolin 5-O-β-D-glucopyranoside in the methanol extract. In this study, ursolic acid (1), oleanolic acid (2), methyl rosmarinate (3), ethyl rosmarinate (4), rosmarinic acid (5), luteolin 5-O-β-D-glucopyranoside (6), and thymoquinol 2,5-O-β-diglucopyranoside (7) were isolated from the aerial parts of the plant. The relative contents of rosmarinic acid and luteolin 5-O-β-D-glucopyranoside in the extract were 15.2 and 57.8 mg/g of dry weight, respectively. Compounds isolated from this plant and the contents of rosmarinic acid and luteolin 5-O-β-D-glucopyranoside provided reasonable evidence for the traditional usages of this plant.
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- 2015
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32. Phytochemical Studies on Origanum rotundifolium
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Ufuk Özgen, Cavit Kazaz, and Esen Sezen Karaoglan
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Pharmacology ,Origanum rotundifolium ,lcsh:Therapeutics. Pharmacology ,Traditional medicine ,Phytochemical ,Phytochemical study ,lcsh:RM1-950 ,Biology ,biology.organism_classification - Published
- 2017
33. Investigation of Phenolic Compounds by RP-HPLC and Antioxidant, Antimicrobial, Tyrosinase Inhibitor Activities of Clinopodium vulgare L. subsp. vulgare Extract
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Seyda Akkaya, Rezzan Aliyazicioglu, Şengül Alpay Karaoğlu, Ufuk Özgen, Nuriye Korkmaz, and Sıla Ozlem Sener
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Engineering ,Traditional medicine ,Chemistry ,Antioksidan,Antimikrobiyal,Tirozinaz İnhibitör Aktivite,HPLC ,Antioxidants,Antimicrobial,Tyrosinase inhibition,HPLC ,Mühendislik ,Clinopodium vulgare - Abstract
Clinopodium vulgare L. subsp. vulgare, Clinopodiumcinsine ve Lamiaceae familyasına mensup çok yıllık aromatik otsu bir bitkidir.Bu cins bitkiler sıklıkla geleneksel tedavide kullanılmaktadır. FarklıClinopodium türlerinin farklı ekstrelerinin antioksidan, antimikrobiyal,tirozinaz inhibitör etkilerini ve HPLC ile fenolik bileşenlerinin analizinikonu alan çalışmalar olmasına rağmen, Clinopodium vulgare L. subsp. vulgare ekstresininbu özelliklerinin tümünü içeren herhangi bir çalışma bulunmamaktadır. Buçalışmanın amacı C. vulgare subsp.vulgare metanol ekstresinin antioksidan, antimikrobiyal, tirozinaz inhibitöraktivitelerini ve HPLC analizi ile fenolik madde içeriğini belirlemektir. Ekstrenin fenolikbileşenleri ters faz yüksek performanslı sıvı kromatografisi (RP-HPLC) ilebelirlendi. Antioksidan, tirozinaz inhibitör aktivite spektrofotometrikyöntemlerle ve antimikrobiyal aktivite disk difüzyon metodu ile incelendi. Ekstrenin toplam fenolik madde miktarı 27.9±0.4 mg gallikasit eşdeğeri/g numune, 2,2-difenil-1-pikrilhidrazil (DPPH) radikal süpürme kapasitesiIC50 değeri 0.114±0.0004 mg/mL ve ferrik indirgeyici antioksidan güç(FRAP) değeri 1556±3 µM troloks eşdeğeri/g numune olarak hesaplandı. Tirozinazinhibisyon çalışma sonucuna göre ekstrenin IC50 değeri kojik asitstandardından yüksek bulundu. HPLC ile analiz sonucunda protokatekuik asit,klorojenik asit, vanilin, sinapik asit ve benzoik asit tespit edildi. Ekstre,asid-hızlı bakteri (M. smegmatis), bazı gram pozitif (S. aureus ve B. cereus)ve bazı gram negatif (Y. pseudotuberculosis) bakterilere karşı ılımlıantibakteriyal aktivite gösterdi. Ancak C. albicans and S. cerevisiae türlerinekarşı hiçbir antifungal aktivite göstermedi. Bu çalışmanın sonuçlarına göre, C. vulgare subsp. vulgare yenifarmasotiklerin geliştirilmesinde potansiyel bir kaynak olarak düşünülebilir., Clinopodium vulgare L. subsp. vulgare is a perennialaromatic herbaceous plant belonging to Clinopodium genus and Lamiaceae family.This genus plants are often used in traditional therapy. There are no studiesinvolving the whole of these properties of the Clinopodium vulgare L. subsp.vulgare extract, although there are studies about antioxidant, antimicrobial,tyrosinase inhibitor effects and analysis of phenolic compounds by HPLC ofdifferent extracts of different Clinopodium genus. The purpose of this studywas to determine the phenolic composition by HPLC, and antioxidant,antimicrobial, and tyrosinase inhibitor activity of methanolic extract of C. vulgare subsp. vulgare. The phenolic compounds were determined by reversephase high performance liquid chromatography (RP-HPLC). The antioxidant, tyrosinaseinhibitor, and antimicrobial activities of the extract were examined by spectrophotometricmethods, and disc diffusion method, respectively. The total phenolic content(TPC), 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (DPPH), andferric reducing antioxidant power (FRAP) values of the extract were found27.9±0.4 mg gallic acid equivalents per g sample, 0.114±0.0004 mg/mL, and 1556±3µM trolox equivalents per g sample, respectively. IC50 value of the extract according to thetyrosinase inhibitition study results was found higher than kojic acid standard.The protocatechuic acid, chlorogenic acid, vanillin, sinapic acid, benzoic acidwere detected by HPLC analysis. The extract exhibited while moderateantibacterial activity against an acid-fast bacterium (M. smegmatis), some grampozitive (S. aureus and B. cereus) and gram negative (Y. pseudotuberculosis)bacteria. But, antifungal activity was not showed against C. albicans and S. cerevisiae species. According tothe results of this study, C. vulgare subsp. vulgare can beconsidered as a potential source for developing new pharmaceuticals.
- Published
- 2017
34. Phytochemical Studies on Origanum acutidens
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Esen Sezen Karaoglan, Cavit Kazaz, and Ufuk Özgen
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Traditional medicine ,Phytochemical ,Chemistry ,Origanum acutidens - Published
- 2017
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35. Composition of Volatile Components and Biological Activities of Aethionema diastrophis
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Şengül Alpay Karaoğlu, Rezzan Aliyazicioglu, Ozan Emre Eyupoglu, and Ufuk Özgen
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0106 biological sciences ,0301 basic medicine ,biology ,Chemistry ,Plant Science ,General Chemistry ,030108 mycology & parasitology ,biology.organism_classification ,01 natural sciences ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,Environmental chemistry ,Aethionema ,Composition (visual arts) ,010606 plant biology & botany - Published
- 2017
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36. Relationship Between Chemical Structure and Antioxidant Activity of Luteolin and Its Glycosides Isolated from T . sipyleus subsp. sipyleus var. sipyleus
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Ufuk Özgen, Ahmet Mavi, Zeynep Terzi, Cavit Kazaz, Ali Aşçı, Yusuf Kaya, and Hasan Seçen
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lcsh:Chemistry ,lcsh:QD241-441 ,Lamiaceae ,Thymus sipyleus subsp. sipyleus var. sipyleus ,lcsh:QD1-999 ,lcsh:Organic chemistry ,antioxidant activity ,lcsh:Botany ,luteolin glycosides ,luteolin ,lcsh:QK1-989 - Abstract
One triterpenic acid (ursolic acid), one phenolic acid (rosmarinic acid), and four flavonoids (luteolin, luteolin 7-O-(6”-feruloyl)- b -glucopyranoside, luteolin 5-O- b -glucopyranoside, and luteolin 7-O- b -glucuronide) were isolated from the aerial parts of Thymus sipyleus subsp. sipyleus var. sipyleus and identified by spectroscopic methods. In vitro lipid peroxidation inhibition effects of the compounds were determined using TBA test method in a bovine brain liposome system. All compounds inhibited lipid peroxidation in various degrees except for ursolic acid. The order of the lipid peroxidation activities of luteolin and its glycosides were: Luteolin 7-O- b -glucuronide> luteolin 5-O- b -glucopyranoside> luteolin 7-O-(6”-feruloyl)- b -glucopyranoside > rosmarinic acid >luteolin. However, the activity order of the compounds was completely different in DPPH radical-scavenging activity. None of the compounds shows Fe chelating activity. The results were discussed based on their chemical structures and polarities.
- Published
- 2011
37. Memory-Vitalizing Effect Of Twenty-Five Medicinal And Edible Plants And Their Isolated Compounds
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Leyla Guven, Krystyna Skalicka-Wozniak, Ufuk Koca Çalişkan, F. Sezer Senol, Gülin Renda, Nazim Sekeroglu, Ufuk Özgen, Ilkay Erdogan Orhan, Handan G. Sevindik, Esen Sezen Karaoglan, and Gokhan Bulut
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biology ,010405 organic chemistry ,Chemistry ,DPPH ,Tyrosinase ,Onosma ,Ethyl acetate ,Plant Science ,Origanum ,Salvia ,biology.organism_classification ,01 natural sciences ,0104 chemical sciences ,010404 medicinal & biomolecular chemistry ,chemistry.chemical_compound ,Prunus ,Botany ,Hypericum - Abstract
The extracts from 25 plant species from Salvia, Hypericum, Onosma, Thymus, Origanum, Rosa, and Prunus with eight isolated compounds were screened for their memory-vitalizing potential against the enzymes, i.e., acetyl cholinesterase (AChE), butyrylcholinesterase (BChE), and tyrosinase (TYR) along with DPPH, DMPD, and NO radicals and iron-chelation capacity using spectrophotometric microtiter assays. The n-hexane:dichloromethane (1:1) extract of Onosma nigricaule roots (63.18 +/- 0.56%) and the ethyl acetate extract of the aerial parts of Hypericum capitatum var. capitatum (50.79 +/- 3.88%) were the most active toward AChE and BChE, respectively. The highest iron-chelating capacity was found in the n-hexane extract of Thymus sipyleus subsp. sipyleus var. sipyleus, where mostly Labiatae species exhibited better radical scavenging effect. Among the extracts, the roots of O. nigricaule, the aerial parts of Hypericum capitatum var. capitatum and T. sipyleus subsp. sipyleus var. sipyleus could be further evaluated for their memory-vitalizing properties based on different mechanisms. (C) 2015 SAAB. Published by Elsevier B.V. All rights reserved.
- Published
- 2016
38. Effects of Onosma armeniacum root extract on ethanol-induced oxidative stress in stomach tissue of rats
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Aysegul Koc, Hülya Aksoy, Ufuk Özgen, Zekai Halici, Halis Suleyman, Nurinnisa Ozturk, and Elif Cadirci
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Antioxidant ,medicine.medical_treatment ,Pharmacology ,Nitric Oxide ,Ranitidine ,Toxicology ,medicine.disease_cause ,Plant Roots ,Thiobarbituric Acid Reactive Substances ,Linoleic Acid ,Lipid peroxidation ,Superoxide dismutase ,chemistry.chemical_compound ,Malondialdehyde ,medicine ,Animals ,Stomach Ulcer ,Rats, Wistar ,Glutathione Transferase ,Peroxidase ,Ethanol ,biology ,Plant Extracts ,Superoxide Dismutase ,Stomach ,General Medicine ,Glutathione ,Boraginaceae ,Catalase ,Rats ,Oxidative Stress ,medicine.anatomical_structure ,chemistry ,Biochemistry ,Gastric Mucosa ,biology.protein ,Lipid Peroxidation ,Oxidative stress ,Phytotherapy ,medicine.drug - Abstract
This study investigated the effects of Onosma armeniacum K. (Boraginaceae) root extract (AR-1) on ethanol-induced stomach ulcers, and on some oxidant and antioxidant parameters, in stomach tissue in rats. The results obtained showed that AR-1 significantly inhibited ethanol-induced ulcers at 25, 50, 100 and 200 mg/kg doses. We found that 50, 100 and 200 mg/kg doses of AR-1 inhibited ulcers more effectively than did ranitidine. AR-1 at doses of 25, 50, 100 and 200 mg/kg significantly prevented the decrease in total glutathione (tGSH) level which occurs in damaged stomach tissues of rats given ethanol (control group). Only a 100 mg/kg dose of AR-1 significantly increased the glutathione S-transferase (GST) level in stomach tissue compared to the control. All doses of AR-1 except the 25 mg/kg dose eliminated the decrease in the superoxide dismutase (SOD) level in the stomach tissue of rats given ethanol. While all doses of AR-1 decreased malondialdehyde (MDA) levels significantly; all doses AR-1 except 25 mg/kg decreased myeloperoxidase (MPO) levels significantly compared to the control. The effect of AR-1 on catalase (CAT) activity was insignificant at all doses. AR-1 significantly increased nitric oxide (NO) levels at 50, 100 and 200 mg/kg doses compared to the control. Our results indicate that the protection of some antioxidant mechanisms and the inhibition of some oxidant mechanisms have a role in AR-1's antiulcer effect mechanism.
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- 2007
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39. Anodic voltammetric behavior of ascorbic acid and its selective determination in pharmaceutical dosage forms and some Rosa species of Turkey
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Bengi Uslu, Sibel A. Ozkan, Maksut Coşkun, Ufuk Özgen, C. S. Erdurak-Kiliç, and Burcu Dogan
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Detection limit ,Chromatography ,Chemistry ,Analytical chemistry ,Square wave ,Differential pulse voltammetry ,Electrochemistry ,Ascorbic acid ,High-performance liquid chromatography ,Voltammetry ,Dosage form ,Analytical Chemistry - Abstract
Ascorbic acid is the most common electroactive biological compound found in some plant species (e.g., Citrus species, Rosa species). The electrochemical oxidation of ascorbic acid was investigated by cyclic, linear sweep, differential pulse (DPV), and square wave (SWV) voltammetry. For analytical purposes, a very well-resolved diffusion-controlled voltammetric peak was obtained in acetate buffer at pH 3.50 for DPV and SWV. The linear response was obtained in the range of 3.52–176.1 μg/mL with a detection limit of 0.88 μg/mL for DPV and 0.52 µg/mL for SWV. Based on this study, simple, rapid, selective, and sensitive voltammetric methods were developed for the determination of ascorbic acid in pharmaceutical dosage forms and Rosa species (R. dumalis ssp. boissieri var. boissieri, R. canina, R. pulverulenta, R. heckeliana ssp. vanheurckiana, and R. montana subsp. woronowii). The results obtained are compared with the HPLC data. The developed methods enable the extracts to be analyzed without the necessity of any time-consuming separation.
- Published
- 2006
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40. Fibroblast growth stimulation by extracts and compounds of Onosma argentatum roots
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M Ikbal, Peter J. Houghton, Ufuk Özgen, Fatma Göçer, Hasan Dogan, Ahmet Hacimuftuoglu, and Maksut Coşkun
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Onosma ,Pharmacognosy ,Plant Roots ,Drug Discovery ,medicine ,Humans ,Amnion ,Growth Substances ,Cytotoxicity ,Fibroblast ,Cells, Cultured ,Cell Proliferation ,Pharmacology ,Dose-Response Relationship, Drug ,biology ,Traditional medicine ,Plant Extracts ,Boraginaceae ,Fibroblasts ,biology.organism_classification ,In vitro ,medicine.anatomical_structure ,Biochemistry ,Wound healing - Abstract
The roots of Onosma argentatum are used traditionally in Turkey for wound healing and burns. The n-hexane-dichloromethane extract of the roots, and four shikonin derivatives (deoxyshikonin, acetyl shikonin, 3-hydroxy-isovaleryl shikonin and 5,8-O-dimethyl acetyl shikonin) isolated from the n-hexane-dichloromethane extract were investigated for their ability to stimulate the growth of human amnion fibroblasts. A range of concentrations was studied and the extract found to stimulate the growth of human amnion fibroblasts in vitro at 0.1 microg/mL whilst 5,8-O-dimethyl acetyl shikonin had the same effect at 0.05-5 microg/mL, although cytotoxicity was observed at 50 microg/mL for all samples. The extract and all the other isolated compounds showed cytotoxicity at 10 microg/mL with the extract and 3-hydroxy-isovaleryl shikonin showing cytotoxicity at 5 microg/mL. It is suggested that any wound healing effect of the roots of Onosma argentatum might be partly due to an additive effect of the shikonin derivatives present.
- Published
- 2006
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41. Antioxidant Properties of Some Medicinal Lamiaceae (Labiatae) Species
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Zeynep Terzi, Peter J. Houghton, Ufuk Özgen, Ahmet Mavi, Ali Yildirim, and Maksut Coşkun
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Pharmacology ,Antioxidant ,biology ,Traditional medicine ,DPPH ,medicine.medical_treatment ,Pharmaceutical Science ,Decoction ,General Medicine ,Pharmacognosy ,Ascorbic acid ,biology.organism_classification ,Teucrium ,chemistry.chemical_compound ,Complementary and alternative medicine ,chemistry ,Drug Discovery ,Botany ,medicine ,Molecular Medicine ,Lamiaceae ,Mentha longifolia - Abstract
In this study, antioxidant properties of Thymus sipyleus. Boiss. subsp. sipyleus. var. sipyleus., Teucrium chamaedrys L.., Mentha longifolia. (L.) Hudson subsp. longifolia., Salvia limbata. C.A. Meyer, and Thymus fallax. Fisch. & Mey. were investigated. Antioxidant and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities, reducing powers, and the amount of total phenolic compounds of the extracts were studied. The highest antioxidant activity was shown by T. chamaedrys. (decoction, IC50: 9.2 µg/ml), and the lowest one was S. limbata. (decoction, IC50: 619.5 µg/ml). The highest DPPH radical scavenging activity was shown by T. fallax. [decoction, IC50S: 56 µg/ml (IC50S is the extract concentration (µg/ml) required for 50% inhibition of the DPPH solution absorbance at 517 nm)] while the lowest one was S. limbata. (decoction, IC50S: 335.8 µg/ml). The highest reducing power and amount of total phenolic compounds was shown by T. chamaedrys. (decoction, 29.9 µg/ml ascorbic acid equivalent, ...
- Published
- 2006
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42. ANTIOXIDANT ACTIVITIES AND TOTAL PHENOLIC COMPOUNDS AMOUNT OF SOME ASTERACEAE SPECIES
- Author
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Ufuk Özgen, Ahmet Mavi, Zeynep Terzi, Maksut Coskun, and Ali Yildirim
- Subjects
Antioksidan ,radikal süpürücü ,lcsh:Pharmacy and materia medica ,fenolik bilesik ,lcsh:RS1-441 ,indirgeme gücü ,Asteraceae - Abstract
Antioxidant and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities, reducingpow-ers and the amount of total phenolic compounds of some medicinal Asteraceae species used in folk med-icine in Eastern Anatolia were studied. These species are Achillea biebersteinii, Achillea wilhelmsii, Artemisia absinthium, Artemisia austriaca, Cichorium intybus, Helichrysum arenarium subsp. rubicun-dum, Tripleurospermum oreades var. oreades. The highest antioxidant activity is shown by methanol extract of A. austriaca followed by water extract of A. austriaca, methanol extract of A. wilhelmsii, water extract of H. arenarium, water extract of A. biebersteinii, water extract of A. absinthium, water extract of C. intybus, water extract of A. wilhelmsii, water extract of T. oreades. The highest DPPH radical scavenging activity is also shown by methanol extract of A. austriaca followed by water extract of A. austriaca, water extract of H. arenarium, water extract of A. absinthium, water extract of A. bieber-steinii, water extract of C. intybus, water extract of A. wilhelmsii, water extract of T. oreades, methanol extract of A. wilhelmsii. Özet Dogu Anadolu’da halk arasinda tedavi amaciyla kullanilan bazi Asteraceae türlerinin antioksidan, 2,2-difenil-1-pikrilhidrazil (DPPH) radikal süpürücü aktiviteleri, indirgeme güçleri ve total fenolik bilesik miktarlari arastirilmistir. Bu türler, Achillea biebersteinii, Achillea wilhelmsii, Artemisia absinthium, Artemisia austriaca, Cichorium intybus, Helichrysum arenarium subsp. rubicundum ve Tripleurospermum oreades var. oreades’den olusmaktadir. En yüksek antioksidan aktivite A. austria-ca’niin methanol ekstresinde gözlenmistir; bunu sirasiyla A. austriaca su ekstresi, A. wilhelmsii metanol ekstresi, H. arenarium su ekstresi, A. biebersteinii su ekstresi, A. absinthium su ekstresi, C. intybus su ekstresi, A. wilhelmsii sulu ekstresi ve T. oreades sulu ekstresi izlemektedir. En yüksek DPPH radikal süpürücü aktivite A. austriaca’nin methanol ekstresinde gözlenmistir; bunu sirasiyla A. austriaca su ekstresi, H. arenarium su ekstresi, A. absinthium su ekstresi, A. biebersteinii su ekstresi, C. intybus su ekstresi, A. wilhelmsii su ekstresi, T. oreades su ekstresi ve A. wilhelmsii metanol ekstresi izlemektedir.
- Published
- 2004
43. Carbonic Anhydrase and Urease Inhibitory Potential of Various Plant Phenolics Using in vitro and in silico Methods
- Author
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Abdur Rauf, Ilkay Erdogan Orhan, Muslim Raza, Esen Sezen Karaoglan, Gülin Renda, Ufuk Özgen, Erhan Palaska, and Muhammad Saleem
- Subjects
Urease ,Bioengineering ,01 natural sciences ,Biochemistry ,Structure-Activity Relationship ,chemistry.chemical_compound ,Phenols ,Catalytic Domain ,Carbonic anhydrase ,medicine ,Computer Simulation ,Carbonic Anhydrase Inhibitors ,Molecular Biology ,IC50 ,biology ,Plant Extracts ,010405 organic chemistry ,Active site ,General Chemistry ,General Medicine ,0104 chemical sciences ,Molecular Docking Simulation ,010404 medicinal & biomolecular chemistry ,chemistry ,Docking (molecular) ,Apigenin ,biology.protein ,Molecular Medicine ,Acetazolamide ,Luteolin ,Protein Binding ,medicine.drug - Abstract
Plant phenolics are known to display many pharmacological activities. In the current study, eight phenolic compounds, e.g., luteolin 5-O-beta-glucoside (1), methyl rosmarinate (2), apigenin (3), vicenin 2 (4), lithospermic acid (5), soyasaponin II (6), rubiadin 3-O-beta-primeveroside (7), and 4-(beta-D-glucopyranosyloxy) benzyl 3,4-dihydroxybenzoate (8), isolated from various plant species were tested at 0.2 mM against carbonic anhydrase-II (CA-II) and urease using microtiter assays. Urease inhibition rate for compounds 1-8 ranged between 5.0-41.7%, while only compounds 1, 2, and 4 showed a considerable inhibition over 50% against CA-II with the IC50 values of 73.5 +/- 1.05, 39.5 +/- 1.14, and 104.5 +/- 2.50 mu M, respectively, where IC50 of the reference (acetazolamide) was 21.0 +/- 0.12 mu M. In silico experiments were also performed through two docking softwares (Autodock Vina and i-GEMDOCK) in order to find out interactions between the compounds and CA-II. Actually, compounds 6 (30.0%) and 7 (42.0%) possessed a better binding capability toward the active site of CA-II. According to our results obtained in this study, among the phenolic compounds screened, particularly 1, 2, and 4 appear to be the promising inhibitors of CA-II and may be further investigated as possible leads for diuretic, anti-glaucoma, and antiepileptic agents.
- Published
- 2017
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44. Folk medicines in the villages of Ilıca District (Erzurum, Turkey)
- Author
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Yusuf Kaya, Peter J. Houghton, and Ufuk Özgen
- Subjects
Key words: Folk medicine,Ilıca District,Erzurum,Turkey ,Physiology ,Stomach disorder ,Genetics ,Plant species ,Cell Biology ,Biology ,General Agricultural and Biological Sciences ,Socioeconomics ,Animal species ,Molecular Biology ,Microbiology - Abstract
The villages of Ilıca District in Erzurum Province, Turkey, were surveyed in terms of plants and animals used traditionally for medicinal purposes. Plants were compiled according to their families, botanical names, local names, usages, and applications. A total of 70 plant species belonging to 29 families are used by village people to treat a wide range of complaints, such as hemorrhoids, urinary system infections, stomach disorders, respiratory diseases, skin diseases, rheumatism, and jaundice. In addition, 3 animal species are used for the treatment of some diseases. The collected data were compared with those formerly recorded.
- Published
- 2014
45. ASPERULA TAURİNA L. SUBSP. CAUCASİCA (POBED.) EHREND. (RUBİACEAE) ÜZERİNDE MORFOLOJİK VE ANATOMİK ÇALIŞMALAR : MORPHOLOGICAL AND ANATOMICAL STUDIES ON ASPERULA TAURİNA L. SUBSP. CAUCASICA (POBED.) EHR
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Maksut Coşkun and Ufuk Özgen
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Pharmacology ,Rubiaceae ,Botany ,Pharmaceutical Science ,Biology ,biology.organism_classification ,Asperula taurina - Published
- 1999
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46. SARMISAK ve BAZI ALLIUM TÜRLERİNİN ANTİMİKROBİK ETKİLERİ ÜZERİNE KARŞILAŞTIRMALI BİR ÇALIŞMA : A COMPARATIVE STUDY ON ANTIMICROBIAL ACTIVITIES OF GARLIC AND SOME ALLIUM SPECIES
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Mehmet Koyuncu, Mete Babacan, Rana Taşkin, Ufuk Özgen, and Erdal Tuncel
- Subjects
Pharmacology ,biology ,Botany ,Pharmaceutical Science ,Allium ,biology.organism_classification ,Antimicrobial - Published
- 1997
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47. TÜRKİYE'DE YETİŞEN SAMBUCUS TÜRLERİNİN MEYVALARINDA ANTOSİYANOZİT TEŞHİSİ VE MİKTAR TAYİNİ : QUALITATIVE AND QUANTITATIVE DETERMINATION OF ANTHOCYANIN CONTENT IN THE FRUITS OF SAMBUCUS SPECIES GROWI
- Author
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Ufuk Özgen and Mualla Yenen
- Subjects
Pharmacology ,Horticulture ,chemistry.chemical_compound ,biology ,chemistry ,Anthocyanin ,Botany ,Sambucus ,Pharmaceutical Science ,biology.organism_classification ,Quantitative determination - Published
- 1997
- Full Text
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48. Norsesterterpenes and diterpenes from the aerial parts of Salvia limbata
- Author
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Gulacti Topcu, C. Eris, Ufuk Sönmez, Ayhan Ulubelen, and Ufuk Özgen
- Subjects
Plant Science ,General Medicine ,Horticulture ,Biology ,Salvia limbata ,Biochemistry ,Terpenoid ,Sesterterpenes ,chemistry.chemical_compound ,chemistry ,Botany ,Diterpene ,Spectral data ,Molecular Biology - Abstract
From the aerial parts of Salvia limbata, two new diterpenes (limbinal and acetyllimbinol) and two new dinorsesterterpenes (6-dehydroxy-yosgadensonol and 6-dehydroxy-13-epi-yosgadensonol) were isolated in addition to eight known terpenoids and four flavonoids. The structures of the new and the known compounds were established by spectral data.
- Published
- 1996
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49. Pytochemical Screening of the Specimens from İdris Mountain (Turkey) ll
- Author
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Nevin TANKER, Mehmet KOYUNCU, Maksut COŞKUN, Ayşegül GÜVEN, and Ufuk ÖZGEN
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General Medicine - Published
- 1996
- Full Text
- View/download PDF
50. Biological Activity and Characterization of Volatile Compounds ofTeucrium orientaleVar.glabrescensby SPME and GC-FID/MS
- Author
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Rezzan Aliyazicioglu, Sermet Yildirmis, Ozan Emre Eyupoglu, Şengül Alpay Karaoğlu, and Ufuk Özgen
- Subjects
Pharmacology ,Antioxidant ,Traditional medicine ,biology ,010405 organic chemistry ,Nonanal ,medicine.medical_treatment ,Rosmarinic acid ,Biophysics ,Cell Biology ,biology.organism_classification ,Solid-phase microextraction ,Antimicrobial ,01 natural sciences ,0104 chemical sciences ,Teucrium ,010404 medicinal & biomolecular chemistry ,chemistry.chemical_compound ,chemistry ,Botany ,medicine ,Agar diffusion test ,Medicinal plants ,Food Science - Abstract
This study describes the constituents of phenolic acids, volatile components, antioxidant and antimicrobial activities of Teucrium orientale var. glabrescens. Antioxidant activity of methanolic and aquous extracts were examined by three different methods, namely ferric reducing antioxidant power, cupric reducing antioxidant capacity and 2,2-diphenylpicrylhydrazyl radical scavenging activity. The phenolic constituents were also determined by reverse phase-high performance liquid chromatography. Solid phase microextraction of Teucrium orientale var. glabrescens was analyzed by Gas Chromatography-Flame Ionization Dedector/Mass Spectromerty, Solid-Phase Microextraction (GC-FID/MS, SPME). The antimicrobial activity was studied by agar diffusion method. Extracts showed strong antioxidant and antimicrobial activity and contained large amounts of antioxidant compounds. Rosmarinic acid, p-coumaric acid and p-hydroxybenzoic acid were detected in methanolic extract of plant. Aquous extract was especially effective against Mycobacterium smegmatis. The main chemical classes identified consisted of aldehydes (32.5%), hydrocarbons (29.8%) and monoterpene hydrocarbons (29.8%). Common volatile components of plant detected included nonanal, thuja-2,4 (10)-diene and tetracosane. Practical Applications Medicinal plants are natural resources used in the treatment of various ailments. Teucrium species have been widely used in folk medicine as a remedy for gastritis, parasitic worms, colds, digestive ulcers, etc. Teucrium species are the subject of growing interest because of their biological properties. Although studies on screening the antimicrobial, and antioxidant activity of the Teucrium species have been done densely, studies on phenolic compounds of Teucrium orientale var. glabrescens are very limited. Teucrium orientale var. glabrescens extracts studied proved to be a good source of antioxidants, antimicrobial agents and volatile components that might serve to protect health and fight against several diseases.
- Published
- 2016
- Full Text
- View/download PDF
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