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20 results on '"Uenalan, Murat"'

1. Cooperativity of RUNX1 and CSF3R mutations in severe congenital neutropenia: a unique pathway in myeloid leukemogenesis

Author

Skokowa, Julia, Steinemann, Doris, Katsman-Kuipers, Jenny E., Zeidler, Cornelia, Klimenkova, Olga, Klimiankou, Maksim, Ünalan, Murat, Kandabarau, Siarhei, Makaryan, Vahagn, Beekman, Renee, Behrens, Kira, Stocking, Carol, Obenauer, Julia, Schnittger, Susanne, Kohlmann, Alexander, Valkhof, Marijke G., Hoogenboezem, Remco, Göhring, Gudrun, Reinhardt, Dirk, Schlegelberger, Brigitte, Stanulla, Martin, Vandenberghe, Peter, Donadieu, Jean, Zwaan, C. Michel, Touw, Ivo P., van den Heuvel-Eibrink, Marry M., Dale, David C., and Welte, Karl

Published
2014
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3. LEF-1 is crucial for neutrophil granulocytopoiesis and its expression is severely reduced in congenital neutropenia

Author

Skokowa, Julia, Cario, Gunnar, Uenalan, Murat, Schambach, Axel, Germeshausen, Manuela, Battmer, Karin, Zeidler, Cornelia, Lehmann, Ulrich, Eder, Matthias, Baum, Christopher, Grosschedl, Rudolf, Stanulla, Martin, Scherr, Michaela, and Welte, Karl

Abstract

Author(s): Julia Skokowa (corresponding author) [1]; Gunnar Cario [1, 5, 6]; Murat Uenalan [1, 6]; Axel Schambach [1, 2]; Manuela Germeshausen [1]; Karin Battmer [2]; Cornelia Zeidler [1]; Ulrich Lehmann [...]

Published
2006
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4. Ultra-Sensitive CSF3R Deep Sequencing in Patients With Severe Congenital Neutropenia

Author

Klimiankou, Maksim, Uenalan, Murat, Kandabarau, Siarhei, Nustede, Rainer, Steiert, Ingeborg, Mellor-Heineke, Sabine, Zeidler, Cornelia, Skokowa, Julia, and Welte, Karl

Subjects
Male, lcsh:Immunologic diseases. Allergy, Neutropenia, Adolescent, Carcinogenesis, Immunology, DNA Mutational Analysis, G-CSFR mutations, Polymorphism, Single Nucleotide, Severity of Illness Index, leukemogenesis, hemic and lymphatic diseases, Receptors, Colony-Stimulating Factor, Immunology and Allergy, Congenital Bone Marrow Failure Syndromes, Humans, Child, Early Detection of Cancer, Original Research, deep-sequencing, High-Throughput Nucleotide Sequencing, pre-leukemia, Leukemia, Myeloid, Acute, severe congenital neutropenia, Myelodysplastic Syndromes, Mutation, Disease Progression, Female, lcsh:RC581-607
Abstract

High frequency of acquired CSF3R (colony stimulating factor 3 receptor, granulocyte) mutations has been described in patients with severe congenital neutropenia (CN) at pre-leukemia stage and overt acute myeloid leukemia (AML) or myelodysplastic syndrome (MDS). Here, we report the establishment of an ultra-sensitive deep sequencing of a CSF3R segment encoding the intracellular “critical region” of the G-CSFR known to be mutated in CN-MDS/AML patients. Using this method, we achieved a mutant allele frequency (MAF) detection rate of 0.01%. We detected CSF3R mutations in CN patients with different genetic backgrounds, but not in patients with other types of bone marrow failure syndromes chronically treated with G-CSF (e.g., Shwachman-Diamond Syndrome). Comparison of CSF3R deep sequencing results of DNA and cDNA from the bone marrow and peripheral blood cells revealed the highest sensitivity of cDNA from the peripheral blood polymorphonuclear neutrophils. This approach enables the identification of low-frequency CSF3R mutant clones, increases sensitivity, and earlier detection of CSF3R mutations acquired during the course of leukemogenic evolution of pre-leukemia HSCs of CN patients. We suggest application of sequencing of the entire CSF3R gene at diagnosis to identify patients with inherited lost-of-function CSF3R mutations and annual ultra-deep sequencing of the critical region of CSF3R to monitor acquisition of CSF3R mutations.

Published
2019

9. High Insta High Instability Of The CSF3R Gene In Severe Congenital Neutropenia Patients As Judged By Multiple CSF3R Mutations In The Majority Of Patients

Author

Klimiankou, Maksim, primary, Uenalan, Murat, additional, Kandabarau, Siarhei, additional, Wiehlmann, Lutz, additional, Hagemann, Anna-Lena, additional, Brechlin, Annette Müller, additional, Reuter, Marlene, additional, Zeidler, Cornelia, additional, Welte, Karl, additional, and Skokowa, Julia, additional

Published
2013
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10. Cooperativity Of RUNX1 and CSF3R Mutations In The Development Of Leukemia In Severe Congenital Neutropenia: A Unique Pathway In Myeloid Leukemogenesis

Author

Skokowa, Julia, primary, Steinemann, Doris, additional, Katsman-Kuipers, Jenny, additional, Zeidler, Cornelia, additional, Klimenkova, Olga, additional, Klimiankou, Maksim, additional, Uenalan, Murat, additional, Kandabarau, Siarhei, additional, Makaryan, Vahagn, additional, Beekman, Renee, additional, Stocking, Carol, additional, Obenauer, J, additional, Schnittger, Susanne, additional, Kohlmann, Alexander, additional, Valkhof, M, additional, Hoogenboezem, R, additional, Göhring, Gudrun, additional, Reinhardt, Dirk, additional, Schlegelberger, Brigitte, additional, Stanulla, Martin, additional, Vandenberghe, Peter, additional, Donadieu, Jean, additional, Zwaan, Michel, additional, Touw, Ivo P., additional, van den Heuvel-Eibrink, Marry M., additional, Dale, David C., additional, and Welte, Karl, additional

Published
2013
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13. Erratum: LEF-1 is crucial for neutrophil granulocytopoiesis and its expression is severely reduced in congenital neutropenia

Author

Skokowa, Julia, Cario, Gunnar, Uenalan, Murat, Schambach, Axel, Germeshausen, Manuela, Battmer, Karin, Zeidler, Cornelia, Lehmann, Ulrich, Eder, Matthias, Baum, Christopher, Grosschedl, Rudolf, Stanulla, Martin, Scherr, Michaela, and Welte, Karl

Abstract

Author(s): Julia Skokowa; Gunnar Cario; Murat Uenalan; Axel Schambach; Manuela Germeshausen; Karin Battmer; Cornelia Zeidler; Ulrich Lehmann; Matthias Eder; Christopher Baum; Rudolf Grosschedl; Martin Stanulla; Michaela Scherr; Karl Welte Nature [...]

Published
2006
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16. ACTH Production in C57BL/6 Mouse Skin

Author

SLOMINSKI, ANDRZEJ, primary, BOTCHKAREVA, NATALIA V., additional, BOTCHKAREV, VLADIMIR A., additional, CHAKRABORTY, ASHOK, additional, LUGER, THOMAS, additional, UENALAN, MURAT, additional, and PAUS, RALF, additional

Published
1999
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18. Cooperativity Of RUNX1and CSF3RMutations In The Development Of Leukemia In Severe Congenital Neutropenia: A Unique Pathway In Myeloid Leukemogenesis

Author

Skokowa, Julia, Steinemann, Doris, Katsman-Kuipers, Jenny, Zeidler, Cornelia, Klimenkova, Olga, Klimiankou, Maksim, Uenalan, Murat, Kandabarau, Siarhei, Makaryan, Vahagn, Beekman, Renee, Stocking, Carol, Obenauer, J, Schnittger, Susanne, Kohlmann, Alexander, Valkhof, M, Hoogenboezem, R, Göhring, Gudrun, Reinhardt, Dirk, Schlegelberger, Brigitte, Stanulla, Martin, Vandenberghe, Peter, Donadieu, Jean, Zwaan, Michel, Touw, Ivo P., van den Heuvel-Eibrink, Marry M., Dale, David C., and Welte, Karl

Published
2013
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20. Hair cycle control by estrogens: catagen induction via estrogen receptor (ER)-alpha is checked by ER beta signaling.

Author

Ohnemus U, Uenalan M, Conrad F, Handjiski B, Mecklenburg L, Nakamura M, Inzunza J, Gustafsson JA, and Paus R

Subjects
Animals, Apoptosis, Estradiol metabolism, Estradiol pharmacology, Estrogen Receptor beta genetics, Female, Fulvestrant, Hair physiology, Immunohistochemistry, Ligands, Mice, Mice, Inbred C57BL, Mice, Knockout, Models, Anatomic, Phenotype, RNA metabolism, RNA, Messenger metabolism, Reverse Transcriptase Polymerase Chain Reaction, Skin metabolism, Time Factors, Estradiol analogs & derivatives, Estrogen Receptor alpha metabolism, Estrogen Receptor beta metabolism, Estrogens metabolism, Hair metabolism
Abstract

Although 17beta-estradiol (E2) is recognized as a potent hair growth modulator, our knowledge of estrogen function, signaling, and target genes in hair biology is still very limited. Between the two recognized estrogen receptors (ERs), ER alpha and ER beta, only ER alpha had been detected in murine skin. Here we show that ER alpha, ER beta, and ER beta ins are all expressed throughout the murine hair cycle, both at the protein and RNA level, but show distinct expression patterns. We confirm that topical E2 arrests murine pelage hair follicles in telogen and demonstrate that E2 is a potent inducer of premature catagen development. The ER antagonist ICI 182.780 does not induce anagen prematurely but accelerates anagen development and wave spreading in female mice. ER beta knockout mice display accelerated catagen development along with an increase in the number of apoptotic hair follicle keratinocytes. This suggests that, contrary to previous concepts, ER beta does indeed play a significant role in murine hair growth control: whereas the catagen-promoting properties of E2 are mediated via ER alpha, ER beta mainly may function as a silencer of ER alpha action in hair biology. These findings illustrate the complexity of hair growth modulation by estrogens and suggest that one key to more effective hair growth manipulation with ER ligands lies in the use of selective ER alpha or -beta antagonists/agonists. Our study also underscores that the hair cycling response to estrogens offers an ideal model for studying the controls and dynamics of wave propagation in biological systems.

Published
2005
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