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279 results on '"Uemura, Tomohiro"'

14. The sorting of cargo proteins in the plant trans-Golgi network

Author

Shimizu, Yutaro and Uemura, Tomohiro

Subjects
Plant Science
Abstract

Membrane trafficking contributes to distinct protein compositions of organelles and is essential for proper organellar maintenance and functions. The trans-Golgi network (TGN) acts as a sorting station where various cargo proteins are sorted and directed to post-Golgi compartments, such as the multivesicular body or pre-vacuolar compartment, vacuoles, and plasma membrane. The spatial and temporal segregation of cargo proteins within the TGN, which is mediated with different sets of regulators including small GTPases and cargo adaptors, is a fundamental process in the sorting machinery. Recent studies with powerful imaging technologies have suggested that the TGN possesses spatially distinct subdomains or zones for different trafficking pathways. In this review, we will summarize the spatially and dynamically characteristic features of the plant TGN and their relation to cargo protein trafficking.

Published
2022

15. Trapa bispinosa Roxb. Pericarp Extract Exerts 5α-Reductase Inhibitory Activity in Castrated Benign Prostatic Hyperplasia Model Mice.

Author

Fujita, Takashi, Aoyama, Tomoko, Uemura, Tomohiro, Takeshita, Shouko, Yamasaki, Takuto, Heijou, Hiroko, and Morimoto, Koji

Subjects
BENIGN prostatic hyperplasia, PERICARP, LABORATORY mice, ANIMAL disease models, GALLIC acid
Abstract

Trapa bispinosa Roxb. pericarp extract (TBE) has a polyphenol-rich composition and exhibits potent antioxidant and anti-glycation activities in vitro. In the present study, we investigated the inhibitory effects of TBE on 5α-reductase in vitro using LNCaP cells and in vivo using a mouse model of castrated benign prostatic hyperplasia. TBE showed concentration-dependent inhibitory effects in the 5α-reductase (5αR) activity assay. In a reporter assay using AR-Luc/LNCaP cells, TBE inhibited the activity induced by testosterone, but not that induced by dihydrotestosterone. TBE also suppressed prostate cell proliferation, prostate-specific antigens, and transmembrane protease serine 2 expression in a castrated benign prostatic hyperplasia mouse model. In addition, ellagic acid, but not gallic acid, decreased 5αR and AR-Luc activities. Together, these results suggest a potential role for TBE in benign prostatic hyperplasia through inhibition of 5αR. [ABSTRACT FROM AUTHOR]

Published
2023
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22. Streptococcus oralis Meningitis with Gingival Bleeding in a Patient: A Case Report and Review of the Literature

Author

Nakamura, Yuki, Uemura, Tomohiro, Kawata, Yuka, Hirose, Bungo, Yamauchi, Rika, and Shimohama, Shun

Subjects
Aged, 80 and over, Male, Mouth, odontogenic bacteremia, meningitis, Case Report, Streptococcus oralis, Bacteremia, hematogenous transmission, Meningitis, Bacterial, gingival bleeding, poor oral hygiene, Streptococcal Infections, Humans
Abstract

An 81-year-old man with a history of gingival bleeding presented with a fever, headache, and drowsiness. His mouth and full dentures were unsanitary. Laboratory tests revealed Streptococcus oralis meningitis caused by odontogenic bacteremia. We reviewed eight reported cases, including the present case, because S. oralis meningitis is rare. Our review indicated that S. oralis meningitis needs to be considered when encountering cases of a fever, disturbance of consciousness, and headache with episodes of possible odontogenic bacteremia.

Published
2020

24. The TGN/EE SNARE protein SYP61 and the ubiquitin ligase ATL31 cooperatively regulate plant responses to carbon/nitrogen conditions in Arabidopsis

Author

Hasegawa, Yoko, primary, Huarancca Reyes, Thais, additional, Uemura, Tomohiro, additional, Baral, Anirban, additional, Fujimaki, Akari, additional, Luo, Yongming, additional, Morita, Yoshie, additional, Saeki, Yasushi, additional, Maekawa, Shugo, additional, Yasuda, Shigetaka, additional, Mukuta, Koki, additional, Fukao, Yoichiro, additional, Tanaka, Keiji, additional, Nakano, Akihiko, additional, Takagi, Junpei, additional, Bhalerao, Rishikesh P, additional, Yamaguchi, Junji, additional, and Sato, Takeo, additional

Published
2022
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35. Generation of cell polarity in plants links endocytosis, auxin distribution and cell fate decisions

Author

Dhonukshe, Pankaj, Tanaka, Hirokazu, Goh, Tatsuaki, Ebine, Kazuo, Mahonen, Ari Pekka, Prasad, Kalika, Blilou, Ikram, Geldner, Niko, Xu, Jian, Uemura, Tomohiro, Chory, Joanne, Ueda, Takashi, Nakano, Akihiko, Scheres, Ben, and Friml, Jiri

Subjects
Endocytosis -- Research -- Chemical properties, Membrane proteins -- Chemical properties -- Research, Cell interaction -- Research -- Chemical properties, Environmental issues, Science and technology, Zoology and wildlife conservation, Chemical properties, Research
Abstract

Dynamically polarized membrane proteins define different cell boundaries and have an important role in intercellular communication--a vital feature of multicellular development. Efflux carriers for the signalling molecule auxin from the [...]

Published
2008

36. TGN/EE SNARE protein SYP61 and the ubiquitin ligase ATL31 cooperatively regulate plant responses to carbon/nitrogen conditions in Arabidopsis.

Author

Hasegawa, Yoko, Reyes, Thais Huarancca, Uemura, Tomohiro, Baral, Anirban, Fujimaki, Akari, Luo, Yongming, Morita, Yoshie, Saeki, Yasushi, Maekawa, Shugo, Yasuda, Shigetaka, Mukuta, Koki, Fukao, Yoichiro, Tanaka, Keiji, Nakano, Akihiko, Takagi, Junpei, Bhalerao, Rishikesh P, Yamaguchi, Junji, and Sato, Takeo

Published
2022
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39. TGN/EE SNARE protein SYP61 is ubiquitinated and required for carbon/nitrogen-nutrient responses in Arabidopsis

Author

Hasegawa, Yoko, primary, Reyes, Thais Huarancca, additional, Uemura, Tomohiro, additional, Fujimaki, Akari, additional, Luo, Yongming, additional, Morita, Yoshie, additional, Saeki, Yasushi, additional, Baral, Anirban, additional, Maekawa, Shugo, additional, Yasuda, Shigetaka, additional, Mukuta, Koki, additional, Fukao, Yoichiro, additional, Tanaka, Keiji, additional, Nakano, Akihiko, additional, Bhalerao, Rishikesh P., additional, Sato, Takeo, additional, and Yamaguchi, Junji, additional

Published
2020
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44. A Golgi-Released Subpopulation of the Trans-Golgi Network Mediates Protein Secretion in Arabidopsis1[OPEN]

Author

Uemura, Tomohiro, Nakano, Ryohei Thomas, Takagi, Junpei, Wang, Yiming, Kramer, Katharina, Finkemeier, Iris, Nakagami, Hirofumi, Tsuda, Kenichi, Ueda, Takashi, Schulze-Lefert, Paul, and Nakano, Akihiko

Subjects
Arabidopsis Proteins, Cell Membrane, Arabidopsis, Golgi Apparatus, Articles, Plants, Genetically Modified, Enzymes, Plant Leaves, R-SNARE Proteins, Ascomycota, Cell Wall, Host-Pathogen Interactions, Mutation, biological phenomena, cell phenomena, and immunity, SNARE Proteins, Plant Diseases, trans-Golgi Network
Abstract

Spatiotemporal coordination of protein trafficking among organelles is essential for eukaryotic cells. The post-Golgi interface, including the trans-Golgi network (TGN), is a pivotal hub for multiple trafficking pathways. The Golgi-released independent TGN (GI-TGN) is a compartment described only in plant cells, and its cellular and physiological roles remain elusive. In Arabidopsis (Arabidopsis thaliana), the SYNTAXIN OF PLANTS (SYP) 4 group Qa-SNARE (soluble N-ethylmaleimide) membrane fusion proteins are shared components of TGN and GI-TGN and regulate secretory and vacuolar transport. Here we reveal that GI-TGNs mediate the transport of the R-SNARE VESICLE-ASSOCIATED MEMBRANE PROTEIN (VAMP) 721 to the plasma membrane. In interactions with a nonadapted powdery mildew pathogen, the SYP4 group of SNAREs is required for the dynamic relocation of VAMP721 to plant–fungus contact sites via GI-TGNs, thereby facilitating complex formation with its cognate SNARE partner PENETRATION1 to restrict pathogen entry. Furthermore, quantitative proteomic analysis of leaf apoplastic fluid revealed constitutive and pathogen-inducible secretion of cell wall-modification enzymes in a SYP4- and VAMP721-dependent manner. Hence, the GI-TGN acts as a transit compartment between the Golgi apparatus and the plasma membrane. We propose a model in which the GA-TGN matures into the GI-TGN and then into secretory vesicles by increasing the abundance of VAMP721-dependent secretory pathway components.

Published
2018

45. A Golgi-Released Subpopulation of the Trans-Golgi Network Mediates Protein Secretion in Arabidopsis

Author

Uemura, Tomohiro, Nakano, Ryohei Thomas, Takagi, Junpei, Wang, Yiming, Kramer, Katharina, Finkemeier, Iris, Nakagami, Hirofumi, Tsuda, Kenichi, Ueda, Takashi, Schulze-Lefert, Paul, Nakano, Akihiko, Uemura, Tomohiro, Nakano, Ryohei Thomas, Takagi, Junpei, Wang, Yiming, Kramer, Katharina, Finkemeier, Iris, Nakagami, Hirofumi, Tsuda, Kenichi, Ueda, Takashi, Schulze-Lefert, Paul, and Nakano, Akihiko

Abstract

Spatiotemporal coordination of protein trafficking among organelles is essential for eukaryotic cells. The post-Golgi interface, including the trans-Golgi network (TGN), is a pivotal hub for multiple trafficking pathways. The Golgi-released independent TGN (GI-TGN) is a compartment described only in plant cells, and its cellular and physiological roles remain elusive. In Arabidopsis (Arabidopsis thaliana), the SYNTAXIN OF PLANTS (SYP) 4 group Qa-SNARE (soluble N-ethylmaleimide) membrane fusion proteins are shared components of TGN and GI-TGN and regulate secretory and vacuolar transport. Here we reveal that GI-TGNs mediate the transport of the R-SNARE VESICLE-ASSOCIATED MEMBRANE PROTEIN (VAMP) 721 to the plasma membrane. In interactions with a nonadapted powdery mildew pathogen, the SYP4 group of SNAREs is required for the dynamic relocation of VAMP721 to plant-fungus contact sites via GI-TGNs, thereby facilitating complex formation with its cognate SNARE partner PENETRATION1 to restrict pathogen entry. Furthermore, quantitative proteomic analysis of leaf apoplastic fluid revealed constitutive and pathogen-inducible secretion of cell wall-modification enzymes in a SYP4- and VAMP721-dependent manner. Hence, the GI-TGN acts as a transit compartment between the Golgi apparatus and the plasma membrane. We propose a model in which the GA-TGN matures into the GI-TGN and then into secretory vesicles by increasing the abundance of VAMP721-dependent secretory pathway components.

Published
2019

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