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332 results on '"Udalova, Irina A."'

1. Single cell spatial analysis reveals inflammatory foci of immature neutrophil and CD8 T cells in COVID-19 lungs

Author

Weeratunga, Praveen, Denney, Laura, Bull, Joshua A., Repapi, Emmanouela, Sergeant, Martin, Etherington, Rachel, Vuppussetty, Chaitanya, Turner, Gareth D. H., Clelland, Colin, Woo, Jeongmin, Cross, Amy, Issa, Fadi, de Andrea, Carlos Eduardo, Melero Bermejo, Ignacio, Sims, David, McGowan, Simon, Zurke, Yasemin-Xiomara, Ahern, David J., Gamez, Eddie C., Whalley, Justin, Richards, Duncan, Klenerman, Paul, Monaco, Claudia, Udalova, Irina A., Dong, Tao, Antanaviciute, Agne, Ogg, Graham, Knight, Julian C., Byrne, Helen M., Taylor, Stephen, and Ho, Ling-Pei

Published
2023
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6. Constructing custom-made radiotranscriptomic signatures of vascular inflammation from routine CT angiograms: a prospective outcomes validation study in COVID-19

Author

Ahern, David J, Ai, Zhichao, Ainsworth, Mark, Allan, Chris, Allcock, Alice, Angus, Brian, Ansari, M Azim, Arancibia-Cárcamo, Carolina, Aschenbrenner, Dominik, Attar, Moustafa, Baillie, J Kenneth, Barnes, Eleanor, Bashford-Rogers, Rachael, Bashyal, Archana, Beer, Sally, Berridge, Georgina, Beveridge, Amy, Bibi, Sagida, Bicanic, Tihana, Blackwell, Luke, Bowness, Paul, Brent, Andrew, Brown, Andrew, Broxholme, John, Buck, David, Burnham, Katie, Byrne, Helen, Camara, Susana, Ferreira, Ivan Candido, Charles, Philip, Chen, Wentao, Chen, Yi-Ling, Chong, Amanda, Clutterbuck, Elizabeth, Coles, Mark, Conlon, Christopher, Cornall, Richard, Cribbs, Adam, Curion, Fabiola, Davenport, Emma, Davidson, Neil, Davis, Simon, Dendrou, Calliope, Dequaire, Julie, Dib, Lea, Docker, James, Dold, Christina, Dong, Tao, Downes, Damien, Drakesmith, Hal, Dunachie, Susanna, Duncan, David, Eijsbouts, Chris, Esnouf, Robert, Espinosa, Alexis, Etherington, Rachel, Fairfax, Benjamin, Fairhead, Rory, Fang, Hai, Fassih, Shayan, Felle, Sally, Fernandez Mendoza, Maria, Ferreira, Ricardo, Fischer, Roman, Foord, Thomas, Forrow, Aden, Frater, John, Fries, Anastasia, Gallardo Sanchez, Veronica, Garner, Lucy, Geeves, Clementine, Georgiou, Dominique, Godfrey, Leila, Golubchik, Tanya, Gomez Vazquez, Maria, Green, Angie, Harper, Hong, Harrington, Heather, Heilig, Raphael, Hester, Svenja, Hill, Jennifer, Hinds, Charles, Hird, Clare, Ho, Ling-Pei, Hoekzema, Renee, Hollis, Benjamin, Hughes, Jim, Hutton, Paula, Jackson-Wood, Matthew, Jainarayanan, Ashwin, James-Bott, Anna, Jansen, Kathrin, Jeffery, Katie, Jones, Elizabeth, Jostins, Luke, Kerr, Georgina, Kim, David, Klenerman, Paul, Knight, Julian, Kumar, Vinod, Kumar Sharma, Piyush, Kurupati, Prathiba, Kwok, Andrew, Lee, Angela, Linder, Aline, Lockett, Teresa, Lonie, Lorne, Lopopolo, Maria, Lukoseviciute, Martyna, Luo, Jian, Marinou, Spyridoula, Marsden, Brian, Martinez, Jose, Matthews, Philippa, Mazurczyk, Michalina, McGowan, Simon, McKechnie, Stuart, Mead, Adam, Mentzer, Alexander, Mi, Yuxin, Monaco, Claudia, Montadon, Ruddy, Napolitani, Giorgio, Nassiri, Isar, Novak, Alex, O'Brien, Darragh, O'Connor, Daniel, O'Donnell, Denise, Ogg, Graham, Overend, Lauren, Park, Inhye, Pavord, Ian, Peng, Yanchun, Penkava, Frank, Pereira Pinho, Mariana, Perez, Elena, Pollard, Andrew, Powrie, Fiona, Psaila, Bethan, Quan, T Phuong, Repapi, Emmanouela, Revale, Santiago, Silva-Reyes, Laura, Richard, Jean-Baptiste, Rich-Griffin, Charlotte, Ritter, Thomas, Rollier, Christine, Rowland, Matthew, Ruehle, Fabian, Salio, Mariolina, Sansom, Stephen Nicholas, Sanches Peres, Raphael, Santos Delgado, Alberto, Sauka-Spengler, Tatjana, Schwessinger, Ron, Scozzafava, Giuseppe, Screaton, Gavin, Seigal, Anna, Semple, Malcolm, Sergeant, Martin, Simoglou Karali, Christina, Sims, David, Skelly, Donal, Slawinski, Hubert, Sobrinodiaz, Alberto, Sousos, Nikolaos, Stafford, Lizzie, Stockdale, Lisa, Strickland, Marie, Sumray, Otto, Sun, Bo, Taylor, Chelsea, Taylor, Stephen, Taylor, Adan, Thongjuea, Supat, Thraves, Hannah, Todd, John, Tomic, Adriana, Tong, Orion, Trebes, Amy, Trzupek, Dominik, Tucci, Felicia Anna, Turtle, Lance, Udalova, Irina, Uhlig, Holm, van Grinsven, Erinke, Vendrell, Iolanda, Verheul, Marije, Voda, Alexandru, Wang, Guanlin, Wang, Lihui, Wang, Dapeng, Watkinson, Peter, Watson, Robert, Weinberger, Michael, Whalley, Justin, Witty, Lorna, Wray, Katherine, Xue, Luzheng, Yuen Yeung, Hing, Yin, Zixi, Young, Rebecca, Youngs, Jonathan, Zhang, Ping, Zurke, Yasemin-Xiomara, Banning, Adrian, Antonopoulos, Alexios, Bajaj, Amrita, Kelion, Andrew, Deshpande, Aparna, Kardos, Attila, Hudson, Benjamin, Koo, Bon-Kwon, Shirodaria, Cheerag, Xie, Cheng, Kotanidis, Christos, Mahon, Ciara, Berry, Colin, Adlam, David, Newby, David, Connolly, Derek, Scaletta, Diane, Alexander, Donna, Nicol, Ed, McAlindon, Elisa, Oikonomou, Evangelos, Pugliese, Francesca, Pontone, Gianluca, Benedetti, Giulia, He, Guo-Wei, West, Henry, Kondo, Hidekazu, Benedek, Imre, Das, Intrajeet, Deanfield, John, Graby, John, Greenwood, John, Rodrigues, Jonathan, Ge, Junbo, Channon, Keith, Fabritz, Larissa, Fan, Li-Juan, Kingham, Lucy, Guglielmo, Marco, Lyasheva, Maria, Schmitt, Matthias, Beer, Meinrad, Anderson, Michelle, Desai, Milind, Marwan, Mohamed, Takahashi, Naohiko, Mehta, Nehal, Dai, Neng, Screaton, Nicholas, Sabharwal, Nikant, Maurovich-Horvat, Pál, Rao, Praveen, Kotronias, Rafail, Kharbanda, Rajesh, Preston, Rebecca, Wood, Richard, Blankstein, Ron, Rajani, Ronak, Mirsadraee, Saeed, Munir, Shahzad, Thomas, Sheena, Neubauer, Stefan, Klömpken, Steffen, Petersen, Steffen, Achenbach, Stephan, Anthony, Susan, Mak, Sze, Mittal, Tarun, Benedek, Theodora, Sharma, Vinoda, Lin, Wen-Hua, Kotanidis, Christos P, Rodrigues, Jonathan C L, O’Connor, Daniel, Siddique, Muhammad, Lockstone, Helen, Oikonomou, Evangelos K, Badi, Ileana, Lumley, Sheila F, Constantinides, Bede, Sanderson, Nicholas, Rodger, Gillian, Chau, Kevin K, Lodge, Archie, Tsakok, Maria, Gleeson, Fergus, Indrajeet, Das, Hudson, Benjamin J, Srivastava, Vivek, Farid, Shakil, Krasopoulos, George, Sayeed, Rana, Newby, David E, Channon, Keith M, and Antoniades, Charalambos

Published
2022
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10. Digital transformations in education: Augmented reality in the classroom.

Author

Arkhipova, Maria V., Shutova, Natalia V., Orlova, Olga A., Zhernovaya, Oksana R., Zintsova, Yulia N., and Udalova, Irina M.

Subjects
DIGITAL transformation, EDUCATIONAL planning, DIGITAL technology, EDUCATIONAL technology, AUGMENTED reality, COMPUTER assisted language instruction, DIGITAL natives, EDUCATIONAL change
Abstract

Technology brings significant changes to education, giving teachers opportunities to introduce new educational methods and strategies and build immersive learning experiences important for achieving success in the digital world. Augmented reality is an innovative educational tool, presenting information in a new way that appeals to the modern generation of digital native learners. The article evaluates the impact of augmented reality technology on motivation to learn a foreign language by higher University students. Motivation is the key to successful language learning that stimulates learners to realize their academic potential. The experiment reveals that augmented reality motivates students to learn better, influences their engagement and performance, and boosts learning indicators. This new-age technology, meeting the needs and interests of present-day generation, has a positive impact of students' attention, emotions, self-assurance and belief in academic achievement, promotes a better teacher-student understanding, and therefore opens a new dimension in education. [ABSTRACT FROM AUTHOR]

Published
2024
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12. Distinct synovial tissue macrophage subsets regulate inflammation and remission in rheumatoid arthritis

Author

Alivernini, Stefano, MacDonald, Lucy, Elmesmari, Aziza, Finlay, Samuel, Tolusso, Barbara, Gigante, Maria Rita, Petricca, Luca, Di Mario, Clara, Bui, Laura, Perniola, Simone, Attar, Moustafa, Gessi, Marco, Fedele, Anna Laura, Chilaka, Sabarinadh, Somma, Domenico, Sansom, Stephen N., Filer, Andrew, McSharry, Charles, Millar, Neal L., Kirschner, Kristina, Nerviani, Alessandra, Lewis, Myles J., Pitzalis, Costantino, Clark, Andrew R., Ferraccioli, Gianfranco, Udalova, Irina, Buckley, Christopher D., Gremese, Elisa, McInnes, Iain B., Otto, Thomas D., and Kurowska-Stolarska, Mariola

Published
2020
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13. Macrophage Activation and Polarization: Nomenclature and Experimental Guidelines

Author

Murray, Peter J, Allen, Judith E, Biswas, Subhra K, Fisher, Edward A, Gilroy, Derek W, Goerdt, Sergij, Gordon, Siamon, Hamilton, John A, Ivashkiv, Lionel B, Lawrence, Toby, Locati, Massimo, Mantovani, Alberto, Martinez, Fernando O, Mege, Jean-Louis, Mosser, David M, Natoli, Gioacchino, Saeij, Jeroen P, Schultze, Joachim L, Shirey, Kari Ann, Sica, Antonio, Suttles, Jill, Udalova, Irina, van Ginderachter, Jo A, Vogel, Stefanie N, and Wynn, Thomas A

Subjects
Biomedical and Clinical Sciences, Immunology, Animals, Granulocyte-Macrophage Colony-Stimulating Factor, Guidelines as Topic, Humans, Macrophage Activation, Macrophage Colony-Stimulating Factor, Macrophages, Mice, Research, Terminology as Topic
Abstract

Description of macrophage activation is currently contentious and confusing. Like the biblical Tower of Babel, macrophage activation encompasses a panoply of descriptors used in different ways. The lack of consensus on how to define macrophage activation in experiments in vitro and in vivo impedes progress in multiple ways, including the fact that many researchers still consider there to be only two types of activated macrophages, often termed M1 and M2. Here, we describe a set of standards encompassing three principles-the source of macrophages, definition of the activators, and a consensus collection of markers to describe macrophage activation-with the goal of unifying experimental standards for diverse experimental scenarios. Collectively, we propose a common framework for macrophage-activation nomenclature.

Published
2014

14. Neutrophil nucleus: shaping the past and the future.

Author

Salafranca, Julia, Ko, Jacky Ka, Mukherjee, Ananda K, Fritzsche, Marco, van Grinsven, Erinke, and Udalova, Irina A

Subjects
NEUTROPHILS, MACHINE learning, NUCLEAR shapes, GENETIC transcription regulation, IMAGE analysis
Abstract

Neutrophils are innate immune cells that are key to protecting the host against infection and maintaining body homeostasis. However, if dysregulated, they can contribute to disease, such as in cancer or chronic autoinflammatory disorders. Recent studies have highlighted the heterogeneity in the neutrophil compartment and identified the presence of immature neutrophils and their precursors in these pathologies. Therefore, understanding neutrophil maturity and the mechanisms through which they contribute to disease is critical. Neutrophils were first characterized morphologically by Ehrlich in 1879 using microscopy, and since then, different technologies have been used to assess neutrophil maturity. The advances in the imaging field, including state-of-the-art microscopy and machine learning algorithms for image analysis, reinforce the use of neutrophil nuclear morphology as a fundamental marker of maturity, applicable for objective classification in clinical diagnostics. New emerging approaches, such as the capture of changes in chromatin topology, will provide mechanistic links between the nuclear shape, chromatin organization, and transcriptional regulation during neutrophil maturation. Microscopy was the first technology used to identify neutrophils, and with the integration of advanced technologies is likely to remain key in the future. [ABSTRACT FROM AUTHOR]

Published
2023
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16. Principles of dimer-specific gene regulation revealed by a comprehensive characterization of NF-κB family DNA binding

Author

Siggers, Trevor, Chang, Abraham B, Teixeira, Ana, Wong, Daniel, Williams, Kevin J, Ahmed, Bilal, Ragoussis, Jiannis, Udalova, Irina A, Smale, Stephen T, and Bulyk, Martha L

Subjects
Biochemistry and Cell Biology, Biological Sciences, Biotechnology, Genetics, Animals, Base Sequence, Binding Sites, Cluster Analysis, DNA, Databases, Genetic, Gene Expression Regulation, Humans, Macrophages, Mice, NF-kappa B, Protein Array Analysis, Protein Binding, Protein Multimerization, Immunology, Biochemistry and cell biology
Abstract

The unique DNA-binding properties of distinct NF-κB dimers influence the selective regulation of NF-κB target genes. To more thoroughly investigate these dimer-specific differences, we combined protein-binding microarrays and surface plasmon resonance to evaluate DNA sites recognized by eight different NF-κB dimers. We observed three distinct binding-specificity classes and clarified mechanisms by which dimers might regulate distinct sets of genes. We identified many new nontraditional NF-κB binding site (κB site) sequences and highlight the plasticity of NF-κB dimers in recognizing κB sites with a single consensus half-site. This study provides a database that can be used in efforts to identify NF-κB target sites and uncover gene regulatory circuitry.

Published
2012

17. Principles of dimer-specific gene regulation revealed by a comprehensive characterization of NF-κB family DNA binding.

Author

Siggers, Trevor, Chang, Abraham B, Teixeira, Ana, Wong, Daniel, Williams, Kevin J, Ahmed, Bilal, Ragoussis, Jiannis, Udalova, Irina A, Smale, Stephen T, and Bulyk, Martha L

Subjects
Macrophages, Animals, Humans, Mice, NF-kappa B, DNA, Protein Array Analysis, Cluster Analysis, Gene Expression Regulation, Binding Sites, Base Sequence, Protein Binding, Databases, Genetic, Protein Multimerization, Databases, Genetic, Immunology
Abstract

The unique DNA-binding properties of distinct NF-κB dimers influence the selective regulation of NF-κB target genes. To more thoroughly investigate these dimer-specific differences, we combined protein-binding microarrays and surface plasmon resonance to evaluate DNA sites recognized by eight different NF-κB dimers. We observed three distinct binding-specificity classes and clarified mechanisms by which dimers might regulate distinct sets of genes. We identified many new nontraditional NF-κB binding site (κB site) sequences and highlight the plasticity of NF-κB dimers in recognizing κB sites with a single consensus half-site. This study provides a database that can be used in efforts to identify NF-κB target sites and uncover gene regulatory circuitry.

Published
2011

23. Unbiased single cell spatial analysis localises inflammatory clusters of immature neutrophils-CD8 T cells to alveolar progenitor cells in fatal COVID-19 lungs

Author

Weeratunga, Praveen, Denney, Laura, Bull, Joshua A., Repapi, Emmanouela, Sergeant, Martin, Etherington, Rachel, Chaitanya Vuppussetty, Turner, Gareth D.H., Clelland, Colin, Cross, Amy, Issa, Fadi, De Andrea, Carlos Eduardo, Bermejo, Ignacio Melero, Sims, David, McGowan, Simon, Yasemin-Xiomara Zurke, Ahern, David J., Gamez, Eddie C, Whalley, Justin, Richards, Duncan, Klenerman, Paul, Monaca, Claudia, Udalova, Irina A., Dong, Tao, Ogg, Graham, Knight, Julian C., Byrne, Helen M., Taylor, Stephen, and Ling-Pei Ho

Subjects
Organising pnemonia, Spatial proteomics, Immune-structural interactions, Multi-Dimensional Viewer (MDV) software, Spatial analysis, Inflammatory network, Severe COVID-19 disease, COVID-19 lung disease, Diffuse alveolar damage, Spatial Omics Oxford Pipeline (SpOOx)
Abstract

Single cell spatial interrogation of the immune-structural interactions in COVID -19 lungs is challenging, mainly because of the marked cellular infiltrate and architecturally distorted microstructure. To address this, we developed a suite of mathematical tools to search for statistically significant co-locations amongst immune and structural cells identified using 37-plex imaging mass cytometry. This unbiased method revealed a cellular map interleaved with an inflammatory network of immature neutrophils, cytotoxic CD8 T cells, megakaryocytes and monocytes co-located with regenerating alveolar progenitors and endothelium. Of note, a highly active cluster of immature neutrophils and cytotoxic CD8 T cells, was found spatially linked with alveolar progenitor cells, and temporally with the diffuse alveolar damage stage. These findings provide new insights into how immune cells interact in the lungs of severe COVID-19 disease. We provide our pipeline Spatial Omics Oxford Pipeline (SpOOx)and visual-analytical tool, Multi-Dimensional Viewer (MDV) software, as a resource for spatial analysis.

Published
2022
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25. Plasma iron controls neutrophil production and function

Author

Frost, Joe N., primary, Wideman, Sarah K., additional, Preston, Alexandra E., additional, Teh, Megan R., additional, Ai, Zhichao, additional, Wang, Lihui, additional, Cross, Amy, additional, White, Natasha, additional, Yazicioglu, Yavuz, additional, Bonadonna, Michael, additional, Clarke, Alexander J., additional, Armitage, Andrew E., additional, Galy, Bruno, additional, Udalova, Irina A., additional, and Drakesmith, Hal, additional

Published
2022
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26. Constructing custom-made radiotranscriptomic signatures of vascular inflammation from routine CT angiograms: a prospective outcomes validation study in COVID-19

Author

Kotanidis, Christos P, primary, Xie, Cheng, additional, Alexander, Donna, additional, Rodrigues, Jonathan C L, additional, Burnham, Katie, additional, Mentzer, Alexander, additional, O’Connor, Daniel, additional, Knight, Julian, additional, Siddique, Muhammad, additional, Lockstone, Helen, additional, Thomas, Sheena, additional, Kotronias, Rafail, additional, Oikonomou, Evangelos K, additional, Badi, Ileana, additional, Lyasheva, Maria, additional, Shirodaria, Cheerag, additional, Lumley, Sheila F, additional, Constantinides, Bede, additional, Sanderson, Nicholas, additional, Rodger, Gillian, additional, Chau, Kevin K, additional, Lodge, Archie, additional, Tsakok, Maria, additional, Gleeson, Fergus, additional, Adlam, David, additional, Rao, Praveen, additional, Indrajeet, Das, additional, Deshpande, Aparna, additional, Bajaj, Amrita, additional, Hudson, Benjamin J, additional, Srivastava, Vivek, additional, Farid, Shakil, additional, Krasopoulos, George, additional, Sayeed, Rana, additional, Ho, Ling-Pei, additional, Neubauer, Stefan, additional, Newby, David E, additional, Channon, Keith M, additional, Deanfield, John, additional, Antoniades, Charalambos, additional, Ahern, David J, additional, Ai, Zhichao, additional, Ainsworth, Mark, additional, Allan, Chris, additional, Allcock, Alice, additional, Angus, Brian, additional, Ansari, M Azim, additional, Arancibia-Cárcamo, Carolina, additional, Aschenbrenner, Dominik, additional, Attar, Moustafa, additional, Baillie, J Kenneth, additional, Barnes, Eleanor, additional, Bashford-Rogers, Rachael, additional, Bashyal, Archana, additional, Beer, Sally, additional, Berridge, Georgina, additional, Beveridge, Amy, additional, Bibi, Sagida, additional, Bicanic, Tihana, additional, Blackwell, Luke, additional, Bowness, Paul, additional, Brent, Andrew, additional, Brown, Andrew, additional, Broxholme, John, additional, Buck, David, additional, Byrne, Helen, additional, Camara, Susana, additional, Ferreira, Ivan Candido, additional, Charles, Philip, additional, Chen, Wentao, additional, Chen, Yi-Ling, additional, Chong, Amanda, additional, Clutterbuck, Elizabeth, additional, Coles, Mark, additional, Conlon, Christopher, additional, Cornall, Richard, additional, Cribbs, Adam, additional, Curion, Fabiola, additional, Davenport, Emma, additional, Davidson, Neil, additional, Davis, Simon, additional, Dendrou, Calliope, additional, Dequaire, Julie, additional, Dib, Lea, additional, Docker, James, additional, Dold, Christina, additional, Dong, Tao, additional, Downes, Damien, additional, Drakesmith, Hal, additional, Dunachie, Susanna, additional, Duncan, David, additional, Eijsbouts, Chris, additional, Esnouf, Robert, additional, Espinosa, Alexis, additional, Etherington, Rachel, additional, Fairfax, Benjamin, additional, Fairhead, Rory, additional, Fang, Hai, additional, Fassih, Shayan, additional, Felle, Sally, additional, Fernandez Mendoza, Maria, additional, Ferreira, Ricardo, additional, Fischer, Roman, additional, Foord, Thomas, additional, Forrow, Aden, additional, Frater, John, additional, Fries, Anastasia, additional, Gallardo Sanchez, Veronica, additional, Garner, Lucy, additional, Geeves, Clementine, additional, Georgiou, Dominique, additional, Godfrey, Leila, additional, Golubchik, Tanya, additional, Gomez Vazquez, Maria, additional, Green, Angie, additional, Harper, Hong, additional, Harrington, Heather, additional, Heilig, Raphael, additional, Hester, Svenja, additional, Hill, Jennifer, additional, Hinds, Charles, additional, Hird, Clare, additional, Hoekzema, Renee, additional, Hollis, Benjamin, additional, Hughes, Jim, additional, Hutton, Paula, additional, Jackson-Wood, Matthew, additional, Jainarayanan, Ashwin, additional, James-Bott, Anna, additional, Jansen, Kathrin, additional, Jeffery, Katie, additional, Jones, Elizabeth, additional, Jostins, Luke, additional, Kerr, Georgina, additional, Kim, David, additional, Klenerman, Paul, additional, Kumar, Vinod, additional, Kumar Sharma, Piyush, additional, Kurupati, Prathiba, additional, Kwok, Andrew, additional, Lee, Angela, additional, Linder, Aline, additional, Lockett, Teresa, additional, Lonie, Lorne, additional, Lopopolo, Maria, additional, Lukoseviciute, Martyna, additional, Luo, Jian, additional, Marinou, Spyridoula, additional, Marsden, Brian, additional, Martinez, Jose, additional, Matthews, Philippa, additional, Mazurczyk, Michalina, additional, McGowan, Simon, additional, McKechnie, Stuart, additional, Mead, Adam, additional, Mi, Yuxin, additional, Monaco, Claudia, additional, Montadon, Ruddy, additional, Napolitani, Giorgio, additional, Nassiri, Isar, additional, Novak, Alex, additional, O'Brien, Darragh, additional, O'Connor, Daniel, additional, O'Donnell, Denise, additional, Ogg, Graham, additional, Overend, Lauren, additional, Park, Inhye, additional, Pavord, Ian, additional, Peng, Yanchun, additional, Penkava, Frank, additional, Pereira Pinho, Mariana, additional, Perez, Elena, additional, Pollard, Andrew, additional, Powrie, Fiona, additional, Psaila, Bethan, additional, Quan, T Phuong, additional, Repapi, Emmanouela, additional, Revale, Santiago, additional, Silva-Reyes, Laura, additional, Richard, Jean-Baptiste, additional, Rich-Griffin, Charlotte, additional, Ritter, Thomas, additional, Rollier, Christine, additional, Rowland, Matthew, additional, Ruehle, Fabian, additional, Salio, Mariolina, additional, Sansom, Stephen Nicholas, additional, Sanches Peres, Raphael, additional, Santos Delgado, Alberto, additional, Sauka-Spengler, Tatjana, additional, Schwessinger, Ron, additional, Scozzafava, Giuseppe, additional, Screaton, Gavin, additional, Seigal, Anna, additional, Semple, Malcolm, additional, Sergeant, Martin, additional, Simoglou Karali, Christina, additional, Sims, David, additional, Skelly, Donal, additional, Slawinski, Hubert, additional, Sobrinodiaz, Alberto, additional, Sousos, Nikolaos, additional, Stafford, Lizzie, additional, Stockdale, Lisa, additional, Strickland, Marie, additional, Sumray, Otto, additional, Sun, Bo, additional, Taylor, Chelsea, additional, Taylor, Stephen, additional, Taylor, Adan, additional, Thongjuea, Supat, additional, Thraves, Hannah, additional, Todd, John, additional, Tomic, Adriana, additional, Tong, Orion, additional, Trebes, Amy, additional, Trzupek, Dominik, additional, Tucci, Felicia Anna, additional, Turtle, Lance, additional, Udalova, Irina, additional, Uhlig, Holm, additional, van Grinsven, Erinke, additional, Vendrell, Iolanda, additional, Verheul, Marije, additional, Voda, Alexandru, additional, Wang, Guanlin, additional, Wang, Lihui, additional, Wang, Dapeng, additional, Watkinson, Peter, additional, Watson, Robert, additional, Weinberger, Michael, additional, Whalley, Justin, additional, Witty, Lorna, additional, Wray, Katherine, additional, Xue, Luzheng, additional, Yuen Yeung, Hing, additional, Yin, Zixi, additional, Young, Rebecca, additional, Youngs, Jonathan, additional, Zhang, Ping, additional, Zurke, Yasemin-Xiomara, additional, Banning, Adrian, additional, Antonopoulos, Alexios, additional, Kelion, Andrew, additional, Kardos, Attila, additional, Hudson, Benjamin, additional, Koo, Bon-Kwon, additional, Kotanidis, Christos, additional, Mahon, Ciara, additional, Berry, Colin, additional, Newby, David, additional, Connolly, Derek, additional, Scaletta, Diane, additional, Nicol, Ed, additional, McAlindon, Elisa, additional, Oikonomou, Evangelos, additional, Pugliese, Francesca, additional, Pontone, Gianluca, additional, Benedetti, Giulia, additional, He, Guo-Wei, additional, West, Henry, additional, Kondo, Hidekazu, additional, Benedek, Imre, additional, Das, Intrajeet, additional, Graby, John, additional, Greenwood, John, additional, Rodrigues, Jonathan, additional, Ge, Junbo, additional, Channon, Keith, additional, Fabritz, Larissa, additional, Fan, Li-Juan, additional, Kingham, Lucy, additional, Guglielmo, Marco, additional, Schmitt, Matthias, additional, Beer, Meinrad, additional, Anderson, Michelle, additional, Desai, Milind, additional, Marwan, Mohamed, additional, Takahashi, Naohiko, additional, Mehta, Nehal, additional, Dai, Neng, additional, Screaton, Nicholas, additional, Sabharwal, Nikant, additional, Maurovich-Horvat, Pál, additional, Kharbanda, Rajesh, additional, Preston, Rebecca, additional, Wood, Richard, additional, Blankstein, Ron, additional, Rajani, Ronak, additional, Mirsadraee, Saeed, additional, Munir, Shahzad, additional, Klömpken, Steffen, additional, Petersen, Steffen, additional, Achenbach, Stephan, additional, Anthony, Susan, additional, Mak, Sze, additional, Mittal, Tarun, additional, Benedek, Theodora, additional, Sharma, Vinoda, additional, and Lin, Wen-Hua, additional

Published
2022
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27. Anti-TNF Therapy

Author

Udalova, Irina, primary, Monaco, Claudia, additional, Nanchahal, Jagdeep, additional, and Feldmann, Marc, additional

Published
2017
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30. Neutrophil phenotypes and functions in cancer: A consensus statement

Author

Quail, Daniela F., primary, Amulic, Borko, additional, Aziz, Monowar, additional, Barnes, Betsy J., additional, Eruslanov, Evgeniy, additional, Fridlender, Zvi G., additional, Goodridge, Helen S., additional, Granot, Zvi, additional, Hidalgo, Andrés, additional, Huttenlocher, Anna, additional, Kaplan, Mariana J., additional, Malanchi, Ilaria, additional, Merghoub, Taha, additional, Meylan, Etienne, additional, Mittal, Vivek, additional, Pittet, Mikael J., additional, Rubio-Ponce, Andrea, additional, Udalova, Irina A., additional, van den Berg, Timo K., additional, Wagner, Denisa D., additional, Wang, Ping, additional, Zychlinsky, Arturo, additional, de Visser, Karin E., additional, Egeblad, Mikala, additional, and Kubes, Paul, additional

Published
2022
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31. Deuterated Arachidonic Acid Ameliorates Lipopolysaccharide-Induced Lung Damage in Mice

Author

Molchanova, Alla Y., primary, Rjabceva, Svetlana N., additional, Melik-Kasumov, Tigran B., additional, Pestov, Nikolay B., additional, Angelova, Plamena R., additional, Shmanai, Vadim V., additional, Sharko, Olga L., additional, Bekish, Andrei V., additional, James, Genevieve, additional, Park, Hui Gyu, additional, Udalova, Irina A., additional, Brenna, J. Thomas, additional, and Shchepinov, Mikhail S., additional

Published
2022
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32. Plasma iron controls neutrophil production and function

Author

Frost, Joe N, primary, Wideman, Sarah K, additional, Preston, Alexandra E, additional, Teh, Megan R, additional, Ai, Zhichao, additional, Wang, Lihui, additional, Cross, Amy, additional, White, Natasha, additional, Yazicioglu, Yavuz, additional, Bonadonna, Michael, additional, Clarke, Alexander, additional, Armitage, Andrew E, additional, Galy, Bruno, additional, Udalova, Irina A, additional, and Drakesmith, Hal, additional

Published
2022
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33. A blood atlas of COVID-19 defines hallmarks of disease severity and specificity

Author

Ahern, David J., primary, Ai, Zhichao, additional, Ainsworth, Mark, additional, Allan, Chris, additional, Allcock, Alice, additional, Angus, Brian, additional, Ansari, M. Azim, additional, Arancibia-Cárcamo, Carolina V., additional, Aschenbrenner, Dominik, additional, Attar, Moustafa, additional, Baillie, J. Kenneth, additional, Barnes, Eleanor, additional, Bashford-Rogers, Rachael, additional, Bashyal, Archana, additional, Beer, Sally, additional, Berridge, Georgina, additional, Beveridge, Amy, additional, Bibi, Sagida, additional, Bicanic, Tihana, additional, Blackwell, Luke, additional, Bowness, Paul, additional, Brent, Andrew, additional, Brown, Andrew, additional, Broxholme, John, additional, Buck, David, additional, Burnham, Katie L., additional, Byrne, Helen, additional, Camara, Susana, additional, Candido Ferreira, Ivan, additional, Charles, Philip, additional, Chen, Wentao, additional, Chen, Yi-Ling, additional, Chong, Amanda, additional, Clutterbuck, Elizabeth A., additional, Coles, Mark, additional, Conlon, Christopher P., additional, Cornall, Richard, additional, Cribbs, Adam P., additional, Curion, Fabiola, additional, Davenport, Emma E., additional, Davidson, Neil, additional, Davis, Simon, additional, Dendrou, Calliope A., additional, Dequaire, Julie, additional, Dib, Lea, additional, Docker, James, additional, Dold, Christina, additional, Dong, Tao, additional, Downes, Damien, additional, Drakesmith, Hal, additional, Dunachie, Susanna J., additional, Duncan, David A., additional, Eijsbouts, Chris, additional, Esnouf, Robert, additional, Espinosa, Alexis, additional, Etherington, Rachel, additional, Fairfax, Benjamin, additional, Fairhead, Rory, additional, Fang, Hai, additional, Fassih, Shayan, additional, Felle, Sally, additional, Fernandez Mendoza, Maria, additional, Ferreira, Ricardo, additional, Fischer, Roman, additional, Foord, Thomas, additional, Forrow, Aden, additional, Frater, John, additional, Fries, Anastasia, additional, Gallardo Sanchez, Veronica, additional, Garner, Lucy C., additional, Geeves, Clementine, additional, Georgiou, Dominique, additional, Godfrey, Leila, additional, Golubchik, Tanya, additional, Gomez Vazquez, Maria, additional, Green, Angie, additional, Harper, Hong, additional, Harrington, Heather A., additional, Heilig, Raphael, additional, Hester, Svenja, additional, Hill, Jennifer, additional, Hinds, Charles, additional, Hird, Clare, additional, Ho, Ling-Pei, additional, Hoekzema, Renee, additional, Hollis, Benjamin, additional, Hughes, Jim, additional, Hutton, Paula, additional, Jackson-Wood, Matthew A., additional, Jainarayanan, Ashwin, additional, James-Bott, Anna, additional, Jansen, Kathrin, additional, Jeffery, Katie, additional, Jones, Elizabeth, additional, Jostins, Luke, additional, Kerr, Georgina, additional, Kim, David, additional, Klenerman, Paul, additional, Knight, Julian C., additional, Kumar, Vinod, additional, Kumar Sharma, Piyush, additional, Kurupati, Prathiba, additional, Kwok, Andrew, additional, Lee, Angela, additional, Linder, Aline, additional, Lockett, Teresa, additional, Lonie, Lorne, additional, Lopopolo, Maria, additional, Lukoseviciute, Martyna, additional, Luo, Jian, additional, Marinou, Spyridoula, additional, Marsden, Brian, additional, Martinez, Jose, additional, Matthews, Philippa C., additional, Mazurczyk, Michalina, additional, McGowan, Simon, additional, McKechnie, Stuart, additional, Mead, Adam, additional, Mentzer, Alexander J., additional, Mi, Yuxin, additional, Monaco, Claudia, additional, Montadon, Ruddy, additional, Napolitani, Giorgio, additional, Nassiri, Isar, additional, Novak, Alex, additional, O'Brien, Darragh P., additional, O'Connor, Daniel, additional, O'Donnell, Denise, additional, Ogg, Graham, additional, Overend, Lauren, additional, Park, Inhye, additional, Pavord, Ian, additional, Peng, Yanchun, additional, Penkava, Frank, additional, Pereira Pinho, Mariana, additional, Perez, Elena, additional, Pollard, Andrew J., additional, Powrie, Fiona, additional, Psaila, Bethan, additional, Quan, T. Phuong, additional, Repapi, Emmanouela, additional, Revale, Santiago, additional, Silva-Reyes, Laura, additional, Richard, Jean-Baptiste, additional, Rich-Griffin, Charlotte, additional, Ritter, Thomas, additional, Rollier, Christine S., additional, Rowland, Matthew, additional, Ruehle, Fabian, additional, Salio, Mariolina, additional, Sansom, Stephen Nicholas, additional, Sanches Peres, Raphael, additional, Santos Delgado, Alberto, additional, Sauka-Spengler, Tatjana, additional, Schwessinger, Ron, additional, Scozzafava, Giuseppe, additional, Screaton, Gavin, additional, Seigal, Anna, additional, Semple, Malcolm G., additional, Sergeant, Martin, additional, Simoglou Karali, Christina, additional, Sims, David, additional, Skelly, Donal, additional, Slawinski, Hubert, additional, Sobrinodiaz, Alberto, additional, Sousos, Nikolaos, additional, Stafford, Lizzie, additional, Stockdale, Lisa, additional, Strickland, Marie, additional, Sumray, Otto, additional, Sun, Bo, additional, Taylor, Chelsea, additional, Taylor, Stephen, additional, Taylor, Adan, additional, Thongjuea, Supat, additional, Thraves, Hannah, additional, Todd, John A., additional, Tomic, Adriana, additional, Tong, Orion, additional, Trebes, Amy, additional, Trzupek, Dominik, additional, Tucci, Felicia Anna, additional, Turtle, Lance, additional, Udalova, Irina, additional, Uhlig, Holm, additional, van Grinsven, Erinke, additional, Vendrell, Iolanda, additional, Verheul, Marije, additional, Voda, Alexandru, additional, Wang, Guanlin, additional, Wang, Lihui, additional, Wang, Dapeng, additional, Watkinson, Peter, additional, Watson, Robert, additional, Weinberger, Michael, additional, Whalley, Justin, additional, Witty, Lorna, additional, Wray, Katherine, additional, Xue, Luzheng, additional, Yeung, Hing Yuen, additional, Yin, Zixi, additional, Young, Rebecca K., additional, Youngs, Jonathan, additional, Zhang, Ping, additional, and Zurke, Yasemin-Xiomara, additional

Published
2022
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35. Irf5 deficiency in macrophages promotes beneficial adipose tissue expansion and insulin sensitivity during obesity

Author

Dalmas, Elise, Toubal, Amine, Alzaid, Fawaz, Blazek, Katrina, Eames, Hayley L., Lebozec, Kristell, Pini, Maria, Hainault, Isabelle, Montastier, Emilie, Denis, Raphael G.P., Ancel, Patricia, Lacombe, Amelie, Ling, Yin, Allatif, Omran, Cruciani-Guglielmacci, Celine, Andre, Sebastien, Viguerie, Nathalie, Poitou, Christine, Stich, Vladimir, Torcivia, Alexandra, Foufelle, Fabienne, Luquet, Serge, Aron-Wisnewsky, Judith, Langin, Dominique, Clement, Karine, Udalova, Irina A., and Venteclef, Nicolas

Subjects
Obesity -- Genetic aspects -- Care and treatment -- Research, Adipose tissues -- Comparative analysis -- Physiological aspects -- Genetic aspects -- Research, Insulin -- Comparative analysis -- Physiological aspects -- Genetic aspects -- Research, Macrophages -- Comparative analysis -- Physiological aspects -- Genetic aspects -- Research, Transcription factors -- Physiological aspects -- Research, Biological sciences, Health
Abstract

Accumulation of visceral adipose tissue correlates with elevated inflammation and increased risk of metabolic diseases. However, little is known about the molecular mechanisms that control its pathological expansion. Transcription factor interferon regulatory factor 5 (IRF5) has been implicated in polarizing macrophages towards an inflammatory phenotype. Here we demonstrate that mice lacking Irf5, when placed on a high-fat diet, show no difference in the growth of their epididymal white adipose tissue (epiWAT) but they show expansion of their subcutaneous white adipose tissue, as compared to wild-type (WT) mice on the same diet. EpiWAT from Irf5-deficient mice is marked by accumulation of alternatively activated macrophages, higher collagen deposition that restricts adipocyte size, and enhanced insulin sensitivity compared to epiWAT from WT mice. In obese individuals, IRF5 expression is negatively associated with insulin sensitivity and collagen deposition in visceral adipose tissue. Genome-wide analysis of gene expression in adipose tissue macrophages highlights the transforming growth factor β1 (TGFB1) gene itself as a direct target of IRF5-mediated inhibition. This study uncovers a new function for IRF5 in controlling the relative mass of different adipose tissue depots and thus insulin sensitivity in obesity, and it suggests that inhibition of IRF5 may promote a healthy metabolic state during this condition., Obesity and its common comorbidities, including glucose intolerance and type 2 diabetes (T2D), are frequently associated with the accumulation of adipose tissue in the intra-abdominal region, (so-called visceral obesity) (1). [...]

Published
2015
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36. Interferon regulatory factor-5-dependent CD11c+ macrophages contribute to the formation of rupture–prone atherosclerotic plaques

Author

Edsfeldt, Andreas, primary, Swart, Maarten, additional, Singh, Pratibha, additional, Dib, Lea, additional, Sun, Jiangming, additional, Cole, Jennifer E, additional, Park, Inhye, additional, Al-Sharify, Dania, additional, Persson, Ana, additional, Nitulescu, Mihaela, additional, Borges, Patricia Das Neves, additional, Kassiteridi, Christina, additional, Goddard, Michael E, additional, Lee, Regent, additional, Volkov, Petr, additional, Orho-Melander, Marju, additional, Maegdefessel, Lars, additional, Nilsson, Jan, additional, Udalova, Irina, additional, Goncalves, Isabel, additional, and Monaco, Claudia, additional

Published
2022
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40. Hyperglycemia Induces Trained Immunity in Macrophages and Their Precursors and Promotes Atherosclerosis

Author

Edgar, Laurienne, primary, Akbar, Naveed, additional, Braithwaite, Adam T., additional, Krausgruber, Thomas, additional, Gallart-Ayala, Héctor, additional, Bailey, Jade, additional, Corbin, Alastair L., additional, Khoyratty, Tariq E., additional, Chai, Joshua T., additional, Alkhalil, Mohammad, additional, Rendeiro, André F., additional, Ziberna, Klemen, additional, Arya, Ritu, additional, Cahill, Thomas J., additional, Bock, Christoph, additional, Laurencikiene, Jurga, additional, Crabtree, Mark J., additional, Lemieux, Madeleine E., additional, Riksen, Niels P., additional, Netea, Mihai G., additional, Wheelock, Craig E., additional, Channon, Keith M., additional, Rydén, Mikael, additional, Udalova, Irina A., additional, Carnicer, Ricardo, additional, and Choudhury, Robin P., additional

Published
2021
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44. The Zinc Finger Protein Zbtb18 Represses Expression of Class I Phosphatidylinositol 3-Kinase Subunits and Inhibits Plasma Cell Differentiation

Author

Xie, Bin, primary, Khoyratty, Tariq E., additional, Abu-Shah, Enas, additional, F. Cespedes, Pablo, additional, MacLean, Andrew J., additional, Pirgova, Gabriela, additional, Hu, Zhiyuan, additional, Ahmed, Ahmed A., additional, Dustin, Michael L., additional, Udalova, Irina A., additional, and Arnon, Tal I., additional

Published
2021
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46. The role of transposable elements in the regulation of IFN-[lambda]1 gene expression

Author

Thomson, Scott J.P., Goh, Fui G., Banks, Helen, Krausgruber, Thomas, Kotenko, Sergei V., Foxwell, Brian M.J., and Udalova, Irina A.

Subjects
Promoters (Genetics) -- Research, Gene expression -- Research, Interferon -- Properties, Transposons -- Properties, Science and technology
Abstract

IFNs [lambda]1, [lambda]2, and [lambda]3, or type III IFNs, are recently identified cytokines distantly related to type I IFNs. Despite an early evolutionary divergence, the 2 types of IFNs display similar antiviral activities, and both are produced primarily in dendritic cells. Although virus induction of the type I IFN-[beta] gene had served as a paradigm of gene regulation, relatively little is known about the regulation of IFN-[lambda] gene expression. Studies of virus induction of IFN-[lambda]1 identified an essential role of IFN regulatory factors (IRF) 3 and 7, which bind to a regulatory DNA sequence near the start site of transcription. Here, we report that the proximal promoter region of the IFN-[lambda]1 regulatory region is not sufficient for maximal gene induction in response to bacterial LPS, and we identify an essential cluster of homotypic NF-[kappa]B binding sites. Remarkably, these sites, which bind efficiently to NF-[kappa]B and function independently of the IRF3/7 binding sites, originate as transposable elements of the Alu and LTR families. We also show that depletion of the NF-[kappa]B RelA protein significantly reduces the level of the IFN-[lambda]1 gene expression. We conclude that IFN-[lambda]1 gene expression requires NF-[kappa]B, and we propose a model for IFN-[lambda]1 gene regulation, in which IRF and NF-[kapppa]B activate gene expression independently via spatially separated promoter elements. These observations provide insights into the independent evolution of the IFN-[lambda]1 and IFN-[beta] promoters and directly implicate transposable elements in the regulation of the IFN-[lambda]1 gene by NF-[kappa]B. gene regulation | interferon | transcription enhancer | NF-[kappa]B | Alu repeats

Published
2009

47. Synovial single-cell heterogeneity, zonation and interactions: a patchwork of effectors in arthritis.

Author

Schonfeldova, Barbora, Zec, Kristina, and Udalova, Irina A

Subjects
ENDOTHELIAL cells, SYNOVIAL membranes, NEURONS, INFLAMMATION, CELLULAR signal transduction, RHEUMATOID arthritis, GENE expression profiling, ARTHRITIS
Abstract

Despite extensive research, there is still no treatment that would lead to remission in all patients with rheumatoid arthritis as our understanding of the affected site, the synovium, is still incomplete. Recently, single-cell technologies helped to decipher the cellular heterogeneity of the synovium; however, certain synovial cell populations, such as endothelial cells or peripheral neurons, remain to be profiled on a single-cell level. Furthermore, associations between certain cellular states and inflammation were found; whether these cells cause the inflammation remains to be answered. Similarly, cellular zonation and interactions between individual effectors in the synovium are yet to be fully determined. A deeper understanding of cell signalling and interactions in the synovium is crucial for a better design of therapeutics with the goal of complete remission in all patients. [ABSTRACT FROM AUTHOR]

Published
2022
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48. ENDOTHELIAL CELL-DERIVED EXTRACELLULAR VESICLES ELICIT NEUTROPHIL DEPLOYMENT FROM THE SPLEEN FOLLOWING ACUTE MYOCARDIAL INFARCTION

Author

Akbar, Naveed, primary, Braithwaite, Adam, additional, Corr, Emma, additional, Koelwyn, Graeme, additional, van Solingen, Coen, additional, Cochain, Clement, additional, Saliba, Antoine-Emmanuel, additional, Corbin, Alastair, additional, Pezzolla, Daniela, additional, Edgar, Laurienne, additional, Gunadasa-Rohling, Mala, additional, Banerjee, Abhirup, additional, Paget, Daan, additional, Lee, Charlotte, additional, Hogg, Eleanor, additional, Costin, Adam, additional, Dhaliwal, Raman, additional, Johnson, Errin, additional, Krausgruber, Thomas, additional, Riepsaame, Joey, additional, Melling, Genevieve, additional, Shanmuganathan, Mayooran, additional, Acute Myocardial Infarction Study, Oxford, additional, Bock, Christoph, additional, Carter, David, additional, Channon, Keith, additional, Riley, Paul, additional, Udalova, Irina, additional, Moore, Kathryn, additional, Anthony, Daniel, additional, and Choudhury, Robin, additional

Published
2020
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49. IRF5 promotes influenza-induced inflammatory responses in human iPSC-derived myeloid cells and murine models

Author

Forbester, Jessica L, Clement, Mathew, Wellington, Dannielle, Yeung, Amy, Dimonte, Sandra, Marsden, Morgan, Chapman, Lucy, Coomber, Eve L, Tolley, Charlotte, Lees, Emily, Hale, Christine, Clare, Simon, Udalova, Irina, Dong, Tao, Dougan, Gordon, Humphreys, Ian R, Tolley, Charlotte [0000-0002-1119-7983], Dougan, Gordon [0000-0003-0022-965X], and Apollo - University of Cambridge Repository

Subjects
Macrophages, Induced Pluripotent Stem Cells, iPSCs, Adaptive Immunity, Virus Replication, Immunity, Innate, Disease Models, Animal, Mice, Orthomyxoviridae Infections, inflammation, Influenza A virus, IRF5, Host-Pathogen Interactions, Influenza, Human, Interferon Regulatory Factors, Interferon Type I, Animals, Humans, dendritic cells, influenza, Lung
Abstract

Recognition of Influenza A virus (IAV) by the innate immune system triggers pathways that restrict viral replication, activates innate immune cells, and regulates adaptive immunity. However, excessive innate immune activation can exaggerate disease. The pathways promoting excessive activation are incompletely understood, with limited experimental models to investigate mechanisms driving influenza-induced inflammation in humans. Interferon regulatory factor (IRF5) is a transcription factor that plays important roles in induction of cytokines after viral sensing. In an in vivo model of IAV infection, IRF5 deficiency reduced IAV-driven immune pathology and associated inflammatory cytokine production, specifically reducing cytokine-producing myeloid cell populations in Irf5 -/- mice, but not impacting type 1 IFN production or virus replication. Using cytometry by time-of-flight (CyTOF), we identified that human lung IRF5 expression was highest in cells of the myeloid lineage. To investigate the role of IRF5 in mediating human inflammatory responses by myeloid cells to IAV, we employed human induced pluripotent stem cells (hIPSCs) with biallelic mutations in IRF5, demonstrating for the first time iPS-derived dendritic cells (iPS-DCs) with biallelic mutations can be used to investigate regulation of human virus-induced immune responses. Using this technology, we reveal that IRF5 deficiency in human DCs, or macrophages, corresponded with reduced virus-induced inflammatory cytokine production, with IRF5 acting downstream of TLR7 and, possibly, RIG-I after viral sensing. Thus, IRF5 acts as a regulator of myeloid cell inflammatory cytokine production during IAV infection in mice and humans, and drives immune-mediated viral pathogenesis independently of type 1 IFN and virus replication.ImportanceThe inflammatory response to Influenza A virus (IAV) participates in infection control but contributes to disease severity. After viral detection intracellular pathways are activated, initiating cytokine production, but these pathways are incompletely understood. We show that interferon regulatory factor 5 (IRF5) mediates IAV-induced inflammation and, in mice, drives pathology. This was independent of antiviral type 1 IFN and virus replication, implying that IRF5 could be specifically targeted to treat influenza-induced inflammation. We show for the first time that human iPSC technology can be exploited in genetic studies of virus-induced immune responses. Using this technology, we deleted IRF5 in human myeloid cells. These IRF5-deficient cells exhibited impaired influenza-induced cytokine production and revealed that IRF5 acts downstream of Toll-like receptor 7 and possibly retinoic acid-inducible gene-I. Our data demonstrate the importance of IRF5 in influenza-induced inflammation, suggesting genetic variation in the IRF5 gene may influence host susceptibility to viral diseases.

Published
2020

50. Воспоминания Надежды Моллер – ценный источник для исследования биографии Н.Я. Бичурина

Author

Udalova Irina Vitalevna

Subjects
воспоминания, Моллер, Бичурин, биографистика
Abstract

В статье рассмотрены воспоминания внучатой племянницы Н.Я. Бичурина писательницы Н.С. Моллер в качестве источника для изучения биографии ученого, его характера и мировоззрения, влияния на их формирование конкретных фактов и обстоятельств китайского, петербургского и других периодов его жизни. Эти воспоминания были использованы в научной литературе и в документально-художественных произведениях.

Published
2020

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