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Your search keyword '"Uchiyama, Hironobu"' showing total 210 results
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210 results on '"Uchiyama, Hironobu"'

1. Identification of the growth cone as a probe and driver of neuronal migration in the injured brain

Author

Nakajima, Chikako, Sawada, Masato, Umeda, Erika, Takagi, Yuma, Nakashima, Norihiko, Kuboyama, Kazuya, Kaneko, Naoko, Yamamoto, Satoaki, Nakamura, Haruno, Shimada, Naoki, Nakamura, Koichiro, Matsuno, Kumiko, Uesugi, Shoji, Vepřek, Nynke A., Küllmer, Florian, Nasufović, Veselin, Uchiyama, Hironobu, Nakada, Masaru, Otsuka, Yuji, Ito, Yasuyuki, Herranz-Pérez, Vicente, García-Verdugo, José Manuel, Ohno, Nobuhiko, Arndt, Hans-Dieter, Trauner, Dirk, Tabata, Yasuhiko, Igarashi, Michihiro, and Sawamoto, Kazunobu

Published
2024
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15. Identification of an antiviral component from the venom of the scorpion Liocheles australasiae using transcriptomic and mass spectrometric analyses

Author

10219735, Miyashita, Masahiro, Mitani, Naoya, Kitanaka, Atsushi, Yakio, Mao, Chen, Ming, Nishimoto, Sachiko, Uchiyama, Hironobu, Sue, Masayuki, Hotta, Hak, Nakagawa, Yoshiaki, Miyagawa, Hisashi, 10219735, Miyashita, Masahiro, Mitani, Naoya, Kitanaka, Atsushi, Yakio, Mao, Chen, Ming, Nishimoto, Sachiko, Uchiyama, Hironobu, Sue, Masayuki, Hotta, Hak, Nakagawa, Yoshiaki, and Miyagawa, Hisashi

Abstract

Scorpion venom contains a variety of biologically active peptides. Among them, neurotoxins are major components in the venom, but it also contains peptides that show antimicrobial activity. Previously, we identified three insecticidal peptides from the venom of the Liocheles australasiae scorpion, but activities and structures of other venom components remained unknown. In this study, we performed a transcriptome analysis of the venom gland of the scorpion L. australasiae to gain a comprehensive understanding of its venom components. The result shows that potassium channel toxin-like peptides were the most diverse, whereas only a limited number of sodium channel toxin-like peptides were observed. In addition to these neurotoxin-like peptides, many non-disulfide-bridged peptides were identified, suggesting that these components have some critical roles in the L. australasiae venom. In this study, we also isolated a component with antiviral activity against hepatitis C virus using a bioassay-guided fractionation approach. By integrating mass spectrometric and transcriptomic data, we successfully identified LaPLA₂-1 as an anti-HCV component. LaPLA₂-1 is a phospholipase A₂ having a heterodimeric structure that is N-glycosylated at the N-terminal region. Since the antiviral activity of LaPLA₂-1 was inhibited by a PLA₂ inhibitor, the enzymatic activity of LaPLA₂-1 is likely to be involved in its antiviral activity.

Published
2021

16. Comparative analysis of enteroendocrine cells and their hormones between mouse intestinal organoids and native tissues

Author

Ohki, Junko, Sakashita, Akihiko, Aihara, Eitaro, Inaba, Akihiko, Uchiyama, Hironobu, Matsumoto, Masahito, Ninomiya, Yuzo, Yamane, Takumi, Oishi, Yuichi, and Iwatsuki, Ken

Subjects
endocrine system, digestive, oral, and skin physiology, digestive system, hormones, hormone substitutes, and hormone antagonists
Abstract

Endocrine cells in the gastrointestinal tract secrete multiple hormones to maintain homeostasis in the body. In the present study, we generated intestinal organoids from the duodenum, jejunum, and ileum of Neurogenin 3 (Ngn3)-EGFP mice and examined how enteroendocrine cells (EECs) within organoid cultures resemble native epithelial cells in the gut. Transcriptome analysis of EGFP-positive cells from Ngn3-EGFP organoids showed gene expression pattern comparable to EECs in vivo. We also compared mRNAs of five major hormones, namely, ghrelin (Ghrl), cholecystokinin (Cck), Gip, secretin (Sct), and glucagon (Gcg) in organoids and small intestine along the longitudinal axis and found that expression patterns of these hormones in organoids were similar to those in native tissues. These findings suggest that an intestinal organoid culture system can be utilized as a suitable model to study enteroendocrine cell functions in vitro. Statistical analysis of genes preferentially expressed in Ngn3+ vs Ngn3- cells derived from intestinal organoids of Ngn3-EGFP mice.

Published
2020
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17. Mutations in a β-group of solute carrier gene are responsible for egg and eye coloration of thebrown egg 4(b-4) mutant in the silkworm,Bombyx mori

Author

Tomihara, Kenta, primary, Satta, Katsuya, additional, Matsuzaki, Shohei, additional, Yoshitake, Kazutoshi, additional, Yamamoto, Kimiko, additional, Uchiyama, Hironobu, additional, Yajima, Shunsuke, additional, Futahashi, Ryo, additional, Katsuma, Susumu, additional, Osanai-Futahashi, Mizuko, additional, and Kiuchi, Takashi, additional

Published
2021
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29. Reducing the Number of Long-term Items : Optimal Strategy for a Bag-packing Problem

Author

Murakami, Yoshihiro, Kurata, Junichi, Uchiyama, Hironobu, and Marquardt, Wolfgang

Abstract

When several items having different weights are packed into a bag, the sum weight of each packed bag needs to be constant. The problem at packing is how to determine the items to be included in a bag so that the sum weight should be closest to the desired weight. In this paper, the following two algorithms are shown; one is to solve as an optimization problem and the other is to solve as a constraint satisfaction problem. Comparing the two algorithms, their functional rolls are made clear. In order to reduce the number of items remaining in scale hoppers for a long time, a method is proposed in which each of the two algorithms can be turned over one after another. Simulation results seem to prove that the proposed method is preferable.

Published
2005

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