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Fas-Fas ligand (FasL)-dependent pathways exert a suppressive effect on inflammatory responses in immune-privileged organs. FasL expression in hepatic Kupffer cells (KC) has been implicated in hepatic immunoregulation. In this study, modulation of FasL expression of KC by endogenous gut-derived bacterial LPS and the role of reactive oxygen species (ROS) as potential mediators of FasL expression in KC were investigated. LPS stimulation of KC resulted in upstream ROS generation and, subsequently, increased FasL expression and consequent Jurkat cell (Fas-positive) apoptosis. The NADPH oxidase and xanthine oxidase enzymatic pathways appear to be major sources of this upstream ROS generation. Increased FasL expression was blocked by antioxidants and by enzymatic blocking of ROS generation. Exogenous administration of [H.sub.2][O.sub.2] stimulated KC FasL expression and subsequent Jurkat cell apoptosis. Intracellular endogenous ROS generation may therefore represent an important signal transduction pathway for FasL expression in KC. antioxidants; hepatic immunoregulation

Background/Objectives: Speech impairment during the initial phase of removable partial denture (RPD) treatment can prevent patient adaptation to RPDs. This quasi-experimental study aimed to investigate the influence of the covering area of major connectors located in the mandibular anterior region on the accuracy of speech production. Methods: Fifteen adults (12 men and 3 women aged 26–40 years) with healthy dentition were recruited. Four target syllables were used for the assessments: [ʃi], [ʧi], [çi], and [ki]. There were four speaking conditions: without a connector (control), wearing lingual bar (5 mm width), wearing lingual plates (12 mm width), and wearing connectors covering the occlusal surface of lower dentition (16 mm width; CO). The accuracy of speech production was evaluated by mean appearance ratio of correct labels (MARC) and incorrect labels (MARIC) of the phonetic segments which were calculated with a speech evaluation system (VoiceAnalyzer). Results: The MARC in the 3 target syllables [ʃi], [ʧi], and [ki] significantly decreased with CO. For the target syllable [ʃi], the MARICs of [ʧi], [ʤi], and [çi] with CO were significantly lower than that in the control. For the target syllable [ʧi], the MARIC of [ki] with CO was significantly higher than that in the control. For the target syllable [çi], the MARIC of [gi] with CO was significantly higher than that in the control. Conclusion: RPDs with major connectors covering the lower dentition could disturb speech production during the initial phase of RPD treatment. Prosthodontic treatment with such RPD designs requires further consideration regarding the information given to patients before starting the treatment. [ABSTRACT FROM AUTHOR]

Background/aims: Surgical resection is the most effective therapy for metastatic colorectal cancer to the liver. However, the selection criteria for patients who may benefit from partial hepatectomy are not fully defined. The aim of this study was to evaluate the usefulness of perioperative molecular detection of circulating cancer cells in predicting clinical outcome in patients with colorectal metastatic liver cancer., Methodology: Blood samples were obtained perioperatively from the portal vein, peripheral artery, and superior vena cava in 16 consecutive patients with colorectal liver metastases who have undergone partial hepatic resection. We analyzed circulating cancer cells using a carcinoembryonic antigen-specific nested reverse transcriptase-polymerase chain reaction., Results: Positive carcinoembryonic antigen-mRNA expression was detected in 7 (43.8%) of 16 patients. Six (85.7%) of those 7 patients had hematogenous rerecurrences during the 1- to 3-year follow-up period. None of 9 negative-patients showed re-recurrence (p < 0.01). Among the 9 of 16 patients receiving postoperative adjuvant chemotherapy, no clear effect was noted regarding re-recurrence in the positive carcinoembryonic antigen-mRNA patients., Conclusions: These results suggest that the molecular detection of circulating cancer cells at the time of surgery for colorectal liver metastases could be one measure of high-risk patients for hematogenous re-recurrence after partial hepatic resection.