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Background: Potassium bromate (KBrO3) has been reported to be toxic, adversely affecting many body tissues and organs. The aim of this study was to determine the blood coagulation effect of Parkia biglobosa (P. biglobosa) seed on potassium bromate induced coagulopathy., Methodology: P. biglobosa was extracted with soxhlet extractor with ethanol as the solvent. Twenty-four adult male Wistar rats were acclimatized under laboratory conditions and were randomly grouped into A, B, C and D. Group A was given distilled water orally. Animals in groups B, C and D were administered 100 mg/kg body weight of potassium bromate, but groups C and D were also treated with 100 and 200 mg/kg body weight of P. biglobosa respectively. Both potassium bromate and P. biglobosa were freshly prepared on daily basis and administered to rats by oral gavage for 28 days. At the end of the treatment period, blood samples were collected in sodium citrate bottles and were used for analysis of Prothrombin Time (PT), Activated Partial Thromboplastin Time (APTT), Thrombin Time (TT), fibrinogen and vitamin K levels using standard methods., Results: Administration of potassium bromate increased Prothrombin Time (PT) from 11.67±2.15 seconds (in control animals) to 19.53±2.83 seconds. Treatment with 100 and 200 mg/kg body weight of P. biglobosa seed extract neutralized this effect in a dose-dependent manner. Likewise, KBrO 3 was observed to have significantly elevated Activated Partial Thromboplastin Time (APTT) from 29.67±3.93 to 41.10±4.79 seconds and Thrombin Time (TT) from 15.36±2.06 to 25.43±2.83 seconds when compared with those in the control group. The result further showed that exposure of animals to KBrO3 significantly declined the levels of fibrinogen (from 4.05±0.72 to 2.59±0.30 g/dL) and vitamin K (from 3.18±0.73 to 1.84±0.18 ng/mL) when compared with the untreated animals. The effect of KBrO 3 on PT, APTT, TT, Fibrinogen and vitamin k were attenuated by P. biglobosa in a dose-dependent manner., Conclusion: The results of this investigation demonstrated that potassium bromate caused prolongation of PT, aPTT and TT and decreased levels of fibrinogen and vitamin K, but P. biglobosa treatment counteracted these effects. Thus, it is recommended that these results be investigated in clinical trials in human volunteers., Competing Interests: The Authors declare that no competing interest exists., (Copyright © 2023 by West African Journal of Medicine.)

Background: Homozygous sickle cell patients are prone to renal damage which can be on-going in and out of crises, therefore, there is a need to monitor renal status using glomerular filtration rate. Equations to estimate GFR are readily available. Cockcroft-Gault equation is widely used, while the MDRD formula is the currently accepted equation. The CKD-EPI equation is recently being recommended but has not been validated among HbSS patients. Therefore, we aim to compare estimated GFR using CKD-EPI versus MDRD and Cockcroft-Gault equations among HbSS patients., Material and Methods: This was a cross-sectional study of stable HbSS patients. Information on their age, sex, and weight was collected. Their venous blood samples were also obtained for plasma creatinine determination which was used to calculate estimated GFR using Cockcroft-Gault, MDRD and CKD-EPI equations. Student t-test, Pearson correlation, and Bland-Altman difference plots were performed. A p-value of < 0.05 was considered to be significant., Results: One hundred and twenty patients comprising 60 HbSS patients and 60 HbAA controls participated in the study. The HbSS patients had mean ± SD age of 26±6.7years, plasma creatinine 77 ± 17umol/L, eGFR: CG 93±31.6ml/min, MDRD 124 ± 34.8ml/min/1.73m2, CKD-EPI 122 ± 25.1ml/min/1.73m2 (pConclusion: CKD-EPI equation is best for individuals with GFR > 60ml/min/1.73m2. This study has shown that it correlates well with the currently acceptable MDRD equation, therefore, can be used to monitor the renal status of stable HbSS patients. CG gives poor correlation and bias with CKD-EPI. Further validation studies on CKD-EPI equation are needed in different patient populations.