The objective of this research paper is to establish a suitable reactive dyeing process for banana fiber and comparison between dyeing behaviors of banana fibers with cotton fibers. Ambon (Cavendish type) banana variety was selected for this research study. Data accumulation is done by quantitative research methodology and experimental research strategies for this investigation; 5% enzyme and 6% H2O2-, 2% Na2SiO3-, and 3% NaOH-treated banana fibers were dyed with reactive dye. Banana fibers were dyed with three standard colors (red, blue, and yellow) each with four different concentrations (0.25, 1%, 4%, and 6%) of reactive dye. Testing was conducted to assess the color properties between pretreated banana fiber, dyed banana fiber, and cotton fiber. Color measurement was performed by using a Datacolour 600 spectrophotometers. The ΔE* values were used to determine the degree of color deterioration. Results showed that pretreated fibers become brighter (whiteness) than the raw banana fibers. Reflectance curves of dyed banana fibers were found similar to cotton in all the experiments and confirming the dye absorption tendency is more similar to cotton. Further results indicate that the dyeing behavior of banana is similar to cotton. Therefore, cotton dyeing process can be applied for the banana fibers. Dyeing of banana fiber was carried out with a reactive type of dye, which provided better washing fastness properties than cotton fibers.