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2 results on '"U274"'

1. In vivo functional characterization of the SARS-Coronavirus 3a protein in Drosophila

Author

Wong, S.L. Alan, Chen, Yiwei, Chan, Chak Ming, Chan, C.S. Michael, Chan, Paul K.S., Chui, Y.L., Fung, Kwok Pui, Waye, Mary M.Y., Tsui, Stephen K.W., and Chan, H.Y. Edwin

Subjects
*DROSOPHILA, *MOLECULAR cloning, *APOPTOSIS, *MONOCLONAL antibodies
Abstract

Abstract: The Severe Acute Respiratory Syndrome-Coronavirus (SARS-CoV) 3a locus encodes a 274 a.a. novel protein, and its expression has been confirmed in SARS patients. To study functional roles of 3a, we established a transgenic fly model for the SARS-CoV 3a gene. Misexpression of 3a in Drosophila caused a dominant rough eye phenotype. Using a specific monoclonal antibody, we demonstrated that the 3a protein displayed a punctate cytoplasmic localization in Drosophila as in SARS-CoV-infected cells. We provide genetic evidence to support that 3a is functionally related to clathrin-mediated endocytosis. We further found that 3a misexpression induces apoptosis, which could be modulated by cellular cytochrome c levels and caspase activity. From a forward genetic screen, 78 dominant 3a modifying loci were recovered and the identity of these modifiers revealed that the severity of the 3a-induced rough eye phenotype depends on multiple cellular processes including gene transcriptional regulation. [Copyright &y& Elsevier]

Published
2005
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2. In vivo functional characterization of the SARS-Coronavirus 3a protein in Drosophila

Author

Chak Ming Chan, Yiwei Chen, Yiu-Loon Chui, C.S. Michael Chan, H.Y. Edwin Chan, Stephen Kwok-Wing Tsui, Mary M.Y. Waye, S.L. Alan Wong, Kwok-Pui Fung, and Paul K.S. Chan

Subjects
Cytoplasm, Transgene, Biophysics, Locus (genetics), Biology, Endocytosis, Genetic screen, Biochemistry, Article, Viroporin Proteins, Animals, Genetically Modified, Viral Proteins, Viral Envelope Proteins, Transcriptional regulation, Animals, Humans, U274, Molecular Biology, Gene, Cytochrome c, fungi, Gene Transfer Techniques, X1, Antibodies, Monoclonal, Cell Biology, Phenotype, Molecular biology, Clathrin, Severe acute respiratory syndrome-related coronavirus, biology.protein, Drosophila, Transgenics
Abstract

The Severe Acute Respiratory Syndrome-Coronavirus (SARS-CoV) 3a locus encodes a 274 a.a. novel protein, and its expression has been confirmed in SARS patients. To study functional roles of 3a, we established a transgenic fly model for the SARS-CoV 3a gene. Misexpression of 3a in Drosophila caused a dominant rough eye phenotype. Using a specific monoclonal antibody, we demonstrated that the 3a protein displayed a punctate cytoplasmic localization in Drosophila as in SARS-CoV-infected cells. We provide genetic evidence to support that 3a is functionally related to clathrin-mediated endocytosis. We further found that 3a misexpression induces apoptosis, which could be modulated by cellular cytochrome c levels and caspase activity. From a forward genetic screen, 78 dominant 3a modifying loci were recovered and the identity of these modifiers revealed that the severity of the 3a-induced rough eye phenotype depends on multiple cellular processes including gene transcriptional regulation.

Published
2005

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