Search

Tyromyces floriformis, a potent fungal sesquiterpene producer, was grown Cerrena unicolor, as a model organism in submerged culture to search for chemicals affecting sesquiterpene biosynthe...

Phylogenetic and taxonomic studies on the brown-rot fungi Postia and related genera, are carried out. Phylogenies of these fungi are reconstructed with multiple loci DNA sequences including the internal transcribed spacer regions (ITS), the large subunit (nLSU) and the small subunit (nSSU) of nuclear ribosomal RNA gene, the small subunit of mitochondrial rRNA gene (mtSSU), the translation elongation factor 1-α gene (TEF1), the largest subunit of RNA polymerase II (RPB1) and the second subunit of RNA polymerase II (RPB2). Ten distinct clades of Postia s.lat. are recognized. Four new genera, Amaropostia, Calcipostia, Cystidiopostia and Fuscopostia, are established, and nine new species, Amaropostia hainanensis, Cyanosporus fusiformis, C. microporus, C. mongolicus, C. piceicola, C. subhirsutus, C. tricolor, C. ungulatus and Postia sublowei, are identified. Illustrated descriptions of the new genera and species are presented. Identification keys to Postia and related genera, as well as keys to the species of each genus, are provided.

This study aimed to examine the antibacterial activity of extracts of Amazonian Agaricomycetes macrofungi. The sample comprised 168 specimen collected at the Zoobotanical Park of the Federal University of Acre (Brazil), of which 32 (19%) were isolated. Extracts of the 32 isolates and 28 Agaricomycetes basidiomata were prepared and tested for activity against Staphylococcus aureus, Streptococcus pneumoniae, Escherichia coli, and Klebsiella pneumoniae bacteria, using the disc diffusion method. Of the 32 metabolite extracts of the isolated Basidiomycetes, 11 (34.4%) exhibited antibacterial activity especially against Gram-negative E. coli and K. pneumoniae bacteria, while of the 28 basidiomata extracts, 6 (21.4%) exhibited antibacterial activity mainly against the Gram-positive S. aureus bacteria. This study is the first to report the antibacterial activity of the Agaricomycetes species Coriolopsis caperata, Cyclomyces iodinus, Cymatoderma sp., Favolus tenuiculus, and Tyromyces cf. polyporoides, and contributes as the first investigation about the antibacterial potential of Basidiomycetes grown in Southwest Amazonia.

Baluran National Park, West Java, as one of the conservation sites in Indonesia, has the attraction of the varied types of ecosystems, including fungi. This study aimed to analyze the diversity of fungi in Bama Coastal Forest, Baluran National Park. The method was explorative with plot purposive sampling technique. Parameters in this study include abundance, dominance, and diversity of fungi enriched with physical parameters of humidity and temperature. The fungi were documented and macroscopically observed. Data were analyzed using the abundance index, dominance index, and diversity index. This research identified 18 types of macrofungi in Bama Coastal forest, Baluran National Park East Java including Ganoderma, sp, Hexagonia tenuis, Trametes hirsute, Phellinus sp.1 and sp.2, Ganoderma applanatum, Phellinus igniarius, Pycnoporus cinnabarinus, Daedalea quercina, Tyromyces chioneus, Microporus xanthopus, Calvatia sp., Irpex lacteus, Trichaptum sp., Lentinus sp. Poria corticola, Tyromyces sp., and Lichemomphalia sp. One fungi species (Ganoderma sp.) has the highest abundance index (27.62). The Medium abundance index was found in three species (Hexagonia tenuis, Tyromyces chioneus, Tyromyces sp.) while the rest fungi were in low abundance index. Dominance index of all fungi species identified in Bama was in low category (D

Odoria (Meruliaceae, Basidiomycota) is described as a new genus established for the threatened old-growth forest polypore Phaeolus alborubescens that was previously discussed in Aurantiporus or Tyromyces. It is characterized by the annual pinkish white spongy basidiocarp with strong sweetish smell, turning pale brown when drying, and has a positive reaction with KOH solution, the lack of cystidia, and the monomitic hyphal system with clamped generative hyphae often covered with fine orange crystals. Multigene phylogenetic analyses based on a combined (5.8S–nucLSU–rpb1–rpb2) and a single locus (nrITS) dataset place Odoria in Meruliaceae (Phlebioid clade), close to Crustodontia, Sarcodontia, Luteoporia, and Phlebiporia. Molecular as well as anatomical study of the old type specimen of P. alborubescens is also provided.

This is the sixth in a series of papers where we bring collaborating mycologists together to produce a set of notes of several taxa of fungi. In this study we introduce a new family Fuscostagonosporaceae in Dothideomycetes. We also introduce the new ascomycete genera Acericola, Castellaniomyces, Dictyosporina and Longitudinalis and new species Acericola italica, Alternariaster trigonosporus, Amarenomyces dactylidis, Angustimassarina coryli, Astrocystis bambusicola, Castellaniomyces rosae, Chaetothyrina artocarpi, Chlamydotubeufia krabiensis, Colletotrichum lauri, Collodiscula chiangraiensis, Curvularia palmicola, Cytospora mali-sylvestris, Dictyocheirospora cheirospora, Dictyosporina ferruginea, Dothiora coronillae, Dothiora spartii, Dyfrolomyces phetchaburiensis, Epicoccum cedri, Epicoccum pruni, Fasciatispora calami, Fuscostagonospora cytisi, Grandibotrys hyalinus, Hermatomyces nabanheensis, Hongkongmyces thailandica, Hysterium rhizophorae, Jahnula guttulaspora, Kirschsteiniothelia rostrata, Koorchalomella salmonispora, Longitudinalis nabanheensis, Lophium zalerioides, Magnibotryascoma mali, Meliola clerodendri-infortunati, Microthyrium chinense, Neodidymelliopsis moricola, Neophaeocryptopus spartii, Nigrograna thymi, Ophiocordyceps cossidarum, Ophiocordyceps issidarum, Ophiosimulans plantaginis, Otidea pruinosa, Otidea stipitata, Paucispora kunmingense, Phaeoisaria microspora, Pleurothecium floriforme, Poaceascoma halophila, Periconia aquatica, Periconia submersa, Phaeosphaeria acaciae, Phaeopoacea muriformis, Pseudopithomyces kunmingnensis, Ramgea ozimecii, Sardiniella celtidis, Seimatosporium italicum, Setoseptoria scirpi, Torula gaodangensis and Vamsapriya breviconidiophora. We also provide an amended account of Rhytidhysteron to include apothecial ascomata and a J+ hymenium. The type species of Ascotrichella hawksworthii (Xylariales genera incertae sedis), Biciliopsis leptogiicola (Sordariomycetes genera incertae sedis), Brooksia tropicalis (Micropeltidaceae), Bryochiton monascus (Teratosphaeriaceae), Bryomyces scapaniae (Pseudoperisporiaceae), Buelliella minimula (Dothideomycetes genera incertae sedis), Carinispora nypae (Pseudoastrosphaeriellaceae), Cocciscia hammeri (Verrucariaceae), Endoxylina astroidea (Diatrypaceae), Exserohilum turcicum (Pleosporaceae), Immotthia hypoxylon (Roussoellaceae), Licopolia franciscana (Vizellaceae), Murispora rubicunda (Amniculicolaceae) and Doratospora guianensis (synonymized under Rizalia guianensis, Trichosphaeriaceae) were re-examined and descriptions, illustrations and discussion on their familial placement are given based on phylogeny and morphological data. New host records or new country reports are provided for Chlamydotubeufia huaikangplaensis, Colletotrichum fioriniae, Diaporthe subclavata, Diatrypella vulgaris, Immersidiscosia eucalypti, Leptoxyphium glochidion, Stemphylium vesicarium, Tetraploa yakushimensis and Xepicula leucotricha. Diaporthe baccae is synonymized under Diaporthe rhusicola. A reference specimen is provided for Periconia minutissima. Updated phylogenetic trees are provided for most families and genera. We introduce the new basidiomycete species Agaricus purpurlesquameus, Agaricus rufusfibrillosus, Lactifluus holophyllus, Lactifluus luteolamellatus, Lactifluus pseudohygrophoroides, Russula benwooii, Russula hypofragilis, Russula obscurozelleri, Russula parapallens, Russula phoenicea, Russula pseudopelargonia, Russula pseudotsugarum, Russula rhodocephala, Russula salishensis, Steccherinum amapaense, Tephrocybella constrictospora, Tyromyces amazonicus and Tyromyces angulatus and provide updated trees to the genera. We also introduce Mortierella formicae in Mortierellales, Mucoromycota and provide an updated phylogenetic tree. Fil: Hyde, Kevin D.. Mae Fah Luang University. Center Of Excellence In Fungal Research; Tailandia Fil: Norphanphoun, Chada. Mae Fah Luang University. Center Of Excellence In Fungal Research; Tailandia Fil: Abreu, Vanessa P.. Universidade Federal de Viçosa; Brasil Fil: Bazzicalupo, Anna. University of British Columbia; Canadá Fil: Thilini Chethana, K. W.. Mae Fah Luang University. Center Of Excellence In Fungal Research; Tailandia Fil: Romero, Andrea Irene. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina Fil: Liu, Jian-Kui. Guizhou Academy of Agricultural Sciences; China Fil: Liu, Zuo-Yi. Guizhou Academy of Agricultural Sciences; China Fil: Longoni Plautz Jr., Helio. Instituto de Ciências Biológicas; Brasil Fil: Lumyong, Saisamorn. Chiang Mai University; Tailandia Fil: Maharachchikumbura, Sajeewa S. N.. Sultan Qaboos University; Omán Fil: Matočec, Neven. Ruđer Bošković Institute; Croacia Fil: McKenzie, Eric H. C.. Landcare Research Manaaki Whenua; Nueva Zelanda Fil: Mešić, Armin. Ruđer Bošković Institute; Croacia Fil: Miller, Daniel. Puget Sound Mycological Society; Estados Unidos Fil: Pawłowska, Julia. University of Warsaw; Polonia Fil: Pereira, Olinto L.. Universidade Federal de Viçosa; Brasil Fil: Promputtha, Itthayakorn. Mae Fah Luang University; Tailandia Fil: Ryvarden, Leif. University of Oslo; Noruega Fil: Su, Hong-Yan. Dali University; China Fil: Suetrong, Satinee. National Center for Genetic Engineering and Biotechnology; Tailandia Fil: Tkalčec, Zdenko. Ruđer Bošković Institute; Croacia Fil: Vizzini, Alfredo. Università di Torino; Italia Fil: Wen,Ting-Chi. Guizhou University; China Fil: Wisitrassameewong, Komsit. Seoul National University; Corea del Sur Fil: Wrzosek, Marta. University of Warsaw; Polonia Fil: Xu, Jian-Chu. Mae Fah Luang University; Tailandia Fil: Zhao, Qi. Chinese Academy of Sciences; República de China Fil: Zhao, Rui-Lin. Chinese Academy of Sciences; República de China Fil: Mortimer, Peter E.. Chinese Academy of Sciences; República de China

Oil palm decanter cake (OPDC) in the current study was converted to valuable products as laccase and manganese peroxidase (MnP) by an undescribed strain of the white-rot fungus, Pseudolagarobasidium sp. PP17-33. The optimization to enhance the production of enzymes through solid-state fermentation was performed using Plackett–Burman design and response surface methodology. The highest observed laccase was 5.841 U/gds and observed MnP was 5.156 U/gds, which enhanced yield by 2.59-fold and 1.94-fold from the non-optimization. The optimized medium (mg/g of OPDC) consisted of 0.852 mg CuSO4·5H2O, 13.512 mg glucose, 2 mg yeast extract, 0.2 mg KH2PO4, 1.5 mg MgSO4·7H2O, 0.01 mg FeSO4·7H2O, 0.15 mg MnSO4·H2O, 0.01 mg ZnSO4·7H2O and 0.3 mg Tween 80 (pH 5.0) when incubated at 30 °C for 7 days. The most significant variables of laccase and MnP productions were CuSO4·5H2O and glucose concentrations. This study is the first to report on the production of ligninolytic enzymes from OPDC waste using white-rot fungi. In addition, five different white-rot fungi, Coriolopsis aspera, C. retropicta, Dentipellis parmastoi, Nigroporus vinosus and Tyromyces xuchilensis, are newly observed producers of ligninolytic enzymes in Thailand. The results obtained from this study are significant not only for agro-industrial waste management but also for value-added enzyme production.

The fungal inhibitory effect of wood blocks with wood vinegar was investigated. Antifungal effect of wood vinegar and the anatomical aspect of pine wood against wood rot fungus were analysed and correlated. Although the effectiveness of wood vinegar as antibacterial and antifungal agent has been proven in food and agriculture field, no information is available concerning the preservative action against wood degrading fungi regarding the wooden building preservation. Wood vinegar (methylenechloride extract) was evaluated as wood preservative by soil block bioassay test (ASTM D1413-99). Softwood (Pinus densiflora) and hardwood (Zelkova serrata) blocks treated with five different concentrations of wood vinegar were subjected to wood decaying fungi, white rot fungi (Trametes versicolor) and brown rot fungi (Tyromyces palustris). Results revealed that 0.30 g/mL concentration of wood vinegar carried protection effect to the wood against white and brown rot. Wood vinegar showed an inhibitory effect on both growth and penetration of mycelia, thus defending the wooden tissue from destruction by prohibiting the erosion and thinning of cell walls. The protecting effect of fungal attack was determined by the extent of weight loss.

The new genus, Aegis Gómez-Montoya, Rajchenb. & Robledo, is described to accommodate the new species Aegis boa based on morphological data and phylogenetic evidences (ITS ? LSU rDNA). It is characterized by a particular monomitic hyphal system with thick-walled, widening, inflated and constricted generative hyphae, and allantoid basidiospores. Phylogenetically Aegis is closely related to Antrodiella aurantilaeta, both species presenting an isolated position within Polyporales into Grifola clade. The new taxon is so far known from Yungas Mountain Rainforests of NW Argentina. Fil: Gomez Montoya, Nataly. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina Fil: Rajchenberg, Mario. Centro de Investigación y Extensión Forestal Andino Patagónico; Argentina Fil: Robledo, Gerardo Lucio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina

In recent years, tannase has gained increasing interest mainly because of its potential applications. One of the most important functions of tannic acid (TA) hydrolase is the release of gallic acid (GA) from complex tannins. The aim of the study was to determine the dynamic changes in tannase activity depending on the carbon source in the culture medium. An extracellular and intracellular tannase activity analysis was carried out with the use of spectrophotometric analysis and confirmed by capillary electrophoresis in cultures of white-rot fungi: Phellinus pini, Fomes fomentarius, and Tyromyces pubescens. The inducible potential of TA and rapeseed meal on the activity of tannin acyl hydrolase was confirmed during 14 days of culturing. Different effects of the tested compounds on stimulation of tannase activity in selected fungal strains have been demonstrated. We concluded that rapeseed meal was the best inducer of tannase activity in the case of P. pini. However, the highest concentrations of GA were observed after stimulation by the TA in the cultures of F. fomentarius and T. pubescens.

The secretion of exopolysaccharides and oxalic acid in cultures of a white rot Ganoderma applanatum strain and a brown rot Tyromyces palustris strain were tested in terms of culture time, pH range, and temperature. The high yield of exopolysaccharides (EPS) required a moderate temperature of 28 °C for G. applanatum and 20 °C for T. palustris. G. applanatum and T. palustris accumulated more EPS when the concentration of the carbon source (maltose for G. applanatum and fructose for T. palustris) was 30 g/L. The results indicate that the production of oxalic acid by G. applanatum is correlated with the initial pH value of the culture medium and the concentration of oxalic acid increased to 1.66 ± 0.2 mM at the initial pH of 6.5 during the fungal growth. During the growth of T. palustris, the reduction of the initial pH value of the growing medium lowered the oxalic acid concentration from 7.7 ± 0.6 mM at pH 6.0 to 1.99 ± 0.2 mM at pH 3.5. T. palustris accumulated considerably more oxalic acid than G. applanatum and its presence did not affect significantly the production of exopolysaccharides. We also observed that the maximum amounts of exopolysaccharides secreted during cultivation of G. applanatum and T. palustris were 45.8 ± 1.2 and 19.1 ± 1.2 g/L, respectively.

We present a phylogenetic and phylogenomic overview of the Polyporales. The newly sequenced genomes of Bjerkandera adusta, Ganoderma sp., and Phlebia brevispora are introduced and an overview of 10 currently available Polyporales genomes is provided. The new genomes are 39 500 000-49 900 00 bp and encode for 12 910-16 170 genes. We searched available genomes for single-copy genes and performed phylogenetic informativeness analyses to evaluate their potential for phylogenetic systematics of the Polyporales. Phylogenomic datasets (25, 71, 356 genes) were assembled for the 10 Polyporales species with genome data and compared with the most comprehensive dataset of Polyporales to date (six-gene dataset for 373 taxa, including taxa with missing data). Maximum likelihood and Bayesian phylogenetic analyses of genomic datasets yielded identical topologies, and the corresponding clades also were recovered in the 373-taxa dataset although with different support values in some datasets. Three previously recognized lineages of Polyporales, antrodia, core polyporoid and phlebioid clades, are supported in most datasets, while the status of the residual polyporoid clade remains uncertain and certain taxa (e.g. Gelatoporia, Grifola, Tyromyces) apparently do not belong to any of the major lineages of Polyporales. The most promising candidate single-copy genes are presented, and nodes in the Polyporales phylogeny critical for the suprageneric taxonomy of the order are identified and discussed.

The chemical and morphological changes in heartwood specimens of Liquidambar orientalis Mill. caused by the white-rot fungus Trametes versicolor and the brown-rot fungi Tyromyces palustris and Gloeophyllum trabeum were studied by wet chemistry, FT-IR, GC-MS analyses, and photo-microscopy. According to GC-MS results, 26 extracts identified in the ethanol/toluene extraction and 17 in the ethanol extraction were found. Heartwood specimens of L. orientalis were highly susceptible to the fungi tested. While 1% NaOH solubility increased 35% in the specimen decayed by T. palustris, only an 8% increase was seen in the specimen exposed to T. versicolor when compared to the control specimen. Decayed wood by T. palustris showed a 5.5% increase in the Klason lignin content when compared to control specimens; however, the Klason lignin content decreased after a T. versicolor attack for 12 weeks. A T. versicolor attack in the cell walls was seen both from the lumina and from the cell corners, and the attack from the cell corners was mainly clear in ray parenchyma cells. An excessive destruction was detected in the wood structure attacked by T. palustris. The cell collapse was caused by a distortion in the plane of the wood cells. This extensive degradation was seen in all types of cell walls. Cracks in the cell walls were also detected in the specimens.

Subterranean termites need to minimize potentially pathogenic and competitive fungi in their environment in order to maintain colony health. We examined the ability of Actinobacteria isolated from termite guts in suppressing microorganisms commonly encountered in a subterranean environment. Guts from two subterranean termite species, Reticulitermes flavipes (Kollar) and Reticulitermes tibialis Banks, were extracted and plated on selective chitin media. A total of 38 Actinobacteria isolates were selected for in vitro growth inhibition assays. Target microbes included three strains of Serratia marcescens Bizio, two mold fungi (Trichoderma sp. and Metarhizium sp.), a yeast fungus (Candida albicans (C.P. Robin) Berkhout), and four basidiomycete fungi (Gloeophyllum trabeum (Persoon) Murrill, Tyromyces palustris (Berkeley & M.A. Curtis) Murrill, Irpex lacteus (Fries) Fries, and Trametes versicolor (L.) Lloyd). Results showed both broad and narrow ranges of antimicrobial activity against the mold fungi, yeast fungus, and S. marcescens isolates by the Actinobacteria selected. This suggests that termite gut-associated Actinobacteria produce secondary antimicrobial compounds that may be important for pathogen inhibition in termites. Basidiomycete fungi were strongly inhibited by the selected Actinobacteria isolates, with G. trabeum and T. versicolor being most inhibited, followed by I. lacteus and T. palustris. The degree of inhibition was correlated with shifts in pH caused by the Actinobacteria. Nearly all Actinobacteria isolates raised pH of the growth medium to basic levels (i.e. pH ∼8.0–9.5). We summarize antimicrobial activity of these termite gut-associated Actinobacteria and examine the implications of these pH shifts.

The genus Loweomyces is characterized by resupinate to pileate, sessile to stipitate basidiomes, monomitic to dimitic hyphal system with presence of pseudoskeletal hyphae, thin-walled hymenial cystidia, and usually cyanophilous spore and hyphal walls. The genus has not been widely accepted and some authors placed Loweomyces species in Abortiporus, Tyromyces or Antrodiella. During a survey of polypores in southern Brazil, two unidentified species with similar features to Loweomyces spp. were found. Morphological and molecular studies were carried out in order to understand their morphological and evolutionary relationships and to confirm their phylogenetic placement and taxonomic identity. DNA sequences of the internal transcribed spacer and large subunit of ribosomal RNA gene were used for phylogenetic studies. Both studied species have similar morphological characteristics to other Loweomyces spp. and group close to L. fractipes phylogenetically; therefore, Loweomyces spissus and L. tomentosus are proposed as new species. Full descriptions, illustrations of microscopic features, and an identification key to the accepted species in the genus are presented. Fil: Westphalen, Mauro C.. Instituto de Botanica de Sao Paulo; Brasil Fil: Tomšovský, Michal. Mendelova Univerzita V Brne; República Checa Fil: Rajchenberg, Mario. Centro de Investigación y Extensión Forestal Andino Patagónico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gugliotta, Adriana M.. Instituto de Botanica de Sao Paulo; Brasil

Herbs are widely used against the infections in public. The developing resistance of infection causing microorganisms to the antibiotics became a driving force for investigation of alternative antimicrobial agents. In this study, antimicrobial activity of ethanol and water extract of dried Solanum melongena fruit pericarp were determined against some gram positive, gram negative and some fungi. Solanum melongena is a common and popular plant worlwide and it has no side effect known. The investigation was accomplished by well diffusion method and the ethanol was more effective solvent for the extraction of active agents. The addition of NaCl (3%) improved antimicrobial activity slightly. Among the fungi tested, Tyromyces palustris was the only one sensitive to extracts. On the other hand, Enterobacter aerogenes and Pseudomonas aeruginosa showed the maximum sensitivity.

Spongipellis litschaueri, described in 1931 from Austria, is often synonymised with S. delectans in current literature. Morphological and molecular approaches were applied to revise the taxonomic position of this species. The basidiospore dimensions and DNA sequences confirmed differences between S. delectans and S. litschaueri, thus the latter should be kept as a distinct species. However, the relationship between S. litschaueri and its North American kin S. unicolor is complex. Although most of the examined specimens of S. unicolor differ significantly from S. litschaueri in morphological and molecular characters, one American isolate showed markedly high DNA sequence similarity to the European species. Moreover, specimens of S. unicolor from the more or less western USA have larger basidiospores than the others and the respective values are similar to S. litschaueri. Thereby, S. unicolor may split in two taxa where one is closely related to S. litschaueri. Additionally, DNA sequences of a large subunit of the nuclear ribosomal RNA gene were analysed to elucidate the position of Spongipellis within the Polyporales. The results revealed unexpected polyphyly when the generic type S. spumeus fell into a separate lineage from S. delectans, S. litschaueri, S. pachyodon, and S. unicolor. In addition, Tyromyces fissilis occasionally kept in Spongipellis is not related to any species of the genus nor to Tyromyces chioneus, the generic type of Tyromyces. An identification key of European Spongipellis species is provided.

Eleven strains of T. palustris were evaluated for mass loss and production of phosphate buffer soluble oxalic acid on pine wood blocks treated with 0.5% N′,N-naphthaloylhydroxamine (NHA) in a soil-block test. After 12 weeks higher percentage mass loss was observed in control groups for 10 strains, while TYP-6137 was shown to be tolerant with no difference between the untreated controls and 0.5% NHA-treated blocks. Strain TYP-6137 was evaluated at 6, 8, and 10 weeks for mass loss and production of oxalic acid in a colorimetric assay with increasing levels of NHA treatment (0.5–1%). Tyromyces palustris TYP-6137 was shown to be successful in degrading NHA-treated wood in the 0.5–0.8% treatment range, but was inhibited in the 0.9–1% treated groups. Although there is no simple linear correlation between mass loss and production of oxalic acid, there was an inverse relationship at the 6-week time period. Thus, it was concluded that production of oxalic acid in T. palustris TYP-6137 at 6 weeks may facilitate mass loss at 8–10 weeks in the 0.5–0.8% NHA-treated blocks. In the 0.9–1% treatment groups this response was not seen. The broad variation seen in oxalic acid production among the 11 T. palustris strains suggests that high oxalic acid production alone is not the sole mechanism of NHA tolerance. Oxalic acid appears to be non-specifically produced in response to the preservative treatment, yet its association with mass loss could not be clearly defined.

Two strains isolated from the organic layers of forests on Zijin Mountain have indicated a strong capability of decolorization for Orange G on the solid plates. They were identified as Phanerochaete sordida and Tyromyces lauteus according to phenotypic and molecular techniques. Through this study, we try to find the suitable condition and cheapest way for decolorization by two strains. The result shows that malt extract and ammonium sulfate are the best N source for P. sordida and T. lauteus, respectively; 0.95 g per L glucose + 0.05 g per L ethonal are the best C source both for P. sordida and T. lauteus. Oxalate plays an important role as the organic acid chelator which can also enhance the decolorized capability of fungi.

Bjerkandera is a worldwide polypore genus characterized by the pileate grayish basidiomes, often becoming darker when dried, and monomitic hyphal system. Tyromyces atroalbus is a rare Brazilian species re-discovered in recent surveys in Sao Paulo State. Even though it has been placed in Tyromyces, it presents a characteristic darkening of basidiomes when touched or dried, a feature absent in other species of the genus. In order to understand the evolutionary relations of T. atroalbus with Bjerkandera and Tyromyces and find its proper phylogenetic placement, morphological and molecular studies were carried out. Specimens from Brazil and Mexico were studied morphologically and selected specimens were used for DNA sequence analyses of internal transcribed spacer (ITS) and large subunit (LSU) of ribosomal RNA genes and translation elongation factor 1-alpha genes. Type specimens of T. atroalbus and T. humeana were also morphologically examined to confirm their possible synonymy as well as to confirm the identity of the recent collections. Molecular and morphological characters of T. atroalbus place the species in Bjerkandera. Our results also revealed that the specimens from Brazil and Mexico represent two distinct species. Therefore, the new combination Bjerkandera atroalba and description of new species B. centroamericana is published. In addition, the type study of T. atroalbus and T. humeana supports their synonymy.

The effect of menadione (MQ; 2-methyl-1,4-naphtoquinone) superoxide generating agent on the biological activity of two strains of white-rot fungi, Fomes fomentarius and Tyromyces pubescens, was determined. In this study 1 mM of MQ solution was added to 10-day-old idiophasic cultures. The application of MQ to F. fomentarius and T. pubescens cultures stimulated extracellular laccase (LAC) and manganese-dependent peroxidase (MnP) activities in comparison to the control values (without MQ). In the presence of MQ the concentration of oxalic acid in the medium of both fungi was dramatically decreased. MQ treatment also caused an increase of intracellular superoxide dismutase activity, formaldehyde and glutathione disulfide level in both strains. In the case of F. fomentarius, addition of MQ enhanced catalase activity. The rate of intra- and extracellular proteolysis decreased in F. fomentarius and increased in T. pubescens MQ treated cultures.

Antifungal activity of coconut shell pyrolytic oil against wood decay fungi was explored. Poisoned food technique was employed to indicate the antifungal effect of coconut shell pyrolytic oil against wood decay fungi. The results indicate that there was 81·5% inhibition on the growth of Polyporus sanguineus (L.)G. Mey, (white rot) and 90% inhibition on the growth of Tyromyces palustris (Berk M & A Curtis) (brown rot) at a concentration of 0·25%. This can be attributed to high phenolic content of coconut shell pyrolytic oil.

Wood still performs as the main materials for railway sleepers, especially at their connected portions, cross and bridge. So far, wood has not yet been effectively substituded by other materials. However, wood based railway sleepers are susceptible to deterioration by the decaying fungi. This study examined, the decay resistance of three wood species i.e. bangkirai (Shorea laevis), meranti batu (Shorea platyclados) and rasamala (Altingia exelsa). Samples were evaluated against the decayingfungi in a laboratory experiment using DIN 52176-modified standard. Results indicated that bangkirai and meranti batu were classified resistance against fungal attacks (Class II), whereas, rasamala was categorized moderately resistant (Class III). The weight loss percentage of rasamala was higher than those of bangkirai and meranti batu. The ability of fungus to degrade certain wood was depended upon kinds of wood and fungus itself. Fungi with significant decay ability were Pycnoporus sanguineus HHB-324, Schizophyllum commune, Polyporus sp., Trametes sp. and Tyromyces palustris. The highest weight losses were recorded on samples exposed consecutively to Pycnoporus sanguineus and Schizophyllum commune.

Four new triterpenoids, tyromycic acids B−E (1−4), were isolated from a methanol-soluble extract of the fruit bodies of the Japanese inedible mushroom Tyromyces fissilis. Tyromycic acids B−D posses...

Identifications were made by type examinations for 27 species of polypores described by Corner. Roseofavolus gen. nov. is proposed, typified by Grifola eos. The following new combinations are proposed: Amylocystis unicolor, Antrodiella xanthochroa, Cristelloporia pahangensis, C. rutilantiformis, Roseofavolus eos, Tyromyces armeniacus, T. olivascens, and Wrightoporia solomonensis. Hapalopilus rubescens is accepted in the original genus. The following species were already combined with the proper genus: Stecchericium gyroporum and S. trametoides. The following species are synonyms: Tyromyces sublamellatus, a synonym of Abortiporus roseus; T. subradiatus, a synonym of Daedaleopsis conchiformis; T. subroseiporus, a synonym of Fomitopsis scorteus; T. subtrimiticus, a synonym of Fomitopsis spraguei; T. sulphureiceps, a synonym of Antrodiella flava; and Loweporus corticicola, a synonym of C. rutilantiformis. The following species are dubious because of their poor or sterile conditions: Cristelloporia trimitica, Loweporus castaneus, Tyromyces subrubescens, and T. tristaniae. No authentic specimens were traced for Ischnoderma brunneipurpureum, I. friabile, I. solomonense, Loweporus ochraceicinctus, L. pileoliferus, Stecchericium trimiticum, and Tyromyces xanthophaes. Descriptions and line drawings are given for little-known species.

Antrodiella dentipora, Ceriporia albobrunnea, C. cystidiata, Diplomitoporus allantosporus, D. stramineus, D. venezuelicus, Flabellophora fasciculata, Navisporus perennis, Nigroporus macroporus, Polyporus albostipes, Rigidoporus aurantiacus, Skeletocutis microcarpa, Tinctoporellus isabellinus, Trametes olivaceopora, T. supermodesta, Trichaptum variabilis, Tyromyces neostrigosus, T. polyporoides and Wrightoporia roseocontexta are described as new. Keys to all, except Trametes, the new neotropical species in their respective genera are included. The combinations Trichaptum griseofuscens (Mont.) Ryvarden & Iturriaga, and Tyromyces americanus (D. Reid) Ryvarden & Iturriaga are proposed.

A total of 103 isolates of basidiomycetes, representing 84 species from different Brazilian ecosystems, were evaluated for their antifungal and antibacterial activity in a panel of pathogenic and non-pathogenic microorganisms. Tissue plugs of the fruiting bodies were cultivated in liquid media and the whole culture extracted with ethyl acetate. Crude extracts from Agaricus cf. nigrecentulus, Agrocybe perfecta, Climacodon pulcherrimus, Gloeoporus thelephoroides, Hexagonia hydnoides, Irpex lacteus, Leucoagaricus cf. cinereus, Marasmius cf. bellus, Marasmius sp., Nothopanus hygrophanus, Oudemansiella canarii, Pycnoporus sanguineus, Phellinus sp., and Tyromyces duracinus presented significant activity against one or more of the target microorganisms. Eight isolates were active only against bacteria while three inhibited exclusively the growth of fungi. Two extracts presented wide antimicrobial spectrum and were active against both fungi and bacteria. Differences in the bioactivity of extracts obtained from isolates from the same species were observed.

The increase in the use of non-arsenical copper-based wood preservatives in response to environmental concerns has been accompanied by interest in copper-tolerant decay fungi. Oxalic acid production by brown-rot fungi has been proposed as one mechanism of copper tolerance. Fifteen brown-rot fungi representing the genera Postia, Wolfiporia, Meruliporia, Gloeophyllum, Laetiporus, Coniophora, Antrodia, Serpula, and Tyromyces were evaluated for oxalic acid production bi-weekly in southern yellow pine (SYP) blocks treated with 1.2% ammoniacal copper citrate (CC). Eleven fungi were designated copper-tolerant based upon weight loss in CC-treated blocks. After 2 weeks, these fungi produced 2–17 times more oxalic acid in CC-treated blocks than in untreated blocks. After 10 weeks, weight loss ranged from 32% to 57% in CC-treated SYP. Four fungi were copper sensitive, producing low levels of oxalic acid and minimal weight loss in CC-treated blocks. Rapid induction of oxalic acid appeared to correlate closely with copper tolerance. We conclude that the brown-rot fungi tested that were able to exceed and maintain an oxalic acid concentration of ⩾600 μmol / g effectively decayed SYP treated with CC.

Identifications of 30 species of polypores described by Corner were made by type examinations. The following new combinations are proposed: Antrodiella brunneimontana, A. diffluens, A. flavitubus, Ceriporiopsis aurantitingens, C. cremeicarnea, Dichomitus affixus, Oligoporus bambusicola, O. cretaceitextus, and Skeletocutis falsipileata. The following names are accepted in the original genus: Tyromyces corniculatus, T. corticola, T. hispidulinanus, T. inodermatus, T. interponens, and T. languidus. Tyromyces aurantilaetus was already combined with the proper genus. The following species are treated as synonyms: Tyromyces amyloideus, a synonym of Oligoporus caesius; T. citrinicarneus, a synonym of Rigidoporus dextrinoideus; T. coeruleivirens, a synonym of O. caesius; T. fagraeae, a synonym of T. corticola; T. flavidicera, a synonym of R. dextrinoideus; and T. inconsideratus, a synonym of Tinctoporellus epimiltinus. Tyromyces dianthicolor and T. favulus belong to difficult complexes, and allied species are cited for each species. The following species are dubious because of their poor or sterile condition: Tyromyces albovinaceus, T. carbonicola, T. dacrydii, and T. descendens. No authentic specimens were traced for T. cinnamomeiporus and T. citriniporus. Descriptions and line drawings are given for the accepted species.

Summary It is not definitively known whether or not the production of extracellular hydrogen peroxide (H2O2) is a universal characteristic of brown-rot fungi. Cytochemical localization of H2O2 was tested in two brown-rot fungi, Tyromyces palustris and Coniophora puteana, by staining with cerium chloride. Transmission electron microscopy (TEM) showed the deposition of cerium perhydroxide within the fungal hyphae as well as wood cell walls affected by brown-rot fungi. TEM work indicated that extracellular H2O2 was present in brown-rot fungi and that H2O2 from brown-rot fungi diffused into the wood cell walls in the early stages of decay. The present work strongly suggests that H2O2 plays an important role in the early degradation of cellulose by brown-rot fungi. The usefulness of this technique for localizing H2O2 at high resolution with minimal nonspecific deposition is also discussed.

New data on corticioid and poroid basidiomycetes from Kedrovaya Pad Nature Reserve, Primorye Territory, Russian Far East, is presented. Altogether there are 182 known species from the reserve; 76 are reported as new to the reserve and two as new to Russia. The material was collected in July of 2016 from 9 study plots located in forests of different types. Findings of some rarely collected species such as Botryobasidium botryoideum, Cerinomyces aff. aculeatus, Junghuhnia aurantilaeta, Phanerochaete robusta and Tyromyces wynneae are briefly discussed. The full-length ITS sequences of Junghuhnia aurantilaeta, Phanerochaete robusta, Pyrrhoderma cf. sendaiense and Tyromyces wynneae were obtained and deposited in the GenBank. Junghuhnia aurantilaeta is proposed to be included in the new edition of the Red Data Book of Primorye Territory. The paper expands the knowledge of distribution of many species and increases the total number of species reported for the reserve.

Phylogenies of Ceriporiopsis and related genera were studied using DNA sequences from the ITS+28S nuclear ribosomal RNA gene regions. Two species, Ceriporiopsis alboaurantia and C. semisupina, are described as new to science. Ceriporiopsis alboaurantia is characterized by annual growth habit, resupinate basidiocarps with white pore surface when fresh, turning to apricot-orange to dark orange upon drying, monomitic hyphal system with clamped generative hyphae encrusted with pale yellow crystals, ellipsoid and thin-walled basidiospores (4–5 ´ 3–3.3 µm), and plenty of pale-yellow to pale-orange oily substances in subiculum and trama. Ceriporiopsis semisupina is distinguished by annual, effused-reflexed basidiocarps with greyish brown to reddish brown pore surface, monomitic hyphal system with clamped generative hyphae bearing crystalline incrustation, long-clavate basidia, ellipsoid and thin-walled basidiospores (4–4.5 ´ 3–3.3 µm). Like previous study, the phylogenetic analysis presented here also showed that Ceriporiopsis is not monophyletic and the sampled species were grouped in four clades, including phlebia clade, residual polyporoid clade, tyromyces clade and gelatoporia clade.

Twenty-seven species of polypores described by Corner were identified by type examinations. Corneroporus gen. nov. is proposed, typified by Boletopsis subcitrina. The following new combinations are proposed: Coriolopsis lacunosa, Corneroporus subcitrinus, Heterobasidion arbitrarium, Rigidoporus dimiticus, Skeletocutis basifusca, Tyromyces dolosus, T. friabilis, T. hispidus, T. subochraceus. The following names are accepted in the original genera: Trichaptum agglutinatum, T. suberosum, and Gloeoporus sulphureus. Gloeoporus carri was already combined with the proper genus. The following species are synonyms: Trichaptum ceraceicutis, T. favoloides, Diacanthodes griseus, Elmerina unguliformis, Gloeoporus nigrescens, G. papuanus, G. pendens, G. umbrinus, and G. vitellinus. Gloeoporus cremeoalbus belongs to a difficult complex and an allied species is cited. The following species are dubious because of their poor or sterile conditions: Trichaptum jackiae and Fomitopsis euosma. No authentic specimens were traced for Albatrellus borneensis and Gloeoporus similis. Descriptions and line drawings are given for the accepted species.

The increased interest in copper-based wood preservatives has hastened the need for understanding why some fungi are able to attack copper-treated wood. Due in part to accumulation of oxalic acid by brown-rot fungi and visualization of copper oxalate crystals in wood decayed by known copper-tolerant decay fungi, oxalic acid has been implicated in copper tolerance by the formation of copper oxalate crystals. Nineteen isolates of the brown-rot fungus Wolfiporia cocos were evaluated for oxalic acid production and weight loss on wood treated with 1.2% copper citrate. Twelve of 19 isolates that caused moderate to high weight losses in copper citrate-treated wood produced low oxalic acid in liquid culture, whereas isolates with high oxalic acid production had low weight losses in treated wood. Seven W. cocos isolates demonstrated enhanced weight loss in Cu-treated wood. Wood weight loss was unaffected by the presence of copper citrate for two W. cocos isolates and weight loss was lower for 10 isolates compared to weight losses in untreated wood. Citrate did not significantly influence oxalic acid production in liquid culture. Previous hypotheses linking oxalic acid and copper tolerance were based upon observations of single isolates of Postia and Tyromyces. Although most isolates produced more oxalic acid in copper citrate-treated wood than in untreated wood, we found no statistical relationship between the amount of oxalic acid production in liquid culture or wood and copper tolerance in W. cocos. Production of oxalic acid does not seem to be the factor controlling copper tolerance in W. cocos. The diversity seen within W. cocos demonstrates that caution should be used when reporting results, so that generalizations are not based on the behavior of a single isolate.

The reduction of ferric chelate caused by various wood-rot fungi was analyzed. Ferric chelate reductive activity was detected in cell-free extracts of seven wood-rot fungi:Phanerochaete chrysosporium, P. sordida YK-624,Ganoderma sp. YK-505,Coriolus versicolor, Bjerkandera adusta, Tyromyces palustris, andGloeophyllum trabeum. These fungi produced NADPH- or NADH-dependent ferric chelate reductive enzymes (or both) of different molecular weight. In the liquid culture ofP. sordida YK-624 andC. versicolor, a positive correlation was observed between extracellular MnP activity and intracellular NADPH-dependent ferric chelate reductive activity.

Sequences of nuclear ITS (nrDNA) regions were obtained from 22 species of Tyromyces s.l., a group of important wood-rotting, poroid holobasidiomycetes, belonging to Coriolaceae. Different reference collections of a species generally produced identical molecular sequences. Parsimony analysis of these sequences resulted in trees compatible with the currently accepted delimitation of genera, but the alignment of sequences has proven to be difficult owing to regions of uncertainty. Analyses based on inclusion and exclusion of ambiguous regions in the alignment demonstrate that closely related taxes are, however, always clustered together, especially at the generic level. It is considered that ITS sequences are reliable in distinguishing genera and species of Tyromyces s.l., but not able to provide sufficient resolution for relationships between some formerly recognized genera. The taxonomic implications of ITS variation are also discussed. Species of Oligoporus and Postia clustered in one clade, indicating a close relationship as shown from morphological research. Species of Skeletocutis are characterized by encrusted hyphal ends in the hymenophore, but the type species of Tyromyces, T. chioneus, is embedded within the Skeletocutis clade, indicating a strong link between these two genera. Species of Antrodia and of Spongipellis included in this study were not well supported as monophyletic groups, although they are clustered together within the trees.

A new glyoxylate dehydrogenase which catalyzes dehydrogenation of glyoxylate to oxalate in the presence of cytochrome c has been purified as an electrophoretically homogeneous protein from the cell-free extracts of a wood-destroying basidiomycete Tyromyces palustris. The enzymatic reduction of cytochrome c was dependent on glyoxylate which was found to be the best substrate among the compounds tested. The Km value for glyoxylate was determined to be 2.7 mM at the optimal pH (8.0). The UV-visible spectra of the enzyme in oxidized and reduced forms indicate that the enzyme belongs to a family of flavohemoproteins. The flavin nucleotide isolated from the native enzyme by heat denaturation was identified as FMN. The enzyme (Mr 331000) consists of six identical homopolymers (Mr of subunit 59000), which were found to constitute a symmetric octahedral shape by electron-microscopic observation with a negative staining method.

Pure cultures of the Paria indoor brown-not fungi, Antrodia vaillantii, A.sinuosa, A.serialis, A.xantha and Tyromyces placenta, were studied regarding growth rate, response to temperature, copper tolerance, wood decay and mycelial interactions. Radial growth extension reached from 4-9 mm/d. All fungi were rather resistant to high temperatures. Optimum temperature was between 25 and 31°C. All withstood 1 hour at 60°C and some even 3h at 65°C. A.vaillantii showed copper tolerance up to 0.05 M Cu. Wood weight loss after 20 weeks was higher by T.placenta (35%) and A.sinuosa (33%) than by A.xantha (21%), A.serialis (16%) and A.vaillantii (14%). Dual cultures showed various inter- and intraspecific interactions and detected identity of differently coded cultures. The former Poria vaporaria Normstamm II for testing wood preservatives and the recent Poria placenta EN113 strain, FPRL280, were assumed either to be identical or at least to originate from the same individual.

A new species of Polyporales, named as Skeletocutis yunnanensis, was collected on angiosperm wood in northern Yunnan Province, southwestern China. It is described based on morphological characteristics and molecular evidence. The species belongs to the Skeletocutis subincarnata complex, but differs morphologically from all known species of the genus by white, cream to buff pores surface, angular pores mostly 5–6 per mm with entire mouths, a dimitic hyphal structure both in trama and subiculum, generative hyphae in whole basidiocarps covered by fine crystals, skeletal hyphae unchanged in KOH, not agglutinated, allantoid basidiospores measured as 3.5–4.5 × 1.0–1.2 µm, and growth on angiosperm wood. Phylogenetic analysis based on the internal transcribed spacer (ITS) regions and nuclear large subunit (nLSU) ribosomal RNA gene regions indicated that the new species grouped with Skeletocutis and nested in the tyromyces clade.

The taxonomic positions of several New Zealand polypore fungi were examined using morphological, cultural, sexual, and nuclear criteria. Postia venata (Rajchenb. & Wright) Rajchenb., previously described from Nothofagus forests in Argentina, is newly recorded in New Zealand on Nothofagus spp. It is distinct from Postia atrostrigosa (Cooke) comb. nov., which has a pileated fruit body and wider, strictly allantoid, IKI+ basidio-spores. Postia venata is also distinct from Tyromyces setiger (Cooke) G.H. Cunn., which has a pileated, uniformly strigose, white, triquetrous fruit body, and strictly allantoid spores. Skeletocutis stramenticus (G.H. Cunn.) comb. nov. is shown to be conspecific with S. australis Rajchenb., also reported from Argentina. Polyporus hemitephrus Berk, is a white rotting wood decay species and belongs in Fomes (Fr.) Fr. Cultural descriptions are given for Fomes hemitephrus (Berk.) Cooke, P. venata, and Schizopora nothofagi (G.H. Cunn.) P.K. Buchanan & Ryv. Cultural evidence suppo...

Species of the Poria group causing brown rot of wood in buildings and with similar morphological appearance and biology, namely Antrodia vaillantii, A. seriadis, A. sinuosa, A. xantha, and Tyromyces placenta, were investigated by SDS polyacrylamide gel electrophoresis. Different cultures of A. vaillantii showed a species-specific protein profile separating the fungus from A. serialis, A. sinuosa and A. xantha and from T. placenta. The method also differentiated these pore fungi from the dry rot fungus Serpula lacrymans and detected misidentifications

The boles of lodgepole pine trees (Pinus contorta murrayana) infected with heartwood decay fungi, emitted ethanol, a potential primary attractant for the mountain pine beetle (Dendroctonus ponderosae), at a higher rate (45.9 μg m−2 day−1) than uninfected trees (19.2 μg m−2 day−1) , on average. The average rate of emission of ethanol by the infected trees was not discernibly different from the average rate of emission of total monoterpenes (32.3 μg m−2 day−1) by these trees. However, the rate of emission of ethanol by uninfected trees exceeded that of total monoterpenes (6.8, μg m−2 day−1), on average. Decay fungi: Perenniporia subacida, Tyromyces sericeomollis and Phellinus pini, were identified in one or more of the decayed trees.

Copper remains the primary biocide component used today to protect wood. Increased interest in the use of non-arsenic copper-based wood preservatives has also led to increased studies on copper-tolerant decay fungi. Oxalic acid production by brown-rot fungi is proposed as one mechanism of copper tolerance. This study evaluated oxalic acid production and copper losses from ACQ- and CCA-treated Pinus sylvestris L. and Populus × euramericana I 214 wood by brown-rot fungi: Postia placenta, Gloeophyllum trabeum, Tyromyces palustris, and 2 strains of Serpula lacrymans. There was no clear linear correlation among mass losses, oxalic acid production, or copper losses in most cases. However, T. palustris and one strain of S. lacrymans showed copper tolerance in treated wood. P. placenta caused considerable mass losses in ACQ-treated specimens only. G. trabeum produced very low oxalic acid and was inhibited by ACQ and CCA wood preservatives. We concluded that wood species and preservative formulation affected the oxalic acid production, mass losses, and copper tolerance of the tested fungi.