1. Vitamin D and/or calcium deficient diets may differentially affect muscle fiber neuromuscular junction innervation
- Author
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Guilherme Rocha, Yanfei L. Ma, June Wiley, David J. Gifondorwa, Tyran D. Thompson, Henry Uhlman Bryant, Alexander E. Culver, Venkatesh Krishnan, and Pamela K. Shetler
- Subjects
0301 basic medicine ,Regulation of gene expression ,medicine.medical_specialty ,Physiology ,chemistry.chemical_element ,Parathyroid hormone ,Hindlimb ,Biology ,Calcium ,Neuromuscular junction ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,030104 developmental biology ,medicine.anatomical_structure ,Endocrinology ,chemistry ,Physiology (medical) ,Internal medicine ,Heat shock protein ,medicine ,Vitamin D and neurology ,Neurology (clinical) ,Ascorbic Acid Deficiency - Abstract
Introduction: There is evidence that supports a role for Vitamin D (Vit. D) in muscle. The exact mechanism by which Vit. D deficiency impairs muscle strength and function is not clear. Methods: Three-week-old mice were fed diets with varied combinations of Vit. D and Ca2+ deficiency. Behavioral testing, genomic and protein analysis, and muscle histology were performed with a focus on neuromuscular junction (NMJ)-related genes. Results: Vit. D and Ca2+ deficient mice performed more poorly on given behavioral tasks than animals with Vit. D deficiency alone. Genomic and protein analysis of the soleus and tibialis anterior muscles revealed changes in several Vit. D metabolic, NMJ-related, and protein chaperoning and refolding genes. Conclusions: These data suggest that detrimental effects of a Vit. D deficient or a Vit. D and Ca2+ deficient diet may be a result of differential alterations in the structure and function of the NMJ and a lack of a sustained stress response in muscles. This article is protected by copyright. All rights reserved.
- Published
- 2016
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