97 results on '"Tvedten, H."'
Search Results
2. Megakaryocyte cytoplasmic fragments and ragocytes in the peripheral blood of a domestic cat infected with Anaplasma.
- Author
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Tvedten, H.
- Subjects
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CATS , *ANAPLASMA , *RADIOACTIVE substances , *BLOOD sampling , *BLOOD platelets - Abstract
In this case report, the author reports three strong and highly unusual changes in the peripheral blood of a domestic cat infected with Anaplasma. A freshly made blood smear from the cat Anaplasma had a shower of about 100 variably large to huge cytoplasmic fragments of megakaryocyte cytoplasm along the feathered edge. These had budding of platelets at their margins. Neutrophils with red cytoplasmic inclusions resembling ragocytes and neutrophils with ingested round inclusions of nuclear material were seen in a 1-day-old EDTA blood sample but not the freshly made blood smear. The cells with nuclear material resembled LE cells or alternatively Tart cells. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
3. Detection of frequent neutrophil misclassification by the ProCyte Dx in sick dogs and how to avoid it
- Author
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Bergstrand, E., primary, Tvedten, H. W., additional, and Lilliehöök, I., additional
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- 2022
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4. Resection of a ceruminous adenocarcinoma in a horse by a modified vertical ear canal ablation
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Skärlina, E. M., Tvedten, H. W., Roberts, V. L. H., and Gorvy, D. A.
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- 2015
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- View/download PDF
5. AUTOMATIC DIGITAL IMAGE ANALYSIS SYSTEM (CELLAVISION DM96) USED FOR CANINE LEUKOCYTE DIFFERENTIAL COUNTING.: 6
- Author
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Lilliehook, I. and Tvedten, H.
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- 2009
6. Establishment of the European College of Veterinary Clinical Pathology (ECVCP) and the current status of veterinary clinical pathology in Europe
- Author
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O'Brien, P. J., Fournel-Fleury, C., Bolliger, A. P., Freeman, K. P., Braun, J.-P., Archer, J., Paltrinieri, S., Tvedten, H., Polizopoulou, Z. S., Jensen, A. L., Pastor, J., Lanevschi-Pietersma, A., Thoren-Tolling, K., Schwendenwien, I., Thoresen, S. I., Bauer, N. B., Ledieu, D., Cerón, J. J., Palm, M., Papasouliotis, K., Gaál, T., and Vajdovich, P.
- Published
- 2007
- Full Text
- View/download PDF
7. EVALUATION OF THE SYSMEX XT-2000iV HEMATOLOGY SYSTEM FOR USE WITH CANINE, FELINE AND EQUINE HEMATOLOGY TESTING.: 1
- Author
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Lilliehook, I. and Tvedten, H.
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- 2006
- Full Text
- View/download PDF
8. Feline Differential Leukocyte Count with ProCyte Dx : Frequency and Severity of a Neutrophil-Lymphocyte Error and How to Avoid It
- Author
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Tvedten, H. W., Andersson, V., Lilliehook, I. E., Tvedten, H. W., Andersson, V., and Lilliehook, I. E.
- Abstract
Background: Erroneous neutrophil and lymphocyte counts from analysis of feline blood samples were transferred directly into the hospital information system from the ProCyte Dx hematology instrument in our after-hours laboratory. Errors usually were not detected by the users. Hypothesis/Objectives: To quantify the frequency and severity of errors associated with the ProCyte Dx analyzer and to identify methods to avoid the errors. Animals: One-hundred six EDTA blood samples routinely submitted from feline hospital patients were analyzed. Methods: ProCyte differential leukocyte counts were compared to 2 reference methods: Advia 2120 hematology instrument and manual enumeration. Limits for unacceptable deviation from the reference methods were defined as 18 for % lymphocytes and 23 for % neutrophils. Results: Fourteen of 106 samples had unacceptable errors for both lymphocytes and neutrophils compared to both reference methods. Median % lymphocytes in those 14 samples were 11.2, 15.0, and 53.0% for Advia, manual, and ProCyte, respectively. Median % neutrophils were 85.4, 81.5, and 34.2% for Advia, manual, and ProCyte, respectively. All errors were avoided by rejecting automated ProCyte differential leukocyte results whenever the dot plot appeared clearly incorrect, but only 9 of these 14 samples had a ProCyte WBC distribution error flag. Conclusions and Clinical Importance: Results reported by ProCyte had markedly falsely increased lymphocyte and decreased neutrophil counts in 13% of feline patient samples. Users must reject automated differential leukocyte count results when the WBC dot plot appears overtly incorrect. Rejection based only on ProCyte WBC error flag was insufficient.
- Published
- 2017
- Full Text
- View/download PDF
9. Comparison of specific gravity analysis of feline and canine urine, using five refractometers, to pycnometric analysis and total solids by drying
- Author
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Tvedten, H. W., Ouchterlony, Hanna, Lilliehook, I. E., Tvedten, H. W., Ouchterlony, Hanna, and Lilliehook, I. E.
- Abstract
AIMS: To compare the performance of five refractometers for determination of urine specific gravity in cats and dogs, with reference to weight of total solids and pycnometer analysis. METHODS: Urine samples from 27 cats and 31 dogs submitted for routine urinalysis were included. Urine specific gravity was determined with five refractometers. Four were optical, hand-held refractometers with a temperature compensation method and one was a digital model. Urine was dried to determine the precise weight of total solids. The total solids (g/L) were converted to an estimated specific gravity by division with 2.33. Urine specific gravity of four feline and seven canine samples were analysed with a pycnometer. Limits of agreement analysis was used to evaluate the agreement between specific gravity (analysed as specific gravity minus 1) measured by the refractometers and estimated from dried total solids, or pycnometer results. RESULTS: The five refractometers reported clearly different results from each other. Proportional negative bias was noted for refractometer results compared to estimated specific gravity from total solids and a constant negative bias compared to pycnometer results. The two refractometers designed for cat urine reported similar and lowest specific gravity results with a mean negative bias of 0.007 and 0.008 units compared to estimated specific gravity from total solids, and a mean negative bias of 0.006 units compared to pycnometer results. CONCLUSIONS: Refractometer results did not increase consistently with increasing urine specific gravity compared to reference methods or to other refractometers. Two feline refractometers reported consistently lower specific gravity results than reference methods and other refractometers. CLINICAL RELEVANCE: Because of this imprecision, veterinarians should not use precise cut off values such as 1.030 or 1.035 for evaluation of renal concentrating ability in dogs and cats. Veterinarians should consider the variabilit
- Published
- 2015
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10. Serum Serotonin Concentration Is Associated with Severity of Myxomatous Mitral Valve Disease in Dogs
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Ljungvall, I., primary, Höglund, K., additional, Lilliehöök, I., additional, Oyama, M.A., additional, Tidholm, A., additional, Tvedten, H., additional, and Häggström, J., additional
- Published
- 2013
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11. Establishment of the European College of Veterinary Clinical Pathology (ECVCP) and the current status of veterinary clinical pathology in Europe
- Author
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O'Brien, P.J., Fournel-Fleury, C., Bolliger, Adrian Marc, Freeman, K.P., Braun, J.-P., Archer, J., Paltrinieri, S., Tvedten, H., Polizopoulou, Z.S., Jensen, Asger Lundorff, Pastor, J., Lanevschi-Pietersma, A., Thoren-Tolling, K., Schwendenwien, I., Thoresen, S.I., Bauer, N.B., Ledieu, D., Cerón, J.J., Palm, M., Papasouliotis, K., Gaál, T., Vajdovich, P., O'Brien, P.J., Fournel-Fleury, C., Bolliger, Adrian Marc, Freeman, K.P., Braun, J.-P., Archer, J., Paltrinieri, S., Tvedten, H., Polizopoulou, Z.S., Jensen, Asger Lundorff, Pastor, J., Lanevschi-Pietersma, A., Thoren-Tolling, K., Schwendenwien, I., Thoresen, S.I., Bauer, N.B., Ledieu, D., Cerón, J.J., Palm, M., Papasouliotis, K., Gaál, T., and Vajdovich, P.
- Abstract
After 5 years of development, the European College of Veterinary Clinical Pathology (ECVCP)was formally recognized and approved on July 4, 2007 by the European Board of Veterinary Specialisation (EBVS), the European regulatory body that oversees specialization in veterinary medicine and which has approved 23 colleges. The objectives, committees, basis for membership, constitution, bylaws, information brochure and certifying examination of the ECVCP have remained unchanged during this time except as directed by EBVS. The ECVCP declared full functionality based on the following criteria: 1) a critical mass of 65 members: 15 original diplomates approved by theEBVS to establish theECVCP, 37 de facto diplomates, 7 diplomates certified by examination, and 5 elected honorary members; 2) the development and certification of training programs, laboratories, and qualified supervisors for residents; currently there are 18 resident trainingprograms inEurope; 3) administration of 3 annual board-certifying examinations thus far,with an overall pass rate of 70%; 4) European consensus criteria for assessing the continuing education of specialists every 5 ears; 5) organization of 8 annual scientific congresses and a joint journal (with the American Society for Veterinary Clinical Pathology) for communication of scientific research and information; the College also maintains a website, a joint listserv, and a newsletter; 6) collaboration in training and continuing education with relevant colleges in medicine and pathology; 7) development and strict adherence to a constitution and bylaws compliantwith the EBVS; and 8) emonstration of compelling rationale, supporting data, and the support ofmembersandother colleges for independence as a specialtycollege.FormalEBVS recognitionofECVCPas the regulatory body for the science and practice of veterinary clinical pathology in Europewill facilitate growth and development of the discipline and compliance of academic, commercial diagnostic, and i
- Published
- 2007
12. Classification of Canine Malignant Lymphomas According to the World Health Organization Criteria
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Valli, V. E., primary, Myint, M. San, additional, Barthel, A., additional, Bienzle, D., additional, Caswell, J., additional, Colbatzky, F., additional, Durham, A., additional, Ehrhart, E. J., additional, Johnson, Y., additional, Jones, C., additional, Kiupel, M., additional, Labelle, P., additional, Lester, S., additional, Miller, M., additional, Moore, P., additional, Moroff, S., additional, Roccabianca, P., additional, Ramos-Vara, J., additional, Ross, A., additional, Scase, T., additional, Tvedten, H., additional, and Vernau, W., additional
- Published
- 2010
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13. Diseases associated with pronounced eosinophilia: a study of 105 dogs in Sweden
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Lilliehöök, I., primary, Gunnarsson, L., additional, Zakrisson, G., additional, and Tvedten, H., additional
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- 2000
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14. The Sensitivity and Specificity of Radiology and Histopathology in the Diagnosis of Posttraumatic Osteomyelitis
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Braden, T. D., Tvedten, H. W., Mostosky, U. V., Thomas, M., Stickle, R. L., and Kaneene, J. B.
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- 1989
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15. The Sensitivity and Specificity of Radiology and Histopathology in the Diagnosis of Posttraumatic Osteomyelitis
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Tvedten, H. W., primary, Mostosky, U. V., primary, Thomas, M., primary, Stickle, R. L., primary, Kaneene, J. B., primary, and Braden, T. D., additional
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- 1989
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16. Comparison of specific gravity analysis of feline and canine urine, using five refractometers, to pycnometric analysis and total solids by drying
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Tvedten, H.W, Ouchterlony, H, and Lilliehöök, I.E
- Published
- 2015
17. International Guidelines for Veterinary Tumor Pathology: A Call to Action
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Michelle M. Dennis, Robert Klopfleisch, Rebecca C. Smedley, Matti Kiupel, Giancarlo Avallone, Elisa N. Salas, Laura Marconato, Renée Laufer-Amorim, Paul C. Stromberg, Richard Luong, Michael J. Dark, Christof A. Bertram, Robert A. Foster, Milan Milovancev, Frances M. Moore, Bruce Williams, Derick B. Whitley, John M. Cullen, Nick Dervisis, Harold Tvedten, Keith E. Linder, Marc Aubreville, D. Glen Esplin, Margaret A. Miller, Michael H. Goldschmidt, Renato L. Santos, Marlene L. Hauck, Linden E. Craig, Midori G. Asakawa, Andrew D. Miller, Jeanne W. George, D. J. Meuten, Michelle L. Oblak, Kristina Meichner, John S. Munday, Yumiko Kagawa, Pompei Bolfa, R. Mark Simpson, Paola Roccabianca, F. Yvonne Schulman, Taryn A. Donovan, Meuten D.J., Moore F.M., Donovan T.A., Bertram C.A., Klopfleisch R., Foster R.A., Smedley R.C., Dark M.J., Milovancev M., Stromberg P., Williams B.H., Aubreville M., Avallone G., Bolfa P., Cullen J., Dennis M.M., Goldschmidt M., Luong R., Miller A.D., Miller M.A., Munday J.S., Roccabianca P., Salas E.N., Schulman F.Y., Laufer-Amorim R., Asakawa M.G., Craig L., Dervisis N., Esplin D.G., George J.W., Hauck M., Kagawa Y., Kiupel M., Linder K., Meichner K., Marconato L., Oblak M.L., Santos R.L., Simpson R.M., Tvedten H., and Whitley D.
- Subjects
medicine.medical_specialty ,Standardization ,040301 veterinary sciences ,Reproducibility of Result ,0403 veterinary science ,03 medical and health sciences ,0302 clinical medicine ,Neoplasms ,medicine ,Animals ,Medical physics ,protocol ,Grading (tumors) ,Pathology, Veterinary ,standardization ,validation ,General Veterinary ,business.industry ,Animal ,Reproducibility of Results ,Foundation (evidence) ,Veterinary tumor ,04 agricultural and veterinary sciences ,Call to action ,030220 oncology & carcinogenesis ,oncology ,Neoplasm ,business ,guideline - Abstract
Standardization of tumor assessment lays the foundation for validation of grading systems, permits reproducibility of oncologic studies among investigators, and increases confidence in the significance of study results. Currently, there is minimal methodological standardization for assessing tumors in veterinary medicine, with few attempts to validate published protocols and grading schemes. The current article attempts to address these shortcomings by providing standard guidelines for tumor assessment parameters and protocols for evaluating specific tumor types. More detailed information is available in the Supplemental Files, the intention of which is 2-fold: publication as part of this commentary, but more importantly, these will be available as “living documents” on a website ( www.vetcancerprotocols.org ), which will be updated as new information is presented in the peer-reviewed literature. Our hope is that veterinary pathologists will agree that this initiative is needed, and will contribute to and utilize this information for routine diagnostic work and oncologic studies. Journal editors and reviewers can utilize checklists to ensure publications include sufficient detail and standardized methods of tumor assessment. To maintain the relevance of the guidelines and protocols, it is critical that the information is periodically updated and revised as new studies are published and validated with the intent of providing a repository of this information. Our hope is that this initiative (a continuation of efforts published in this journal in 2011) will facilitate collaboration and reproducibility between pathologists and institutions, increase case numbers, and strengthen clinical research findings, thus ensuring continued progress in veterinary oncologic pathology and improving patient care.
- Published
- 2021
18. International Guidelines for Veterinary Tumor Pathology: A Call to Action.
- Author
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Meuten DJ, Moore FM, Donovan TA, Bertram CA, Klopfleisch R, Foster RA, Smedley RC, Dark MJ, Milovancev M, Stromberg P, Williams BH, Aubreville M, Avallone G, Bolfa P, Cullen J, Dennis MM, Goldschmidt M, Luong R, Miller AD, Miller MA, Munday JS, Roccabianca P, Salas EN, Schulman FY, Laufer-Amorim R, Asakawa MG, Craig L, Dervisis N, Esplin DG, George JW, Hauck M, Kagawa Y, Kiupel M, Linder K, Meichner K, Marconato L, Oblak ML, Santos RL, Simpson RM, Tvedten H, and Whitley D
- Subjects
- Animals, Reproducibility of Results, Neoplasms diagnosis, Neoplasms veterinary, Pathology, Veterinary
- Abstract
Standardization of tumor assessment lays the foundation for validation of grading systems, permits reproducibility of oncologic studies among investigators, and increases confidence in the significance of study results. Currently, there is minimal methodological standardization for assessing tumors in veterinary medicine, with few attempts to validate published protocols and grading schemes. The current article attempts to address these shortcomings by providing standard guidelines for tumor assessment parameters and protocols for evaluating specific tumor types. More detailed information is available in the Supplemental Files, the intention of which is 2-fold: publication as part of this commentary, but more importantly, these will be available as "living documents" on a website (www.vetcancerprotocols.org), which will be updated as new information is presented in the peer-reviewed literature. Our hope is that veterinary pathologists will agree that this initiative is needed, and will contribute to and utilize this information for routine diagnostic work and oncologic studies. Journal editors and reviewers can utilize checklists to ensure publications include sufficient detail and standardized methods of tumor assessment. To maintain the relevance of the guidelines and protocols, it is critical that the information is periodically updated and revised as new studies are published and validated with the intent of providing a repository of this information. Our hope is that this initiative (a continuation of efforts published in this journal in 2011) will facilitate collaboration and reproducibility between pathologists and institutions, increase case numbers, and strengthen clinical research findings, thus ensuring continued progress in veterinary oncologic pathology and improving patient care.
- Published
- 2021
- Full Text
- View/download PDF
19. Mitotic Figures-Normal, Atypical, and Imposters: A Guide to Identification.
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Donovan TA, Moore FM, Bertram CA, Luong R, Bolfa P, Klopfleisch R, Tvedten H, Salas EN, Whitley DB, Aubreville M, and Meuten DJ
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- Animals, Eosine Yellowish-(YS), Hematoxylin, Mitotic Index veterinary, Reproducibility of Results, Software
- Abstract
Counting mitotic figures (MF) in hematoxylin and eosin-stained histologic sections is an integral part of the diagnostic pathologist's tumor evaluation. The mitotic count (MC) is used alone or as part of a grading scheme for assessment of prognosis and clinical decisions. Determining MCs is subjective, somewhat laborious, and has interobserver variation. Proposals for standardizing this parameter in the veterinary field are limited to terminology (use of the term MC) and area (MC is counted in an area measuring 2.37 mm
2 ). Digital imaging techniques are now commonplace and widely used among veterinary pathologists, and field of view area can be easily calculated with digital imaging software. In addition to standardizing the methods of counting MF, the morphologic characteristics of MF and distinguishing atypical mitotic figures (AMF) versus mitotic-like figures (MLF) need to be defined. This article provides morphologic criteria for MF identification and for distinguishing normal phases of MF from AMF and MLF. Pertinent features of digital microscopy and application of computational pathology (CPATH) methods are discussed. Correct identification of MF will improve MC consistency, reproducibility, and accuracy obtained from manual (glass slide or whole-slide imaging) and CPATH approaches.- Published
- 2021
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20. Massive uric acid crystalluria and cylinduria in a dog after l-asparaginase treatment for lymphoma.
- Author
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Tvedten H, Lilliehöök I, Rönnberg H, and Pelander L
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- Animals, Asparaginase therapeutic use, Crystallization, Dog Diseases chemically induced, Dog Diseases drug therapy, Dogs, Female, Lymphoma complications, Lymphoma drug therapy, Lymphoma urine, Uric Acid blood, Urinalysis veterinary, Asparaginase adverse effects, Dog Diseases urine, Lymphoma veterinary, Uric Acid urine
- Abstract
A 10-year-old golden retriever bitch was treated for diarrhea and vomiting that lasted about 1 month without a specific diagnosis until a hepatic biopsy provided a histopathologic diagnosis of lymphoma. The dog was referred to the Swedish University of Agricultural Science and treated with one dose of l-asparaginase. The day after chemotherapy, the urine was dark yellow, very turbid, and had large amounts of small amorphous crystals and many casts made of similar appearing material identified by infrared spectroscopy to be 100% uric acid dihydrate. Serum uric acid was elevated at 224 μmol/L (RI 0-59). The dog's illness became worse after chemotherapy. Lymphoma treatment was not continued, and the dog was euthanized 9 days after the l-asparaginase treatment. Among other problems were persistent proteinuria with a urine protein-to-creatinine ratio of 2.3 and severe hypoalbuminemia. Serum protein electrophoresis performed 3 weeks prior to chemotherapy indicated hyperproteinemia (total protein 78 g/L) having a biclonal gammopathy with 35 g/L β-2 globulins and 11 g/L γ globulins. Despite prominent cylinduria and crystalluria, the patient did not develop azotemia or isosthenuria., (© 2019 American Society for Veterinary Clinical Pathology.)
- Published
- 2019
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21. Feline Differential Leukocyte Count with ProCyte Dx: Frequency and Severity of a Neutrophil-Lymphocyte Error and How to Avoid It.
- Author
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Tvedten HW, Andersson V, and Lilliehöök IE
- Subjects
- Animals, Hematology methods, Leukocyte Count instrumentation, Leukocyte Count methods, Lymphocytes, Neutrophils, Quality Control, Reproducibility of Results, Sensitivity and Specificity, Cats blood, Hematology instrumentation, Leukocyte Count veterinary
- Abstract
Background: Erroneous neutrophil and lymphocyte counts from analysis of feline blood samples were transferred directly into the hospital information system from the ProCyte Dx hematology instrument in our after-hours laboratory. Errors usually were not detected by the users., Hypothesis/objectives: To quantify the frequency and severity of errors associated with the ProCyte Dx analyzer and to identify methods to avoid the errors., Animals: One-hundred six EDTA blood samples routinely submitted from feline hospital patients were analyzed., Methods: ProCyte differential leukocyte counts were compared to 2 reference methods: Advia 2120 hematology instrument and manual enumeration. Limits for unacceptable deviation from the reference methods were defined as 18 for % lymphocytes and 23 for % neutrophils., Results: Fourteen of 106 samples had unacceptable errors for both lymphocytes and neutrophils compared to both reference methods. Median % lymphocytes in those 14 samples were 11.2, 15.0, and 53.0% for Advia, manual, and ProCyte, respectively. Median % neutrophils were 85.4, 81.5, and 34.2% for Advia, manual, and ProCyte, respectively. All errors were avoided by rejecting automated ProCyte differential leukocyte results whenever the dot plot appeared clearly incorrect, but only 9 of these 14 samples had a ProCyte WBC distribution error flag., Conclusions and Clinical Importance: Results reported by ProCyte had markedly falsely increased lymphocyte and decreased neutrophil counts in 13% of feline patient samples. Users must reject automated differential leukocyte count results when the WBC dot plot appears overtly incorrect. Rejection based only on ProCyte WBC error flag was insufficient., (Copyright © 2017 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals, Inc. on behalf of the American College of Veterinary Internal Medicine.)
- Published
- 2017
- Full Text
- View/download PDF
22. Measurement of serum C-reactive protein concentration for discriminating between suppurative arthritis and osteoarthritis in dogs.
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Hillström A, Bylin J, Hagman R, Björhall K, Tvedten H, Königsson K, Fall T, and Kjelgaard-Hansen M
- Subjects
- Animals, Arthritis, Infectious blood, Diagnosis, Differential, Dog Diseases blood, Dogs, Female, Male, Osteoarthritis blood, Prospective Studies, Synovial Fluid metabolism, Arthritis, Infectious veterinary, C-Reactive Protein metabolism, Dog Diseases diagnosis, Osteoarthritis veterinary
- Abstract
Background: In a dog with joint pain, it is important to determine whether it has suppurative joint disease, characterized by exudation of neutrophils in the synovial fluid, or not, as this affects choice of diagnostic tests and treatments. The aim of this study was to evaluate whether measurement of serum C-reactive protein (CRP) concentration could be used to discriminate between dogs with suppurative arthritis and osteoarthritis (OA). Furthermore, the concentrations of serum and synovial fluid interleukin (IL) 6 concentrations were measured in dogs with joint disease and in healthy dogs, and were correlated to serum CRP concentrations., Methods: Dogs with joint pain were enrolled prospectively and were classified to have suppurative arthritis or OA based on synovial fluid analysis and radiographic/arthroscopic findings. Healthy Beagles were enrolled as a comparative group. CRP and IL-6 concentrations were measured with canine-specific immunoassays. The performance of CRP concentration in discriminating between dogs with suppurative arthritis and OA was evaluated using a previously established clinical decision limit for CRP (20 mg/l), and by receiver operator characteristic (ROC) curve and logistic regression analysis. Comparisons of CRP and IL-6 concentrations between groups were performed using t-tests, and correlations by Spearman rank correlation coefficients., Results: Samples were obtained from 31 dogs with suppurative arthritis, 34 dogs with OA, and 17 healthy dogs. Sixty-two out of 65 dogs with joint disease were correctly classified using the clinical decision limit for CRP. Evaluation of ROC curve and regression analysis indicated that serum CRP concentrations could discriminate between suppurative arthritis and OA. Dogs with suppurative arthritis had higher serum CRP and serum and synovial fluid IL-6 concentrations compared to dogs with OA (p < 0.001). Dogs with OA had higher synovial fluid IL-6 concentrations (p < 0.001), but not higher serum CRP (p = 0.29) or serum IL-6 (p = 0.07) concentrations, compared to healthy dogs. There was a positive correlation between synovial fluid IL-6 and serum CRP concentrations (r
s = 0.733, p < 0.001), and between serum IL-6 and serum CRP concentrations (rs = 0.729, p < 0.001)., Conclusion: CRP concentration was found to discriminate well between dogs with suppurative arthritis and OA.- Published
- 2016
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23. Urine total protein concentration in clinically normal dogs.
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Tvedten H
- Published
- 2016
- Full Text
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24. Letter to the Editor.
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Hillström A and Tvedten H
- Published
- 2016
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25. Cytologic appearance of a meningioma recurring over 4 years after initial diagnosis.
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Tvedten H and Falkenö U
- Subjects
- Animals, Dog Diseases pathology, Exophthalmos veterinary, Meningeal Neoplasms veterinary, Meningioma veterinary, Orbital Neoplasms veterinary, Retina pathology
- Published
- 2015
- Full Text
- View/download PDF
26. Validation of a commercially available automated canine-specific immunoturbidimetric method for measuring canine C-reactive protein.
- Author
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Hillström A, Hagman R, Tvedten H, and Kjelgaard-Hansen M
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- Animals, Autoanalysis veterinary, Cross Reactions, Dogs, Female, Immunoassay methods, Immunoassay veterinary, Male, Nephelometry and Turbidimetry methods, Nephelometry and Turbidimetry veterinary, Protein Stability, Reference Values, Reproducibility of Results, Species Specificity, Acute-Phase Proteins analysis, C-Reactive Protein analysis, Dog Diseases blood, Dog Diseases diagnosis
- Abstract
Background: Measurement of C-reactive protein (CRP) is used for diagnosing and monitoring systemic inflammatory disease in canine patients. An automated human immunoturbidimetric assay has been validated for measuring canine CRP, but cross-reactivity with canine CRP is unpredictable., Objective: The purpose of the study was to validate a new automated canine-specific immunoturbidimetric CRP method (Gentian cCRP)., Methods: Studies of imprecision, accuracy, prozone effect, interference, limit of quantification, and stability under different storage conditions were performed. The new method was compared with a human CRP assay previously validated for canine CRP determination. Samples from 40 healthy dogs were analyzed to establish a reference interval., Results: Total imprecision was < 2.4% for 4 tested serum pools analyzed twice daily over 10 days. The method was linear under dilution, and no prozone effect was detected at a concentration of 1200 mg/L. Recovery after spiking serum with purified canine CRP at 2 different concentrations was 123% and 116%, respectively. No interference from hemoglobin or triglycerides (10 g/L) was detected. CRP was stable for 14 days at 4°C and 22°C. In the method comparison study, there was good agreement between the validated human CRP assay and the new canine-specific assay. Healthy dogs had CRP concentrations that were less than the limit of quantification of the Gentian cCRP method (6.8 mg/L)., Conclusions: The new canine-specific immunoturbidimetric CRP assay is a reliable and rapid method for measuring canine CRP, suitable for clinical use due to the option for an automated assay., (© 2014 The Authors Veterinary Clinical Pathology published by Wiley Periodicals, Inc. on behalf of American Society for Veterinary Clinical Pathology and European Society for Veterinary Clinical Pathology.)
- Published
- 2014
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27. Cytologic appearance of retinal cells included in a fine-needle aspirate of a meningioma around the optic nerve of a dog.
- Author
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Tvedten H and Hillström A
- Subjects
- Animals, Biopsy, Fine-Needle veterinary, Dog Diseases etiology, Dog Diseases surgery, Dogs, Exophthalmos etiology, Exophthalmos pathology, Exophthalmos surgery, Meningeal Neoplasms pathology, Meningeal Neoplasms surgery, Meningioma pathology, Meningioma secondary, Meningioma surgery, Optic Nerve pathology, Orbital Neoplasms complications, Orbital Neoplasms secondary, Retinal Rod Photoreceptor Cells pathology, Treatment Outcome, Dog Diseases pathology, Exophthalmos veterinary, Meningeal Neoplasms veterinary, Meningioma veterinary, Orbital Neoplasms veterinary, Retina pathology
- Abstract
A 6-year-old Wirehair Dachshund had a meningioma around the optic nerve that caused exophthalmos. A benign mesenchymal tumor was suspected based on the cytologic pattern of a fine-needle aspirate, and a meningioma was diagnosed by histopathologic examination. In addition to the meningioma cells, the cytologic smears included groups of cells from apparently 4 layers of normal retina. In particular, uniform rod-shaped structures in the cytologic sample could suggest rod-shaped bacteria, but these structures were identified as cylindrical outer segments of photoreceptor rod cells. Other retinal structures recognized included pigmented epithelial layer cells with their uniquely formed pigment granules, the characteristic bi-lobed, cleaved nuclei from the outer nuclear layer, and nerve tissue likely from the outer plexiform layer of the retina., (© 2013 American Society for Veterinary Clinical Pathology.)
- Published
- 2013
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28. Evaluation of cytologic findings in feline conjunctivitis.
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Hillström A, Tvedten H, Källberg M, Hanås S, Lindhe A, and Holst BS
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- Animals, Cat Diseases diagnosis, Cats, Chlamydophila classification, Chlamydophila Infections diagnosis, Chlamydophila Infections pathology, Conjunctivitis pathology, Female, Herpesviridae Infections diagnosis, Herpesviridae Infections pathology, Male, Mycoplasma classification, Mycoplasma Infections diagnosis, Mycoplasma Infections pathology, Real-Time Polymerase Chain Reaction, Cat Diseases pathology, Chlamydophila Infections veterinary, Conjunctivitis veterinary, Herpesviridae Infections veterinary, Mycoplasma Infections veterinary
- Abstract
Background: Cytologic examination of smears prepared from ocular swabs of conjunctiva from cats with conjunctivitis permits identification of the type of inflammation and possibly specific microorganisms. Results of studies of the diagnostic utility of cytology for detection of infectious causes of feline conjunctivitis have been inconsistent., Objectives: The objectives of this study were to describe cytologic findings in cats with conjunctivitis and to compare those findings with results of PCR analysis for feline herpesvirus (FHV-1), Chlamydophila felis (C felis), and Mycoplasma felis (M felis)., Methods: Conjunctival smears from 88 cats with conjunctivitis and 10 healthy control cats were stained with a Romanowsky stain and evaluated for the type of inflammation and evidence of an infectious agent. PCR analysis for FHV-1, C felis, and M felis was performed., Results: Infectious agents identified by PCR analysis were FHV-1 in 9 cats (10%), C felis in 8 cats (9%), and M felis in 6 cats (7%). Inclusions interpreted as chlamydial inclusions were found in all cytologic smears from cats positive for C felis by PCR analysis and in 3 PCR-negative cats. Inclusions interpreted as Mycoplasma organisms were found in 3 of 6 cats that were PCR-positive for M felis and in 1 PCR-negative cat. FHV-1 inclusion bodies were not detected on cytologic examination., Conclusions: Cytologic examination can be diagnostic for C felis infection when many typical inclusions are present. Cytologic examination was unreliable in diagnosing M felis infection, and viral inclusions of FHV-1 were not found in specimens stained with Romanowsky stains., (© 2012 American Society for Veterinary Clinical Pathology.)
- Published
- 2012
- Full Text
- View/download PDF
29. What is your diagnosis? Discrepancy between Sysmex XT-2000iV reticulocyte count and polychromasia.
- Author
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Tvedten H
- Subjects
- Animals, Dog Diseases blood, Dogs, Hematologic Tests instrumentation, Hematologic Tests veterinary, Leukemia blood, Leukemia diagnosis, Reticulocyte Count instrumentation, Reticulocytosis, Dog Diseases diagnosis, Leukemia veterinary, Reticulocyte Count veterinary, Reticulocytes pathology
- Published
- 2011
- Full Text
- View/download PDF
30. Use of prostaglandin E1 to obtain more accurate platelet counts in cats.
- Author
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Tvedten H and Johansson P
- Subjects
- Animals, Blood Specimen Collection methods, Blood Specimen Collection veterinary, Edetic Acid, Platelet Count methods, Alprostadil, Cats blood, Platelet Count veterinary
- Published
- 2011
- Full Text
- View/download PDF
31. Hereditary phosphofructokinase deficiency in wachtelhunds.
- Author
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Hillström A, Tvedten H, Rowe A, and Giger U
- Subjects
- Anemia, Hemolytic, Congenital blood, Anemia, Hemolytic, Congenital veterinary, Animals, Dog Diseases blood, Dogs, Female, Genetic Predisposition to Disease, Dog Diseases genetics, Erythrocytes enzymology, Phosphofructokinases deficiency
- Abstract
Hereditary phosphofructokinase (PFK) deficiency was diagnosed in two Wachtelhund dogs and suspected in three related Wachtelhund dogs with exercise intolerance, hemolytic anemia, and pigmenturia. Severe, persistent reticulocytosis in light of only mild anemia together with hemoglobinuria after strenuous exercise suggested PFK deficiency. Low erythrocyte PFK activity together with low 2,3-diphosphoglycerate concentrations and a high hemoglobin-oxygen affinity confirmed the diagnosis. The PFK deficiency is due to a single missense mutation in the muscle-type PFK M-PFK gene in English springer and American cocker spaniels, whippets, and mixed-breed dogs; however, these PFK-deficient Wachtelhunds do not have the same PFK mutation.
- Published
- 2011
- Full Text
- View/download PDF
32. Classification of canine malignant lymphomas according to the World Health Organization criteria.
- Author
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Valli VE, San Myint M, Barthel A, Bienzle D, Caswell J, Colbatzky F, Durham A, Ehrhart EJ, Johnson Y, Jones C, Kiupel M, Labelle P, Lester S, Miller M, Moore P, Moroff S, Roccabianca P, Ramos-Vara J, Ross A, Scase T, Tvedten H, and Vernau W
- Subjects
- Animals, Dogs, Lymph Nodes pathology, Lymphoma classification, Observer Variation, Pathology, Veterinary standards, Veterinarians standards, World Health Organization, Dog Diseases classification, Lymphoma veterinary
- Abstract
A study was carried out to test the accuracy and consistency of veterinary pathologists, not specialists in hematopathology, in applying the World Health Organization (WHO) system of classification of canine lymphomas. This study represents an initiative of the ACVP Oncology Committee, and the classification has been endorsed by the World Small Animal Veterinary Association (WASVA). Tissue biopsies from cases of canine lymphoma were received from veterinary oncologists, and a study by pathologists given only signalment was carried out on 300 cases. Twenty pathologists reviewed these 300 cases with each required to choose a diagnosis from a list of 43 B and T cell lymphomas. Three of the 20 were hematopathologists who determined the consensus diagnosis for each case. The 17 who formed the test group were experienced but not specialists in hematopathology, and most were diplomates of the American or European Colleges of Veterinary Pathology. The overall accuracy of the 17 pathologists on the 300 cases was 83%. When the analysis was limited to the 6 most common diagnoses, containing 80% of all cases, accuracy rose to 87%. In a test of reproducibility enabled by reintroducing 5% of cases entered under a different identity, the overall agreement between the first and second diagnosis ranged from 40 to 87%. The statistical review included 43,000 data points for each of the 20 pathologists.
- Published
- 2011
- Full Text
- View/download PDF
33. What is your diagnosis? Discrepancy in platelet counts determined using a Sysmex XT-2000 iV hematology analyzer. Erroneous PLT-O due to RBC ghosts.
- Author
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Tvedten H
- Subjects
- Anemia, Hemolytic, Autoimmune blood, Anemia, Hemolytic, Autoimmune diagnosis, Animals, Dog Diseases diagnosis, Dogs, False Positive Reactions, Female, Anemia, Hemolytic, Autoimmune veterinary, Autoanalysis veterinary, Dog Diseases blood, Erythrocyte Membrane, Platelet Count veterinary
- Published
- 2010
- Full Text
- View/download PDF
34. Feline platelet counting with prostaglandin E1 on the Sysmex XT-2000iV.
- Author
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Tvedten H and Johansson P
- Subjects
- Animals, Autoanalysis instrumentation, Autoanalysis methods, Autoanalysis veterinary, Cat Diseases blood, Cat Diseases diagnosis, Edetic Acid pharmacology, Platelet Aggregation drug effects, Platelet Count instrumentation, Platelet Count methods, Thrombocytopenia blood, Thrombocytopenia diagnosis, Thrombocytopenia veterinary, Alprostadil pharmacology, Cats blood, Platelet Count veterinary
- Abstract
Background: The large size of many feline platelets and the high frequency of platelet aggregation often results in falsely low platelet counts in this species. A combination of optical platelet counting to detect even large platelets and the use of prostaglandin E1 (PGE1) to inhibit platelet clumping may increase the accuracy of feline platelet counting., Objective: The objective of this study was to compare platelet counts in feline whole blood samples with and without the addition of PGE1 and using different analytical methods in a clinical setting., Methods: Platelet counts were determined in 10 feline patients in a referral veterinary hospital using 2 sample types (EDTA, EDTA with PGE1) and 2 methods of analysis (optical counting [PLT-O] and impedance counting [PLT-I]) on the Sysmex XT 2000 iV analyzer., Results: All PGE1-PLT-O samples had platelet counts of >200 x 10(9)/L. Mean platelet count using PGE1-PLT-O (410,256+/-178 x 10(9)/L) was significantly higher (P<.03) compared with PGE1-PLT-I (256+/-113 x 10(9)/L), EDTA-PLT-O (238+/-107 x 10(9)/L), and EDTA-PLT-I (142+/-84 x 10(9)/L) methods. Depending on the method, platelet counts in 2 to 7 of 10 cats were <200 x 10(9)/L when PGE1-PLT-O was not used. A slightly increased platelet count in response to treatment of a feline patient with thrombocytopenia would have been missed without use of PGE1-PLT-O., Conclusions: Using PLT-O analysis on EDTA samples containing PGE1 provides higher, and therefore likely more accurate, feline platelet counts in a clinical setting.
- Published
- 2010
- Full Text
- View/download PDF
35. Evaluation of an in-clinic Serum Amyloid A (SAA) assay and assessment of the effects of storage on SAA samples.
- Author
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Hillström A, Tvedten H, and Lilliehöök I
- Subjects
- Animals, Diagnostic Tests, Routine methods, Horses, Inflammation diagnosis, Reproducibility of Results, Sensitivity and Specificity, Temperature, Time Factors, Diagnostic Tests, Routine veterinary, Horse Diseases diagnosis, Inflammation veterinary, Serum Amyloid A Protein analysis
- Abstract
Background: An in-clinic assay for equine serum amyloid A (SAA) analysis, Equinostic EVA1, was evaluated for use in a clinical setting. Stability of SAA in serum samples was determined., Methods: Intra- and inter- assay variation of the in-clinic method was determined. The in-clinic method (EVA1) results were compared to a reference method (Eiken LZ SAA) with 62 patient samples. For samples with SAA concentrations within the assay range of EVA1 (10-270 mg/L), differences between the methods were evaluated in a difference plot. Linearity under dilution was evaluated in two samples. Stability of SAA in three serum pools stored at 4 degrees C and approximately 22 degrees C was evaluated with the reference method day 0, 1, 2, 4, 7, 17 and analysed with a two-way ANOVA., Results: The imprecision (coefficient of variation, CV) for the in-clinic method was acceptable at higher SAA concentrations with CV values of 7,3-12%, but poor at low SAA concentrations with CV values of 27% and 37% for intra- and inter-assay variation respectively. Recovery after dilution was 50-138%. The in-clinic assay and the reference method identified equally well horses with low (<10 mg/L) and high (>270 mg/L) SAA concentrations. Within the assay range of the in-clinic method, 10-270 mg/L, the difference between the two methods was slightly higher than could be explained by the inherent imprecision of the assays. There were no significant changes of serum SAA concentrations during storage., Conclusions: The in-clinic assay identified horses with SAA concentrations of <10 mg/L and >270 mg/L in a similar way as the reference method, and provided an estimate of the SAA concentration in the range of 10-270 mg/L. The imprecision of the in-clinic method was acceptable at high SAA concentrations but not at low concentrations. Dilution of samples gave inconsistent results. SAA was stable both at room temperature and refrigerated, and thus samples may be stored before analysis with the reference method.
- Published
- 2010
- Full Text
- View/download PDF
36. Atypical mitoses: morphology and classification.
- Author
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Tvedten H
- Subjects
- Animal Diseases diagnosis, Animals, Histological Techniques, Neoplasms classification, Neoplasms diagnosis, Animal Diseases classification, Mitosis physiology, Neoplasms veterinary
- Published
- 2009
- Full Text
- View/download PDF
37. Validation of the Sysmex XT-2000iV hematology system for dogs, cats, and horses. I. Erythrocytes, platelets, and total leukocyte counts.
- Author
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Lilliehöök I and Tvedten H
- Subjects
- Animals, Blood Cell Count instrumentation, Cats blood, Dogs blood, Horses blood, Reproducibility of Results, Sensitivity and Specificity, Species Specificity, Blood Cell Count veterinary, Blood Platelets cytology, Erythrocytes cytology, Leukocytes cytology
- Abstract
Background: The Sysmex XT-2000iV is a laser-based, flow cytometric hematology system that has been introduced for use in large and referral veterinary laboratories., Objective: The purpose of this study was to validate the Sysmex XT-2000iV for counting erythrocytes, reticulocytes, platelets, and total leukocytes in blood from ill dogs, cats, and horses., Methods: Blood samples from diseased animals (133 dogs, 65 cats, and 73 horses) were analyzed with the Sysmex XT-2000iV and the CELL-DYN 3500. Manual reticulocyte counts were done on an additional 98 canine and 14 feline samples and manual platelet counts were done on an additional 73 feline and 55 canine samples, and compared with automated Sysmex results., Results: Hemoglobin concentration, RBC counts, and total WBC counts on the Sysmex were highly correlated with those from the CELL-DYN (r>/=0.98). Systematic differences occurred for MCV and HCT. MCHC was poorly correlated in all species (r=0.33-0.67). The Sysmex impedance platelet count in dogs was highly correlated with both the impedance count from the CELL-DYN (r=0.99) and the optical platelet count from the Sysmex (r=0.98). The Sysmex optical platelet count included large platelets, such that in samples from cats, the results agreed better with manual platelet counts than with impedance platelet counts on the Sysmex. Canine reticulocyte counts on the Sysmex correlated well (r=0.90) with manual reticulocyte counts. Feline reticulocyte counts on the Sysmex correlated well with aggregate (r=0.86) but not punctate (r=0.50) reticulocyte counts., Conclusion: The Sysmex XT-2000iV performed as well as the CELL-DYN on blood samples from dogs, cats, and horses with a variety of hematologic abnormalities. In addition, the Sysmex detected large platelets and provided accurate reticulocyte counts.
- Published
- 2009
- Full Text
- View/download PDF
38. Validation of the Sysmex XT-2000iV hematology system for dogs, cats, and horses. II. Differential leukocyte counts.
- Author
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Lilliehöök I and Tvedten H
- Subjects
- Animals, Leukocyte Count instrumentation, Lymphocyte Subsets, Reproducibility of Results, Sensitivity and Specificity, Species Specificity, Cats blood, Dogs blood, Horses blood, Leukocyte Count veterinary
- Abstract
Background: The Sysmex XT-2000iV is a laser-based, flow cytometric hematology system that stains nucleic acids in leukocytes with a fluorescent dye. A 4-part differential is obtained using side fluorescence light and laser side scatter., Objective: The purpose of this study was to validate the Sysmex XT-2000iV for determining differential leukocyte counts in blood from ill dogs, cats, and horses., Methods: Blood samples from diseased animals (133 dogs, 65 cats, and 73 horses) were analyzed with the Sysmex XT-2000iV (Auto-diff) and the CELL-DYN 3500. Manual differentials were obtained by counting 100 leukocytes in Wright-stained blood smears., Results: Leukocyte populations in the Sysmex DIFF scattergram were usually well separated in equine samples, but were not as well separated in canine and feline samples. Correlation among the Sysmex XT-2000iV, CELL-DYN 3500, and manual counts was excellent for neutrophil counts (r >or=.97) and good for lymphocyte counts (r >or=.87) for all three species. Systematic differences between the 3 methods were seen for lymphocyte and monocyte counts. The Sysmex reported incomplete differential counts on 18% of feline, 13% of canine, and 3% of equine samples, often when a marked left shift (>10% bands) and/or toxic neutrophils were present. Eosinophils were readily identified in cytograms from all 3 species. Neither the Sysmex nor the CELL-DYN detected basophils in the 7 dogs and 5 cats with basophilia., Conclusions: The Sysmex XT-2000iV automated differential leukocyte count performed well with most samples from diseased dogs, cats, and horses. Basophils were not detected. Immature neutrophils or prominent toxic changes often induced errors in samples from cats and dogs.
- Published
- 2009
- Full Text
- View/download PDF
39. Plateletcrit is superior to platelet count for assessing platelet status in Cavalier King Charles Spaniels.
- Author
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Tvedten H, Lilliehöök I, Hillström A, and Häggström J
- Subjects
- Animals, Blood Platelet Disorders genetics, Diagnostic Tests, Routine veterinary, Dog Diseases blood, Dogs, Predictive Value of Tests, Blood Platelet Disorders veterinary, Dog Diseases genetics, Platelet Count veterinary
- Abstract
Background: Many Cavalier King Charles Spaniel (CKCS) dogs are affected by an autosomal recessive dysplasia of platelets resulting in fewer but larger platelets. The IDEXX Vet Autoread (QBC) hematology analyzer directly measures the relative volume of platelets in a blood sample (plateletcrit). We hypothesized that CKCS both with and without hereditary macrothrombocytosis would have a normal plateletcrit and that the QBC results would better identify the total circulating volume of platelets in CKSC than methods directly enumerating platelet numbers., Objectives: The major purpose of this study was to compare the QBC platelet results with platelet counts from other automated and manual methods for evaluating platelet status in CKCS dogs., Methods: Platelet counts were determined in fresh EDTA blood from 27 adult CKCS dogs using the QBC, Sysmex XT-2000iV (optical and impedance), CELL-DYN 3500, blood smear estimate, and manual methods. Sysmex optical platelet counts were reanalyzed following gating to determine the number and percentage of normal- and large-sized platelets in each blood sample., Results: None of the 27 CKCS dogs had thrombocytopenia (defined as <164 x 10(9) platelets/L) based on the QBC platelet count. Fourteen (52%) to 18 (66%) of the dogs had thrombocytopenia with other methods. The percentage of large platelets, as determined by regating the Sysmex optical platelet counts, ranged from 1% to 75%, in a gradual continuum., Conclusions: The QBC may be the best analyzer for assessing clinically relevant thrombocytopenia in CKCS dogs, because its platelet count is based on the plateletcrit, a measurement of platelet mass.
- Published
- 2008
- Full Text
- View/download PDF
40. Hypersegmentation of equine neutrophils.
- Author
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Tvedten H and Riihimäki M
- Subjects
- Animals, Horses physiology, Neutrophils cytology
- Published
- 2007
- Full Text
- View/download PDF
41. Differential leukocyte counts determined in chicken blood using the Cell-Dyn 3500.
- Author
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Lilliehöök I, Wall H, Tauson R, and Tvedten H
- Subjects
- Animals, Autoanalysis instrumentation, Autoanalysis methods, Autoanalysis standards, Female, Granulocytes cytology, Hematology instrumentation, Hematology methods, Hematology standards, Leukocyte Count instrumentation, Leukocyte Count standards, Reproducibility of Results, Sensitivity and Specificity, Autoanalysis veterinary, Chickens blood, Leukocyte Count veterinary
- Abstract
Background: Automated hematology instruments commonly are used for mammalian blood analysis, but there is a lack of accurate automated methods available for avian leukocyte analysis., Objective: The aim of this study was to validate differential leukocyte counts in blood from chickens using the Cell-Dyn 3500 hematology system and avian-specific software., Methods: Blood samples were collected in lithium-heparin tubes from 2 groups (n = 84 and n = 139) of laying hens. Manual 200-cell differential counts were done on routinely-stained blood smears, and manual total granulocyte counts (heterophils and eosinophils) were done using an eosinophil stain in a counting chamber. Automated differential counts were done using VET 2.3, a research and development version of avian-specific software for the Cell-Dyn 3500. Results were analyzed using Pearson's correlation and difference plots., Results: Automated granulocyte counts from the Cell-Dyn were in good agreement with manual granulocyte counts (r = 0.93 and 0.80 for the 2 study groups). No correlation was found between automated and manual lymphocyte counts. Correlation coefficients for monocyte counts were 0.70 and 0.43., Conclusion: Automated leukocyte results from the Cell-Dyn using VET 2.3 software were not fully accurate. Total granulocyte counts may be of clinical usefulness, but results obtained for other parameters were unreliable.
- Published
- 2004
- Full Text
- View/download PDF
42. Investigation of hypereosinophilia and potential treatments.
- Author
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Lilliehöök I and Tvedten H
- Subjects
- Animals, Cat Diseases blood, Cats, Dog Diseases blood, Dogs, Hypereosinophilic Syndrome diagnosis, Hypereosinophilic Syndrome therapy, Leukocyte Count veterinary, Cat Diseases diagnosis, Cat Diseases therapy, Dog Diseases diagnosis, Dog Diseases therapy, Eosinophils, Hypereosinophilic Syndrome veterinary
- Abstract
Hypereosinophilia is excessive eosinophilia and has been defined in dogs and cats as eosinophils greater than 5 x 10(9)/L (> 5000/microL). Canine breeds with a predisposition to higher eosinophil counts or certain eosinophilic diseases include the Rottweiler, German Shepard, Siberian Husky, Alaskan Malamute, and Cavalier King Charles Spaniel. Two of the more common causes of canine hypereosinophilia are pulmonary infiltrates with eosinophils (PIE) and gastrointestinal disease. The highest eosinophil counts are expected in dogs with pneumonia or PIE. The most common cause of eosinophilia in cats is flea allergy. The greatest eosinophilia occurs in cats with flea allergy, feline asthma, and eosinophilic granuloma. Innovative recent treatments for human patients with asthma have been successful in reducing eosinophil numbers but have had a confusing and disappointing lack of reducing symptoms. The role of eosinophils in many eosinophilic diseases remains a mystery.
- Published
- 2003
- Full Text
- View/download PDF
43. Vortex mixing of feline blood to disaggregate platelet clumps.
- Author
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Tvedten H and Korcal D
- Abstract
Platelet clumping is a common cause of erroneous platelet counts in cats. Mixing of blood with a vortex mixer was evaluated as a method to disaggregate platelet clumps in feline blood and thus obtain accurate platelet counts. Whole blood samples from 42 cats with platelet clumping and 10 control cats without platelet clumping were mixed for 1 minute at the maximal setting using a standard vortex mixer. Blood smears (for subjective assessment of the type and amount of platelet clumping), platelet counts, and total leukocyte counts were evaluated before and after mixing. Vortex treatment of blood samples with platelet clumps caused an increased platelet count in all but 1 sample. Although most samples had strong increases in platelet counts after mixing, only a minority of samples (5 of 42) appeared to have all platelet clumps dispersed. Of 39 feline blood samples with platelet counts initially <200X10(9) cells/L, 23 counts increased to >200X10(9) cells/L and 34 counts increased to >100X10(9) cells/L. Overall, mixing gave inconsistent and partial improvement in platelet counts. Total leukocyte counts were not significantly affected by vortex mixing. Vortex mixing of 10 feline blood samples without platelet clumping had no consistent effect on platelet or WBC counts. In conclusion, vortex mixing of feline blood does not appear to be a consistent means of correcting the problem of feline platelet clumping.
- Published
- 2001
- Full Text
- View/download PDF
44. Validation of the Coulter AcT Diff hematology analyzer for analysis of blood of common domestic animals.
- Author
-
Dawson H, Hoff B, Grift E, Tvedten H, and Shoukri M
- Abstract
The objective of this study was to compare and assess the agreement between the Coulter AcT Diff hematology analyzer (CAD) and the Bayer Technicon H1 (H1) using blood samples from 391 animals of 4 species. The H1 has been used in veterinary laboratories for many years. Recently, Coulter modified the CAD and added veterinary software for hematologic analysis of feline, canine, and equine samples. A comparison of hemograms from dogs, cats, horses, and cattle was made using EDTA-anticoagulated blood samples. Both instruments were calibrated using human blood products. Performance characteristics were excellent for most values. The exceptions were MCV in canine samples (concordance correlation of.710), platelet counts for feline and equine samples (.258 and.740, respectively), feline and bovine WBC counts (.863 and.857, respectively), and bovine hemoglobin (.876).
- Published
- 2000
- Full Text
- View/download PDF
45. Mixed venous and arterial blood in bovine coccygeal vessel samples for blood gas analysis.
- Author
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Tvedten H, Kopcia M, and Haines C
- Abstract
Bovine coccygeal (median, caudal) vessel samples are not always venous in origin but may be arterial or a mixture of venous and arterial blood. Results of blood gas analysis of blood samples collected from 39 cows were consistent with typical venous, mixed venous-arterial, or typical arterial blood. This observation was made while establishing reference intervals for a new blood gas instrument. The pO2 and the oxygen saturation of hemoglobin (S02) were most reflective of the amount of arterial blood contamination in the sample, but pCO2 and pH also were altered. The pO2 values ranged from 21 to 127 mm Hg, and SO2 ranged from 35% to 100%. The mean pCO2 in venous type samples was 46.3 mm Hg compared to 36.7 mm Hg in arterial type samples. The mean pH in venous type samples was 7.42 compared to 7.47 in arterial type samples.
- Published
- 2000
- Full Text
- View/download PDF
46. Radiographic, biomechanical, and pathologic effects of hemoglobin glutamer-200 in dogs undergoing cemented total hip arthroplasty.
- Author
-
Braden TD, Tvedten HW, DeCamp CE, Turner TM, Hughes GS, and Rentko VT
- Subjects
- Acetabulum drug effects, Animals, Arthroplasty, Replacement, Hip methods, Biomechanical Phenomena, Blood Chemical Analysis, Bone Cements, Cattle, Dogs, Female, Femur diagnostic imaging, Femur drug effects, Gait, Hemoglobins, Male, Radiography, Arthroplasty, Replacement, Hip veterinary, Blood Substitutes pharmacology
- Abstract
Objective: To determine whether use of hemoglobin glutamer-200 (bovine) as a partial blood volume replacement in dogs undergoing cemented total hip replacement caused any deleterious effects on the bone-cement or cement-prosthesis interface, exerted any deleterious effects on body organs, or caused any complications during the anesthetic, immediate recovery, or long-term recovery period., Animals: 9 adult dogs., Methods: Dogs were anesthetized, and 15% of the blood volume was removed. Simultaneously, lactated Ringer's solution was infused, and 6 dogs were given hemoglobin glutamer (1 g/kg of body weight, IV). Unilateral total hip replacement was performed. Limb use was assessed visually, and force-plate and radiographic evaluations were performed before, and 8 weeks after, surgery. Eight weeks after surgery, dogs were euthanatized, necropsies were performed, and prosthetic component pullout forces were determined., Results: There were no significant differences between treated and control dogs in regard to biomechanical (visual assessment of gait, force-plate analysis, femoral and acetabular component pullout forces) and pathologic evaluations (physical examination, CBC, serum biochemical analyses, necropsy, and histologic evaluations). Radiographic signs of loosening of the femoral component were seen in 4 dogs treated with hemoglobin glutamer., Conclusions and Clinical Relevance: Administration of hemoglobin glutamer as a blood substitute did not appear to have any deleterious effects in dogs undergoing total hip arthroplasty. The radiographic findings, which were discordant with the biomechanical results, merit further investigation.
- Published
- 1999
47. Chronic granulocytic leukemia in a dog.
- Author
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Fine DM and Tvedten HW
- Subjects
- Alopecia chemically induced, Alopecia veterinary, Animals, Antineoplastic Agents adverse effects, Antineoplastic Agents therapeutic use, Biopsy veterinary, Bone Marrow pathology, Dog Diseases chemically induced, Dog Diseases drug therapy, Dogs, Female, Hydroxyurea adverse effects, Hydroxyurea therapeutic use, Leukemia, Myelogenous, Chronic, BCR-ABL Positive diagnosis, Leukemia, Myelogenous, Chronic, BCR-ABL Positive drug therapy, Leukocyte Count veterinary, Liver pathology, Lymph Nodes pathology, Dog Diseases diagnosis, Leukemia, Myelogenous, Chronic, BCR-ABL Positive veterinary
- Abstract
A 4-year-old spayed dog had a recent history of increased WBC count and surgery for pyometra. Two weeks after surgery, WBC count was 57,640 cells/microliter; neutrophilia and immature myelocytic cells were detected. Histologic examination of liver and lymph node biopsy specimens revealed active granulopoiesis. Immature granulocytes that stained with chloroacetate esterase were evident. Bone marrow was excessively cellular and contained numerous granulocytes and blast cells. A diagnosis of chronic granulocytic leukemia was made on the basis of test results. Treatment with hydroxyurea returned the WBC count to reference range within 2 months. Mean survival time for dogs with chronic granulocytic leukemia is approximately 1 year; the dog of this report has remained healthy for more than 2 years. Chronic granulocytic leukemia is a rare neoplastic disease that must be differentiated from leukemoid inflammatory reactions. Although commonly described as a diagnosis determined by exclusion, diagnosis of chronic granulocytic leukemia should be made on the basis of specific criteria.
- Published
- 1999
48. Pseudohypophosphatemia in two dogs with immune-mediated hemolytic anemia.
- Author
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Harkin KR, Braselton WE, and Tvedten H
- Subjects
- Anemia, Hemolytic blood, Anemia, Hemolytic immunology, Animals, Artifacts, Calcium blood, Diagnosis, Differential, Dogs, Hypophosphatemia blood, Hypophosphatemia diagnosis, Magnesium blood, Male, Phosphates blood, Potassium blood, Risk Factors, Sodium blood, Anemia, Hemolytic veterinary, Dog Diseases, Hypophosphatemia veterinary
- Published
- 1998
- Full Text
- View/download PDF
49. What is your diagnosis? Synovial fluid from a dog.
- Author
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Bolliger AP, DeCamp C, and Tvedten H
- Published
- 1994
- Full Text
- View/download PDF
50. Advanced hematology analyzers interpretation of results.
- Author
-
Tvedten H
- Published
- 1993
- Full Text
- View/download PDF
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