105 results on '"Tutino, V."'
Search Results
2. Trends in adherence to the Mediterranean diet in South Italy: A cross sectional study
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Veronese, N., Notarnicola, M., Cisternino, A. M., Inguaggiato, R., Guerra, V., Reddavide, R., Donghia, R., Rotolo, O., Zinzi, I., Leandro, G., Tutino, V., Misciagna, G., Caruso, M. G., Correale, M., Giampiero, D. M., Anna, M., Palma, I., Marisa, N., Chiloiro, M., Osvaldo, B., Vittorio, P., D'Attoma, B., the MICOL study group, Veronese, N., Notarnicola, M., Cisternino, A.M., Inguaggiato, R., Guerra, V., Reddavide, R., Donghia, R., Rotolo, O., Zinzi, I., Leandro, G., Tutino, V., Misciagna, G., Caruso, M.G., Correale, M., Giampiero, D.M., Anna, M., Palma, I., Marisa, N., Chiloiro, M., Osvaldo, B., Vittorio, P., D'Attoma, B., and the MICOL study group
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Adult ,Male ,Health Knowledge, Attitudes, Practice ,medicine.medical_specialty ,Time Factors ,Mediterranean diet ,Epidemiology ,Cross-sectional study ,Endocrinology, Diabetes and Metabolism ,Health Behavior ,Nutritional Status ,Medicine (miscellaneous) ,030209 endocrinology & metabolism ,Younger people ,030204 cardiovascular system & hematology ,Diet, Mediterranean ,Recommended Dietary Allowances ,Diet Surveys ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Humans ,Aged ,Nutrition and Dietetics ,business.industry ,Age Factors ,Feeding Behavior ,Middle Aged ,Large cohort ,Cross-Sectional Studies ,Italy ,Female ,Diet, Healthy ,Cardiology and Cardiovascular Medicine ,business ,Nutritive Value ,Olive oil ,Trend ,Demography - Abstract
Background and aims: Increasing literature data show that adherence to the Mediterranean diet is undergoing profound changes in recent years, albeit with marked differences across nations. In Italy, one of the cradles of the Mediterranean diet, the literature regarding the trend for Mediterranean diet adherence is conflicting. Thus, we aimed to explore the trends of adherence to the Mediterranean diet in a large cohort of participants living in South Italy, over 20 years from 1985–86 to 2005–06. Methods and results: Cross-sectional study with two evaluations, one made in 1985–86 and another in 2005–06; all participants were adults aged 30–70 years of age. The adherence to the Mediterranean diet was evaluated using the score proposed by Panagiotakos et al. This score features values ranging from 0 to 55, higher scores reflecting a greater adherence. The data are reported by age (30–49 vs. 50–69 years). Overall, 2451 subjects were included in 1985–86 and 2375 in 2005–06. A significant reduction was observed in the adherence to the Mediterranean diet (age 30–49 years: 31.82 ± 4.18 in 1985–86 vs. 29.20 ± 4.48 in 2005–06, reduction by 8.2%, p < 0.0001; age 50–69: 32.20 ± 4.09 in 1985–86 vs.30.15 ± 4.27 in 2005–06, reduction by 6.3%, p < 0.0001). Among all these items, the most dramatic change was observed for olive oil consumption, that decreased by 2.35 points in younger and 0.89 in older people. Conclusion: The adherence to the Mediterranean diet decreased from 1985–86 to 2005–06 in South Italy, particularly in younger people, above all due to a decreased olive oil consumption. © 2019 The Italian Society of Diabetology, the Italian Society for the Study of Atherosclerosis, the Italian Society of Human Nutrition, and the Department of Clinical Medicine and Surgery, Federico II University
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- 2020
3. Dietary inflammatory index and mortality: a cohort longitudinal study in a Mediterranean area
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Veronese, N., Cisternino, A. M., Shivappa, N., Hebert, J. R., Notarnicola, M., Reddavide, R., Inguaggiato, R., Guerra, V., Logroscino, A., Rotolo, O., Chiloiro, M., Leandro, G., De Leonardis, G., Tutino, V., Misciagna, G., Fontana, L., Caruso, M. G., Giampiero, D. M., Marisa, N., Osvaldo, B., Benedetta, D., Vittorio, P., The MICOL group, Veronese, N., Cisternino, A.M., Shivappa, N., Hebert, J.R., Notarnicola, M., Reddavide, R., Inguaggiato, R., Guerra, V., Logroscino, A., Rotolo, O., Chiloiro, M., Leandro, G., De Leonardis, G., Tutino, V., Misciagna, G., Fontana, L., Caruso, M.G., Giampiero, D.M., Marisa, N., Osvaldo, B., Benedetta, D., Vittorio, P., and The MICOL group
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Male ,0301 basic medicine ,medicine.medical_specialty ,Longitudinal study ,Medicine (miscellaneous) ,030209 endocrinology & metabolism ,Disease ,Diet Surveys ,03 medical and health sciences ,cancer ,cardiovascular disease ,dietary inflammatory index ,mortality ,0302 clinical medicine ,Cause of Death ,Neoplasms ,Internal medicine ,medicine ,Humans ,Longitudinal Studies ,Aged ,Proportional Hazards Models ,Inflammation ,030109 nutrition & dietetics ,Nutrition and Dietetics ,Mediterranean Region ,business.industry ,Hazard ratio ,Confounding ,Middle Aged ,Confidence interval ,Increased risk ,Cardiovascular Diseases ,Cohort ,Regression Analysis ,Mediterranean area ,Female ,sense organs ,Diet, Healthy ,business - Abstract
Background: Higher Dietary Inflammatory Index (DII®) scores are associated with increased morbidity and mortality. However, little is known about the effects of DII on mortality in Mediterranean countries. Therefore, in the present study, we aimed to investigate the potential association between DII scores and overall, cancer and cardiovascular disease (CVD) mortality in people living in a Mediterranean area. Methods: DII scores were calculated using a validated food-frequency questionnaire. DII scores were then categorised into tertiles. Mortality was ascertained via death certificates. The association between DII scores with overall and cause-specific mortality was assessed via a multivariable Cox's regression analysis and reported as hazard ratios (HRs) with their 95% confidence intervals (CIs). Results: The study included 1565 participants (mean age65.5years; females44.7%). After a median follow-up of 12years (2005–2017), 366 (23.4%) participants died. After adjusting for 17 potential confounders, people with higher DII scores had an increased risk of death compared to those in the lowest (most anti-inflammatory) tertile (HR=1.38; 95% CI=1.04–1.82 for the second tertile; HR=1.38; 95% CI=1.03–1.86 for the third tertile). Each 1 SD increase in DII score increased the risk of death by 13%. No association was found between DII scores and cancer or CVD death when considered separately. Conclusions: Higher DII scores were associated with a significantly higher mortality risk, whereas the association with cause-specific mortality was less clear. These findings highlight the potential importance of diet in modulating inflammation and preventing death. © 2019 The British Dietetic Association Ltd.
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- 2019
4. Initial evaluation of 2D and 3D simulated high-speed 1000 fps vascular contrast-flow image sequences using computational fluid dynamics (CFD)
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Shields, A., Williams, K., Veeturi, S.S., Tutino, V., Ionita, C., Bednarek, D.R., and Rudin, S.
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Article - Abstract
Digital subtraction angiography (DSA) remains the clinical standard for detailed visualization of the neurovasculature due to its high-spatial resolution; however, detailed blood-flow quantification is impaired by its low-temporal resolution. Advances in photon-counting detector technology have led us to develop High-Speed Angiography (HSA), where x-ray images are acquired at 1000 fps for more accurate visualization and quantification of blood flow. We have implemented a physics-based optical flow method to extract such information from HSA, but validation of the angiography-derived velocity distributions is not straightforward. Computational fluid dynamics (CFD) is widely regarded as the benchmark for hemodynamic analysis, as it provides a multitude of quantitative flow parameters throughout the volume of interest. However, there are several limitations with this method related to over-simplification of boundary conditions and suboptimal meshing (spatial resolution), that make CFD simulation results an inexact criterion for validation. To overcome this issue for HSA validation, CFD was used to generate both simulated high-speed angiograms and the corresponding ground-truth 3D flow fields to better understand the relationship between the 3D volumetric-flow distribution and the 2D projected-flow distribution as is obtained with angiography, and the subsequent 2D approximation of flow velocity. Several geometries were investigated, ranging from simple pipe models to complex patient-specific aneurysms. Simulated datasets were analyzed with the optical flow algorithm, and the effects of flow divergence, quantum mottle, and intensity gradient on the calculation were evaluated. From these simulations, we can evaluate whether flow fields reconstructed from HSA are representative of significant flow patterns in the 3D vasculature.
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- 2022
5. P-272 Estrogen receptors influence the epithelial-to-mesenchymal transition of pancreatic cancer cell lines
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Licitra, F., primary, Giovannelli, P., additional, Di Donato, M., additional, Tutino, V., additional, Migliaccio, A., additional, and Castoria, G., additional
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- 2022
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6. Learning Curve for Flow Diversion of Posterior Circulation Aneurysms: A Long-Term International Multicenter Cohort Study.
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Adeeb, N., Dibas, M., Griessenauer, C. J., Cuellar, H. H., Salem, M. M., Xiang, S., Enriquez-Marulanda, A., Hong, T., Zhang, H., Taussky, P., Grandhi, R., Waqas, M., Aldine, A. S., Tutino, V. M., Aslan, A., Siddiqui, A. H., Levy, E. I., Ogilvy, C. S., Thomas, A. J., and Ulfert, C.
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- 2022
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7. Integrated Small Dense Low-density Lipoprotein Profile in Cardiovascular Disease and Cancer: A longitudinal study
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Notarnicola, M., Nunzio, V. D. E., Tutino, V., Veronese, N., Guerra, V., Osella, A. R., Caruso, M. G., MICOL GROUP, Notarnicola, M., Nunzio, V.D.E., Tutino, V., Veronese, N., Guerra, V., Osella, A.R., Caruso, M.G., and MICOL GROUP
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Adult ,Male ,Cancer Research ,medicine.medical_specialty ,Longitudinal study ,Small dense ldl ,Disease ,Age and sex ,Gastroenterology ,chemistry.chemical_compound ,Risk Factors ,Internal medicine ,Neoplasms ,Medicine ,Humans ,In patient ,Longitudinal Studies ,Aged ,business.industry ,Cancer ,sdLDL, cardiovascular disease, cancer ,General Medicine ,Middle Aged ,medicine.disease ,Prognosis ,Lipoproteins, LDL ,Oncology ,chemistry ,Cardiovascular Diseases ,Low-density lipoprotein ,Case-Control Studies ,Cohort ,Female ,business ,Biomarkers ,Follow-Up Studies - Abstract
Background/Aim: Low-density lipoproteins (LDL) are a heterogeneous class of particles that differ in size and density from each other. Small dense LDL (sdLDL) particles are considered more atherogenic than larger particles. The aim of the study was to evaluate serum levels of sdLDL in patients who died from cardiovascular diseases (CVD) or cancer in a cohort of patients followed up in the De Bellis Research Hospital for 20 years. Patients and Methods: A total of 75 participants who died of cancer and 87 who died of CVD were enrolled and they were matched for age and sex with 135 healthy controls, i.e. without CVD or cancer and are still alive. Results: Patients who died from cancer had the highest value of LDL IV subfraction (0.25±1.16), followed by those who died from CVD (0.17±0.96). Conclusion: The integrated profile of sdLDL between CVD and cancer suggests that therapeutic modulation of sdLDL may be associated with a risk reduction for these diseases. © 2019 International Institute of Anticancer Research. All rights reserved.
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- 2019
8. E-230 Comparison of PED and FRED flow diverters for posterior circulation aneurysms: a propensity-score matched cohort study
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Griessenauer, C, primary, Enriquez-Marulanda, A, additional, Xiang, S, additional, Hong, T, additional, Zhang, H, additional, Taussky, P, additional, Grandhi, R, additional, Waqas, M, additional, Tutino, V, additional, Siddiqui, A, additional, Levy, E, additional, Ogilvy, C, additional, Thomas, A, additional, Ulfert, C, additional, Möhlenbruch, M, additional, Renieri, L, additional, Limbucci, N, additional, Parra-Fariñas, C, additional, Burkhardt, J, additional, Kan, P, additional, Rinaldo, L, additional, Lanzino, G, additional, Brinjikji, W, additional, Müller-Thies-Broussalis, E, additional, Killer-Oberpfalzer, M, additional, Islak, C, additional, Kocer, N, additional, Sonnberger, M, additional, Engelhorn, T, additional, Ghuman, M, additional, Yang, V, additional, Salehani, A, additional, Harrigan, M, additional, Radovanovic, I, additional, and Dmytriw, A, additional
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- 2020
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9. Coffee Intake and Liver Steatosis: A Population Study in a Mediterranean Area
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Veronese, N., Notarnicola, M., Cisternino, A. M., Reddavide, R., Inguaggiato, R., Guerra, V., Rotolo, O., Zinzi, I., Leandro, G., Correale, M., Tutino, V., Misciagna, G., Osella, A. R., Bonfiglio, C., Giannelli, G., Caruso, M. G., The MICOL Group, Veronese, N., Notarnicola, M., Cisternino, A.M., Reddavide, R., Inguaggiato, R., Guerra, V., Rotolo, O., Zinzi, I., Leandro, G., Correale, M., Tutino, V., Misciagna, G., Osella, A.R., Bonfiglio, C., Giannelli, G., Caruso, M.G., and The MICOL Group
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Male ,Cross-sectional study ,Blood Pressure ,Gastroenterology ,Body Mass Index ,chemistry.chemical_compound ,0302 clinical medicine ,Non-alcoholic Fatty Liver Disease ,Surveys and Questionnaires ,Prevalence ,caffeine ,Aged, 80 and over ,Nutrition and Dietetics ,ultrasound ,Mediterranean Region ,Fatty liver ,Middle Aged ,3. Good health ,Italy ,030220 oncology & carcinogenesis ,Population study ,030211 gastroenterology & hepatology ,epidemiology ,Female ,Waist Circumference ,Caffeine ,lcsh:Nutrition. Foods and food supply ,Fatty Liver, Alcoholic ,Adult ,medicine.medical_specialty ,Alcohol Drinking ,coffee ,lcsh:TX341-641 ,Article ,03 medical and health sciences ,Internal medicine ,medicine ,Humans ,fatty liver ,Aged ,business.industry ,Odds ratio ,medicine.disease ,Cross-Sectional Studies ,Logistic Models ,chemistry ,Alcoholic fatty liver ,Steatosis ,business ,Body mass index ,Food Science - Abstract
Coffee drinking seems to have several beneficial effects on health outcomes. However, the effect on hepatic steatosis, depending on a high alcohol consumption (AFLD, alcoholic fatty liver disease) or on metabolic factors (non-alcoholic fatty liver disease, NAFLD), is still equivocal. Thus, we aimed to explore the potential association between coffee consumption and the presence and severity of hepatic steatosis in people with NAFLD or AFLD. In this cross-sectional study, coffee drinking was recorded using a semi-quantitative food frequency questionnaire, and categorized as yes vs. no and as 0, 1, 2, ≥3. The degree of fatty liver was assessed through a standardized ultrasound examination (score 0 to 6, with higher values reflecting higher severity). Liver steatosis was classified as NAFLD or AFLD on daily alcohol intake >30 g/day for men and >20 g/day for women. This study included 2819 middle-aged participants, the great majority were coffee drinkers (86.1%). After adjusting for 12 potential confounders, drinking coffee was not associated with decreased odds for NAFLD (n = 916) (odds ratio, OR = 0.93, 95% confidence intervals, CI: 0.72-1.20) or AFLD (n = 276) (OR = 1.20, 95% CI: 0.66-2.0). The consumption of coffee (categorized as yes vs. no), or an increased consumption of coffee were not associated with the presence of mild, moderate or severe liver steatosis in either NAFLD or AFLD. In conclusion, coffee intake was not associated with any lower odds of hepatic steatosis in either non-alcoholic or alcoholic forms in this large cohort of South Italian individuals. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.
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- 2018
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10. Nutrition and lipidomic profile in colorectal cancers
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Notarnicola, M., Caruso, M. G., Tutino, V., De Nunzio, V., Gigante, I., De Leonardis, G., Veronese, N., Rotolo, O., Reddavide, R., Stasi, E., Miraglia, C., Nouvenne, A., Meschi, T., De’ Angelis, G. L., Di Mario, F., Leandro, G., Notarnicola, M., Caruso, M.G., Tutino, V., De Nunzio, V., Gigante, I., De Leonardis, G., Veronese, N., Rotolo, O., Reddavide, R., Stasi, E., Miraglia, C., Nouvenne, A., Meschi, T., De’ Angelis, G.L., Di Mario, F., and Leandro, G.
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nutrition, colorectal cancer, lipidomic analysis, fatty acids, lipoproteins - Abstract
Background: Adherence to a healthy diet has been reported to be essential for the primary prevention of colorectal cancer, through a reduction of tissue inflammation, a low concentration of circulating lipoproteins and lower levels of serum cholesterol. Since an altered expression of the fatty acids pattern has been demonstrated to be a crucial event in colorectal carcinogenesis, lipidomic analysis is considered able to identify early diagnostic and prognostic biomarkers of complex diseases such as colorectal cancer. Methods: cell membrane fatty acid profile and serum lipoproteins pattern were evaluated by gas chromatography and electrophoresis method respectively. Results: There is a close association between diet and lipidomic profile in colorectal cancer, both in pre-clinical and clinical studies. A modified serum lipoproteins pattern has been demonstrated to be predominant in intestinal tumors. Conclusions: The study of fatty acids profile in cell membrane and the evaluation of serum lipoproteins subfractions could be useful to have an integrate vision on the interactions between lipids and the pathogenesis of colorectal cancer and to understand the mechanisms of action and the consequences of these interactions on human health status. (www.actabiomedica.it). © Mattioli 1885.
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- 2018
11. Initial evaluation of 2D and 3D simulated high-speed 1000 fps vascular contrast-flow image sequences using computational fluid dynamics (CFD)
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Gimi, Barjor S., Krol, Andrzej, Shields, A., Williams, K., Veeturi, S. S., Tutino, V., Ionita, C., Bednarek, D. R., and Rudin, S.
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- 2022
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12. Increased Perviousness on CT for Acute Ischemic Stroke is Associated with Fibrin/Platelet-Rich Clots.
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Patel, T. R., Fricano, S., Waqas, M., Tso, M., Dmytriw, A. A., Mokin, M., Kolega, J., Tomaszewski, J., Levy, E. I., Davies, J. M., Snyder, K. V., Siddiqui, A. H., and Tutino, V. M.
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- 2021
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13. Dietary inflammatory index and mortality: a cohort longitudinal study in a Mediterranean area.
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Veronese, N., Cisternino, A. M., Shivappa, N., Hebert, J. R., Notarnicola, M., Reddavide, R., Inguaggiato, R., Guerra, V., Logroscino, A., Rotolo, O., Chiloiro, M., Leandro, G., De Leonardis, G., Tutino, V., Misciagna, G., Fontana, L., Caruso, M. G., Giampiero, De Michele, Marisa, Noviello, and Osvaldo, Burattini
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CARDIOVASCULAR disease related mortality ,MORTALITY risk factors ,CONFIDENCE intervals ,DIET ,INFLAMMATION ,LONGITUDINAL method ,MULTIVARIATE analysis ,QUESTIONNAIRES ,TUMORS ,PROPORTIONAL hazards models ,DESCRIPTIVE statistics - Abstract
Background: Higher Dietary Inflammatory Index (DII®) scores are associated with increased morbidity and mortality. However, little is known about the effects of DII on mortality in Mediterranean countries. Therefore, in the present study, we aimed to investigate the potential association between DII scores and overall, cancer and cardiovascular disease (CVD) mortality in people living in a Mediterranean area. Methods: DII scores were calculated using a validated food‐frequency questionnaire. DII scores were then categorised into tertiles. Mortality was ascertained via death certificates. The association between DII scores with overall and cause‐specific mortality was assessed via a multivariable Cox's regression analysis and reported as hazard ratios (HRs) with their 95% confidence intervals (CIs). Results: The study included 1565 participants (mean age 65.5 years; females 44.7%). After a median follow‐up of 12 years (2005–2017), 366 (23.4%) participants died. After adjusting for 17 potential confounders, people with higher DII scores had an increased risk of death compared to those in the lowest (most anti‐inflammatory) tertile (HR = 1.38; 95% CI = 1.04–1.82 for the second tertile; HR = 1.38; 95% CI = 1.03–1.86 for the third tertile). Each 1 SD increase in DII score increased the risk of death by 13%. No association was found between DII scores and cancer or CVD death when considered separately. Conclusions: Higher DII scores were associated with a significantly higher mortality risk, whereas the association with cause‐specific mortality was less clear. These findings highlight the potential importance of diet in modulating inflammation and preventing death. [ABSTRACT FROM AUTHOR]
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- 2020
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14. Anti Proliferative and Pro Apoptotic Effects of Flavonoid Quercetin Are Mediated by CB1 Receptor in Human Colon Cancer Cell Lines
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Refolo MG1, D'Alessandro R, Malerba N, Laezza C, Bifulco M, Messa C, Caruso MG, Notarnicola M, Tutino V., Refolo Maria, Grazia, D'Alessandro, Rosalba, Malerba, Natascia, Laezza, Chiara, Bifulco, Maurizio, Messa, Caterina, Caruso Maria, Gabriella, Notarnicola, Maria, and Tutino, Valeria
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Physiology ,Clinical Biochemistry ,Antineoplastic Agents ,Apoptosis ,Adenocarcinoma ,Antineoplastic Agent ,Receptor, Cannabinoid, CB1 ,Cell Movement ,CB1 receptor ,Wnt/?-catenin pathway ,colon cancer ,intracellular signaling ,quercetin ,Phytogenic ,Humans ,heterocyclic compounds ,Cannabinoid ,Antineoplastic Agents, Phytogenic ,Caco-2 Cells ,Cannabinoid Receptor Agonists ,Cell Proliferation ,Colonic Neoplasms ,Quercetin ,Signal Transduction ,Up-Regulation ,Medicine (all) ,Cell Biology ,Caco-2 Cell ,Colonic Neoplasm ,Cannabinoid Receptor Agonist ,food and beverages ,Apoptosi ,CB1 ,lipids (amino acids, peptides, and proteins) ,Human ,Receptor - Abstract
Quercetin, the major constituent of flavonoid and widely present in fruits and vegetables, is an attractive compound for cancer prevention due to its beneficial anti proliferative effects, showing a crucial role in the regulation of apoptosis and cell cycle signaling. In vitro studies have demonstrated that quercetin specifically influences colon cancer cell proliferation. Our experiments, using human colon adenocarcinoma cells, confirmed the anti proliferative effect of quercetin and gave intriguing new insight in to the knowledge of the mechanisms involved. We observed a significant increase in the expression of the endocannabinoids receptor (CB1-R) after quercetin treatment. CB1-R can be considered an estrogen responsive receptor and quercetin, having a structure similar to that of the estrogens, can interact with CB1-R leading to the regulation of cell growth. In order to clarify the contribution of the CB1-R to the quercetin action, we investigated some of the principal molecular pathways that are inhibited or activated by this natural compound. In particular we detected the inhibition of the major survival signals like the PI3K/Akt/mTOR and an induction of the pro apoptotic JNK/JUN pathways. Interestingly, the metabolism of ?-catenin was modified by flavonoid both directly and through activated CB1-R. In all the experiments done, the quercetin action has proven to be reinforced by anandamide (Met-F-AEA), a CB1-R agonist, and partially counteracted by SR141716, a CB1-R antagonist. These findings open new perspectives for anticancer therapeutic strategies. This article is protected by copyright. All rights reserved.
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- 2015
15. Tumor-Induced Alterations in Lipid Metabolism
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Notarnicola, M., primary, Tutino, V., additional, and Caruso, M.G., additional
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- 2014
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16. Reduced fructosamine-3-kinase activity and its mRNA in human distal colorectal carcinoma
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Notarnicola, M., primary, Caruso, Maria G., additional, Tutino, V., additional, Guerra, V., additional, Frisullo, S., additional, Altomare, D. F., additional, and Misciagna, G., additional
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- 2010
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17. Effects of anandamide on polyamine levels and cell growth in human colon cancer cells
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Linsalata, M., Notarnicola, M., Tutino, V., Maurizio Bifulco, Santoro, A., Laezza, C., Messa, C., Orlando, A., Caruso, M. G., Linsalata, M, Notarnicola, M, Tutino, V, Bifulco, M, Santoro, A, Laezza, C, Messa, C, Orlando, A, and Caruso, Mg.
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lipids (amino acids, peptides, and proteins) - Abstract
BACKGROUND: Anandamide (AEA) is an endogenous agonist for cannabinoid receptor CB1-R and seems to be involved in the control of cancer growth. Polyamines are compounds that play an important role in cell proliferation and differentiation. Our aim was to investigate the effect of AEA on the polyamine levels (putrescine, spermidine and spermine) and cell growth of three human colon cancer cell lines, positive for CB1-R. MATERIALS AND METHODS: After AEA treatment of DLD-1, HT-29 and SW620 cells, polyamine analysis was performed by high-performance liquid chromatography (HPLC) and cell growth was measured by 3-(4,5 di-methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test. CB1 gene expression was determined using reverse transcription and polymerase chain reaction (RT-PCR). RESULTS: AEA significantly reduced polyamine levels and cell proliferation dose-dependently when the tested cell lines were exposed for 24 h and 48 h. This inhibitory effect was mediated by CB1-R, since SR 1411716A, a selective CB-1 receptor antagonist, was able to entirely antagonize the effect of AEA. CB1-R mRNA levels were enhanced after AEA treatment in DLD-1 cells, whereas no induction was found in HT-29 and SW620 cells. CONCLUSION: It appears that mechanisms by which AEA may affect growth of colon cancer cells involve a decrease in cell proliferation rate by reducing the polyamine levels.
18. Down-Regulation of Cannabinoid Type 1 (CB1) Receptor and its Downstream Signaling Pathways in Metastatic Colorectal Cancer
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Nicola Veronese, Valentina De Nunzio, Maria Gabriella Caruso, Dionigi Lorusso, Isabella Gigante, Valeria Tutino, Gianluigi Giannelli, Maria Notarnicola, Tutino, V., Caruso, M.G., De Nunzio, V., Lorusso, D., Veronese, N., Gigante, I., Notarnicola, M., and Giannelli, G.
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0301 basic medicine ,Cancer Research ,Cannabinoid receptor ,Colorectal cancer ,colorectal cancer ,lcsh:RC254-282 ,Article ,Metastasis ,Malignant transformation ,03 medical and health sciences ,0302 clinical medicine ,Medicine ,metastasis ,endocannabinoid system ,Receptor ,business.industry ,Cancer ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,medicine.disease ,Primary tumor ,030104 developmental biology ,Oncology ,030220 oncology & carcinogenesis ,Cancer research ,Signal transduction ,business ,cannabinoid type 1 (CB1) receptor - Abstract
Changes in the regulation of endocannabinoid production, together with an altered expression of their receptors are hallmarks of cancer, including colorectal cancer (CRC). Although several studies have been conducted to understand the biological role of the CB1 receptor in cancer, little is known about its involvement in the metastatic process of CRC. The aim of this study was to investigate the possible link between CB1 receptor expression and the presence of metastasis in patients with CRC, investigating the main signaling pathways elicited downstream of CB1 receptor in colon cancer. Fifty-nine consecutive patients, with histologically proven colorectal cancer, were enrolled in the study, of which 30 patients with synchronous metastasis, at first diagnosis and 29 without metastasis. A low expression of CB1 receptor were detected in primary tumor tissue of CRC patients with metastasis and consequently, we observed an alteration of CB1 receptor downstream signaling. These signaling routes were also altered in intestinal normal mucosa, suggesting that, normal mucosa surrounding the tumor provides a realistic picture of the molecules involved in tissue malignant transformation. These observations contribute to the idea that drugs able to induce CB1 receptor expression can be helpful in order to set new anticancer therapeutic strategies. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.
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- 2019
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19. Elevated AA/EPA Ratio Represents an Inflammatory Biomarker in Tumor Tissue of Metastatic Colorectal Cancer Patients
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Maria Lucrezia Benedetto, Valeria Tutino, Maria Gabriella Caruso, Marta Di Masi, Nicola Veronese, Dionigi Lorusso, Valentina De Nunzio, Maria Notarnicola, Tutino, V., De Nunzio, V., Caruso, M.G., Veronese, N., Lorusso, D., Di Masi, M., Benedetto, M.L., and Notarnicola, M.
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Male ,eicosapentaenoic acid ,Colorectal cancer ,medicine.disease_cause ,Metastasis ,lcsh:Chemistry ,Receptor, Cannabinoid, CB2 ,chemistry.chemical_compound ,Medicine ,Arachidonate 15-Lipoxygenase ,Neoplasm Metastasis ,lcsh:QH301-705.5 ,Spectroscopy ,chemistry.chemical_classification ,General Medicine ,Eicosapentaenoic acid ,Computer Science Applications ,Gene Expression Regulation, Neoplastic ,Arachidonic acid ,Female ,lipids (amino acids, peptides, and proteins) ,medicine.symptom ,Colorectal Neoplasms ,Polyunsaturated fatty acid ,Inflammation ,colorectal cancer ,Catalysis ,Article ,Inorganic Chemistry ,Biomarkers, Tumor ,Humans ,metastasis ,RNA, Messenger ,Physical and Theoretical Chemistry ,Molecular Biology ,Aged ,business.industry ,Organic Chemistry ,inflammatory biomarker ,Cancer ,medicine.disease ,PPAR gamma ,lcsh:Biology (General) ,lcsh:QD1-999 ,chemistry ,Cancer research ,business ,Carcinogenesis - Abstract
Chronic inflammation increases the risk of developing certain types of cancer, such as colorectal cancer (CRC). The oxidative metabolism of polyunsaturated fatty acids (PUFAs) has a strong effect on colonic tumorigenesis and the levels of arachidonic acid (AA) and eicosapentaenoic acid (EPA) can contribute to the development of an inflammatory microenvironment. Aim of this study was to evaluate the possible differences in the AA/EPA ratio tissue levels between CRC patients with and without synchronous metastases. Moreover, the expression of the most important inflammatory enzymes and mediators, linked with the AA/EPA ratio, have been also assessed. Sixty-eight patients with CRC were enrolled in the study, of which 33 patients with synchronous metastasis. Fatty acid profile analysis in tissue samples was done to examine the levels of AA and EPA. High levels of the AA/EPA ratio were detected in tumor tissue of patients with metastatic CRC. Moreover, an increase of expression of the main enzymes and mediators involved in inflammation was also detected in the same samples. The lipidomic approach of inflammation allows to evaluate lipid homeostasis changes that occur in cancer and in its metastatic process, in order to identify new biomarkers to be introduced into clinical practice. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.
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- 2019
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20. Women in LOVe: Lacto-Ovo-Vegetarian Diet Rich in Omega-3 Improves Vasomotor Symptoms in Postmenopausal Women. An Exploratory Randomized Controlled Trial
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Palma Aurelia Iacovazzi, Raffaella Licinio, Nicola Veronese, Rossella Donghia, Sara Giannico, Tiziana Daddabbo, Giampiero De Leonardis, Vito Guerra, Rosa Inguaggiato, Rosa Reddavide, Maria Notarnicola, Iris Zinzi, Anna Maria Cisternino, Nicola Giampaolo, Gioacchino Leandro, Valentina De Nunzio, Valeria Tutino, Fabio Fucilli, Ornella Rotolo, Anna Mastrosimini, Maria Gabriella Caruso, Caterina Mammone Rinaldi, Rotolo, O., Zinzi, I., Veronese, N., Cisternino, A.M., Reddavide, R., Inguaggiato, R., Leandro, G., Notarnicola, M., Tutino, V., De Nunzio, V., De Leonardis, G., Guerra, V., Donghia, R., Fucilli, F., Licinio, R., Mastrosimini, A., Rinaldi, C.C.M., Daddabbo, T., Giampaolo, N., Iacovazzi, P.A., Giannico, S., and Caruso, M.G.
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0301 basic medicine ,medicine.medical_specialty ,Time Factors ,Endocrinology, Diabetes and Metabolism ,Sweating ,law.invention ,03 medical and health sciences ,0302 clinical medicine ,Primary outcome ,Randomized controlled trial ,law ,Internal medicine ,Fatty Acids, Omega-6 ,medicine ,Immunology and Allergy ,Humans ,030212 general & internal medicine ,030109 nutrition & dietetics ,Postmenopausal women ,Vasomotor ,business.industry ,Diet, Vegetarian ,Anthropometry ,Middle Aged ,medicine.disease ,Menopause ,Postmenopause ,Vasomotor System ,Treatment Outcome ,Italy ,Hot Flashes ,Observational study ,Female ,Diet, menopause, omega 3, lacto-ovo-vegetarian, RCT, Kupperman index ,business ,Nutritive Value ,Biomarkers ,Olive oil ,Vegetarians - Abstract
Objective:In the postmenopausal period, most women suffer vasomotor symptoms (VMS). It is well-known that VMS can worsen the quality of life. Diet seems to play a relevant role in the development of VMS, but the effect of diet on VMS is mainly limited to observational studies, and analyses of nutritional supplements. The aim of this study was thus to determine the efficacy of a lactoovo- vegetarian (LOVe) diet rich in omega-3 fatty acids vs. a lacto-ovo-vegetarian diet rich in EVO (extra-virgin olive oil) in reducing VMS frequency in postmenopausal women.Methods:A two-arms (lacto-ovo-vegetarian diet with EVO vs. lacto-ovo-vegetarian diet rich in omega-3) randomized-controlled trial with a follow-up period of 16 weeks. We considered as primary outcome the change in the Kupperman index (follow-up vs. baseline evaluation, reported as delta, D) and in its subscales. Secondary outcomes included changes in common anthropometric and biohumoral measurements.Results:Among 54 women randomly assigned to a study group, 40 (mean age 55.1±5.4 years) completed the study and complied with their assigned diet. Women randomized to the omega-3 group (n=18) showed significant improvements, compared to the EVO group (n=22), in Kupperman index (Δ=-11.4±9.8 vs. -5.9±8.2; p=0.045), hot flashes (Δ=-3.3±3.4 vs. -1.3±2.6; p=0.04), and a marginally significant improvement in nervousness (Δ=-1.7±1.7 vs. -0.8±1.5; p=0.07). No significant differences were observed for the secondary outcomes. No relevant side effects were reported.Conclusion:After 16 weeks, a lacto-ovo-vegetarian diet rich in omega-3 reduced VMS frequency in postmenopausal women more than the lacto-ovo-vegetarian diet rich in EVO.
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- 2019
21. Differential Tissue Fatty Acids Profiling between Colorectal Cancer Patients with and without Synchronous Metastasis
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Valeria Tutino, Nicola Veronese, Gianluigi Giannelli, Valentina De Nunzio, Giampiero De Leonardis, Maria Gabriella Caruso, Dionigi Lorusso, Maria Notarnicola, Gisella Marangelli, Vito Guerra, Notarnicola, M., Lorusso, D., Tutino, V., De Nunzio, V., De Leonardis, G., Marangelli, G., Guerra, V., Veronese, N., Caruso, M.G., and Giannelli, G.
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0301 basic medicine ,Colorectal cancer ,Cell ,Catalysis ,Article ,Metastasis ,Inorganic Chemistry ,lcsh:Chemistry ,03 medical and health sciences ,0302 clinical medicine ,lipidomic analysis ,Fatty Acids, Omega-6 ,Fatty Acids, Omega-3 ,medicine ,colon cancer ,metastasis ,fatty acids ,Humans ,Physical and Theoretical Chemistry ,Neoplasm Metastasis ,gamma-Linolenic Acid ,Molecular Biology ,lcsh:QH301-705.5 ,Spectroscopy ,chemistry.chemical_classification ,business.industry ,Organic Chemistry ,Fatty Acids ,Fatty acid ,General Medicine ,medicine.disease ,Eicosapentaenoic acid ,Computer Science Applications ,Red blood cell ,030104 developmental biology ,medicine.anatomical_structure ,chemistry ,lcsh:Biology (General) ,lcsh:QD1-999 ,Eicosapentaenoic Acid ,030220 oncology & carcinogenesis ,Synchronous metastasis ,Cancer research ,metastasi ,lipidomic analysi ,business ,Colorectal Neoplasms ,Polyunsaturated fatty acid - Abstract
The early detection of colorectal cancer and determination of its metastatic potential are important factors to set up more efficacious therapeutic strategies. In the present study, we hypothesize that fatty acids analysis in colorectal cancer patients can discriminate between metastatic and non-metastatic patients. Fifty-one consecutive patients with histologically proven colorectal cancer were enrolled in the study and the presence of synchronous metastasis was detected in 25 of these 51 patients. Fatty acid profile analysis in red blood cell membranes was not able to discriminate the metastatic colorectal cancer patients from those without metastasis. However, significant differences in the tumor tissue fatty acid profile were found in metastatic cancer patients when compared to patients without metastasis. Metastatic patients showed significantly lower percentages of Eicosapentaenoic acid (EPA) and higher levels of γ-linolenic acid (GLA), a n-3- and n-6-Polyunsaturated fatty acid (PUFA), respectively. Our findings, suggesting that membrane lipid rearrangement could influence the cellular function and make the cell more prone to metastasis, offer the opportunity to develop nutritional strategies that may be helpful in the prevention and treatment of colorectal cancer. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.
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- 2018
22. Fatty liver and mortality: a cohort population study in South Italy
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Marisa Chiloiro, Anna Maria Cisternino, Antonio Logroscino, Valeria Tutino, Alberto Rubén Osella, Giovanni Misciagna, Vito Guerra, Rocco Guerra, Ornella Rotolo, Rosa Inguaggiato, Maria Notarnicola, Nicola Veronese, Rossella Donghia, Gioacchino Leandro, Giampiero De Leonardis, Caterina Bonfiglio, Rosa Reddavide, Maria Gabriella Caruso, Caruso, M.G., Veronese, N., Notarnicola, M., Cisternino, A.M., Reddavide, R., Inguaggiato, R., Guerra, V., Donghia, R., Logroscino, A., Rotolo, O., Chiloiro, M., Leandro, G., De Leonardis, G., Tutino, V., Misciagna, G., Bonfiglio, C., Guerra, R., and Osella, A.
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Adult ,Male ,medicine.medical_specialty ,Epidemiology ,Population ,hepatobiliary disease ,03 medical and health sciences ,0302 clinical medicine ,Risk Factors ,Cause of Death ,Internal medicine ,Humans ,Medicine ,Prospective Studies ,Risk factor ,education ,general endocrinology ,Aged ,Aged, 80 and over ,education.field_of_study ,business.industry ,Research ,Fatty liver ,Hepatobiliary disease ,General Medicine ,Middle Aged ,medicine.disease ,mortality ,Fatty Liver ,Survival Rate ,Italy ,Population Surveillance ,030220 oncology & carcinogenesis ,Cohort ,Population study ,Female ,030211 gastroenterology & hepatology ,Alcoholic fatty liver ,Steatosis ,business - Abstract
ObjectiveAlcoholic fatty liver (AFLD) and non-alcoholic fatty liver (NAFLD) are two common conditions. However, if they can increase the risk of death is poorly explored. We therefore aimed to investigate the potential association between the presence and severity of liver steatosis and mortality in a large sample of older people.DesignProspective.SettingCommunity.ParticipantsWomen and men randomly sampled from the electoral rolls of the population of Castellana Grotte, a town in Southern Italy (Apulia region) between 2005 and 2006. Among 1942 initially contacted, 1708 (=87.9%) participated to the baseline survey (Multicentrica Colelitiasi III (MICOL III)). This specific study included 1445 older participants (mean age=65.2 years, females=44.2%).ExposureNAFLD or AFLD.Primary and secondary outcomesMortality (all-cause and specific-cause).ResultsAfter a median of 12 years, 312 participants (=21.6%) died. After adjusting for nine potential confounders, the presence of steatosis was not associated with any increased risk of death in both NAFLD and AFLD. The severity of liver steatosis was not associated with any increased risk of mortality in NAFLD, while in AFLD, the presence of moderate steatosis significantly increased the risk of overall (HR=2.16; 95% CI 1.19 to 3.91) and cancer-specific (HR=3.54; 95% CI 1.16 to 10.87) death.ConclusionsLiver steatosis is not associated with any increased risk of death in NAFLD, while moderate steatosis could be a risk factor for mortality (particularly due to cancer) in people affected by AFLD.
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- 2019
23. Effects of olive oil polyphenols on fatty acid synthase gene expression and activity in human colorectal cancer cells
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Maria Notarnicola, Maria Gabriella Caruso, Maurizio Bifulco, Vincenzo Memeo, Simona Pisanti, Enzo Perri, Valeria Tutino, Maria Teresa Rotelli, Domenica Bocale, Antonio Gentile, Notarnicola, M, Pisanti, S, Tutino, V, Bocale, D, Rotelli, Mt, Gentile, A, Memeo, V, Bifulco, Maurizio, Perri, E, and Caruso, M. G.
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Cell type ,biology ,Cell growth ,Endocrinology, Diabetes and Metabolism ,Cell cycle ,Fas receptor ,Fatty acid synthase ,chemistry.chemical_compound ,chemistry ,Biochemistry ,Apoptosis ,Cell culture ,Genetics ,biology.protein ,Hydroxytyrosol ,Research Paper - Abstract
Oleuropein (OL) and hydroxytyrosol (HT), the main olive oil polyphenols, possess anti-proliferative effects in vitro. Fatty acid synthase, a key anabolic enzyme of biosynthesis of fatty acids, plays an important role in colon carcinoma development. Our aim was to investigate whether gene expression of FAS, as well as its enzymatic activity, is regulated by HT and OL in two human colon cancer cell lines, as HT-29 and SW620. In addition, we investigated the effects of these polyphenols on growth and apoptosis in these cells. FAS gene expression and activity in treated HT-29 and SW620 cells were evaluated by real-time PCR and radiochemical assay, respectively. Cell growth and apoptosis, after polyphenols treatment, were measured by MTT test and flow cytometry, respectively. The inhibition of proliferation, detected after HT treatment, was mediated by an inhibition of FAS expression and its enzymatic activity in SW620 cells, while the anti-proliferative effect in HT-29 cells seems to be independent from FAS. OL exerted an anti-proliferative effect only on SW620 cells with a mechanism which excluded FAS. Olive oil polyphenols used were able to induce apoptosis in both cell lines studied. The increase of apoptosis in these cells was accompanied by the block of cell cycle in the S phase. This study demonstrates that HT and OL may induce anti-proliferative and pro-apoptotic effects only in certain human colorectal cancer cell types. These effects are FAS mediated only in SW620 cells after treatment with HT.
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- 2010
24. Comparative Efficacy of Flow Diverter Devices in the Treatment of Carotid Sidewall Intracranial Aneurysms: a Retrospective, Multicenter Study.
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Dmytriw AA, Salim HA, Musmar B, Cancelliere NM, Griessenauer CJ, Regenhardt RW, Jones J, Tutino V, Hasan Z, Limbucci N, Lay SV, Spears J, Rabinov JD, Harrigan MR, Siddiqui AH, Levy EI, Stapleton CJ, Renieri L, Cognard C, Shaikh H, Kühn AL, Möhlenbruch MA, Tjoumakaris SI, Jabbour P, Taussky P, Settecase F, Heran MKS, Nguyen A, Volders D, Harker P, Devia DA, Puri AS, Psychogios M, Puentes JC, Leone G, Buono G, Tarantino M, Muto M, Briganti F, Dalal S, Gontu V, Alcedo Guardia RE, Vicenty-Padilla JC, Brouwer P, Schmidt MH, Schirmer C, Pickett GE, Andersson T, Söderman M, Marotta TR, Cuellar-Saenz H, Thomas AJ, Patel AB, Mendes Pereira V, and Adeeb N
- Abstract
Background: The comparative efficacy and safety of first-generation flow diverters (FDs), Pipeline Embolization Device (PED) (Medtronic, Irvine, California), Silk (Balt Extrusion, Montmorency, France), Flow Re-direction Endoluminal Device (FRED) (Microvention, Tustin, California), and Surpass Streamline (Stryker Neurovascular, Fremont, California), is not directly established and largely inferred., Purpose: This study aimed to compare the efficacy of different FDs in treating sidewall ICA intracranial aneurysms., Methods: We conducted a retrospective review of prospectively maintained databases from eighteen academic institutions from 2009-2016, comprising 444 patients treated with one of four devices for sidewall ICA aneurysms. Data on demographics, aneurysm characteristics, treatment outcomes, and complications were analyzed. Angiographic and clinical outcomes were assessed using various imaging modalities and modified Rankin Scale (mRS). Propensity score weighting was employed to balance confounding variables. The data analysis used Kaplan-Meier curves, logistic regression, and Cox proportional-hazards regression., Results: While there were no significant differences in retreatment rates, functional outcomes (mRS 0-1), and thromboembolic complications between the four devices, the probability of achieving adequate occlusion at the last follow-up was highest in Surpass device (HR: 4.59; CI: 2.75-7.66, p < 0.001), followed by FRED (HR: 2.23; CI: 1.44-3.46, p < 0.001), PED (HR: 1.72; CI: 1.10-2.70, p = 0.018), and Silk (HR: 1.0 ref. standard). The only hemorrhagic complications were with Surpass (1%)., Conclusion: All the first-generation devices achieved good clinical outcomes and retreatment rates in treating ICA sidewall aneurysms. Prospective studies are needed to explore the nuanced differences between these devices in the long term., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany.)
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- 2024
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25. Filamin A in triple negative breast cancer.
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Giovannelli P, Di Donato M, Licitra F, Sabbatino E, Tutino V, Castoria G, and Migliaccio A
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- Humans, Filamins genetics, Triple Negative Breast Neoplasms drug therapy, Triple Negative Breast Neoplasms pathology
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Triple-negative breast cancer is a rare but highly heterogeneous breast cancer subtype with a limited choice of specific treatments. Chemotherapy remains the only efficient treatment, but its side effects and the development of resistance consolidate the urgent need to discover new targets. In TNBC, filamin A expression correlates to grade and TNM stage. Accordingly, this protein could constitute a new target for this BC subtype. Even if most of the data indicates its direct involvement in cancer progression, some contrasting results underline the need to deepen the studies. To elucidate a possible function of this protein as a TNBC marker, we summarized the main characteristic of filamin A and its involvement in physiological and pathological processes such as cancer. Lastly, we scrutinized its actions in triple-negative breast cancer and highlighted the need to increase the number of studies useful to better clarify the role of this versatile protein as a marker and target in TNBC, alone or in "collaboration" with other proteins with a relevant role in this BC subgroup., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.)
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- 2024
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26. Significant Increase in Oxidative Stress Indices in Erythrocyte Membranes of Obese Patients with Metabolically-Associated Fatty Liver Disease.
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Tutino V, De Nunzio V, Donghia R, Aloisio Caruso E, Cisternino AM, Iacovazzi PA, Mastrosimini AM, Fernandez EA, Giannuzzi V, and Notarnicola M
- Abstract
Metabolic dysfunction-associated hepatic steatosis (MAFLD) indicates the metabolic risk associated with hepatic steatosis, overweight and obesity, and clinical evidence of metabolic dysregulation. Since MAFLD is one of the diseases that show a high frequency of alterations in the lipid content of cell membranes, the aim of this study was to evaluate the indices of oxidative damage of erythrocyte membranes in overweight and obese MAFLD subjects. The study was conducted on serum samples and red blood cell membranes of overweight and obese MAFLD subjects. For each patient, biochemical measurements and lipidomic analyses of erythrocytes membranes were performed. Significant differences in fatty acid profiles of RBC membranes were found between overweight and obese patients. In particular, the Peroxidation Index (PI) was higher in the erythrocyte membranes of obese subjects than in overweight subjects. The same behavior was observed for Unsaturation Index (UI) and Free Radical Stress Index (Free RSI), supporting the fact that the systemic increase in oxidative stress was associated with obesity. The study shows that there is a different susceptibility to erythrocyte membrane peroxidation for overweight and obese subjects, and the increased values of oxidative stress indices observed in the erythrocyte membranes of obese patients with MAFLD may be a possible indicator of pro-oxidative events occurring in obesity-related diseases.
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- 2024
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27. Corrigendum: Managing symptom profile of IBS-D patients with Tritordeum-based foods: results from a pilot study.
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Russo F, Riezzo G, Linsalata M, Orlando A, Tutino V, Prospero L, D'Attoma B, and Giannelli G
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[This corrects the article DOI: 10.3389/fnut.2022.797192.]., (Copyright © 2023 Russo, Riezzo, Linsalata, Orlando, Tutino, Prospero, D'Attoma and Giannelli.)
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- 2023
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28. Corrigendum: Correlation between antioxidant and anticancer activity and phenolic profile of new Apulian table grape genotypes ( V. Vinifera L.).
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Milella RA, De Rosso M, Gasparro M, Gigante I, Debiase G, Forleo LR, Marsico AD, Perniola R, Tutino V, Notarnicola M, Velasco R, and Flamini R
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[This corrects the article DOI: 10.3389/fpls.2022.1064023.]., (Copyright © 2023 Milella, De Rosso, Gasparro, Gigante, Debiase, Forleo, Marsico, Perniola, Tutino, Notarnicola, Velasco and Flamini.)
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- 2023
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29. Plasma-Derived Exosomes from NAFLD Patients Modulate the Cannabinoid Receptors' Expression in Cultured HepaRG Cells.
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De Nunzio V, Carrieri L, Scavo MP, Lippolis T, Cofano M, Caponio GR, Tutino V, Rizzi F, Depalo N, Osella AR, and Notarnicola M
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- Humans, Receptors, Cannabinoid, Receptor, Cannabinoid, CB1 genetics, Non-alcoholic Fatty Liver Disease, Exosomes, Cannabinoids
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Exosomes produced by hepatocytes upon lipotoxic insult play a relevant role in pathogenesis of nonalcoholic fatty liver disease (NAFLD), suggesting an inflammatory response by the activation of monocytes and macrophages and accelerating the disease progression. In the pathogenesis of NAFLD and liver fibrosis, the endogenous cannabinoids and their major receptors CB1 and CB2 appear to be highly involved. This study aimed at evaluating the expression of cannabinoids receptors (CB1R and CB2R) in plasma-derived exosomes extracted from patients with NAFLD, as well as investigating the in vitro effects of the circulating exosomes in cultured human HepaRG cells following their introduction into the culture medium. The results demonstrated that plasma-derived exosomes from NAFLD patients are vehicles for the transport of CB1R and are able to modulate CB receptors' expression in HepaRG cells. In particular, circulating exosomes from NAFLD patients are inflammatory drivers for HepaRG cells, acting through CB1R activation and the downregulation of CB2R. Moreover, CB1R upregulation was associated with increased expression levels of PPAR-γ, a well-known mediator of liver tissue injury. In conclusion, this study provides evidence for CB1R transport by exosomes and suggests that the in vitro effects of circulating exosomes from NAFLD patients are mediated by the expression of cannabinoid receptors.
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- 2023
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30. Correlation between antioxidant and anticancer activity and phenolic profile of new Apulian table grape genotypes ( V. Vinifera L.).
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Milella RA, De Rosso M, Gasparro M, Gigante I, Debiase G, Forleo LR, Marsico AD, Perniola R, Tutino V, Notarnicola M, Velasco R, and Flamini R
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Grapes represent a significant source of phenolic compounds known for their health-promoting properties, such as antioxidant capacity on normal cells and prooxidant activity on tumor cells. The genotype highly affects the polyphenolic composition in grapes and, consequently, the nutritional quality of berries. This work aimed to characterize the phenolic composition, the antioxidant, and anticancer activity of grape skin extracts (GSEs) of nine new table grape genotypes selected from a breeding program to obtain new cultivars of seedless table grapes, well adapted to the climatic change and with higher nutraceutical properties. The grape polyphenolic profile was characterized by Ultra-High-Performance Liquid Chromatography/Quadrupole-Time of Flight mass spectrometry analysis. GSE antioxidant activity was determined by the ABTS, DPPH, and ORAC assays; GSE cell growth inhibition test was carried out in the Caco2 human cancer cell line. The nine GSEs showed different flavonoid and non-flavonoid profiles, and all possessed antioxidant activity, with the 'Aika N.', 'Turese N.', and 'Egnatia N.' the most active. As anticancer activity against the tested cancer cell line, 'Daunia N.' and 'Apenestae N.' showed the EC50 after 24 h of 35.60 µg/mL and 150.91 µg/mL, respectively. The relationship between polyphenolic profile and the antioxidant and anticancer activity of GSE was also investigated. Interestingly, among the different classes of polyphenolics, flavan-3-ols e proanthocyanidins showed the highest positive correlation with the anticancer activity of extracts. These findings can be helpful for the preparation of new extracts for the pharmaceutical and nutraceutical industry and geneticists working in vine breeding programs., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Milella, De Rosso, Gasparro, Gigante, Debiase, Forleo, Marsico, Perniola, Tutino, Notarnicola, Velasco and Flamini.)
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- 2023
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31. Evaluation of the Effects of the Tritordeum-Based Diet Compared to the Low-FODMAPs Diet on the Fecal Metabolome of IBS-D Patients: A Preliminary Investigation.
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Caponio GR, Celano G, Calabrese FM, Riezzo G, Orlando A, D'Attoma B, Ignazzi A, Vacca M, Porrelli A, Tutino V, De Angelis M, Giannelli G, and Russo F
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- Humans, Disaccharides, Pilot Projects, Oligosaccharides, Diet, Metabolome, Fermentation, Diet, Carbohydrate-Restricted, Monosaccharides, Irritable Bowel Syndrome
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Since irritable bowel syndrome (IBS)-a common gastrointestinal (GI) disorder-still lacks effective therapy, a nutritional approach may represent a practical alternative. Different reports demonstrated that a low-fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs) diet (LFD) reduces symptoms in IBS with diarrhea (IBS-D) patients, also inducing beneficial pathophysiological and biochemical modifications. More recently, diets with alternative cereals having a different gluten composition, such as tritordeum, have also been considered (TBD). We investigated the impact of TBD and LFD on the fecal metabolome composition in 38 IBS-D patients randomly allocated to the two diets for 12 weeks. Summarily, at baseline, the profile of fecal volatile organic compounds (VOCs) of IBS-D patients was not significantly different in the two groups. After treatment, significant changes were observed in the two groups regarding the VOCs content since some of them increased in the TBD group (namely, decanoic acid), whereas others (i.e., nonanal and ethanol) increased in the LFD one. Further, at baseline, short-chain fatty acids were positively related to inflammation and showed a significant decreasing trend after both diets compared to baseline values (namely, acetic and propanoic acid). Preliminary results from this pilot study suggest a potential positive intervention of TBD and LFD affecting the fecal metabolome composition in IBS-D patients.
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- 2022
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32. International controlled study of revascularization and outcomes following COVID-positive mechanical thrombectomy.
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Dmytriw AA, Ghozy S, Sweid A, Piotin M, Bekelis K, Sourour N, Raz E, Vela-Duarte D, Linfante I, Dabus G, Kole M, Martínez-Galdámez M, Nimjee SM, Lopes DK, Hassan AE, Kan P, Ghorbani M, Levitt MR, Escalard S, Missios S, Shapiro M, Clarençon F, Elhorany M, Tahir RA, Youssef PP, Pandey AS, Starke RM, El Naamani K, Abbas R, Mansour OY, Galvan J, Billingsley JT, Mortazavi A, Walker M, Dibas M, Settecase F, Heran MKS, Kuhn AL, Puri AS, Menon BK, Sivakumar S, Mowla A, D'Amato S, Zha AM, Cooke D, Vranic JE, Regenhardt RW, Rabinov JD, Stapleton CJ, Goyal M, Wu H, Cohen J, Turkel-Parella D, Xavier A, Waqas M, Tutino V, Siddiqui A, Gupta G, Nanda A, Khandelwal P, Tiu C, Portela PC, Perez de la Ossa N, Urra X, de Lera M, Arenillas JF, Ribo M, Requena M, Piano M, Pero G, De Sousa K, Al-Mufti F, Hashim Z, Nayak S, Renieri L, Du R, Aziz-Sultan MA, Liebeskind D, Nogueira RG, Abdalkader M, Nguyen TN, Vigilante N, Siegler JE, Grossberg JA, Saad H, Gooch MR, Herial NA, Rosenwasser RH, Tjoumakaris S, Patel AB, Tiwari A, and Jabbour P
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- Cross-Sectional Studies, Humans, Male, Retrospective Studies, Thrombectomy methods, Treatment Outcome, Brain Ischemia, COVID-19 complications, Endovascular Procedures methods, Stroke surgery
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Background and Purpose: Previous studies suggest that mechanisms and outcomes in patients with COVID-19-associated stroke differ from those in patients with non-COVID-19-associated strokes, but there is limited comparative evidence focusing on these populations. The aim of this study, therefore, was to determine if a significant association exists between COVID-19 status with revascularization and functional outcomes following thrombectomy for large vessel occlusion (LVO), after adjustment for potential confounding factors., Methods: A cross-sectional, international multicenter retrospective study was conducted in consecutively admitted COVID-19 patients with concomitant acute LVO, compared to a control group without COVID-19. Data collected included age, gender, comorbidities, clinical characteristics, details of the involved vessels, procedural technique, and various outcomes. A multivariable-adjusted analysis was conducted., Results: In this cohort of 697 patients with acute LVO, 302 had COVID-19 while 395 patients did not. There was a significant difference (p < 0.001) in the mean age (in years) and gender of patients, with younger patients and more males in the COVID-19 group. In terms of favorable revascularization (modified Thrombolysis in Cerebral Infarction [mTICI] grade 3), COVID-19 was associated with lower odds of complete revascularization (odds ratio 0.33, 95% confidence interval [CI] 0.23-0.48; p < 0.001), which persisted on multivariable modeling with adjustment for other predictors (adjusted odds ratio 0.30, 95% CI 0.12-0.77; p = 0.012). Moreover, endovascular complications, in-hospital mortality, and length of hospital stay were significantly higher among COVID-19 patients (p < 0.001)., Conclusion: COVID-19 was an independent predictor of incomplete revascularization and poor functional outcome in patients with stroke due to LVO. Furthermore, COVID-19 patients with LVO were more often younger and had higher morbidity/mortality rates., (© 2022 European Academy of Neurology.)
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- 2022
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33. Learning Curve for Flow Diversion of Posterior Circulation Aneurysms: A Long-Term International Multicenter Cohort Study.
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Adeeb N, Dibas M, Griessenauer CJ, Cuellar HH, Salem MM, Xiang S, Enriquez-Marulanda A, Hong T, Zhang H, Taussky P, Grandhi R, Waqas M, Aldine AS, Tutino VM, Aslan A, Siddiqui AH, Levy EI, Ogilvy CS, Thomas AJ, Ulfert C, Möhlenbruch MA, Renieri L, Bengzon Diestro JD, Lanzino G, Brinjikji W, Spears J, Vranic JE, Regenhardt RW, Rabinov JD, Harker P, Müller-Thies-Broussalis E, Killer-Oberpfalzer M, Islak C, Kocer N, Sonnberger M, Engelhorn T, Kapadia A, Yang VXD, Salehani A, Harrigan MR, Krings T, Matouk CC, Mirshahi S, Chen KS, Aziz-Sultan MA, Ghorbani M, Schirmer CM, Goren O, Dalal SS, Finkenzeller T, Holtmannspötter M, Buhk JH, Foreman PM, Cress MC, Hirschl RA, Reith W, Simgen A, Janssen H, Marotta TR, Stapleton CJ, Patel AB, and Dmytriw AA
- Subjects
- Humans, Learning Curve, Treatment Outcome, Cohort Studies, Retrospective Studies, Stents, Endovascular Procedures methods, Intracranial Aneurysm diagnostic imaging, Intracranial Aneurysm surgery, Embolization, Therapeutic methods
- Abstract
Background and Purpose: Flow diversion has gradually become a standard treatment for intracranial aneurysms of the anterior circulation. Recently, the off-label use of the flow diverters to treat posterior circulation aneurysms has also increased despite initial concerns of rupture and the suboptimal results. This study aimed to explore the change in complication rates and treatment outcomes across time for posterior circulation aneurysms treated using flow diversion and to further evaluate the mechanisms and variables that could potentially explain the change and outcomes., Materials and Methods: A retrospective review using a standardized data set at multiple international academic institutions was performed to identify patients with ruptured and unruptured posterior circulation aneurysms treated with flow diversion during a decade spanning January 2011 to January 2020. This period was then categorized into 4 intervals., Results: A total of 378 procedures were performed during the study period. Across time, there was an increasing tendency to treat more vertebral artery and fewer large vertebrobasilar aneurysms ( P = .05). Moreover, interventionalists have been increasingly using fewer overlapping flow diverters per aneurysm ( P = .07). There was a trend toward a decrease in the rate of thromboembolic complications from 15.8% in 2011-13 to 8.9% in 2018-19 ( P = .34)., Conclusions: This multicenter experience revealed a trend toward treating fewer basilar aneurysms, smaller aneurysms, and increased usage of a single flow diverter, leading to a decrease in the rate of thromboembolic and hemorrhagic complications., (© 2022 by American Journal of Neuroradiology.)
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- 2022
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34. Beneficial Effects of Table Grape Use on Serum Levels of Omega-3 Index and Liver Function: A Randomized Controlled Clinical Trial.
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Notarnicola M, De Nunzio V, Lippolis T, Tutino V, Cisternino AM, Iacovazzi PA, Milella RA, Gasparro M, Negro R, Polignano M, and Caruso MG
- Abstract
This clinical trial was aimed to investigate the effects of fresh table grape intake on the serum levels of the Omega-3 index, defined as the sum of eicosapentaenoic acid (EPA) + docosahexaenoic acid (DHA) levels. Forty consecutive healthy subjects were randomly assigned to the control group, receiving only dietary recommendations, and the grape group receiving a daily dose of 5 g of fresh table grape per kg of body weight, for 21 days. Compared with baseline, the grape treatment produced no significant difference in the serum levels of glucose, liver transaminase, and triglycerides, with the exception of cholesterol value, which was significantly reduced in both control and grape group (180.5 ± 20.32 vs. 196.1 ± 30.0 and 181.4 ± 21.9 vs. 194.3 ± 37.5, respectively). After 4 weeks from the end of grape treatment, the analysis of single fatty acids showed a significant increase in oleic acid content (14.15 ± 1.8 vs. 12.85 ± 1.6, p < 0.05) and a significant induction of the Omega-3 index (8.23 ± 1.9 vs. 6.09 ± 1.2, p < 0.05), associated with increased serum levels of adiponectin (24.09 ± 1.08 vs. 8.8 ± 0.7, p < 0.001). In contrast, the expression of fibroblast growth factor 21 (FGF21), a molecule associated with metabolic syndrome and liver disease, was significantly reduced (37.9 ± 6.8 vs. 107.8 ± 10.1, p < 0.001). The data suggest that the intake of fresh grape improves the Omega-3 index in the serum and exerts beneficial effects on liver function through the overexpression of adiponectin and the reduction in FGF21 levels.
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- 2022
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35. Nutraceuticals: Focus on Anti-Inflammatory, Anti-Cancer, Antioxidant Properties in Gastrointestinal Tract.
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Caponio GR, Lippolis T, Tutino V, Gigante I, De Nunzio V, Milella RA, Gasparro M, and Notarnicola M
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In recent years, nutraceuticals have gained great popularity, owing to their physiological and potential health effects, such as anti-inflammatory, anti-cancer, antioxidant, and prebiotic effects, and their regulation of lipid metabolism. Since the Mediterranean diet is a nutritionally recommended dietary pattern including high-level consumption of nutraceuticals, this review aimed to summarize the main results obtained by our in vitro and in vivo studies on the effects of the major constituents of the Mediterranean diet (i.e., extra virgin olive oil compounds, polyunsaturated fatty acids, and fruit components). Based on experimental studies, the therapeutic purpose of nutraceuticals depends on their bioavailability, solubility, toxicity, and delivery system. This review provides more in-depth knowledge on the effects linked to nutraceuticals administration on human health, focusing the gastrointestinal tract and suggesting specific dietary components for personalized adjuvant therapies.
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- 2022
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36. Managing Symptom Profile of IBS-D Patients With Tritordeum-Based Foods: Results From a Pilot Study.
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Russo F, Riezzo G, Linsalata M, Orlando A, Tutino V, Prospero L, D'Attoma B, and Giannelli G
- Abstract
In the past few years, increasing attention has been given to the pathologic role of specific foods in IBS, like wheat and other cereals. Recent literature describes IBS patients who may experience gastrointestinal (GI) and extra-GI symptoms precipitated by the ingestion of cereals. Tritordeum is a cereal of Spanish origin derived from the hybridization of durum wheat and wild barley. It is different from classic wheat for its gluten protein composition, with fewer carbohydrates and fructans and a higher content of proteins, dietary fibers, and antioxidants. This pilot study aimed to investigate the effects of a 12-week diet with Tritordeum-based foods in substitution of other cereals on the profile of GI symptoms (evaluated by appropriate questionnaire) and the health of the GI barrier (assessed by sugar absorption test and different markers of integrity and functions) in 16 diarrhea-predominant IBS (IBS-D) patients. The diet with Tritordeum-based foods (bread, bakery products, and pasta) significantly reduced IBS-D patients' symptoms. This amelioration appears to occur through an overall improvement of the GI barrier, as demonstrated by the reduced intestinal permeability and the decreased levels of markers of intestinal mucosal integrity, mucosal inflammation, and fermentative dysbiosis., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Russo, Riezzo, Linsalata, Orlando, Tutino, Prospero, D'Attoma and Giannelli.)
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- 2022
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37. Initial evaluation of 2D and 3D simulated high-speed 1000 fps vascular contrast-flow image sequences using computational fluid dynamics (CFD).
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Shields A, Williams K, Veeturi SS, Tutino V, Ionita C, Bednarek DR, and Rudin S
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Digital subtraction angiography (DSA) remains the clinical standard for detailed visualization of the neurovasculature due to its high-spatial resolution; however, detailed blood-flow quantification is impaired by its low-temporal resolution. Advances in photon-counting detector technology have led us to develop High-Speed Angiography (HSA), where x-ray images are acquired at 1000 fps for more accurate visualization and quantification of blood flow. We have implemented a physics-based optical flow method to extract such information from HSA, but validation of the angiography-derived velocity distributions is not straightforward. Computational fluid dynamics (CFD) is widely regarded as the benchmark for hemodynamic analysis, as it provides a multitude of quantitative flow parameters throughout the volume of interest. However, there are several limitations with this method related to over-simplification of boundary conditions and suboptimal meshing (spatial resolution), that make CFD simulation results an inexact criterion for validation. To overcome this issue for HSA validation, CFD was used to generate both simulated high-speed angiograms and the corresponding ground-truth 3D flow fields to better understand the relationship between the 3D volumetric-flow distribution and the 2D projected-flow distribution as is obtained with angiography, and the subsequent 2D approximation of flow velocity. Several geometries were investigated, ranging from simple pipe models to complex patient-specific aneurysms. Simulated datasets were analyzed with the optical flow algorithm, and the effects of flow divergence, quantum mottle, and intensity gradient on the calculation were evaluated. From these simulations, we can evaluate whether flow fields reconstructed from HSA are representative of significant flow patterns in the 3D vasculature.
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- 2022
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38. Effects of weight change on all causes, digestive system and other causes mortality in Southern Italy: a competing risk approach.
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Campanella A, Sorino P, Bonfiglio C, Mirizzi A, Franco I, Bianco A, Misciagna G, Caruso MG, Cisternino AM, Notarnicola M, Tutino V, D'Attoma B, and Osella AR
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- Adult, Body Mass Index, Female, Humans, Italy epidemiology, Longitudinal Studies, Male, Middle Aged, Prospective Studies, Body-Weight Trajectory, Digestive System physiopathology, Mortality trends
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Weight change is associated with all causes of death, cardiovascular, and cancer mortality and a heterogeneous group of other causes of death. We aimed to estimate the effect of weight change on all causes and cause-specific mortality in a cohort with a high prevalence of deaths due to diseases of the digestive system.MethodsIn this prospective cohort study, 2230 subjects aged 30 to 50 years were examined. The study consisted of a 32-year longitudinal study period (January 1985 to December 2017) and mortality follow-up. Outcomes were mortality from all causes and deaths from gastrointestinal disease. Root Mean Squared Error (RMSE) was evaluated to capture individual residual variation in Body Mass Index (BMI) after adjustment for baseline BMI, and the relationship of residual variation with mortality was calculated as cumulative incidence function and cause-specific hazard (CSH) rate.ResultsIn total, 793 participants died during the follow-up, 96 of them due to Digestive system causes. Magnitude of residual variation weight in the last quintile was associated with all-cause mortality (relative risk, 2.00; 95% CI, 1.54-2.59) and Digestive system causes (relative risk, 3.82; 95% CI, 1.86-7.81).ConclusionThe findings suggest an association between weight change and gastrointestinal disease mortality. Epidemiological works studying the correlation between weight change and mortality should consider this aspect., (© 2021. The Author(s), under exclusive licence to Springer Nature Limited.)
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- 2022
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39. Impact of Fresh Table Grape Intake on Circulating microRNAs Levels in Healthy Subjects: A Significant Modulation of Gastrointestinal Cancer-Related Pathways.
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Tutino V, De Nunzio V, Milella RA, Gasparro M, Cisternino AM, Gigante I, Lanzilotta E, Iacovazzi PA, Lippolis A, Lippolis T, Caruso MG, and Notarnicola M
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- Healthy Volunteers, Humans, Circulating MicroRNA, Gastrointestinal Neoplasms, MicroRNAs, Vitis
- Abstract
Scope: The study aims to investigate the effects of fresh table grape consumption in healthy subjects on circulating levels of the most common human microRNAs (miRNAs). The regulatory network governed by these modulated miRNAs is also investigated., Methods and Results: Autumn Royal table grape, used in this study, is chosen for its high polyphenolic content and antioxidant properties. The study is a randomized controlled trial, in which 40 consecutive subjects are recruited on a voluntary basis and randomly assigned to two groups of the study, the control group, receiving only dietary recommendations and a grape group receiving a daily dose of 5 g of fresh table grape per kg of body weight for 21 days. All analyses are performed at baseline and after 21 days of dietary treatment. Circulating miRNAs levels are detected by Real-Time quantitative PCR (RT-qPCR) followed by bioinformatic functional analysis. The study identifies 20 circulating miRNAs differentially expressed in healthy subjects after grape intake, and in particular, 18 of 20 are down-regulated and 2 are up-regulated., Conclusion: The dietary intake of table grape affects circulating miRNAs levels in healthy subjects, particularly the miRNAs related to pathways involved in counteracting cancer development, including gastrointestinal cancers., (© 2021 Wiley-VCH GmbH.)
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- 2021
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40. Use of patient specific 3D printed neurovascular phantoms to simulate mechanical thrombectomy.
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Sommer KN, Bhurwani MMS, Tutino V, Siddiqui A, Davies J, Snyder K, Levy E, Mokin M, and Ionita CN
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Background: The ability of the patient specific 3D printed neurovascular phantoms to accurately replicate the anatomy and hemodynamics of the chronic neurovascular diseases has been demonstrated by many studies. Acute occurrences, however, may still require further development and investigation and therefore we studied acute ischemic stroke (AIS). The efficacy of endovascular procedures such as mechanical thrombectomy (MT) for the treatment of large vessel occlusion (LVO), can be improved by testing the performance of thrombectomy devices and techniques using patient specific 3D printed neurovascular models., Methods: 3D printed phantoms were connected to a flow loop with physiologically relevant flow conditions, including input flow rate and fluid temperature. A simulated blood clot was introduced into the model and placed in the proximal Middle Cerebral Artery (MCA) region. Clot location, composition, length, and arterial angulation were varied and MTs were simulated using stent retrievers. Device placement relative to the clot and the outcome of the thrombectomy were recorded for each situation. Digital subtraction angiograms (DSA) were captured before and after LVO simulation. Recanalization outcome was evaluated using DSA as either 'no recanalization' or 'recanalization'. Forty-two 3DP neurovascular phantom benchtop experiments were performed., Results: Clot angulation within the MCA region had the most significant impact on the MT outcome, with a p-value of 0.016. Other factors such as clot location, clot composition, and clot length correlated weakly with the MT outcome., Conclusions: This project allowed us to gain knowledge of how such characteristics influence thrombectomy success and can be used in making clinical decisions when planning the procedure and selecting specific thrombectomy tools and approaches., (© 2021. The Author(s).)
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- 2021
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41. Nonalcoholic Fatty Liver Disease: Focus on New Biomarkers and Lifestyle Interventions.
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Notarnicola M, Osella AR, Caruso MG, Pesole PL, Lippolis A, Tutino V, Bonfiglio C, De Nunzio V, Scavo MP, Mirizzi A, Franco I, Lippolis T, D'Alessandro R, Refolo MG, and Messa C
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- Animals, Cytokines metabolism, Diet, Disease Management, Exercise, Exosomes, Fatty Acids metabolism, Health Behavior, Hepatocytes metabolism, Humans, Life Style, Lipid Metabolism, Lipidomics, Lipids blood, Microbiota, Non-alcoholic Fatty Liver Disease diagnosis, Biomarkers, Disease Susceptibility, Non-alcoholic Fatty Liver Disease etiology, Non-alcoholic Fatty Liver Disease metabolism
- Abstract
Nonalcoholic fatty liver disease (NAFLD) is considered a hepatic manifestation of metabolic syndrome, characterized from pathological changes in lipid and carbohydrate metabolism. Its main characteristics are excessive lipid accumulation and oxidative stress, which create a lipotoxic environment in hepatocytes leading to liver injury. Recently, many studies have focused on the identification of the genetic and epigenetic modifications that also contribute to NAFLD pathogenesis and their prognostic implications. The present review is aimed to discuss on cellular and metabolic alterations associated with NAFLD, which can be helpful to identify new noninvasive biomarkers. The identification of accumulated lipids in the cell membranes, as well as circulating cytokeratins and exosomes, provides new insights in understanding of NAFLD. This review also suggests that lifestyle modifications remain the main prevention and/or treatment for NAFLD.
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- 2021
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42. The Relationship between Low Serum Vitamin D Levels and Altered Intestinal Barrier Function in Patients with IBS Diarrhoea Undergoing a Long-Term Low-FODMAP Diet: Novel Observations from a Clinical Trial.
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Linsalata M, Riezzo G, Orlando A, D'Attoma B, Prospero L, Tutino V, Notarnicola M, and Russo F
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- Adult, Biomarkers blood, Body Mass Index, Diarrhea complications, Feces chemistry, Female, Fermentation, Gastrointestinal Diseases complications, Haptoglobins, Humans, Irritable Bowel Syndrome physiopathology, Male, Middle Aged, Protein Precursors blood, Diarrhea diet therapy, Diet, Carbohydrate-Restricted methods, Gastrointestinal Diseases diet therapy, Irritable Bowel Syndrome diet therapy, Vitamin D blood
- Abstract
Decreased serum vitamin D (VD) levels have been associated with gastrointestinal (GI) disorders, including irritable bowel syndrome (IBS). VD can also modulate the intestinal barrier. Given the link between the GI barrier's alterations and diet, attention has aroused the positive effects of the Low FODMAP Diet (LFD) on IBS patients' symptom profile. We evaluated the GI symptoms and the urinary and circulating markers of GI barrier function, the markers of inflammation and intestinal dysbiosis in 36 IBS patients with predominant diarrhea (IBS-D) (5 men and 31 women, 43.1 ± 1.7 years) categorized for their circulating VD levels in low (L-VD) and normal (N-VD) (cutoff = 20 ng/mL). Evaluations were performed before and after 12 weeks of LFD. At the baseline, L-VD patients showed a significantly worse symptom profile and altered small intestinal permeability (s-IP) than N-VD. After LFD, a significant increase in the circulating VD levels in both the subgroups and a significant improvement of s-IP in L-VD patients occurred. Finally, VD levels negatively correlated with the symptom score and fecal zonulin. These data highlight the close relationship between VD and the intestinal barrier and support their involvement in IBS-D pathophysiology. Moreover, the potentially positive role of LFD in the management of IBS-D was confirmed.
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- 2021
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43. Cannabinoid Receptors Overexpression in a Rat Model of Irritable Bowel Syndrome (IBS) after Treatment with a Ketogenic Diet.
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Gigante I, Tutino V, Russo F, De Nunzio V, Coletta S, Armentano R, Crovace A, Caruso MG, Orlando A, and Notarnicola M
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- Animals, Cannabinoids metabolism, Diet, Ketogenic adverse effects, Disease Models, Animal, Endocannabinoids genetics, Humans, Intestinal Mucosa drug effects, Intestinal Mucosa metabolism, Irritable Bowel Syndrome genetics, Irritable Bowel Syndrome pathology, Rats, Irritable Bowel Syndrome diet therapy, Receptor, Cannabinoid, CB1 genetics, Receptor, Cannabinoid, CB2 genetics, Receptors, Cannabinoid genetics
- Abstract
The administration of a ketogenic diet (KD) has been considered therapeutic in subjects with irritable bowel syndrome (IBS). This study aimed to investigate the molecular mechanisms by which a low-carbohydrate diet, such as KD, can improve gastrointestinal symptoms and functions in an animal model of IBS by evaluating possible changes in intestinal tissue expression of endocannabinoid receptors. In rats fed a KD, we detected a significant restoration of cell damage to the intestinal crypt base, a histological feature of IBS condition, and upregulation of CB1 and CB2 receptors. The diet also affected glucose metabolism and intestinal membrane permeability, with an overexpression of the glucose transporter GLUT1 and tight junction proteins in treated rats. The present data suggest that CB receptors represent one of the molecular pathways through which the KD works and support possible cannabinoid-mediated protection at the intestinal level in the IBS rats after dietary treatment.
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- 2021
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44. Increased Perviousness on CT for Acute Ischemic Stroke is Associated with Fibrin/Platelet-Rich Clots.
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Patel TR, Fricano S, Waqas M, Tso M, Dmytriw AA, Mokin M, Kolega J, Tomaszewski J, Levy EI, Davies JM, Snyder KV, Siddiqui AH, and Tutino VM
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- Aged, Blood Platelets pathology, Computed Tomography Angiography methods, Female, Fibrin analysis, Humans, Ischemic Stroke pathology, Male, Thrombectomy methods, Thrombosis pathology, Ischemic Stroke diagnostic imaging, Ischemic Stroke surgery, Thrombosis diagnostic imaging, Treatment Outcome
- Abstract
Background and Purpose: Clot perviousness in acute ischemic stroke is a potential CT imaging biomarker for mechanical thrombectomy efficacy. We investigated the association among perviousness, clot cellular composition, and first-pass effect., Materials and Methods: In 40 mechanical thrombectomy-treated cases of acute ischemic stroke, we calculated perviousness as the difference in clot density on CT angiography and noncontrast CT. We assessed the proportion of fibrin/platelet aggregates, red blood cells, and white blood cells on clot histopathology. We tested for linear correlation between histologic components and perviousness, differences in components between "high" and "low" pervious clots defined by median perviousness, and differences in perviousness/composition between cases that did and did not achieve a first-pass effect., Results: Perviousness significantly positively and negatively correlated with the percentage of fibrin/platelet aggregates ( P = .001) and the percentage of red blood cells ( P = .001), respectively. Higher pervious clots had significantly greater fibrin/platelet aggregate content ( P = .042). Cases that achieved a first-pass effect ( n = 14) had lower perviousness, though not significantly ( P = .055). The percentage of red blood cells was significantly higher ( P = .028) and the percentage of fibrin/platelet aggregates was significantly lower ( P = .016) in cases with a first-pass effect. There was no association between clot density on NCCT and clot composition or first-pass effect. Receiver operating characteristic analysis indicated that clot composition was the best predictor of first-pass effect (area under receiver operating characteristic curve: percentage of fibrin/platelet aggregates = 0.731, percentage of red blood cells = 0.706, perviousness = 0.668)., Conclusions: Clot perviousness on CT is associated with a higher percentage of fibrin/platelet aggregate content. Histologic data and, to a lesser degree, perviousness may have value in predicting first-pass outcome. Imaging metrics that more strongly reflect clot biology than perviousness may be needed to predict a first-pass effect with high accuracy., (© 2021 by American Journal of Neuroradiology.)
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- 2021
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45. Physical Activity and Low Glycemic Index Mediterranean Diet: Main and Modification Effects on NAFLD Score. Results from a Randomized Clinical Trial.
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Franco I, Bianco A, Mirizzi A, Campanella A, Bonfiglio C, Sorino P, Notarnicola M, Tutino V, Cozzolongo R, Giannuzzi V, Aballay LR, Buongiorno C, Bruno I, and Osella AR
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- Adult, Behavior Therapy, Female, Humans, Life Style, Male, Middle Aged, Diet, Mediterranean, Exercise, Glycemic Index, Non-alcoholic Fatty Liver Disease
- Abstract
Background: Non-Alcoholic Fatty Liver Disease (NAFLD) is the most common chronic liver disease worldwide, and lifestyle modification is the current standard treatment. The aim of the study was to estimate the effect of two different physical activity (PA) programs, a Low Glycemic Index Mediterranean Diet (LGIMD), and their combined effect on the NAFLD score as measured by FibroScan
® ., Methods: Moderate or severe NAFLD subjects ( n = 144) were randomly assigned to six intervention arms during three months. Interventions arms were a control diet, LGIMD, aerobic activity program (PA1), combined activity program (PA2), and LGIMD plus PA1 or LGIMD plus PA2. The data were compared at baseline, at 45 days, and at 90 days. Analysis of variance was performed under the intention-to-treat principle., Results: There was a statistically significant reduction in the NAFLD score after 45 days of treatment in every working arm except for Arm 1 (control diet). After 90 days, the best results were shown by the intervention arms in which LGIMD was associated with PA: LGIMD plus PA1 (-61.56 95% CI -89.61, -33.50) and LGIMD plus PA2 (-38.15 95% CI -64.53, -11.77)., Conclusion: All treatments were effective to reduce NAFLD scores, but LGIMD plus PA1 was the most efficient.- Published
- 2020
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46. Colorectal Cancer and Bone Tissue: Fantastic Relations and Where to Find Them.
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Gigante I, Tutino V, De Nunzio V, and Notarnicola M
- Abstract
Colorectal cancer (CRC) is the third most common cancer worldwide. There is a need for the early diagnosis of CRC for a better prognostic outcome. It is, therefore, crucial to understand the CRC pathogenesis in all its aspects. In many cases, one of the main causes of cancer-related deaths is the presence of metastases. In this context, an often overlooked aspect is the metastatic tropism, since CRC, like other cancers, is more prone to metastasize some organs rather than others. Beyond the liver and lung, and differently from other types of cancers, a not usual site of CRC metastases is the bone. However, it may assume a crucial role in the development and the outcome of the disease. Therefore, this review aims to discuss the complex relations between bone markers and CRC pathogenesis, suggesting the use of these molecules as potential targets for therapeutic purposes. Different osteogenic molecules, some of whom are growth factors and are implicated in the different osteogenic pathways, have been proved to also be involved in CRC progression. Some of them are oncogenes, while others oncosuppressors, and in a future perspective, some of them may represent new potential CRC biomarkers.
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- 2020
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47. Flavonoid and Non-Flavonoid Compounds of Autumn Royal and Egnatia Grape Skin Extracts Affect Membrane PUFA's Profile and Cell Morphology in Human Colon Cancer Cell Lines.
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Tutino V, Gigante I, Milella RA, De Nunzio V, Flamini R, De Rosso M, Scavo MP, Depalo N, Fanizza E, Caruso MG, and Notarnicola M
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- Antineoplastic Agents, Phytogenic chemistry, Arachidonate 15-Lipoxygenase genetics, Caco-2 Cells, Cell Line, Tumor, Cell Membrane drug effects, Colonic Neoplasms drug therapy, Colonic Neoplasms genetics, Flavonoids chemistry, Gas Chromatography-Mass Spectrometry, Gene Expression Regulation, Neoplastic drug effects, Humans, Lipidomics, PPAR gamma genetics, Plant Extracts chemistry, Plant Extracts pharmacology, Vitis classification, Antineoplastic Agents, Phytogenic pharmacology, Cell Membrane chemistry, Colonic Neoplasms metabolism, Fatty Acids, Unsaturated analysis, Flavonoids pharmacology, Vitis chemistry
- Abstract
Grapes contain many flavonoid and non-flavonoid compounds with anticancer effects. In this work we fully characterized the polyphenolic profile of two grape skin extracts (GSEs), Autumn Royal and Egnatia, and assessed their effects on Polyunsaturated Fatty Acid (PUFA) membrane levels of Caco2 and SW480 human colon cancer cell lines. Gene expression of 15-lipoxygenase-1 (15-LOX-1), and peroxisome proliferator-activated receptor gamma (PPAR-γ), as well as cell morphology, were evaluated. The polyphenolic composition was analyzed by Ultra-High-Performance Liquid Chromatography/Quadrupole-Time of Flight mass spectrometry (UHPLC/QTOF) analysis. PUFA levels were evaluated by gas chromatography, and gene expression levels of 15-LOX-1 and PPAR-γ were analyzed by real-time Polymerase Chain Reaction (PCR). Morphological cell changes caused by GSEs were identified by field emission scanning electron microscope (FE-SEM) and photomicrograph examination. We detected a different profile of flavonoid and non-flavonoid compounds in Autumn Royal and Egnatia GSEs. Cultured cells showed an increase of total PUFA levels mainly after treatment with Autumn Royal grape, and were richer in flavonoids when compared with the Egnatia variety. Both GSEs were able to affect 15-LOX-1 and PPAR-γ gene expression and cell morphology. Our results highlighted a new antitumor mechanism of GSEs that involves membrane PUFAs and their downstream pathways.
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- 2020
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48. Improved Symptom Profiles and Minimal Inflammation in IBS-D Patients Undergoing a Long-Term Low-FODMAP Diet: A Lipidomic Perspective.
- Author
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Orlando A, Tutino V, Notarnicola M, Riezzo G, Linsalata M, Clemente C, Prospero L, Martulli M, D'Attoma B, De Nunzio V, and Russo F
- Subjects
- Adolescent, Adult, Aged, Arachidonic Acid metabolism, C-Reactive Protein metabolism, Cyclooxygenase 2 metabolism, Dinoprostone metabolism, Disaccharides, Eicosapentaenoic Acid metabolism, Erythrocyte Membrane metabolism, Fatty Acids metabolism, Female, Fermentation, Humans, Inflammation Mediators metabolism, Male, Middle Aged, Monosaccharides, Oligosaccharides, Polymers, Treatment Outcome, Young Adult, Diet, Carbohydrate-Restricted, Irritable Bowel Syndrome diet therapy, Irritable Bowel Syndrome metabolism
- Abstract
Given the link between the minimal inflammation underlying irritable bowel syndrome (IBS) and dietary treatments, considerable attention has focused on diets low in fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs). In this context, inflammatory patterns and lipidomic investigations may shed light on the pathophysiological mechanisms whereby a low-FODMAP diet (LFD) improves the IBS diarrhoea (IBS-D) variant. Thus, we investigated whether a long-term LFD induced changes in symptom profiles, anthropometric characteristics, inflammatory markers (C-reactive protein, cyclooxygenase-2, and prostaglandin E2) and erythrocyte-membrane fatty acid (FA) composition in IBS-D patients. Twenty IBS-D patients underwent a 90 day personalised LFD programme, and were regularly evaluated at scheduled visits. At the diet's end, both IBS symptoms and anthropometric parameters were significantly improved. A significant decrease in prostaglandin E2 also accompanied these reductions. As for FAs, the putative inflammatory indicators, arachidonic acid (AA) levels and the AA/eicosapentaenoic acid ratio were significantly decreased. In conclusion, IBS-D patients following a controlled long-term LFD experienced improved symptom profiles and decreased inflammatory markers linked to FAs. Lipidomic data may be insightful for unravelling the molecular mechanisms associated with IBS-D pathophysiology., Competing Interests: The authors declare no conflict of interest.
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- 2020
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49. Stearoyl-CoA Desaturase-1 Enzyme Inhibition by Grape Skin Extracts Affects Membrane Fluidity in Human Colon Cancer Cell Lines.
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Tutino V, Gigante I, Scavo MP, Refolo MG, Nunzio V, Milella RA, Caruso MG, and Notarnicola M
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- Cell Line, Tumor, Cell Movement drug effects, Cell Proliferation drug effects, Colonic Neoplasms, Enzyme Inhibitors chemistry, Humans, Plant Extracts chemistry, Polyphenols chemistry, Polyphenols pharmacology, Enzyme Inhibitors pharmacology, Membrane Fluidity drug effects, Plant Extracts pharmacology, Stearoyl-CoA Desaturase antagonists & inhibitors, Vitis chemistry
- Abstract
The polyphenolic compounds present in grape extracts have chemopreventive and anticancer properties. Here, we studied the ability of two grape skin extracts (GSEs), Autumn Royal and Egnatia, to influence the cell motility and membrane fluidity regulated by the enzyme Stearoyl-CoA desaturase-1 (SCD1) which increases with the cancer aggressiveness. Caco2 and SW480 human colon cancer cell lines were treated with increasing concentrations of GSEs to evaluate cell proliferation and motility. SCD1 levels were evaluated in both treated cell lines, by membrane lipidomic analysis conducted by gas chromatography. The expression levels of SCD1 and other factors involved in the reorganization of the cytoskeleton and focal adhesions were assessed by Real-time PCR, Western Blotting, and Immunofluorescence staining. High-performance liquid chromatography (HPLC) analyses were performed to determine the phenolic composition in the GSEs, finding them more expressed in Autumn Royal than in Egnatia. Both treatments reduced the levels of SCD1, phospho-Rac1/Cdc42/Rac1/Cdc42 ratio, Cofilin, Vimentin, and phospho-Paxillin especially in Caco2 compared to SW480, showing a different behavior of the two cell lines to these natural compounds. Our findings show that GSEs block the cell migration and membrane fluidity through a new mechanism of action involving structural cellular components., Competing Interests: The authors declare no conflict of interest.
- Published
- 2020
- Full Text
- View/download PDF
50. Exosomes for Diagnosis and Therapy in Gastrointestinal Cancers.
- Author
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Scavo MP, Depalo N, Tutino V, De Nunzio V, Ingrosso C, Rizzi F, Notarnicola M, Curri ML, and Giannelli G
- Subjects
- Animals, Biomarkers, Tumor genetics, Drug Delivery Systems methods, Exosomes genetics, Frizzled Receptors genetics, Frizzled Receptors metabolism, Gastrointestinal Neoplasms diagnosis, Gastrointestinal Neoplasms drug therapy, Humans, Lipid Metabolism, Biomarkers, Tumor metabolism, Exosomes metabolism, Gastrointestinal Neoplasms metabolism
- Abstract
Exosomes are membrane-bound extracellular vesicles (EVs) released by most cells, having a size ranging from 30 to 150 nm, and are involved in mechanisms of cell-cell communication in physiological and pathological tissues. Exosomes are engaged in the transport of biomolecules, such as lipids, proteins, messenger RNAs , and micro RNA , and in signal transmission through the intercellular transfer of components. In the context of proteins and nucleic acids transported from exosomes, our interest is focused on the Frizzled proteins family and related messenger RNA . Exosomes can regenerate stem cell phenotypes and convert them into cancer stem cells by regulating the Wnt pathway receptor family, namely Frizzled proteins. In particular, for gastrointestinal cancers, the Frizzled protein involved in those mechanisms is Frizzled-10 (FZD-10). Currently, increasing attention is being devoted to the protein and lipid composition of exosomes interior and membranes, representing profound knowledge of specific exosomes composition fundamental for their application as new delivering drug tools for cancer therapy. This review intends to cover the most recent literature on the use of exosome vesicles for early diagnosis, follow-up, and the use of these physiological nanovectors as drug delivery systems for gastrointestinal cancer therapy.
- Published
- 2020
- Full Text
- View/download PDF
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