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1. Serum immunoglobulin G and mucosal immunoglobulin A antibodies from prepandemic samples collected in Kilifi, Kenya, neutralize SARS-CoV-2 in vitro

Author

Nyagwange, James, Kutima, Bernadette, Mwai, Kennedy, Karanja, Henry K., Gitonga, John N., Mugo, Daisy, Sein, Yiakon, Wright, Daniel, Omuoyo, Donwilliams O., Nyiro, Joyce U., Tuju, James, Nokes, D. James, Agweyu, Ambrose, Bejon, Philip, Ochola-Oyier, Lynette I., Scott, J． Anthony G., Lambe, Teresa, Nduati, Eunice, Agoti, Charles, and Warimwe, George M.

Published
2023
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2. Full-length MSP1 is a major target of protective immunity after controlled human malaria infection

Author

Rosenkranz, Micha, primary, Nkumama, Irene N, additional, Ogwang, Rodney, additional, Kraker, Sara, additional, Blickling, Marie, additional, Mwai, Kennedy, additional, Odera, Dennis, additional, Tuju, James, additional, Fürle, Kristin, additional, Frank, Roland, additional, Chepsat, Emily, additional, Kapulu, Melissa C, additional, Study Team, CHMI-SIKA, additional, and Osier, Faith HA, additional

Published
2024
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3. Breadth of Fc-mediated effector function correlates with clinical immunity following human malaria challenge

Author

Nkumama, Irene N., primary, Ogwang, Rodney, additional, Odera, Dennis, additional, Musasia, Fauzia, additional, Mwai, Kennedy, additional, Nyamako, Lydia, additional, Murungi, Linda, additional, Tuju, James, additional, Fürle, Kristin, additional, Rosenkranz, Micha, additional, Kimathi, Rinter, additional, Njuguna, Patricia, additional, Hamaluba, Mainga, additional, Kapulu, Melissa C., additional, Frank, Roland, additional, and Osier, Faith H.A., additional

Published
2024
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9. Comparative performance of WANTAI ELISA for total immunoglobulin to receptor binding protein and an ELISA for IgG to spike protein in detecting SARS-CoV-2 antibodies in Kenyan populations

Author

Nyagwange, James, Kutima, Bernadette, Mwai, Kennedy, Karanja, Henry K., Gitonga, John N., Mugo, Daisy, Uyoga, Sophie, Tuju, James, Ochola-Oyier, Lynette I., Ndungu, Francis, Bejon, Philip, Agweyu, Ambrose, Adetifa, Ifedayo M.O., Scott, J.Anthony G., and Warimwe, George M.

Published
2022
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10. Antibody-Dependent Respiratory Burst against Plasmodium falciparum Merozoites in Individuals Living in an Area with Declining Malaria Transmission

Author

Mutemi, Doreen D., primary, Tuju, James, additional, Ogwang, Rodney, additional, Nyamako, Lydia, additional, Wambui, Kennedy M., additional, Cruz, Ivette R., additional, Villner, Pär, additional, Yman, Victor, additional, Kinyanjui, Samson M., additional, Rooth, Ingegerd, additional, Ngasala, Billy, additional, Färnert, Anna, additional, and Osier, Faith H. A., additional

Published
2024
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12. Antibody-Dependent Respiratory Burst against Plasmodium falciparum Merozoites in Individuals Living in an Area with Declining Malaria Transmission

Author

Mutemi, Doreen D., Tuju, James, Ogwang, Rodney, Nyamako, Lydia, Wambui, Kennedy M., Cruz, Ivette R., Villner, Pär, Yman, Victor, Kinyanjui, Samson M., Rooth, Ingegerd, Ngasala, Billy, Färnert, Anna, Osier, Faith H. A., Mutemi, Doreen D., Tuju, James, Ogwang, Rodney, Nyamako, Lydia, Wambui, Kennedy M., Cruz, Ivette R., Villner, Pär, Yman, Victor, Kinyanjui, Samson M., Rooth, Ingegerd, Ngasala, Billy, Färnert, Anna, and Osier, Faith H. A.

Abstract

Malaria transmission intensity affects the development of naturally acquired immunity to malaria. An absolute correlate measure of protection against malaria is lacking. However, antibody-mediated functions against Plasmodium falciparum correlate with protection against malaria. In children, antibody-mediated functions against P. falciparum decline with reduced exposure. It is unclear whether adults maintain antibody-mediated functions as malaria transmission declines. This study assessed antibody-dependent respiratory burst (ADRB) in individuals from an area with declining malaria transmission. In an age-matched analysis, we compare ADRB activity during high versus low malaria transmission periods. Age significantly predicted higher ADRB activity in the high (p < 0.001) and low (p < 0.001) malaria transmission periods. ADRB activity was higher during the high compared to the low malaria transmission period in older children and adults. Only older adults during the high malaria transmission period had their median ADRB activity above the ADRB cut-off. Ongoing P. falciparum infection influenced ADRB activity during the low (p = 0.01) but not the high (p = 0.29) malaria transmission period. These findings propose that naturally acquired immunity to P. falciparum is affected in children and adults as malaria transmission declines, implying that vaccines will be necessary to induce and maintain protection against malaria.

Published
2024
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13. Antibody-Dependent Respiratory Burst against Plasmodium falciparum Merozoites in Individuals Living in an Area with Declining Malaria Transmission

Author

Mutemi, Doreen David, primary, Tuju, James, additional, Ogwang, Rodney, additional, Nyamako, Lydia, additional, Wambui, Kennedy Mwai, additional, Cruz, Ivette Raices, additional, Villner, Pär, additional, Yman, Victor, additional, Kinyanjui, Samson Muchina, additional, Rooth, Ingegerd, additional, Ngasala, Billy, additional, Färnert, Anna, additional, and Osier, Faith, additional

Published
2024
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14. The landscape of paediatric infectious disease exposure in a rural sub-Saharan Africa setting in Kilifi, Kenya: longitudinal serological analysis over two decades and priorities for future vaccine development

Author

Foley, Deirdre F, primary, Chege, Timothy K, additional, Kabagenyi, Joyce, additional, McCarthy, Karen, additional, Gicheru, Elijah T, additional, Kibinge, Nelson, additional, Maina, Angela W, additional, Waeni, Jacqueline M, additional, Clemens, Ralf, additional, Clemens, Sue-Ann Costa, additional, Tuju, James, additional, and Sande, Charles J, additional

Published
2024
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15. Temporal trends of SARS-CoV-2 seroprevalence during the first wave of the COVID-19 epidemic in Kenya

Author

Adetifa, Ifedayo M. O., Uyoga, Sophie, Gitonga, John N., Mugo, Daisy, Otiende, Mark, Nyagwange, James, Karanja, Henry K., Tuju, James, Wanjiku, Perpetual, Aman, Rashid, Mwangangi, Mercy, Amoth, Patrick, Kasera, Kadondi, Ng’ang’a, Wangari, Rombo, Charles, Yegon, Christine, Kithi, Khamisi, Odhiambo, Elizabeth, Rotich, Thomas, Orgut, Irene, Kihara, Sammy, Bottomley, Christian, Kagucia, Eunice W., Gallagher, Katherine E., Etyang, Anthony, Voller, Shirine, Lambe, Teresa, Wright, Daniel, Barasa, Edwine, Tsofa, Benjamin, Bejon, Philip, Ochola-Oyier, Lynette I., Agweyu, Ambrose, Scott, J. Anthony G., and Warimwe, George M.

Published
2021
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16. Bi-isotype immunoglobulins enhance antibody-mediated neutrophil activity against Plasmodium falciparum parasites.

Author

Ogwang, Rodney, Murugu, Lewis, Nkumama, Irene N., Lydia Nyamako, Kai, Oscar, Mwai, Kennedy, Murungi, Linda, Idro, Richard, Bejon, Philip, Tuju, James, Kinyanjui, Sam Muchina, and Osier, Faith H. A.

Subjects
PLASMODIUM falciparum, IMMUNOGLOBULINS, NEUTROPHILS, REACTIVE oxygen species, MEROZOITES
Abstract

Background: Malaria remains a major global health priority, and monoclonal antibodies (mAbs) are emerging as potential new tools to support efforts to control the disease. Recent data suggest that Fc-dependent mechanisms of immunity are important mediators of protection against the blood stages of the infection, but few studies have investigated this in the context of mAbs. We aimed to isolate mAbs agnostic to cognate antigens that target whole merozoites and simultaneously induce potent neutrophil activity measured by the level of reactive oxygen species (ROS) production using an antibody-dependent respiratory burst (ADRB) assay. Methods: We used samples from semi-immune adults living in coastal Kenya to isolate mAbs that induce merozoite-specific ADRB activity. We then tested whether modifying the expressed IgG1 isotype to an IgG-IgA Fc region chimera would enhance the level of ADRB activity. Results: We isolated a panel of nine mAbs with specificity to whole merozoites. mAb J31 induced ADRB activity in a dose-dependent fashion. Compared to IgG1, our modified antibody IgG-IgA bi-isotype induced higher ADRB activity across all concentrations tested. Further, we observed a negative hook effect at high IgG1 mAb concentrations (i.e., >200 µg/mL), but this was reversed by Fc modification. We identified MSP3.5 as the potential cognate target of mAb J31. Conclusions: We demonstrate an approach to engineer mAbs with enhanced ADRB potency against blood-stage parasites. [ABSTRACT FROM AUTHOR]

Published
2024
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17. Antibody responses to recombinant oligopeptides of PfEMP1 isolated from children infected with Plasmodium falciparum

Author

Tuju, James Ogwindi

Subjects
616.9362
Abstract

Individuals living in malaria endemic areas acquire immunity against clinical disease through repeated encounters with the Plasmodium falciparum parasite during their childhood. As a result, the majority of severe cases of malaria are restricted to younger children. Naturally acquired immunity is thought to be partly mediated by antibodies directed at parasite derived antigens on the surface of red blood cells infected with mature forms of P. falciparum called variant surface antigens (VSA). Of these, P. falciparum erythrocyte membrane protein 1 is well characterized and associated with pathology and immune evasion. Sera obtained from young children living in endemic areas show limited recognition of PfEMP1 unlike immune adult sera that exhibit recognition of a wide range of PfEMP1. It is not yet clear whether this wide recognition of PfEMP1 by adult immune sera is exclusively a product of variant specific response accumulated over many exposures or whether it includes a cross-reactive response that recognizes a diverse set of antigens. Here, I have used the recombinant DBLα-tag region of PfEMP1 generated from parasites isolated from children infected with malaria to show that individuals develop a strong variant-specific response to their infecting isolate, accompanied by partly cross-reactive response. Based on antigenic and genetic similarities between different DBLα-tag variants, I identified potential epitope regions in this study that may account for the cross reactive responses. However, these appear to be conformational and predominantly lie towards the N-terminal end. Once verified, such regions may form good candidates for inclusion in a multi-epitope vaccine construct that affords broad protection against malaria.

Published
2013
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19. Characterization of a novel Plasmodium falciparum merozoite surface antigen and potential vaccine target

Author

Niaré, Karamoko, primary, Chege, Timothy, additional, Rosenkranz, Micha, additional, Mwai, Kennedy, additional, Saßmannshausen, Zoe, additional, Odera, Dennis, additional, Nyamako, Lydia, additional, Tuju, James, additional, Alfred, Tiono, additional, Waitumbi, John N., additional, Ogutu, Bernhards, additional, Sirima, Sodiomon B., additional, Awandare, Gordon, additional, Kouriba, Bourema, additional, Rayner, Julian C., additional, and Osier, Faith H. A., additional

Published
2023
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20. Molecular characterization of Plasmodium falciparum PHISTb proteins as potential targets of naturally-acquired immunity against malaria

Author

Isebe, Tony, Rono, Martin, Tuju, James, Bargul, Joel, Gichuki, Bonface, and Njunge, James

Subjects
animal diseases, parasitic diseases, Life Sciences, bacteria, chemical and pharmacologic phenomena, biochemical phenomena, metabolism, and nutrition, P. falciparum, PHISTb, P. falciparum, Immunity, PHISTb, naturally acquired immunity, antibody-antigen response
Abstract

Study focuses on evaluation of PHISTb antigens as targets of naturally acquired immunity against malaria. Recombinant PHISTb antigens were expressed in a bacterial expression system, purified and evaluated for antibody responses.

Published
2022
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21. Sero-surveillance for IgG to SARS-CoV-2 at antenatal care clinics in three Kenyan referral hospitals: Repeated cross-sectional surveys 2020–21

Author

Lucinde, Ruth K., primary, Mugo, Daisy, additional, Bottomley, Christian, additional, Karani, Angela, additional, Gardiner, Elizabeth, additional, Aziza, Rabia, additional, Gitonga, John N., additional, Karanja, Henry, additional, Nyagwange, James, additional, Tuju, James, additional, Wanjiku, Perpetual, additional, Nzomo, Edward, additional, Kamuri, Evans, additional, Thuranira, Kaugiria, additional, Agunda, Sarah, additional, Nyutu, Gideon, additional, Etyang, Anthony O., additional, Adetifa, Ifedayo M. O., additional, Kagucia, Eunice, additional, Uyoga, Sophie, additional, Otiende, Mark, additional, Otieno, Edward, additional, Ndwiga, Leonard, additional, Agoti, Charles N., additional, Aman, Rashid A., additional, Mwangangi, Mercy, additional, Amoth, Patrick, additional, Kasera, Kadondi, additional, Nyaguara, Amek, additional, Ng’ang’a, Wangari, additional, Ochola, Lucy B., additional, Namdala, Emukule, additional, Gaunya, Oscar, additional, Okuku, Rosemary, additional, Barasa, Edwine, additional, Bejon, Philip, additional, Tsofa, Benjamin, additional, Ochola-Oyier, L. Isabella, additional, Warimwe, George M., additional, Agweyu, Ambrose, additional, Scott, J. Anthony G., additional, and Gallagher, Katherine E., additional

Published
2022
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22. SARS-CoV-2 seroprevalence in three Kenyan health and demographic surveillance sites, December 2020-May 2021

Author

Etyang, Anthony O., primary, Adetifa, Ifedayo, additional, Omore, Richard, additional, Misore, Thomas, additional, Ziraba, Abdhalah K., additional, Ng’oda, Maurine A., additional, Gitau, Evelyn, additional, Gitonga, John, additional, Mugo, Daisy, additional, Kutima, Bernadette, additional, Karanja, Henry, additional, Toroitich, Monica, additional, Nyagwange, James, additional, Tuju, James, additional, Wanjiku, Perpetual, additional, Aman, Rashid, additional, Amoth, Patrick, additional, Mwangangi, Mercy, additional, Kasera, Kadondi, additional, Ng’ang’a, Wangari, additional, Akech, Donald, additional, Sigilai, Antipa, additional, Karia, Boniface, additional, Karani, Angela, additional, Voller, Shirine, additional, Agoti, Charles N., additional, Ochola-Oyier, Lynette I., additional, Otiende, Mark, additional, Bottomley, Christian, additional, Nyaguara, Amek, additional, Uyoga, Sophie, additional, Gallagher, Katherine, additional, Kagucia, Eunice W., additional, Onyango, Dickens, additional, Tsofa, Benjamin, additional, Mwangangi, Joseph, additional, Maitha, Eric, additional, Barasa, Edwine, additional, Bejon, Philip, additional, Warimwe, George M., additional, Scott, J. Anthony G., additional, and Agweyu, Ambrose, additional

Published
2022
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23. Epidemiology of COVID-19 infections on routine polymerase chain reaction (PCR) and serology testing in Coastal Kenya [version 1; peer review: 2 approved]

Author

Nyagwange, James, Ndwiga, Leonard, Muteru, Kelvin, Wamae, Kevin, Tuju, James, testing team, COVID, Kutima, Bernadette, Gitonga, John, Karanja, Henry, Mugo, Daisy, Kasera, Kadondi, Amoth, Patrick, Murunga, Nickson, Babu, Lawrence, Otieno, Edward, Githinji, George, Nokes, D. James, Tsofa, Benjamin, Orindi, Benedict, Barasa, Edwine, Warimwe, George, Agoti, Charles N., Bejon, Philip, and Ochola-Oyier, Lynette Isabella

Subjects
RB, QP, RA, RC
Abstract

Background: There are limited studies in Africa describing the epidemiology, clinical characteristics and serostatus of individuals tested for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. We tested routine samples from the Coastal part of Kenya between 17th March 2020 and 30th June 2021. Methods: SARS-CoV-2 infections identified using reverse transcription polymerase chain reaction (RT-PCR) and clinical surveillance data at the point of sample collection were used to classify as either symptomatic or asymptomatic. IgG antibodies were measured in sera samples, using a well validated in-house enzyme-linked immunosorbent assay (ELISA). Results: Mombasa accounted for 56.2% of all the 99,694 naso-pharyngeal/oro-pharyngeal swabs tested, and males constituted the majority tested (73.4%). A total of 7737 (7.7%) individuals were SARS-CoV-2 positive by RT-PCR. The majority (i.e., 92.4%) of the RT-PCR positive individuals were asymptomatic. Testing was dominated by mass screening and travellers, and even at health facility level 91.6% of tests were from individuals without symptoms. Out of the 97,124 tests from asymptomatic individuals 7,149 (7%) were positive and of the 2,568 symptomatic individuals 588 (23%) were positive. In total, 2458 serum samples were submitted with paired naso-pharyngeal/oro-pharyngeal samples and 45% of the RT-PCR positive samples and 20% of the RT-PCR negative samples were paired with positive serum samples. Symptomatic individuals had significantly higher antibody levels than asymptomatic individuals and become RT-PCR negative on repeat testing earlier than asymptomatic individuals. Conclusions: In conclusion, the majority of SARS-CoV-2 infections identified by routine testing in Coastal Kenya were asymptomatic. This reflects the testing practice of health services in Kenya, but also implies that asymptomatic infection is very common in the population. Symptomatic infection may be less common, or it may be that individuals do not present for testing when they have symptoms.

Published
2022

25. Characterization of a novel Plasmodium falciparum merozoite surface antigen and potential vaccine target.

Author

Karamoko Niaré, Chege, Timothy, Rosenkranz, Micha, Mwai, Kennedy, Saßmannshausen, Zoe, Odera, Dennis, Nyamako, Lydia, Tuju, James, Alfred, Tiono, Waitumbi, John N., Ogutu, Bernhards, Sirima, Sodiomon B., Awandare, Gordon, Kouriba, Bourema, Rayner, Julian C., and Osier, Faith H. A.

Subjects
CELL surface antigens, KILLER cells, SURFACE potential, PLASMODIUM falciparum, MALARIA vaccines, BABESIA
Abstract

Introduction: Detailed analyses of genetic diversity, antigenic variability, protein localization and immunological responses are vital for the prioritization of novel malaria vaccine candidates. Comprehensive approaches to determine the most appropriate antigen variants needed to provide broad protection are challenging and consequently rarely undertaken. Methods: Here, we characterized PF3D7_1136200, which we named Asparagine-Rich Merozoite Antigen (ARMA) based on the analysis of its sequence, localization and immunogenicity. We analyzed IgG and IgM responses against the common variants of ARMA in independent prospective cohort studies in Burkina Faso (N = 228), Kenya (N = 252) and Mali (N = 195) using a custom microarray, Div-KILCHIP. Results: We found a marked population structure between parasites from Africa and Asia. African isolates shared 34 common haplotypes, including a dominant pair although the overall selection pressure was directional (Tajima's D = -2.57; Fu and Li's F = -9.69; P < 0.02). ARMA was localized to the merozoite surface, IgG antibodies induced Fc-mediated degranulation of natural killer cells and strongly inhibited parasite growth in vitro. We found profound serological diversity, but IgG and IgM responses were highly correlated and a hierarchical clustering analysis identified only three major serogroups. Protective IgG and IgM antibodies appeared to target both cross-reactive and distinct epitopes across variants. However, combinations of IgG and IgM antibodies against selected variants were associated with complete protection against clinical episodes of malaria. Discussion: Our systematic strategy exploits genomic data to deduce the handful of antigen variants with the strongest potential to induce broad protection and may be broadly applicable to other complex pathogens for which effective vaccines remain elusive. [ABSTRACT FROM AUTHOR]

Published
2023
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26. Anti-merozoite antibodies induce natural killer cell effector function and are associated with immunity against malaria.

Author

Odera, Dennis O., Tuju, James, Mwai, Kennedy, Nkumama, Irene N., Fürle, Kristin, Chege, Timothy, Kimathi, Rinter, Diehl, Stefan, Musasia, Fauzia K., Rosenkranz, Micha, Njuguna, Patricia, Hamaluba, Mainga, Kapulu, Melissa C., Frank, Roland, Osier, Faith H. A., Abdi, Abdirahman I., Chi, Primus Che, de Laurent, Zaydah, Jao, Irene, and Kamuya, Dorcas

Subjects
KILLER cells, IMMUNE response, CELL physiology, MALARIA, FC receptors, ERYTHROCYTES, IMMUNITY
Abstract

Natural killer (NK) cells are potent immune effectors that can be activated via antibody-mediated Fc receptor engagement. Using multiparameter flow cytometry, we found that NK cells degranulate and release IFN-γ upon stimulation with antibody-opsonized Plasmodium falciparum merozoites. Antibody-dependent NK (Ab-NK) activity was largely strain transcending and enhanced invasion inhibition into erythrocytes. Ab-NK was associated with the successful control of parasitemia after experimental malaria challenge in African adults. In an independent cohort study in children, Ab-NK increased with age, was boosted by concurrent P. falciparum infections, and was associated with a lower risk of clinical episodes of malaria. Nine of the 14 vaccine candidates tested induced Ab-NK, including some less well-characterized antigens: P41, P113, MSP11, RHOPH3, and Pf_11363200. These data highlight an important role of Ab-NK activity in immunity against malaria and provide a potential mechanism for evaluating vaccine candidates. A different antibody angle: Natural killer (NK) cells have been shown to mediate immune responses against Plasmodium falciparum using multiple effector functions. Odera et al. now show that antibody-opsonized P. falciparum merozoites can induce NK cell degranulation and IFN-γ production. This response was not strain specific and reduced invasion of merozoites into uninfected red blood cells. Using a controlled human malaria infection study in adults, they observed that antibody-dependent NK (Ab-NK) cell activity correlated directly with the control of parasitemia. In addition, in a cohort of children living in an endemic malaria setting, Ab-NK frequency increased with age, was boosted during P. falciparum infection, and was linked to a reduced risk of clinical malaria. These findings highlight a key role for antibody-mediated NK cell responses during malaria. —CF [ABSTRACT FROM AUTHOR]

Published
2023
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27. High-throughput profiling of natural acquired humoral immune response to a large panel of known and novel full length ectodomain P. falciparum merozoite vaccine candidates under reduced malaria transmission

Author

Ndungu, Duncan Ndegwa, primary, Tuju, James, additional, Chepsat, Emily, additional, Mwai, Rinter, additional, Mwai, Kennedy, additional, Nyamako, Lydia, additional, Mosobo, Moses, additional, Deme, Awa B., additional, Dieye, Baba, additional, Ndiaye, Ibrahima Mbaye, additional, Sy, Mouhamad, additional, Diallo, Mamadou Alpha, additional, Diedhiou, Younous, additional, Mbaye, Amadou Moctar, additional, Wirth, Dyann, additional, Ndiaye, Daouda, additional, Osier, Faith, additional, and Bei, Amy K., additional

Published
2022
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28. Additional file 5 of Characterization of Anopheles gambiae D7 salivary proteins as markers of human���mosquito bite contact

Author

Oseno, Brenda, Marura, Faith, Ogwang, Rodney, Muturi, Martha, Njunge, James, Nkumama, Irene, Mwakesi, Robert, Mwai, Kennedy, Rono, Martin K., Mwakubambanya, Ramadhan, Osier, Faith, and Tuju, James

Subjects
parasitic diseases
Abstract

Additional file 5: Table S3. Kruskal���Wallis test comparing differences in responses to salivary gland antigens between the sites.

Published
2022
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30. Additional file 5 of Controlled human malaria infection (CHMI) outcomes in Kenyan adults is associated with prior history of malaria exposure and anti-schizont antibody response

Author

Kapulu, Melissa C., Kimani, Domtila, Njuguna, Patricia, Hamaluba, Mainga, Otieno, Edward, Kimathi, Rinter, Tuju, James, and Sim, B. Kim Lee

Subjects
fungi, parasitic diseases
Abstract

Additional file 5: Table S1. Sub-group analysis of qPCR outcome in relation to anti-schizont antibody responses. Table S2. Parametric analysis of qPCR parameters with anti-schizont antibody responses and location.

Published
2022
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31. Additional file 4 of Characterization of Anopheles gambiae D7 salivary proteins as markers of human���mosquito bite contact

Author

Oseno, Brenda, Marura, Faith, Ogwang, Rodney, Muturi, Martha, Njunge, James, Nkumama, Irene, Mwakesi, Robert, Mwai, Kennedy, Rono, Martin K., Mwakubambanya, Ramadhan, Osier, Faith, and Tuju, James

Subjects
parasitic diseases
Abstract

Additional file 4: Table S2. Identification of the recombinant mosquito salivary antigens by mass spectrometry.

Published
2022
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32. Additional file 3 of Characterization of Anopheles gambiae D7 salivary proteins as markers of human���mosquito bite contact

Author

Oseno, Brenda, Marura, Faith, Ogwang, Rodney, Muturi, Martha, Njunge, James, Nkumama, Irene, Mwakesi, Robert, Mwai, Kennedy, Rono, Martin K., Mwakubambanya, Ramadhan, Osier, Faith, and Tuju, James

Abstract

Additional file 3: Text S1. Sample preparation, liquid chromatography-tandem mass spectrometry (LC���MS/MS) analysis and antigen validation.

Published
2022
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33. Additional file 6 of Characterization of Anopheles gambiae D7 salivary proteins as markers of human���mosquito bite contact

Author

Oseno, Brenda, Marura, Faith, Ogwang, Rodney, Muturi, Martha, Njunge, James, Nkumama, Irene, Mwakesi, Robert, Mwai, Kennedy, Rono, Martin K., Mwakubambanya, Ramadhan, Osier, Faith, and Tuju, James

Abstract

Additional file 6: Table S4. Dunn test post hoc pairwise multiple comparisons for differences in responses to salivary gland antigens between the sites.

Published
2022
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34. Breadth of Fc-Mediated Effector Function Delineates Grades of Clinical Immunity Following Human Malaria Challenge

Author

Nkumama, Irene, primary, Odero, Dennis, additional, Musasia, Fauzia, additional, Wambui, Kennedy Mwai, additional, Nyamako, Lydia, additional, Murungi, Linda, additional, Tuju, James, additional, Fürle, Kristin, additional, Rosenkranz, Micha, additional, Kimathi, Rinter, additional, Njuguna, Patricia, additional, Hamaluba, Mainga, additional, Kapulu, Melissa C., additional, Frank, Roland, additional, Team, CHMI-SIKA Study, additional, and Osier, Faith Hope Among&apos;in, additional

Published
2022
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35. Anti-Severe Acute Respiratory Syndrome Coronavirus 2 Immunoglobulin G Antibody Seroprevalence Among Truck Drivers and Assistants in Kenya

Author

Kagucia, E Wangeci, Gitonga, John N, Kalu, Catherine, Ochomo, Eric, Ochieng, Benard, Kuya, Nickline, Karani, Angela, Nyagwange, James, Karia, Boniface, Mugo, Daisy, Karanja, Henry K, Tuju, James, Mutiso, Agnes, Maroko, Hosea, Okubi, Lucy, Maitha, Eric, Ajuck, Hossan, Mukabi, David, Moracha, Wycliffe, Bulimu, David, Andanje, Nelson, Aman, Rashid, Mwangangi, Mercy, Amoth, Patrick, Kasera, Kadondi, Ng'ang'a, Wangari, Nyaguara, Amek, Voller, Shirine, Otiende, Mark, Bottomley, Christian, Agoti, Charles N, Ochola-Oyier, Lynette I, Adetifa, Ifedayo MO, Etyang, Anthony O, Gallagher, Katherine E, Uyoga, Sophie, Barasa, Edwine, Bejon, Philip, Tsofa, Benjamin, Agweyu, Ambrose, Warimwe, George M, Scott, J Anthony G, and Magarini Sub-County TDA SARS-CoV-2 Serosurveillance Team, The Bu

Subjects
viruses, virus diseases, human activities
Abstract

In October 2020, anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) immunoglobulin G seroprevalence among truck drivers and their assistants (TDA) in Kenya was 42.3%, higher than among healthcare workers and blood donors. Truck drivers and their assistants transport essential supplies during the coronavirus disease 2019 pandemic, placing them at increased risk of being infected and of transmitting SARS-CoV-2 over a wide geographical area.

Published
2021

37. Prevalence of SARS-CoV-2 Antibodies from a one-year National Serosurveillance of Kenyan Blood Transfusion Donors

Author

Uyoga, Sophie, primary, Adetifa, Ifedayo M.O., additional, Otiende, Mark, additional, Gitonga, John N., additional, Mugo, Daisy, additional, Nyagwange, James, additional, Karanja, Henry K., additional, Tuju, James, additional, Makale, Johnstone, additional, Aman, Rashid, additional, Mwangangi, Mercy, additional, Amoth, Patrick, additional, Kasera, Kadondi, additional, Ng’ang’a, Wangari, additional, Kilonzo, Nduku, additional, Chege, Evelynn, additional, Yegon, Christine, additional, Odhiambo, Elizabeth, additional, Rotich, Thomas, additional, Orgut, Irene, additional, Kihara, Sammy, additional, Bottomley, Christian, additional, Kagucia, Eunice W., additional, Gallagher, Katherine E., additional, Etyang, Anthony, additional, Voller, Shirine, additional, Lambe, Teresa, additional, Wright, Daniel, additional, Barasa, Edwine, additional, Tsofa, Benjamin, additional, Mwangangi, Joseph, additional, Bejon, Philip, additional, Ochola-Oyier, Lynette I., additional, Warimwe, George M., additional, Agweyu, Ambrose, additional, and Scott, J. Anthony G., additional

Published
2021
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39. Seroprevalence of Antibodies to Severe Acute Respiratory Syndrome Coronavirus 2 Among Healthcare Workers in Kenya

Author

Etyang, Anthony O, primary, Lucinde, Ruth, additional, Karanja, Henry, additional, Kalu, Catherine, additional, Mugo, Daisy, additional, Nyagwange, James, additional, Gitonga, John, additional, Tuju, James, additional, Wanjiku, Perpetual, additional, Karani, Angela, additional, Mutua, Shadrack, additional, Maroko, Hosea, additional, Nzomo, Eddy, additional, Maitha, Eric, additional, Kamuri, Evanson, additional, Kaugiria, Thuranira, additional, Weru, Justus, additional, Ochola, Lucy B, additional, Kilimo, Nelson, additional, Charo, Sande, additional, Emukule, Namdala, additional, Moracha, Wycliffe, additional, Mukabi, David, additional, Okuku, Rosemary, additional, Ogutu, Monicah, additional, Angujo, Barrack, additional, Otiende, Mark, additional, Bottomley, Christian, additional, Otieno, Edward, additional, Ndwiga, Leonard, additional, Nyaguara, Amek, additional, Voller, Shirine, additional, Agoti, Charles N, additional, Nokes, David James, additional, Ochola-Oyier, Lynette Isabella, additional, Aman, Rashid, additional, Amoth, Patrick, additional, Mwangangi, Mercy, additional, Kasera, Kadondi, additional, Ng’ang’a, Wangari, additional, Adetifa, Ifedayo M O, additional, Wangeci Kagucia, E, additional, Gallagher, Katherine, additional, Uyoga, Sophie, additional, Tsofa, Benjamin, additional, Barasa, Edwine, additional, Bejon, Philip, additional, Scott, J Anthony G, additional, Agweyu, Ambrose, additional, and Warimwe, George M, additional

Published
2021
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40. Seroprevalence of Antibodies to SARS-CoV-2 among Health Care Workers in Kenya

Author

Etyang, Anthony O., primary, Lucinde, Ruth, additional, Karanja, Henry, additional, Kalu, Catherine, additional, Mugo, Daisy, additional, Nyagwange, James, additional, Gitonga, John, additional, Tuju, James, additional, Wanjiku, Perpetual, additional, Karani, Angela, additional, Mutua, Shadrack, additional, Maroko, Hosea, additional, Nzomo, Eddy, additional, Maitha, Eric, additional, Kamuri, Evanson, additional, Kaugiria, Thuranira, additional, Weru, Justus, additional, Ochola, Lucy B., additional, Kilimo, Nelson, additional, Charo, Sande, additional, Emukule, Namdala, additional, Moracha, Wycliffe, additional, Mukabi, David, additional, Okuku, Rosemary, additional, Ogutu, Monicah, additional, Angujo, Barrack, additional, Otiende, Mark, additional, Bottomley, Christian, additional, Otieno, Edward, additional, Ndwiga, Leonard, additional, Nyaguara, Amek, additional, Voller, Shirine, additional, Agoti, Charles, additional, Nokes, David James, additional, Ochola-Oyier, Lynette Isabella, additional, Aman, Rashid, additional, Amoth, Patrick, additional, Mwangangi, Mercy, additional, Kasera, Kadondi, additional, Ng’ang’a, Wangari, additional, Adetifa, Ifedayo, additional, Kagucia, E. Wangeci, additional, Gallagher, Katherine, additional, Uyoga, Sophie, additional, Tsofa, Benjamin, additional, Barasa, Edwine, additional, Bejon, Philip, additional, Scott, J. Anthony G., additional, Agweyu, Ambrose, additional, and Warimwe, George, additional

Published
2021
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41. Seroprevalence of anti-SARS-CoV-2 IgG antibodies among truck drivers and assistants in Kenya

Author

Kagucia, E Wangeci, primary, Gitonga, John N, additional, Kalu, Catherine, additional, Ochomo, Eric, additional, Ochieng, Benard, additional, Kuya, Nickline, additional, Karani, Angela, additional, Nyagwange, James, additional, Karia, Boniface, additional, Mugo, Daisy, additional, Karanja, Henry K, additional, Tuju, James, additional, Mutiso, Agnes, additional, Maroko, Hosea, additional, Okubi, Lucy, additional, Maitha, Eric, additional, Ajuck, Hossan, additional, Bogita, Mary, additional, Mudindi, Richmond, additional, Mukabi, David, additional, Moracha, Wycliffe, additional, Bulimu, David, additional, Andanje, Nelson, additional, Shiraku, Evans, additional, Okuku, Rosemary, additional, Ogutu, Monicah, additional, Aman, Rashid, additional, Mwangangi, Mercy, additional, Amoth, Patrick, additional, Kasera, Kadondi, additional, Ng’ang’a, Wangari, additional, Mariga, Rodgers, additional, Munabi, Tobias, additional, Ramadhan, Susan M, additional, Mwikali, Janet, additional, Nasike, Rose, additional, Andera, Cornelius, additional, Nechesa, Roselyne, additional, Kiplagat, Benson K, additional, Omengo, Julius, additional, Oteba, Simon, additional, Mwangi, Arthur, additional, Mkanyi, Dorcas, additional, Karisa, George, additional, Migosi, Judith K, additional, Msili, Patrick, additional, Mwambire, Samson, additional, Boniface, Anthony M, additional, Nyaguara, Amek, additional, Voller, Shirine, additional, Otiende, Mark, additional, Bottomley, Christian, additional, Agoti, Charles N, additional, Ochola-Oyier, Lynette I, additional, Adetifa, Ifedayo M O, additional, Etyang, Anthony O, additional, Gallagher, Katherine E, additional, Uyoga, Sophie, additional, Barasa, Edwine, additional, Bejon, Philip, additional, Tsofa, Benjamin, additional, Agweyu, Ambrose, additional, Warimwe, George M, additional, and Scott, J Anthony G, additional

Published
2021
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42. Temporal trends of SARS-CoV-2 seroprevalence in transfusion blood donors during the first wave of the COVID-19 epidemic in Kenya

Author

Adetifa, Ifedayo M.O., primary, Uyoga, Sophie, additional, Gitonga, John N., additional, Mugo, Daisy, additional, Otiende, Mark, additional, Nyagwange, James, additional, Karanja, Henry K., additional, Tuju, James, additional, Wanjiku, Perpetual, additional, Aman, Rashid, additional, Mwangangi, Mercy, additional, Amoth, Patrick, additional, Kasera, Kadondi, additional, Ng’ang’a, Wangari, additional, Rombo, Charles, additional, Yegon, Christine, additional, Kithi, Khamisi, additional, Odhiambo, Elizabeth, additional, Rotich, Thomas, additional, Orgut, Irene, additional, Kihara, Sammy, additional, Bottomley, Christian, additional, Kagucia, Eunice W., additional, Gallagher, Katherine E., additional, Etyang, Anthony, additional, Voller, Shirine, additional, Lambe, Teresa, additional, Wright, Daniel, additional, Barasa, Edwine, additional, Tsofa, Benjamin, additional, Bejon, Philip, additional, Ochola-Oyier, Lynette I., additional, Agweyu, Ambrose, additional, Scott, J. Anthony G., additional, and Warimwe, George M., additional

Published
2021
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43. Seroprevalence of anti–SARS-CoV-2 IgG antibodies in Kenyan blood donors

Author

Uyoga, Sophie, primary, Adetifa, Ifedayo M. O., additional, Karanja, Henry K., additional, Nyagwange, James, additional, Tuju, James, additional, Wanjiku, Perpetual, additional, Aman, Rashid, additional, Mwangangi, Mercy, additional, Amoth, Patrick, additional, Kasera, Kadondi, additional, Ng’ang’a, Wangari, additional, Rombo, Charles, additional, Yegon, Christine, additional, Kithi, Khamisi, additional, Odhiambo, Elizabeth, additional, Rotich, Thomas, additional, Orgut, Irene, additional, Kihara, Sammy, additional, Otiende, Mark, additional, Bottomley, Christian, additional, Mupe, Zonia N., additional, Kagucia, Eunice W., additional, Gallagher, Katherine E., additional, Etyang, Anthony, additional, Voller, Shirine, additional, Gitonga, John N., additional, Mugo, Daisy, additional, Agoti, Charles N., additional, Otieno, Edward, additional, Ndwiga, Leonard, additional, Lambe, Teresa, additional, Wright, Daniel, additional, Barasa, Edwine, additional, Tsofa, Benjamin, additional, Bejon, Philip, additional, Ochola-Oyier, Lynette I., additional, Agweyu, Ambrose, additional, Scott, J. Anthony G., additional, and Warimwe, George M., additional

Published
2021
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44. Distinct kinetics in antibody responses to 111 Plasmodium falciparum antigens identifies novel serological markers of recent malaria exposure

Author

Yman, Victor, primary, Tuju, James, additional, White, Michael T, additional, Kamuyu, Gathoni, additional, Mwai, Kennedy, additional, Kibinge, Nelson, additional, Asghar, Muhammad, additional, Sundling, Christopher, additional, Sonden, Klara, additional, Murungi, Linda, additional, Kiboi, Daniel, additional, Kimathi, Rinther, additional, Chege, Timothy, additional, Chepsat, Emily, additional, Kiyuka, Patience, additional, Nyamako, Lydia, additional, Osier, Faith H A, additional, and Farnert, Anna, additional

Published
2020
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45. Systematic Serological Diversity of a Putative Malaria Vaccine Candidate and Broad Protection

Author

Niare, Karamoko, primary, Chege, Timothy, additional, Mwai, Kennedy, additional, Tuju, James, additional, Nyamako, Lydia, additional, Rosenkranz, Micha, additional, Tiono, Alfred, additional, Waitumbi, John, additional, Ogutu, Bernhards, additional, Sirima, Sodiomon, additional, Awandare, Gordon, additional, Kouriba, Bourema, additional, Rayner, Julian, additional, and Osier, Faith, additional

Published
2020
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47. Seroprevalence of anti-SARS-CoV-2 IgG antibodies in Kenyan blood donors

Author

Uyoga, Sophie, primary, M.O. Adetifa, Ifedayo, additional, Karanja, Henry K., additional, Nyagwange, James, additional, Tuju, James, additional, Wanjiku, Perpetual, additional, Aman, Rashid, additional, Mwangangi, Mercy, additional, Amoth, Patrick, additional, Kasera, Kadondi, additional, Ng’ang’a, Wangari, additional, Rombo, Charles, additional, Yegon, Christine, additional, Kithi, Khamisi, additional, Odhiambo, Elizabeth, additional, Rotich, Thomas, additional, Orgut, Irene, additional, Kihara, Sammy, additional, Otiende, Mark, additional, Bottomley, Christian, additional, N. Mupe, Zonia, additional, W. Kagucia, Eunice, additional, E Gallagher, Katherine, additional, Etyang, Anthony, additional, Voller, Shirine, additional, N. Gitonga, John, additional, Mugo, Daisy, additional, N. Agoti, Charles, additional, Otieno, Edward, additional, Ndwiga, Leonard, additional, Lambe, Teresa, additional, Wright, Daniel, additional, Barasa, Edwine, additional, Tsofa, Benjamin, additional, Bejon, Philip, additional, I. Ochola-Oyier, Lynette, additional, Agweyu, Ambrose, additional, Scott, J. Anthony G., additional, and Warimwe, George M., additional

Published
2020
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48. Seroprevalence of Antibodies to Severe Acute Respiratory Syndrome Coronavirus 2 Among Healthcare Workers in Kenya.

Author

Etyang, Anthony O, Lucinde, Ruth, Karanja, Henry, Kalu, Catherine, Mugo, Daisy, Nyagwange, James, Gitonga, John, Tuju, James, Wanjiku, Perpetual, Karani, Angela, Mutua, Shadrack, Maroko, Hosea, Nzomo, Eddy, Maitha, Eric, Kamuri, Evanson, Kaugiria, Thuranira, Weru, Justus, Ochola, Lucy B, Kilimo, Nelson, and Charo, Sande

Subjects
RESEARCH, SEROPREVALENCE, PUBLIC health surveillance, SARS-CoV-2, IMMUNOGLOBULINS, CONFIDENCE intervals, MULTIVARIATE analysis, MATHEMATICAL models, ENZYME-linked immunosorbent assay, THEORY, VIRAL antibodies, PROBABILITY theory
Abstract

Background Few studies have assessed the seroprevalence of antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) among healthcare workers (HCWs) in Africa. We report findings from a survey among HCWs in 3 counties in Kenya. Methods We recruited 684 HCWs from Kilifi (rural), Busia (rural), and Nairobi (urban) counties. The serosurvey was conducted between 30 July and 4 December 2020. We tested for immunoglobulin G antibodies to SARS-CoV-2 spike protein, using enzyme-linked immunosorbent assay. Assay sensitivity and specificity were 92.7 (95% CI, 87.9-96.1) and 99.0% (95% CI, 98.1-99.5), respectively. We adjusted prevalence estimates, using bayesian modeling to account for assay performance. Results The crude overall seroprevalence was 19.7% (135 of 684). After adjustment for assay performance, seroprevalence was 20.8% (95% credible interval, 17.5%–24.4%). Seroprevalence varied significantly (P <.001) by site: 43.8% (95% credible interval, 35.8%–52.2%) in Nairobi, 12.6% (8.8%–17.1%) in Busia and 11.5% (7.2%–17.6%) in Kilifi. In a multivariable model controlling for age, sex, and site, professional cadre was not associated with differences in seroprevalence. Conclusion These initial data demonstrate a high seroprevalence of antibodies to SARS-CoV-2 among HCWs in Kenya. There was significant variation in seroprevalence by region, but not by cadre. [ABSTRACT FROM AUTHOR]

Published
2022
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50. KILchip v1.0: A Novel Plasmodium falciparum Merozoite Protein Microarray to Facilitate Malaria Vaccine Candidate Prioritization

Author

Kamuyu, Gathoni, primary, Tuju, James, additional, Kimathi, Rinter, additional, Mwai, Kennedy, additional, Mburu, James, additional, Kibinge, Nelson, additional, Chong Kwan, Marisa, additional, Hawkings, Sam, additional, Yaa, Reuben, additional, Chepsat, Emily, additional, Njunge, James M., additional, Chege, Timothy, additional, Guleid, Fatuma, additional, Rosenkranz, Micha, additional, Kariuki, Christopher K., additional, Frank, Roland, additional, Kinyanjui, Samson M., additional, Murungi, Linda M., additional, Bejon, Philip, additional, Färnert, Anna, additional, Tetteh, Kevin K. A., additional, Beeson, James G., additional, Conway, David J., additional, Marsh, Kevin, additional, Rayner, Julian C., additional, and Osier, Faith H. A., additional

Published
2018
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