177 results on '"Tsuyoshi Mori"'
Search Results
2. Pentosan polysulfate induces low-level persistent prion infection keeping measurable seeding activity without PrP-res detection in Fukuoka-1 infected cell cultures
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Hanae Takatsuki, Morikazu Imamura, Tsuyoshi Mori, and Ryuichiro Atarashi
- Subjects
Medicine ,Science - Abstract
Abstract Each prion strain has its own characteristics and the efficacy of anti-prion drugs varies. Screening of prion disease therapeutics is typically evaluated by measuring amounts of protease-resistant prion protein (PrP-res). However, it remains unclear whether such measurements correlate with seeding activity, which is evaluated by real-time quaking-induced conversion (RT-QuIC). In this study, the effects of anti-prion compounds pentosan polysulfate (PPS), Congo red, and alprenolol were measured in N2a58 cells infected with Fukuoka-1 (FK1) or 22L strain. The compounds abolished PrP-res and seeding activity, except for N2a58/FK1 treated with PPS. Interestingly, the seeding activity of N2a58/FK1, which was reduced in the presence of PPS, was not lost and remained at low levels. However, upon removal of PPS, both were gradually restored to their original levels. These results indicate that low-level persistent prion infection keeping measurable seeding activity is induced by PPS in a strain-dependent manner. Furthermore, for protein misfolding cyclic amplification (PMCA), the anti-prion effect of PPS decreased in FK1 compared to 22L, suggesting that the differences occur at the level of the direct conversion. Our findings demonstrate that the advantages of RT-QuIC and PMCA can be exploited for more accurate assessment of therapeutic drug screening, reflecting strain differences.
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- 2022
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3. Mechanosensory trichome cells evoke a mechanical stimuli–induced immune response in Arabidopsis thaliana
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Mamoru Matsumura, Mika Nomoto, Tomotaka Itaya, Yuri Aratani, Mizuki Iwamoto, Takakazu Matsuura, Yuki Hayashi, Tsuyoshi Mori, Michael J. Skelly, Yoshiharu Y. Yamamoto, Toshinori Kinoshita, Izumi C. Mori, Takamasa Suzuki, Shigeyuki Betsuyaku, Steven H. Spoel, Masatsugu Toyota, and Yasuomi Tada
- Subjects
Science - Abstract
Plant immunity can be induced by pathogen signals or environmental cues. Here, the authors show that plant leaves use trichomes to sense incoming raindrops and trigger basal defence responses to protect against subsequent microbial infection.
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- 2022
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4. The complete mitochondrial genome of a bagrid catfish, Tachysurus nudiceps, and its phylogenetic implications for the classification of the bagrid genera
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Syuri Hashimoto, Ryosuke Kakehashi, Tsuyoshi Mori, Chiaki Kambayashi, Shigefumi Kanao, and Atsushi Kurabayashi
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mitogenome ,next-generation sequencing ,tachysurus nudiceps ,bagridae ,Genetics ,QH426-470 - Abstract
The complete sequence of the mitochondrial genome (mitogenome) of Tachysurus nudiceps (family Bagridae; order Siluriformes) was determined using next-generation sequencing. The composition of its mitogenome is the same as that observed in most other vertebrates and consists of 37 genes, an L-strand replication origin and a control region. As in previous studies, our phylogenetic analyses revealed that many of the bagrid genera are not monophyletic, emphasizing the necessity for reviewing and revising the taxonomy of this family at the genus level.
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- 2022
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5. Suppression of MYC transcription activators by the immune cofactor NPR1 fine-tunes plant immune responses
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Mika Nomoto, Michael J. Skelly, Tomotaka Itaya, Tsuyoshi Mori, Takamasa Suzuki, Tomonao Matsushita, Mutsutomo Tokizawa, Keiko Kuwata, Hitoshi Mori, Yoshiharu Y. Yamamoto, Tetsuya Higashiyama, Hironaka Tsukagoshi, Steven H. Spoel, and Yasuomi Tada
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plant immunity ,salicylic acid ,jasmonic acid ,coronatine ,biotroph ,necrotroph ,Biology (General) ,QH301-705.5 - Abstract
Summary: Plants tailor immune responses to defend against pathogens with different lifestyles. In this process, antagonism between the immune hormones salicylic acid (SA) and jasmonic acid (JA) optimizes transcriptional signatures specifically to the attacker encountered. Antagonism is controlled by the transcription cofactor NPR1. The indispensable role of NPR1 in activating SA-responsive genes is well understood, but how it functions as a repressor of JA-responsive genes remains unclear. Here, we demonstrate that SA-induced NPR1 is recruited to JA-responsive promoter regions that are co-occupied by a JA-induced transcription complex consisting of the MYC2 activator and MED25 Mediator subunit. In the presence of SA, NPR1 physically associates with JA-induced MYC2 and inhibits transcriptional activation by disrupting its interaction with MED25. Importantly, NPR1-mediated inhibition of MYC2 is a major immune mechanism for suppressing pathogen virulence. Thus, NPR1 orchestrates the immune transcriptome not only by activating SA-responsive genes but also by acting as a corepressor of JA-responsive MYC2.
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- 2021
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6. Ethanolamine Is a New Anti-Prion Compound
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Keiji Uchiyama, Hideyuki Hara, Junji Chida, Agriani Dini Pasiana, Morikazu Imamura, Tsuyoshi Mori, Hanae Takatsuki, Ryuichiro Atarashi, and Suehiro Sakaguchi
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prions ,prion protein ,protein misfolding ,neurodegeneration ,ethanolamine ,therapy ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Prion diseases are a group of fatal neurodegenerative disorders caused by accumulation of proteinaceous infectious particles, or prions, which mainly consist of the abnormally folded, amyloidogenic prion protein, designated PrPSc. PrPSc is produced through conformational conversion of the cellular isoform of prion protein, PrPC, in the brain. To date, no effective therapies for prion diseases have been developed. In this study, we incidentally noticed that mouse neuroblastoma N2a cells persistently infected with 22L scrapie prions, termed N2aC24L1-3 cells, reduced PrPSc levels when cultured in advanced Dulbecco’s modified eagle medium (DMEM) but not in classic DMEM. PrPC levels remained unchanged in prion-uninfected parent N2aC24 cells cultured in advanced DMEM. These results suggest that advanced DMEM may contain an anti-prion compound(s). We then successfully identified ethanolamine in advanced DMEM has an anti-prion activity. Ethanolamine reduced PrPSc levels in N2aC24L1-3 cells, but not PrPC levels in N2aC24 cells. Also, oral administration of ethanolamine through drinking water delayed prion disease in mice intracerebrally inoculated with RML scrapie prions. These results suggest that ethanolamine could be a new anti-prion compound.
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- 2021
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7. Prion-Seeding Activity Is widely Distributed in Tissues of Sporadic Creutzfeldt-Jakob Disease Patients
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Hanae Takatsuki, PhD, Takayuki Fuse, PhD, Takehiro Nakagaki, MD, PhD, Tsuyoshi Mori, PhD, Ban Mihara, MD, PhD, Masaki Takao, MD, PhD, Yasushi Iwasaki, MD, PhD, Mari Yoshida, MD, PhD, Shigeo Murayama, MD, PhD, Ryuichiro Atarashi, MD, PhD, Noriyuki Nishida, MD, PhD, and Katsuya Satoh, MD, PhD
- Subjects
Prion ,Prion-seeding activity ,SD50 ,Non-neural tissue ,Creutzfeldt-Jakob disease ,Medicine ,Medicine (General) ,R5-920 - Abstract
Human prion diseases are neurodegenerative disorders caused by abnormally folded prion proteins in the central nervous system. These proteins can be detected using the quaking-induced conversion assay. Compared with other bioassays, this assay is extremely sensitive and was used in the present study to determine prion distribution in sporadic Creutzfeldt-Jakob disease patients at autopsy. Although infectivity of the sporadic form is thought to be restricted within the central nervous system, results showed that prion-seeding activities reach 106/g from a 50% seeding dose in non-neuronal tissues, suggesting that prion-seeding activity exists in non-neural organs, and we suggested that non-neural tissues of 106/g SD50 did not exist the infectivity.
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- 2016
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8. Sequential Washing with Electrolyzed Alkaline and Acidic Water Effectively Removes Pathogens from Metal Surfaces.
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Yuichiro Nakano, Norihiko Akamatsu, Tsuyoshi Mori, Kazunori Sano, Katsuya Satoh, Takeshi Nagayasu, Yoshiaki Miyoshi, Tomomi Sugio, Hideyuki Sakai, Eiji Sakae, Kazuko Ichimiya, Masahisa Hamada, Takehisa Nakayama, Yuhzo Fujita, Katsunori Yanagihara, and Noriyuki Nishida
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Medicine ,Science - Abstract
Removal of pathogenic organisms from reprocessed surgical instruments is essential to prevent iatrogenic infections. Some bacteria can make persistent biofilms on medical devices. Contamination of non-disposable equipment with prions also represents a serious risk to surgical patients. Efficient disinfection of prions from endoscopes and other instruments such as high-resolution cameras remains problematic because these instruments do not tolerate aggressive chemical or heat treatments. Herein, we develop a new washing system that uses both the alkaline and acidic water produced by electrolysis. Electrolyzed acidic water, containing HCl and HOCl as active substances, has been reported to be an effective disinfectant. A 0.15% NaCl solution was electrolyzed and used immediately to wash bio-contaminated stainless steel model systems with alkaline water (pH 11.9) with sonication, and then with acidic water (pH 2.7) without sonication. Two bacterial species (Staphylococcus aureus and Pseudomonas aeruginosa) and a fungus (Candida albicans) were effectively removed or inactivated by the washing process. In addition, this process effectively removed or inactivated prions from the stainless steel surfaces. This washing system will be potentially useful for the disinfection of clinical devices such as neuroendoscopes because electrolyzed water is gentle to both patients and equipment and is environmentally sound.
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- 2016
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9. Rapid and Quantitative Assay of Amyloid-Seeding Activity in Human Brains Affected with Prion Diseases.
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Hanae Takatsuki, Katsuya Satoh, Kazunori Sano, Takayuki Fuse, Takehiro Nakagaki, Tsuyoshi Mori, Daisuke Ishibashi, Ban Mihara, Masaki Takao, Yasushi Iwasaki, Mari Yoshida, Ryuichiro Atarashi, and Noriyuki Nishida
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Medicine ,Science - Abstract
The infectious agents of the transmissible spongiform encephalopathies are composed of amyloidogenic prion protein, PrPSc. Real-time quaking-induced conversion can amplify very small amounts of PrPSc seeds in tissues/body fluids of patients or animals. Using this in vitro PrP-amyloid amplification assay, we quantitated the seeding activity of affected human brains. End-point assay using serially diluted brain homogenates of sporadic Creutzfeldt-Jakob disease patients demonstrated that 50% seeding dose (SD50) is reached approximately 10(10)/g brain (values varies 10(8.79-10.63)/g). A genetic case (GSS-P102L) yielded a similar level of seeding activity in an autopsy brain sample. The range of PrPSc concentrations in the samples, determined by dot-blot assay, was 0.6-5.4 μg/g brain; therefore, we estimated that 1 SD50 unit was equivalent to 0.06-0.27 fg of PrPSc. The SD50 values of the affected brains dropped more than three orders of magnitude after autoclaving at 121°C. This new method for quantitation of human prion activity provides a new way to reduce the risk of iatrogenic prion transmission.
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- 2015
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10. Association between reduction of plasma adiponectin levels and risk of bacterial infection after gastric cancer surgery.
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Hiroshi Yamamoto, Kazuhisa Maeda, Yoshitaka Uji, Hiroshi Tsuchihashi, Tsuyoshi Mori, Tomoharu Shimizu, Yoshihiro Endo, Aya Kadota, Katsuyuki Miura, Yusuke Koga, Toshinori Ito, and Tohru Tani
- Subjects
Medicine ,Science - Abstract
BACKGROUND AND PURPOSE: Infections are important causes of postoperative morbidity after gastric surgery; currently, no factors have been identified that can predict postoperative infection. Adiponectin (ADN) mediates energy metabolism and functions as an immunomodulator. Perioperative ADN levels and perioperative immune functioning could be mutually related. Here we evaluated a potential biological marker to reliably predict the incidence of postoperative infections to prevent such comorbidities. METHODS: We analyzed 150 consecutive patients who underwent elective gastric cancer surgery at the Shiga University of Medical Science Hospital (Shiga, Japan) from 1997 to 2009; of these, most surgeries (n = 100) were performed 2008 onwards. The patient characteristics and surgery-related factors between two groups (with and without infection) were compared by the paired t-test and χ(2) test, including preoperative ADN levels, postoperative day 1 ADN levels, and ADN ratio (postoperative ADN levels/preoperative ADN levels) as baseline factors. Logistic regression analysis was performed to access the independent association between ADN ratio and postoperative infection. Finally, receiver operating curves (ROCs) were constructed to examine its clinical utility. RESULTS: Sixty patients (40%) experienced postoperative infections. The baseline values of age, American Society of Anesthesiologists physical status, total operating time, blood loss, surgical procedure, C-reactive protein (CRP) levels, preoperative ADN levels, and ADN ratio were significantly different between groups. Logistic regression analysis using these factors indicated that type 2 diabetes mellitus (T2DM) and ADN ratio were significantly independent variables (*p
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- 2013
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11. Biological and biochemical characterization of mice expressing prion protein devoid of the octapeptide repeat region after infection with prions.
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Yoshitaka Yamaguchi, Hironori Miyata, Keiji Uchiyama, Akira Ootsuyama, Sachiko Inubushi, Tsuyoshi Mori, Naomi Muramatsu, Shigeru Katamine, and Suehiro Sakaguchi
- Subjects
Medicine ,Science - Abstract
Accumulating lines of evidence indicate that the N-terminal domain of prion protein (PrP) is involved in prion susceptibility in mice. In this study, to investigate the role of the octapeptide repeat (OR) region alone in the N-terminal domain for the susceptibility and pathogenesis of prion disease, we intracerebrally inoculated RML scrapie prions into tg(PrPΔOR)/Prnp(0/0) mice, which express mouse PrP missing only the OR region on the PrP-null background. Incubation times of these mice were not extended. Protease-resistant PrPΔOR, or PrP(Sc)ΔOR, was easily detectable but lower in the brains of these mice, compared to that in control wild-type mice. Consistently, prion titers were slightly lower and astrogliosis was milder in their brains. However, in their spinal cords, PrP(Sc)ΔOR and prion titers were abundant and astrogliosis was as strong as in control wild-type mice. These results indicate that the role of the OR region in prion susceptibility and pathogenesis of the disease is limited. We also found that the PrP(Sc)ΔOR, including the pre-OR residues 23-50, was unusually protease-resistant, indicating that deletion of the OR region could cause structural changes to the pre-OR region upon prion infection, leading to formation of a protease-resistant structure for the pre-OR region.
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- 2012
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12. 6-DOF computation and marker design for magnetic 3D dexterous motion-tracking system.
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Jiawei Huang 0005, Tsuyoshi Mori, Kazuki Takashima, Shuichiro Hashi, and Yoshifumi Kitamura
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- 2016
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13. Supplementary Table 1, Figure 1 from Epigenetic Inactivation of the Potential Tumor Suppressor Gene FOXF1 in Breast Cancer
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Saraswati Sukumar, Tej K. Pandita, Pedram Argani, Helen Sadik, Mary Jo Fackler, Tsuyoshi Mori, Liangfeng Han, Xiaohui Liang, Ji Shin Lee, and Pang-Kuo Lo
- Abstract
Supplementary Table 1, Figure 1 from Epigenetic Inactivation of the Potential Tumor Suppressor Gene FOXF1 in Breast Cancer
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- 2023
14. Data from Epigenetic Inactivation of the Potential Tumor Suppressor Gene FOXF1 in Breast Cancer
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Saraswati Sukumar, Tej K. Pandita, Pedram Argani, Helen Sadik, Mary Jo Fackler, Tsuyoshi Mori, Liangfeng Han, Xiaohui Liang, Ji Shin Lee, and Pang-Kuo Lo
- Abstract
The expression of several members of the FOX gene family is known to be altered in a variety of cancers. We show that in breast cancer, FOXF1 gene is a target of epigenetic inactivation and that its gene product exhibits tumor-suppressive properties. Loss or downregulation of FOXF1 expression is associated with FOXF1 promoter hypermethylation in breast cancer cell lines and in invasive ductal carcinomas. Methylation of FOXF1 in invasive ductal carcinoma (37.6% of 117 cases) correlated with high tumor grade. Pharmacologic unmasking of epigenetic silencing in breast cancer cells restored FOXF1 expression. Re-expression of FOXF1 in breast cancer cells with epigenetically silenced FOXF1 genes led to G1 arrest concurrent with or without apoptosis to suppress both in vitro cell growth and in vivo tumor formation. FOXF1-induced G1 arrest resulted from a blockage at G1-S transition of the cell cycle through inhibition of the CDK2-RB-E2F cascade. Small interfering RNA–mediated depletion of FOXF1 in breast cancer cells led to increased DNA re-replication, suggesting that FOXF1 is required for maintaining the stringency of DNA replication and genomic stability. Furthermore, expression profiling of cell cycle regulatory genes showed that abrogation of FOXF1 function resulted in increased expression of E2F-induced genes involved in promoting the progression of S and G2 phases. Therefore, our studies have identified FOXF1 as a potential tumor suppressor gene that is epigenetically silenced in breast cancer, which plays an essential role in regulating cell cycle progression to maintain genomic stability. Cancer Res; 70(14); 6047–58. ©2010 AACR.
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- 2023
15. Supplementary Figure 2 from Epigenetic Inactivation of the Potential Tumor Suppressor Gene FOXF1 in Breast Cancer
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Saraswati Sukumar, Tej K. Pandita, Pedram Argani, Helen Sadik, Mary Jo Fackler, Tsuyoshi Mori, Liangfeng Han, Xiaohui Liang, Ji Shin Lee, and Pang-Kuo Lo
- Abstract
Supplementary Figure 2 from Epigenetic Inactivation of the Potential Tumor Suppressor Gene FOXF1 in Breast Cancer
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- 2023
16. Supplementary Methods from Epigenetic Inactivation of the Potential Tumor Suppressor Gene FOXF1 in Breast Cancer
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Saraswati Sukumar, Tej K. Pandita, Pedram Argani, Helen Sadik, Mary Jo Fackler, Tsuyoshi Mori, Liangfeng Han, Xiaohui Liang, Ji Shin Lee, and Pang-Kuo Lo
- Abstract
Supplementary Methods from Epigenetic Inactivation of the Potential Tumor Suppressor Gene FOXF1 in Breast Cancer
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- 2023
17. Supplementary Table 2 from Epigenetic Inactivation of the Potential Tumor Suppressor Gene FOXF1 in Breast Cancer
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Saraswati Sukumar, Tej K. Pandita, Pedram Argani, Helen Sadik, Mary Jo Fackler, Tsuyoshi Mori, Liangfeng Han, Xiaohui Liang, Ji Shin Lee, and Pang-Kuo Lo
- Abstract
Supplementary Table 2 from Epigenetic Inactivation of the Potential Tumor Suppressor Gene FOXF1 in Breast Cancer
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- 2023
18. IM6D: magnetic tracking system with 6-DOF passive markers for dexterous 3D interaction and motion.
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Jiawei Huang 0005, Tsuyoshi Mori, Kazuki Takashima, Shuichiro Hashi, and Yoshifumi Kitamura
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- 2015
- Full Text
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19. Spontaneous generation of distinct prion variants with recombinant prion protein from a baculovirus-insect cell expression system
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Morikazu Imamura, Naoko Tabeta, Yoshifumi Iwamaru, Hanae Takatsuki, Tsuyoshi Mori, and Ryuichiro Atarashi
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Mice ,Insecta ,Prions ,Biophysics ,Animals ,Cell Biology ,Molecular Biology ,Biochemistry ,Baculoviridae ,Prion Proteins ,Recombinant Proteins ,Prion Diseases - Abstract
Prion diseases are transmissible and progressive neurodegenerative disorders characterized by abnormal prion protein (PrP
- Published
- 2022
20. Pentosan Polysulfate Induces Latent Prion Infection In Fukuoka-1 Strain Infected Cell Culture
- Author
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Hanae Takatsuki, Morikazu Imamura, Tsuyoshi Mori, and Ryuichiro Atarashi
- Abstract
Each prion strain has its own characteristics and the efficacy of anti-prion drugs varies. Screening of prion disease therapeutics is typically evaluated by measuring amounts of protease-resistant prion protein (PrP-res). However, it remains unclear whether such measurements correlate with seeding activity, which can be evaluated by real-time quaking-induced conversion (RT-QuIC). In this study, the effects of anti-prion compounds pentosan polysulfate (PPS), Congo red, and alprenolol were measured in N2a58 cells infected with Fukuoka-1 (FK1) or 22L strain. The compounds abolished PrP-res and seeding activity, except for N2a58/FK1 treated with PPS. Interestingly, the seeding activity of N2a58/FK1 was much lower in the presence of PPS, but that was maintained thereafter; indeed, when PPS was removed, seeding activity and PrP-res gradually recovered to their original levels. These results indicate that prion latent infection is induced by PPS in a strain-dependent manner. Furthermore, for protein misfolding cyclic amplification (PMCA), the anti-prion effect of PPS decreased in FK1 compared to 22L, suggesting that the difference in effect observed between these two occurs at the level of the direct conversion. Our findings demonstrate that the advantages of RT-QuIC and PMCA can be exploited for more accurate assessment of therapeutic drug screening, reflecting strain differences.
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- 2022
21. Discrimination between L-type and C-type bovine spongiform encephalopathy by the strain-specific reactions of real-time quaking-induced conversion
- Author
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Kaori Ubagai, Hanae Takatsuki, Tsuyoshi Mori, Shigeo Fukuda, Yuzuru Taguchi, Ryuichiro Atarashi, Noriyuki Nishida, and Soichi Kageyama
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0301 basic medicine ,animal diseases ,Bovine spongiform encephalopathy ,Biophysics ,Hamster ,Biochemistry ,Prion Proteins ,law.invention ,Diagnosis, Differential ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Species Specificity ,Computer Systems ,law ,Cricetinae ,Escherichia ,mental disorders ,medicine ,Animals ,Prion protein ,Molecular Biology ,biology ,Strain (chemistry) ,Chemistry ,Brain ,food and beverages ,Cell Biology ,Amyloid fibril ,biology.organism_classification ,medicine.disease ,Molecular biology ,nervous system diseases ,Encephalopathy, Bovine Spongiform ,030104 developmental biology ,030220 oncology & carcinogenesis ,Recombinant DNA ,Cattle - Abstract
Real-time quaking-induced conversion (RT-QUIC) assays using Escherichia coli-derived purified recombinant prion protein (rPrP) enable us to amplify a trace amount of the abnormal form of PrP (PrPSc) from specimens. This technique can be useful for the early diagnosis of both human and animal prion diseases and the assessment of prion contamination. In the present study, we demonstrated that there are strain-specific differences in the RT-QUIC reactions between an atypical form of bovine spongiform encephalopathy (BSE), l-BSE, and classical BSE (C-BSE). Whereas mouse rPrP (rMoPrP) was efficiently converted to amyloid fibrils in the presence of PrPSc seed derived from either l-BSE or C-BSE, hamster rPrP (rHaPrP) was converted only in l-BSE, not C-BSE. These characteristics were preserved in the second round reaction, but gradually weakened in the subsequent rounds and were completely lost by the fifth round, most likely due to the selective growth advantage of nonspecific rPrP amyloid fibrils in the RT-QUIC. Our findings further enhance the discrimination of prion strains using RT-QUIC, and further our understanding of the molecular basis of prion strains.
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- 2020
22. [Primary Pulmonary Amyloidosis with a Localized Consolidation:Report of Two Cases]
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Yoshihiko, Goto, Miyuu, Imai, Hidekazu, Tanaka, Sachiyo, Kosai, Takuro, Sakagami, Tsuyoshi, Mori, and Kazuhiro, Iyonaga
- Subjects
Aged, 80 and over ,Lung Diseases ,Male ,Lung Neoplasms ,Positron Emission Tomography Computed Tomography ,Humans ,Female ,Immunoglobulin Light-chain Amyloidosis ,Amyloidosis ,Lung ,Aged - Abstract
We experienced two cases of primary pulmonary amyloidosis with a localized consolidation. Case 1 is a 80-year-old man, who was found to have an abnormal chest nodular shadow with blurred margin at a medical examination. Chest computed tomography( CT) showed a localized consolidation on the periphery of the upper lobe of the right lung. A CT-guided biopsy was performed. Case 2 is a 66-year-old woman, who was found to have an abnormal chest opacity at a medical examination. Chest CT showed a localized gathering of small nodules in the right lower lobe. Gradual enlargement was noted by follow up CT and the accumulation of fluorodeoxyglucose (FDG) was shown by PET/CT. In consideration of primary lung cancer or malignant lymphoma, right lower lobectomy was performed. Both cases were pathologically diagnosed as pulmonary amyloidosis. Since no findings of amyloid deposits in other organs or of existence of any blood disorders, a diagnosis of primary pulmonary amyloidosis was made.
- Published
- 2021
23. Small renal cell carcinoma accompanied by extensive inferior vena cava tumor thrombus diagnosed by percutaneous transvenous biopsy
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Hikaru Tsukada, Nozomi Hayakawa, Koichiro Aida, Shinji Wada, Tsuyoshi Morimoto, Masatomo Doi, Hidefumi Mimura, Junki Koike, and Eiji Kikuchi
- Subjects
differential diagnosis ,inferior vena cava ,small renal cell carcinoma ,transvenous biopsy ,tumor thrombus ,Diseases of the genitourinary system. Urology ,RC870-923 - Abstract
Introduction Up to 10% of patients with renal cell carcinoma present with tumor thrombus in the inferior vena cava. We report that a case of small renal cell carcinoma with tumor thrombus extending above the diaphragm for which transvenous biopsy was performed for diagnosis. Case presentation A 79‐year‐old man performed computed tomography to evaluate hepatic dysfunction, which revealed intravenous tumor extending above the diaphragm and a 15‐mm‐sized exophytic tumor in right kidney. Imaging suggested that the renal tumor was renal cell carcinoma. As this tumor was small and exophytic, confirmation of the intravenous tumor being tumor thrombus associated with renal cell carcinoma was difficult. We simultaneously performed transvenous biopsy on the intravenous tumor and percutaneous biopsy on the renal tumor for obtaining histologic diagnoses. The final diagnosis was small renal cell carcinoma accompanied by tumor thrombus above the diaphragm. Conclusion Transvenous biopsy may be useful for the definitive diagnosis of inferior vena cava‐tumor thrombus in cases of small renal cell carcinoma.
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- 2024
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24. Elucidation of the Molecular Basis of Abnormal Prion Protein (PrP) Formation in a Cell-Free System Using Baculovirus and Insect Cell-derived Recombinant PrP
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Hanae Takatsuki, Yoshifumi Iwamaru, Tsuyoshi Mori, Ryuichiro Atarashi, and Morikazu Imamura
- Subjects
Insecta ,animal diseases ,Pharmaceutical Science ,In Vitro Techniques ,Prion Proteins ,law.invention ,Cell-free system ,Glycosaminoglycan ,chemistry.chemical_compound ,In vivo ,law ,Nucleic Acids ,medicine ,Animals ,Glycosaminoglycans ,Pharmacology ,Cell-Free System ,Heparin ,Heparan sulfate ,Recombinant Proteins ,In vitro ,nervous system diseases ,Cell biology ,chemistry ,Nucleic acid ,Recombinant DNA ,Heparitin Sulfate ,Baculoviridae ,medicine.drug - Abstract
The molecular basis underlying the conversion of normal prion protein (PrPC) into abnormal prion protein (PrPSc) has not been fully elucidated. The protein-misfolding cyclic amplification (PMCA) technique, which can amplify PrPSc in vitro with the use of intermittent sonication, mimics the process of in vivo PrPSc replication. Accumulating evidence suggests that co-factors other than PrP may play a crucial role in the faithful replication of PrPSc. In conventional PMCA, brain homogenates (BHs) from normal animals are used as the PrPC substrate. Since BHs contain many impurities, it is difficult to identify the co-factors using conventional PMCA. Thus, we developed a modified PMCA system using baculovirus and insect cell-derived recombinant PrP as a substrate (insect cell PMCA; iPMCA). We demonstrated that nucleic acids and glycosaminoglycans (GAGs) such as heparan sulfate (HS) or its analogue heparin (HP) are critical for PrPSc amplification in iPMCA. Of note, the addition of HS or HP restored the conversion efficiency in iPMCA under nucleic acid-depleted conditions. Moreover, the iPMCA products were infectious and preserved the strain properties of the input seed PrPSc. These data suggest that not only nucleic acids but also some GAGs play an important role in facilitating faithful replication of prions, at least in vitro.
- Published
- 2019
25. Infiltration of CD4, CD8, CD56, and Fox-P3-positive lymphocytes in breast carcinoma tissue after neoadjuvant chemotherapy with or without trastuzumab1
- Author
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Yuki Kawai, Satoshi Murata, Mitsuaki Ishida, Kaori Tomida, Masaji Tani, Ryoji Kushima, Naoko Itoi, Tsuyoshi Mori, Tomoko Umeda, and Tomoharu Shimizu
- Subjects
0301 basic medicine ,Antibody-dependent cell-mediated cytotoxicity ,Cancer Research ,Cellular immunity ,Stromal cell ,business.industry ,Tumor-infiltrating lymphocytes ,General Medicine ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Immune system ,Oncology ,Trastuzumab ,030220 oncology & carcinogenesis ,Cancer research ,Medicine ,Cytotoxic T cell ,business ,CD8 ,medicine.drug - Abstract
BACKGROUND: Trastuzumab (Tz) is assumed to prime antibody-dependent cellular cytotoxicity (ADCC); however, it remains unclear whether Tz therapy can clinically induce adaptive cellular immunity. OBJECTIVE: Adaptive Cellular Immune Effect of Tz Therapy. METHODS: This study included 29 surgical invasive breast carcinomas administered neoadjuvant chemotherapy with Tz (15 cases) or without Tz (14 cases). The numbers of immunoreactive cells (CD4, CD8, CD56, and Fox-P3) in three different compartments (intratumoral, adjacent stromal, and distant stromal) were determined. RESULTS: The average number of adjacent stromal CD4-positive, CD8-positive, and Fox-P3-positive cells in the Tz+ group was significantly greater than that in the Tz− group (p = 0.036, 0.0049, and 0.043, respectively). However, the number of Fox-P3-positive cells was much less than that of CD4-positive cells. Moreover, distant stromal CD4-positive and CD8-positive cells in the Tz+ group was also significantly greater than that of the Tz− group (p = 0.029 and 0.032, respectively). Only a small number of CD56-positive natural killer cells, playing a main role in ADCC, accumulated at the tumor site after Tz therapy. CONCLUSIONS: The results suggest that Tz therapy induces adaptive cellular immunity, including infiltration of both CD4-positive helper T cells and CD8-positive cytotoxic T cells into the breast carcinoma lesion.
- Published
- 2019
26. Type I interferon protects neurons from prions in in vivo models
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Takujiro Homma, Daisuke Ishibashi, Katsuya Satoh, Ryuichiro Atarashi, Noriyuki Nishida, Takayuki Fuse, Kazunori Sano, Takehiro Nakagaki, and Tsuyoshi Mori
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0301 basic medicine ,Prions ,animal diseases ,Clinical Neurology ,Alpha interferon ,innate immune system ,Receptor, Interferon alpha-beta ,Biology ,Prion Proteins ,Prion Diseases ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Immune system ,Interferon ,medicine ,Animals ,Humans ,type I interferon (I-IFN) ,Neurons ,Innate immune system ,Brain ,Original Articles ,prion infection ,Virology ,Immunity, Innate ,nervous system diseases ,Mice, Inbred C57BL ,Editor's Choice ,030104 developmental biology ,Interferon Type I ,Interferon Regulatory Factor-3 ,Neurology (clinical) ,IRF3 ,030217 neurology & neurosurgery ,Ex vivo ,Interferon type I ,Signal Transduction ,Interferon regulatory factors ,medicine.drug - Abstract
Using cell culture and animal models of prion diseases, Ishibashi et al. show that type I interferon signalling interferes with prion infection in mammals. A selective type I interferon receptor agonist inhibits prion invasion and prolongs survival of prion-infected mice, suggesting potential clinical applications., Infectious prions comprising abnormal prion protein, which is produced by structural conversion of normal prion protein, are responsible for transmissible spongiform encephalopathies including Creutzfeldt-Jakob disease in humans. Prions are infectious agents that do not possess a genome and the pathogenic protein was not thought to evoke any immune response. Although we previously reported that interferon regulatory factor 3 (IRF3) was likely to be involved in the pathogenesis of prion diseases, suggesting the protective role of host innate immune responses mediated by IRF3 signalling, this remained to be clarified. Here, we investigated the reciprocal interactions of type I interferon evoked by IRF3 activation and prion infection and found that infecting prions cause the suppression of endogenous interferon expression. Conversely, treatment with recombinant interferons in an ex vivo model was able to inhibit prion infection. In addition, cells and mice deficient in type I interferon receptor (subunit interferon alpha/beta receptor 1), exhibited higher susceptibility to 22L-prion infection. Moreover, in in vivo and ex vivo prion-infected models, treatment with RO8191, a selective type I interferon receptor agonist, inhibited prion invasion and prolonged the survival period of infected mice. Taken together, these data indicated that the interferon signalling interferes with prion propagation and some interferon-stimulated genes might play protective roles in the brain. These findings may allow for the development of new strategies to combat fatal diseases.
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- 2019
27. Progression Potential of Ductal Carcinoma in situ Assessed by Genomic Copy Number Profiling
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Ken-ichi Mukaisho, Tomoko Umeda, Hiroyuki Sugihara, Takahisa Nakayama, Ryoji Kushima, Tsuyoshi Mori, Masaji Tani, Suzuko Moritani, and Mina Kitamura
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Adult ,In situ ,DNA Copy Number Variations ,Breast Neoplasms ,GATA3 Transcription Factor ,Lymph node metastasis ,Biology ,Pathology and Forensic Medicine ,03 medical and health sciences ,Breast cancer ,0302 clinical medicine ,Biomarkers, Tumor ,medicine ,Humans ,030212 general & internal medicine ,skin and connective tissue diseases ,neoplasms ,Molecular Biology ,Aged ,Aged, 80 and over ,Comparative Genomic Hybridization ,Paraffin Embedding ,Array-based comparative genomic hybridization ,Ductal carcinoma in situ ,GATA3 ,Unsupervised hierarchical clustering ,Cell Biology ,General Medicine ,Middle Aged ,Ductal carcinoma ,medicine.disease ,Subtyping ,body regions ,Carcinoma, Intraductal, Noninfiltrating ,030220 oncology & carcinogenesis ,Copy number alterations ,Disease Progression ,Cancer research ,Immunohistochemistry ,Female ,Tumor Suppressor Protein p53 ,Comparative genomic hybridization - Abstract
BACKGROUND:Ductal carcinoma in situ (DCIS) of the breast is heterogeneous in terms of the risk of progression to invasive ductal carcinoma (IDC). To treat DCIS appropriately for its progression risk, we classified individual DCIS by its profile of genomic changes into 2 groups and correlated them with clinicopathological progression factors., METHODS:We used surgically resected, formalin-fixed, paraffin-embedded tissues of 22 DCIS and 30 IDC lesions. We performed immunohistochemical intrinsic subtyping, array-based comparative genomic hybridization, and unsupervised clustering., RESULTS:The samples were divided into 2 major clusters, A and B. Cluster A showed a greater number of gene and chromosome copy number alterations, a larger IDC/DCIS ratio, a higher frequency of nonluminal subtype, a lower frequency of luminal subtype, and a higher nuclear grade, when compared with cluster B. However, there was no difference in the frequencies of lymph node metastasis between clusters A and B. We identified 9 breast-cancer-related genes, including TP53 and GATA3, that highly contributed to the discrimination of A and B clusters., CONCLUSION:Classification of breast tumors into rapidly progressive cluster A and the other (cluster B) may contributeto select the treatment appropriate for their progression risk.
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- 2018
28. Infiltration of CD4, CD8, CD56, and Fox-P3-positive lymphocytes in breast carcinoma tissue after neoadjuvant chemotherapy with or without trastuzumab
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Naoko Itoi, Tomoko Umeda, Mitsuaki Ishida, Satoshi Murata, Tsuyoshi Mori, Yuki Kawai, Kaori Tomida, Tomoharu Shimizu, Ryoji Kushima, and Masaji Tani
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0301 basic medicine ,Adult ,CD4-Positive T-Lymphocytes ,Cancer Research ,Immunity, Cellular ,Breast Neoplasms ,General Medicine ,Adaptive Immunity ,CD8-Positive T-Lymphocytes ,Middle Aged ,Trastuzumab ,CD56 Antigen ,Neoadjuvant Therapy ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Antineoplastic Agents, Immunological ,Oncology ,Cell Movement ,030220 oncology & carcinogenesis ,Antineoplastic Combined Chemotherapy Protocols ,Humans ,Female ,Hepatocyte Nuclear Factor 3-gamma ,Aged - Abstract
Trastuzumab (Tz) is assumed to prime antibody-dependent cellular cytotoxicity (ADCC); however, it remains unclear whether Tz therapy can clinically induce adaptive cellular immunity.Adaptive Cellular Immune Effect of Tz Therapy.This study included 29 surgical invasive breast carcinomas administered neoadjuvant chemotherapy with Tz (15 cases) or without Tz (14 cases). The numbers of immunoreactive cells (CD4, CD8, CD56, and Fox-P3) in three different compartments (intratumoral, adjacent stromal, and distant stromal) were determined.The average number of adjacent stromal CD4-positive, CD8-positive, and Fox-P3-positive cells in the Tz+ group was significantly greater than that in the Tz- group (p = 0.036, 0.0049, and 0.043, respectively). However, the number of Fox-P3-positive cells was much less than that of CD4-positive cells. Moreover, distant stromal CD4-positive and CD8-positive cells in the Tz+ group was also significantly greater than that of the Tz- group (p = 0.029 and 0.032, respectively). Only a small number of CD56-positive natural killer cells, playing a main role in ADCC, accumulated at the tumor site after Tz therapy.The results suggest that Tz therapy induces adaptive cellular immunity, including infiltration of both CD4-positive helper T cells and CD8-positive cytotoxic T cells into the breast carcinoma lesion.
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- 2019
29. A Case of Myxofibrosarcoma in the Abdominal Wall
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Nobuhito Nitta, Sachiko Kaida, Tomoharu Shimizu, Tsuyoshi Yamaguchi, Masaji Tani, and Tsuyoshi Mori
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Abdominal wall ,medicine.medical_specialty ,medicine.anatomical_structure ,business.industry ,medicine ,Myxofibrosarcoma ,Radiology ,business - Published
- 2018
30. Ethanolamine Is a New Anti-Prion Compound
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Agriani Dini Pasiana, Hideyuki Hara, Hanae Takatsuki, Suehiro Sakaguchi, Keiji Uchiyama, Ryuichiro Atarashi, Junji Chida, Morikazu Imamura, and Tsuyoshi Mori
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Gene isoform ,ethanolamine ,PrPSc Proteins ,QH301-705.5 ,animal diseases ,Scrapie ,Article ,Catalysis ,Prion Diseases ,Inorganic Chemistry ,Mice ,chemistry.chemical_compound ,Ethanolamine ,Oral administration ,Cell Line, Tumor ,medicine ,Animals ,Physical and Theoretical Chemistry ,Prion protein ,Biology (General) ,prions ,protein misfolding ,Molecular Biology ,QD1-999 ,Spectroscopy ,Mice, Inbred ICR ,therapy ,Organic Chemistry ,Neurodegeneration ,neurodegeneration ,Brain ,General Medicine ,Mouse Neuroblastoma ,medicine.disease ,Molecular biology ,Computer Science Applications ,nervous system diseases ,Chemistry ,chemistry ,prion protein ,Protein folding ,sense organs - Abstract
Prion diseases are a group of fatal neurodegenerative disorders caused by accumulation of proteinaceous infectious particles, or prions, which mainly consist of the abnormally folded, amyloidogenic prion protein, designated PrPSc. PrPSc is produced through conformational conversion of the cellular isoform of prion protein, PrPC, in the brain. To date, no effective therapies for prion diseases have been developed. In this study, we incidentally noticed that mouse neuroblastoma N2a cells persistently infected with 22L scrapie prions, termed N2aC24L1-3 cells, reduced PrPSc levels when cultured in advanced Dulbecco’s modified eagle medium (DMEM) but not in classic DMEM. PrPC levels remained unchanged in prion-uninfected parent N2aC24 cells cultured in advanced DMEM. These results suggest that advanced DMEM may contain an anti-prion compound(s). We then successfully identified ethanolamine in advanced DMEM has an anti-prion activity. Ethanolamine reduced PrPSc levels in N2aC24L1-3 cells, but not PrPC levels in N2aC24 cells. Also, oral administration of ethanolamine through drinking water delayed prion disease in mice intracerebrally inoculated with RML scrapie prions. These results suggest that ethanolamine could be a new anti-prion compound.
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- 2021
31. External focus instruction using a paper balloon: impact on trunk and lower extremity muscle activity in isometric single-leg stance for healthy males
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Koji Murofushi, Tsuyoshi Morito, Hiroshi Akuzawa, Tomoki Oshikawa, Yu Okubo, Koji Kaneoka, Sho Mitomo, and Kazuyoshi Yagishita
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trunk muscle activation ,external focus instruction ,contralateral training ,fine-wire electrode analysis ,single-leg stance ,Sports ,GV557-1198.995 - Abstract
IntroductionCore stability is crucial for preventing and rehabilitating lumbar spine injuries. An external focus instruction using a paper balloon is an effective way to activate the trunk muscles. However, the degree of trunk and lower extremity muscle activation during single leg stance with external focus instruction using a paper balloon is unknown. This study aimed to investigate the core muscle involving activity in the trunk and lower extremities on both the support and non-support sides with or without using external focus instruction using a paper balloon during isometric single-leg stance.MethodsThirteen healthy males aged 20–28 years volunteered to take part in this study and performed a single leg stance task with and without an external focus instruction, pressing their non-supporting foot onto a paper balloon without crushing it. The participant's muscle electrical activity was recorded during the single leg task using surface EMG and intramuscular EMG for six trunk muscles (transversus abdominis, internal oblique, external oblique, rectus abdominis, multifidus, and lumbar erector spinae) and five lower extremity muscles (gluteus maximus, gluteus medius, adductor longus, rectus femoris, and biceps femoris)ResultsCompared to the normal single leg stance, the external focus instruction task using a paper balloon showed significantly increased transversus abdominis (p
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- 2024
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32. Significance of the p38MAPK-CRP2 axis in myofibroblastic phenotypic transition
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Ken’ichiro Hayashi, Reuben Jacob Labios, Tsuyoshi Morita, Atsushige Ashimori, Ren Aoki, Masanori Mikuni, and Kazuhiro Kimura
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crp2 ,p38mapk ,mrtf ,myofibroblasts ,retinal pigment epithelial cells ,Science ,Biology (General) ,QH301-705.5 - Abstract
We have recently demonstrated that a LIM domain protein, cysteine and glycine-rich protein 2 (CSRP2 [CRP2]), plays a vital role in the functional expression of myofibroblasts and cancer-associated fibroblasts. CRP2 binds directly to myocardin-related transcription factors (MRTF [MRTF-A or MRTF-B]) and serum response factor (SRF) to stabilize the MRTF/SRF/CArG-box complex, leading to the expression of smooth muscle cell (SMC) genes such as α-smooth muscle actin (α-SMA) and collagens. These are the marker genes for myofibroblasts. Here, we show that the adhesion of cultured human skin fibroblasts (HSFs) to collagen reduces the myofibroblastic features. HSF adhesion to collagen suppresses the expression of CRP2 and CSRP2-binding protein (CSRP2BP [CRP2BP]) and reduces the expression of SMC genes. Although CRP2BP is known as an epigenetic factor, we find that CRP2BP also acts as an adaptor protein to enhance the function of CRP2 mentioned above. This CRP2BP function does not depend on its histone acetyltransferase activity. We also addressed the molecular mechanism of the reduced myofibroblastic features of HSFs on collagen. HSF adhesion to collagen inhibits the p38MAPK-mediated pathway, and reducing the p38MAPK activity decreases the expression of CRP2 and SMC genes. Thus, the activation of p38MAPK is critical for the myofibroblastic features. We also show evidence that CRP2 plays a role in the myofibroblastic transition of retinal pigment epithelial cells (RPEs). Like HSFs, such phenotypic modulation of RPEs depends on the p38MAPK pathway. Key words: CRP2, p38MAPK, MRTF, myofibroblasts, retinal pigment epithelial cells
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- 2023
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33. Expression of secretory calcium-binding phosphoprotein (scpp) genes in medaka during the formation and replacement of pharyngeal teeth
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Tsuyoshi Morita, Shin Matsumoto, and Otto Baba
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Scpp ,Medaka ,Pharyngeal teeth ,In situ hybridization ,Dentistry ,RK1-715 - Abstract
Abstract Background Analyses of tooth families and tooth-forming units in medaka with regard to tooth replacement cycles and the localization of odontogenic stem cell niches in the pharyngeal dentition clearly indicate that continuous tooth replacement is maintained. The secretory calcium-binding phosphoprotein (scpp) gene cluster is involved in the formation of mineralized tissues, such as dental and bone tissues, and the genes encoding multiple SCPPs are conserved in fish, amphibians, reptiles, and mammals. In the present study, we examined the expression patterns of several scpp genes in the pharyngeal teeth of medaka to elucidate their roles during tooth formation and replacement. Methods Himedaka (Japanese medaka, Oryzias latipes) of both sexes (body length: 28 to 33 mm) were used in this study. Real-time quantitative reverse transcription-polymerase chain reaction (PCR) (qPCR) data were evaluated using one-way analysis of variance for multi-group comparisons, and the significance of differences was determined by Tukey’s comparison test. The expression of scpp genes was examined using in situ hybridization (ISH) with a digoxigenin-labeled, single-stranded antisense probe. Results qPCR results showed that several scpp genes were strongly expressed in pharyngeal tissues. ISH analysis revealed specific expression of scpp1, scpp5, and sparc in tooth germ, and scpp5 was continually expressed in the odontoblasts of teeth attached to pedicles, but not in the osteoblasts of pedicles. In addition, many scpp genes were expressed in inner dental epithelium (ide), but not in odontoblasts, and scpp2 consistently showed epithelial-specific expression in the functional teeth. Taken together, these data indicate that specific expression of scpp2 and scpp5 may play a critical role in pharyngeal tooth formation in medaka. Conclusion We characterized changes in the expression patterns of scpp genes in medaka during the formation and replacement of pharyngeal teeth.
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- 2023
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34. Age of donor of human mesenchymal stem cells affects structural and functional recovery after cell therapy following ischaemic stroke
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Nobutaka Horie, Takeshi Ishikawa, Takayuki Matsuo, Tsuyoshi Izumo, Susumu Yamaguchi, Tsuyoshi Mori, Katsuya Satoh, Noriyuki Nishida, Takeshi Hiu, Yuhtaka Fukuda, Shunsuke Ishizaka, Yoichi Morofuji, and Hajime Maeda
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Male ,0301 basic medicine ,Aging ,medicine.medical_specialty ,Mesenchymal Stem Cell Transplantation ,Brain Ischemia ,Rats, Sprague-Dawley ,Cell therapy ,Brain ischemia ,03 medical and health sciences ,0302 clinical medicine ,Cell Movement ,Neurotrophic factors ,Internal medicine ,medicine ,Animals ,Humans ,Progenitor cell ,business.industry ,Neurogenesis ,Mesenchymal stem cell ,Age Factors ,Mesenchymal Stem Cells ,Original Articles ,equipment and supplies ,medicine.disease ,Tissue Donors ,Rats ,Stroke ,Transplantation ,030104 developmental biology ,Endocrinology ,Neurology ,Heterografts ,Neurology (clinical) ,Stem cell ,Cardiology and Cardiovascular Medicine ,business ,030217 neurology & neurosurgery - Abstract
Cell transplantation therapy offers great potential to improve impairments after stroke. However, the importance of donor age on therapeutic efficacy is unclear. We investigated the regenerative capacity of transplanted cells focusing on donor age (young vs. old) for ischaemic stroke. The quantities of human mesenchymal stem cell (hMSC) secreted brain-derived neurotrophic factor in vitro and of monocyte chemotactic protein-1 at day 7 in vivo were both significantly higher for young hMSC compared with old hMSC. Male Sprague-Dawley rats subjected to transient middle cerebral artery occlusion that received young hMSC (trans-arterially at 24 h after stroke) showed better behavioural recovery with prevention of brain atrophy compared with rats that received old hMSC. Histological analysis of the peri-infarct cortex showed that rats treated with young hMSC had significantly fewer microglia and more vessels covered with pericytes. Interestingly, migration of neural stem/progenitor cells expressing Musashi-1 positively correlated with astrocyte process alignment, which was more pronounced for young hMSC. Aging of hMSC may be a critical factor that affects cell therapy outcomes, and transplantation of young hMSC appears to provide better functional recovery through anti-inflammatory effects, vessel maturation, and neurogenesis potentially by the dominance of trophic factor secretion.
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- 2017
35. Preliminary Experiments of Series Elastic Actuator Prototype utilizing Mechanical Resonance
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Tsuyoshi Mori, Jun Kobayashi, and Akihiro Yoshimi
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Physics ,0209 industrial biotechnology ,Series (mathematics) ,020208 electrical & electronic engineering ,Robot manipulator ,Mechanical engineering ,02 engineering and technology ,DC motor ,Torsion spring ,Computer Science::Robotics ,020901 industrial engineering & automation ,0202 electrical engineering, electronic engineering, information engineering ,Torque ,Mechanical resonance ,Actuator ,Encoder - Abstract
This study proposes large force generation by a robotic manipulator by means of providing series elastic actuators exploiting mechanical resonance to it instead of powerful actuators. To this end, the authors designed and assembled a prototype of series elastic actuator that resonates at around 1.0 Hz on the basis of its model and identified parameters, and carried out preliminary experiments with it and a load. The prototype actuator consists of a geared DC motor with an encoder and an elastic element. The elastic element is made of two torsion springs so that it can generate torque in both directions. It was confirmed in the preliminary experiments that the prototype actuator resonated at around 0.7 Hz. In addition, in order to prove efficacy of mechanical resonance for large force generation, output torque of the prototype actuator with the elastic element and without it was estimated using their identified physical parameters and dynamics, and compared them. The estimation results showed that the prototype actuator exploiting mechanical resonance generated 2.24 times larger torque than the one without the elastic element.
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- 2019
36. [Two Cases of Unresectable/Advanced Breast Cancer with Pathological Complete Response after Combination Chemotherapy with Pertuzumab. Trastuzumab, and Docetaxel]
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Kaori, Tomida, Yuki, Kawai, Tsuyoshi, Mori, Mina, Kitamura, Hisataka, Kato, Sachiko, Sakai, Tomoharu, Shimizu, Tomoko, Umeda, Masahiro, Tatsumi, Kana, Shimada, and Masaji, Tani
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Adult ,Receptor, ErbB-2 ,Antineoplastic Combined Chemotherapy Protocols ,Humans ,Breast Neoplasms ,Female ,Taxoids ,Docetaxel ,Trastuzumab ,Antibodies, Monoclonal, Humanized ,Aged - Abstract
Combination chemotherapy with pertuzumab, trastuzumab, and docetaxel is recommended as the first-line treatment for patients with HER2-positive unresectable or metastatic breast cancer. We report 2 cases of unresectable breast cancer for which pertuzumab, trastuzumab, and docetaxel therapy was effective. Case 1: A woman in her 40s was diagnosed with TxN3aM0, Stage ⅢC, HER2-positive, hormone receptor-positive advanced breast cancer. After administration of 6 courses of pertuzumab, trastuzumab, and docetaxel therapy, she underwent surgery(Bt+Ax[Ⅱ]). Histopathological examination revealed that chemotherapy effect was Grade 3. Case 2: A woman in her 60s was diagnosed with de novo Stage Ⅳ, HER2- positive, hormone receptor-negative breast cancer. She was administered 8 courses of pertuzumab, trastuzumab, and docetaxel therapy as the third-line treatment, because she initially refused treatment. Thereafter, she underwent surgery(Bt+Ax [Ⅰ]). In both cases, histopathological examination revealed complete response after chemotherapy. Thus, combination therapy of pertuzumab and trastuzumab may improve the prognosis in patients with HER2-positive breast cancer.
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- 2019
37. Role of CRP2-MRTF interaction in functions of myofibroblasts
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Ken’ichiro Hayashi, Shinri Horoiwa, Kotaro Mori, Hiroshi Miyata, Reuben Jacob Labios, Tsuyoshi Morita, Yuka Kobayashi, Chiemi Yamashiro, Fumiaki Higashijima, Takuya Yoshimoto, Kazuhiro Kimura, and Yoshiaki Nakagawa
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crp2 ,3d structure ,myocardin-related transcription factor ,myofibroblast ,cancer-associated fibroblasts ,Science ,Biology (General) ,QH301-705.5 - Abstract
Inflammatory response induces phenotypic modulation of fibroblasts into myofibroblasts. Although transforming growth factor-βs (TGF-βs) evoke such transition, the details of the mechanism are still unknown. Here, we report that a LIM domain protein, cysteine-and glycine-rich protein 2 (CSRP2 [CRP2]) plays a vital role in the functional expression profile in myofibroblasts and cancer-associated fibroblasts (CAFs). Knock-down of CRP2 severely inhibits the expression of smooth muscle cell (SMC) genes, cell motility, and CAF-mediated collective invasion of epidermoid carcinoma. We elucidate the following molecular bases: CRP2 directly binds to myocardin-related transcription factors (MRTF-A/B [MRTFs]) and serum response factor (SRF) and stabilizes the MRTF/SRF/CArG-box complex to activate SMC gene expression. Furthermore, a three-dimensional structural analysis of CRP2 identifies the amino acids required for the CRP2-MRTF-A interaction. Polar amino acids in the C-terminal half (serine-152, glutamate-154, serine-155, threonine-156, threonine-157, and threonine-159 in human CRP2) are responsible for direct binding to MRTF-A. On the other hand, hydrophobic amino acids outside the consensus sequence of the LIM domain (tryptophan-139, phenylalanine-144, leucine-153, and leucine-158 in human CRP2) play a role in stabilizing the unique structure of the LIM domain. Key words: CRP2, 3D structure, myocardin-related transcription factor, myofibroblast, cancer-associated fibroblasts
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- 2023
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38. Prion-Seeding Activity Is widely Distributed in Tissues of Sporadic Creutzfeldt-Jakob Disease Patients
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Ban Mihara, Katsuya Satoh, Takayuki Fuse, Takehiro Nakagaki, Shigeo Murayama, Noriyuki Nishida, Mari Yoshida, Masaki Takao, Tsuyoshi Mori, Yasushi Iwasaki, Hanae Takatsuki, and Ryuichiro Atarashi
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0301 basic medicine ,Pathology ,medicine.medical_specialty ,animal diseases ,Central nervous system ,lcsh:Medicine ,Autopsy ,Disease ,Biology ,SD50 ,Creutzfeldt-Jakob Syndrome ,Prion Proteins ,General Biochemistry, Genetics and Molecular Biology ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Prion-seeding activity ,medicine ,Animals ,Humans ,Aged ,Infectivity ,lcsh:R5-920 ,lcsh:R ,Brain ,Non-neural tissue ,General Medicine ,Sporadic Creutzfeldt-Jakob disease ,Middle Aged ,Immunohistochemistry ,Virology ,Creutzfeldt-Jakob disease ,nervous system diseases ,Disease Models, Animal ,030104 developmental biology ,medicine.anatomical_structure ,Organ Specificity ,Case-Control Studies ,Prion ,Female ,lcsh:Medicine (General) ,030217 neurology & neurosurgery ,Research Paper - Abstract
Human prion diseases are neurodegenerative disorders caused by abnormally folded prion proteins in the central nervous system. These proteins can be detected using the quaking-induced conversion assay. Compared with other bioassays, this assay is extremely sensitive and was used in the present study to determine prion distribution in sporadic Creutzfeldt-Jakob disease patients at autopsy. Although infectivity of the sporadic form is thought to be restricted within the central nervous system, results showed that prion-seeding activities reach 106/g from a 50% seeding dose in non-neuronal tissues, suggesting that prion-seeding activity exists in non-neural organs, and we suggested that non-neural tissues of 106/g SD50 did not exist the infectivity., Highlights • Prion-seeding activities reach 106/g from a 50% seeding dose in non-neuronal tissues. • Results suggest that prion-seeding activity exists in neural and non-neural organs. A major problem for the diagnosis and management of human prion diseases is the lack of rapid and high-sensitive assays to measure low prion levels. Recent studies have tried to measure prion concentrations in non-neuronal tissues, but prion levels were not sufficient. Therefore, we developed the RT-QuIC method to measure prion-seeding activity in the non-neuronal, human tissues. The SD50 levels in the spleen, kidney, lung, and liver were 5.0–6.5, with different SD50 levels in the individual cases.
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- 2016
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39. IM6D
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Shuichiro Hashi, Kazuki Takashima, Yoshifumi Kitamura, Jiawei Huang, and Tsuyoshi Mori
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3D interaction ,Computer science ,business.industry ,Wireless ,Augmented reality ,Virtual reality ,Tracking (particle physics) ,business ,Computer Graphics and Computer-Aided Design ,Motion capture ,Simulation ,Electromagnetic induction - Abstract
We propose IM6D, a novel real-time magnetic motion-tracking system using multiple identifiable, tiny, lightweight, wireless and occlusion-free markers. It provides reasonable accuracy and update rates and an appropriate working space for dexterous 3D interaction. Our system follows a novel electromagnetic induction principle to externally excite wireless LC coils and uses an externally located pickup coil array to track each of the LC coils with 5-DOF. We apply this principle to design a practical motion-tracking system using multiple markers with 6-DOF and to achieve reliable tracking with reasonable speed. We also solved the principle's inherent dead-angle problem. Based on this method, we simulated the configuration of parameters for designing a system with scalability for dexterous 3D motion. We implemented an actual system and applied a parallel computation structure to increase the tracking speed. We also built some examples to show how well our system works for actual situations.
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- 2015
40. [A Case of Small Intestinal GIST with Long-Term Survival after Tumor Resection for Repeated Peritoneal Recurrence]
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Yoshitaka, Terada, Hiromichi, Sonoda, Toru, Miyake, Tomoharu, Shimizu, Tomoyuki, Ueki, Katsushi, Takebayashi, Sachiko, Kaida, Tsuyoshi, Yamaguchi, Naomi, Kitamura, Hiroya, Iida, Hiroya, Akabori, Tsuyoshi, Mori, and Masaji, Tani
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Time Factors ,Gastrointestinal Stromal Tumors ,Recurrence ,Intestinal Neoplasms ,Intestine, Small ,Imatinib Mesylate ,Humans ,Antineoplastic Agents ,Female ,Combined Modality Therapy ,Peritoneal Neoplasms ,Aged - Abstract
A 70-year-old woman presenting with abdominal pain was admitted to our hospital. Abdominal contrast CT revealed a small intestine tumor of 10 cm with active bleeding and performed partial resection of the small intestine including tumor. Pathological findings were high risk GIST of the small intestine because of spindle cells and c-kit positive. Imatinib 400mg/day as adjuvant chemotherapy was administered. However administration was stopped for 15 days because of the Grade 4 erythema multiforme. Recurrence of peritoneal dissemination was observed in 2 years after surgery and tumor resection was performed, but complete resection was difficult. Within 5 years after surgery, tumor resection was performed on a total of 5 times peritoneal disseminative recurrences, and it was possible to avoid the appearance of symptoms due to tumor augmentation.
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- 2018
41. Nasogastric Tube Syndrome: A Severe Complication of Nasointestinal Ileus Tube
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Kenkichiro Taira, Satoshi Koyama, Tsuyoshi Morisaki, Takahiro Fukuhara, Ryouhei Donishi, and Kazunori Fujiwara
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upper airway obstruction ,nasogastric tube syndrome ,nasointestinal ileus tube ,laryngoscope ,vocal cord paralysis ,Diseases of the digestive system. Gastroenterology ,RC799-869 - Abstract
Nasogastric tube syndrome (NGTS) induced by a nasointestinal ileus tube is an uncommon but potentially life-threatening complication. NGTS often becomes serious and progresses to acute upper airway obstruction caused by bilateral vocal cord paralysis or laryngeal infection. Early detection and proper treatment of NGTS are necessary. We describe the case of a 78-year-old patient with this syndrome induced by a nasointestinal ileus tube. At administration, ileus was suspected based on physical examination and thoracoabdominal X-ray findings. A nasointestinal ileus tube was placed through the left nasal cavity. Three days after tube placement, hoarseness and wheezing were found during nutrition support team rounds. Upper airway obstruction was suspected and evaluated immediately with flexible laryngoscopy by an otolaryngologist. The nasointestinal ileus tube was removed. The symptoms decreased with prompt proper management. Immediate removal of the tube and early recognition of symptoms are the first steps in the treatment for this syndrome, in addition to the initiation of steroid, proton pump inhibitor, and antibiotic therapy. The cause of NGTS is thought to be continuous pressure on the hypopharynx and cervical esophagus. NGTS should be considered in patients with either nasogastric or nasointestinal ileus tubes. Early diagnosis and proper management of NGTS are important.
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- 2023
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42. Actin‐related protein 5 suppresses the cooperative activation of cardiac gene transcription by myocardin and MEF2
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Tsuyoshi Morita and Ken'ichiro Hayashi
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actin‐related protein ,ARP5 ,cardiomyopathy ,MEF2 ,myocardin ,MYOCD ,Biology (General) ,QH301-705.5 - Abstract
MYOCD is a transcription factor important for cardiac and smooth muscle development. We previously identified that actin‐related protein 5 (ARP5) binds to the N‐terminus of MYOCD. Here, we demonstrate that ARP5 inhibits the cooperative action of the cardiac‐specific isoform of MYOCD with MEF2. ARP5 overexpression in murine hearts induced cardiac hypertrophy and fibrosis, whereas ARP5 knockdown in P19CL6 cells significantly increased cardiac gene expression. ARP5 was found to bind to a MEF2‐binding motif of cardiac MYOCD and inhibit MEF2‐mediated transactivation by MYOCD. RNA‐seq analysis revealed 849 genes that are upregulated by MYOCD‐MEF2 and 650 genes that are repressed by ARP5. ARP5 expression increased with cardiomyopathy and was negatively correlated with the expression of Tnnt2 and Ttn, which were regulated by cardiac MYOCD‐MEF2. Overall, our data suggest that ARP5 is a potential suppressor of cardiac MYOCD during physiological and pathological processes.
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- 2023
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43. [Surgery for Multiple Gastrointestinal Stromal Tumors of the Small Intestine in Patients with Neurofibromatosis Type 1 - A Report of Three Cases]
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Reiko, Ohtake, Tsuyoshi, Yamaguchi, Satoshi, Murata, Sachiko, Kaida, Hiroshi, Yamamoto, Katsushi, Takebayashi, Hiroya, Akabori, Hiromichi, Sonoda, Tsuyoshi, Mori, Tomoharu, Shimizu, Shigeyuki, Naka, Shigeki, Banba, Tomoyuki, Tsujikawa, Akira, Ando, and Masaji, Tani
- Subjects
Male ,Neurofibromatosis 1 ,Treatment Outcome ,Gastrointestinal Stromal Tumors ,Humans ,Female ,Middle Aged ,Tomography, X-Ray Computed ,Aged ,Gastrointestinal Neoplasms - Abstract
We report 3 cases of multiple GIST of the small intestine in 3 patients with NF1 who have been followed for over 5 years. All patients presented with melena, and tumors of the small intestine suspected to be GIST were found on endoscopy. We performed partial resections of the small intestine for all 3 patients. After surgery, 1 patient had residual tumors that gradually enlarged during 8 year 2 months and another had residual tumors that have been stable for 8 years. In the third patient, we resected all the tumors, and there has been no sign of recurrence in 6 year 1 month.
- Published
- 2017
44. [Stage IV Gastric Cancer with Positive Peritoneal Washing Cytology or Peritoneal Dissemination Was Successfully Treated with Gastrectomy and Hyperthermic Intraperitoneal Chemotherapy(HIPEC)Followed by Systemic Chemotherapy - A Report of Two Cases]
- Author
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Tsuyoshi, Yamaguchi, Satoshi, Murata, Sachiko, Kaida, Reiko, Ohtake, Katsushi, Takebayashi, Hiroshi, Yamamoto, Toru, Miyake, Hiroya, Akabori, Hiromichi, Sonoda, Tsuyoshi, Mori, Tomoharu, Shimizu, Shigeyuki, Naka, Suzuko, Moritani, Ryoji, Kushima, and Masaji, Tani
- Subjects
Treatment Outcome ,Gastrectomy ,Stomach Neoplasms ,Antineoplastic Combined Chemotherapy Protocols ,Humans ,Female ,Hyperthermia, Induced ,Middle Aged ,Peritoneum ,Combined Modality Therapy ,Peritoneal Neoplasms ,Neoplasm Staging - Abstract
Survival of Stage IV gastric cancer is poor. We report 2 cases of Stage IV gastric cancer with positive peritoneal washing cytology or peritoneal dissemination that were successfully treated with gastrectomy and hyperthermic intraperitoneal chemotherapy( HIPEC)followed by systemic chemotherapy. Case 1: A 59-year-old woman. She was diagnosed with advanced gastric cancer and underwent gastrectomy with HIPEC. Her peritoneal washing cytology was positive during the gastrectomy. After the surgery, she underwent chemotherapy consisting of 8 courses of combination S-1 plus CPT-11 and 19 courses of PTX. It has been 5 years and 7 months since she had the surgery and she survives without recurrence of the cancer. Case 2: A 60-year-old woman. She was diagnosed with advanced gastric cancer and peritoneal dissemination(peritoneal cancer index: 3 points). She underwent gastrectomy, hemi-colectomy, and HIPEC. After the surgery, she underwent chemotherapy, 35 courses of combination S-1 plus PSK/DOC, and 13 courses of S-1 plus PSK. It has been 5 years since her surgery and she survives without exacerbation of the cancer. These cases suggest a gastrectomy and HIPEC followed by systemic chemotherapy may represent an effective treatment for advanced gastric cancer with a small amount of peritoneal metastasis.
- Published
- 2017
45. [Salvage Surgery after CRT for Advanced Esophageal Cancer Resulting in a Pathological Complete Response and More Than Five Years' Survival]
- Author
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Sachiko, Kaida, Satoshi, Murata, Tsuyoshi, Yamaguchi, Reiko, Ohtake, Katsushi, Takebayashi, Hiroshi, Yamamoto, Tomoyuki, Ueki, Toru, Miyake, Hiroya, Iida, Hiroya, Akabori, Hiromichi, Sonoda, Tsuyoshi, Mori, Tomoharu, Shimizu, Shigeyuki, Naka, and Masaji, Tani
- Subjects
Salvage Therapy ,Time Factors ,Esophageal Neoplasms ,Antineoplastic Combined Chemotherapy Protocols ,Humans ,Female ,Chemoradiotherapy ,Fluorouracil ,Cisplatin ,Middle Aged - Abstract
A 62-year-old woman visited our hospital because of dysphagia. She was diagnosed with upper-middle esophageal type 4 cancer, which was 9 cm in length, according to the results of endoscopy. Squamous cell carcinoma was demonstrated using endoscopic biopsy. A CT scan revealed that the tumor had directly invaded into the trachea(cT4). Chemoradiotherapy(CRT) (5-FU and CDDP with 50 Gy of radiation)was administered. Although CT after CRT resulted in shrinkage of the tumor and no further tracheal invasion, esophageal stenosis remained. Therefore, salvage surgery(subtotal esophagectomy with 3-field lymph node dissection)was performed. Pathologically, no carcinoma cells were found in the resected specimen and a com- plete response(grade 3)was diagnosed. The patient received adjuvant chemotherapy(tegafur/uracil at 300mg/day per os) for 1 year. The patient is alive with no relapse of carcinoma more than 5 years after the first treatment.
- Published
- 2017
46. [A Case of Breast Metastasis of Eccrine Porocarcinoma]
- Author
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Tsuyoshi, Mori, Mina, Kitamura, Kaori, Tomida, Yuki, Kawai, Sachiko, Sakai, Sachiko, Kaida, Toru, Miyake, Naomi, Kitamura, Hiroya, Akabori, Tsuyoshi, Yamaguchi, Hiromichi, Sonoda, Tomoharu, Shimizu, Shigeyuki, Naka, and Masaji, Tani
- Subjects
Aged, 80 and over ,Sweat Gland Neoplasms ,Treatment Outcome ,Lymphatic Metastasis ,Humans ,Breast Neoplasms ,Female ,Chemoradiotherapy ,Eccrine Porocarcinoma - Abstract
An 80's woman was diagnosed with eccrine porocarcinoma of the head in 2010.T he tumor was removed surgically but relapsed in the cervical and axillary lymph nodes 2 years later.The patient underwent surgery, and received systemic chemotherapy and radiation.Chest CT after treatment revealed an irregular mass and thickened skin in the left breast.Core needle biopsy specimens were used to diagnose metastasis of eccrine porocarcinoma.A wide excision with a 1 cm margin was performed under local anesthesia.After surgery, supraclavicular lymph node recurrence was detected.The patient received palliative care because there was no effective treatment available.Eccrine porocarcinoma is a rare malignant tumor of the intraepidermal sweat duct.Breast metastasis from malignant disease is also rare.To our knowledge, breast metastasis of eccrine porocarcinoma has not been reported.
- Published
- 2017
47. Solid plasticity and supercooled-liquid thermoplasticity of Zr–Cu-enriched hypoeutectic Zr–Cu–Ni–Al cast glassy alloys
- Author
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Hitto Tokunaga, Masahiko Nishijima, Kazutaka Fujita, Toshiyuki Shima, Yoshihiko Yokoyama, Tsuyoshi Mori, Masahiro Yamada, Tohru Yamasaki, and Takeshi Sato
- Subjects
Materials science ,Mechanical Engineering ,Metallurgy ,Alloy ,Viscometer ,engineering.material ,Condensed Matter Physics ,Nanocrystalline material ,Viscosity ,Mechanics of Materials ,engineering ,General Materials Science ,Composite material ,Supercooling ,Glass transition ,Eutectic system ,Tensile testing - Abstract
The plasticity of Zr–Cu enriched hypoeutectic Zr–Cu–Ni–Al cast glassy alloys (CGAs) was investigated to determine their tensile elongation as a solid at room temperature, and their precise thermoplastic moldability in a supercooled liquid state. The viscosity of the supercooled liquids was measured by using a penetration viscometer at a high-speed heating rate of 400 K/min. The results obtained show that with an increase in the Zr content, the glass transition temperature (Tg) tends to decrease, whereas the crystallization temperature (Tx) tends to increase. We observed tensile elongation with plural sliding shear bands in the Zr–Cu enriched Zr65Cu20Ni5Al10 CGAs at room temperature. Furthermore, the hypoeutectic Zr65Cu18Ni7Al10 CGA exhibits the widest ΔTx (=Tx−Tg) of about 170 K, with a low viscosity in the order of 105 Pa s being observed in the supercooled liquid under a heating rate of 400 K/min. The potential for printing micro-patterns on the surface of a glassy Zr65Cu18Ni7Al10 alloy is demonstrated by means of tough nanocrystalline Ni–W electro-plating molds.
- Published
- 2014
48. 6-DOF computation and marker design for magnetic 3D dexterous motion-tracking system
- Author
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Shuichiro Hashi, Yoshifumi Kitamura, Tsuyoshi Mori, Jiawei Huang, and Kazuki Takashima
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3D interaction ,Computer science ,business.industry ,Computation ,ComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISION ,020207 software engineering ,Input device ,02 engineering and technology ,Kinematics ,Translation (geometry) ,Motion capture ,Set (abstract data type) ,020303 mechanical engineering & transports ,0203 mechanical engineering ,0202 electrical engineering, electronic engineering, information engineering ,Computer vision ,Artificial intelligence ,business ,Rotation (mathematics) ,ComputingMethodologies_COMPUTERGRAPHICS - Abstract
We describe our approach that derives reliable 6-DOF information including the translation and the rotation of a rigid marker in a 3D space from a set of insufficient 5-DOF measurements. As a practical example, we carefully constructed a prototype and its design and evaluated it in our 3D dexterous motion-tracking system, IM6D, which is our novel real-time magnetic 3D motion-tracking system that uses multiple identifiable, tiny, lightweight, wireless, and occlusion-free markers. The system contains two key technologies; a 6-DOF computation algorithm and a marker design for 6D marker. The 6-DOF computation algorithm computes the result of complete 6-DOF information including translation and rotation in 3D space for a single rigid marker that consists of three LC coils. We propose several possible approaches for implementation, including geometric, matrix-based kinematics, and computational approaches. In addition, we introduce workflow to find an optimal marker design for the system to achieve the best compromise between its smallness and accuracy based on the tracking principle. We experimentally compare the performances of some typical marker prototypes with different layouts of LC coils. Finally, we also show another experimental result to prove the effectiveness of the results from the solutions in these two problems.
- Published
- 2016
49. The Kampo Medicine Goshajinkigan Prevents Neuropathy in Breast Cancer Patients Treated with Docetaxel
- Author
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Tohru Tani, Kaori Tomida, Yoshihiro Kubota, Tomoko Umeda, Tsuyoshi Mori, Yuki Kawai, and Hajime Abe
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Adult ,Cancer Research ,medicine.medical_specialty ,Epidemiology ,Visual analogue scale ,Breast Neoplasms ,Docetaxel ,Gastroenterology ,Breast cancer ,Oral administration ,Internal medicine ,Humans ,Medicine ,Aged ,business.industry ,Incidence (epidemiology) ,Public Health, Environmental and Occupational Health ,Common Terminology Criteria for Adverse Events ,Middle Aged ,medicine.disease ,Surgery ,Vitamin B 12 ,Regimen ,Oncology ,Concomitant ,Female ,Taxoids ,Medicine, Kampo ,Nervous System Diseases ,business ,Drugs, Chinese Herbal ,medicine.drug - Abstract
Background: Goshajinkigan (GJG) is used for the treatment of several neurological symptoms. We investigated the efficacy of GJG and mecobalamin (B12) against neurotoxicity associated with docetaxel (DOC) in breast cancer patients. Materials and Methods: Sixty breast cancer patients were treated with DOC. Thirty-three patients (GJG group) received oral administration of 7.5 g/day GJG and 27 patients (B12 group) received oral administration of 1500 μg/day B12. Neuropathy was evaluated according to DEB-NTC (Neurotoxicity Criteria of Debiopharm), Common Terminology Criteria for Adverse Events (NCI-CTC) ver. 3.0, and a visual analogue scale (VAS). This study employed a randomized open design. Results: The incidence of neuropathy was 39.3% in the GJG group, and 88.9% in the B12 group (p
- Published
- 2013
50. Feasibility Study of Docetaxel and Cyclophosphamide Six- Cycle Therapy as Adjuvant Chemotherapy for Japanese Human Epidermal Growth Factor Receptor 2-Negative Breast Cancer Patients
- Author
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Yoshihiro Kubota, Tsuyoshi Mori, Hajime Abe, Kaori Tomida, Tomoko Umeda, Tohru Tani, and Yuki Kawai
- Subjects
Adult ,Oncology ,endocrine system ,Cancer Research ,medicine.medical_specialty ,Cyclophosphamide ,Receptor, ErbB-2 ,Epidemiology ,Breast Neoplasms ,Docetaxel ,Breast cancer ,Asian People ,Internal medicine ,Antineoplastic Combined Chemotherapy Protocols ,medicine ,Humans ,Outpatient clinic ,Adverse effect ,Aged ,Epirubicin ,Neoplasm Staging ,business.industry ,Public Health, Environmental and Occupational Health ,Middle Aged ,Prognosis ,medicine.disease ,Regimen ,Chemotherapy, Adjuvant ,Feasibility Studies ,Female ,Taxoids ,Fluorouracil ,business ,Febrile neutropenia ,Follow-Up Studies ,medicine.drug - Abstract
Background: We compared treatment completion rates and safety of docetaxel and cyclophosphamide sixcycle therapy (TC6) with docetaxel followed by 5FU, epirubicin and cyclophosphamide (T-FEC) therapy in Japanese patients with human epidermal growth factor receptor 2 (HER2)-negative breast cancer. Materials and Methods: We administered TC6 q3w or T-FEC q3w to HER2-negative breast cancer patients. The primary endpoint of this trial was toxicity. As second endpoints, the treatment completion rate and relative dose intensity were evaluated. Results: The TC6 and T-FEC group consisted of 22 and 21 patients, respectively. Concerning hematological toxicity, grade 3 or higher adverse reactions included neutropenia and febrile neutropenia. As non-hematological adverse events, exanthema and peripheral neuropathy were frequently reported in the TC6 group, whereas more patients of the T-FEC group reported nausea and vomiting. In TC6, the treatment completion rate was 86.4% and the relative dose intensity of docetaxel was 93.2%. In T-FEC, the values were 95.2% and 98.9%, respectively. Conclusions: These results suggest that TC6 is tolerable in Japanese, and that this regimen can also be performed in outpatient clinics. However, with the TC6 regimen, the compliance was slightly lower than with the T-FEC regimen, and supportive therapy needs to be managed appropriately.
- Published
- 2013
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