Search

Your search keyword '"Tsumoto K"' showing total 772 results
Start Over Author "Tsumoto K"
772 results on '"Tsumoto K"'

1. MDM2 bound with a peptoid

Author

Yokomine, M., primary, Fukuda, Y., additional, Ago, H., additional, Matsuura, H., additional, Ueno, G., additional, Nagatoishi, S., additional, Yamamoto, M., additional, Tsumoto, K., additional, Jumpei, M., additional, and Sando, S., additional

Published
2024
Full Text
View/download PDF

5. Unbinding Transition Induced by Osmotic Pressure in Relation to Unilamellar Vesicle Formation

Author

Yamada, N. L., Hishida, M., Seto, H., Tsumoto, K., and Yoshimura, T.

Subjects
Condensed Matter - Soft Condensed Matter
Abstract

Small-angle X-ray scattering and phase-contrast microscopy experiments were performed to investigate the effect of the osmotic pressure on vesicle formation in a dioleoylphosphatidylcholine (DOPC)/water/NaI system. Multi-lamellar vesicles were formed when a pure lipid film was hydrated with an aqueous solution of NaI. On the other hand, uni-lamellar vesicles (ULVs) were formed when a lipid film mixed with an enough amount of NaI was hydrated. To confirm the effect of the osmotic pressure due to NaI, a free-energy calculation was performed. This result showed that the osmotic pressure induced an unbinding transition on the hydration process, which resulted in ULV formation.

Published
2006
Full Text
View/download PDF

13. B cell–intrinsic TBK1 is essential for germinal center formation during infection and vaccination in mice

Author

Lee, MSJ, Inoue, T, Ise, W, Matsuo-Dapaah, J, Wing, JB, Temizoz, B, Kobiyama, K, Hayashi, T, Patil, A, Sakaguchi, S, Simon, AK, Bezbradica, JS, Nagatoishi, S, Tsumoto, K, Inoue, J-I, Akira, S, Kurosaki, T, Ishii, KJ, and Coban, C

Subjects
B-Lymphocytes, Immunology, Receptors, Antigen, B-Cell, T-Lymphocytes, Helper-Inducer, Protein Serine-Threonine Kinases, Germinal Center, Infections, Immunity, Humoral, Mice, Gene Expression Regulation, Gene Knockdown Techniques, Host-Pathogen Interactions, Animals, Immunology and Allergy, Immunization, CD40 Antigens, Biomarkers, Signal Transduction
Abstract

The germinal center (GC) is a site where somatic hypermutation and clonal selection are coupled for antibody affinity maturation against infections. However, how GCs are formed and regulated is incompletely understood. Here, we identified an unexpected role of Tank-binding kinase-1 (TBK1) as a crucial B cell–intrinsic factor for GC formation. Using immunization and malaria infection models, we show that TBK1-deficient B cells failed to form GC despite normal Tfh cell differentiation, although some malaria-infected B cell–specific TBK1-deficient mice could survive by GC-independent mechanisms. Mechanistically, TBK1 phosphorylation elevates in B cells during GC differentiation and regulates the balance of IRF4/BCL6 expression by limiting CD40 and BCR activation through noncanonical NF-κB and AKTT308 signaling. In the absence of TBK1, CD40 and BCR signaling synergistically enhanced IRF4 expression in Pre-GC, leading to BCL6 suppression, and therefore failed to form GCs. As a result, memory B cells generated from TBK1-deficient B cells fail to confer sterile immunity upon reinfection, suggesting that TBK1 determines B cell fate to promote long-lasting humoral immunity.

Published
2021

Catalog

Books, media, physical & digital resources
See catalog results