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6. Primary Cilia are Required for Cell-Type Determination and Angiogenesis in Pituitary Development.

Author

Yoshida, Saishu, Tsuneoka, Yousuke, Tsukada, Takehiro, Nakakura, Takashi, Kawamura, Akira, Kai, Wataru, and Yoshida, Kiyotsugu

Subjects
CILIA & ciliary motion, NEOVASCULARIZATION, PITUITARY gland, CELL survival, CELL determination
Abstract

The functional maturation of the pituitary gland requires adequate cell differentiation and vascular network formation. Although spatiotemporal signaling and transcription factors are known to govern pituitary development, the involvement of primary cilia, nonmoving hair-like organelles, remains unclear. In this study, we uncovered the contribution of primary cilia to cell-type determination and vascular network formation during pituitary development. Homozygous knockout mice lacking a ciliary kinase, Dyrk2 −/−, exhibit abnormalities in ciliary structure and pituitary hypoplasia, accompanied by varying degrees of failure in differentiation among all types of hormone-producing cells in the anterior lobe. Aberrations in cell differentiation in Dyrk2 −/− mice arise from a decrease in the expression of crucial transcription factors, Lhx4 , Lhx3 , and Prop1 , resulting from the inactivity of Hedgehog (Hh) signaling during the early stages of development. Furthermore, the loss of Dyrk2 results in vascular system abnormalities during the middle to late stages of development. Mechanistically, transcriptome analyses revealed the downregulation of vitronectin-integrin αvβ3-VEGFR2 signaling, essential for orchestrating vascular development. Collectively, our findings demonstrate that primary cilia play a pivotal role as critical regulators of cell survival, cell determination, and angiogenesis during pituitary gland development through the activation of Hh signaling. These findings expand our understanding of the potential link between pituitary dysfunction in human disorders and ciliopathies. [ABSTRACT FROM AUTHOR]

Published
2024
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12. Contributors

Author

Amano, Masafumi, primary, Ando, Hironori, additional, Andoh, Tadashi, additional, Baker, Michael E., additional, Cameron, Melissa S., additional, Daubnerová, Ivana, additional, Donald, John A., additional, Fukumura, Keisuke, additional, Funaba, Masayuki, additional, Haraguchi, Shogo, additional, Hayakawa, Yoichi, additional, Hirako, Satoshi, additional, Hyodo, Susumu, additional, Iguchi, Taisen, additional, Inui, Akio, additional, Kaiya, Hiroyuki, additional, Kakizawa, Sho, additional, Kamiyama, Takumi, additional, Kanamori, Yohei, additional, Kanda, Shinji, additional, Katayama, Hidekazu, additional, Kato, Takashi, additional, Katsu, Yoshinao, additional, Katsuura, Goro, additional, Kawada, Tsuyoshi, additional, Kimura, Atsushi P., additional, Kitamura, Keiichiro, additional, Kobayashi, Yuki, additional, Kodani, Yu, additional, Konno, Norifumi, additional, Kuraku, Shigehiro, additional, Minakata, Hiroyuki, additional, Mita, Masatoshi, additional, Miyagawa, Shinichi, additional, Miyazato, Mikiya, additional, Mizusawa, Kanta, additional, Mori, Kenji, additional, Morishita, Fumihiro, additional, Moriyama, Shunsuke, additional, Nagasaki, Hiroshi, additional, Nagata, Shinji, additional, Nakamachi, Tomoya, additional, Niwa, Ryusuke, additional, Ogino, Yukiko, additional, Ogoshi, Maho, additional, Ohira, Tsuyoshi, additional, Ohki-Hamazaki, Hiroko, additional, Ohtaki, Hirokazu, additional, Ohyama, Yoshihiko, additional, Oka, Yoshitaka, additional, Okamoto, Naoki, additional, Onizawa, Moe, additional, Osugi, Tomohiro, additional, Saito, Yumiko, additional, Sakai, Takafumi, additional, Sakamoto, Hirotaka, additional, Sakamoto, Tatsuya, additional, Sakata, Ichiro, additional, Satake, Honoo, additional, Sato, Seiichi, additional, Sato, Tomomi, additional, Seike, Hitomi, additional, Sekiguchi, Toshio, additional, Shimizu, Munetaka, additional, Shinki, Toshimasa, additional, Shinoda, Tetsuro, additional, Shiomi, Kunihiro, additional, Suzuki, Nobuo, additional, Tachibana, Tetsuya, additional, Takahashi, Akiyoshi, additional, Takahashi, Toshio, additional, Takaoka, Akinori, additional, Takei, Yoshio, additional, Takenoya, Fumiko, additional, Takeuchi, Sakae, additional, Tanaka, Yoshiaki, additional, Toshinai, Koji, additional, Tsukada, Takehiro, additional, Tsutsui, Kazuyoshi, additional, Tsutsui, Naoaki, additional, Ubuka, Takayoshi, additional, Ukena, Kazuyoshi, additional, Wada, Nobuhiro, additional, Watanabe, Jun, additional, Wong, Marty Kwok-Shing, additional, Yamada, Taisho, additional, Yamaguchi, Yoko, additional, Yamanaka, Naoki, additional, Yamauchi, Kiyoshi, additional, Yuge, Shinya, additional, Zhou, Yijun, additional, and Žitňan, Dušan, additional

Published
2021
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22. Subcellular localization of Na+/K+-ATPase isoforms resolved by in situ hybridization chain reaction in the gill of chum salmon at freshwater and seawater.

Author

Wong, Marty Kwok Shing, Tsuneoka, Yousuke, and Tsukada, Takehiro

Abstract

The Na+/K+-ATPase (NKA) α1-isoforms were examined by in situ hybridization chain reaction (ISHCR) using short hairpin DNAs, and we showed triple staining of NKA α1a, α1b, and α1c transcripts in the gill of chum salmon acclimated to freshwater (FW) and seawater (SW). The NKA α1-isoforms have closely resembled nucleotide sequences, which could not be differentiated by conventional in situ hybridization. The ISHCR uses a split probe strategy to allow specific hybridization using regular oligo DNA, resulting in high specificity at low cost. The results showed that NKA α1c was expressed ubiquitously in gill tissue and no salinity effects were observed. FW lamellar ionocytes (type-I ionocytes) expressed cytoplasmic NKA α1a and nuclear NKA α1b transcripts. However, both transcripts of NKA α1a and α1b were present in the cytoplasm of immature type-I ionocytes. The developing type-I ionocytes increased the cytoplasmic volume and migrated to the distal region of the lamellae. SW filament ionocytes (type-II ionocytes) expressed cytoplasmic NKA α1b transcripts as the major isoform. Results from morphometric analysis and nonmetric multidimensional scaling indicated that a large portion of FW ionocytes was NKA α1b-rich, suggesting that isoform identity alone cannot mark the ionocyte types. Both immature or residual type-II ionocytes and type-I ionocytes were found on the FW and SW gills, suggesting that the chum salmon retains the potential to switch the ionocyte population to fit the ion-transporting demands, which contributes to their salinity tolerance and osmoregulatory plasticity. [ABSTRACT FROM AUTHOR]

Published
2023
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31. List of Contributors

Author

Amano, Masafumi, primary, Ando, Hironori, additional, Andoh, Tadashi, additional, Daubnerova, Ivana, additional, Donald, John A., additional, Forgan, Leonard G., additional, Haraguchi, Shogo, additional, Hayakawa, Yoichi, additional, Hirako, Satoshi, additional, Hyodo, Susumu, additional, Iguchi, Taisen, additional, Inui, Akio, additional, Kageyama, Haruaki, additional, Kaiya, Hiroyuki, additional, Kakizawa, Sho, additional, Kanda, Shinji, additional, Kaneko, Hiroyuki, additional, Kataoka, Hiroshi, additional, Katayama, Hidekazu, additional, Kato, Takashi, additional, Katsu, Yoshinao, additional, Katsuura, Goro, additional, Kawada, Tsuyoshi, additional, Kimura, Atsushi P., additional, Kobayashi, Yuki, additional, Konno, Norifumi, additional, Konogami, Tadafumi, additional, Minakata, Hiroyuki, additional, Mita, Masatoshi, additional, Miyagawa, Shinichi, additional, Miyazato, Mikiya, additional, Mizoguchi, Akira, additional, Mizusawa, Kanta, additional, Mori, Kenji, additional, Morishita, Fumihiro, additional, Moriyama, Shunsuke, additional, Nagasaki, Hiroshi, additional, Nagata, Shinji, additional, Nakagawa, Yoshiaki, additional, Nakamachi, Tomoya, additional, Niimi, Teruyuki, additional, Ogino, Yukiko, additional, Ogoshi, Maho, additional, Ohira, Tsuyoshi, additional, Ohki-Hamazaki, Hiroko, additional, Ohtaki, Hirokazu, additional, Ohyama, Yoshihiko, additional, Oka, Yoshitaka, additional, Okamoto, Naoki, additional, Osugi, Tomohiro, additional, Park, Min Kyun, additional, Saito, Kazuki, additional, Saito, Yumiko, additional, Sakai, Takafumi, additional, Sakamoto, Hirotaka, additional, Sakamoto, Tatsuya, additional, Satake, Honoo, additional, Sato, Tomomi, additional, Sekiguchi, Toshio, additional, Shimizu, Munetaka, additional, Shinki, Toshimasa, additional, Shinoda, Tetsuro, additional, Sonobe, Haruyuki, additional, Suzuki, Koichi, additional, Suzuki, Nobuo, additional, Tachibana, Tetsuya, additional, Takahashi, Akiyoshi, additional, Takahashi, Toshio, additional, Takei, Yoshio, additional, Takenoya, Fumiko, additional, Takeuchi, Sakae, additional, Tanaka, Yoshiaki, additional, Tanizaki, Yuta, additional, Tsukada, Takehiro, additional, Tsukamoto, Yusuke, additional, Tsutsui, Kazuyoshi, additional, Tsutsui, Naoaki, additional, Ubuka, Takayoshi, additional, Ukena, Kazuyoshi, additional, Wada, Nobuhiro, additional, Watanabe, Jun, additional, Wong, Marty K.S., additional, Xu, Zhifang, additional, Yaginuma, Toshinobu, additional, Yamauchi, Kiyoshi, additional, Yuge, Shinya, additional, and Zitnan, Dusan, additional

Published
2016
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50. FHL5, a novel actin-binding protein, is highly expressed in eel gill pillar cells and responds to wall tension

Author

Mistry, Abinash Chandra, Kato, Akira, Tran, Yen Ha, Honda, Shinji, Tsukada, Takehiro, Takei, Yoshio, and Hirose, Shigehisa

Subjects
Endothelin -- Research, Proteins -- Research, Biological sciences
Abstract

FHL5, a novel actin-binding protein, is highly expressed in eel gill pillar cells and responds to wall tension. Am J Physiol Regul Integr Comp Physiol 287: R1141-R1154, 2004. First published July 29, 2004; doi:10.1152/ajpregu.00108.2004.--Supporting evidence for the contractile nature of fish branchial pillar cells was provided by demonstrating the presence of actin fibers and a novel four-and-a-half LIM (FHL) protein in which expression is specific for contractile tissues and sensitive to the tension applied to the pillar cell. When eel gill sections were stained with rhodamine-phalloidin, a selective fluorescent probe for fibrous actin, a strong bundle-like staining was observed around collagen columns in pillar cells, suggesting the presence of abundant actin fibers. A cDNA clone encoding a novel member of the actin-binding FHL family, FHL5, was isolated from a subtracted cDNA library of eel gill. Northern analysis revealed that FHL5 mRNA is highly expressed only in gills, heart, and skeletal muscle. In gills, FHL5 was found to be confined to pillar cells by immunohistochemistry. Confocal fluorescence microscopy showed that FHL5 is present in both cytosol and nucleus; within the cytosol, a large portion of FHL5 is colocalized with the phalloidin-positive actin bundles. Furthermore, transfection of myogenic C2C12 cells with FHL5 cDNA demonstrated, in addition to its interaction with actin stress fibers, a nuclear shuttling activity of FHL5. The mRNA and protein levels were found to be elevated on 1) transfer of eels from seawater to freshwater, 2) volume expansion by infusion of isotonic dextran-saline, and 3) constriction of gill vasculature by bolus injection of endothelin-1. These results suggest contractile nature of pillar cells and a role of FHL5 in maintaining the integrity and regulating the dynamics of pillar cells. C2C12 myoblast; endothelin; immunohistochemistry; lamella; zinc finger; four-and-a-half LIM

Published
2004

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