1. Lactoferrin inhibits enterovirus 71 infection by binding to VP1 protein and host cells
- Author
-
Huan Yao Lei, Huey Wen Shyu, Tsui Ying Weng, Chun Keung Yu, Jen Ren Wang, Lien Cheng Chen, Shun Hua Chen, and Trai Ming Yeh
- Subjects
Biology ,Antiviral Agents ,Mice ,Cytopathogenic Effect, Viral ,In vivo ,Cell Line, Tumor ,Virology ,Enterovirus Infections ,Enterovirus 71 ,Animals ,Humans ,Enterovirus ,Viral Structural Proteins ,Pharmacology ,Mice, Inbred ICR ,Lactoferrin ,Binding protein ,Ligand binding assay ,Infant ,biology.organism_classification ,Molecular biology ,In vitro ,Animals, Newborn ,Cell culture ,biology.protein ,Cattle ,Antibody - Abstract
The antiviral activities of bovine lactoferrin (LF) against enterovirus 71 (EV71) were studied both in vitro and in vivo. LF protected both human rhabdomyosarcoma and neuroblastoma SK-N-SH cell lines from EV71 infection when it was added at the same time, before, or within 30min after EV71 infection. Using enzyme-linked immunosorbent assay-based binding assay and indirect fluorescent stain, we found that LF could bind to the target cells. Furthermore, it was found that LF could bind to the VP1 protein of EV71, which was blocked in the presence of anti-VP1 antibody. In addition, LF could induce IFN-alpha expression of SK-N-SH cells and inhibit EV71-induced IL-6 production. Finally, LF protected mice against lethal EV71 challenge. Taken together, these results suggest that LF can inhibit EV71 infection by interacting with both EV71 and host cells.
- Published
- 2005
- Full Text
- View/download PDF