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Simple Summary: Toxoplasmosis is a disease caused by the parasite Toxoplasma gondii, which can infect humans and animals, including dogs. Dogs become infected by accidentally ingesting the parasite's oocysts from the environment and eating infected rodents or other small animals. Following infection, dogs develop antibodies against T. gondii. The current study investigated the percentage of dogs living in Greece that have antibodies against the parasite as an indirect way to assess the level of environmental contamination with T. gondii oocysts. Blood samples were collected from 1282 dogs living in both urban and rural regions of Greece, including Attica and Thessaloniki. Overall, 47.6% of the dogs had antibodies against the parasite, indicating they had been exposed to T. gondii. Seropositive dogs were more commonly found in rural areas (53.8%) than in urban areas (43.9%) (p < 0.001). The findings of this study suggest that T. gondii infection is widespread in dogs across Greece, especially in rural regions, and the parasite is highly present in the environment, posing a potential risk for human exposure in these areas. Control measures are necessary to prevent dogs from being infected and to reduce this risk for dog owners. Dog owners should also follow basic hygiene practices, like washing their hands after petting their dogs, to protect themselves. Toxoplasmosis, caused by the protozoan Toxoplasma gondii, is a zoonotic disease that affects various animal species, including dogs, that can serve as sentinels for indirectly estimating the environmental contamination. The current study aimed to determine the seroprevalence of T. gondii IgG and IgM antibodies in dogs across different regions of Greece and assess their living area as a potential risk factor. In total, 1282 blood samples were collected from dogs in urban and rural areas of Greece, including Attica and Thessaloniki. Serum samples were tested for T. gondii-specific IgG and IgM antibodies using an indirect immunofluorescent antibody test. A chi-square test was performed to assess the association between seropositivity for T. gondii and geographical location (urban/rural). The overall T. gondii seroprevalence was 47.6%, while the seroprevalence of IgG and IgM antibodies was 34.3% and 22.2%, respectively. Dogs from rural areas exhibited a significantly higher seroprevalence (53.8%) than those from urban areas (43.9%) (p < 0.001), with the estimated odds ratio being equal to 1.49 (95% CI, 1.18 to 1.65) and the relative risk increased by 22.4%. Dogs in Greece are highly exposed to T. gondii, particularly in rural areas. Measures to prevent canine infections are necessary, and basic hygiene practices, such as hand washing after petting dogs, are required to reduce human infection risk and safeguard public health. [ABSTRACT FROM AUTHOR]

The exposure of dairy cows to Coxiella burnetii using molecular and serological techniques was investigated in this study. Bulk tank milk and serum samples were collected from various farms in Greece (mainly northern Greece). DNA extraction was performed on milk samples, and qPCR targeting the IS1111 insertion sequence was performed to detect bacterial pathogens. An ELISA was used to detect specific antibodies in bulk milk and individual serum samples. Data on farms were collected in the field using handheld Global Positioning System Garmin units. The collected data were analyzed using an Ecological Niche Model within the framework of a geographic information system. The results indicated that in more than half of the dairy farms (35/60, 58.3%), C. burnetii is present in milk. Specific antibodies were also detected in almost all milk samples (57/60, 95.0%). At least one seropositive animal was identified using ELISA in the majority of the examined farms (25/28, 89.3%). C. burnetii PCR-positive farms were located in the low-altitude zone with a mean value of 97 m above sea level (range: 2–681). The environmental variable with the highest gain when used in isolation is precipitation in the wettest quarter (28.3% contribution), which therefore appears to have the most useful information by itself. The environmental variable that decreases the gain the most when omitted is the minimal temperature of the coldest month (6.9% contribution). The analysis demonstrated that a mild climate with low precipitation favors a positive status. The exposure of dairy cattle farms to C. burnetii is massive, raising significant concerns regarding livestock production and public health implications. [ABSTRACT FROM AUTHOR]

West Nile virus (WNV) is the etiologic agent of an ongoing human disease outbreak in Greece since 2010. Until 2014, successive yearly outbreaks occurred mainly in central and northeastern Greece [...]

The aim of this study was to investigate the occurrence of Bartonella spp, Brucella spp, Coxiella burnetii , and Francisella tularensis in European Brown hares ( Lepus europaeus ) hunter harvested during 2-year hunting periods in northern and central Greece. Serum samples were examined for the presence of IgG antibodies by using an immune fluorescence test and/or an enzyme-linked immunosorbent assay. PCR was used to detect Bartonella spp DNA in blood samples and Brucella spp, C. burnetii , and F. tularensis DNA in liver samples. Antibodies against Bartonella spp were detected in 12 hares (12/105); whereas none of the hares examined was seropositive for Brucella spp , C. burnetii , and F. tularensis. The presence of Bartonella spp, Brucella spp, C. burnetii , and F. tularensis DNA was not detected in the samples examined. This study did not provide any evidence that the European Brown hare is involved in the epidemiology of Brucella spp , C. burnetii , and F. tularensis in Greece. However, our results suggest that this species is exposed to Bartonella spp, which gives the impetus for further investigation of its role as another host of this bacterium.