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Purpose: Artificial intelligence (AI) may be used to learn and predict the maxillomandibular relationship, particularly when the number of occluding teeth pairs is insufficient. This study aimed to investigate the feasibility of training a new two-stage coarse-to-fine teeth alignment pipeline AI system in predicting maxillomandibular relationships based on the occlusal morphology of antagonistic teeth., Methods: Maxillary and mandibular stone casts were collected and scanned at the maximal intercuspal position (MIP). A deep learning alignment network was trained using 90% of cast pairs. The remaining 10% of pairs were input into the trained AI system for validation. The maxillomandibular relationships predicted by the AI system were superimposed and compared with those of the mounted casts. Cartesian x-, y-, and z-coordinates were defined for each mandibular tooth scan with respect to (w.r.t.) its occlusal plane and dental midline. The discrepancy in the position of maxillary teeth scans was described based on rotation and translation., Results: A total of 325 pairs of maxillary and mandibular stone casts were collected, with 300 pairs used for training and 25 for validation. For the AI-predicted maxillomandibular relationship, the mean rotational discrepancies w.r.t. the x-, y-, and z-axis were 1.407°±1.548°, 1.269°±8.476°, and 0.730°±1.334°, respectively. The mean translational discrepancies w.r.t. the x-, y-, and z-axis were 0.185±1.324 mm, 1.222±0.848 mm, -1.034±0.273 mm, respectively., Conclusions: The AI-predicted maxillomandibular relationship for maxillary and mandibular teeth scans shows discrepancies of less than 1.3 mm and 1.5° compared to the actual relationships.

Diamond is an exceptional material with great potential across various fields owing to its interesting properties 1,2 . However, despite extensive efforts over the past decades 3-5 , producing large quantities of desired ultrathin diamond membranes for widespread use remains challenging. Here we demonstrate that edge-exposed exfoliation using sticky tape is a simple, scalable and reliable method for producing ultrathin and transferable polycrystalline diamond membranes. Our approach enables the mass production of large-area (2-inch wafer), ultrathin (sub-micrometre thickness), ultraflat (sub-nano surface roughness) and ultraflexible (360° bendable) diamond membranes. These high-quality membranes, which have a flat workable surface, support standard micromanufacturing techniques, and their ultraflexible nature allows for direct elastic strain engineering and deformation sensing applications, which is not possible with their bulky counterpart. Systematic experimental and theoretical studies reveal that the quality of the exfoliated membranes depends on the peeling angle and membrane thickness, for which largely intact diamond membranes can be robustly produced within an optimal operation window. This single-step method, which opens up new avenues for the mass production of high-figure-of-merit diamond membranes, is expected to accelerate the commercialization and arrival of the diamond era in electronics, photonics and other related fields., Competing Interests: Competing interests: Z.C., J.J., Q.W., Z.W. and K.H.L. are inventors on a Chinese invention patent related to this work (no. 202310540694.7) entitled 'Scalable fabrication of large-scale, ultraflat and transferable polycrystalline diamond membranes'. Z.C., J.J., Y. Lin, Q.W., Z.W. and K.H.L. are inventors on a Chinese invention patent related to this work (no. 202310990328.1) entitled 'A method for fabricating in large-scale ultraflat polycrystalline diamond membranes'. The other authors declare no competing interests., (© 2024. The Author(s), under exclusive licence to Springer Nature Limited.)

Objectives: Maintenance of oral muscle functions is important for survival and communication. Utilizing Artificial Intelligence (AI) as a self-health-management material has shown promise. Here we developed a functional and AI-enabled smartphone e-Oral APP that provided real-time feedback features to let humans collaborate with AI, targeting to improve oral biomechanical and speech functions of adults., Methods: A total of 113 neurologically healthy Hong Kong citizens over 50 years old were recruited in this study from June to October 2022. A set of 12 exercises of the tongue, lips, and jaw were conducted with guidance provided in the self-developed e-Oral APP, in which visual-audio demonstrations and immediate feedback on facial movements by an open-source AI-pretrained 68-point Ensemble of Regression Tree (ERT) face landmark detector was given to the users. After 8-week exercises, assessments after 1-week and 4-week post-training were conducted. Primary outcomes were measured on oral functions, including occlusal force, masticatory efficiency, tongue strength, along with dry mouth condition, and oral health-related quality of life (OHQoL), while secondary outcomes were comprised of oral diadochokinesis (DDK) and swallowing ability. The usability of the APP was evaluated by a self-written questionnaire., Results: After exercising a set of 12 exercises on the tongue, lips, and jaw for 8 weeks, 70 out of 113 participants (average age of 67.70±4.93 y.o., 52 female and 18 male) had a significant improvement in the occlusal force (p < 0.001), while masticatory efficiency (p = 0.002), tongue pressure (p < 0.001) and endurance (p = 0.004) were also improved. 80.3 % of respondents rated the APP with an overall rating of 3 out of 5 points, and 68.6 % would recommend the APP to others., Conclusions: AI-based APP can be an effective approach to help healthy adults improve their occlusal force, masticatory efficiency, tongue functions, and oral diadochokinesis after 8-week home oral exercises. Furthermore, these improvements can be sustained for at least four weeks., Clinical Significance: This is the first AI-assisted APP developed for oral muscle training. Our findings demonstrated that a self-administrated human-AI collaboration APP can improve clinically oral muscle biomechanics and functions for healthy adults. AI technology in smartphone gadgets provides a cost-effective, convenient, and reliable means for oral muscles training for adults., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: James Kit Hon Tsoi reports financial support was provided by Nakao Foundation. James Kit Hon Tsoi reports a relationship with Nakao Foundation that includes: funding grants. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Objectives: To proof the feasibility of direct colour printing on 3Y-TZP using 222 nm UV-C through investigating the degree and durability of the colour changes, and testifying whether surface, mechanical and biological properties are influenced by the treatment., Methods: 222 nm UV-C light (Irradiance: 1.870 mW/cm 2 ) was used to treat 3Y-TZP for durations from 15 min to 24 h. ΔE*, TP, crystalline structure, surface morphology, S a , BFS and biological activities were investigated before and after irradiation. SPSS 28.0 was used for statistical analysis (α = 0.05)., Results: 222 nm UV-C irradiation was capable to shade white 3Y-TZP into tooth colours. With the increase of ΔE*, TP decreased, such that the longer the irradiation time, the higher the ΔE* (logarithmic relationship) and lower the TP. Despite the induced optical changes being prone to fade, the process can be predicted by inversely proportional relationships between ΔE*, TP and the testing points. The treated surface exhibited enhanced hydrophilicity, while the recovery phenomenon was observed. Other properties were not altered by the treatment., Significance: This is the seminal study demonstrating the feasibility of direct colour printing on 3Y-TZP using 222 nm UV-C. The new relationship between the colour centre and E g of 3Y-TZP was established, whereas the induced optical changes were stabilised after a certain period and were highly predictable by controlling the irradiation periods. The irradiation was only correlated to the electron excitation and oxygen vacancies, and would not lead to any changes of other properties. A simple, safe and promising approach to achieve satisfactory colours on 3Y-TZP in clinical practice can be developed., Competing Interests: Declaration of Competing Interest There are no known conflicts of interest associated with this publication and there has been no significant financial support for this work that could have influenced its outcome., (Copyright © 2024 Elsevier Inc. All rights reserved.)

Automated tooth segmentation and identification on dental radiographs are crucial steps in establishing digital dental workflows. While deep learning networks have been developed for these tasks, their performance has been inferior in partially edentulous individuals. This study proposes a novel semi-supervised Transformer-based framework (SemiTNet), specifically designed to improve tooth segmentation and identification performance on panoramic radiographs, particularly in partially edentulous cases, and establish an open-source dataset to serve as a unified benchmark. A total of 16,317 panoramic radiographs (1589 labeled and 14,728 unlabeled images) were collected from various datasets to create a large-scale dataset (TSI15k). The labeled images were divided into training and test sets at a 7:1 ratio, while the unlabeled images were used for semi-supervised learning. The SemiTNet was developed using a semi-supervised learning method with a label-guided teacher-student knowledge distillation strategy, incorporating a Transformer-based architecture. The performance of SemiTNet was evaluated on the test set using the intersection over union (IoU), Dice coefficient, precision, recall, and F1 score, and compared with five state-of-the-art networks. Paired t -tests were performed to compare the evaluation metrics between SemiTNet and the other networks. SemiTNet outperformed other networks, achieving the highest accuracy for tooth segmentation and identification, while requiring minimal model size. SemiTNet's performance was near-perfect for fully dentate individuals (all metrics over 99.69%) and excellent for partially edentulous individuals (all metrics over 93%). In edentulous cases, SemiTNet obtained statistically significantly higher tooth identification performance than all other networks. The proposed SemiTNet outperformed previous high-complexity, state-of-the-art networks, particularly in partially edentulous cases. The established open-source TSI15k dataset could serve as a unified benchmark for future studies.

Objective: The commonly used base monomer utilized in resinous commercial dental restorative products is bis-GMA which is derived from bisphenol-A (BPA) - a well-known compound which may disrupt endocrine functions. To address concerns about its leaching into the oral environment and to optimize the quality of dental composites, a BPA-free alternative base monomer, fluorinated urethane dimethacrylate (FUDMA), was designed by modifying a UDMA monomer system., Methods: Nine groups of composites were prepared by mixing the base monomers and TEGDMA in a ratio of 70/30 wt% to which were added silanized glass particles (mean diameter: 0.7 µm) in 3 different volume fractions (40, 45, and 50 vol%). Bis-GMA and UDMA base monomers were used as control groups in the same ratios. Various properties including degree of conversion (DC), flexural strength (FS) and flexural modulus (FM), water sorption (WS), solubility (SL), surface hardness and roughness, and initial adhesion property against S.mutans were investigated. One-way analysis of variance followed by Bonferroni test at α = 0.05 was used to analyze the results., Results: A significant difference in FS between FUDMA-based composite with 40 vol% filler (120.3 ± 10.4 MPa) and Bis-GMA-based composite with the same filler fraction (105.8 ± 10.0 MPa) was observed but there was no significant difference among other groups. The UDMA based group exhibited the highest WS (1.3 ± 0.3 %). Bis-GMA showed greater initial bacterial adhesion but was not statistically different from the other groups (p = 0.082)., Significance: FUDMA-based resin composites exhibit comparable mechanical and bacterial adhesion properties compared with Bis-GMA and UDMA-based composites. The FUDMA composites show positive outcomes indicating they could be used as substitute composites to Bis-GMA-based composites., (Copyright © 2024 Elsevier Inc. All rights reserved.)

Background: This study compared the in vitro erosive effects of 2 western and 2 Chinese medicinal over-the-counter paediatric medications on primary teeth in a continuous time scale., Methods: Twenty enamel blocks obtained from 10 deciduous incisors were randomly assigned into 5 groups (n = 4 each): Group GA and Group GB-paracetamol liquid (Jean-Marie Pharmacal Co. Ltd and Universal Pharmaceutical Laboratories Ltd, respectively); Group GC and Group GD-reconstituted Chinese medicinal granules (Huarun 999 and Guizhou Bailing Group Pharmaceutical Co. Ltd, respectively); and deionised water (control group). The pH levels of these liquids were assessed. The immersion process was repeated for 20 rounds. For each round, each sample was immersed in liquid for 15 seconds and then rinsed with deionised water for 15 seconds. Mean hardness ratio (MHR) at 5, 10, 15, and 20 rounds was evaluated using Vickers microhardness test. The initial and final surface morphology and chemistries were analysed by scanning electron microscopy (SEM) and energy-dispersive x-ray spectroscopy (EDS)., Results: All 4 liquid medications have shown acidic pH (4.74-5.76). After 20 rounds of immersion, the MHR 20 of the GA (0.886) and GB (0.930) groups decreased significantly from the original MH 0 . The GC (0.978) and GD (0.985) groups illustrated a similar trend as the deionised water control group (0.985). Despite EDS, chemical analysis showed an increase of Ca/C mole ratio after drug immersion, and no significant difference (P > .05) was found on the ratios Ca/P and Ca/C and the amounts of magnesium or sodium before and after immersion according to the Wilcoxon signed-rank analysis. Distinctive enamel loss with irregular craters, and a corroded surface with fracture lines along the border of prism head were observed in the SEM of groups GA and GB, respectively. However, findings in both Chinese medicine groups were similar, such that erosive changes were barely seen, similar to the control group., Conclusions: This study concluded that the pH of liquid medication may not be a significant factor that contributes to enamel softening. Traditional Chinese granule medications may be a safer option for primary teeth., Competing Interests: Conflict of interest None disclosed., (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)

Objectives: To evaluate the mechanical, wear, antibacterial properties, and biocompatibility of injectable composite materials., Methods: Two injectable composite resins (GU and BI), one flowable composite resin (FS), and one flowable compomer (DF), in A2 shade, were tested. Mechanical properties were tested via three-point bending test immediately after preparation and after 1-day, 7-day, 14-day, and 30-day water storage. Under water-PMMA slurry immersion, specimens were subjected to a 3-body wear test (10,000 cycles) against stainless steel balls, while the roughness, wear depth, and volume loss were recorded. After 1-day and 3-day MC3T3-E1 cell culture, cell viability was evaluated with CCK-8 test kits, while the cell morphology was observed under CLSM and SEM. Antibacterial properties on S. mutans were assessed via CFU counting, CLSM, and SEM observation. SPSS 26.0 was used for statistical analysis (α = 0.05)., Results: The mechanical properties were material-dependent and sensitive to water storage. Flexural strength ranked GU > FS > BI > DF at all testing levels. Three nanocomposites had better wear properties than DF. No significant difference on 1-day cell viability was found, but DF showed significantly lower cell proliferation than nanocomposites on 3-day assessment. GU and FS had more favourable cell adhesion and morphology. CFU counting revealed no significant difference, while FS presented a slightly thicker biofilm and BI showed relatively lower bacteria density., Conclusions: Injectable nanocomposites outperformed the compomer regarding mechanical properties, wear resistance, and biocompatibility. The tested materials presented comparable antibacterial behaviours. Flowable resin-based composites' performances are affected by multiple factors, and their compositions can be attributed., Clinical Significance: A profound understanding of the mechanical, wear, and biological properties of the restorative material is imperative for the clinical success of dental restorations. The current study demonstrated superior properties of highly filled injectable composite resins, which imply their wider indications and better long-term clinical performances., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: This study is partially funded, with the materials supported, by GC Corporation., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Objectives: To investigate and compare the chemical and optical stability of four restorative composite materials: two injectable resins, one flowable resin and one compomer., Methods: Two injectable nano-filled composite resins: G-aenial Universal (GU) and Beautifil Injectable XSL (BI), a flowable composite resin: Filtek Supreme Flowable (FS) and a compomer: Dyract Flow (DF), in A2 shade were tested and compared. Water sorption and solubility were conducted according to ISO4049:2019 standard; ICP-OES and F-ion selective electrode were used to test the elemental release; Degree of conversion (DC) was obtained by using FTIR; water contact angle was obtained by static sessile drop method, and a spectrophotometer was used for optical properties (ΔE ⁎ , ΔL ⁎ and TP). SPSS 28.0 was used for statistical analysis and the significant level was pre-set as α = 0.05., Results: GU performed the best in water sorption and solubility, FS had the lowest elemental release, the best colour stability, and the highest DC IM and DC 24-h . DF, the compomer had the lowest, and GU and BI, the injectable composites had the largest water contact angle, respectively. Correlations were found between water sorption and water solubility., Conclusions: The four composite restorative materials showed different chemical and optical behaviours. Overall, composite resins performed better than compomer, while additional laboratory and in vivo tests are necessary to obtain a more comprehensive comparison between injectable and flowable composite resins. W sp and W sl are influenced by many common factors, and the values are highly positively related., Clinical Significance: A comprehensive understanding of materials is crucial before selecting materials for clinical practice. Composite resins rather than compomers are recommended because of their exceptional properties, which make them eligible for a wide range of clinical applications and an elongated lifespan., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: This study is partially funded, and the materials were provided, by GC Corporation., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

This study aims to investigate whether toothbrushes with fluoride-infused bristles have any (re)mineralisation effects on bovine enamel. Bovine incisors (N = 160) were extracted, and the buccal side of the crown was cut into dimensions of ~5 mm × 5 mm with a low-speed saw. These specimens were randomly allocated into four groups: half (80 teeth) were stored in demineralising solution (DM), and the other half were stored in deionised water (DW) for 96 h. Then, they were brushed with a force of 2.0 ± 0.1 N for five min with a manual toothbrush with either fluoride-infused (TF) or regular (TR) bristles. Microhardness (Vickers), X-ray diffraction (XRD), energy-dispersive X-ray spectroscopy (EDS), and scanning electron microscopy (SEM) were used to investigate the surfaces of the bovine enamel specimens before and after brushing. Two-way ANOVA was used to analyse the hardness data, and the pairwise comparison method was used to analyse the Ca/P ratio, for each group at α = 0.05. The results show that brushing with either of these toothbrushes increased the Vickers microhardness on DM and DW enamel ( p < 0.001), whereas hydroxyapatite was revealed in all groups by XRD. The DM samples showed a significant increase ( p < 0.05) in the Ca/P ratios after brushing with TR and TF. Conversely, under DW conditions, these ratios decreased significantly after brushing. In terms of the F atomic%, TF increased significantly. SEM revealed mineral deposition in the DM groups after toothbrushing. To conclude, toothbrushing effectively induces the microhardness of sound and demineralised enamel, while fluoride-infused bristles might be able to retain fluoride on the enamel surface.

This study aims to develop multi-functional bio-safe dental resin composites with capabilities for mineralization, high in vitro biocompatibility, and anti-biofilm properties. To address this issue, experimental resin composites consisting of UDMA/TEGDMA-based dental resins and low quantities (1.9, 3.8, and 7.7 vol%) of 45S5 bioactive glass (BAG) particles were developed. To evaluate cellular responses of resin composites, MC3T3-E1 cells were (1) exposed to the original composites extracts, (2) cultured directly on the freshly cured resin composites, or (3) cultured on preconditioned composites that have been soaked in deionized water (DI water), a cell culture medium (MEM), or a simple HEPES-containing artificial remineralization promotion (SHARP) solution for 14 days. Cell adhesion, cell viability, and cell differentiation were, respectively, assessed. In addition, the anti-biofilm properties of BAG-loaded resin composites regarding bacterial viability, biofilm thickness, and biofilm morphology, were assessed for the first time. In vitro biological results demonstrated that cell metabolic activity and ALP expression were significantly diminished when subjected to composite extracts or direct contact with the resin composites containing BAG fillers. However, after the preconditioning treatments in MEM and SHARP solutions, the biomimetic calcium phosphate minerals on 7.7 vol% BAG-loaded composites revealed unimpaired or even better cellular processes, including cell adhesion, cell proliferation, and early cell differentiation. Furthermore, resin composites with 1.9, 3.8, and 7.7 vol% BAG could not only reduce cell viability in S. mutans biofilm on the composite surface but also reduce the biofilm thickness and bacterial aggregations. This phenomenon was more evident in BAG7.7 due to the high ionic osmotic pressure and alkaline microenvironment caused by BAG dissolution. This study concludes that multi-functional bio-safe resin composites with mineralization and anti-biofilm properties can be achieved by adding low quantities of BAG into the resin system, which offers promising abilities to mineralize as well as prevent caries without sacrificing biological activity.

Objectives: The aim of the present study was to examine the plaque removal effectiveness of a personalised 3D-printed dental plaque removal mouthguard device in a clinical trial setting., Methods: A personalised 3D-printed mouthguard was developed to clean dental plaque using micro-mist. A clinical trial was conducted to examine the plaque removal effectiveness of this device. The clinical trial recruited 55 participants (21 males and 34 females) with an average age of 68.4 years (range, 60-81 years). Dental plaque was dyed by plaque disclosing liquid (Ci). Turesky Modification of the Quigley-Hein Plaque Index (TMQHPI) was used to evaluate the level and rate of plaque formation on the tooth surface. The TMQHPI was recorded and intraoral photos were taken before and after mouthguard cleaning. The plaque removal rate was calculated based on TMQHPI and intraoral photos (pixel-based method) before and after cleaning., Results: The personalised 3D-printed micro-mist injection mouthguard can be effective in dental plaque removal on tooth and gingiva, and the effectiveness lies between that of a manual toothbrush and a mouth rinse. The newly proposed pixel-based method can be a practical, high sensitive tool to evaluate the level of plaque formation., Conclusions: Under the conditions of the present study, we conclude that the personalised 3D-printed micro-mist injection mouthguard can be useful in reducing dental plaque and may be especially suitable for older adults and disabled people., Competing Interests: Conflict of interest None disclosed., (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)

The purpose of the study was to determine the influence of preparation techniques on marginal adaptation and sealing of Biodentine™ and TotalFill® RRM bioceramic retrograde fillings. Fifty-two single-root teeth extracted for periodontal reasons were used. Root canals were instrumented using Reciproc Blue #25 and obturated using a single cone technique with an AH Plus® root canal sealer. Retrograde cavities were prepared with Piezomed device (Piezo), Er:YAG laser in short-pulse(SP) and quantum square pulse(QSP) modes and filled with Biodentine™ (BD) or TotalFill® RRM (TF). There were 6 groups (n=8): (1) Piezo BD, (2) Piezo TF, (3) SP BD, (4) SP TF, (5) QSP BD, and (6) QSP TF, and positive and negative controls (n=2). Micro-CT analysis was performed on two samples from each group. Percentage volumes of internal and external voids in apical 1.5 mm were determined. Rhodamine B dye leakage was done on six samples. The samples were cut longitudinally and examined under a stereomicroscope. Digital recordings were analyzed in ImageJ software. The deepest penetration of color in mm was recorded. The data were statistically analyzed using ANOVA and Duncan's test at the level of significance α=0.05. TotalFill® RRM performed significantly better than Biodentine™ in terms of sealing (p<0.05) and marginal adaptation, as evaluated by micro-CT. Sealing was significantly better in SP compared to QSP mode preparations (p<0.05). Differences between Piezomed and laser modes were not significantly different (p>0.05). Sealing was statistically significantly better with TotalFill® RRM compared to Biodentine™ and in Er:YAG SP preparations compared to Er:YAG QSP., (© 2023. The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature.)

Background: Autogenous tooth bone graft material (AutoBT) has been advocated as a bone substitute when conducting alveolar ridge preservation. This study is aimed at using a radiomics approach in order to evaluate and testify whether AutoBT can stimulate bone growth during socket preservation in severe periodontal cases., Materials and Methods: For this study, 25 cases with severe periodontal diseases were selected. The patients' AutoBTs were inserted into the extraction sockets and covered with Bio-Gide ® collagen membranes. 3D CBCT scans and 2D X-rays were taken of the patients before surgery and after 6 months post-surgery. For the retrospective radiomics analysis, the maxillary and mandibular images were compared in different groups. Maxillary bone height was analyzed at the buccal, middle, and palatal crest sites, while the mandibular bone height was compared at the buccal, center, and lingual crest sites., Results: In the maxilla, the alveolar height was increased by -2.15 ± 2.90 mm at the buccal crest; -2.45 ± 2.36 mm at the center of the socket, and -1.62 ± 3.19 mm at the palatal crest, while the height of the buccal crest was increased by 0.19 ± 3.52 mm, and the height at the center of the socket was increased by -0.70 ± 2.71 mm in the mandible. The three-dimensional radiomics analysis demonstrated significant bone growth in the local alveolar height and high density., Conclusion: Based on clinical radiomics analysis, AutoBT could be used as an alternative bone material in socket preservation after tooth extraction in patients with severe periodontitis.

Quaternary ammonium compounds (QACs) have been widely used due to their excellent antimicrobial activity. However, using the technology where nanomaterials are employed as drug carriers to deliver QAC drugs has not been fully explored. In this study, mesoporous silica nanoparticles (MSNs) with short rod morphology were synthesized in a one-pot reaction using an antiseptic drug cetylpyridinium chloride (CPC). CPC-MSN were characterized via various methods and tested against three bacterial species ( Streptococcus mutans , Actinomyces naeslundii , and Enterococcus faecalis ), which are associated with oral infections, caries, and endodontic pathology. The nanoparticle delivery system used in this study prolonged the release of CPC. The manufactured CPC-MSN effectively killed the tested bacteria within the biofilm, and their size allowed them to penetrate into dentinal tubules. This CPC-MSN nanoparticle delivery system demonstrates potential for applications in dental materials.

Objectives: This study utilised an Artificial Intelligence (AI) method, namely 3D-Deep Convolutional Generative Adversarial Network (3D-DCGAN), which is one of the true 3D machine learning methods, as an automatic algorithm to design a dental crown., Methods: Six hundred sets of digital casts containing mandibular second premolars and their adjacent and antagonist teeth obtained from healthy personnel were machine-learned using 3D-DCGAN. Additional 12 sets of data were used as the test dataset, whereas the natural second premolars in the test dataset were compared with the designs in (1) 3D-DCGAN, (2) CEREC Biogeneric, and (3) CAD for morphological parameters of 3D similarity, cusp angle, occlusal contact point number and area, and in silico fatigue simulations with finite element (FE) using lithium disilicate material., Results: The 3D-DCGAN design and natural teeth had the lowest discrepancy in morphology compared with the other groups (root mean square value = 0.3611). The Biogeneric design showed a significantly (p < 0.05) higher cusp angle (67.11°) than that of the 3D-DCGAN design (49.43°) and natural tooth (54.05°). No significant difference was observed in the number and area of occlusal contact points among the four groups. FE analysis showed that the 3D-DCGAN design had the best match to the natural tooth regarding the stress distribution in the crown. The 3D-DCGAN design was subjected to 26.73 MPa and the natural tooth was subjected to 23.97 MPa stress at the central fossa area under physiological occlusal force (300 N); the two groups showed similar fatigue lifetimes (F-N curve) under simulated cyclic loading of 100-400 N. Designs with Biogeneric or technician would yield respectively higher or lower fatigue lifetime than natural teeth., Significance: This study demonstrated that 3D-DCGAN could be utilised to design personalised dental crowns with high accuracy that can mimic both the morphology and biomechanics of natural teeth., (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)

This study was conducted as a means to evaluate the stress distribution patterns of anterior ceramic resin-bonded fixed partial dentures derived from different materials and numerous connector designs that had various loading conditions imposed onto them through the utilization of the finite element method. A finite element model was established on the basis of the cone beam computed tomography image of a cantilevered resin-bonded fixed partial denture with a central incisor as an abutment and a lateral incisor as a pontic. Sixteen finite element models representing different conditions were simulated with lithium disilicate and zirconia. Connector height, width, and shape were set as the geometric parameters. Static loads of 100 N, 150 N, and 200 N were applied at 45 degrees to the pontic. The maximum equivalent stress values obtained for all finite element models were compared with the ultimate strengths of their materials. Higher load exhibited greater maximum equivalent stress in both materials, regardless of the connector width and shape. Loadings of 200 N and 150 N that were correspondingly simulated on lithium disilicate prostheses of all shapes and dimensions resulted in connector fractures. On the contrary, loadings of 200 N, 150 N, and 100 N with rectangular-shaped connectors correspondingly simulated on zirconia were able to withstand the loads. However, two of the trapezoidal-shaped zirconia connectors were unable to withstand the loads and resulted in fractures. It can be deduced that material type, shape, and connector dimensions concurrently influenced the integrity of the bridge.

Objective: To seek dentine analogue materials in combined experimental, analytical, and numerical approaches on the mechanical properties and fatigue behaviours that could replace human dentine in a crown fatigue laboratory test., Methods: A woven glass fibre-filled epoxy (NEMA grade G10; G10) and a glass fibre-reinforced polyamide-nylon (30% glass fibre reinforced polyamide-nylon 6,6; RPN) were investigated and compared with human dentine (HD). Flexural strength and elastic modulus (n = 10) were tested on beam-shaped specimens via three-point bending, while indentation hardness (n = 3) was tested after fracture. Abutment substrates of G10, RPN and HD were prepared and resin-bonded with monolithic lithium disilicate crowns (n = 10), then subjected to wet cyclic loading in a step-stress manner (500 N initial load, 100 N step size, 100,000 cycles per step, 20 Hz frequency). Data were statistically analysed using Kruskal-Wallis one-way ANOVA followed by post-hoc comparisons (α = 0.05). Survival probability estimation was performed by Mantel-Cox Log-Rank test with 95% confidence intervals. The fatigue failure load (FFL) and the number of cycles until failure (NCF) were evaluated with Weibull statistics. Finite Element Models of the fatigue test were established for stress distribution analysis and lifetime prediction. Fractographic observations were qualitatively analysed., Results: The flexural strength of HD (164.27 ± 14.24 MPa), G10 (116.48 ± 5.93 MPa), and RPN (86.73 ± 3.56 MPa) were significantly different (p < 0.001), while no significant difference was observed in their flexural moduli (p = 0.377) and the indentation hardness between HD and RPN (p = 0.749). The wet cyclic fatigue test revealed comparable mean FFL and NCF of G10 and RPN to HD (p = 0.237 and 0.294, respectively) and similar survival probabilities for the three groups (p = 0.055). However, RPN promotes higher stability and lower deviation of fatigue test results than G10 in Weibull analysis and FEA., Significance: Even though dentine analogue materials might exhibit similar elastic properties and fatigue performance to human dentine, different reliabilities of fatigue on crown-dentine analogues were shown. RPN seems to be a better substrate that could provide higher reliability and predictability of laboratory study results., (Copyright © 2022 Elsevier Inc. All rights reserved.)

Background: The aim of the study was to investigate whether the citric acid and rough surface have a synergistic effect leading to severe wear behavior of resin composite., Materials and Methods: Disk-shaped (Ø15 × 1.5 mm) specimens of resin composite (n = 12) with different initial roughness were prepared. Reciprocating ball-on-flat wear tests were performed under distilled water and citric acid (pH = 5.5) respectively. The coefficient of friction (COF), wear volume loss, and duration of the running-in period were quantified to assess the wear performance. And the values were analyzed with one-way ANOVA (α = 0.05). Regression analysis was applied to examine the influence of Ra values and mediums on the wear data. The wear morphology was analyzed by scanning electron microscopy and a 3D profilometer., Results: The average COF was higher in distilled water than in citric acid but was independent of the surface roughness. For the composite, the volume loss of worn area and running-in period increased with surface roughness when tested under distilled water. However, these increasing trends were not found in citric acid. All specimens exhibited mild wear behavior with low COF and less superficial abrasion in acidic medium., Conclusions: The effect of initial roughness on wear behavior depends on the medium. In distilled water, resin composites with high initial roughness exhibit a longer running-in time, which eventually leads to a significant increase in material loss. The adverse effects of high roughness can be alleviated by the lubrication of citric acid, which can maintain a mild wear behavior regardless of initial surface roughness., (© 2022. The Author(s).)

This review aims to provide a comprehensive analysis of the characterizations of bioactive glass (BAG)-loaded dental resin-based composite materials. Online databases (Web of Science, PubMed, and Science Direct) were used to collect data published from January 2011 to January 2022. Only BAG-containing resin adhesive and resin restorative composites are discussed in this narrative review. BAG-loaded resin composites exhibit excellent mineralization ability reflecting enhanced ion release, pH elevation, and apatite formation, especially regarding high BAG loading. This aids the anti-demineralization and remineralization of teeth. Furthermore, BAG-loaded resin composites demonstrated in vitro biocompatibility and antibacterial performance. It has been suggested that BAG fillers with small particle sizes and no more than 20 wt% in terms of loading amount should be used to guarantee the appropriate mechanical properties of resin composites. However, most of these studies focused on one or some aspects using different resin systems, BAG types, and BAG amounts. As such, this makes the comparison difficult, and it is essential to find an optimal balance between different properties. BAG-loaded resin composites can be regarded as bioactive materials, which present major benefits in dentistry, especially their capability in the bacterial inhibition, cell biocompatibility, anti-demineralization, and remineralization of teeth.

Purpose: This study explores novel solutions other than standard SBF for biomimetic evaluations of mineralization particularly for resin composites containing bioactive glass (BAG)., Methods: Experimental UDMA/TEGDMA resin composites with 0.0, 1.9, 3.8 or 7.7 vol% of 45S5 BAG fillers were prepared. Besides simulated body fluid (SBF) as control, the specimens were immersed in three other solutions either with bicarbonate which are Hank's balanced salt solution (HBSS) and cell culture medium (MEM), or without bicarbonate which is a novel Simple HEPES-containing Artificial Remineralization Promotion (SHARP) solution, for 3, 7 and 14 days. These solutions were then analyzed by ICP-OES and pH meter, and the surfaces of the BAG composites were analyzed by SEM, XRD and FTIR., Results: ICP-OES revealed Ca and P concentration continuously decrease, while Si concentration increases with time in the solutions other than SBF, which showed almost unchanged elemental concentration. Only SHARP solution is able to maintain a constant pH over the immersion time. SEM, together with XRD and FTIR, showed nano-sized octacalcium phosphate (OCP) nanospheres formation on 3.8 and 7.7 vol% BAG composites after 14 days immersion in HBSS (500-600 nm) and MEM (300-400 nm). SHARP solution enabled OCP formation after 3 days and then self-assembled into urchin-like carbonated hydroxyapatite (CHA) microspheres encompassed with nanorods of 100 nm width and 8 µm length after 14 days of immersion for 7.7 vol% BAG composites., Conclusion: This study suggests SHARP solution can evaluate mineralization biomimetically whereas CHA microspheres can be formed on BAG-containing resin composites.

Purpose: To investigate the error accumulation and distribution through various stages of the digital workflow for complete-arch implant-supported framework fabrication., Materials and Methods: A resin model of edentulous maxilla with 6 dental implants was scanned using an intraoral scanner for 10 times (Complete-digital group). Ten conventional gypsum casts were made and digitized by a laboratory scanner (Analogue-digital group). Five implant frameworks were designed and milled using CAD-CAM technique for each workflow. Inter-implant distances and angles of the resin model (reference) and frameworks were measured by a coordinate measuring machine, while the scans and virtual frameworks were examined by an inspection software. Effect of type of workflow and the individual stage on the accuracy of the frameworks were analysed by Two-way ANOVA., Results: The expanded uncertainty of both workflows was ~150 μm and ~ 0.8°. The accuracy of the CAD stage was the highest. In the complete-digital workflow, the greatest distortion was found in the data acquisition stage, while in the analogue-digital workflow, it was found in the CAM stage. Compared with the analogue-digital group, the complete-digital group showed a significant higher precision in the first quadrant, but lower trueness in the second quadrant in data acquisition, and a significantly lower precision in the second quadrant at the CAD stage., Conclusions: Linear distortions of the complete-digital and analogue-digital workflows were clinically acceptable, while angular distortions were not. Distortions were generally derived from data acquisition and CAM stage. The CAD precision depended on the distortions derived from data acquisition. The complete-digital workflow was not as accurate as the analogue-digital in complete-arch implant rehabilitation., (© 2022 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)

Background: A continuous positive airway pressure (CPAP) mask is a respiratory ventilation method used for treating breathing disorders including respiratory failure and obstructive sleep apnoea (OSA). The forces applied by a CPAP mask may affect facial development and lead to pressure ulcers. In an experimental setting, the magnitude and the distribution of the contact pressures developed by a CPAP mask on the face were investigated for providing information aiming at optimizing the design of the device., Materials and Methods: A nasal CPAP mask with forehead support was placed via its headgear straps on a rigid phantom head and then a controlled load was incrementally applied via a mechanical testing system (5848 Micro Tester, Instron), up to 4 maximum levels of exerted force, namely 5 N, 10 N, 15 N, and 20 N. Real-time pressure mapping was realized by means of sensor matrixes (I-Scan System, Tekscan) applied on the facial surface in four regions (forehead, nasal bridge, zygoma, and maxilla). The data were then transferred on a virtual model created by 3D scans of both the CPAP mask and the phantom head used in the experiments., Results: At increasing applied force, increases in average contact pressure were present at the zygomatic region (1-8 kPa), nasal bridge (12-14 kPa), and forehead (13-29 kPa), while the maxillary region showed relatively stable values (9 kPa). Despite the overall increase in average contact pressure with increasing applied force, no direct proportionality was present. Contact areas did not show clear increments, despite force may redistribute on a larger area, as sensors did not cover the entire mask perimeter. Peak contact pressure values were somehow affected by pressure concentrations that led to saturation in some areas of the sensors (up to 2% of the sensels)., Conclusions: The CPAP mask exerts pressures that may be not uniformly distributed on the face of a subject. This information underlines the clinical importance of assessing both the pressure exerted and the areas that are interested by the mask contact, so as to optimise the CPAP masks design for obtaining a good compromise between ventilation performance and reduction of possible side effects on living tissues., (Copyright © 2022 The Authors. Published by Elsevier Ltd.. All rights reserved.)

Objectives: To evaluate the inhibitory effect of a novel mussel-inspired monomer (N-(3,4-dihydroxyphenethyl)methacrylamide (DMA) on the soluble and matrix-bound proteases., Methods: The inhibitory effect of DMA (0, 1, 5, and 10 mM) and 1 mM chlorhexidine (CHX) dissolved in 50% ethanol/water on soluble recombinant human matrix metalloproteinases (rhMMP-2, -8, and -9), as well as cysteine cathepsins (B and K) were evaluated using both fluorometric assay kits and molecular docking. The effect of CHX and DMA on matrix-bound proteases was examined by in situ zymography, and the fluorescence intensity and relative area were calculated by Image J software. All data obtained were analyzed by one-way ANOVA followed by Tukey test (α = 0.05)., Results: The anti-proteolytic ability of DMA increased in a dose-dependent manner except that of rhMMP-9. Inhibitory effect of 1 mM DMA against rhMMP-2, - 8, - 9, as well as cathepsin B and K was all significantly lower than 1 mM CHX (p < 0.05). The molecular docking analysis was in good agreement with the experimental results, that the binding energy of DMA was lower than CHX for all proteases. In situ zymography revealed that all DMA- and CHX-treated groups significantly inactivated the matrix-bound proteases, with a dramatic reduction of the fluorescence intensity and relative area compared with the control group (p < 0.05)., Significance: Under the prerequisite condition that the overall inhibitory performance on matrix-bound proteases was comparable by DMA and CHX, the more selective property of DMA could avoid inducing potential negative effects by suppressing MMP-9 when applied in dental treatment compared with CHX., Competing Interests: Declaration of Competing Interest The authors declare that they have no conflict of interests., (Copyright © 2022 The Academy of Dental Materials. Published by Elsevier Inc. All rights reserved.)

This study aimed to compare the occlusal morphology and fracture behavior of lithium disilicate ceramic dental crowns on 12 human participants' premolar #45 designed by a knowledge-based AI (CEREC, biogeneric individual function, BI) and different human personnel (experienced technician, TD, and trained dental students, AD) using CAD software. Digital datasets of crown design were best-fit aligned with the original teeth to evaluate profile and volume discrepancies of the occlusal morphology, and difference in the functional cuspal angle. Milled and sintered lithium disilicate crowns were resin-luted to 3D-printed dental casts and were subjected to axial load-to-fracture test. The fracture loads and failure modes were recorded and examined. Repeated measures ANOVA with LSD post-hoc test, Kruskal-Wallis test, Pearson's correlation coefficient, paired t-test, and chi-square exact test were used for statistical analyses (α = 0.05). BI-generated crowns showed the highest occlusal profile discrepancy (0.3677 ± 0.0388 mm), whereas human-CAD designed crowns showed higher conformity to the original teeth (0.3254 ± 0.0515 mm for TD, 0.3571 ± 0.0820 for AD; z-difference method; p < 0.001). Cusp angle values were significantly different in all groups except BI and TD (54.76 ± 3.81° for the original teeth, 70.84 ± 4.31° for BI, 67.45 ± 5.30° for TD, and 62.30 ± 7.92° for AD; p < 0.001). Although all three groups of crown designs could achieve clinically acceptable fracture resistance (1556.09 ± 525.68 N for BI, 1486.00 ± 520.08 N for TD, 1425.77 ± 433.34 for AD; p = 0.505) such that no significant difference in fracture strength was found, most crowns presented catastrophic bulk fracture that was not clinically restorable because of the substrate fracture. Group BI had a significantly higher percentage of restorable substrate damage than TD (p = 0.014) and AD (p < 0.001). In conclusion, in designing lithium disilicate dental crown, CAD design with human may be better than knowledge-based AI., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Objectives: To evaluate the biocompatibility and mechanical properties of experimental bis -phenol-A and bis -GMA free E-glass fiber-reinforced composites (FRCs) prepared with hexanediol dimethacrylate (HDDMA) based resin., Methods: Two ratios of HDDMA/TEGDMA resin were evaluated: exp-1 (70/30 wt.%) and exp-2 (50/50 wt.%) with two bis -GMA resin control groups ( bis -GMA/MMA and bis -GMA/TEGDMA resins, both 70/30 wt.%). E-glass fibers were embedded into the resins to prepare FRCs specimens. Biocompatibility was assessed for cytoviability and biofilm formation with Streptococcus mutans , Streptococcus sanguinis , Enterococcus faecalis, and Candida albicans . Mechanical properties were evaluated for flexural strength and hardness (24 h, water storage 1 and 28 days), water sorption (1, 7, 14, and 28 days), contact angle, and surface roughness. The data were analyzed statistically by one-way and two-way ANOVA (p < 0.05)., Results: Cytoviability of the experimental groups was significantly higher than the control groups (p < 0.05). The exp-1 cytoviability (98.2 ± 1.3%) met the ISO 10993-5 standard requirement for noncytotoxic materials. The adherence of bacteria to the experimental FRCs was visibly less than the controls, while Candida albicans adhered visibly more to the experimental groups than the controls (p < 0.05). Flexural strength showed slightly higher values for controls than for the experimental groups. The exp-1 hardness value was significantly higher in the control groups for all storage conditions (p < 0.05). The water sorption of the experimental groups was significantly higher than the controls. The surface roughness indicated no significant difference (p = 0.87). The exp-1 showed a higher contact angle with the control groups., Conclusion: The experimental HDDMA/TEGDMA-based FRCs might be potential alternatives for bis -GMA-based FRCs., Clinical Significance: The HDDMA/TEGDMA E-glass FRCs might provide biocompatible restorations., Competing Interests: The authors declare no conflict of interest., (© 2022 The Authors.)

Midpalatal suture was analysed with cone beam computed tomography to identify its maturation with respect to age and maxillary-complex growth in 72 patients 9- to 21-year-old. Maxillary-complex was divided in premaxillary, maxillary, and palatine segment. Interdigitation and ossification of midpalatal suture, its density relative to hard tissues and soft tissues, and midpalatal suture density ratio were measured for each segment. Correlation of each parameter with age and maxillary-complex length was analysed, and classification trees were developed for staging parameters. Midpalatal suture maturation stages (MPSMS, from A to E) were applied to assess relationship with age and maxillary-complex length. Regarding age, ossification increased in maxillary segment of males (r S  = 0.39, p = 0.032), while suture density relative to soft tissues increased in maxillary (r S  = 0.37, p = 0.042) and palatine segments (r S  = 0.41, p = 0.027) of males and in palatine segment of females (r S  = 0.32, p = 0.041). In males, suture density relative to soft tissues discriminated two age-stages (p = 0.024), and the same parameter (p = 0.023) paired with ossification (p = 0.027) discriminated two length-stages. MPSMS identified length-differences between stage A and B in females (p = 0.001). Midpalatal-suture ossification and its density relative to soft tissues showed some relationship with age and maxillary-complex length, especially in males. However, challenging staging and limitations in the imaging method may limit clinical applications., (© 2022. The Author(s).)

Objectives: To compare 2 implant scan bodies with different geometry on the accuracy of the virtual alignment process in the digital workflow., Materials and Methods: A master model of the edentulous maxilla with 6 implants and multiunit abutments (MUA) inserted was fabricated. Six dome-shaped and cuboidal scan bodies were mounted on the MUAs, respectively, and consecutively scanned by a laboratory scanner 10 times. The original scans were imported to a dental-specific CAD software and virtually aligned with the default CAD model in the implant library. Thus, 10 aligned models were created. Both the original scans and the aligned models were evaluated by an inspection software for deviation of the scan body surfaces, the centroids of scan body and MUA, the scan body center-axis, and the inter-MUA distances/angles. The two-sample T-test/Mann-Whitney U test were used to analyze the data with the level of significance set at 0.05., Results: The cuboidal group showed significant greater deviations of the model surface (13.9 µm vs. 10.7 µm) and the MUA centroids (31.7 µm vs. 22.8 µm) but smaller deviation of the inter-MUA angle (0.047° vs. 0.070°) than those of the dome-shaped group (p < .05). No significant differences in the deviation of scan body centroids, center-axis, and the inter-MUA distances between the 2 groups were found., Conclusions: Virtual alignment of implant scan body affected the accuracy of the digital workflow for complete-arch implant-supported prostheses (up to ~30 µm/0.09°). Different geometries of the implant scan body could also influence the transfer accuracy in the CAD process., (© 2022 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)

Objectives: To develop a novel auxiliary device for improving the accuracy of intraoral implant scanning in the complete-edentulous arch., Materials and Methods: A standard model of edentulous maxilla with six dummy implants was prepared. Scan bodies were attached to the model, which was scanned by a laboratory scanner. A simulated mucosa (Group 0), a resin base (Group 1), a resin base with a cuboidal reference block and 4 fiduciary spheres (Group 2) or artificial teeth (Group 3) in between the implants were mounted on the model, respectively. Each group were consecutively scanned using an intraoral scanner (n = 10). The scans were analysed for trueness and precision in inter-implant distances and angles by inspection software. Effects of the auxiliary device and different quadrants on the accuracy of complete-arch intraoral scanning were analysed by two-way ANOVA., Results: Significant effects of the auxiliary device and quadrant were found on both linear and angular accuracies. The lowest linear accuracy was found in group 0. Group 1 and group 3 showed the best linear accuracy in quadrant 1 and quadrant 2, respectively. Group 2 showed the least angular precision among the three groups., Conclusions: The three designs of auxiliary devices significantly improved the accuracy of complete-arch intraoral implant scanning. The base-only design showed good scanning accuracy in a single quadrant, while the base with tooth-shaped landmarks design showed better accuracy in cross-arch. The fiduciary spheres might compromise the precision of scanning., (© 2021 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)

Numerous approaches have been developed to improve the resin-dentin bond performance, among which the bio-application of mussel-derived compounds have drawn great attention recently. To assess the performance of N -(3,4-dihydroxyphenethyl)methacrylamide (DMA), a mussel-derived compound, as a functional monomer in dental adhesive, its potential property to cross-link with dentin collagen and polymerize with adhesive will first be evaluated by transmission electron microscopy (TEM), attenuated total reflectance technique of Fourier transform infrared (ATR-FTIR), and atomic force microscopy (AFM) via Peakforce QNM mode. After validating the influence of DMA on collagen and adhesive separately, the overall performance of DMA/ethanol solution as a primer in dentin bonding was examined using micro-tensile bond strength (μTBS) testing, fracture pattern observation, and nanoleakage evaluation both immediately and after 10,000 times thermocycling aging. The inhibitory effect of DMA on endogenous metalloproteinases (MMPs) was evaluated by in situ zymography using confocal laser scanning microscopy (CLSM) and the cytotoxicity of DMA was evaluated using cell counting kit-8. Results demonstrated that DMA successfully cross-linked with dentin collagen via non-covalent bonds and had no influence on the polymerization and mechanical properties of the adhesive. Furthermore, even after 10,000 times thermocycling aging, the μTBS and nanoleakage expression of the DMA-treated groups showed no significant change compared with their immediate values. In situ zymography revealed reduced endogenous proteolytic activities after the application of DMA, and no cytotoxicity effect was observed for DMA concentration up to 25 ​μmol/L. Thus, DMA could be used as a novel, biocompatible functional monomer in dentin bonding., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2021 Published by Elsevier Ltd.)

Statement of Problem: Lithium disilicate (LD) is a popular dental ceramic and has been a focus of dental research. Nevertheless, a quantified literature analysis focusing on the research of lithium disilicate is lacking., Purpose: The purpose of this bibliometric analysis was to review the progress of research into lithium disilicate in dentistry, including the identification of contributing researchers, organizations, countries or regions, journals, and the analysis of high-impact keywords and research trending., Material and Methods: The search was carried out within the topic field of the Web of Science (WoS) database, collecting publications related to LD from between 1996 and 2019. Metadata including the titles, abstracts, keywords, authors, affiliations, countries or regions, and references were extracted. Bibliometric indicators in terms of documents, authors, journals, and keywords were analyzed., Results: Between 1996 and 2019, the dental research on LD trended upward. Scholars in the United States, Germany, Switzerland, and Brazil actively participated in the research on LD, and publications from the United States had the most citations. The Journal of Prosthetic Dentistry and Dental Materials were the major contributing journals. High-impact terms could be categorized into types of restorative material, types of prostheses, and properties or techniques of materials. Co-occurrence and relative normalized citation analysis of keywords highlighted several research interests, such as comparison studies between LD and zirconia, computer-aided design and computer-aided manufacturing (CAD-CAM) techniques, and the performance of complete coverage LD restorations., Conclusions: With the global upsurge in research on lithium disilicate, extensive collaborations and citations have been noted among authors, institutions, and countries or regions. Research hotspots include types of restorative material, types of prostheses, and properties or techniques of materials., (Copyright © 2020 Editorial Council for the Journal of Prosthetic Dentistry. Published by Elsevier Inc. All rights reserved.)

Magnetic resonance (MR) imaging (MRI) provides compelling features for the guidance of interventional procedures, including high-contrast soft tissue imaging, detailed visualization of physiological changes, and thermometry. Laser-based tumor ablation stands to benefit greatly from MRI guidance because 3D resection margins alongside thermal distributions can be evaluated in real time to protect critical structures while ensuring adequate resection margins. However, few studies have investigated the use of projection-based lasers like those for transoral laser microsurgery, potentially because dexterous laser steering is required at the ablation site, raising substantial challenges in the confined MRI bore and its strong magnetic field. Here, we propose an MR-safe soft robotic system for MRI-guided transoral laser microsurgery. Owing to its miniature size (Ø12 × 100 mm), inherent compliance, and five degrees of freedom, the soft robot ensures zero electromagnetic interference with MRI and enables safe and dexterous operation within the confined oral and pharyngeal cavities. The laser manipulator is rapidly fabricated with hybrid soft and hard structures and is powered by microvolume (<0.004 milliter) fluid flow to enable laser steering with enhanced stiffness and lowered hysteresis. A learning-based controller accommodates the inherent nonlinear robot actuation, which was validated with laser path-following tests. Submillimeter laser steering accuracy was demonstrated with a mean error < 0.20 mm. MRI compatibility testing demonstrated zero observable image artifacts during robot operation. Ex vivo tissue ablation and a cadaveric head-and-neck trial were carried out under MRI, where we employed MR thermometry to monitor the tissue ablation margin and thermal diffusion intraoperatively., (Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.)

Objectives: To evaluate the anti-proteolytic and cross-linking effect of N-(3,4-dihydroxyphenethyl)methacrylamide (DMA) on the demineralized dentin matrix in vitro., Methods: Four experimental solutions were selected: 50% ethanol/water solution (Control); 1, 5, and 10 mmol/L DMA dissolved in 50% ethanol/water solution. Sound human molars were sectioned to produce dentin beams with dimension of 1×1×6 mm. The dentin beams were demineralized with 10% phosphoric acid for 8 h to remove the apatite. The demineralized specimens were randomly separated into four groups and immersed in the four experimental solutions for 1 h. After the treatment, the ultimate tensile strength (UTS), loss of dry mass and the release of hydroxyproline by storing the treated specimens in 0.1 mg/mL collagenase solution for 24 h were assessed. The swelling ratio of another ten specimens from each group were evaluated. The interaction between DMA with dentin matrix was observed under Field Emission Scanning Electron Microscopy (FESEM). UTS data was analyzed by two-way ANOVA followed by Tukey test, and the other data was analyzed by one-way ANOVA followed by Tukey test (α = 0.05)., Results: The two-way ANOVA factors, different solutions (p < 0.001), collagenase degradation (p < 0.001) and their interactions (p < 0.001) all significantly affected the UTS. The 10 mM DMA treatment significantly decreased the percentage of loss of dry mass, release of hydroxyproline and swelling ratio of demineralized dentin matrix compared to other treatment groups (p < 0.05). The FESEM observation depicted that with increasing concentration of DMA, the structure of dentin matrix was protected and the porosity within dentin collagen network was decreased., Conclusions: The treatment by 10 mM DMA/ethanol solution for 1 hour is capable of enhancing the mechanical properties of demineralized dentin matrix against collagenase degradation and may be clinically useful to improve the durability of hybrid layer., Clinical Significance: The 10 mM DMA/ethanol primer may offer an alternative choice for dentists to strengthen the mechanical properties of demineralized dentin matrix and resist its degradation by collagenase., (Copyright © 2021. Published by Elsevier Ltd.)

Objectives: The present work describes the status and contents of The Human Bone Collection of the Faculty of Dentistry at the University of Hong Kong., Materials and Methods: The Collection originates from the 1980s and became officially established in 2017 for teaching and research purposes. Most of the Collection consists of unclaimed human remains of southern Chinese individuals exhumed from local cemeteries and donated to the Faculty in the last few decades. The demographic information was provided largely from burial records and forensic estimations. Since 2016, the Collection has undergone a process of reorganization into cranial and postcranial remains, followed by preservation procedures that included cleaning and classification., Results: The Collection currently consists of remains belonging to about 368 individuals (243 males, 54 females, 71 unknown), with ages ranging from 0.8 to 90 years (mean 57.4 years). It comprises cranial remains belonging to 260 individuals (169 males, 39 females, 52 unknown), and postcranial remains belonging to 248 individuals (180 males, 42 females, 26 unknown). The preservation status ranges from poor to good, with the cranial remains better preserved than the postcranial elements. For a large number of individuals, ear ossicles, soil samples, and other materials are also available., Discussion: The Collection is accessible to local and international institutions for teaching and research., (© 2021 Wiley Periodicals LLC.)

Objectives: The present study aimed to evaluate the effect of restoration thickness, CAD/CAM material, and 6 months of artificial saliva storage on the fracture resistance of occlusal veneers., Materials and Methods: A total of 84 intact maxillary molars were sectioned 4.0 mm occlusal to the cementoenamel junction to expose the dentine. The teeth were assigned into 3 main groups according to the type of restorative material (e.max CAD, Vita Enamic, and Lava Ultimate). In each group, the teeth were allocated into 2 subgroups (n = 14) according to restoration thickness (1.0 and 1.5 mm). The veneers were adhesively bonded using dual-cure self-adhesive luting agent. A total of 42 specimens comprising half the tested subgroups were stored in distilled water for 24-h before the test. The remaining half was stored in artificial saliva at 37 ± 1 °C in an incubator for 6 months. All specimens (n = 84) were subjected to 5000 thermal cycles between 5 and 55 °C ± 2 before the fracture resistance test. The maximum force at fracture was recorded in Newton. Failure mode was analyzed using a stereomicroscope. The results were analyzed using a parametric Three-way ANOVA test., Results: The results of the Three-way ANOVA test revealed that material type and restoration thickness significantly affected fracture resistance values (p < 0.5), while 6 months of storage in artificial saliva had no significant effect on mean fracture resistance values (p˃0.5). The most common failure patterns in CAD/CAM resin composite and polymer-infiltrated ceramics were scores I and score II. For glass ceramic groups, score IV and III were more dominant., Conclusions: All the tested CAD/CAM restorations in both thicknesses exhibited fracture resistance values exceeding normal and parafunctional bite forces. Polymer-infiltrated ceramics and CAD/CAM resin composite veneers showed more favorable fracture patterns., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Objective: To give a current review of the mechanism of mussel adhesion, the application of mussel-inspired compounds in dentistry and the challenges associated with clinical application., Methods: Inspired by the wet adhesion property of 3,4-dihydroxyphenol-l-alanine (Dopa) in mussel plaques, various chemical compounds have been synthesized to mimic the mussel as an adhesion model for medical applications. Similar to mussels in the marine environment, dental materials in the oral environment have to endure long-term water hydrolysis, mechanical stress and other chemical challenges. These challenges have influenced an increasing number of studies that are exploring the translation of mussel-inspired adhesion to clinical applications. Therefore, this review discusses the mussel adhesion chemistry and its related application in dentistry., Results: Mussel-inspired compounds have achieved relatively acceptable performances in various dental fields, including surface coating, metal ions chelation, dentin bonding and mucosal adhesion. However, two practical problems remain to be comprehensively addressed, namely the protection of catechol groups from oxidation, and the feasibility for clinical application., Significance: The mussel's wet adhesion ability has attracted much research interest in the dental field because of its properties of moisture-resistant adhesion and surface coating. Despite the emergence of several mussel-inspired compounds in recent years, a comprehensive and timely review of their applications in dentistry is lacking. Therefore, the current review hopes to provide valuable information around the application of mussel-inspired compounds in dentistry with their pros and cons discussed., (Copyright © 2021 The Academy of Dental Materials. Published by Elsevier Inc. All rights reserved.)

Background: The fit of implant-supported prostheses is of prime importance for the long-term success of implant therapy., Purpose: This systematic review aimed to evaluate recent evidence on current techniques for assessing implant-framework misfit, its associated strain/stress, and whether these misfits are related to mechanical, biological, and clinical consequences., Materials and Methods: An electronic search for publications from January 2010 to October 2020 was performed using the Pubmed, Embase, Web of Science, and Cochrane Library databases with combined keywords on implant-framework misfit assessments and related clinical complications. Inclusion and exclusion criteria were applied. After full-text analyses, data extraction was implemented on current techniques of misfit assessment and the relationship between the misfit and the induced strain/stress., Results: A total of 3 in vivo and 92 in vitro studies were selected, including 47 studies on quantifying the degree of implant-framework misfit with dimensional techniques, 24 studies measuring misfit-induced strain/stress with modeling techniques, and 24 studies using both methods. The technical details, advantages, and limitations of each technique were illustrated. The correlation between the implant-framework misfit and the induced strain/stress has been revealed in vitro, while that with the biological complications and implant/prostheses failure was weak in clinical studies., Conclusions: Dimensional and modeling techniques are available to measure the implant-framework misfit. The passivity of implant-supported fixed prostheses appeared related to the induced strain/stress, but not the clinical complications. Further studies combining three-dimensional (3D) assessments using dimensional and modeling techniques was needed., (© 2020 Wiley Periodicals LLC.)

Background: Monolithic restorations made of translucent yttria-stabilized tetragonal zirconia polycrystal (Y-TZP) have become popular over the past few decades. However, whether aging affects the color and translucency of monolithic translucent Y-TZP is unclear., Objective: The aim of this systematic review and meta-analysis of in vitro studies was to evaluate the effects of aging on the color and translucency of monolithic translucent Y-TZP ceramics., Materials and Methods: This systematic review/meta-analysis was reported according to the PRISMA statement and registered in the OSF registries (https://osf.io/5qjmu). Four databases including Medline via the PubMed, Embase, and Web of Science databases and the Cochrane Library were searched using no publication year and language limits. The last search was executed on November 20, 2020. In vitro studies comparing the translucency and/or color of monolithic translucent Y-TZP ceramics before and after simulated aging were selected. Meta-analyses were performed using Review Manager software (version 5.3, Cochrane Collaboration, Oxford, UK) with random-effects models at a significance level of 0.05. A risk-of-bias assessment was also performed for the included studies., Results: Of the 188 potentially relevant studies, 13 were included in the systematic review. The hydrothermal aging duration ranged from 1 to 100 h at relatively similar temperatures (~134°C). In the general meta-analyses, the aged Y-TZP ceramics exhibited similar translucency parameter (TP), L ∗ , and b ∗ values compared with the nonaged controls ( P = .73, P = .49, and P = .62, respectively). Moreover, there was a significant difference between the aged and nonaged Y-TZP ceramics in the a ∗ value ( P = .03; MD = -0.26; 95% CI = -0.51 to - 0.02), favoring the nonaged Y-TZP ceramics. The subgroup analyses showed that the duration of aging contributed to changes in the translucency and color of the Y-TZP ceramics., Conclusions: The optical properties of monolithic translucent Y-TZP ceramics were stable after hydrothermal aging at 134°C and 0.2 MPa for ≤20 h. Moreover, clinically unacceptable changes in the translucency and color of monolithic translucent Y-TZP ceramics were found after hydrothermal aging for >20 h., Competing Interests: The authors declare that they have no conflicts of interest., (Copyright © 2021 Chang-yuan Zhang et al.)

Learning bone anatomy of the skull is a complex topic involving three-dimensional information. The impact of the use of human dry skulls and cone beam computed tomography (CBCT) imaging was investigated in the teaching of undergraduate dental students. Sixty-four first-year students in the University of Hong Kong were randomly divided into eight groups. Four teaching methods were tested: (1) CBCT followed by standard lecture, (2) CBCT followed by lecture with skulls, (3) standard lecture followed by CBCT, and (4) lecture with skulls followed by CBCT. After each, students were given a multiple-choice questionnaire to assess their objective learning outcome (20 questions) and a questionnaire for their subjective satisfaction (10 statements). Surveys were assessed with Cronbach's alpha, Kendall's tau-b, and principal components analysis. Data were analyzed with Student's t-test and a one-way ANOVA (significance α = 0.05). Standard lecture followed by CBCT showed the highest learning outcome score (81.6% ± 14.1%), but no significant difference was present among four teaching methods. Cone beam computed tomography followed by lecture with skulls scored the highest overall subjective satisfaction (4.9 ± 0.8 out of 6), but no significant difference was present among teaching methods. Nevertheless, students' perception of learning was positively influenced by the use of skulls (P = 0.018). The timing of administration of the CBCT did not affect students' subjective satisfaction or objective learning outcome. Students perceived to learn more by using skulls, but their objective learning outcomes were not significantly affected. A discrepancy seems to exist between students' perception of learning and their effective performance., (© 2020 American Association for Anatomy.)