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Quantitative electroencephalographic activity over the left and right frontal, temporal, parietal, and occipital lobes was obtained on 4 successive weeks in 12 subjects before they were exposed cerebrally for 30 min to one of two configurations of weak complex magnetic fields or to a sham-field condition. The two configurations were Shakti (c Todd Murphy) and the set of 4 solenoids (Koren boxes) generating a burst-firing magnetic field through the temporal lobes. Compared to baseline measurements there were no statistically significant differences in treatments for relative changes in power over these regions within the delta, theta, low alpha, beta, or gamma ranges. However within the high alpha range (10.5 Hz to 13 Hz), there was a significant interaction between session and type of treatment that was due primarily to the Shakti treatment. These changes were congruent with the subjective experiences reported by some users of the technology. [ABSTRACT FROM AUTHOR]

Competing Interests: All authors have disclosed no conflicts of interest.

Sequences of three Arabidopsis thaliana and two Brassica napus cDNAs encoding squalene monooxygenase homologues (Sqp1 and Sqp2) are reported. Southern analysis confirmed that these cDNAs are derived from small gene families in both species. Expression analysis indicates that Sqp1 genes in B. napus are strongly expressed in leaves but not roots or developing seeds. Comparison of cDNA and genomic sequences indicate that the 3' splice site of an intron in these genes has undergone junctional sliding. The evolutionary significance of this phenomenon is discussed.

The Biolistic(®) microprojectile DNA-delivery method was used to test the usefulness in conifers of eight gene constructs based on the 35S promoter, the AMV translational enhancer, and gene fusion between the P-glucuronidase and the neomycin phosphotransferase II genes. The evaluation was done with embryogenic cells of Picea glauca, where the relative strengths of the promoters were 35S-35S-AMVE>35S-AMVE>35S-35S>35S as evaluated by transient gene expression. The fusion gene of GUS and NPT II gave lower levels of transient gene expression than the unfused GUS gene as detected by X-GLU histochemical assays. Experiments comparing the EM promoter of wheat and the 35S-35S-AMVE promoter (with and without fusion between GUS and NPT II) were done in Picea rubens, P. mariana, P. glauca, and Larix x eurolepis. The unfused gene with the 35S-35S-AMVE promoter gave higher levels of transient gene expression than the fused GUS-NPT II gene. The fluorescent MUG assay was more sensitive than the histochemical X-GLU assay to detect the activity of the β-glucuronidase gene.