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41 results on '"Tsagogiorgas, Charalambos"'

14. Intravenous delivery of granulocyte-macrophage colony stimulating factor impairs survival in lipopolysaccharide-induced sepsis

Author

Krebs, Jörg, Hillenbrand, Alexander, Tsagogiorgas, Charalambos, Patry, Christian, Tönshoff, Burkhard, Yard, Benito, Beck, Grietje, and Rafat, Neysan

Subjects
Lipopolysaccharides, Male, Physiology, Death Rates, Science, Immunology, Toxic Agents, Bacterial Toxins, Pathology and Laboratory Medicine, Research and Analysis Methods, Toxicology, Monocytes, Signs and Symptoms, Population Metrics, Diagnostic Medicine, Animal Cells, Sepsis, Immune Physiology, Medicine and Health Sciences, Toxins, Animals, Enzyme-Linked Immunoassays, Rats, Wistar, Immunoassays, Cells, Cultured, Innate Immune System, Population Biology, Stem Cells, Biology and Life Sciences, Granulocyte-Macrophage Colony-Stimulating Factor, Cell Biology, Molecular Development, Body Fluids, Rats, Endotoxins, Blood, Immune System, Immunologic Techniques, Medicine, Cytokines, Cellular Types, Anatomy, Spleen, Research Article, Developmental Biology
Abstract

BackgroundCell-based therapies with bone marrow-derived progenitor cells (BMDPC) lead to an improved clinical outcome in animal sepsis models. In the present study we evaluated the ability of granulocyte macrophage-colony stimulating factor (GM-CSF) to mobilize BMDPC in a lipopolysaccharide (LPS)-induced sepsis model and thereby its potential as a novel treatment strategy.MethodsMale Wistar rats received LPS (25μg/kg/h for 4 days) intravenously and were subsequently treated with GM-CSF 12.5μg/kg (0h,24h,48h,72h). As control groups, rats were infused with sodium chloride or GM-CSF only. Clinical and laboratory parameters, proinflammatory plasma cytokines as well as BMDPC counts were analyzed. Cytokine release by isolated peripheral blood mononuclear cells from rat spleen upon incubation with LPS, GM-CSF and a combination of both were investigated in vitro.ResultsIn vivo, rats receiving both LPS and GM-CSF, showed a reduced weight loss and increased mobilization of BMDPC. At the same time, this regime resulted in an increased release of proinflammatory cytokines (IL-6, IL-8) and a significantly increased mortality. In vitro, the combination of LPS and GM-CSF showed a significantly increased IL-6 release upon incubation compared to incubation with LPS or GM-CSF alone.ConclusionsGM-CSF did not have a beneficial effect on the clinical course in our LPS-induced sepsis model. It synergistically promoted inflammation with LPS and probably thereby impaired survival.

Published
2019

15. Impact of different emulsifiers on biocompatibility and inflammatory potential of Perfluorohexyloctane (F6H8) emulsions for new intravenous drug delivery systems

Author

Tsagogiorgas,Charalambos, Anger,Friedrich, Beck,Grietje, Breedijk,Annette, Yard,Benito, Hoeger,Simone, Tsagogiorgas,Charalambos, Anger,Friedrich, Beck,Grietje, Breedijk,Annette, Yard,Benito, and Hoeger,Simone

Abstract

Charalambos Tsagogiorgas,1 Friedrich Anger,1,2 Grietje Beck,1 Annette Breedijk,3 Benito Yard,3 Simone Hoeger31Department of Anaesthesiology and Critical Care Medicine, University Hospital Mannheim, Faculty of Medicine, University of Heidelberg, Mannheim, Germany; 2Department of General, Visceral, Vascular and Paediatric Surgery, Julius-Maximilians-Universität, University of Wuerzburg, Wurzburg, Germany; 3Department of Internal Medicine V, University Hospital Mannheim, Faculty of Medicine, University of Heidelberg, Mannheim, GermanyBackground: Emulsions on the basis of Perfluorohexyloctane (F6H8), a semifluorinated alkane (SFA), have shown to dissolve and transport highly lipophilic compounds. It is unknown how F6H8-containing emulsions (F6H8-cEM) interact with compartment blood, the reticuloendothelial system (RES), or influence injured organs in vivo. The current study was conducted to investigate the in vitro biocompatibility of F6H8-cEM and their drug delivery properties. Afterward, an in vivo study was performed as a proof-of-concept study in a rat model of acute kidney injury (AKI), which focused on the potential influence of F6H8-cEM on inflammation in an injured organ.Methods: Two different F6H8-cEM were stabilized by the emulsifying agents Poloxamer 188 (Pluronic® F68) or lecithin (S75). The two resulting emulsions F6H8-Pluronic or F6H8-lecithin were tested in vitro for the potential modulation of acute inflammation via whole blood assay, FACS, and ELISA. Antioxidant capacity and drug delivery properties were measured with an oxidation assay. Secondly, AKI was induced in the rats, which were treated with the F6H8-lecithin emulsion. Renal function and inflammation were assessed.Results: Both F6H8-cEM were phagocytized by monocytes and both dose-dependently affected apoptosis (Annexin V binding) in monocytes. TNF-α expression increased dose-dependency for F6H8-Pluronic emulsion but not for F6H8-lecithin in a whole blood assa

Published
2019

19. Computed Tomography–Assisted Thoracoscopic Surgery

Author

Kostrzewa, Michael, primary, Kara, Kerim, additional, Rathmann, Nils, additional, Tsagogiorgas, Charalambos, additional, Henzler, Thomas, additional, Schoenberg, Stefan O., additional, Hohenberger, Peter, additional, Diehl, Steffen J., additional, and Roessner, Eric D., additional

Published
2017
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21. 19F Oximetry with semifluorinated alkanes

Author

Kegel, Stefan, primary, Chacon-Caldera, Jorge, additional, Tsagogiorgas, Charalambos, additional, Theisinger, Bastian, additional, Glatting, Gerhard, additional, and Schad, Lothar R., additional

Published
2015
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22. N-octanoyl dopamine treatment exerts renoprotective properties in acute kidney injury but not in renal allograft recipients

Author

Klotz, Sarah, primary, Pallavi, Prama, additional, Tsagogiorgas, Charalambos, additional, Zimmer, Fabian, additional, Zöllner, Frank G., additional, Binzen, Uta, additional, Greffrath, Wolfgang, additional, Treede, Rolf-Detlef, additional, Walter, Jakob, additional, Harmsen, Martin C., additional, Krämer, Bernhard K., additional, Hafner, Mathias, additional, Yard, Benito A., additional, and Hoeger, Simone, additional

Published
2015
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24. F Oximetry with semifluorinated alkanes.

Author

Kegel, Stefan, Chacon-Caldera, Jorge, Tsagogiorgas, Charalambos, Theisinger, Bastian, Glatting, Gerhard, and Schad, Lothar R.

Subjects
OXIMETRY, ALKANES, PERFLUOROCARBONS, HYDROGEN-ion concentration, IN vitro studies
Abstract

This work examines the variation of longitudinal relaxation rate R1(= 1/T1) of the19F-CF3-resonance of semifluorinated alkanes (SFAs) with oxygen tension (pO2), temperature (T) and pHin vitro. Contrary to their related perfluorocarbons (PFCs), SFA are amphiphilic and facilitate stable emulsions, a prerequisite for clinical use. A linear relationship between R1 and pO2was confirmed for the observed SFAs at different temperatures. Using a standard saturation recovery sequence, T1 has been successfully measured using fluorine19F-MRI with a self-constructed birdcage resonator at 9.4 T. A calibration curve to calculate pO2depending on T and R1 was found for each SFA used. In contrast to the commonly used PFC, SFAs are less sensitive to changes in pO2, but more sensitive to changes in temperature. The influence of pH to R1 was found to be negligible. [ABSTRACT FROM AUTHOR]

Published
2016
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27. Enhancing tissue repair in annulus fibrosus defects of the intervertebral disc: analysis of a bio‐integrative annulus implant in an in‐vivo ovine model

Author

Hegewald, Aldemar Andres, primary, Medved, Fabian, additional, Feng, Daxiong, additional, Tsagogiorgas, Charalambos, additional, Beierfuß, Anja, additional, Schindler, Genevieve Ama Kyremaa, additional, Trunk, Marcus, additional, Kaps, Christian, additional, Mern, Demissew Shenegelegn, additional, and Thomé, Claudius, additional

Published
2013
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29. N-Octanoyl Dopamine Inhibits the Expression of a Subset of κB Regulated Genes: Potential Role of p65 Ser276 Phosphorylation

Author

Hottenrott, Maximilia C., primary, Wedel, Johannes, additional, Gaertner, Sophie, additional, Stamellou, Eleni, additional, Kraaij, Tineke, additional, Mandel, Linda, additional, Loesel, Ralf, additional, Sticht, Carsten, additional, Hoeger, Simone, additional, Ait-Hsiko, Lamia, additional, Schedel, Angelika, additional, Hafner, Mathias, additional, Yard, Benito, additional, and Tsagogiorgas, Charalambos, additional

Published
2013
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31. Correction: N-octanoyl-Dopamine Is an Agonist at the Capsaicin Receptor TRPV1 and Mitigates Is Chemia-Induced Acute Kidney Injury in Rat

Author

Tsagogiorgas, Charalambos, primary, Wedel, Johannes, additional, Hottenrott, Maximilia, additional, Schneider, Michael O., additional, Binzen, Uta, additional, Greffrath, Wolfgang, additional, Treede, Rolf-Detlef, additional, Theisinger, Bastian, additional, Theisinger, Sonja, additional, Waldherr, Rüdiger, additional, Krämer, Bernhard K., additional, Thiel, Manfred, additional, Schnuelle, Peter, additional, Yard, Benito A., additional, and Hoeger, Simone, additional

Published
2012
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32. N-octanoyl-Dopamine Is an Agonist at the Capsaicin Receptor TRPV1 and Mitigates Is Chemia-Induced Acute Kidney Injury in Rat

Author

Tsagogiorgas, Charalambos, primary, Wedel, Johannes, additional, Hottenrott, Maximilia, additional, Schneider, Michael O., additional, Binzen, Uta, additional, Greffrath, Wolfgang, additional, Treede, Rolf-Detlef, additional, Theisinger, Bastian, additional, Theisinger, Sonja, additional, Waldherr, Rüdiger, additional, Krämer, Bernhard K., additional, Thiel, Manfred, additional, Schnuelle, Peter, additional, Yard, Benito A., additional, and Hoeger, Simone, additional

Published
2012
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33. UW is Superior Compared with HTK After Prolonged Preservation of Renal Grafts

Author

Hoeger, Simone, primary, Lueg, Georgia, additional, Tsagogiorgas, Charalambos, additional, Schneider, Michael, additional, Theisinger, Sonja, additional, Theisinger, Bastian, additional, Fontana, Johann, additional, Waldherr, Ruediger, additional, Krämer, Bernhard K., additional, Schnuelle, Peter, additional, and Yard, Benito, additional

Published
2011
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39. 19 F Oximetry with semifluorinated alkanes.

Author

Kegel S, Chacon-Caldera J, Tsagogiorgas C, Theisinger B, Glatting G, and Schad LR

Subjects
Animals, Humans, Fluorine-19 Magnetic Resonance Imaging methods, Fluorocarbons chemistry, Oximetry methods
Abstract

This work examines the variation of longitudinal relaxation rate R1(= 1/T1) of the 19 F-CF 3 -resonance of semifluorinated alkanes (SFAs) with oxygen tension (pO 2 ), temperature (T) and pH in vitro. Contrary to their related perfluorocarbons (PFCs), SFA are amphiphilic and facilitate stable emulsions, a prerequisite for clinical use. A linear relationship between R1 and pO 2 was confirmed for the observed SFAs at different temperatures. Using a standard saturation recovery sequence, T1 has been successfully measured using fluorine 19 F-MRI with a self-constructed birdcage resonator at 9.4 T. A calibration curve to calculate pO 2 depending on T and R1 was found for each SFA used. In contrast to the commonly used PFC, SFAs are less sensitive to changes in pO 2 , but more sensitive to changes in temperature. The influence of pH to R1 was found to be negligible.

Published
2016
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40. Dopamine and lipophilic derivates protect cardiomyocytes against cold preservation injury.

Author

Vettel C, Hottenrott MC, Spindler R, Benck U, Schnuelle P, Tsagogiorgas C, Krämer BK, Hoeger S, El-Armouche A, Wieland T, and Yard BA

Subjects
Animals, Cells, Cultured, Cold Temperature adverse effects, Female, Male, Myocytes, Cardiac pathology, Organ Preservation methods, Rats, Rats, Inbred Lew, Rats, Wistar, Cardiotonic Agents pharmacology, Cryopreservation methods, Dopamine analogs & derivatives, Dopamine pharmacology, Myocytes, Cardiac drug effects, Organ Preservation adverse effects
Abstract

Donor heart allografts are extremely susceptible to prolonged static cold storage. Because donor treatment with low-dose dopamine improves clinical outcome after heart transplantation, we tested the hypothesis that dopamine and its lipophilic derivate, N-octanoyl dopamine (NOD), protect cardiomyocytes from cold storage injury. Neonatal rat cardiomyocytes were treated with dopamine or NOD or left untreated and subsequently subjected to static cold storage (8-12 hours). Dopamine- and NOD-treated cardiomyocytes displayed a better viability compared with untreated cells after hypothermia. In untreated cardiomyocytes, cell damage was reflected by lactate dehydrogenase (LDH) release and a decrease in intracellular ATP. NOD was approximately 20-fold more potent than dopamine. Similarly to cardiomyocytes in vitro, rat hearts perfused with NOD before explantation showed significantly lower LDH release after static cold storage. ATP regeneration and spontaneous contractions after cold storage and rewarming only occurred in treated cardiomyocytes. Hypothermia severely attenuated isoprenaline-induced cAMP formation in control but not in dopamine- or NOD-treated cells. Esterified derivates of NOD with redox potential and lipophilic side chains reduced cell damage during cold storage similarly to NOD. In contrast to dopamine, neither NOD nor its derivates induced a significant β-adrenoceptor-mediated elevation of cellular cAMP levels. The β1-adrenoceptor antagonist atenolol and D1/D2 receptor antagonist fluphenazine had no impact on the protective effect of NOD or dopamine. We conclude that dopamine as well as NOD treatment mitigates cold preservation injury to cardiomyocytes. The beneficial effects are independent of β-adrenoceptor or dopaminergic receptor stimulation but correlate with redox potential and lipophilic properties.

Published
2014
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41. N-octanoyl-dopamine is an agonist at the capsaicin receptor TRPV1 and mitigates ischemia-induced [corrected] acute kidney injury in rat.

Author

Tsagogiorgas C, Wedel J, Hottenrott M, Schneider MO, Binzen U, Greffrath W, Treede RD, Theisinger B, Theisinger S, Waldherr R, Krämer BK, Thiel M, Schnuelle P, Yard BA, and Hoeger S

Subjects
Animals, Blotting, Western, Electrophoretic Mobility Shift Assay, Ganglia, Spinal cytology, Ganglia, Spinal metabolism, Immunohistochemistry, Male, NF-kappa B metabolism, Neurons drug effects, Neurons metabolism, Peripheral Nerves drug effects, Peripheral Nerves metabolism, Polymerase Chain Reaction, Rats, Vascular Cell Adhesion Molecule-1 metabolism, Acute Kidney Injury drug therapy, Dopamine analogs & derivatives, Dopamine therapeutic use, TRPV Cation Channels agonists
Abstract

Since stimulation of transient receptor potential channels of the vanilloid receptor subtype 1 (TRPV1) mitigates acute kidney injury (AKI) and endogenous N-acyl dopamine derivatives are able to activate TRPV1, we tested if synthetic N-octanoyl-dopamine (NOD) activates TRPV1 and if it improves AKI. These properties of NOD and its intrinsic anti-inflammatory character were compared with those of dopamine (DA). TRPV1 activation and anti-inflammatory properties of NOD and DA were tested using primary cell cultures in vitro. The influence of NOD and DA on AKI was tested in a prospective, randomized, controlled animal study with 42 inbred male Lewis rats (LEW, RT1), treated intravenously with equimolar concentrations of DA or NOD one hour before the onset of warm ischemia and immediately before clamp release. NOD, but not DA, activates TRPV1 channels in isolated dorsal root ganglion neurons (DRG) that innervate several tissues including kidney. In TNFα stimulated proximal tubular epithelial cells, inhibition of NFκB and subsequent inhibition of VCAM1 expression by NOD was significantly stronger than by DA. NOD improved renal function compared to DA and saline controls. Histology revealed protective effects of NOD on tubular epithelium at day 5 and a reduced number of monocytes in renal tissue of DA and NOD treated rats. Our data demonstrate that NOD but not DA activates TRPV1 and that NOD has superior anti-inflammatory properties in vitro. Although NOD mitigates deterioration in renal function after AKI, further studies are required to assess to what extend this is causally related to TRPV1 activation and/or desensitization.

Published
2012
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