1. Multiplexed mass spectrometry of individual ions improves measurement of proteoforms and their complexes
- Author
-
Deven L. Shinholt, Joshua T. Maze, Ryan T. Fellers, Bon Ikwuagwu, Steven C. Beu, Neil L. Kelleher, Kenneth R. Durbin, Philip D. Compton, Ping Yip, Rafael D. Melani, Danielle Tullman-Ercek, Bryan P. Early, Alexander Makarov, Michael W. Senko, Jared O. Kafader, and Vlad Zabrouskov
- Subjects
Proteomics ,Analytical chemistry ,Mass spectrometry ,Orbitrap ,Biochemistry ,Multiplexing ,Article ,Mass Spectrometry ,Spectral line ,Charge detection ,Ion ,law.invention ,03 medical and health sciences ,Physics::Plasma Physics ,law ,Humans ,Molecular Biology ,030304 developmental biology ,Quantitative Biology::Biomolecules ,0303 health sciences ,Chemistry ,Proteins ,Cell Biology ,Characterization (materials science) ,True mass ,Biotechnology - Abstract
A new Orbitrap-based ion analysis procedure is shown to be possible by determining the direct charge for numerous individual protein ions to generate true mass spectra. The deployment of an Orbitrap system for charge detection enables the characterization of highly complicated mixtures of proteoforms and their complexes in both denatured and native modes of operation, revealing information not obtainable by traditional measurement of an ensemble of ions.
- Published
- 2020
- Full Text
- View/download PDF