25 results on '"Trucco D"'
Search Results
2. LOW-INTENSITY PULSED ULTRASOUND INDUCES CHONDROGENIC DIFFERENTIATION OF ADIPOSE-STROMAL CELLS IN 3D PIEZOELECTRIC HYDROGEL
- Author
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Manferdini, C., primary, Gabusi, E., additional, Dolzani, P., additional, Saleh, Y., additional, Trucco, D., additional, Columbaro, M., additional, Vannozzi, L., additional, Cafarelli, A., additional, Ricotti, L., additional, and Lisignoli, G., additional
- Published
- 2022
- Full Text
- View/download PDF
3. Solidarités et (dé)politisation de la frontière
- Author
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Giliberti, L., Potot, S., and Trucco, D.
- Published
- 2020
4. Specific effects of osteoarthritic milieu and hypoxic conditions on adipose mesenchymal stromal cell migration and cytokine receptors expression
- Author
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Manferdini, C., primary, Gabusi, E., additional, Trucco, D., additional, Rojewski, M., additional, Schrezenmeier, H., additional, Meliconi, R., additional, Addimanda, O., additional, and Lisignoli, G., additional
- Published
- 2020
- Full Text
- View/download PDF
5. Adipose-derived mesenchymal stromal cells specifically modulated CXCL10/IP10 chemokine detected in osteoarthritic milieu
- Author
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Manferdini, C., primary, Gabusi, E., additional, Trucco, D., additional, Saleh, Y., additional, and Lisignoli, G., additional
- Published
- 2020
- Full Text
- View/download PDF
6. Osteoarthritic milieu and hypoxia exert specific effects on adipose mesenchymal stromal cell migration and cytokine receptor expression
- Author
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Manferdini, C., primary, Paolella, F., additional, Gabusi, E., additional, Trucco, D., additional, Cattini, L., additional, Rojewski, M., additional, Schrezenmeier, H., additional, Addimanda, O., additional, Meliconi, R., additional, and Lisignoli, G., additional
- Published
- 2019
- Full Text
- View/download PDF
7. The two-way paradigm of transplantation immunology
- Author
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Rao, AS, Starzl, TE, Demetris, AJ, Trucco, D, Thomson, A, Qian, SG, Murase, N, Fung, JJ, Rao, AS, Starzl, TE, Demetris, AJ, Trucco, D, Thomson, A, Qian, SG, Murase, N, and Fung, JJ
- Published
- 1996
8. [Sepsis in children with malignant neoplasia, equipped with a Broviac-type venous catheter]
- Author
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Garaventa A, Castagnola E, Sandro Dallorso, Dini G, Trucco D, Vianello O, Carrega G, Cuneo P, Buffa P, and Magillo P
- Subjects
Male ,Catheterization, Central Venous ,Parenteral Nutrition ,Neutropenia ,Antineoplastic Agents ,Gram-Positive Bacteria ,Catheters, Indwelling ,Neoplasms ,Sepsis ,Gram-Negative Bacteria ,Humans ,Female ,Child ,Infusions, Intravenous - Abstract
Indwelling central venous catheters obviate many problems in the care of children with malignancies, but they also are a well-known source of infection. We are reviewed the history of 584 Broviac catheters inserted from January 1984 to December 1991, in 475 children with cancer in order to assess the etiology of bacteremias, their association with neutropenia and their relationship with the presence of the catheters. The overall duration-time of the catheters, employed for blood tests, drug and blood infusions and parenteral nutrition, was 1-835 days (median 263, mean 186). Total catheter courses was 108.678 days. In this period 226 episodes of sepsis were observed in 180 patients: 157 in neutropenic patients and 69 in non neutropenic. Catheter related bacteremias were diagnosed in 65/226 episodes (29%): 23 (35%) were observed in neutropenic patients and 42 (65%) in non neutropenic (P0.005). Gram-positive pathogens were isolated in 28/65 (43%) episodes, Gram-negatives in 15/65 (23%), fungi in 9/65 (14%), and the remaining 13 (20%) were polymicrobial. In the last years we observed an increase of catheter related bacteremias due to Gram-negative rods no change was observed in pathogens causing catheters unrelated bacteremias. The high incidence of catheters related bacteremias in non neutropenic, non hospitalized patients, stress on the home-care of the catheters; a high level of suspicion of Gram-negative infections should be maintained in cancer patients with an indwelling central venous catheters.
9. Home care program in a paediatric haematology and oncology department. Results of the first year of activity,Assistenza domiciliare in emato-oncologia pediatrica. Risultati del primo anno di attività
- Author
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Miano, M., Manfredini, L., Garaventa, A., Riccardo Haupt, Fieramosca, S., Tanasini, R., Leimer, M., Trucco, D., Rossi, R., and Dini, G.
10. Feasibility of a home care program in a pediatric hematology and oncology department. Results of the first year of activity at a single institution
- Author
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Maurizio Miano, Manfredini L, Garaventa A, Fieramosca S, Tanasini R, Leimer M, Trucco D, Rossi R, Haupt R, and Dini G
11. Proposte di linee guida per un'attività integratatra infermieri e medici di ematologia ed oncologia pediatrica: Gruppo di lavoro integrato medici-infermieri dell'Associazione Italiana di Ematologia ed Oncologia Pediatrica
- Author
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Ricciardi, C., Bellin, S., Borazio, M., Donfrancesco, A., Ferrero, C., Garaventa, A., Jankovic, M., La Dogana, S., Mazzanti, E., Elena Rostagno, Steduto, M., Tobanelli, P., Trucco, D., Zampier, C., and Perilongo, G.
12. Wear Behavior Characterization of Hydrogels Constructs for Cartilage Tissue Replacement
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Paola Taddei, Lorenzo Vannozzi, Diego Trucco, Saverio Affatato, Gina Lisignoli, Gilbert Daniel Nessim, Leonardo Ricotti, Affatato S., Trucco D., Taddei P., Vannozzi L., Ricotti L., Nessim G.D., and Lisignoli G.
- Subjects
Materials science ,Tissue replacement ,Oxide ,knee simulator ,Articular cartilage ,02 engineering and technology ,knee arthroplasty ,micro-CT ,010402 general chemistry ,01 natural sciences ,lcsh:Technology ,Article ,Hydrogel ,Knee arthroplasty ,Knee simulator ,Micro-CT ,Raman spectroscopy ,Roughness measurements ,chemistry.chemical_compound ,symbols.namesake ,roughness measurements ,medicine ,General Materials Science ,articular cartilage ,lcsh:Microscopy ,lcsh:QC120-168.85 ,lcsh:QH201-278.5 ,lcsh:T ,Cartilage ,technology, industry, and agriculture ,021001 nanoscience & nanotechnology ,Gellan gum ,0104 chemical sciences ,3. Good health ,medicine.anatomical_structure ,Chemical engineering ,chemistry ,lcsh:TA1-2040 ,Self-healing hydrogels ,symbols ,lcsh:Descriptive and experimental mechanics ,lcsh:Electrical engineering. Electronics. Nuclear engineering ,hydrogel ,0210 nano-technology ,lcsh:Engineering (General). Civil engineering (General) ,Ethylene glycol ,lcsh:TK1-9971 - Abstract
This paper aims to characterize the wear behavior of hydrogel constructs designed for human articular cartilage replacement. To this purpose, poly (ethylene glycol) diacrylate (PEGDA) 10% w/v and gellan gum (GG) 1.5% w/v were used to reproduce the superior (SUP) cartilage layer and PEGDA 15% w/v and GG 1.5% w/v were used to reproduce the deep (DEEP) cartilage layer, with or without graphene oxide (GO). These materials (SUP and DEEP) were analyzed alone and in combination to mimic the zonal architecture of human articular cartilage. The developed constructs were tested using a four-station displacement control knee joint simulator under bovine calf serum. Roughness and micro-computer tomography (µ, CT) measurements evidenced that the hydrogels with 10% w/v of PEGDA showed a worse behavior both in terms of roughness increase and loss of uniformly distributed density than 15% w/v of PEGDA. The simultaneous presence of GO and 15% w/v PEGDA contributed to keeping the hydrogel construct&rsquo, s characteristics. The Raman spectra of the control samples showed the presence of unreacted C=C bonds in all the hydrogels. The degree of crosslinking increased along the series SUP <, DEEP + SUP <, DEEP without GO. The Raman spectra of the tested hydrogels showed the loss of diacrylate groups in all the samples, due to the washout of unreacted PEGDA in bovine calf serum aqueous environment. The loss decreased along the series SUP >, DEEP + SUP >, DEEP, further confirming that the degree of photo-crosslinking of the starting materials plays a key role in determining their wear behavior. &mu, CT and Raman spectroscopy proved to be suitable techniques to characterize the structure and composition of hydrogels.
- Published
- 2021
13. Arthroscopic device with bendable tip for the controlled extrusion of hydrogels on cartilage defects.
- Author
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Guarnera D, Restaino F, Vannozzi L, Trucco D, Mazzocchi T, Worwąg M, Gapinski T, Lisignoli G, Zaffagnini S, Russo A, and Ricotti L
- Subjects
- Humans, Animals, Equipment Design, Hydrogels chemistry, Arthroscopy methods, Cartilage, Articular surgery, Chondrocytes
- Abstract
Advanced tools for the in situ treatment of articular cartilage lesions are attracting a growing interest in both surgery and bioengineering communities. The interest is particularly high concerning the delivery of cell-laden hydrogels. The tools currently available in the state-of-the-art hardly find an effective compromise between treatment accuracy and invasiveness. This paper presents a novel arthroscopic device provided with a bendable tip for the controlled extrusion of cell-laden hydrogels. The device consists of a handheld extruder and a supply unit that allows the extrusion of hydrogels. The extruder is equipped with a disposable, bendable nitinol tip (diameter: 4 mm, length: 92 mm, maximum bending angle: 90°) that guarantees access to hard-to-reach areas of the joint, which are difficult to get to, with conventional arthroscopic instruments. The tip accommodates a biocompatible polymer tube that is directly connected to the cartridge containing the hydrogel, whose plunger is actuated by a volumetric or pneumatic supply unit (both tested, in this study). Three different chondrocyte-laden hydrogels (RGD-modified Vitrogel®, methacrylated gellan gum, and an alginate-gelatine blend) were considered. First, the performance of the device in terms of resolution in hydrogel delivery was assessed, finding values in the range between 4 and 102 µL, with better performance found for the pneumatic supply unit and no significant differences between straight tip and bent tip conditions. Finite element simulations suggested that the shear stresses and pressure levels generated during the extrusion process were compatible with a safe deposition of the hydrogels. Biological analyses confirmed a high chondrocyte viability over a 7-day period after the extrusion of the three cell-laden hydrogel types, with no differences between the two supply units. The arthroscopic device was finally tested ex vivo by nine orthopedic surgeons on human cadaver knees. The device allowed surgeons to easily deliver hydrogels even in hard-to-reach cartilage areas. The outcomes of a questionnaire completed by the surgeons demonstrated a high usability of the device, with an overall preference for the pneumatic supply unit. Our findings provide evidence supporting the future arthroscopic device translation in pre-clinical and clinical scenarios, dealing with osteoarticular treatments., (© 2024. The Author(s).)
- Published
- 2024
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14. Ultrasound Stimulation of Piezoelectric Nanocomposite Hydrogels Boosts Chondrogenic Differentiation in Vitro , in Both a Normal and Inflammatory Milieu.
- Author
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Ricotti L, Cafarelli A, Manferdini C, Trucco D, Vannozzi L, Gabusi E, Fontana F, Dolzani P, Saleh Y, Lenzi E, Columbaro M, Piazzi M, Bertacchini J, Aliperta A, Cain M, Gemmi M, Parlanti P, Jost C, Fedutik Y, Nessim GD, Telkhozhayeva M, Teblum E, Dumont E, Delbaldo C, Codispoti G, Martini L, Tschon M, Fini M, and Lisignoli G
- Subjects
- Nanogels, Hydrogels pharmacology, Cell Differentiation, Tissue Engineering, Chondrogenesis, Proteomics
- Abstract
The use of piezoelectric nanomaterials combined with ultrasound stimulation is emerging as a promising approach for wirelessly triggering the regeneration of different tissue types. However, it has never been explored for boosting chondrogenesis. Furthermore, the ultrasound stimulation parameters used are often not adequately controlled. In this study, we show that adipose-tissue-derived mesenchymal stromal cells embedded in a nanocomposite hydrogel containing piezoelectric barium titanate nanoparticles and graphene oxide nanoflakes and stimulated with ultrasound waves with precisely controlled parameters (1 MHz and 250 mW/cm
2 , for 5 min once every 2 days for 10 days) dramatically boost chondrogenic cell commitment in vitro . Moreover, fibrotic and catabolic factors are strongly down-modulated: proteomic analyses reveal that such stimulation influences biological processes involved in cytoskeleton and extracellular matrix organization, collagen fibril organization, and metabolic processes. The optimal stimulation regimen also has a considerable anti-inflammatory effect and keeps its ability to boost chondrogenesis in vitro , even in an inflammatory milieu. An analytical model to predict the voltage generated by piezoelectric nanoparticles invested by ultrasound waves is proposed, together with a computational tool that takes into consideration nanoparticle clustering within the cell vacuoles and predicts the electric field streamline distribution in the cell cytoplasm. The proposed nanocomposite hydrogel shows good injectability and adhesion to the cartilage tissue ex vivo , as well as excellent biocompatibility in vivo, according to ISO 10993. Future perspectives will involve preclinical testing of this paradigm for cartilage regeneration.- Published
- 2024
- Full Text
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15. A Novel Approach for Multiple Material Extrusion in Arthroscopic Knee Surgery.
- Author
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Mazzocchi T, Guarnera D, Trucco D, Restaino FR, Vannozzi L, Siliberto A, Lisignoli G, Zaffagnini S, Russo A, and Ricotti L
- Subjects
- Humans, Knee Joint surgery, Arthroscopy, Cell Survival, Biocompatible Materials, Cartilage, Articular surgery, Cartilage, Articular pathology, Osteoarthritis, Knee pathology
- Abstract
Articular cartilage defects and degenerative diseases are pathological conditions that cause pain and the progressive loss of joint functionalities. The most severe cases are treated through partial or complete joint replacement with prostheses, even if the interest in cartilage regeneration and re-growth methods is steadily increasing. These methods consist of the targeted deposition of biomaterials. Only a few tools have been developed so far for performing these procedures in a minimally invasive way. This work presents an innovative device for the direct deposition of multiple biomaterials in an arthroscopic scenario. The tool is easily handleable and allows the extrusion of three different materials simultaneously. It is also equipped with a flexible tip to reach remote areas of the damaged cartilage. Three channels are arranged coaxially and a spring-based dip-coating approach allows the fabrication and assembly of a bendable polymeric tip. Experimental tests were performed to characterize the tip, showing the ability to bend it up to 90° (using a force of ~ 1.5 N) and to extrude three coaxial biomaterials at the same time with both tip straight and tip fully bent. Rheometric analysis and fluid-dynamic computational simulations were performed to analyze the fluids' behavior; the maximum shear stresses were observed in correspondence to the distal tip and the channel convergence chamber, but with values up to ~ 1.2 kPa, compatible with a safe extrusion of biomaterials, even laden with cells. The cells viability was assessed after the extrusion with Live/Dead assay, confirming the safety of the extrusion procedures. Finally, the tool was tested arthroscopically in a cadaveric knee, demonstrating its ability to deliver the biomaterial in different areas, even ones that are typically hard-to-reach with traditional tools., (© 2022. The Author(s).)
- Published
- 2023
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16. Primers for the Adhesion of Gellan Gum-Based Hydrogels to the Cartilage: A Comparative Study.
- Author
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Trucco D, Riacci L, Vannozzi L, Manferdini C, Arrico L, Gabusi E, Lisignoli G, and Ricotti L
- Subjects
- Cartilage, Catecholamines, Cellulose, Polysaccharides, Bacterial, Tissue Engineering methods, Fibrin Tissue Adhesive, Hydrogels pharmacology
- Abstract
A stable adhesion to the cartilage is a crucial requisite for hydrogels used for cartilage regeneration. Indeed, a weak interface between the tissue and the implanted material may produce a premature detachment and thus the failure of the regeneration processes. Fibrin glue, cellulose nanofibers and catecholamines have been proposed in the state-of-the-art as primers to improve the adhesion. However, no studies focused on a systematic comparison of their performance. This work aims to evaluate the adhesion strength between ex vivo cartilage specimens and polysaccharide hydrogels (gellan gum and methacrylated gellan gum), by applying the mentioned primers as intermediate layer. Results show that the fibrin glue and the cellulose nanofibers improve the adhesion strength, while catecholamines do not guarantee reaching a clinically acceptable value. Stem cells embedded in gellan gum hydrogels reduce the adhesion strength when fibrin glue is used as a primer, being anyhow still sufficient for in vivo applications., (© 2022 The Authors. Macromolecular Bioscience published by Wiley-VCH GmbH.)
- Published
- 2022
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17. RGD-Functionalized Hydrogel Supports the Chondrogenic Commitment of Adipose Mesenchymal Stromal Cells.
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Manferdini C, Trucco D, Saleh Y, Gabusi E, Dolzani P, Lenzi E, Vannozzi L, Ricotti L, and Lisignoli G
- Abstract
Articular cartilage is known to have limited intrinsic self-healing capacity when a defect or a degeneration process occurs. Hydrogels represent promising biomaterials for cell encapsulation and injection in cartilage defects by creating an environment that mimics the cartilage extracellular matrix. The aim of this study is the analysis of two different concentrations (1:1 and 1:2) of VitroGel
® (VG) hydrogels without (VG-3D) and with arginine-glycine-aspartic acid (RGD) motifs, (VG-RGD), verifying their ability to support chondrogenic differentiation of encapsulated human adipose mesenchymal stromal cells (hASCs). We analyzed the hydrogel properties in terms of rheometric measurements, cell viability, cytotoxicity, and the expression of chondrogenic markers using gene expression, histology, and immunohistochemical tests. We highlighted a shear-thinning behavior of both hydrogels, which showed good injectability. We demonstrated a good morphology and high viability of hASCs in both hydrogels. VG-RGD 1:2 hydrogels were the most effective, both at the gene and protein levels, to support the expression of the typical chondrogenic markers, including collagen type 2, SOX9, aggrecan, glycosaminoglycan, and cartilage oligomeric matrix protein and to decrease the proliferation marker MKI67 and the fibrotic marker collagen type 1. This study demonstrated that both hydrogels, at different concentrations, and the presence of RGD motifs, significantly contributed to the chondrogenic commitment of the laden hASCs.- Published
- 2022
- Full Text
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18. Modeling and Fabrication of Silk Fibroin-Gelatin-Based Constructs Using Extrusion-Based Three-Dimensional Bioprinting.
- Author
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Trucco D, Sharma A, Manferdini C, Gabusi E, Petretta M, Desando G, Ricotti L, Chakraborty J, Ghosh S, and Lisignoli G
- Subjects
- Cells, Cultured, Chondrogenesis, Gelatin, Humans, Hydrogels, Mesenchymal Stem Cells, Tissue Engineering, Bioprinting, Fibroins
- Abstract
Robotic dispensing-based 3D bioprinting represents one of the most powerful technologies to develop hydrogel-based 3D constructs with enormous potential in the field of regenerative medicine. The optimization of hydrogel printing parameters, proper geometry and internal architecture of the constructs, and good cell viability during the bioprinting process are the essential requirements. In this paper, an analytical model based on the hydrogel rheological properties was developed to predict the extruded filament width in order to maximize the printed structure's fidelity to the design. Viscosity data of two natural hydrogels were imputed to a power-law model to extrapolate the filament width. Further, the model data were validated by monitoring the obtained filament width as the output. Shear stress values occurring during the bioprinting process were also estimated. Human mesenchymal stromal cells (hMSCs) were encapsulated in the silk fibroin-gelatin (G)-based hydrogel, and a 3D bioprinting process was performed to produce cell-laden constructs. Live and dead assay allowed estimating the impact of needle shear stress on cell viability after the bioprinting process. Finally, we tested the potential of hMSCs to undergo chondrogenic differentiation by evaluating the cartilaginous extracellular matrix production through immunohistochemical analyses. Overall, the use of the proposed analytical model enables defining the optimal printing parameters to maximize the fabricated constructs' fidelity to design parameters before the process execution, enabling to achieve more controlled and standardized products than classical trial-and-error approaches in the biofabrication of engineered constructs. Employing modeling systems exploiting the rheological properties of the hydrogels might be a valid tool in the future for guaranteeing high cell viability and for optimizing tissue engineering approaches in regenerative medicine applications.
- Published
- 2021
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19. Graphene Oxide-Doped Gellan Gum-PEGDA Bilayered Hydrogel Mimicking the Mechanical and Lubrication Properties of Articular Cartilage.
- Author
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Trucco D, Vannozzi L, Teblum E, Telkhozhayeva M, Nessim GD, Affatato S, Al-Haddad H, Lisignoli G, and Ricotti L
- Published
- 2021
- Full Text
- View/download PDF
20. Wear Behavior Characterization of Hydrogels Constructs for Cartilage Tissue Replacement.
- Author
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Affatato S, Trucco D, Taddei P, Vannozzi L, Ricotti L, Nessim GD, and Lisignoli G
- Abstract
This paper aims to characterize the wear behavior of hydrogel constructs designed for human articular cartilage replacement. To this purpose, poly (ethylene glycol) diacrylate (PEGDA) 10% w / v and gellan gum (GG) 1.5% w / v were used to reproduce the superior (SUP) cartilage layer and PEGDA 15% w / v and GG 1.5% w / v were used to reproduce the deep (DEEP) cartilage layer, with or without graphene oxide (GO). These materials (SUP and DEEP) were analyzed alone and in combination to mimic the zonal architecture of human articular cartilage. The developed constructs were tested using a four-station displacement control knee joint simulator under bovine calf serum. Roughness and micro-computer tomography (µ-CT) measurements evidenced that the hydrogels with 10% w / v of PEGDA showed a worse behavior both in terms of roughness increase and loss of uniformly distributed density than 15% w / v of PEGDA. The simultaneous presence of GO and 15% w / v PEGDA contributed to keeping the hydrogel construct's characteristics. The Raman spectra of the control samples showed the presence of unreacted C=C bonds in all the hydrogels. The degree of crosslinking increased along the series SUP < DEEP + SUP < DEEP without GO. The Raman spectra of the tested hydrogels showed the loss of diacrylate groups in all the samples, due to the washout of unreacted PEGDA in bovine calf serum aqueous environment. The loss decreased along the series SUP > DEEP + SUP > DEEP, further confirming that the degree of photo-crosslinking of the starting materials plays a key role in determining their wear behavior. μ-CT and Raman spectroscopy proved to be suitable techniques to characterize the structure and composition of hydrogels.
- Published
- 2021
- Full Text
- View/download PDF
21. Impact of Isolation Procedures on the Development of a Preclinical Synovial Fibroblasts/Macrophages in an In Vitro Model of Osteoarthritis.
- Author
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Manferdini C, Saleh Y, Dolzani P, Gabusi E, Trucco D, Filardo G, and Lisignoli G
- Abstract
There is a lack ofin vitromodels able to properly represent osteoarthritis (OA) synovial tissue (ST). We aimed to characterize OA ST and to investigate whether a mechanical or enzymatic digestion procedures influence synovial cell functional heterogeneity in vitro. Procedures using mechanical nondigested fragments (NDF), synovial digested fragments (SDF), and filtrated synovial digested cells (SDC) were compared. An immunophenotypic profile was performed to distinguish synovial fibroblasts (CD55, CD73, CD90, CD106), macrophages (CD14, CD68), M1-like (CD80, CD86), and M2-like (CD163, CD206) synovial macrophages. Pro-inflammatory (interleukin 6 IL6), tumor necrosis factor alpha (TNFα), chemokine C-C motif ligand 3 (CCL3/MIP1α), C-X- motif chemokine ligand 10 (CXCL10/IP10) and anti-inflammatory (interleukin 10 (IL10)), transforming growth factor beta 1 (TGFβ1), C-C motif chemokine ligand 18 (CCL18) cytokines were evaluated. CD68 and CD163 markers were higher in NDF and SDF compared to the SDC procedure, while CD80, CD86, and CD206 were higher only in NDF compared to the SDC procedure. Synovial fibroblast markers showed similar percentages. TNFα, CCL3/MIP1α, CXCL10/IP10, and CCL18 were higher in NDF compared to SDC, but not compared to SDF. IL10 and TGFβ1 were higher in NDF than SDC at the molecular level, while IL6 did not show differences among procedures. We demonstrated that NDF isolation procedures better preserved the heterogeneity of specific OA synovial populations (fibroblasts, macrophages), fostering their use for testing new cell therapies or drugs for OA, reducing or avoiding the use of animal models.
- Published
- 2020
- Full Text
- View/download PDF
22. Investigating the Role of Sustained Calcium Release in Silk-Gelatin-Based Three-Dimensional Bioprinted Constructs for Enhancing the Osteogenic Differentiation of Human Bone Marrow Derived Mesenchymal Stromal Cells.
- Author
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Sharma A, Desando G, Petretta M, Chawla S, Bartolotti I, Manferdini C, Paolella F, Gabusi E, Trucco D, Ghosh S, and Lisignoli G
- Abstract
Scaffold-based bone tissue engineering strategies fail to meet the clinical need to fabricate patient-specific and defect shape-specific, anatomically relevant load-bearing bone constructs. 3D bioprinting strategies are gaining major interest as a potential alternative, but design of a specific bioink is still a major challenge that can modulate key signaling pathways to induce osteogenic differentiation of progenitor cells, as well as offer appropriate microenvironment to augment mineralization. In the present study, we developed silk fibroin protein and gelatin-based conjugated bioink, which showed localized presence and sustained release of calcium. Presence of 2.6 mM Ca
2+ ions within the bioink could further induce enhanced osteogenesis of Bone marrow derived progenitor cells (hMSCs) compared to the bioink without calcium, or same concentration of calcium added to the media, as evidenced by upregulated gene expression of osteogenic markers. This study generated unprecedented mechanistic insights on the role of fibroin-gelatin-CaCl2 bioink in modulating expression of several proteins which are known to play crucial role in bone regeneration as well as key signaling pathways such as β-catenin, BMP signaling pathway, Parathyroid hormone-dependent signaling pathway, Forkhead box O (FOXO) pathway, and Hippo pathways in hMSC-laden bioprinted constructs.- Published
- 2019
- Full Text
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23. Feasibility of a home care program in a pediatric hematology and oncology department. Results of the first year of activity at a single Institution.
- Author
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Miano M, Manfredini L, Garaventa A, Fieramosca S, Tanasini R, Leimer M, Trucco D, Rossi R, Haupt R, and Dini G
- Subjects
- Adolescent, Adult, Child, Child, Preschool, Feasibility Studies, Female, Home Care Services, Hospital-Based economics, Home Care Services, Hospital-Based standards, Hospitals, Pediatric organization & administration, Humans, Infant, Infant, Newborn, Male, Oncology Service, Hospital economics, Oncology Service, Hospital standards, Surveys and Questionnaires, Home Care Services, Hospital-Based organization & administration, Neoplasms therapy, Oncology Service, Hospital organization & administration
- Abstract
Background and Objectives: Giannina Gaslini Children's Hospital (GGCH) is a tertiary care hospital with an average of 100 new cancer diagnoses made each year. In April 2000, following preliminary analysis of the potential benefits, and the results of a questionnaire filled in by the parents attending the out-patient clinic, a Home Care (HC) program was started., Design and Methods: Children in stable, non-critical, clinical conditions requiring i.v. therapy, parenteral nutrition, transfusional support, blood examinations, and central venous catheter use training management, as well as terminally ill children needing palliative and support therapy were considered eligible for the program., Results: After one year of activity, 45 children, aged 1 month-19 years (median 3 years), requiring i.v. therapy and blood tests in 32 cases, central venous catheter use training in 5, and palliative care in 8, were treated at home. The median duration of assistance for each child was 19 days (range 1-172). An average of 4 patients per week were assisted for a total of 1,364 days. A total of 881 accesses at home replaced 551 and 330 out-patient and in-patient days of hospitalization, respectively. The average cost per patient given home care (2,936 E, range 150-20,700) resulted to be significantly lower than the average cost per patient hospitalized to undergo the same procedures (9,785E, range 350-96,750)., Interpretation and Conclusions: The opportunity to reduce the frequency and duration of hospitalization represents an incalculable advantage for these children and their families. This report shows that home care is a feasible kind of assistance for children suffering from cancer, and reduces costs as well.
- Published
- 2002
24. [Occupational stress in health care personnel].
- Author
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Trucco M, Valenzuela P, and Trucco D
- Subjects
- Adult, Burnout, Professional diagnosis, Chile, Female, Humans, Job Satisfaction, Male, Occupational Diseases diagnosis, Risk Factors, Sex Factors, Surveys and Questionnaires, Burnout, Professional psychology, Health Personnel psychology, Occupational Diseases psychology
- Abstract
Background: Occupational stress results from the interaction of multiple risk factors, such as the physical environment, biological function disturbances, work content and organization and diverse psychosocial components., Aim: To study the frequency of symptoms and the main sources of job stress, perceived by professional and non professional health care workers and to compare gender differences., Subjects and Methods: A group of specially designed, self-administered, questionnaires adapted by the main author and independently validated, were applied. The results of two symptoms, one work satisfaction, and one job stress scales are reported., Results: One hundred sixteen women and 89 men were studied, 143 are professionals and 62 non professionals (clerical and nurse auxiliaries). Forty percent had symptoms of job stress and 82 subjects were defined as "probable cases", according to the ratings on the symptom scales. There were significant differences in the frequency of symptoms between professionals and non professionals (34.3 and 69.2% respectively). The main differences in symptoms, sources of job stress and dissatisfaction were more closely associated to the occupational level than to gender., Conclusions: A high frequency of job stress symptoms was observed in this sample of health care workers and the risk of occupational stress varies more with the occupational level than with gender.
- Published
- 1999
25. [Sepsis in children with malignant neoplasia, equipped with a Broviac-type venous catheter].
- Author
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Garaventa A, Castagnola E, Dallorso S, Dini G, Trucco D, Vianello O, Carrega G, Cuneo P, Buffa P, and Magillo P
- Subjects
- Antineoplastic Agents administration & dosage, Catheterization, Central Venous instrumentation, Child, Female, Gram-Negative Bacteria isolation & purification, Gram-Positive Bacteria isolation & purification, Humans, Infusions, Intravenous, Male, Neutropenia etiology, Parenteral Nutrition instrumentation, Sepsis microbiology, Catheterization, Central Venous adverse effects, Catheters, Indwelling adverse effects, Neoplasms therapy, Sepsis etiology
- Abstract
Indwelling central venous catheters obviate many problems in the care of children with malignancies, but they also are a well-known source of infection. We are reviewed the history of 584 Broviac catheters inserted from January 1984 to December 1991, in 475 children with cancer in order to assess the etiology of bacteremias, their association with neutropenia and their relationship with the presence of the catheters. The overall duration-time of the catheters, employed for blood tests, drug and blood infusions and parenteral nutrition, was 1-835 days (median 263, mean 186). Total catheter courses was 108.678 days. In this period 226 episodes of sepsis were observed in 180 patients: 157 in neutropenic patients and 69 in non neutropenic. Catheter related bacteremias were diagnosed in 65/226 episodes (29%): 23 (35%) were observed in neutropenic patients and 42 (65%) in non neutropenic (P < 0.005). Gram-positive pathogens were isolated in 28/65 (43%) episodes, Gram-negatives in 15/65 (23%), fungi in 9/65 (14%), and the remaining 13 (20%) were polymicrobial. In the last years we observed an increase of catheter related bacteremias due to Gram-negative rods no change was observed in pathogens causing catheters unrelated bacteremias. The high incidence of catheters related bacteremias in non neutropenic, non hospitalized patients, stress on the home-care of the catheters; a high level of suspicion of Gram-negative infections should be maintained in cancer patients with an indwelling central venous catheters.
- Published
- 1995
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