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We previously studied ATPasic activity in the lymphocyte according to sex, age and in pregnant women. Now we considered it is interesting to go on with out study with the dosage of ATPasic lymphocytic activity during menstrual cycle, monitored with basal temperature method. The methodology we followed is Ellegaard and Dimitrov one of 1972, with modifications and simplifications while the proteins dosage in the supernatant has been effected with biuret method. Our data showed a decrease of the ATPasic lymphocytic activity at the middle of cycle for ovulatory cycles, while there is no difference between data at the middle of the cycle and in menstruation for cycle without ovulation. We suppose a connection between ATPasic lymphocytic activity and the hormones which stimulate ovulation.

Through the use of the Limulus test research has been carried out on gram-negative endotoxin in patients with hepatic cirrhosis, chronic hepatitis, acute hepatitis, and in a control group. The positivity of this test in patients with cirrhosis and chronic hepatitis was 93.3% and in cases of acute hepatitis it was 90.9%. The effect of the combined administration of lactulose and paromomycin on endotoxin blood levels has been evaluated in a group of 9 patients with acute hepatitis, 8 with cirrhosis, 1 in hepatic coma, and 1 patient with chronic persistent hepatitis: in 18 of the 19 patients the Limulus test became negative. The results have been discussed in relation to clinical and laboratory data, and to recent data concerning the interaction between intestinal bacterial flora, endotoxin, and liver. Hypotheses have been proposed regarding the hepatocellular c-AMP mediated mechanism of endotoxin action.

Gastric juice proteins were studied in normal subjects and persons with gastro-intestinal, lung and pancreatic neoplasia and chronic systemic lymphopathy and haemoblastosis, using cellulose acetate and acrylamide gel electrophoresis of the freeze-dried dialyaste and chromatography of the amino acids obtained from the hydrolysate. Definite evidence was gathered of both qualitative and quantitative changes in protein fractions. Patterns varied from one group of subjects to another, though the basic picture was the same in both intestinal and extra-intestinal conditions. An effective relationship between the diseases considered is postulated and the data are interpreted with a view to evolving other points for further investigation.

Membrane proteins in circulating blood cells were examined in chick embryos on the 4th, 5th, 6th, 7th and 10th incubation day, in chicks immediately after hatching, and in adult birds. Haemoglobin electrophoresis patterns were determined on cellulose acetate after solubilisation with urea, and in gradient of pH and concentration on acrylamide gels after solubilisation in SDS. Amino acids and other amino compounds were also determined after acid hydrolysis and resin chromatography. Haemoglobin patterns were similar to those displayed by the membrane proteins. The latter, however, changed more slowly.

The object we propose to ourselves is to define mean values of ATPasic lymphocytic activity in different physiological conditions. This study considers the ATPasic lymphocytic activity in the woman at the end of prolific age. The methodology we followed is Ellegaard and Dimitrov one of 1972 with some modifications and simplifications; the proteins dosage in the overfloating has been effected with biuret method. No correlation between the number of months from last reported menstruation and the ATPasic lymphocytic activity has come out. Furthermore, the data woman at the end od fertile age similar to the ones of the young woman in menstrual cycle or at the middle of a cycle without ovulation. Data of aged woman, on the contrary, draw away from the data of young woman at the middle of an ovulatory cycle. Our present intention is, at this point, to turn attention to peculiar hormonal situations, for instance in presence of an estroprogestinic therapy.