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Additional file 3: Supplementary Figure S3. ErbB3 localizes in the nucleolus of U-87MG probed with an anti ErbB3 RTJ2 monoclonal antibody after 24h of serum starvation (0.2% FBS). ErbB3 is shown in green and Fibrillarin in red. Scalebar represent 5 ��m.

Additional file 4: Supplementary Figure 4. The 50 kDa variant of ErbB3 is detected in MCF-7 cells. (A) SDS-page of whole cells lysate from U-87MG and MCF-7. Approximatively 40 ��g were loaded on each lane. (B) Quantification of ErbB3 expression. Data is the mean of three independent experiments. (** p

Additional file 1: Supplementary Figure 1. (A) ErbB3 localizes in the nucleolus of HeLa and MCF-7 cells. Immunofluorescence analysis for ErbB3 (green) and Fibrillarin (red) after 24h of serum starvation (0.2% FBS). (B). Confocal magnifications and relative lateral sections are shown HeLa cells. (C) ErbB3 localization in MCF-7. The staining was performed using rabbit polyclonal anti-ErbB3 C17 (green), anti Pol I subunit RPA40 (red). The colocalization is shown in yellow. Scalebar represent 10 ��m in A and 25 ��m in C.

Additional file 7: Supplementary Figure 7. Immunohistochemistry of glioblastoma patient biopsies. ErbB3 is stained in brown and the nuclei are in blue.

Additional file 6: Supplementary Figure 6. Difference in medium pH of U-87MG after 72h exposure to actinomycin D.

Additional file 8:. Supplementary information.

La temperatura corporea costituisce un importante meccanismo di difesa ed � il risultato di una complessa interazione che coinvolge numerosi fattori. Nell'uomo sano, la temperatura corporea � finemente regolata; deviazioni di 0.5�C oltre il limite superiore possono indicare una condizione patologica. Numerosi agenti possono indurre ipertermia, tra cui, insufficienza cardiaca acuta/cardiomiopatia acuta da stress [1] e infarto miocardico acuto [2] sindrome neurolettica maligna [3], endocrinopatie [4, 5], disturbi del sistema nervoso centrale (SNC) [6] e patologie oncologiche [7]. Le temperature febbrili aumentano l'efficacia della risposta immunitaria durante le infezioni stimolando il sistema immunitario innato e adattativo. Questo studio ha come obiettivo quello di dimostrare come l'ipertermia possa indurre modifiche del profilo di espressione genica e di evidenziare nuovi marker precoci di prognosi/diagnosi in patologie autoimmuni e/o tumorali. Nelle cellule dendritiche, alcuni tra i geni up-regolati codificano per proteine secrete, come IGFBP6 [8]. In condizioni ipertermiche, questa proteina induce chemiotassi dei monociti, dei linfociti T, ma non dei linfociti B. Inoltre, IGFBP6 � un agonista selettivo nei neutrofili poich� aumenta sia il burst ossidativo che la degranulazione e agisce come fattore chemotattico.

Polymeric nanostructured biomaterials can be used as synthetic cell interfaces with important applications in the study and control of cellular processes. Herein, we developed multifunctional nanocomposites based on synthesized biodegradable and biocompatible copolyesters of poly(butylene 1,4-trans-cyclohexanedicarboxylate) (PBCE) containing ether–linkages, and single walled carbon nanotubes (SWCNTs), employed as functional phase. Surface, thermal and mechanical characterization of the polymer and nanocomposite films were performed. The influences of AC conductivity and interfacial polarization on dielectric relaxation process, as well as the correlation between the dielectric behaviors and SWCNT content were investigated by measuring the dielectric properties. The effect of SWCNT incorporation, and amount of ether-oxygen atoms was also investigated in terms of fibroblast long-term culture stability, by performing adhesion and proliferation studies of cells seeded on the biomaterial surface, at different time points. Results showed that polymeric conductive nanocomposites were successfully developed with a low percolation threshold, and SWCNT presence maintained the polymer thermal degradation behavior. Moreover, the culture of primary fibroblasts indicated that these advanced functional materials are biocompatible and guarantee the cell adhesion and growth, being suitable substrates for regenerative medicine applications. Finally, their versatile structure and chemical properties may provide a robust platform to gain insight into cell–biomaterial interactions, being an important step towards the better understanding and control of cell interactions with nanomaterials.